Standard name
Human Ortholog
Description Protein involved in G2/M phase progression and response to DNA damage; interacts with Rad53p; contains an RNA recognition motif, a nuclear localization signal, and several SQ/TQ cluster domains; hyperphosphorylated in response to DNA damage
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0.05 0 0.07 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0.05 0 0 0.07 0 0.09 0 0 0 0.09 0 0.1 0 0 0 0 0 0
Cytoplasm 0.97 0.98 0.98 0.91 0.94 0.93 0.82 0.81 0.76 0.73 0.64 0.65 0.98 1.0 0.99 0.87 0.88 0.92 0.94 0.9 0.77 0.95 0.96 0.92
Endoplasmic Reticulum 0 0 0 0 0.06 0.05 0.1 0.11 0.12 0.08 0.09 0.11 0 0 0 0 0 0.05 0 0 0.06 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0.05 0.06 0.09 0.09 0.31 0.19 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0.05 0 0 0 0 0 0.08 0 0.05 0 0 0 0 0.08 0.06 0 0 0.08 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 1 0 0 5 3 5 12 3 15 0 0 2 0 0 0 0 1 0 0 0 0
Bud 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 2 3 2 4 3 9 14 8 8 17 6 21 4 1 7 4 2 6 0 0 0 0 0 0
Cytoplasm 157 185 47 101 159 210 223 237 147 181 122 148 131 162 222 41 97 58 147 176 59 87 142 193
Endoplasmic Reticulum 2 3 1 2 10 12 27 33 23 19 18 25 4 0 1 1 3 3 6 2 4 0 2 4
Endosome 0 0 0 0 1 0 0 0 0 1 0 1 0 0 0 0 0 0 0 0 1 0 0 1
Golgi 1 0 0 0 1 0 4 3 0 2 0 1 0 0 1 1 1 0 0 1 0 0 0 1
Mitochondria 2 2 0 2 0 2 15 18 17 23 60 43 0 0 1 2 2 0 1 1 2 0 1 1
Nucleus 0 1 1 2 2 1 1 0 0 1 0 3 0 0 1 0 2 0 0 1 0 1 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 1 0 6 2 2 6 11 4 21 7 12 0 1 0 1 9 4 1 6 6 1 1 3
Unique Cell Count 162 188 48 111 170 225 273 293 193 248 192 228 133 162 225 47 110 63 158 195 77 92 149 211
Labelled Cell Count 164 195 51 119 178 236 295 313 206 277 216 269 139 164 235 51 116 71 158 195 77 92 149 211


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 8.7 10.6 10.3 10.7 12.3 10.7 10.3 10.6 9.4 10.1 8.7 9.4 11.9 10.8 11.3 12.0 12.7 13.2 10.6 11.0 11.3
Std Deviation (1e-4) 1.1 2.1 1.6 1.8 2.1 1.7 1.9 1.8 1.6 2.0 1.7 1.8 1.7 1.6 1.5 2.3 2.8 2.6 1.5 2.0 1.8
Intensity Change (Log2) – – – 0.05 0.25 0.05 0.0 0.04 -0.13 -0.03 -0.25 -0.14 0.2 0.07 0.13 0.22 0.3 0.35 0.03 0.1 0.13


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm -1.6 -1.2 -1.2 -2.8 -2.9 -3.4 -3.8 -4.7 -4.6 0.3 1.8 0.4 -2.0 -2.0 -1.4
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 4.5 3.3 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 5.7599 10.2741 8.861 8.0226 7.1192 8.2924 7.7525 11.3971 9.6104 9.1284 8.6573 10.2173 9.969 12.7128 11.0271 11.0439 11.4478 10.9578
Actin 0.0294 0.0147 0.0084 0.0119 0.0331 0.0029 0.0036 0.001 0.0232 0.007 0.0031 0.0011 0.0024 0.0005 0.0013 0.0003 0.0001 0.0022
Bud 0.0023 0.0004 0.0009 0.0008 0.0007 0.0006 0.0006 0.0003 0.0011 0.0011 0.0009 0.0004 0.0003 0.0003 0.0003 0.0007 0.0001 0.0003
Bud Neck 0.0028 0.0005 0.001 0.0007 0.001 0.0019 0.0005 0.0002 0.0007 0.0006 0.0012 0.0011 0.0003 0.0003 0.0004 0.0007 0.0003 0.0011
Bud Periphery 0.0032 0.0002 0.0007 0.0006 0.0005 0.0005 0.0005 0.0001 0.0006 0.0013 0.0015 0.0003 0.0002 0.0001 0.0001 0.0003 0 0.0002
Bud Site 0.0146 0.0025 0.0033 0.0012 0.0009 0.0007 0.0012 0.0009 0.0103 0.0007 0.0015 0.0001 0.0012 0.0007 0.0005 0.0002 0.0001 0.0001
Cell Periphery 0.0012 0.0003 0.0008 0.0002 0.0001 0.0014 0.0009 0.0002 0.0004 0.0002 0.0002 0.0002 0.0003 0.0003 0.0002 0.0001 0 0.0001
Cytoplasm 0.5562 0.9494 0.8333 0.6064 0.772 0.7941 0.7562 0.9405 0.7997 0.6725 0.7347 0.8835 0.8621 0.9347 0.879 0.8221 0.9152 0.8943
Cytoplasmic Foci 0.0847 0.0058 0.0164 0.019 0.0279 0.0219 0.0427 0.0091 0.026 0.0549 0.029 0.0161 0.0133 0.0083 0.0133 0.0141 0.0117 0.0114
Eisosomes 0.0005 0 0.0005 0.0002 0.0001 0 0 0 0.0001 0.0001 0 0 0 0 0 0 0 0
Endoplasmic Reticulum 0.0282 0.0066 0.0373 0.0236 0.0101 0.0315 0.0287 0.0131 0.0204 0.0153 0.0171 0.0319 0.0512 0.0187 0.0318 0.0112 0.007 0.0383
Endosome 0.0989 0.0102 0.0471 0.1492 0.0637 0.0815 0.09 0.0142 0.054 0.1075 0.1172 0.039 0.0395 0.0191 0.0461 0.0824 0.0516 0.0359
Golgi 0.0228 0.0025 0.0114 0.0157 0.0514 0.0249 0.0156 0.0036 0.014 0.0151 0.0455 0.0088 0.0061 0.0049 0.0039 0.0072 0.0011 0.0051
Lipid Particles 0.021 0.0002 0.0012 0.0029 0.0021 0.0056 0.0127 0.0002 0.0068 0.012 0.002 0.0002 0.0009 0.0002 0.0014 0.0001 0 0.0001
Mitochondria 0.006 0.0009 0.0169 0.0615 0.0226 0.0188 0.0058 0.0009 0.0131 0.0299 0.0208 0.0083 0.0019 0.0016 0.0011 0.006 0.0004 0.0019
None 0.0271 0.0004 0.0007 0.0005 0.0006 0.0005 0.0024 0.0007 0.0014 0.0044 0.0021 0.0008 0.0013 0.0005 0.0007 0.0007 0.0005 0.0006
Nuclear Periphery 0.0193 0.0008 0.0035 0.0093 0.0012 0.0018 0.0024 0.0102 0.0016 0.0032 0.0015 0.001 0.0031 0.0008 0.0023 0.0049 0.002 0.0018
Nucleolus 0.0028 0 0.0001 0.0001 0 0.0001 0.0001 0 0.0001 0.0001 0.0005 0 0 0 0 0 0 0
Nucleus 0.0276 0.0007 0.0012 0.0024 0.001 0.0013 0.0011 0.0017 0.0012 0.0015 0.0027 0.0009 0.0014 0.0007 0.0011 0.002 0.0019 0.0013
Peroxisomes 0.0107 0.0005 0.0009 0.0016 0.004 0.0019 0.0145 0 0.0091 0.0133 0.001 0.0003 0.0015 0.0001 0.0025 0.0001 0 0.0001
Punctate Nuclear 0.0172 0.0001 0.0002 0.0002 0.0002 0.0005 0.0017 0.0001 0.0006 0.001 0.0016 0.0001 0.0005 0.0001 0.0004 0.0001 0.0001 0.0001
Vacuole 0.019 0.0025 0.0117 0.0638 0.0048 0.0055 0.0158 0.0024 0.0132 0.046 0.0123 0.0043 0.0105 0.0074 0.0108 0.037 0.0055 0.0038
Vacuole Periphery 0.0045 0.0005 0.0026 0.0281 0.0021 0.0021 0.003 0.0005 0.0023 0.0124 0.0036 0.0016 0.0019 0.0008 0.0027 0.01 0.0022 0.0013

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 49.7549 44.2321 48.5038 66.6554 54.8865 56.0463 74.0415 67.9163 59.6115 81.1395
Translational Efficiency 1.7303 1.9927 1.5043 0.9984 1.4187 1.8242 1.2946 1.4225 1.5938 1.408

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1757 1555 1971 1108 816 1775 154 1310 2573 3330 2125 2418

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 925.58 1100.09 1360.09 1368.73 1068.74 1083.56 1424.34 1344.73 970.98 1091.28 1364.75 1355.73
Standard Deviation 115.66 169.93 217.36 203.66 144.14 156.74 149.71 216.06 141.99 163.24 213.83 210.81
Intensity Change Log 2 — 0.249192 0.555273 0.564408 — 0.019868 0.414383 0.331406 — 0.130845 0.481485 0.444237

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000185 0.000326 0.000340 0.000932 0.000383 0.000522 0.000823 0.000673 0.000248 0.000430 0.000375 0.000792
Bud Neck 0.002496 0.000514 0.000844 0.001773 0.000854 0.000944 0.002741 0.001296 0.001975 0.000743 0.000982 0.001515
Bud Site 0.020100 0.024778 0.011099 0.034078 0.025828 0.026156 0.027359 0.025571 0.021917 0.025513 0.012277 0.029469
Cell Periphery 0.000206 0.000220 0.000179 0.000473 0.000261 0.000143 0.000266 0.000355 0.000223 0.000179 0.000185 0.000409
Cytoplasm 0.597514* 0.718967* 0.867372* 0.800406* 0.597084* 0.618310* 0.521840* 0.821056* 0.597377* 0.665314* 0.842331* 0.811593*
Cytoplasmic Foci 0.263883* 0.164598 0.039179 0.058451 0.276755* 0.231747* 0.167257 0.061722 0.267965* 0.200391* 0.048461 0.060223
Eisosomes 0.000044 0.000008 0.000003 0.000008 0.000022 0.000014 0.000019 0.000006 0.000037 0.000011 0.000004 0.000007
Endoplasmic Reticulum 0.009093 0.007107 0.002424 0.000410 0.014537 0.005195 0.003252 0.000734 0.010819 0.006088 0.002484 0.000585
Endosome 0.017287 0.009862 0.004380 0.005560 0.015660 0.017107 0.028205 0.005633 0.016771 0.013724 0.006106 0.005600
Golgi 0.021527 0.020098 0.003657 0.009665 0.026459 0.025218 0.003419 0.006298 0.023091 0.022827 0.003639 0.007841
Lipid Particles 0.003676 0.001065 0.002980 0.004295 0.002145 0.001173 0.006954 0.004116 0.003191 0.001123 0.003268 0.004198
Mitochondria 0.004804 0.002424 0.000083 0.000185 0.000869 0.002090 0.000011 0.000127 0.003556 0.002246 0.000078 0.000154
Mitotic Spindle 0.000442 0.000900 0.003650 0.007396 0.001684 0.004194 0.003419 0.007003 0.000836 0.002656 0.003633 0.007183
None 0.001163 0.000526 0.000702 0.000193 0.000292 0.000679 0.000067 0.000343 0.000887 0.000608 0.000656 0.000274
Nuclear Periphery 0.000070 0.000058 0.000098 0.000126 0.000032 0.000048 0.000198 0.000030 0.000058 0.000053 0.000105 0.000074
Nuclear Periphery Foci 0.000321 0.000846 0.003530 0.001587 0.000644 0.000413 0.010260 0.001464 0.000423 0.000615 0.004018 0.001520
Nucleolus 0.000144 0.000061 0.000095 0.000113 0.000049 0.000064 0.000061 0.000107 0.000114 0.000062 0.000092 0.000110
Nucleus 0.004882 0.002623 0.010776 0.004049 0.001986 0.002164 0.005597 0.004241 0.003964 0.002378 0.010401 0.004153
Peroxisomes 0.001133 0.000368 0.000180 0.001292 0.000510 0.000801 0.000856 0.000866 0.000935 0.000598 0.000229 0.001061
Vacuole 0.035980 0.025899 0.045609 0.066116 0.022442 0.043979 0.215617* 0.055867 0.031687 0.035536 0.057930 0.060563
Vacuole Periphery 0.015052 0.018752 0.002820 0.002892 0.011506 0.019040 0.001780 0.002493 0.013927 0.018906 0.002744 0.002676

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -3.80 -4.59 -3.21 -2.54 -2.27 -0.64 -3.87 -1.18 -0.44 0.57 -1.58 -3.98 -3.21 -1.70 -2.13
Bud Neck 12.00 8.74 2.30 -10.88 -7.00 -1.22 -8.89 -5.26 -4.58 6.23 10.54 6.65 2.12 -11.24 -5.68
Bud Site -3.14 6.87 -5.77 -3.45 -9.37 -0.17 -0.44 0.47 0.71 0.80 -3.12 7.95 -4.55 -2.04 -10.32
Cell Periphery -0.87 1.33 -6.72 -6.16 -7.18 6.46 0.30 -1.94 -5.08 -1.97 3.96 2.39 -6.10 -7.80 -7.10
Cytoplasm -13.06 -33.97 -20.96 -7.37 8.77 -1.76 3.62 -18.95 -21.36 -13.30 -9.34 -33.09 -29.65 -20.59 4.85
Cytoplasmic Foci 15.08 43.98 37.80 20.70 -5.80 4.94 9.23 26.78 30.39 9.69 12.67 49.11 46.53 36.22 -4.07
Eisosomes 13.15 14.85 12.82 -0.43 -6.28 3.34 0.84 8.03 5.41 8.24 12.28 16.29 15.01 4.68 -4.36
Endoplasmic Reticulum 1.31 5.39 7.63 6.82 3.95 4.47 5.83 7.13 5.69 4.07 4.04 7.66 10.31 8.86 3.92
Endosome 7.31 15.26 13.04 5.67 -1.36 -1.11 -3.74 8.47 13.29 7.67 3.71 14.54 15.76 13.85 1.65
Golgi 1.14 23.03 7.20 6.03 -2.88 0.76 19.37 15.35 13.95 -3.26 0.26 29.95 15.08 13.60 -3.94
Lipid Particles 5.48 0.68 -1.34 -9.28 -2.70 5.46 -6.01 -3.27 -4.92 2.68 6.25 -0.99 -2.31 -8.57 -1.76
Mitochondria 2.12 5.82 5.70 2.88 -1.31 -3.60 9.73 7.83 5.97 -2.93 1.91 6.26 6.13 5.19 -1.80
Mitotic Spindle -3.91 -5.62 -3.95 -3.69 -1.89 -2.15 -1.76 -3.00 -1.36 -1.41 -3.18 -5.07 -5.22 -3.42 -2.39
None 3.47 2.60 5.48 4.90 6.57 -1.13 3.36 -0.44 1.02 -4.06 1.29 1.86 4.90 1.84 5.14
Nuclear Periphery 0.54 -2.04 -0.63 -0.79 -0.12 -1.09 -6.08 -0.28 1.01 6.15 0.35 -3.79 -0.42 -0.57 1.20
Nuclear Periphery Foci -5.12 -9.52 -7.08 -4.15 4.88 1.22 -7.70 -4.13 -6.69 6.99 -2.00 -10.84 -9.17 -7.59 7.00
Nucleolus 7.16 3.15 1.83 -6.27 -1.66 -2.24 -1.72 -7.50 -5.01 -5.00 6.27 1.51 -0.09 -7.91 -1.74
Nucleus 8.06 -11.89 0.17 -5.82 10.93 -0.48 -5.83 -8.66 -9.70 1.57 8.32 -14.15 -3.33 -10.80 11.93
Peroxisomes 4.51 5.61 -0.61 -7.51 -9.18 -4.67 -2.76 -4.51 -0.72 -0.18 2.80 6.02 -0.85 -6.01 -11.79
Vacuole 4.41 -4.99 -11.00 -13.55 -6.31 -7.84 -10.78 -12.04 -5.74 8.49 -2.08 -10.18 -15.04 -13.27 -1.98
Vacuole Periphery -2.26 11.46 11.01 11.84 0.29 -5.30 10.87 9.63 14.22 -1.74 -4.40 13.91 14.16 18.42 0.47
Standard name
Human Ortholog
Description Protein involved in G2/M phase progression and response to DNA damage; interacts with Rad53p; contains an RNA recognition motif, a nuclear localization signal, and several SQ/TQ cluster domains; hyperphosphorylated in response to DNA damage
Localization
Cell Percentages cytoplasm (98%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-5

Pin4

Pin4


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Pin4-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available