Standard name
Human Ortholog
Description Mitochondrial peroxiredoxin with thioredoxin peroxidase activity; has a role in reduction of hydroperoxides; reactivation requires Trr2p and glutathione; induced during respiratory growth and oxidative stress; phosphorylated; protein abundance increases in response to DNA replication stress
DOWNLOAD

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – – – – –
Cell Periphery 0 0.12 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Cytoplasm 0 0 0 0.07 0 0 0.06 0 0.05 0 0.05 0 0 0 0.05 – – – 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Endosome 0.07 0 0.08 0 0.06 0.05 0 0 0 0 0 0 0 0.05 0 – – – 0.05 0 0.06 0 0 0
Golgi 0 0 0.24 0.1 0.18 0.05 0 0.12 0 0 0 0 0 0.16 0.33 – – – 0.05 0.05 0 0.1 0.14 0.11
Mitochondria 0.91 0.97 0.73 0.86 0.69 0.78 0.77 0.68 0.89 0.87 0.88 0.89 0.89 0.86 0.77 – – – 0.84 0.82 0.78 0.65 0.69 0.69
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0.05 0.13 0.07 0.06 0.05 0.06 0 0.09 0 0 0 – – – 0 0 0 0 0 0
Nucleolus 0.05 0 0 0 0.05 0.1 0.09 0.08 0.11 0.12 0.07 0.12 0 0 0 – – – 0 0 0 0 0 0
Peroxisomes 0 0 0.09 0 0 0 0 0 0 0 0 0 0 0.05 0.07 – – – 0 0 0.05 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Vac/Vac Membrane 0.18 0.11 0.09 0.06 0.18 0.11 0.09 0.12 0 0 0 0 0 0.11 0.07 – – – 0 0 0 0.05 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 1 0 0 0 1 1 3 0 2 0 2 0 – – – 0 2 1 3 5 5
Bud 0 2 3 3 2 2 7 3 7 6 3 2 0 2 1 – – – 0 0 1 0 2 2
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Bud Site 0 1 0 0 0 0 0 1 1 1 0 4 0 0 0 – – – – – – – – –
Cell Periphery 1 15 2 6 5 3 1 3 0 4 0 2 1 3 4 – – – 0 0 0 0 0 0
Cytoplasm 1 1 2 10 7 10 19 11 12 9 11 4 2 6 9 – – – 0 0 1 2 0 0
Endoplasmic Reticulum 0 1 4 0 0 2 0 1 0 0 0 0 0 0 3 – – – 0 0 0 1 0 0
Endosome 14 0 11 6 12 12 1 13 0 1 3 2 2 9 3 – – – 9 4 9 3 3 5
Golgi 6 2 33 15 37 14 7 42 1 6 2 5 2 27 56 – – – 9 7 6 8 26 21
Mitochondria 173 124 102 125 142 205 233 233 237 296 184 272 62 150 131 – – – 168 108 128 57 132 136
Nucleus 3 0 0 1 0 1 3 2 4 0 0 0 3 0 0 – – – 0 0 0 0 2 1
Nuclear Periphery 6 5 4 3 11 34 21 20 14 19 5 26 2 2 4 – – – 0 0 1 1 1 2
Nucleolus 9 1 2 2 10 27 27 27 30 39 15 36 3 3 1 – – – 0 0 0 0 3 4
Peroxisomes 1 1 13 5 9 4 7 7 1 4 0 4 2 9 12 – – – 5 5 8 1 3 6
SpindlePole 4 0 2 0 4 2 0 4 0 5 1 3 1 1 1 – – – 0 0 1 0 0 0
Vac/Vac Membrane 34 14 12 9 37 30 28 43 8 15 9 8 3 19 12 – – – 3 3 0 4 7 6
Unique Cell Count 191 128 140 145 206 264 302 345 265 339 209 305 70 174 170 202 133 165 89 192 198
Labelled Cell Count 252 167 190 186 276 346 354 411 316 408 233 370 83 233 237 202 133 165 89 192 198


Mitochondria

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 7.2 6.6 8.9 8.3 12.0 11.6 12.4 14.4 11.0 13.2 12.0 12.7 9.2 11.3 11.6 – – – 15.9 14.4 13.8
Std Deviation (1e-4) 1.7 1.2 1.9 1.8 3.5 2.9 3.6 4.7 4.1 4.7 5.0 5.7 4.4 6.3 5.0 – – – 16.0 13.7 12.5
Intensity Change (Log2) – – – -0.1 0.43 0.38 0.48 0.69 0.31 0.57 0.43 0.52 0.05 0.34 0.39 – – – 0.84 0.7 0.64


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 – – –
Bud 0 0 0 0 0 0 0 0 0 0 0 0 – – –
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 – – –
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 – – –
Cytoplasm 2.3 0 0 2.2 0 0 0 1.9 0 0 0 0 – – –
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 – – –
Endosome -1.3 -0.7 -1.4 0 -1.9 0 0 -3.0 0 0 -1.0 -2.6 – – –
Golgi -3.0 -1.3 -5.4 -7.2 -3.1 -8.0 -7.9 -6.9 -7.7 -3.8 -1.8 1.8 – – –
Mitochondria 2.8 -0.8 1.1 1.0 -1.1 4.3 3.8 3.6 4.4 2.6 3.0 0.9 – – –
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 – – –
Nuclear Periphery 0 1.1 3.3 1.7 1.4 1.1 1.3 0 2.2 0 0 0 – – –
Nucleolus 0 0 3.3 3.0 2.7 3.5 3.6 2.4 3.6 0 0 0 – – –
Peroxisomes -2.0 -1.8 -3.7 -3.3 -3.6 0 0 -4.5 -4.1 -1.7 -1.4 -0.7 – – –
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 – – –
Vacuole -0.8 2.5 0.9 0.2 1.2 -2.5 -1.8 -1.6 -2.8 -1.1 0.7 -0.5 – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 7.7578 8.0415 7.3111 7.9819 7.1009 8.0702 11.4241 11.7669 10.7165 9.414 9.809 11.322 8.2679 8.9635 8.2059 7.1154 6.6621 8.5131
Actin 0.022 0.018 0.0247 0.0001 0.0163 0.0024 0.0051 0.004 0.0025 0.0001 0.0001 0.0006 0.0167 0.0052 0.0164 0.0053 0.0261 0.0004
Bud 0.0004 0.0017 0.0004 0 0.0005 0.0001 0.0001 0.0001 0 0 0 0 0.0004 0.0001 0.0002 0.0002 0.0002 0
Bud Neck 0.0003 0.0002 0.0002 0 0.0001 0.0024 0 0 0 0 0 0.0001 0.0004 0.0001 0.0005 0.0005 0.0003 0
Bud Periphery 0.0007 0.0048 0.0004 0 0.0005 0.0001 0.0001 0.0001 0.0001 0 0.0001 0 0.001 0.0002 0.0003 0.0005 0.0004 0
Bud Site 0.0013 0.0047 0.0024 0 0.001 0.0002 0.0001 0.0003 0.0001 0 0 0 0.0043 0.0007 0.0017 0.004 0.0004 0
Cell Periphery 0.0002 0.0006 0.0001 0 0.0001 0.0001 0.0001 0 0 0 0 0 0.0002 0.0001 0.0001 0.0003 0.0002 0
Cytoplasm 0.0007 0.0001 0.0047 0 0.0001 0.0001 0 0 0 0 0 0 0.0013 0 0.0029 0.0008 0.0007 0.0001
Cytoplasmic Foci 0.0116 0.0018 0.0172 0.0001 0.0065 0.0016 0.0012 0.001 0.0019 0 0.0002 0.0087 0.0192 0.003 0.0071 0.0039 0.0017 0.0005
Eisosomes 0.0003 0.0003 0.0008 0 0.0001 0.0001 0.0003 0.0001 0.0001 0 0.0001 0.0001 0.0002 0.0001 0.0002 0.0005 0.0003 0.0001
Endoplasmic Reticulum 0.0008 0.0001 0.0052 0 0.0001 0 0 0 0 0 0 0 0.0013 0.0001 0.0013 0.0049 0.0046 0.0003
Endosome 0.0117 0.0079 0.0241 0.0001 0.0118 0.0041 0.0027 0.0011 0.0032 0.0001 0.0007 0.0006 0.0376 0.0037 0.0108 0.0113 0.0168 0.0116
Golgi 0.0132 0.0174 0.0091 0.0011 0.0323 0.0071 0.005 0.0041 0.0146 0.0001 0.001 0.0019 0.0245 0.0101 0.004 0.0091 0.0065 0.0057
Lipid Particles 0.03 0.0038 0.0164 0.0001 0.0052 0.0021 0.0143 0.0006 0.0081 0.0004 0.0005 0.0061 0.0248 0.0121 0.0095 0.0153 0.011 0.0192
Mitochondria 0.8658 0.9172 0.8572 0.9966 0.9066 0.976 0.9542 0.9773 0.9586 0.9977 0.9943 0.9713 0.8264 0.927 0.9076 0.9234 0.9059 0.9518
None 0.0004 0.0001 0.001 0 0 0 0 0 0 0 0 0 0.001 0 0.0129 0.0055 0.0009 0
Nuclear Periphery 0.0028 0.0001 0.0027 0 0.0002 0 0 0 0 0 0 0.0002 0.0099 0.0003 0.0025 0.0051 0.008 0.0013
Nucleolus 0.0003 0.0025 0.0005 0 0 0.0001 0.0001 0 0 0 0 0 0.0005 0.0002 0.0003 0.0007 0.0005 0.0002
Nucleus 0.0111 0.0005 0.0006 0 0.0001 0.0001 0 0 0 0 0 0 0.0076 0.0001 0.0007 0.0007 0.0017 0.0001
Peroxisomes 0.021 0.0086 0.0121 0.0014 0.0074 0.0021 0.014 0.0105 0.0097 0.0012 0.0024 0.0022 0.0083 0.0141 0.0093 0.0039 0.0021 0.0035
Punctate Nuclear 0.001 0 0.0047 0 0.0001 0.0001 0 0 0 0 0 0.0001 0.0042 0 0.0012 0.0005 0.0013 0
Vacuole 0.0016 0.0061 0.0027 0.0001 0.0013 0.0006 0.0006 0.0001 0.0002 0.0001 0.0001 0.0003 0.0038 0.003 0.0019 0.002 0.004 0.0015
Vacuole Periphery 0.0029 0.0035 0.0126 0.0003 0.0097 0.0006 0.002 0.0004 0.0007 0.0002 0.0003 0.0077 0.0063 0.0197 0.0089 0.0017 0.0063 0.0036

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 42.1147 35.1381 54.0104 66.7811 40.0426 64.5401 49.4855 65.086 76.4423 57.9161
Translational Efficiency 1.5313 1.8256 1.6063 1.6995 1.956 1.5093 1.4783 1.7234 1.8438 1.6397

Hit Data

Dataset Hit
Protein Concentration ✔
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Mitochondrial peroxiredoxin with thioredoxin peroxidase activity; has a role in reduction of hydroperoxides; reactivation requires Trr2p and glutathione; induced during respiratory growth and oxidative stress; phosphorylated; protein abundance increases in response to DNA replication stress
Localization
Cell Percentages mitochondrion (96%)
Cell Cycle Regulation No
Subcompartmental Group mito-2

Prx1

Prx1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Prx1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available