Standard name
Human Ortholog
Description Ribosomal 60S subunit protein L23A; homologous to mammalian ribosomal protein L23 and bacterial L14; RPL23A has a paralog, RPL23B, that arose from the whole genome duplication
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.05 0 0 0 0.05 0.2 0.2 0.18 0.24 0.16 0.13 0.17 0.05 0.06 0.16 0 0.05 0.06 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0.11 0.07 0.06 0.06 0.33 0.23 0.29 0.38 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0.94 0.95 0.94 0.94 0.87 0.64 0.71 0.73 0.55 0.65 0.64 0.59 0.93 0.9 0.78 0.8 0.81 0.76 0.94 0.94 0.94 0.68 0.59 0.46
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0.06 0.06 0 0 0 0.13 0.09 0.11 0 0 0 0.07 0.12 0.1
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.09
Vac/Vac Membrane 0 0 0 0 0 0.1 0 0 0 0 0.09 0 0 0 0.06 0 0 0.08 0 0 0 0.19 0.19 0.26
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 1 4 5 1 1 0 0 0 0 0 0 5 2 0 3 5 4
Bud Neck 0 0 0 0 0 1 0 0 0 2 1 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 1 0 1 1 6 8 2 8 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0
Cytoplasm 19 12 12 9 10 58 56 55 70 56 34 35 18 23 78 14 15 15 1 3 0 1 3 0
Endoplasmic Reticulum 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 1 1 1 0 0 0 0 0 0
Endosome 1 1 0 0 0 6 3 3 2 2 3 0 1 4 7 11 10 5 0 1 0 1 5 9
Golgi 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 1 0 1 0 0 0 0 1 2
Mitochondria 0 1 0 0 20 20 18 17 96 78 78 80 0 0 0 7 4 1 0 2 0 1 3 5
Nucleus 344 528 257 198 164 185 204 221 162 224 171 124 347 326 380 294 269 201 363 549 269 224 206 174
Nuclear Periphery 0 2 1 1 0 1 2 0 1 4 2 2 0 0 1 2 0 0 0 0 0 1 1 2
Nucleolus 5 7 4 2 5 2 2 7 13 11 16 12 3 3 4 46 30 28 5 1 4 24 41 38
Peroxisomes 0 0 0 0 0 1 0 0 1 0 0 0 0 0 2 4 3 4 0 0 0 0 1 2
SpindlePole 0 0 0 0 0 3 3 4 2 1 2 3 0 2 3 12 10 10 0 0 0 3 7 32
Vac/Vac Membrane 9 11 7 2 7 29 11 13 10 10 25 8 4 11 27 10 11 20 8 15 5 63 66 96
Unique Cell Count 367 555 274 211 189 287 286 301 293 345 267 211 372 363 490 366 331 263 387 584 285 331 349 378
Labelled Cell Count 378 562 281 212 209 306 300 322 367 401 335 273 374 369 502 402 354 286 387 584 285 331 349 378


Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 27.5 21.1 25.8 18.2 5.7 5.4 5.2 5.7 4.4 4.9 5.2 4.6 25.5 16.1 10.6 16.5 24.3 22.6 23.6 18.7 17.1
Std Deviation (1e-4) 12.5 7.0 10.2 6.8 1.7 1.9 1.5 1.6 1.9 1.9 2.0 1.9 8.0 5.1 3.3 5.7 9.0 8.3 8.9 6.9 5.2
Intensity Change (Log2) – – – -0.51 -2.18 -2.25 -2.31 -2.17 -2.55 -2.39 -2.32 -2.49 -0.02 -0.68 -1.29 -0.64 -0.09 -0.2 -0.13 -0.46 -0.6


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm -0.1 0.5 5.7 5.5 5.2 6.6 4.7 3.5 4.5 0.3 1.1 4.7 -0.4 0.1 0.7
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 5.5 4.4 4.2 4.0 0 8.4 9.7 0 0 0 0 0 0 0
Nucleus 0 -2.6 -8.5 -7.0 -6.5 -10.4 -8.6 -8.5 -9.3 -0.3 -1.8 -5.8 -4.9 -4.6 -5.7
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0.8 0 1.4 2.8 0 0 0 0 5.2 4.0 4.5
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 3.0 0 0
Vacuole 0 0.7 3.6 0.9 1.2 0.6 0.3 3.4 0.8 0 0.4 1.9 0.1 0.6 2.7

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 32.1344 34.5152 32.084 31.5021 26.0511 29.5654 38.6139 40.7218 37.8055 39.3457 37.9576 39.3434 36.4776 38.0635 35.5433 35.8239 31.2706 35.1796
Actin 0.0029 0 0.0062 0 0.0004 0 0.0034 0 0.0053 0.0062 0.0035 0.0001 0.0027 0 0.001 0.0001 0.0001 0
Bud 0.0001 0 0.0001 0 0.0001 0.0002 0.0001 0 0.0004 0.0006 0.0001 0.0001 0.0002 0 0.0002 0.0002 0.0002 0
Bud Neck 0.0002 0 0.0001 0 0.0001 0.0006 0.0001 0 0.0002 0.0003 0.0001 0.0002 0.0001 0 0 0.0001 0.0001 0.0003
Bud Periphery 0.0001 0 0.0001 0 0.0001 0.0004 0.0001 0 0.0009 0.0009 0.0002 0.0002 0.0002 0 0.0001 0.0002 0.0001 0.0001
Bud Site 0.001 0 0.0001 0 0.0002 0.0002 0.0002 0 0.0003 0.0015 0.0003 0 0.0002 0 0.0002 0.0002 0.0003 0
Cell Periphery 0.0003 0.0001 0.0001 0 0.0012 0.0005 0.0002 0 0.0002 0.0001 0.0001 0.0002 0.0005 0 0.0003 0.0002 0.0009 0.0002
Cytoplasm 0.0047 0 0.0005 0 0.0027 0.0005 0.0014 0 0.0017 0.0005 0.0003 0.0002 0.0038 0.0001 0.0029 0.0021 0.0067 0
Cytoplasmic Foci 0.0106 0 0.0052 0 0.033 0.0016 0.0037 0 0.0015 0.0011 0.0002 0 0.0098 0 0.0047 0.0125 0.0134 0
Eisosomes 0.0002 0 0.0001 0 0 0 0.0001 0 0.0001 0.0001 0.0002 0 0 0 0 0 0.0003 0
Endoplasmic Reticulum 0.0002 0 0.0007 0 0.0001 0 0.0009 0 0.003 0.0007 0.0002 0.0001 0.0031 0 0.0004 0.0009 0.0003 0
Endosome 0.0014 0 0.0087 0 0.0038 0.0002 0.0049 0 0.0135 0.0048 0.0008 0.0001 0.0049 0 0.006 0.0026 0.0003 0
Golgi 0.0008 0 0.0041 0 0.0026 0 0.0022 0 0.0037 0.0017 0.0002 0 0.0005 0 0.0022 0.0002 0.0001 0
Lipid Particles 0.0032 0 0.0019 0 0.0149 0.0014 0.0019 0 0.0015 0.0015 0.0003 0 0.0023 0 0.0028 0.0069 0.003 0
Mitochondria 0.0049 0.0003 0.0016 0.0003 0.0139 0.0047 0.0029 0.0007 0.005 0.0085 0.011 0.0008 0.0019 0.0004 0.0038 0.0015 0.0035 0.0003
None 0.0093 0 0.0002 0 0.0007 0.0002 0.0126 0 0.0007 0.001 0.0014 0.0001 0.0019 0 0.0019 0.0169 0.0517 0
Nuclear Periphery 0.0008 0.0015 0.0019 0.0007 0.0064 0.0008 0.0053 0.003 0.007 0.0219 0.0127 0.0039 0.0101 0.0029 0.0021 0.0252 0.0031 0.001
Nucleolus 0.0548 0.0181 0.0229 0.0153 0.1018 0.0737 0.0873 0.0225 0.0608 0.0191 0.0827 0.0698 0.0777 0.0248 0.0713 0.0302 0.1406 0.0573
Nucleus 0.8964 0.9785 0.9406 0.9834 0.7442 0.9104 0.8651 0.9728 0.8897 0.9127 0.8799 0.9221 0.8689 0.9712 0.8938 0.881 0.758 0.9393
Peroxisomes 0.0018 0 0.0014 0 0.0314 0.0005 0.0004 0 0.0002 0.0005 0.0003 0 0.0002 0 0.0004 0.0003 0.0002 0
Punctate Nuclear 0.004 0 0.0024 0 0.0328 0.0002 0.0049 0 0.0005 0.0138 0.0035 0.0005 0.0041 0.0001 0.0011 0.0087 0.0118 0
Vacuole 0.0011 0.0004 0.0005 0 0.003 0.0023 0.001 0.0001 0.0015 0.0009 0.0005 0.0007 0.0037 0.0002 0.0016 0.004 0.0024 0.0008
Vacuole Periphery 0.0013 0.001 0.0009 0.0001 0.0064 0.0015 0.0014 0.0008 0.0024 0.0016 0.0014 0.001 0.0029 0.0003 0.0031 0.0058 0.003 0.0005

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 1649.7762 1653.4905 2202.8729 1780.6493 1945.4942 1347.8588 1462.8622 1436.4908 1563.7507 1495.0432
Translational Efficiency 1.7361 1.9416 1.5165 2.0788 1.7955 1.9072 1.927 2.0853 1.6045 1.6825

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1275 1313 3202 1746 1818 2392 2617 175 3093 3705 5819 1921

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 2049.31 2199.57 3527.07 3838.33 1622.29 2270.26 3713.56 4056.14 1798.32 2245.21 3610.94 3858.17
Standard Deviation 518.78 653.32 986.88 1111.20 401.09 549.49 935.51 1128.71 499.68 589.36 968.57 1114.57
Intensity Change Log 2 — 0.102083 0.783332 0.905341 — 0.484826 1.194771 1.322076 — 0.283811 0.979706 1.104434

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000374 0.000601 0.000358 0.001559 0.000436 0.000501 0.000545 0.001121 0.000410 0.000537 0.000442 0.001519
Bud Neck 0.002701 0.002834 0.004814 0.019154 0.004129 0.006146 0.008268 0.021301 0.003540 0.004972 0.006368 0.019350
Bud Site 0.000133 0.000414 0.000336 0.003339 0.000303 0.000699 0.000771 0.003666 0.000233 0.000598 0.000532 0.003369
Cell Periphery 0.000794 0.000312 0.000194 0.000213 0.000163 0.000458 0.000133 0.000090 0.000423 0.000406 0.000167 0.000202
Cytoplasm 0.000083 0.000275 0.000288 0.001144 0.000202 0.000207 0.000292 0.000474 0.000153 0.000231 0.000290 0.001083
Cytoplasmic Foci 0.000007 0.000319 0.000028 0.009975 0.000020 0.000123 0.000183 0.023322 0.000015 0.000193 0.000098 0.011191
Eisosomes 0.000213 0.000147 0.000082 0.000075 0.000145 0.000214 0.000085 0.000091 0.000173 0.000190 0.000084 0.000076
Endoplasmic Reticulum 0.003846 0.000555 0.000672 0.000329 0.001683 0.001523 0.000546 0.000230 0.002575 0.001180 0.000615 0.000320
Endosome 0.000027 0.000057 0.000011 0.000794 0.000036 0.000103 0.000016 0.000309 0.000033 0.000087 0.000013 0.000750
Golgi 0.000006 0.000102 0.000012 0.001058 0.000027 0.000054 0.000027 0.000524 0.000018 0.000071 0.000018 0.001009
Lipid Particles 0.000001 0.000207 0.000001 0.000114 0.000007 0.000014 0.000004 0.000905 0.000004 0.000083 0.000002 0.000186
Mitochondria 0.000466 0.002012 0.000757 0.005499 0.000772 0.001026 0.001315 0.005344 0.000646 0.001375 0.001008 0.005485
Mitotic Spindle 0.000024 0.000626 0.000026 0.033384 0.000062 0.000376 0.000572 0.048659 0.000046 0.000465 0.000271 0.034776
None 0.000744 0.001797 0.001207 0.002066 0.000466 0.000985 0.000622 0.001300 0.000580 0.001273 0.000944 0.001996
Nuclear Periphery 0.000158 0.000065 0.000098 0.000780 0.000067 0.000141 0.000116 0.000649 0.000105 0.000114 0.000106 0.000768
Nuclear Periphery Foci 0.000011 0.000125 0.000002 0.000436 0.000126 0.000160 0.000016 0.000742 0.000078 0.000148 0.000008 0.000464
Nucleolus 0.002656 0.014683 0.002643 0.164795 0.009880 0.008859 0.016181 0.164454 0.006902 0.010923 0.008731 0.164764
Nucleus 0.985349* 0.972741* 0.987882* 0.732229* 0.980057* 0.975922* 0.968859* 0.695246* 0.982239* 0.974795* 0.979327* 0.728860*
Peroxisomes 0.000052 0.001143 0.000119 0.021001 0.000373 0.000332 0.000778 0.029865 0.000241 0.000620 0.000415 0.021809
Vacuole 0.002145 0.000760 0.000395 0.001067 0.000841 0.001666 0.000534 0.001153 0.001378 0.001345 0.000457 0.001075
Vacuole Periphery 0.000211 0.000227 0.000075 0.000989 0.000202 0.000490 0.000137 0.000555 0.000206 0.000397 0.000103 0.000949

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -3.46 -0.12 -11.33 -7.68 -11.38 -2.86 -7.28 -6.72 -6.12 -5.13 -4.60 -5.13 -11.77 -10.04 -10.93
Bud Neck -0.63 -8.12 -16.66 -16.61 -14.81 -5.14 -10.74 -6.11 -5.54 -4.06 -5.39 -12.67 -17.21 -16.05 -13.64
Bud Site -1.67 -5.84 -15.99 -12.63 -15.14 -2.92 -5.03 -6.96 -6.42 -5.70 -3.44 -5.96 -16.98 -14.65 -14.42
Cell Periphery 1.47 2.91 2.78 0.80 -0.65 -3.33 0.92 0.76 3.68 -0.21 -0.06 2.93 2.39 2.40 -1.41
Cytoplasm -2.37 -6.87 -7.73 -5.26 -6.14 -0.03 -1.10 -2.09 -3.72 -1.55 -0.95 -2.00 -6.27 -6.44 -5.86
Cytoplasmic Foci -2.62 -4.32 -10.39 -10.14 -10.38 -2.08 -4.62 -4.69 -4.67 -4.60 -3.34 -4.78 -11.33 -11.19 -11.12
Eisosomes 2.33 4.91 4.71 4.89 -1.20 -1.95 4.42 1.55 2.66 -2.72 -0.65 6.64 6.23 4.45 -1.58
Endoplasmic Reticulum 10.10 9.88 10.90 3.81 7.87 0.97 9.28 10.54 8.93 2.94 8.17 12.93 14.78 10.09 8.04
Endosome -0.99 2.77 -2.36 -2.18 -2.42 -2.45 1.00 -1.42 -0.96 -1.52 -2.65 1.96 -2.43 -2.13 -2.50
Golgi -1.43 -3.72 -4.41 -3.79 -4.38 -0.87 -1.89 -8.45 -7.83 -8.23 -1.67 -1.57 -4.76 -4.42 -4.68
Lipid Particles -1.39 -1.36 -4.96 0.60 -4.94 -1.38 -0.17 -1.32 -1.30 -1.32 -1.51 0.20 -3.21 -1.14 -3.22
Mitochondria -2.55 -8.08 -20.40 -5.34 -19.09* -3.93 -10.35 -5.25 -4.92 -4.43 -3.40 -11.89 -20.41 -12.73 -18.40
Mitotic Spindle -2.05 -0.29 -12.68 -12.45 -12.68 -1.98 -3.90 -5.16 -5.13 -5.03 -2.83 -3.86 -13.66 -13.49 -13.39
None -1.56 -1.07 -3.64 -0.38 -2.52 -2.18 -2.94 -6.70 -1.96 -5.15 -2.37 -1.91 -7.13 -2.39 -4.65
Nuclear Periphery 5.59 3.73 -13.19 -15.18 -14.61 -4.09 -6.86 -5.21 -4.58 -4.63 -1.10 -2.08 -15.34 -14.61 -14.99
Nuclear Periphery Foci -1.49 0.98 -8.65 -3.82 -8.84 -0.60 0.90 -1.77 -1.25 -2.14 -0.96 1.02 -5.36 -2.30 -8.56
Nucleolus -7.64 -1.04 -34.16* -31.54* -34.09* 0.56 -8.52 -11.40 -11.44 -10.24 -4.56 -7.29 -35.38* -34.58* -33.55*
Nucleus 4.90 -0.67 39.81 37.11 40.08 2.69 10.63 14.71 14.52 13.44 5.38 8.63 42.13 40.82 40.03
Peroxisomes -3.72 -3.75 -19.83 -18.62 -19.76* 0.31 -5.74 -7.99 -7.99 -7.72 -3.47 -5.71 -21.20 -20.84 -20.64
Vacuole 3.64 5.69 2.56 -1.42 -4.15 -2.29 0.70 -2.42 -0.15 -2.66 0.03 5.15 0.51 0.37 -3.71
Vacuole Periphery -0.84 8.97 -3.84 -3.74 -4.55 -2.23 1.04 -7.74 -1.62 -8.79 -2.31 4.77 -4.12 -2.75 -4.63
Standard name
Human Ortholog
Description Ribosomal 60S subunit protein L23A; homologous to mammalian ribosomal protein L23 and bacterial L14; RPL23A has a paralog, RPL23B, that arose from the whole genome duplication
Localization
Cell Percentages nucleus (97%)
Cell Cycle Regulation No
Subcompartmental Group nucleus-2

Rpl23a

Rpl23a


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Rpl23a-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available