Standard name | |
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Human Ortholog | |
Description | Ribosomal 60S subunit protein L23A; homologous to mammalian ribosomal protein L23 and bacterial L14; RPL23A has a paralog, RPL23B, that arose from the whole genome duplication |
ensLOC | DeepLoc | |||||||||||||||||||||||
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Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 4 | 5 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 5 | 2 | 0 | 3 | 5 | 4 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 2 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 1 | 6 | 8 | 2 | 8 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 19 | 12 | 12 | 9 | 10 | 58 | 56 | 55 | 70 | 56 | 34 | 35 | 18 | 23 | 78 | 14 | 15 | 15 | 1 | 3 | 0 | 1 | 3 | 0 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 1 | 1 | 0 | 0 | 0 | 6 | 3 | 3 | 2 | 2 | 3 | 0 | 1 | 4 | 7 | 11 | 10 | 5 | 0 | 1 | 0 | 1 | 5 | 9 |
Golgi | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 1 | 2 |
Mitochondria | 0 | 1 | 0 | 0 | 20 | 20 | 18 | 17 | 96 | 78 | 78 | 80 | 0 | 0 | 0 | 7 | 4 | 1 | 0 | 2 | 0 | 1 | 3 | 5 |
Nucleus | 344 | 528 | 257 | 198 | 164 | 185 | 204 | 221 | 162 | 224 | 171 | 124 | 347 | 326 | 380 | 294 | 269 | 201 | 363 | 549 | 269 | 224 | 206 | 174 |
Nuclear Periphery | 0 | 2 | 1 | 1 | 0 | 1 | 2 | 0 | 1 | 4 | 2 | 2 | 0 | 0 | 1 | 2 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 2 |
Nucleolus | 5 | 7 | 4 | 2 | 5 | 2 | 2 | 7 | 13 | 11 | 16 | 12 | 3 | 3 | 4 | 46 | 30 | 28 | 5 | 1 | 4 | 24 | 41 | 38 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 2 | 4 | 3 | 4 | 0 | 0 | 0 | 0 | 1 | 2 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 3 | 3 | 4 | 2 | 1 | 2 | 3 | 0 | 2 | 3 | 12 | 10 | 10 | 0 | 0 | 0 | 3 | 7 | 32 |
Vac/Vac Membrane | 9 | 11 | 7 | 2 | 7 | 29 | 11 | 13 | 10 | 10 | 25 | 8 | 4 | 11 | 27 | 10 | 11 | 20 | 8 | 15 | 5 | 63 | 66 | 96 |
Unique Cell Count | 367 | 555 | 274 | 211 | 189 | 287 | 286 | 301 | 293 | 345 | 267 | 211 | 372 | 363 | 490 | 366 | 331 | 263 | 387 | 584 | 285 | 331 | 349 | 378 |
Labelled Cell Count | 378 | 562 | 281 | 212 | 209 | 306 | 300 | 322 | 367 | 401 | 335 | 273 | 374 | 369 | 502 | 402 | 354 | 286 | 387 | 584 | 285 | 331 | 349 | 378 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 27.5 | 21.1 | 25.8 | 18.2 | 5.7 | 5.4 | 5.2 | 5.7 | 4.4 | 4.9 | 5.2 | 4.6 | 25.5 | 16.1 | 10.6 | 16.5 | 24.3 | 22.6 | 23.6 | 18.7 | 17.1 |
Std Deviation (1e-4) | 12.5 | 7.0 | 10.2 | 6.8 | 1.7 | 1.9 | 1.5 | 1.6 | 1.9 | 1.9 | 2.0 | 1.9 | 8.0 | 5.1 | 3.3 | 5.7 | 9.0 | 8.3 | 8.9 | 6.9 | 5.2 |
Intensity Change (Log2) | – | – | – | -0.51 | -2.18 | -2.25 | -2.31 | -2.17 | -2.55 | -2.39 | -2.32 | -2.49 | -0.02 | -0.68 | -1.29 | -0.64 | -0.09 | -0.2 | -0.13 | -0.46 | -0.6 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | -0.1 | 0.5 | 5.7 | 5.5 | 5.2 | 6.6 | 4.7 | 3.5 | 4.5 | 0.3 | 1.1 | 4.7 | -0.4 | 0.1 | 0.7 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 5.5 | 4.4 | 4.2 | 4.0 | 0 | 8.4 | 9.7 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 0 | -2.6 | -8.5 | -7.0 | -6.5 | -10.4 | -8.6 | -8.5 | -9.3 | -0.3 | -1.8 | -5.8 | -4.9 | -4.6 | -5.7 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0.8 | 0 | 1.4 | 2.8 | 0 | 0 | 0 | 0 | 5.2 | 4.0 | 4.5 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3.0 | 0 | 0 |
Vacuole | 0 | 0.7 | 3.6 | 0.9 | 1.2 | 0.6 | 0.3 | 3.4 | 0.8 | 0 | 0.4 | 1.9 | 0.1 | 0.6 | 2.7 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 32.1344 | 34.5152 | 32.084 | 31.5021 | 26.0511 | 29.5654 | 38.6139 | 40.7218 | 37.8055 | 39.3457 | 37.9576 | 39.3434 | 36.4776 | 38.0635 | 35.5433 | 35.8239 | 31.2706 | 35.1796 |
Actin | 0.0029 | 0 | 0.0062 | 0 | 0.0004 | 0 | 0.0034 | 0 | 0.0053 | 0.0062 | 0.0035 | 0.0001 | 0.0027 | 0 | 0.001 | 0.0001 | 0.0001 | 0 |
Bud | 0.0001 | 0 | 0.0001 | 0 | 0.0001 | 0.0002 | 0.0001 | 0 | 0.0004 | 0.0006 | 0.0001 | 0.0001 | 0.0002 | 0 | 0.0002 | 0.0002 | 0.0002 | 0 |
Bud Neck | 0.0002 | 0 | 0.0001 | 0 | 0.0001 | 0.0006 | 0.0001 | 0 | 0.0002 | 0.0003 | 0.0001 | 0.0002 | 0.0001 | 0 | 0 | 0.0001 | 0.0001 | 0.0003 |
Bud Periphery | 0.0001 | 0 | 0.0001 | 0 | 0.0001 | 0.0004 | 0.0001 | 0 | 0.0009 | 0.0009 | 0.0002 | 0.0002 | 0.0002 | 0 | 0.0001 | 0.0002 | 0.0001 | 0.0001 |
Bud Site | 0.001 | 0 | 0.0001 | 0 | 0.0002 | 0.0002 | 0.0002 | 0 | 0.0003 | 0.0015 | 0.0003 | 0 | 0.0002 | 0 | 0.0002 | 0.0002 | 0.0003 | 0 |
Cell Periphery | 0.0003 | 0.0001 | 0.0001 | 0 | 0.0012 | 0.0005 | 0.0002 | 0 | 0.0002 | 0.0001 | 0.0001 | 0.0002 | 0.0005 | 0 | 0.0003 | 0.0002 | 0.0009 | 0.0002 |
Cytoplasm | 0.0047 | 0 | 0.0005 | 0 | 0.0027 | 0.0005 | 0.0014 | 0 | 0.0017 | 0.0005 | 0.0003 | 0.0002 | 0.0038 | 0.0001 | 0.0029 | 0.0021 | 0.0067 | 0 |
Cytoplasmic Foci | 0.0106 | 0 | 0.0052 | 0 | 0.033 | 0.0016 | 0.0037 | 0 | 0.0015 | 0.0011 | 0.0002 | 0 | 0.0098 | 0 | 0.0047 | 0.0125 | 0.0134 | 0 |
Eisosomes | 0.0002 | 0 | 0.0001 | 0 | 0 | 0 | 0.0001 | 0 | 0.0001 | 0.0001 | 0.0002 | 0 | 0 | 0 | 0 | 0 | 0.0003 | 0 |
Endoplasmic Reticulum | 0.0002 | 0 | 0.0007 | 0 | 0.0001 | 0 | 0.0009 | 0 | 0.003 | 0.0007 | 0.0002 | 0.0001 | 0.0031 | 0 | 0.0004 | 0.0009 | 0.0003 | 0 |
Endosome | 0.0014 | 0 | 0.0087 | 0 | 0.0038 | 0.0002 | 0.0049 | 0 | 0.0135 | 0.0048 | 0.0008 | 0.0001 | 0.0049 | 0 | 0.006 | 0.0026 | 0.0003 | 0 |
Golgi | 0.0008 | 0 | 0.0041 | 0 | 0.0026 | 0 | 0.0022 | 0 | 0.0037 | 0.0017 | 0.0002 | 0 | 0.0005 | 0 | 0.0022 | 0.0002 | 0.0001 | 0 |
Lipid Particles | 0.0032 | 0 | 0.0019 | 0 | 0.0149 | 0.0014 | 0.0019 | 0 | 0.0015 | 0.0015 | 0.0003 | 0 | 0.0023 | 0 | 0.0028 | 0.0069 | 0.003 | 0 |
Mitochondria | 0.0049 | 0.0003 | 0.0016 | 0.0003 | 0.0139 | 0.0047 | 0.0029 | 0.0007 | 0.005 | 0.0085 | 0.011 | 0.0008 | 0.0019 | 0.0004 | 0.0038 | 0.0015 | 0.0035 | 0.0003 |
None | 0.0093 | 0 | 0.0002 | 0 | 0.0007 | 0.0002 | 0.0126 | 0 | 0.0007 | 0.001 | 0.0014 | 0.0001 | 0.0019 | 0 | 0.0019 | 0.0169 | 0.0517 | 0 |
Nuclear Periphery | 0.0008 | 0.0015 | 0.0019 | 0.0007 | 0.0064 | 0.0008 | 0.0053 | 0.003 | 0.007 | 0.0219 | 0.0127 | 0.0039 | 0.0101 | 0.0029 | 0.0021 | 0.0252 | 0.0031 | 0.001 |
Nucleolus | 0.0548 | 0.0181 | 0.0229 | 0.0153 | 0.1018 | 0.0737 | 0.0873 | 0.0225 | 0.0608 | 0.0191 | 0.0827 | 0.0698 | 0.0777 | 0.0248 | 0.0713 | 0.0302 | 0.1406 | 0.0573 |
Nucleus | 0.8964 | 0.9785 | 0.9406 | 0.9834 | 0.7442 | 0.9104 | 0.8651 | 0.9728 | 0.8897 | 0.9127 | 0.8799 | 0.9221 | 0.8689 | 0.9712 | 0.8938 | 0.881 | 0.758 | 0.9393 |
Peroxisomes | 0.0018 | 0 | 0.0014 | 0 | 0.0314 | 0.0005 | 0.0004 | 0 | 0.0002 | 0.0005 | 0.0003 | 0 | 0.0002 | 0 | 0.0004 | 0.0003 | 0.0002 | 0 |
Punctate Nuclear | 0.004 | 0 | 0.0024 | 0 | 0.0328 | 0.0002 | 0.0049 | 0 | 0.0005 | 0.0138 | 0.0035 | 0.0005 | 0.0041 | 0.0001 | 0.0011 | 0.0087 | 0.0118 | 0 |
Vacuole | 0.0011 | 0.0004 | 0.0005 | 0 | 0.003 | 0.0023 | 0.001 | 0.0001 | 0.0015 | 0.0009 | 0.0005 | 0.0007 | 0.0037 | 0.0002 | 0.0016 | 0.004 | 0.0024 | 0.0008 |
Vacuole Periphery | 0.0013 | 0.001 | 0.0009 | 0.0001 | 0.0064 | 0.0015 | 0.0014 | 0.0008 | 0.0024 | 0.0016 | 0.0014 | 0.001 | 0.0029 | 0.0003 | 0.0031 | 0.0058 | 0.003 | 0.0005 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 1649.7762 | 1653.4905 | 2202.8729 | 1780.6493 | 1945.4942 | 1347.8588 | 1462.8622 | 1436.4908 | 1563.7507 | 1495.0432 |
Translational Efficiency | 1.7361 | 1.9416 | 1.5165 | 2.0788 | 1.7955 | 1.9072 | 1.927 | 2.0853 | 1.6045 | 1.6825 |
Dataset | Hit |
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Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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