Standard name
Human Ortholog
Description Histone H3; core histone protein required for chromatin assembly, part of heterochromatin-mediated telomeric and HM silencing; one of two identical histone H3 proteins (see HHT2); regulated by acetylation, methylation, and phosphorylation; H3K14 acetylation plays an important role in the unfolding of strongly positioned nucleosomes during repair of UV damage
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 – 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0.1 0 0 0 0 0 0 0 0
Cytoplasm 0.98 0.97 0.93 0.95 0.92 0.81 – 0.7 0.64 0.68 0.53 0.52 1.0 0.99 0.99 1.0 0.95 0.88 0.88 0.83 0.64 0.84 0.82 0.79
Endoplasmic Reticulum 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0.1 0 0.07 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0.05 0.05 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0.13 0.24 0.12 0.39 – 0.39 0.56 0.52 0.64 0.65 0 0 0 0.05 0 0 0.05 0.09 0.14 0 0.08 0.06
Nucleus 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 – 0 0 0 0.06 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0.09 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 1 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 3 1 5 0 2 0
Bud 0 1 3 5 1 5 – 23 6 16 16 11 0 0 0 0 0 0 0 1 3 2 4 9
Bud Neck 2 0 2 0 0 0 – 8 0 1 3 1 0 0 0 0 0 0 2 0 1 0 1 2
Bud Site 0 0 0 0 0 1 – 1 4 2 2 7 0 0 0 0 0 0 – – – – – –
Cell Periphery 2 0 2 3 1 0 – 6 0 2 1 2 0 1 0 2 0 0 1 0 3 1 0 0
Cytoplasm 354 187 185 228 281 295 – 440 227 326 157 218 211 278 348 20 36 38 305 160 126 130 205 207
Endoplasmic Reticulum 0 1 1 0 1 1 – 2 1 0 1 0 0 0 0 2 1 3 0 1 2 0 1 0
Endosome 0 1 0 0 7 1 – 8 0 1 0 3 0 1 1 0 2 2 1 1 4 1 0 4
Golgi 0 0 0 0 1 0 – 0 0 0 0 0 0 0 0 0 0 1 1 0 1 1 1 1
Mitochondria 8 3 25 58 35 141 – 247 198 251 189 274 1 1 0 1 0 1 17 17 26 6 19 14
Nucleus 0 0 0 1 2 1 – 4 1 2 2 3 0 1 0 0 0 1 0 0 0 0 0 2
Nuclear Periphery 0 0 0 1 0 0 – 0 1 1 0 0 0 0 0 0 0 0 0 0 1 0 0 1
Nucleolus 0 0 0 0 1 0 – 3 1 1 3 1 0 1 1 0 0 0 0 0 0 0 0 1
Peroxisomes 1 0 0 0 2 0 – 2 0 1 0 2 0 0 1 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 2 0 0 4 – 20 5 11 17 18 0 0 1 0 0 0 5 2 4 1 0 2
Vac/Vac Membrane 0 1 1 1 1 6 – 7 6 7 4 3 0 1 0 0 0 4 2 3 4 4 6 6
Unique Cell Count 360 193 199 239 304 365 628 352 480 294 419 212 282 350 20 38 43 348 193 197 156 250 263
Labelled Cell Count 367 195 221 297 333 455 771 450 622 395 543 212 284 352 25 39 50 348 193 197 156 250 263


Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 3.4 4.0 3.2 3.2 4.2 3.1 – 3.1 2.6 3.0 2.8 2.6 4.5 4.5 4.6 5.0 5.5 5.7 4.3 4.6 4.8
Std Deviation (1e-4) 0.4 0.6 1.6 1.9 2.7 2.0 – 1.9 1.5 1.8 1.9 1.2 2.3 2.2 2.3 0.6 1.9 1.9 2.2 2.3 2.3
Intensity Change (Log2) – – – 0.01 0.41 -0.05 – -0.02 -0.28 -0.08 -0.17 -0.3 0.51 0.49 0.52 0.66 0.79 0.84 0.43 0.52 0.58


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – –
Bud Neck – – – – – – – – – – – – – – –
Bud Site – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – –
Endosome – – – – – – – – – – – – – – –
Golgi – – – – – – – – – – – – – – –
Mitochondria – – – – – – – – – – – – – – –
Nuclear Periphery – – – – – – – – – – – – – – –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – –
Peroxisomes – – – – – – – – – – – – – – –
SpindlePole – – – – – – – – – – – – – – –
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – – –
Cytoplasm – – – – – – – – – – – – – – –
Nucleus – – – – – – – – – – – – – – –
Vacuole – – – – – – – – – – – – – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration -0.7461 -0.9216 -0.6884 -1.0851 -0.9571 -0.7339 -0.9151 -1.0043 -0.9867 -1.1822 -0.4938 -1.1906 -0.0828 0.2062 -0.0812 0.1821 0.3886 0.0995
Actin 0.0261 0.0001 0.0061 0.0074 0.0079 0.0098 0.0287 0.0003 0.0108 0.0092 0.0137 0.0035 0.0053 0.0001 0.0019 0.0414 0.0088 0.0015
Bud 0.002 0.0003 0.0015 0.0018 0.0069 0.0002 0.0017 0.0021 0.0018 0.0013 0.0016 0.0013 0.0011 0 0.0007 0.0042 0.0013 0.0001
Bud Neck 0.0022 0.0001 0.0031 0.0012 0.001 0.0004 0.0038 0.0012 0.0015 0.0002 0.0001 0.0008 0.0183 0.0001 0.0015 0.0006 0.0003 0.0048
Bud Periphery 0.0077 0.0003 0.0023 0.0042 0.0118 0.0006 0.0029 0.0027 0.0044 0.0181 0.0038 0.0012 0.0027 0.0001 0.0031 0.0114 0.0011 0.0003
Bud Site 0.0101 0.008 0.0112 0.0524 0.003 0.0002 0.0137 0.0106 0.0088 0.0018 0.0013 0.0008 0.0106 0.0058 0.0063 0.0134 0.009 0.0001
Cell Periphery 0.0007 0.0001 0.0005 0.0005 0.0005 0.0002 0.0004 0.0001 0.0002 0.0003 0.0001 0.0001 0.0015 0.0001 0.0002 0.0004 0.0001 0.0001
Cytoplasm 0.0384 0.0328 0.0303 0.0138 0.0287 0.0537 0.0297 0.0239 0.0369 0.0314 0.0456 0.0578 0.0261 0.027 0.0245 0.0654 0.0309 0.0661
Cytoplasmic Foci 0.0328 0.0077 0.0225 0.0282 0.0525 0.0255 0.0296 0.0035 0.0162 0.0116 0.0081 0.0093 0.0197 0.0009 0.0038 0.0119 0.037 0.0027
Eisosomes 0.0005 0.0001 0.0003 0.0005 0.0001 0.0002 0.0006 0.0002 0.0002 0.0002 0.0003 0.0001 0.0003 0.0002 0.0002 0.0002 0.0001 0.0001
Endoplasmic Reticulum 0.0054 0.0004 0.0022 0.001 0.0017 0.0004 0.0036 0.0002 0.0007 0.001 0.0014 0.0004 0.0008 0.0002 0.0003 0.0003 0.0004 0.0002
Endosome 0.0277 0.0005 0.0185 0.0017 0.0226 0.0055 0.0291 0.0001 0.011 0.0111 0.0297 0.003 0.0174 0.0001 0.0071 0.0009 0.0246 0.0003
Golgi 0.0066 0.0001 0.0118 0.0012 0.0134 0.0059 0.0101 0 0.0077 0.0048 0.0082 0.002 0.0064 0 0.0062 0.0006 0.0311 0.0003
Lipid Particles 0.0131 0.0007 0.0083 0.0046 0.0477 0.0051 0.0194 0.0002 0.0077 0.0066 0.0013 0.002 0.0046 0.0001 0.0008 0.001 0.0053 0.0001
Mitochondria 0.0131 0.0005 0.0177 0.0028 0.0464 0.0028 0.0173 0.0006 0.0064 0.0045 0.0135 0.0013 0.0015 0.0001 0.0015 0.0027 0.0069 0.0005
None 0.7647 0.9437 0.8476 0.8496 0.6685 0.8616 0.7728 0.9492 0.8627 0.8558 0.8316 0.8975 0.8675 0.9646 0.9399 0.828 0.8148 0.9193
Nuclear Periphery 0.0172 0.0002 0.0011 0.0018 0.0037 0.0036 0.0073 0.0004 0.0011 0.0039 0.0237 0.001 0.0009 0.0001 0.0001 0.0003 0.0003 0.0001
Nucleolus 0.0007 0.0004 0.0012 0.001 0.0009 0.0003 0.001 0.0006 0.001 0.0003 0.0004 0.0006 0.0009 0.0001 0.0001 0.007 0.0002 0.0001
Nucleus 0.0024 0.0007 0.0014 0.0011 0.0032 0.0006 0.0025 0.0008 0.0031 0.0009 0.0065 0.0027 0.001 0.0002 0.0002 0.0021 0.0003 0.0015
Peroxisomes 0.0194 0.0012 0.0054 0.0135 0.0576 0.0144 0.0171 0.0002 0.0139 0.0323 0.0027 0.0016 0.0039 0 0.0009 0.0049 0.0252 0.001
Punctate Nuclear 0.0029 0.0017 0.003 0.011 0.0059 0.0014 0.0047 0.0023 0.0021 0.0036 0.0011 0.0125 0.004 0.0002 0.0001 0.0012 0.0007 0.0005
Vacuole 0.0041 0.0003 0.0031 0.0006 0.0123 0.0062 0.0027 0.0004 0.0015 0.0006 0.0038 0.0005 0.005 0.0001 0.0005 0.0019 0.0011 0.0001
Vacuole Periphery 0.0022 0 0.0007 0.0003 0.0038 0.0015 0.0015 0.0002 0.0004 0.0006 0.0015 0.0001 0.0004 0 0.0001 0.0002 0.0004 0

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 1916.8632 1195.1711 188.7712 188.9763 1823.9434 1046.4465 756.486 524.0887 390.6922 1330.9675
Translational Efficiency 1.695 1.6421 1.851 1.7371 1.593 2.3914 1.7238 1.6635 1.5711 1.792

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✔
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1917 189 163 1331 1072 2088 3011 1404 2989 2277 3174 2735

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 657.36 751.05 911.05 785.21 538.86 718.52 798.08 794.83 614.86 721.22 803.88 790.15
Standard Deviation 76.70 107.17 85.14 107.55 58.86 88.85 90.52 105.32 90.81 90.96 93.63 106.52
Intensity Change Log 2 — 0.192225 0.470847 0.256395 — 0.415118 0.566623 0.560736 — 0.296911 0.514779 0.401478

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000076 0.000552 0.000266 0.000473 0.000189 0.000312 0.000149 0.000496 0.000117 0.000332 0.000155 0.000485
Bud Neck 0.020169 0.051968 0.001343 0.009034 0.028551 0.060185 0.010917 0.005976 0.023175 0.059503 0.010425 0.007464
Bud Site 0.003549 0.016384 0.001444 0.030024 0.006378 0.017795 0.021203 0.016195 0.004564 0.017678 0.020188 0.022925
Cell Periphery 0.000250 0.000152 0.000284 0.000209 0.000547 0.000222 0.000110 0.001137 0.000356 0.000216 0.000119 0.000685
Cytoplasm 0.474411* 0.288746* 0.232881* 0.450582* 0.388927* 0.336233* 0.435613* 0.470005* 0.443753* 0.332291* 0.425202* 0.460552*
Cytoplasmic Foci 0.221198* 0.279998* 0.054829 0.031461 0.308951* 0.254284* 0.004939 0.028028 0.252671* 0.256418* 0.007501 0.029699
Eisosomes 0.000283 0.000169 0.000151 0.000064 0.000800 0.000214 0.000017 0.000060 0.000468 0.000210 0.000024 0.000062
Endoplasmic Reticulum 0.000677 0.000896 0.003298 0.001957 0.000809 0.000907 0.000767 0.001059 0.000725 0.000906 0.000897 0.001496
Endosome 0.009921 0.036586 0.007844 0.007678 0.007196 0.037903 0.001179 0.003465 0.008944 0.037794 0.001521 0.005515
Golgi 0.001929 0.006709 0.000140 0.002532 0.006000 0.008789 0.000111 0.001392 0.003389 0.008617 0.000113 0.001947
Lipid Particles 0.013562 0.006704 0.006491 0.003343 0.032689 0.013792 0.000374 0.001385 0.020422 0.013204 0.000688 0.002338
Mitochondria 0.003605 0.010959 0.000291 0.003941 0.038787 0.025240 0.002643 0.001874 0.016223 0.024054 0.002522 0.002880
Mitotic Spindle 0.000151 0.017049 0.000161 0.030589 0.000075 0.007398 0.013874 0.015406 0.000124 0.008199 0.013170 0.022795
None 0.002230 0.001562 0.003484 0.005699 0.006725 0.001997 0.004468 0.007043 0.003842 0.001961 0.004418 0.006389
Nuclear Periphery 0.000403 0.000497 0.001565 0.001594 0.000835 0.000600 0.001405 0.001356 0.000558 0.000591 0.001413 0.001472
Nuclear Periphery Foci 0.000168 0.001098 0.001854 0.000997 0.000138 0.000223 0.000105 0.000445 0.000157 0.000296 0.000194 0.000713
Nucleolus 0.000875 0.002209 0.001892 0.000871 0.001848 0.002289 0.000277 0.000553 0.001224 0.002283 0.000360 0.000708
Nucleus 0.199216* 0.136178* 0.581152* 0.297069* 0.139161* 0.117483 0.476301* 0.353541* 0.177678* 0.119035 0.481686* 0.326059*
Peroxisomes 0.005249 0.045270 0.000770 0.001682 0.016328 0.019591 0.000781 0.000554 0.009222 0.021722 0.000781 0.001103
Vacuole 0.041414 0.091103 0.099547 0.115953 0.013258 0.091101 0.024161 0.088907 0.031316 0.091101 0.028033 0.102069
Vacuole Periphery 0.000661 0.005208 0.000314 0.004250 0.001806 0.003442 0.000605 0.001124 0.001071 0.003589 0.000590 0.002646

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -4.02 -5.79 -4.27 0.90 -0.45 -2.19 1.31 -1.30 -0.43 -1.65 -4.75 -2.06 -3.41 -0.73 -2.95
Bud Neck -3.51 13.90 7.73 5.03 -5.75 -8.82 7.44 9.55 19.44 4.32 -12.65 9.45 12.65 19.82 3.20
Bud Site -3.92 1.82 -6.30 -0.64 -7.11 -6.01 -4.96 -2.50 3.22 2.32 -8.55 -6.68 -6.90 1.34 0.11
Cell Periphery 1.15 -0.67 1.12 -0.26 5.82 4.89 8.78 -0.08 -0.81 -1.09 2.12 4.60 -0.09 -0.70 -1.18
Cytoplasm 8.89 15.11 11.09 -3.63 -8.24 5.44 0.19 0.09 -5.46 -0.09 15.09 9.59 9.30 -5.81 0.13
Cytoplasmic Foci -2.95 24.85 40.83 14.26 5.00 6.70 46.25 42.39 45.45 -12.92 -0.48 63.43 57.40 48.29 -15.20
Eisosomes 4.37 5.26 11.47 4.84 8.67 5.58 7.44 6.99 14.94 -13.83 6.40 11.11 10.06 15.83 -18.40
Endoplasmic Reticulum -1.28 -6.54 -11.72 -7.39 3.34 -0.52 -2.44 -7.73 -6.02 -7.60 -1.20 -5.80 -13.88 -9.44 -9.77
Endosome -4.76 1.05 5.58 5.71 3.83 -17.16 9.82 6.38 20.50 -4.84 -17.13 14.82 8.71 20.67 -6.56
Golgi -2.49 9.61 1.46 2.84 -1.98 -2.82 11.32 7.68 8.69 -1.34 -6.51 14.67 6.09 9.21 -2.44
Lipid Particles 4.19 6.13 11.13 2.76 5.44 8.07 14.90 14.41 14.05 -13.58 5.65 20.17 18.43 13.32 -5.48
Mitochondria -1.77 7.48 1.64 2.13 -2.52 3.74 10.98 11.31 14.64 2.06 -3.84 10.81 11.04 14.19 0.69
Mitotic Spindle -2.47 -0.91 -7.57 -0.58 -7.55 -5.77 -8.32 -6.13 -1.91 -0.17 -6.22 -8.28 -9.69 -3.96 -2.78
None 1.75 -1.47 -3.28 -4.05 -1.55 4.47 2.34 1.45 -4.05 -0.96 3.93 -0.83 -1.42 -5.11 -0.89
Nuclear Periphery -1.41 -11.38 -17.39 -12.81 -4.15 0.51 -2.69 -3.07 -14.84 -1.83 -0.30 -8.74 -10.83 -18.27 -4.62
Nuclear Periphery Foci -1.92 -8.89 -7.77 0.13 4.03 -2.06 -0.12 -8.63 -6.19 -9.57 -2.69 -4.21 -10.78 -6.42 -9.38
Nucleolus -2.17 -3.38 0.85 2.31 3.56 -0.79 4.25 3.66 4.67 -3.70 -2.83 6.07 3.97 4.66 -4.33
Nucleus 4.13 -15.61 -15.01 -11.69 9.48 3.50 -51.66 -26.46 -31.42 15.94 12.16 -55.74 -28.17 -36.74 22.91
Peroxisomes -4.31 8.58 7.48 4.81 -0.68 -1.93 13.05 13.29 16.76 0.56 -8.78 14.89 15.09 16.20 -0.51
Vacuole -5.00 -7.37 -24.16 -7.81 -4.32 -25.60 -13.94 -25.83 -11.30 -21.37 -19.94 -5.62 -33.85 -17.56 -30.39
Vacuole Periphery -1.88 6.10 -2.20 1.24 -2.74 -4.35 5.02 3.80 7.44 -0.64 -7.21 3.58 -0.64 4.92 -2.42
Standard name
Human Ortholog
Description Histone H3; core histone protein required for chromatin assembly, part of heterochromatin-mediated telomeric and HM silencing; one of two identical histone H3 proteins (see HHT2); regulated by acetylation, methylation, and phosphorylation; H3K14 acetylation plays an important role in the unfolding of strongly positioned nucleosomes during repair of UV damage
Localization
Cell Percentages —
Cell Cycle Regulation No
Subcompartmental Group N/A

Hht1

Hht1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Hht1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available