Standard name
Human Ortholog
Description Copper-binding protein of mitochondrial inner membrane; required for cytochrome c oxidase activity and respiration; may function to deliver copper to cytochrome c oxidase; similar to thioredoxins; SCO1 has a paralog, SCO2, that arose from the whole genome duplication
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches – 0 0 0 0 0 0 0 0 0 0 0 0.07 0 0 0.11 – – 0 0 0 0 0 0
Bud – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 – – 0 0 0 0 0 0
Bud Neck – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – 0 0 0 0 0 0
Bud Site – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 – – – – – – – –
Cell Periphery – 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0.05 – – 0 0 0 0 0 0
Cytoplasm – 0.05 0 0 0 0.05 0.05 0 0 0 0 0.05 0.07 0.11 0.11 0.05 – – 0 0 0 0 0 0
Endoplasmic Reticulum – 0 0 0 0 0 0 0 0 0 0 0 0.08 0.07 0 0.05 – – 0.09 0 0 0 0 0
Endosome – 0 0 0 0 0 0 0 0 0 0 0 0.08 0.12 0.31 0.05 – – 0.44 0.06 0.05 0.08 0.06 0
Golgi – 0 0 0 0 0 0 0 0 0 0 0 0.15 0.14 0.08 0.26 – – 0 0 0 0.05 0.05 0
Mitochondria – 0.82 0.93 0.94 0.92 0.95 0.91 0.94 0.95 0.94 0.98 0.97 0.5 0.39 0.18 0.16 – – 0 0.73 0.83 0.73 0.7 0.71
Nucleus – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.11 – – 0 0 0 0 0 0
Nuclear Periphery – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 – – 0.16 0 0 0 0 0
Nucleolus – 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0.05 – – 0 0 0 0 0 0
Peroxisomes – 0 0 0 0 0 0 0 0 0 0 0 0 0 0.09 0 – – 0 0 0 0.06 0.05 0
SpindlePole – 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0.07 0.05 – – 0 0 0 0 0 0
Vac/Vac Membrane – 0.19 0.07 0 0.1 0 0.08 0.06 0.05 0.07 0 0.05 0.18 0.22 0.3 0.11 – – 0 0.07 0 0 0.06 0.06
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches – 1 0 0 0 0 0 0 0 0 0 0 9 3 1 2 – – 0 6 2 3 5 7
Bud – 3 1 2 14 6 11 5 6 7 4 7 1 1 4 1 – – 0 2 2 1 0 1
Bud Neck – 0 0 0 0 1 0 0 0 0 0 0 1 0 0 0 – – 0 3 0 1 2 2
Bud Site – 0 0 0 0 3 5 5 4 7 2 0 0 0 0 1 – – – – – – – –
Cell Periphery – 13 2 4 0 2 1 1 0 1 3 2 3 7 6 1 – – 0 0 0 0 0 1
Cytoplasm – 18 7 7 14 11 14 10 7 10 4 12 9 13 19 1 – – 0 2 0 0 0 0
Endoplasmic Reticulum – 9 4 2 2 8 0 4 1 1 3 1 10 8 7 1 – – 0 1 1 0 1 3
Endosome – 16 4 2 12 1 1 5 0 1 0 0 10 14 52 1 – – 0 25 9 16 14 9
Golgi – 7 0 0 0 0 0 0 0 0 0 0 18 16 14 5 – – 0 16 2 9 12 7
Mitochondria – 291 163 172 304 204 250 279 190 306 174 218 61 46 30 3 – – 0 296 143 153 159 158
Nucleus – 2 1 0 4 2 6 4 3 4 1 1 1 1 1 2 – – 0 1 0 0 0 0
Nuclear Periphery – 2 1 0 1 9 4 5 4 13 8 7 0 0 0 1 – – 0 3 1 0 1 2
Nucleolus – 0 0 1 3 3 5 4 3 16 2 6 1 0 2 1 – – 0 1 0 0 0 0
Peroxisomes – 13 4 3 10 0 4 0 1 2 2 1 5 3 15 0 – – 0 10 6 11 12 9
SpindlePole – 8 2 2 4 2 6 8 3 15 3 4 4 2 11 1 – – 0 1 0 0 1 1
Vac/Vac Membrane – 68 12 5 34 8 22 17 10 22 3 11 22 26 50 2 – – 0 28 2 6 13 12
Unique Cell Count 357 175 183 329 214 274 297 200 325 178 225 121 118 166 19 2 405 174 211 229 223
Labelled Cell Count 451 201 200 402 260 329 347 232 405 209 270 155 140 212 23 2 405 174 211 229 223


Mitochondria

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) – 5.3 4.3 4.1 4.3 4.0 4.2 3.8 3.7 3.3 3.5 3.6 6.4 6.1 6.4 6.6 – – 6.7 6.9 7.0
Std Deviation (1e-4) – 0.8 0.9 1.4 1.2 0.9 3.2 1.0 1.1 0.7 1.1 0.9 1.0 0.7 1.0 1.2 – – 1.1 1.3 1.5
Intensity Change (Log2) – – – -0.06 -0.01 -0.09 -0.01 -0.18 -0.23 -0.36 -0.28 -0.26 0.58 0.52 0.59 0.62 – – 0.65 0.69 0.72


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 – –
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 – –
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 – –
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 – –
Cytoplasm -0.1 0.1 0.5 0.5 -0.4 -0.3 -0.5 -0.9 0.6 1.3 2.3 2.6 0 – –
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 2.4 0 1.0 0 – –
Endosome 0 0 0 0 0 0 0 0 0 2.4 3.3 7.2 0 – –
Golgi 0 0 0 0 0 0 0 0 0 5.3 5.0 3.9 0 – –
Mitochondria 0 0 0 0 0 0 0 0 0 -8.4 -10.1 -14.0 -9.1 – –
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 – –
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 – –
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 – –
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 2.7 0 – –
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 2.6 0 – –
Vacuole -1.8 1.3 -1.4 0.5 -0.5 -0.8 0 -2.4 -0.8 3.0 3.8 5.6 0 – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 1.735 1.3049 1.4897 1.1125 0.9699 1.374 3.361 3.3436 2.9503 2.7582 3.7697 2.9788 0.072 0.683 0.0044 0.0525 0.1286 0.2214
Actin 0.0222 0.0023 0.0099 0.0024 0.015 0.0035 0.0265 0.0298 0.0117 0.0159 0.1243 0.0201 0.0148 0.0128 0.0073 0.0017 0.0049 0.0049
Bud 0.0016 0.0033 0.0021 0.0019 0.0009 0.0009 0.0015 0.0033 0.0075 0.0017 0.0023 0.0016 0.0006 0.0033 0.0021 0.0009 0.0053 0.0006
Bud Neck 0.0013 0.0005 0.0054 0.0015 0.0042 0.0084 0.0025 0.0015 0.0052 0.0225 0.0154 0.0045 0.001 0.0006 0.0013 0.001 0.0009 0.0092
Bud Periphery 0.0025 0.0064 0.0047 0.0074 0.0014 0.0023 0.0047 0.0108 0.0172 0.006 0.0056 0.0025 0.0015 0.0046 0.0087 0.0046 0.0243 0.002
Bud Site 0.0053 0.0151 0.0165 0.0023 0.0061 0.0012 0.0177 0.051 0.0224 0.0024 0.0036 0.0021 0.0027 0.0157 0.0047 0.0016 0.0039 0.0009
Cell Periphery 0.0011 0.0006 0.0016 0.0005 0.0003 0.0006 0.0036 0.0022 0.0052 0.0009 0.0007 0.0008 0.0012 0.0006 0.0057 0.0017 0.0006 0.0007
Cytoplasm 0.0128 0.0095 0.0108 0.0056 0.0031 0.0102 0.0293 0.0042 0.0176 0.0124 0.0147 0.0082 0.0071 0.012 0.0059 0.0091 0.0042 0.0072
Cytoplasmic Foci 0.0479 0.0258 0.0604 0.0427 0.0505 0.0337 0.0458 0.0326 0.0334 0.0366 0.0584 0.0411 0.0316 0.0433 0.0304 0.0135 0.0406 0.029
Eisosomes 0.001 0.0001 0.0005 0.0002 0.0002 0.0002 0.0023 0.0017 0.002 0.0007 0.0012 0.0011 0.002 0.0003 0.001 0.0003 0.0002 0.0004
Endoplasmic Reticulum 0.0029 0.0015 0.0011 0.0009 0.0033 0.0052 0.0119 0.0021 0.0025 0.0009 0.0036 0.0028 0.0055 0.0036 0.0015 0.0009 0.0018 0.0013
Endosome 0.1103 0.0805 0.0922 0.1097 0.1066 0.0978 0.1172 0.1383 0.0825 0.0674 0.0951 0.0598 0.1143 0.111 0.0884 0.0501 0.1373 0.0778
Golgi 0.0501 0.0473 0.0604 0.0775 0.0707 0.0494 0.0466 0.0709 0.0676 0.0417 0.0987 0.068 0.0429 0.0662 0.0537 0.034 0.0724 0.0612
Lipid Particles 0.0372 0.0313 0.0174 0.0092 0.0093 0.0261 0.0343 0.0189 0.0119 0.0055 0.0164 0.0177 0.0485 0.01 0.0226 0.0019 0.0293 0.0179
Mitochondria 0.5857 0.7289 0.6162 0.6795 0.686 0.6914 0.4928 0.5899 0.6438 0.6808 0.5073 0.6897 0.6295 0.6302 0.7099 0.8536 0.5576 0.7318
None 0.0337 0.0032 0.0204 0.0213 0.0009 0.0133 0.0723 0.0155 0.0215 0.0312 0.0102 0.0163 0.0262 0.0194 0.0116 0.0056 0.006 0.0026
Nuclear Periphery 0.0096 0.0018 0.0006 0.0007 0.0074 0.0024 0.0224 0.0007 0.0006 0.0003 0.0017 0.0042 0.0035 0.0024 0.0016 0.0003 0.016 0.0009
Nucleolus 0.0019 0.0006 0.0033 0.0007 0.0003 0.0006 0.0021 0.0003 0.0017 0.0014 0.0009 0.0008 0.0016 0.0013 0.0007 0.0008 0.0041 0.0012
Nucleus 0.0038 0.0008 0.0017 0.0009 0.0009 0.001 0.0119 0.0004 0.0015 0.0014 0.0012 0.0022 0.0021 0.0012 0.0009 0.0019 0.0093 0.002
Peroxisomes 0.0474 0.0226 0.0535 0.0108 0.0237 0.0341 0.0233 0.0144 0.0226 0.0582 0.0246 0.0449 0.0319 0.037 0.0231 0.0077 0.0577 0.0333
Punctate Nuclear 0.0037 0.0002 0.0013 0.0004 0.0015 0.0008 0.008 0.0004 0.0007 0.0006 0.005 0.003 0.0044 0.0005 0.0004 0.0009 0.004 0.0012
Vacuole 0.0099 0.0114 0.0148 0.0137 0.0038 0.0089 0.0161 0.0074 0.014 0.0066 0.0052 0.0042 0.0171 0.0135 0.0107 0.0039 0.0103 0.0075
Vacuole Periphery 0.0079 0.006 0.0052 0.0105 0.0037 0.008 0.0076 0.0037 0.0068 0.0048 0.0036 0.0042 0.01 0.0105 0.0082 0.0039 0.0092 0.0062

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 13.6683 17.7725 16.485 16.9126 16.1105 24.8377 27.797 32.2161 35.4136 22.593
Translational Efficiency 1.7913 1.4743 1.2512 1.5112 1.3644 1.2314 1.3106 1.4618 1.1099 1.2939

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Copper-binding protein of mitochondrial inner membrane; required for cytochrome c oxidase activity and respiration; may function to deliver copper to cytochrome c oxidase; similar to thioredoxins; SCO1 has a paralog, SCO2, that arose from the whole genome duplication
Localization
Cell Percentages mitochondrion (95%)
Cell Cycle Regulation No
Subcompartmental Group mito-2

Sco1

Sco1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Sco1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available