Standard name | |
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Human Ortholog | |
Description | Putative glycoside hydrolase of the mitochondrial intermembrane space |
ensLOC | DeepLoc | |||||||||||||||||||||||
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Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 1 | 3 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 4 | 2 | 4 | 5 |
Bud | 1 | 0 | 4 | 1 | 2 | 2 | 3 | 8 | 5 | 3 | 10 | 12 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 8 | 11 | 19 |
Bud Neck | 0 | 0 | 1 | 0 | 4 | 1 | 1 | 6 | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 1 | 1 | 2 | 4 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 1 | 1 | 2 | 0 | 1 | 2 | 5 | 2 | 2 | 8 | 4 | 8 | 1 | 1 | 0 | 17 | 4 | 8 | 0 | 0 | 2 | 1 | 3 | 6 |
Cytoplasm | 173 | 227 | 179 | 157 | 251 | 225 | 356 | 374 | 244 | 316 | 203 | 280 | 356 | 622 | 691 | 277 | 204 | 139 | 150 | 205 | 160 | 292 | 405 | 341 |
Endoplasmic Reticulum | 1 | 1 | 2 | 0 | 1 | 2 | 3 | 0 | 3 | 4 | 6 | 1 | 2 | 1 | 0 | 18 | 10 | 4 | 0 | 1 | 0 | 2 | 1 | 2 |
Endosome | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 1 | 2 | 1 | 4 | 1 | 1 | 5 | 3 | 5 |
Golgi | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 4 | 0 | 0 | 0 | 4 | 0 |
Mitochondria | 24 | 22 | 9 | 16 | 12 | 53 | 112 | 100 | 95 | 152 | 145 | 155 | 4 | 2 | 1 | 12 | 5 | 2 | 12 | 16 | 18 | 20 | 33 | 29 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 2 | 1 | 2 | 4 | 2 | 2 | 2 | 1 | 3 | 1 | 5 | 0 | 1 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 4 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 1 | 0 |
Nucleolus | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 1 | 0 | 1 | 0 | 0 | 0 | 4 | 3 | 0 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 5 | 10 | 7 |
Vac/Vac Membrane | 0 | 0 | 0 | 0 | 0 | 0 | 8 | 20 | 10 | 11 | 5 | 10 | 1 | 1 | 1 | 9 | 2 | 4 | 2 | 2 | 3 | 3 | 6 | 17 |
Unique Cell Count | 186 | 239 | 188 | 161 | 258 | 246 | 418 | 437 | 300 | 418 | 298 | 366 | 361 | 628 | 694 | 286 | 210 | 149 | 188 | 238 | 197 | 352 | 500 | 453 |
Labelled Cell Count | 205 | 251 | 198 | 174 | 272 | 286 | 493 | 519 | 361 | 502 | 383 | 472 | 368 | 631 | 695 | 337 | 228 | 164 | 188 | 238 | 197 | 352 | 500 | 453 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 3.4 | 3.2 | 3.3 | 3.3 | 3.4 | 3.4 | 3.0 | 3.4 | 3.7 | 3.7 | 4.1 | 4.1 | 4.1 | 4.0 | 4.0 | 5.9 | 6.9 | 7.3 | 4.1 | 4.0 | 4.5 |
Std Deviation (1e-4) | 1.0 | 0.7 | 0.7 | 1.2 | 1.0 | 1.4 | 1.1 | 1.1 | 1.3 | 1.5 | 1.5 | 1.6 | 1.4 | 1.4 | 1.3 | 1.7 | 1.8 | 1.4 | 1.7 | 1.6 | 1.8 |
Intensity Change (Log2) | – | – | – | 0.02 | 0.07 | 0.04 | -0.13 | 0.05 | 0.17 | 0.18 | 0.34 | 0.32 | 0.33 | 0.29 | 0.3 | 0.86 | 1.07 | 1.17 | 0.32 | 0.28 | 0.46 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Actin | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud Neck | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud Site | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cell Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cyto | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endoplasmic Reticulum | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endosome | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Golgi | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Mitochondria | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nuclear Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nuc | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleolus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Peroxisomes | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
SpindlePole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Vac | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cortical Patches | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cytoplasm | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Vacuole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 2.7331 | 3.7528 | 3.4143 | 3.2264 | 2.8546 | 3.2938 | -0.2545 | -0.1812 | -0.3949 | -0.2031 | -0.109 | -0.3506 | 0.463 | 0.1754 | 0.3683 | 0.6139 | 0.2912 | 0.2883 |
Actin | 0.0007 | 0.0022 | 0.001 | 0.0008 | 0.0009 | 0.0009 | 0.0805 | 0.0003 | 0.0116 | 0.0505 | 0.0476 | 0.0137 | 0.0597 | 0.0052 | 0.0097 | 0.0368 | 0.111 | 0.0003 |
Bud | 0.0004 | 0.0054 | 0.0013 | 0.0004 | 0.0001 | 0.0001 | 0.0028 | 0.0002 | 0.0005 | 0.001 | 0.0006 | 0.0008 | 0.0013 | 0.0005 | 0.0025 | 0.0007 | 0.0014 | 0.0064 |
Bud Neck | 0.0011 | 0.0005 | 0.0051 | 0.0004 | 0.0005 | 0.0023 | 0.0174 | 0.0003 | 0.0013 | 0.0005 | 0.0006 | 0.0008 | 0.0011 | 0.0015 | 0.0001 | 0.0016 | 0.0007 | 0.0002 |
Bud Periphery | 0.0007 | 0.0049 | 0.0013 | 0.0004 | 0.0002 | 0.0003 | 0.0048 | 0.0001 | 0.0014 | 0.0025 | 0.0008 | 0.0023 | 0.0029 | 0.0008 | 0.0024 | 0.0008 | 0.0046 | 0.0041 |
Bud Site | 0.008 | 0.0161 | 0.0049 | 0.0009 | 0.0001 | 0.0005 | 0.0184 | 0.0222 | 0.0008 | 0.002 | 0.0034 | 0.0004 | 0.0125 | 0.0017 | 0.0021 | 0.0074 | 0.0009 | 0.0002 |
Cell Periphery | 0.0005 | 0.0007 | 0.0004 | 0.0001 | 0.0001 | 0.0001 | 0.0008 | 0.0002 | 0.0002 | 0.0003 | 0.0003 | 0.0001 | 0.0009 | 0.0005 | 0.0002 | 0.0002 | 0.0001 | 0.0001 |
Cytoplasm | 0.2036 | 0.4062 | 0.3283 | 0.3575 | 0.3792 | 0.34 | 0.047 | 0.0854 | 0.1258 | 0.0809 | 0.0691 | 0.1031 | 0.1526 | 0.2587 | 0.1581 | 0.2241 | 0.1347 | 0.2371 |
Cytoplasmic Foci | 0.0053 | 0.0066 | 0.0067 | 0.0091 | 0.0067 | 0.0083 | 0.0801 | 0.0159 | 0.0055 | 0.0061 | 0.0297 | 0.011 | 0.0548 | 0.0136 | 0.0052 | 0.0287 | 0.0216 | 0.018 |
Eisosomes | 0.0002 | 0.0002 | 0.0002 | 0 | 0.0001 | 0.0001 | 0.0005 | 0.0003 | 0.0004 | 0.0004 | 0.0008 | 0.0002 | 0.0006 | 0.0002 | 0.0002 | 0.0003 | 0.0004 | 0 |
Endoplasmic Reticulum | 0.0028 | 0.0022 | 0.0018 | 0.0013 | 0.002 | 0.0013 | 0.0009 | 0.0012 | 0.0018 | 0.0017 | 0.0019 | 0.0004 | 0.008 | 0.0023 | 0.0025 | 0.0008 | 0.0018 | 0.0009 |
Endosome | 0.0016 | 0.0019 | 0.0016 | 0.0088 | 0.0012 | 0.0017 | 0.0075 | 0.0009 | 0.0016 | 0.0069 | 0.017 | 0.006 | 0.0206 | 0.0199 | 0.0034 | 0.0059 | 0.0518 | 0.0088 |
Golgi | 0.0002 | 0.0002 | 0.0002 | 0.0006 | 0.0002 | 0.0002 | 0.0054 | 0.0002 | 0.0008 | 0.0142 | 0.011 | 0.0051 | 0.0196 | 0.0015 | 0.0006 | 0.0106 | 0.0162 | 0.0004 |
Lipid Particles | 0.0005 | 0.0003 | 0.0002 | 0.0011 | 0.0003 | 0.0003 | 0.0102 | 0.0009 | 0.0013 | 0.0018 | 0.0163 | 0.0056 | 0.0557 | 0.0122 | 0.0005 | 0.0062 | 0.0121 | 0.001 |
Mitochondria | 0.0003 | 0.0004 | 0.0004 | 0.0004 | 0.0002 | 0.0002 | 0.0018 | 0.0005 | 0.0008 | 0.0042 | 0.0038 | 0.0064 | 0.0088 | 0.0017 | 0.0009 | 0.0085 | 0.0042 | 0.0008 |
None | 0.7679 | 0.5462 | 0.6424 | 0.6112 | 0.6043 | 0.6407 | 0.6555 | 0.8679 | 0.8394 | 0.8238 | 0.733 | 0.8374 | 0.5511 | 0.6433 | 0.7874 | 0.6148 | 0.5753 | 0.716 |
Nuclear Periphery | 0.0006 | 0.0006 | 0.0004 | 0.0009 | 0.0005 | 0.0004 | 0.0003 | 0.0005 | 0.0012 | 0.0006 | 0.0013 | 0.0005 | 0.0021 | 0.0006 | 0.0051 | 0.0005 | 0.0018 | 0.0003 |
Nucleolus | 0.0004 | 0.0003 | 0.0003 | 0.0003 | 0.0002 | 0.0001 | 0.0009 | 0.0002 | 0.0004 | 0.0001 | 0.0021 | 0.0001 | 0.0039 | 0.0003 | 0.0004 | 0.0002 | 0.0001 | 0.0002 |
Nucleus | 0.0016 | 0.0021 | 0.0013 | 0.0014 | 0.0008 | 0.0007 | 0.0007 | 0.0003 | 0.0005 | 0.0003 | 0.0015 | 0.0002 | 0.001 | 0.0008 | 0.0047 | 0.0003 | 0.0004 | 0.001 |
Peroxisomes | 0.0002 | 0.0002 | 0.0002 | 0.0004 | 0.0007 | 0.0002 | 0.0507 | 0.0008 | 0.0017 | 0.0008 | 0.0506 | 0.0044 | 0.0268 | 0.0249 | 0.0099 | 0.0365 | 0.0477 | 0.0006 |
Punctate Nuclear | 0.0015 | 0.0011 | 0.0006 | 0.0023 | 0.0013 | 0.0009 | 0.0027 | 0.0008 | 0.0018 | 0.0005 | 0.0063 | 0.0013 | 0.005 | 0.0007 | 0.0023 | 0.0144 | 0.0015 | 0.0025 |
Vacuole | 0.0019 | 0.0014 | 0.0013 | 0.0013 | 0.0004 | 0.0005 | 0.0107 | 0.0007 | 0.0009 | 0.0007 | 0.0019 | 0.0002 | 0.0093 | 0.0087 | 0.0013 | 0.0006 | 0.0097 | 0.0009 |
Vacuole Periphery | 0.0001 | 0.0001 | 0.0001 | 0.0002 | 0.0001 | 0.0001 | 0.0004 | 0.0001 | 0.0002 | 0.0002 | 0.0004 | 0.0001 | 0.0018 | 0.0003 | 0.0003 | 0.0002 | 0.002 | 0.0002 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 41.396 | 11.1344 | 18.4684 | 39.692 | 25.6484 | 26.3616 | 29.4694 | 34.1927 | 46.7789 | 36.5375 |
Translational Efficiency | 1.23 | 2.2118 | 1.5422 | 1.2194 | 1.404 | 2.1272 | 1.1436 | 1.093 | 1.1905 | 1.2438 |
Dataset | Hit |
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Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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