Standard name
Human Ortholog
Description AAA ATPase and SNARE disassembly chaperone; required for vesicular transport between ER and Golgi, the 'priming' step in homotypic vacuole fusion, autophagy, and protein secretion; releases Sec17p from SNAP complexes; has similarity to mammalian N-ethylmaleimide-sensitive factor (NSF)
DOWNLOAD

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0.22 0.23 0.21 0.22 0.17 0.15 0.16 0.16 – 0.17 0.21 0.18 0.22 0.23 0.14 0.2 0.15 0.17 0.11 0.11 0.21 0.18 0.18 0.12
Bud 0 0 0 0 0.05 0.07 0.07 0.06 – 0.06 0.1 0.07 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0.29 0.07 0.11 0.14 0.11 0.09 0.09 – 0.06 0.11 0.1 0.26 0.23 0.28 0.21 0.2 0.26 0 0.07 0 0 0 0
Endoplasmic Reticulum 0.3 0.4 0.25 0.3 0.27 0.28 0.23 0.19 – 0.19 0.13 0.25 0.51 0.37 0.23 0 0 0 0.09 0.15 0.08 0.12 0.16 0.13
Endosome 0 0 0.11 0.05 0 0.06 0 0 – 0.05 0 0 0 0 0 0.2 0.27 0.07 0.15 0.19 0.14 0.15 0.1 0.12
Golgi 0.6 0.19 0.53 0.61 0.56 0.3 0.38 0.32 – 0.2 0.15 0.23 0.4 0.44 0.48 0.28 0.23 0.53 0.58 0.31 0.49 0.42 0.46 0.55
Mitochondria 0.07 0.16 0.17 0.3 0.18 0.48 0.45 0.5 – 0.63 0.6 0.57 0 0 0 0.16 0.12 0.07 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0.07 0.06 0 0 0 0 0 0
Vac/Vac Membrane 0.16 0 0.06 0 0.05 0 0 0 – 0 0 0 0 0.05 0.07 0.16 0.17 0.2 0 0.09 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 57 46 68 43 46 47 64 82 – 80 49 50 25 53 25 37 21 15 32 29 79 44 55 47
Bud 10 6 11 3 13 23 27 29 – 27 23 19 4 0 2 4 5 1 1 3 2 2 3 3
Bud Neck 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 1 0 1 2 0
Bud Site 0 0 0 0 0 0 0 1 – 1 1 1 0 0 0 0 0 0 – – – – – –
Cell Periphery 1 3 1 1 2 1 5 3 – 1 1 4 0 3 4 0 0 0 0 0 0 0 0 0
Cytoplasm 9 58 24 22 38 34 37 44 – 29 26 27 29 52 49 38 28 23 4 17 0 5 5 5
Endoplasmic Reticulum 79 80 80 58 73 90 92 95 – 87 29 69 57 83 40 4 4 3 27 38 30 29 52 49
Endosome 10 3 35 9 10 18 17 20 – 22 10 3 3 2 4 37 38 6 46 48 53 36 33 45
Golgi 159 38 174 117 150 98 155 162 – 93 35 61 45 100 84 52 32 47 174 80 185 101 144 214
Mitochondria 19 32 57 58 47 154 183 257 – 288 139 154 1 2 0 30 17 6 4 4 4 8 7 8
Nucleus 0 1 0 0 2 0 0 1 – 1 1 0 0 0 1 5 9 1 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 2 0 2 2 – 2 0 3 1 0 0 0 1 0 0 4 0 0 0 0
Nucleolus 2 2 3 1 3 5 8 22 – 11 8 2 0 0 0 4 5 1 0 0 0 0 0 1
Peroxisomes 0 0 4 1 1 0 2 0 – 0 0 1 0 0 0 0 0 0 1 0 4 4 1 2
SpindlePole 0 0 4 2 0 0 0 0 – 0 1 0 0 0 0 6 10 5 0 1 2 0 2 1
Vac/Vac Membrane 42 7 20 0 14 13 17 16 – 14 4 2 4 11 13 30 23 18 3 22 6 7 7 9
Unique Cell Count 263 198 326 193 267 322 408 513 458 230 271 112 226 175 185 139 89 299 257 375 244 318 392
Labelled Cell Count 388 276 481 315 401 483 609 734 656 327 396 169 306 222 247 193 126 299 257 375 244 318 392


Early Golgi, Golgi

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 17.4 14.6 14.3 13.9 15.8 13.9 14.5 13.7 – 13.4 13.0 14.4 15.2 16.2 17.1 11.1 11.0 16.2 15.9 16.6 18.0
Std Deviation (1e-4) 4.2 3.7 3.2 3.1 4.0 3.7 4.2 3.5 – 4.6 4.6 5.2 3.5 3.6 4.5 3.5 3.8 5.4 2.8 3.6 4.2
Intensity Change (Log2) – – – -0.03 0.14 -0.04 0.02 -0.06 – -0.09 -0.14 0.01 0.09 0.19 0.26 -0.36 -0.37 0.18 0.15 0.22 0.34

WT3RAP60RAP140RAP220RAP300RAP380RAP540RAP620RAP70005101520WT3HU80HU120HU16005101520WT3rpd3Δ_1rpd3Δ_2rpd3Δ_305101520WT1AF100AF140AF18005101520
rapamycinhydroxy-urearpd3 knockoutalpha-factorMultiple Condition Time-Course Intensity PlotsGFP Intensity (1e-4)GFP Intensity (1e-4)GFP Intensity (1e-4)GFP Intensity (1e-4)

Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0.4 -1.1 -2.1 -1.8 -1.8 – -1.2 0.1 -0.7 0.3 0.7 -1.8 -0.2 -1.4 -0.8
Bud -1.2 0.9 2.2 2.0 1.5 – 1.6 3.2 2.0 0 0 0 -0.8 0 0
Bud Neck 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Cytoplasm 1.6 2.7 1.4 0.8 0.6 – -0.6 1.6 1.1 5.2 5.2 6.2 4.4 4.0 4.9
Endoplasmic Reticulum 1.4 0.8 1.0 -0.6 -2.1 – -1.9 -3.5 0.3 5.2 3.1 -0.4 -6.6 -5.6 -4.4
Endosome -2.4 -3.2 -2.4 -3.4 -3.9 – -3.2 -2.7 -4.8 -2.6 -4.6 -3.4 2.9 4.5 -1.1
Golgi 1.6 0.7 -5.9 -4.2 -6.3 – -9.6 -9.1 -7.7 -2.4 -2.1 -1.1 -5.5 -6.0 -0.1
Mitochondria 3.3 0 8.2 7.9 9.5 – 12.6 10.4 10.0 -4.5 -6.2 -5.9 -0.4 -1.4 -2.5
Nucleus 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 2.8 – 1.5 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Vacuole -3.5 -0.5 -1.2 -1.2 -2.1 – -2.1 -2.5 -3.5 -1.0 -0.6 0.6 3.7 3.5 4.1

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration – – – – – – – – – – – – – – – – – –
Actin 0.1557 0.0989 0.0602 0.0613 0.0717 0.084 0.1405 0.0366 0.0452 0.0664 0.0411 0.0397 – – – – – –
Bud 0.0055 0.0024 0.0171 0.0052 0.0003 0.0017 0.0005 0.0005 0.0146 0.0007 0.0012 0.0002 – – – – – –
Bud Neck 0.0041 0.0015 0.0017 0.0023 0.0014 0.0439 0.0013 0.0004 0.0012 0.0008 0.0009 0.0137 – – – – – –
Bud Periphery 0.0085 0.0017 0.0195 0.0061 0.0002 0.0009 0.0007 0.0002 0.0131 0.0004 0.0019 0.0002 – – – – – –
Bud Site 0.0115 0.0187 0.0119 0.0019 0.0003 0.0005 0.0024 0.0103 0.0075 0.0063 0.0006 0.0002 – – – – – –
Cell Periphery 0.0062 0.0008 0.0012 0.001 0.0002 0.0005 0.0006 0.0002 0.0005 0.0002 0.0008 0.0015 – – – – – –
Cytoplasm 0.1053 0.0477 0.0583 0.0254 0.017 0.0807 0.0101 0.0262 0.0196 0.0763 0.0308 0.0144 – – – – – –
Cytoplasmic Foci 0.0338 0.0619 0.0653 0.0767 0.1276 0.0493 0.0375 0.0424 0.0315 0.0311 0.0328 0.029 – – – – – –
Eisosomes 0.0013 0.0002 0.0001 0.0002 0.0001 0.0002 0.002 0 0.0002 0.0002 0.0001 0.0002 – – – – – –
Endoplasmic Reticulum 0.0317 0.003 0.0107 0.0043 0.0023 0.0096 0.0231 0.005 0.0057 0.0033 0.0117 0.0037 – – – – – –
Endosome 0.1648 0.2452 0.27 0.238 0.1862 0.1331 0.1359 0.2001 0.1861 0.2188 0.1859 0.106 – – – – – –
Golgi 0.356 0.4735 0.4051 0.459 0.5596 0.5528 0.5937 0.6495 0.6446 0.5583 0.6012 0.7697 – – – – – –
Lipid Particles 0.0255 0.0213 0.0178 0.0653 0.0246 0.0073 0.015 0.015 0.0061 0.0153 0.0219 0.0084 – – – – – –
Mitochondria 0.0079 0.0128 0.0159 0.0058 0.0029 0.0146 0.0111 0.0067 0.0033 0.003 0.0038 0.0081 – – – – – –
None 0.0058 0.0001 0.0011 0.0002 0.0001 0.0009 0.001 0.0001 0.0004 0.0012 0.0012 0.0001 – – – – – –
Nuclear Periphery 0.0339 0.0005 0.0032 0.0038 0.0002 0.0063 0.0128 0.0007 0.0022 0.0019 0.0164 0.0002 – – – – – –
Nucleolus 0.0006 0.0003 0.0002 0.0002 0 0.0007 0.0001 0 0.0001 0.0001 0.0006 0 – – – – – –
Nucleus 0.0101 0.0002 0.0007 0.0004 0.0001 0.0023 0.001 0.0001 0.0003 0.0008 0.0016 0.0001 – – – – – –
Peroxisomes 0.005 0.0013 0.0056 0.002 0.0018 0.0011 0.0049 0.0011 0.0016 0.0046 0.0314 0.0006 – – – – – –
Punctate Nuclear 0.0181 0.0002 0.0011 0.0008 0.0004 0.0008 0.0016 0.0001 0.0008 0.0015 0.0017 0.0001 – – – – – –
Vacuole 0.0039 0.0041 0.0174 0.0143 0.0013 0.0039 0.0022 0.0027 0.0062 0.0038 0.0046 0.0016 – – – – – –
Vacuole Periphery 0.0048 0.0037 0.0156 0.0259 0.0018 0.0048 0.0022 0.0022 0.0093 0.0048 0.0078 0.0023 – – – – – –

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 59.8465 43.3191 44.0382 54.4851 48.6923 51.8221 57.0494 53.8028 62.1102 61.0584
Translational Efficiency 1.7945 1.9787 1.5592 1.3062 1.5435 2.4248 1.6694 1.4271 1.3082 1.4575

Hit Data

Dataset Hit
Protein Concentration –
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✔

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
983 1513 1748 325 403 1823 1517 1983 1386 3336 3265 2308

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 1559.99 1243.80 1475.64 1634.47 1390.57 1378.46 1401.22 1261.33 1510.73 1317.39 1441.06 1313.87
Standard Deviation 334.85 296.19 233.72 295.43 252.39 273.92 256.33 153.62 322.44 292.04 247.29 222.29
Intensity Change Log 2 — -0.326782 -0.080196 0.067286 — -0.012619 0.011007 -0.140731 — -0.170178 -0.036494 -0.027027

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.025973 0.051845 0.013051 0.014532 0.066314 0.033437 0.048670 0.037337 0.037702 0.041786 0.029601 0.034126
Bud Neck 0.004236 0.002882 0.005688 0.006829 0.009898 0.001899 0.005457 0.005072 0.005882 0.002345 0.005580 0.005319
Bud Site 0.034395 0.019522 0.042893 0.042731 0.077689 0.012482 0.041922 0.065101 0.046983 0.015675 0.042442 0.061951
Cell Periphery 0.003972 0.002072 0.004196 0.004785 0.031328 0.002122 0.004504 0.007824 0.011926 0.002100 0.004339 0.007397
Cytoplasm 0.004447 0.005716 0.014776 0.005042 0.020127 0.002018 0.032148 0.036539 0.009006 0.003695 0.022847 0.032104
Cytoplasmic Foci 0.047427 0.097086 0.140036 0.077780 0.085905 0.072206 0.126370 0.179569 0.058615 0.083490 0.133686 0.165236
Eisosomes 0.000497 0.000775 0.000241 0.000279 0.002189 0.000916 0.002260 0.001844 0.000989 0.000852 0.001179 0.001623
Endoplasmic Reticulum 0.035006 0.031342 0.056820 0.084629 0.121371 0.037314 0.045644 0.042460 0.060118 0.034606 0.051628 0.048398
Endosome 0.196302 0.208243 0.227336 0.212205 0.199179 0.249324 0.182911 0.230010 0.197138 0.230693 0.206695 0.227503
Golgi 0.436355 0.415338 0.259259 0.275484 0.297184 0.441061 0.236117 0.207415 0.395889 0.429395 0.248507 0.217000
Lipid Particles 0.088241 0.140185 0.114038 0.080803 0.042656 0.113835 0.228358 0.105980 0.074986 0.125786 0.167154 0.102435
Mitochondria 0.005100 0.005011 0.000972 0.000885 0.004172 0.007231 0.007593 0.006151 0.004830 0.006224 0.004048 0.005410
Mitotic Spindle 0.002233 0.000565 0.005038 0.001682 0.005379 0.001499 0.000524 0.000264 0.003148 0.001075 0.002941 0.000464
None 0.000092 0.000349 0.000048 0.000046 0.000054 0.000073 0.000131 0.000044 0.000081 0.000198 0.000086 0.000044
Nuclear Periphery 0.000498 0.000127 0.000406 0.001222 0.000123 0.000180 0.000074 0.000041 0.000389 0.000156 0.000252 0.000207
Nuclear Periphery Foci 0.003905 0.001333 0.007961 0.013352 0.001577 0.000727 0.001252 0.000675 0.003228 0.001002 0.004844 0.002460
Nucleolus 0.000025 0.000115 0.000019 0.000018 0.000014 0.000026 0.000024 0.000028 0.000022 0.000066 0.000021 0.000026
Nucleus 0.000079 0.000107 0.000143 0.000101 0.000208 0.000094 0.000260 0.000184 0.000117 0.000100 0.000197 0.000172
Peroxisomes 0.001599 0.002019 0.001094 0.000737 0.004954 0.001266 0.015527 0.016625 0.002575 0.001607 0.007800 0.014387
Vacuole 0.081293 0.008600 0.089157 0.155086 0.017600 0.010359 0.013252 0.038147 0.062774 0.009561 0.053889 0.054613
Vacuole Periphery 0.028325 0.006767 0.016827 0.021772 0.012082 0.011929 0.007004 0.018691 0.023602 0.009588 0.012263 0.019125

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -5.64 3.91 2.96 8.77 -0.50 4.62 2.35 4.04 -1.18 2.84 -1.14 2.39 1.01 2.65 -1.64
Bud Neck 2.54 -2.38 -2.33 -3.76 -0.98 6.88 3.49 4.08 -8.24 0.61 7.17 0.53 1.13 -8.15 0.69
Bud Site 3.81 -2.00 -1.18 -3.79 0.06 8.87 4.62 1.60 -16.56 -5.93 9.06 1.21 -3.46 -16.14 -6.06
Cell Periphery 2.95 -0.34 -0.53 -1.91 -0.39 6.85 6.29 5.48 -9.24 -5.37 7.24 5.55 3.17 -9.63 -5.36
Cytoplasm -0.76 -7.20 -0.35 0.32 6.23 6.26 -3.52 -4.92 -20.26 -1.62 4.87 -9.06 -12.57 -18.55 -4.93
Cytoplasmic Foci -11.75 -21.34 -4.88 3.06 9.49 2.31 -6.06 -14.21 -26.99 -10.76 -7.94 -21.16 -26.42 -22.27 -7.98
Eisosomes -3.49 4.26 3.22 7.61 -0.96 3.57 -0.17 0.95 -5.81 1.99 1.10 -1.39 -4.10 -6.22 -3.31
Endoplasmic Reticulum 1.11 -5.49 -5.00 -5.45 -2.78 8.68 7.74 8.12 -1.61 0.96 6.73 2.09 2.74 -4.86 1.03
Endosome -1.84 -4.82 -1.64 -0.39 1.66 -5.30 1.73 -3.32 3.23 -7.93 -6.32 -1.81 -5.39 0.67 -4.49
Golgi 1.76 17.15 10.39 9.80 -1.20 -10.09 4.37 6.65 32.03 4.18 -3.92 17.45 20.98 35.36 5.65
Lipid Particles -6.75 -3.82 0.76 5.91 3.57 -10.64 -22.45 -10.04 1.33 15.98 -9.43 -16.25 -5.07 4.69 12.24
Mitochondria 0.06 3.80 3.80 3.76 0.27 -1.85 -2.36 -1.47 0.85 1.46 -1.22 0.85 -0.59 0.87 -2.14
Mitotic Spindle 3.06 -3.35 0.87 -3.21 4.49 1.36 1.73 1.82 1.76 3.91 2.15 0.11 2.97 1.56 6.62
None -2.01 2.15 2.25 2.43 0.32 -1.49 -7.01 1.21 2.64 9.90 -1.95 -0.22 2.55 2.71 7.83
Nuclear Periphery 8.87 1.24 -1.66 -2.51 -1.84 -2.15 3.17 5.60 6.13 7.21 7.67 3.25 2.71 -0.81 0.70
Nuclear Periphery Foci 5.62 -5.08 -3.48 -4.48 -1.92 2.91 1.06 3.19 0.45 4.84 6.90 -3.38 1.56 -3.62 4.46
Nucleolus -1.27 0.71 0.94 1.38 0.52 -1.02 -2.63 -3.80 -0.11 -1.86 -1.35 0.09 -0.75 1.24 -2.34
Nucleus -1.26 -4.10 -1.56 0.25 2.92 2.04 -1.13 0.56 -2.50 3.94 0.71 -4.55 -3.42 -3.30 2.37
Peroxisomes -0.84 1.35 2.23 3.58 2.72 5.60 -6.66 -9.13 -13.62 -0.62 2.57 -6.86 -11.64 -13.04 -5.59
Vacuole 18.02 -1.56 -6.26 -13.23 -5.71 3.76 2.12 -8.21 -14.83 -12.72 17.66 2.50 2.20 -19.01 -0.16
Vacuole Periphery 11.05 5.50 2.26 -6.75 -2.13 0.07 2.62 -3.17 -5.62 -12.35 9.05 7.31 2.66 -9.97 -7.14
Protein Complexes26S Proteasome complex37S mitochondrial small ribosomal subunit54S mitochondrial large ribosomal subunit60S cytosolic large ribosomal subunitAar2-U5 snRNPAnaphase-Promoting Complex variant 1Anaphase-Promoting Complex variant 2Anaphase-Promoting Complex variant 3Anaphase-Promoting Complex variant 4Arp2/3 complexATG1 kinase complexBLOC-1 complexCcr4-Not complexCentral kinetochore CTF19 complexCleavage and polyadenylation specificity factorcomplexCMG helicase complexCMG-Pol epsilon complexCOMPASS complexCOP9 signalosome COPI vesicle coat complexCoQ6 biosynthesis complexCore mediator complexCORVET complexCTF18-RFC complexCURI complex variant 1CURI complex variant 2CURI complex variant 3Cytoplasmic exosome complex, DIS3 variantDASH complexDNA-directed RNA Polymerase I complexDNA-directed RNA polymerase II complexDNA-directed RNA polymerase III complexDNA replication factor C complexEGO complexELG1-RFC complex Elongator holoenzyme complexEndoplasmic Reticulum Membrane ComplexEukaryotic translation initiation factor 2BcomplexEukaryotic translation initiation factor 3core complexExocystExomer complexFAR complexGeneral transcription factor complex TFIIDGeneral transcription factor complex TFIIHGET4-GET5 transmembrane domain recognition complexGID ubiquitin ligase complexGin4 complexGolgi transport complexGSE complexHMC complexHOPS complexINO80 chromatin remodeling complexLSM1-7 complexLsm1-7-Pat1 complexLSM2-7 complexLSM2-8 complexMCM complexMICOS complexMitochondrial electron transport complex IIIMitochondrial inner membrane pre-sequence translocasecomplexMitochondrial outer membrane translocase holocomplexMitochondrial proton-transporting ATP synthase complexMitochondrial pyruvate dehydrogenase complexNineTeen complexNOP8 60s ribosome pre-assembly complexNuA3 histone acetyltransferase complexNuA4 histone acetyltransferase complexNuclear condensin complexNuclear/nucleolar exosome complex, DIS3-RRP6 variantNuclear pore complexNucleolar ribonuclease MRP complexNucleolar ribonuclease P complexOligosaccharyl transferase complex variant 1Oligosaccharyl transferase complex variant 2PAF1 complexPAS complexPhosphatidylinositol 3-kinase complex IPolarisomePrefoldin complexPRP19-associated complexRad17 RFC-like complexRetromer complexRPD3L histone deacetylase complexRPD3S histone deacetylase complexRQC complexRSC complex variant RSC1RSC complex variant RSC2SAGA complexSCF-Met30 ubiquitin ligase complexSCF-Ufo1 ubiquitin ligase complexSEA complexSeptin complexSET3C histone deacetylase complexSignal recognition particleSLIK (SAGA-like) complexSmall ribosomal subunit processome, variant1Small ribosomal subunit processome, variant2Small ribosomal subunit processome, variant3SMC5-SMC6 SUMO ligase complexSm complexSpliceosomal commitment complexSPOTS complexSWI/SNF chromatin remodelling complexSwr1 chromatin remodelling complexTHO complexTranscription factor TFIIIC complexTranslocon complexTRAPPIII protein complexTRAPPII protein complexTRAPPI protein complexTREX complexU1 snRNPU2 snRNPU4 snRNPU4/U6 snRNPU4/U6.U5 tri-snRNP complexU5 snRNPU6 snRNP complexUTP-A complexUTP-B complexUTP-C complex variant 1UTP-C complex variant 2UTP-C complex variant 3Vacuolar proton translocating ATPase complex,Golgi variantVacuolar proton translocating ATPase complex,vacuole variantCellular Components••••••••••••••••actinbud neckcell peripherycytoplasmendosomeERGolgimitochondrionmixednuclear peripherynucleolusnucleusperoxisomespindle polevacuolar membranevacuole
Standard name
Human Ortholog
Description AAA ATPase and SNARE disassembly chaperone; required for vesicular transport between ER and Golgi, the 'priming' step in homotypic vacuole fusion, autophagy, and protein secretion; releases Sec17p from SNAP complexes; has similarity to mammalian N-ethylmaleimide-sensitive factor (NSF)
Localization
Cell Percentages vacuole (3%), Golgi (2%), mixed (52%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Sec18

Sec18


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Sec18-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available