Standard name | |
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Human Ortholog | |
Description | AAA ATPase and SNARE disassembly chaperone; required for vesicular transport between ER and Golgi, the 'priming' step in homotypic vacuole fusion, autophagy, and protein secretion; releases Sec17p from SNAP complexes; has similarity to mammalian N-ethylmaleimide-sensitive factor (NSF) |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 57 | 46 | 68 | 43 | 46 | 47 | 64 | 82 | – | 80 | 49 | 50 | 25 | 53 | 25 | 37 | 21 | 15 | 32 | 29 | 79 | 44 | 55 | 47 |
Bud | 10 | 6 | 11 | 3 | 13 | 23 | 27 | 29 | – | 27 | 23 | 19 | 4 | 0 | 2 | 4 | 5 | 1 | 1 | 3 | 2 | 2 | 3 | 3 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 2 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | – | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 1 | 3 | 1 | 1 | 2 | 1 | 5 | 3 | – | 1 | 1 | 4 | 0 | 3 | 4 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 9 | 58 | 24 | 22 | 38 | 34 | 37 | 44 | – | 29 | 26 | 27 | 29 | 52 | 49 | 38 | 28 | 23 | 4 | 17 | 0 | 5 | 5 | 5 |
Endoplasmic Reticulum | 79 | 80 | 80 | 58 | 73 | 90 | 92 | 95 | – | 87 | 29 | 69 | 57 | 83 | 40 | 4 | 4 | 3 | 27 | 38 | 30 | 29 | 52 | 49 |
Endosome | 10 | 3 | 35 | 9 | 10 | 18 | 17 | 20 | – | 22 | 10 | 3 | 3 | 2 | 4 | 37 | 38 | 6 | 46 | 48 | 53 | 36 | 33 | 45 |
Golgi | 159 | 38 | 174 | 117 | 150 | 98 | 155 | 162 | – | 93 | 35 | 61 | 45 | 100 | 84 | 52 | 32 | 47 | 174 | 80 | 185 | 101 | 144 | 214 |
Mitochondria | 19 | 32 | 57 | 58 | 47 | 154 | 183 | 257 | – | 288 | 139 | 154 | 1 | 2 | 0 | 30 | 17 | 6 | 4 | 4 | 4 | 8 | 7 | 8 |
Nucleus | 0 | 1 | 0 | 0 | 2 | 0 | 0 | 1 | – | 1 | 1 | 0 | 0 | 0 | 1 | 5 | 9 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 2 | 0 | 2 | 2 | – | 2 | 0 | 3 | 1 | 0 | 0 | 0 | 1 | 0 | 0 | 4 | 0 | 0 | 0 | 0 |
Nucleolus | 2 | 2 | 3 | 1 | 3 | 5 | 8 | 22 | – | 11 | 8 | 2 | 0 | 0 | 0 | 4 | 5 | 1 | 0 | 0 | 0 | 0 | 0 | 1 |
Peroxisomes | 0 | 0 | 4 | 1 | 1 | 0 | 2 | 0 | – | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 4 | 4 | 1 | 2 |
SpindlePole | 0 | 0 | 4 | 2 | 0 | 0 | 0 | 0 | – | 0 | 1 | 0 | 0 | 0 | 0 | 6 | 10 | 5 | 0 | 1 | 2 | 0 | 2 | 1 |
Vac/Vac Membrane | 42 | 7 | 20 | 0 | 14 | 13 | 17 | 16 | – | 14 | 4 | 2 | 4 | 11 | 13 | 30 | 23 | 18 | 3 | 22 | 6 | 7 | 7 | 9 |
Unique Cell Count | 263 | 198 | 326 | 193 | 267 | 322 | 408 | 513 | 458 | 230 | 271 | 112 | 226 | 175 | 185 | 139 | 89 | 299 | 257 | 375 | 244 | 318 | 392 | |
Labelled Cell Count | 388 | 276 | 481 | 315 | 401 | 483 | 609 | 734 | 656 | 327 | 396 | 169 | 306 | 222 | 247 | 193 | 126 | 299 | 257 | 375 | 244 | 318 | 392 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 17.4 | 14.6 | 14.3 | 13.9 | 15.8 | 13.9 | 14.5 | 13.7 | – | 13.4 | 13.0 | 14.4 | 15.2 | 16.2 | 17.1 | 11.1 | 11.0 | 16.2 | 15.9 | 16.6 | 18.0 |
Std Deviation (1e-4) | 4.2 | 3.7 | 3.2 | 3.1 | 4.0 | 3.7 | 4.2 | 3.5 | – | 4.6 | 4.6 | 5.2 | 3.5 | 3.6 | 4.5 | 3.5 | 3.8 | 5.4 | 2.8 | 3.6 | 4.2 |
Intensity Change (Log2) | – | – | – | -0.03 | 0.14 | -0.04 | 0.02 | -0.06 | – | -0.09 | -0.14 | 0.01 | 0.09 | 0.19 | 0.26 | -0.36 | -0.37 | 0.18 | 0.15 | 0.22 | 0.34 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0.4 | -1.1 | -2.1 | -1.8 | -1.8 | – | -1.2 | 0.1 | -0.7 | 0.3 | 0.7 | -1.8 | -0.2 | -1.4 | -0.8 |
Bud | -1.2 | 0.9 | 2.2 | 2.0 | 1.5 | – | 1.6 | 3.2 | 2.0 | 0 | 0 | 0 | -0.8 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 1.6 | 2.7 | 1.4 | 0.8 | 0.6 | – | -0.6 | 1.6 | 1.1 | 5.2 | 5.2 | 6.2 | 4.4 | 4.0 | 4.9 |
Endoplasmic Reticulum | 1.4 | 0.8 | 1.0 | -0.6 | -2.1 | – | -1.9 | -3.5 | 0.3 | 5.2 | 3.1 | -0.4 | -6.6 | -5.6 | -4.4 |
Endosome | -2.4 | -3.2 | -2.4 | -3.4 | -3.9 | – | -3.2 | -2.7 | -4.8 | -2.6 | -4.6 | -3.4 | 2.9 | 4.5 | -1.1 |
Golgi | 1.6 | 0.7 | -5.9 | -4.2 | -6.3 | – | -9.6 | -9.1 | -7.7 | -2.4 | -2.1 | -1.1 | -5.5 | -6.0 | -0.1 |
Mitochondria | 3.3 | 0 | 8.2 | 7.9 | 9.5 | – | 12.6 | 10.4 | 10.0 | -4.5 | -6.2 | -5.9 | -0.4 | -1.4 | -2.5 |
Nucleus | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 2.8 | – | 1.5 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | -3.5 | -0.5 | -1.2 | -1.2 | -2.1 | – | -2.1 | -2.5 | -3.5 | -1.0 | -0.6 | 0.6 | 3.7 | 3.5 | 4.1 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Actin | 0.1557 | 0.0989 | 0.0602 | 0.0613 | 0.0717 | 0.084 | 0.1405 | 0.0366 | 0.0452 | 0.0664 | 0.0411 | 0.0397 | – | – | – | – | – | – |
Bud | 0.0055 | 0.0024 | 0.0171 | 0.0052 | 0.0003 | 0.0017 | 0.0005 | 0.0005 | 0.0146 | 0.0007 | 0.0012 | 0.0002 | – | – | – | – | – | – |
Bud Neck | 0.0041 | 0.0015 | 0.0017 | 0.0023 | 0.0014 | 0.0439 | 0.0013 | 0.0004 | 0.0012 | 0.0008 | 0.0009 | 0.0137 | – | – | – | – | – | – |
Bud Periphery | 0.0085 | 0.0017 | 0.0195 | 0.0061 | 0.0002 | 0.0009 | 0.0007 | 0.0002 | 0.0131 | 0.0004 | 0.0019 | 0.0002 | – | – | – | – | – | – |
Bud Site | 0.0115 | 0.0187 | 0.0119 | 0.0019 | 0.0003 | 0.0005 | 0.0024 | 0.0103 | 0.0075 | 0.0063 | 0.0006 | 0.0002 | – | – | – | – | – | – |
Cell Periphery | 0.0062 | 0.0008 | 0.0012 | 0.001 | 0.0002 | 0.0005 | 0.0006 | 0.0002 | 0.0005 | 0.0002 | 0.0008 | 0.0015 | – | – | – | – | – | – |
Cytoplasm | 0.1053 | 0.0477 | 0.0583 | 0.0254 | 0.017 | 0.0807 | 0.0101 | 0.0262 | 0.0196 | 0.0763 | 0.0308 | 0.0144 | – | – | – | – | – | – |
Cytoplasmic Foci | 0.0338 | 0.0619 | 0.0653 | 0.0767 | 0.1276 | 0.0493 | 0.0375 | 0.0424 | 0.0315 | 0.0311 | 0.0328 | 0.029 | – | – | – | – | – | – |
Eisosomes | 0.0013 | 0.0002 | 0.0001 | 0.0002 | 0.0001 | 0.0002 | 0.002 | 0 | 0.0002 | 0.0002 | 0.0001 | 0.0002 | – | – | – | – | – | – |
Endoplasmic Reticulum | 0.0317 | 0.003 | 0.0107 | 0.0043 | 0.0023 | 0.0096 | 0.0231 | 0.005 | 0.0057 | 0.0033 | 0.0117 | 0.0037 | – | – | – | – | – | – |
Endosome | 0.1648 | 0.2452 | 0.27 | 0.238 | 0.1862 | 0.1331 | 0.1359 | 0.2001 | 0.1861 | 0.2188 | 0.1859 | 0.106 | – | – | – | – | – | – |
Golgi | 0.356 | 0.4735 | 0.4051 | 0.459 | 0.5596 | 0.5528 | 0.5937 | 0.6495 | 0.6446 | 0.5583 | 0.6012 | 0.7697 | – | – | – | – | – | – |
Lipid Particles | 0.0255 | 0.0213 | 0.0178 | 0.0653 | 0.0246 | 0.0073 | 0.015 | 0.015 | 0.0061 | 0.0153 | 0.0219 | 0.0084 | – | – | – | – | – | – |
Mitochondria | 0.0079 | 0.0128 | 0.0159 | 0.0058 | 0.0029 | 0.0146 | 0.0111 | 0.0067 | 0.0033 | 0.003 | 0.0038 | 0.0081 | – | – | – | – | – | – |
None | 0.0058 | 0.0001 | 0.0011 | 0.0002 | 0.0001 | 0.0009 | 0.001 | 0.0001 | 0.0004 | 0.0012 | 0.0012 | 0.0001 | – | – | – | – | – | – |
Nuclear Periphery | 0.0339 | 0.0005 | 0.0032 | 0.0038 | 0.0002 | 0.0063 | 0.0128 | 0.0007 | 0.0022 | 0.0019 | 0.0164 | 0.0002 | – | – | – | – | – | – |
Nucleolus | 0.0006 | 0.0003 | 0.0002 | 0.0002 | 0 | 0.0007 | 0.0001 | 0 | 0.0001 | 0.0001 | 0.0006 | 0 | – | – | – | – | – | – |
Nucleus | 0.0101 | 0.0002 | 0.0007 | 0.0004 | 0.0001 | 0.0023 | 0.001 | 0.0001 | 0.0003 | 0.0008 | 0.0016 | 0.0001 | – | – | – | – | – | – |
Peroxisomes | 0.005 | 0.0013 | 0.0056 | 0.002 | 0.0018 | 0.0011 | 0.0049 | 0.0011 | 0.0016 | 0.0046 | 0.0314 | 0.0006 | – | – | – | – | – | – |
Punctate Nuclear | 0.0181 | 0.0002 | 0.0011 | 0.0008 | 0.0004 | 0.0008 | 0.0016 | 0.0001 | 0.0008 | 0.0015 | 0.0017 | 0.0001 | – | – | – | – | – | – |
Vacuole | 0.0039 | 0.0041 | 0.0174 | 0.0143 | 0.0013 | 0.0039 | 0.0022 | 0.0027 | 0.0062 | 0.0038 | 0.0046 | 0.0016 | – | – | – | – | – | – |
Vacuole Periphery | 0.0048 | 0.0037 | 0.0156 | 0.0259 | 0.0018 | 0.0048 | 0.0022 | 0.0022 | 0.0093 | 0.0048 | 0.0078 | 0.0023 | – | – | – | – | – | – |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 59.8465 | 43.3191 | 44.0382 | 54.4851 | 48.6923 | 51.8221 | 57.0494 | 53.8028 | 62.1102 | 61.0584 |
Translational Efficiency | 1.7945 | 1.9787 | 1.5592 | 1.3062 | 1.5435 | 2.4248 | 1.6694 | 1.4271 | 1.3082 | 1.4575 |
Dataset | Hit |
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Protein Concentration | – |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✔ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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