ORF
Human Ortholog
Description Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.96 – 0.91 0.7 0.47 0.48 0.32 0.26 0.27 0.21 0.21 0.16 0.94 0.97 0.97 0.85 0.85 0.83 0.68 0.46 0.68 0.63 0.66 0.75
Endoplasmic Reticulum 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0 0 0 0 0 0
Endosome 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 – 0 0 0.13 0.1 0.17 0.19 0.14 0.15 0.12 0.13 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 – 0 0.05 0.1 0.17 0.22 0.22 0.33 0.29 0.35 0.32 0 0 0 0.14 0 0.05 0.26 0.44 0.22 0.31 0.21 0.11
Nuclear Periphery 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 – 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 – 0.15 0.36 0.44 0.38 0.41 0.42 0.36 0.37 0.38 0.39 0.07 0.07 0.06 0.2 0.24 0.31 0 0.05 0 0 0.05 0.05
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 – 0 0 0 1 0 2 3 2 6 10 0 0 0 0 0 0 0 0 1 0 0 1
Bud 0 – 1 0 0 0 0 3 0 0 3 5 0 0 0 0 0 0 0 0 2 2 18 9
Bud Neck 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 1 1
Bud Site 0 – 0 0 0 0 0 0 1 0 0 1 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 – 1 0 1 2 2 1 0 3 1 1 0 1 1 1 0 1 0 0 0 0 0 0
Cytoplasm 23 – 253 186 164 187 177 186 119 95 86 101 246 583 630 88 190 147 46 14 189 135 358 409
Endoplasmic Reticulum 0 – 4 3 6 6 5 10 4 7 1 2 5 1 3 3 8 12 0 0 1 0 1 7
Endosome 0 – 0 0 0 0 0 0 1 1 0 0 0 0 0 0 0 1 0 0 1 0 3 5
Golgi 0 – 1 2 46 38 97 136 64 67 50 82 0 1 2 1 8 5 0 0 0 0 0 0
Mitochondria 0 – 0 0 1 0 1 0 5 5 4 3 1 0 0 0 1 0 0 0 0 0 0 0
Nucleus 0 – 3 14 36 68 121 161 150 135 145 200 1 0 0 15 7 8 18 13 60 65 117 60
Nuclear Periphery 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 5 1 6 8
Nucleolus 0 – 0 1 0 1 3 29 7 10 9 31 0 0 0 0 0 0 0 0 0 0 1 1
Peroxisomes 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 2 5
Vac/Vac Membrane 1 – 42 95 154 148 227 301 160 172 158 238 17 42 39 21 53 54 2 1 9 7 29 28
Unique Cell Count 24 279 264 350 389 556 723 448 461 417 617 261 602 652 104 224 177 69 31 280 215 545 544
Labelled Cell Count 24 305 301 408 451 633 829 514 497 463 674 270 628 675 129 267 228 69 31 280 215 545 544


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 28.7 – 30.8 29.0 34.7 29.9 29.0 27.2 23.9 22.4 21.5 21.7 37.0 39.0 37.5 30.9 36.4 40.6 33.8 35.0 34.9
Std Deviation (1e-4) 5.2 – 7.0 9.8 7.1 6.3 5.8 5.6 5.3 4.7 5.1 4.6 8.3 8.6 8.2 10.4 12.6 13.2 6.3 7.4 8.0
Intensity Change (Log2) – – – -0.08 0.17 -0.04 -0.09 -0.18 -0.37 -0.46 -0.52 -0.51 0.26 0.34 0.29 0.0 0.24 0.4 0.13 0.18 0.18


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm -6.0 -11.6 -11.5 -16.0 -18.6 -16.8 -18.5 -18.1 -21.1 1.6 3.9 3.8 -1.7 -2.0 -2.4
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 1.6 3.0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 6.1 5.1 7.2 7.6 6.4 6.5 5.8 6.2 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 2.8 4.8 6.8 7.9 8.1 10.4 9.5 10.6 10.4 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 3.4 0 0 0 3.8 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 5.6 7.8 6.5 7.5 7.9 6.0 6.5 6.5 7.0 -3.2 -3.8 -4.5 1.2 2.5 3.9

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 35.6276 39.2815 36.3691 36.646 32.8206 36.64 27.027 37.0188 33.3663 30.3439 29.4166 32.915 34.7988 41.503 36.5496 35.7199 37.1008 39.1445
Actin 0.0053 0.0001 0.0052 0.0003 0.0004 0.0001 0.01 0.0007 0.0132 0.0004 0.0126 0.0002 0.0005 0.0001 0.0055 0.002 0.0001 0.0002
Bud 0.0001 0 0.0002 0.0001 0.0006 0 0.0008 0.0001 0.0003 0.0001 0.0003 0.0001 0.0001 0 0.0001 0.0001 0.0001 0.0001
Bud Neck 0.0002 0.0003 0.0003 0.0003 0.0008 0.0006 0.0007 0.0003 0.0004 0.0002 0.0012 0.0007 0.0002 0.0002 0.0003 0.0003 0.0003 0.0006
Bud Periphery 0.0001 0 0.0003 0 0.0008 0 0.0009 0 0.0002 0.0001 0.0003 0 0.0001 0 0.0001 0 0 0
Bud Site 0.0002 0 0.0011 0 0.0003 0 0.003 0.0003 0.0016 0.0001 0.0024 0 0.0003 0 0.0002 0.0001 0 0
Cell Periphery 0.0001 0 0.0001 0 0.0006 0 0.0004 0.0001 0.0001 0 0.0002 0 0 0 0 0 0 0
Cytoplasm 0.1587 0.3296 0.1617 0.1762 0.2269 0.2531 0.2194 0.3924 0.2533 0.2763 0.219 0.3877 0.288 0.4628 0.3023 0.3734 0.4313 0.4374
Cytoplasmic Foci 0.0045 0 0.0002 0.0003 0.0015 0.0001 0.0355 0.0008 0.008 0.0048 0.0267 0.0004 0.0066 0.0001 0.004 0.0018 0 0.0001
Eisosomes 0 0 0.0001 0 0.0001 0 0.0004 0 0.0002 0 0.0002 0 0 0 0.0001 0 0 0
Endoplasmic Reticulum 0.0007 0.0004 0.0006 0.0002 0.0016 0.0012 0.0092 0.0007 0.002 0.0011 0.0025 0.0011 0.0008 0.0002 0.0008 0.0001 0.0002 0.0005
Endosome 0.0015 0 0.0014 0.0001 0.0009 0.0002 0.0348 0.0008 0.0075 0.0208 0.0266 0.0007 0.0053 0 0.0012 0.0005 0 0.0001
Golgi 0.0007 0 0.0002 0 0.0001 0 0.006 0.0005 0.0017 0.0035 0.0299 0 0.0007 0 0.0005 0.0018 0 0
Lipid Particles 0.0033 0 0.0002 0 0.0013 0 0.0225 0.0005 0.0013 0.0042 0.0245 0 0.0067 0 0.0032 0.0111 0 0
Mitochondria 0.0072 0.0001 0.0004 0.0001 0.019 0.0001 0.0064 0.0123 0.0009 0.0102 0.0111 0.0003 0.0008 0.0002 0.002 0.0015 0.0001 0.0002
None 0.0007 0.0001 0.0015 0.0001 0.0014 0.0001 0.025 0.0005 0.0094 0.0003 0.0017 0.0002 0.0013 0.0001 0.001 0.0001 0.0001 0.0001
Nuclear Periphery 0.0548 0.0329 0.0406 0.0417 0.0486 0.0529 0.0411 0.0278 0.0338 0.0258 0.0222 0.0375 0.0445 0.0251 0.0269 0.0284 0.0216 0.0329
Nucleolus 0.0031 0.0003 0.0009 0.0004 0.012 0.0002 0.0015 0.0006 0.0003 0.0002 0.0003 0.0001 0.0005 0.0001 0.0005 0.0001 0.0001 0.0002
Nucleus 0.7491 0.6346 0.7832 0.7777 0.6697 0.689 0.5609 0.5559 0.6599 0.6462 0.6029 0.5688 0.6363 0.51 0.6469 0.5587 0.5454 0.5259
Peroxisomes 0.0019 0 0.0001 0 0.0004 0 0.0045 0.0002 0.001 0.0006 0.0112 0 0.0022 0 0.0008 0.0184 0 0
Punctate Nuclear 0.0024 0.0006 0.0007 0.0012 0.0012 0.0006 0.0071 0.0043 0.0018 0.0011 0.0022 0.001 0.0035 0.0007 0.0018 0.0013 0.0006 0.0009
Vacuole 0.0028 0.0007 0.0007 0.0007 0.0065 0.0014 0.0076 0.001 0.0024 0.0022 0.0013 0.0006 0.0009 0.0002 0.0012 0.0002 0.0002 0.0005
Vacuole Periphery 0.0025 0.0002 0.0004 0.0004 0.0052 0.0004 0.002 0.0003 0.0007 0.0016 0.0007 0.0005 0.0005 0.0001 0.0006 0.0002 0.0001 0.0003

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 62.7954 53.4621 39.6712 59.1026 67.8475 71.7264 72.9749 72.0555 70.4637 57.7086
Translational Efficiency 1.9209 2.3669 2.7285 2.3798 1.5761 2.3054 1.5916 1.948 2.06 1.982

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
733 1002 873 1286 1459 2181 1755 300 2192 3183 2628 1586

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 2388.86 2504.24 2468.80 2633.33 2384.25 2416.96 2522.51 2857.17 2385.79 2444.44 2504.67 2675.67
Standard Deviation 557.16 490.60 534.84 592.29 512.83 429.64 489.13 565.82 528.07 451.54 505.41 593.88
Intensity Change Log 2 — 0.068051 0.047488 0.140566 — 0.019658 0.081324 0.261051 — 0.044081 0.064489 0.202008

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000543 0.000326 0.000354 0.000784 0.000219 0.000139 0.000270 0.000375 0.000327 0.000198 0.000298 0.000706
Bud Neck 0.009384 0.003886 0.005290 0.010796 0.002296 0.003328 0.005156 0.004664 0.004666 0.003504 0.005201 0.009636
Bud Site 0.006495 0.001208 0.002874 0.012344 0.000522 0.001827 0.002470 0.003805 0.002519 0.001632 0.002604 0.010729
Cell Periphery 0.000841 0.000211 0.000281 0.000309 0.000235 0.000088 0.000192 0.000371 0.000437 0.000127 0.000221 0.000321
Cytoplasm 0.188291* 0.117090* 0.198426* 0.194832* 0.159695* 0.135440* 0.113489* 0.123766* 0.169257* 0.129664* 0.141705* 0.181389*
Cytoplasmic Foci 0.004568 0.001851 0.001330 0.009116 0.000998 0.000458 0.000991 0.002100 0.002192 0.000896 0.001104 0.007789
Eisosomes 0.000008 0.000004 0.000008 0.000009 0.000003 0.000002 0.000006 0.000006 0.000005 0.000003 0.000006 0.000009
Endoplasmic Reticulum 0.000881 0.000293 0.000527 0.000856 0.000278 0.000272 0.000306 0.000275 0.000480 0.000278 0.000380 0.000746
Endosome 0.000169 0.000462 0.000215 0.009955 0.000040 0.000183 0.002038 0.000080 0.000083 0.000271 0.001432 0.008087
Golgi 0.000379 0.001091 0.005370 0.015951 0.000100 0.000200 0.000595 0.000107 0.000193 0.000480 0.002181 0.012954
Lipid Particles 0.000156 0.000152 0.000274 0.001547 0.000062 0.000044 0.000140 0.000257 0.000093 0.000078 0.000184 0.001303
Mitochondria 0.001763 0.000593 0.001008 0.008995 0.000507 0.000422 0.001364 0.001070 0.000927 0.000476 0.001246 0.007496
Mitotic Spindle 0.002662 0.000411 0.003226 0.104789* 0.000030 0.001001 0.013394 0.014142 0.000910 0.000816 0.010016 0.087642
None 0.008572 0.005892 0.010680 0.023466 0.005172 0.006772 0.009857 0.020089 0.006309 0.006495 0.010131 0.022827
Nuclear Periphery 0.000399 0.000240 0.000715 0.001648 0.000194 0.000256 0.000721 0.000677 0.000263 0.000251 0.000719 0.001464
Nuclear Periphery Foci 0.000587 0.001527 0.000431 0.001552 0.000265 0.000223 0.000273 0.000328 0.000372 0.000634 0.000326 0.001320
Nucleolus 0.000390 0.000174 0.000703 0.001589 0.000084 0.000214 0.000500 0.000274 0.000186 0.000201 0.000567 0.001340
Nucleus 0.770022* 0.862175* 0.754491* 0.569563* 0.827905* 0.847347* 0.842467* 0.821190* 0.808549* 0.852015* 0.813242* 0.617159*
Peroxisomes 0.000035 0.000085 0.000026 0.000502 0.000006 0.000031 0.000114 0.000017 0.000016 0.000048 0.000085 0.000410
Vacuole 0.003066 0.002011 0.013693 0.020011 0.001351 0.001492 0.004903 0.006353 0.001925 0.001656 0.007823 0.017427
Vacuole Periphery 0.000789 0.000318 0.000080 0.011387 0.000038 0.000262 0.000753 0.000053 0.000289 0.000279 0.000529 0.009243

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin 3.02 1.21 1.79 -1.42 0.60 4.03 -7.41 -5.77 -7.79 -1.50 5.07 -4.68 -3.39 -6.72 -0.12
Bud Neck 3.81 3.16 -0.15 -4.51 -3.96 -1.03 -10.86 -7.58 -6.96 -2.72 2.45 -5.68 -7.66 -9.18 -3.22
Bud Site 6.01 4.13 1.66 -4.74 -2.54 -2.45 -7.73 -6.69 -4.17 -3.08 2.34 -0.83 -3.81 -5.22 -3.44
Cell Periphery 6.76 5.08 6.05 -2.19 1.85 8.40 -1.69 -4.55 -9.01 -3.67 9.62 3.55 2.78 -10.55 -0.99
Cytoplasm 8.61 3.96 4.46 -5.33 0.37 4.62 12.95 7.72 5.06 -0.90 8.78 12.42 5.52 -2.04 -5.74
Cytoplasmic Foci 3.91 4.57 3.27 -1.00 -1.31 3.09 0.48 -2.03 -3.71 -2.42 5.11 3.60 -0.44 -4.86 -3.54
Eisosomes 2.57 -2.38 -3.57 -10.32 -1.58 4.20 -13.32 -9.58 -10.49 -4.29 4.20 -10.46 -12.46 -18.22 -3.64
Endoplasmic Reticulum 1.99 0.61 1.25 -4.13 2.72 0.27 -9.19 -5.70 -5.82 0.40 2.01 -1.45 -0.47 -6.36 2.27
Endosome -1.01 -1.04 -2.64 -1.59 -2.52 -2.32 -2.17 -6.42 -0.25 1.69 -1.90 -2.24 -2.96 -2.28 -1.14
Golgi -1.05 -0.80 -1.82 -0.72 -0.24 -0.97 -2.18 -3.84 -0.42 0.78 -1.25 -1.09 -2.10 -1.58 -1.09
Lipid Particles -0.22 -6.29 -6.37 -6.11 -2.81 1.45 -7.56 -6.62 -7.10 -3.50 0.85 -9.80 -8.09 -8.33 -4.68
Mitochondria 7.06 1.97 -1.15 -3.73 -1.91 3.00 -22.30 -13.82 -14.48 0.72 7.55 -11.28 -3.44 -4.68 -1.38
Mitotic Spindle 2.26 -2.00 -7.74 -8.59 -6.65 -3.08 -5.71 -4.02 -3.73 -1.85 -0.21 -5.98 -9.14 -9.12 -6.43
None 2.74 -1.15 -7.01 -9.73 -6.71 -3.20 -8.55 -7.14 -6.07 -4.60 -0.44 -6.81 -11.37 -11.11 -8.28
Nuclear Periphery 1.05 -7.59 -10.59 -14.97 -5.90 -3.96 -23.22 -13.23 -12.56 -4.41 -0.26 -19.83 -16.70 -17.55 -7.27
Nuclear Periphery Foci -0.83 0.89 0.03 0.86 -2.02 0.95 -0.16 -1.28 -2.29 -1.32 -0.58 0.62 -1.62 -0.02 -3.11
Nucleolus 2.73 -0.74 -5.52 -7.55 -4.21 -2.15 -8.64 -6.72 -5.75 -1.85 0.08 -7.23 -9.04 -8.84 -3.96
Nucleus -9.73 -0.76 5.57 17.36 6.90 -3.54 -4.11 3.04 4.85 5.17 -8.77 -3.60 11.03 18.40 14.20
Peroxisomes -0.73 -0.88 -2.19 -0.26 -1.96 -1.68 -4.37 -6.27 -1.84 1.07 -1.36 -4.44 -3.32 -1.48 -0.99
Vacuole 0.72 -10.93 -16.48 -16.79 -5.27 -1.12 -12.71 -7.76 -7.67 -5.08 -0.25 -16.27 -18.68 -18.64 -10.72
Vacuole Periphery 1.94 3.41 -0.69 -1.60 -2.05 -1.48 -2.20 -8.43 1.00 1.60 0.18 0.63 -1.58 -1.60 -1.72
ORF
Human Ortholog
Description Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus
Localization
Cell Percentages cytoplasm (89%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-7

YBR090C

YBR090C


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Ybr090c-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available