Standard name
Human Ortholog
Description Constitutively expressed acid phosphatase similar to Pho5p; brought to the cell surface by transport vesicles; hydrolyzes thiamin phosphates in the periplasmic space, increasing cellular thiamin uptake; expression is repressed by thiamin
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.98 0.97 0.93 0.96 0.93 0.79 0.71 0.78 0.69 0.68 0.46 0.51 1.0 0.99 0.99 0.9 0.86 0.9 0.72 0.77 0.64 0.76 0.73 0.7
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.08 0.05 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.24 0 0.06 0.09 0.08 0.37 0.4 0.34 0.5 0.55 0.69 0.66 0 0 0 0 0 0 0.13 0.11 0.22 0.06 0.05 0.06
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.13 0.27 0.14 0 0 0 0.08 0.09 0.08
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 1 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 1 3 3 3 6 8
Bud 0 0 5 1 6 2 6 15 10 10 10 11 0 1 0 0 0 0 0 0 2 7 16 16
Bud Neck 0 0 1 1 1 1 1 10 5 2 0 2 0 0 0 1 0 1 0 0 0 2 2 7
Bud Site 0 0 0 0 0 1 2 0 2 2 1 1 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 1 0 1 1 2 2 0 0 0 0 1 1 2 0 0 1 1 0 0 1 2 4 5
Cytoplasm 45 194 169 190 246 271 285 421 217 237 121 180 387 820 765 409 228 237 67 154 117 222 419 321
Endoplasmic Reticulum 0 2 0 2 0 0 0 1 1 1 3 0 0 2 2 37 12 10 3 1 2 2 5 1
Endosome 0 0 0 0 1 0 0 2 0 0 0 0 0 0 1 8 3 3 1 3 1 0 6 3
Golgi 0 0 2 1 1 0 0 1 0 0 0 0 0 1 0 1 1 1 0 1 1 0 4 3
Mitochondria 11 5 11 17 21 129 159 185 156 191 179 234 0 1 0 12 0 0 12 22 39 18 31 28
Nucleus 0 1 0 1 0 1 1 2 2 2 1 0 1 0 1 0 0 2 0 0 0 0 0 0
Nuclear Periphery 0 1 0 1 1 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 1 1
Nucleolus 0 0 1 0 1 2 1 3 0 0 0 0 0 0 0 0 2 0 0 0 0 0 1 0
Peroxisomes 0 0 0 0 0 1 1 0 0 0 0 0 0 2 1 0 0 0 0 0 1 0 0 2
SpindlePole 0 0 1 0 1 5 13 21 9 5 5 7 0 0 0 0 0 0 1 1 2 3 10 9
Vac/Vac Membrane 0 1 2 0 3 1 4 2 1 2 2 5 0 1 1 61 71 36 2 4 3 24 53 37
Unique Cell Count 46 201 182 198 264 345 400 541 315 348 261 353 388 828 770 452 266 263 94 199 183 294 574 460
Labelled Cell Count 56 205 193 215 283 416 475 663 403 454 322 441 389 830 771 529 318 291 94 199 183 294 574 460


Vacuole

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 3.2 3.8 3.5 3.4 3.7 2.9 2.6 2.9 2.7 2.6 2.6 2.4 3.9 4.0 4.1 4.9 5.9 5.6 3.8 4.3 4.8
Std Deviation (1e-4) 0.5 1.0 1.4 1.4 2.1 1.1 1.1 1.0 1.1 1.6 0.9 1.1 1.3 1.4 1.5 1.2 1.5 1.2 1.1 1.9 2.0
Intensity Change (Log2) – – – -0.03 0.08 -0.28 -0.41 -0.27 -0.36 -0.45 -0.45 -0.53 0.16 0.18 0.22 0.5 0.75 0.69 0.13 0.29 0.46


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – 0 0 0
Bud Neck – – – – – – – – – – – – 0 0 0
Bud Site – – – – – – – – – – – – 0 0 0
Cell Periphery – – – – – – – – – – – – 0 0 0
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – 0 0 0
Endosome – – – – – – – – – – – – 0 0 0
Golgi – – – – – – – – – – – – 0 0 0
Mitochondria – – – – – – – – – – – – 0 0 0
Nuclear Periphery – – – – – – – – – – – – 0 0 0
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – 0 0 0
Peroxisomes – – – – – – – – – – – – 0 0 0
SpindlePole – – – – – – – – – – – – 0 0 0
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – 0 0 0
Cytoplasm – – – – – – – – – – – – 0 0 0
Nucleus – – – – – – – – – – – – 0 0 0
Vacuole – – – – – – – – – – – – 4.7 7.2 4.7

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration -1.9677 -1.1532 -1.602 -1.7596 -1.9393 -1.4173 0.8915 1.015 1.1961 0.59 1.1089 1.1264 1.1417 2.0008 1.7199 1.1129 1.4298 1.9172
Actin 0.0059 0.0019 0.0027 0.0002 0.0003 0.0011 0.0271 0.0003 0.0329 0.0358 0.0169 0.0042 0.0264 0.0001 0.0256 0.0011 0.0003 0.0305
Bud 0.0036 0.0003 0.0011 0 0 0.0018 0.0013 0.0008 0.0018 0.0027 0.0019 0.0007 0.0003 0.0003 0.0002 0.0023 0.0014 0.0007
Bud Neck 0.0023 0.0001 0.0003 0.0001 0 0.0074 0.0016 0.0018 0.0012 0.0002 0.001 0.0017 0.0012 0.0002 0.0005 0.0004 0.0076 0.001
Bud Periphery 0.0013 0.0006 0.0011 0.0002 0.0001 0.0031 0.0036 0.0008 0.0042 0.0176 0.0098 0.0025 0.0004 0.0005 0.0004 0.0044 0.0014 0.0015
Bud Site 0.0116 0.0003 0.0035 0 0 0.0001 0.01 0.0096 0.0047 0.0003 0.0037 0.0002 0.0014 0.0293 0.0007 0.0005 0.0315 0.0005
Cell Periphery 0.0006 0.0001 0.0001 0.0002 0 0.0001 0.0005 0.0001 0.0003 0.0001 0.0001 0.0001 0.0002 0.0002 0.0002 0.0002 0.0004 0.0001
Cytoplasm 0.0234 0.0548 0.0224 0.0263 0.0269 0.0512 0.0202 0.0106 0.0076 0.009 0.0102 0.0211 0.0143 0.0147 0.0126 0.0226 0.0103 0.0152
Cytoplasmic Foci 0.0311 0.004 0.0076 0.0074 0.0014 0.022 0.013 0.0022 0.0212 0.0236 0.0479 0.016 0.0227 0.0017 0.0041 0.0047 0.0148 0.0019
Eisosomes 0.002 0.0005 0.0012 0.0003 0.0002 0.0002 0.0007 0.0002 0.0004 0.0002 0.0002 0.0001 0.0008 0.0003 0.001 0.0002 0.0003 0.0004
Endoplasmic Reticulum 0.0012 0.0003 0.0004 0.0004 0.0001 0.0011 0.0035 0.0002 0.0012 0.0008 0.0007 0.0003 0.0039 0.0003 0.0012 0.0007 0.0002 0.0007
Endosome 0.0099 0.0004 0.0014 0.0038 0 0.0119 0.0057 0.0001 0.0224 0.0184 0.0327 0.0062 0.0113 0.0002 0.0055 0.0004 0.0001 0.0021
Golgi 0.0017 0.0001 0.0006 0.0001 0 0.0007 0.0034 0 0.0107 0.0045 0.0268 0.0028 0.0038 0 0.0015 0.0001 0.0001 0.0018
Lipid Particles 0.0079 0.0002 0.0044 0.0004 0.0001 0.0023 0.0127 0.0002 0.0087 0.0055 0.0115 0.0011 0.0118 0.0002 0.0023 0.0006 0.0002 0.0018
Mitochondria 0.003 0.0004 0.0009 0.0003 0.0001 0.0016 0.0043 0.0003 0.0048 0.0236 0.0174 0.0119 0.0025 0.0002 0.0014 0.0005 0.0002 0.0012
None 0.8691 0.9337 0.9403 0.9552 0.9696 0.8868 0.8674 0.9706 0.8577 0.8317 0.8015 0.9207 0.8452 0.9508 0.9271 0.9532 0.9281 0.9359
Nuclear Periphery 0.0016 0.0002 0.0007 0.0004 0.0001 0.0016 0.0011 0.0002 0.0034 0.002 0.0008 0.0005 0.0196 0.0001 0.0023 0.0008 0.0001 0.0017
Nucleolus 0.0032 0.0001 0.0008 0.0003 0.0001 0.0004 0.0008 0.0002 0.0018 0.0005 0.0003 0.0007 0.0012 0.0001 0.0005 0.0009 0.0009 0.0002
Nucleus 0.0027 0.0003 0.0028 0.0004 0.0002 0.0008 0.001 0.0004 0.0017 0.0007 0.0003 0.0008 0.0104 0.0003 0.0007 0.0016 0.0004 0.0004
Peroxisomes 0.004 0.0006 0.0029 0.0001 0.0001 0.001 0.018 0.0001 0.0066 0.0168 0.0131 0.0025 0.0068 0.0001 0.0004 0.0003 0.0004 0.0009
Punctate Nuclear 0.0115 0.0006 0.0045 0.0018 0.0007 0.0018 0.0023 0.001 0.0013 0.0006 0.0008 0.0027 0.0143 0.0002 0.0094 0.0031 0.0013 0.0009
Vacuole 0.0019 0.0002 0.0004 0.0019 0 0.0024 0.0015 0.0001 0.0043 0.0039 0.0015 0.0026 0.0008 0.0003 0.0015 0.001 0.0002 0.0002
Vacuole Periphery 0.0006 0 0.0001 0.0002 0 0.0006 0.0003 0 0.001 0.0015 0.001 0.0007 0.0007 0 0.0007 0.0002 0 0.0002

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 104.9177 132.0789 645.9158 423.3903 74.3837 123.3181 131.9465 284.3048 266.528 78.2584
Translational Efficiency 1.0349 0.7775 1.0498 0.9738 1.0083 0.7749 0.9441 0.9816 0.9678 0.9042

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✔
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1072 1504 304 819 1294 1182 2068 1035 2366 2686 2372 1854

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 603.93 680.48 1052.76 824.04 657.20 683.19 963.42 904.65 633.06 681.67 974.87 869.04
Standard Deviation 75.26 95.40 195.77 110.12 68.21 88.64 159.99 167.35 76.25 92.50 167.69 150.31
Intensity Change Log 2 — 0.172171 0.801723 0.448333 — 0.055954 0.551832 0.461027 — 0.112778 0.676907 0.454962

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000056 0.000293 0.001253 0.000494 0.000032 0.000349 0.000446 0.005371 0.000043 0.000318 0.000550 0.003216
Bud Neck 0.003092 0.034854 0.020934 0.024241 0.005330 0.051904 0.030620 0.024806 0.004316 0.042357 0.029378 0.024557
Bud Site 0.001402 0.013001 0.067604 0.040544 0.001051 0.017488 0.023439 0.141213* 0.001210 0.014975 0.029100 0.096742
Cell Periphery 0.000182 0.000112 0.003781 0.000170 0.000143 0.000144 0.000315 0.001141 0.000161 0.000126 0.000759 0.000712
Cytoplasm 0.536156* 0.387762* 0.064985 0.157452* 0.519886* 0.344498* 0.114653* 0.200940* 0.527258* 0.368723* 0.108287* 0.181729*
Cytoplasmic Foci 0.092254 0.257705* 0.111883 0.087499 0.168913 0.272840* 0.040563 0.092943 0.134180 0.264365* 0.049704 0.090538
Eisosomes 0.000097 0.000126 0.000053 0.000039 0.000114 0.000182 0.000025 0.000097 0.000106 0.000151 0.000029 0.000071
Endoplasmic Reticulum 0.000289 0.000797 0.006125 0.001591 0.000319 0.000440 0.002354 0.002580 0.000305 0.000640 0.002837 0.002143
Endosome 0.001936 0.030082 0.217071* 0.199180 0.003852 0.040274 0.167468 0.162460 0.002984 0.034567 0.173825 0.178681
Golgi 0.000597 0.006158 0.016655 0.003468 0.000829 0.011216 0.002182 0.016528 0.000724 0.008384 0.004037 0.010759
Lipid Particles 0.003851 0.009260 0.012038 0.004031 0.007158 0.009710 0.005110 0.006356 0.005660 0.009458 0.005998 0.005329
Mitochondria 0.001372 0.019359 0.000249 0.005666 0.002545 0.021730 0.001004 0.004216 0.002013 0.020402 0.000907 0.004857
Mitotic Spindle 0.000586 0.005761 0.011935 0.023410 0.000046 0.011832 0.010181 0.029354 0.000291 0.008433 0.010406 0.026728
None 0.014234 0.005708 0.000130 0.000910 0.006168 0.002210 0.000683 0.001620 0.009823 0.004169 0.000612 0.001307
Nuclear Periphery 0.000331 0.000596 0.000499 0.000775 0.000476 0.000386 0.001210 0.000647 0.000410 0.000504 0.001119 0.000704
Nuclear Periphery Foci 0.000419 0.000352 0.013469 0.001799 0.000162 0.000246 0.003235 0.002916 0.000278 0.000306 0.004547 0.002422
Nucleolus 0.001051 0.002862 0.000898 0.002352 0.000945 0.001814 0.003488 0.000748 0.000993 0.002401 0.003156 0.001457
Nucleus 0.332509* 0.118683 0.016359 0.029493 0.259782* 0.097738 0.035213 0.035868 0.292734* 0.109466 0.032796 0.033052
Peroxisomes 0.000870 0.018452 0.009753 0.015205 0.002129 0.025518 0.003964 0.016977 0.001559 0.021561 0.004706 0.016195
Vacuole 0.008377 0.084646 0.410131* 0.397000* 0.019577 0.086832 0.545175* 0.245743* 0.014502 0.085608 0.527868* 0.312560*
Vacuole Periphery 0.000340 0.003433 0.014195 0.004682 0.000542 0.002647 0.008671 0.007476 0.000450 0.003087 0.009379 0.006242

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -6.11 -2.67 -3.52 -1.21 1.87 -8.27 -1.90 -4.05 -3.68 -3.57 -10.54 -2.66 -4.35 -3.78 -3.21
Bud Neck -14.96 -5.82 -9.19 5.14 0.06 -14.74 -13.34 -9.94 9.29 4.35 -20.66 -15.12 -13.56 10.33 4.38
Bud Site -9.46 -8.74 -9.96 -5.87 4.34 -10.55 -15.80 -17.62 -14.56 -14.00 -14.34 -17.20 -19.44 -15.48 -11.75
Cell Periphery 3.03 -1.34 1.76 -0.32 1.37 0.17 -2.19 -5.91 -5.92 -4.93 3.03 -1.58 -5.41 -5.91 0.13
Cytoplasm 14.11 37.97 29.86 16.86 -9.07 16.61 43.53 26.04 9.09 -8.76 21.33 56.36 39.08 18.19 -9.96
Cytoplasmic Foci -24.62 -1.73 5.40 27.65 4.28 -12.69 26.07 16.26 26.69 -8.25 -24.17 23.57 16.11 38.25 -6.52
Eisosomes -3.54 4.08 7.87 13.71 1.06 -5.86 15.77 2.31 6.02 -4.22 -6.47 17.99 4.77 9.14 -4.83
Endoplasmic Reticulum -3.97 -2.81 -8.09 -5.22 2.06 -2.59 -8.44 -4.85 -4.56 -0.29 -4.73 -7.18 -7.35 -5.93 1.43
Endosome -15.24 -15.31 -16.22 -12.10 5.16 -14.51 -26.83* -16.19 -9.58 3.88 -20.90 -30.55 -22.89 -15.56 4.67
Golgi -7.26 -6.77 -2.67 4.92 6.05 -7.20 -3.59 -5.95 0.61 -5.37 -10.00 -7.41 -6.40 1.66 -3.22
Lipid Particles -5.23 -2.69 0.65 6.46 2.89 -2.20 2.88 2.55 4.23 -0.54 -4.89 0.25 2.41 7.17 1.54
Mitochondria -8.67 2.07 -1.32 6.49 -2.22 -8.19 2.58 -0.40 7.96 -2.75 -11.84 2.53 -1.32 10.36 -2.95
Mitotic Spindle -4.22 -3.37 -7.05 -5.59 -2.68 -5.49 -7.41 -9.31 -5.40 -5.74 -7.02 -7.91 -11.65 -8.08 -6.59
None 4.56 8.58 7.31 3.54 -2.50 4.75 7.06 5.22 0.16 -4.55 5.61 10.53 8.88 3.44 -3.60
Nuclear Periphery -1.98 -2.23 -8.55 -5.58 -5.82 1.28 -9.52 -6.35 -7.03 1.09 -1.22 -10.62 -10.48 -8.13 -0.74
Nuclear Periphery Foci 0.48 -4.77 -5.80 -6.89 4.18 -2.28 -6.28 -4.90 -4.77 -0.51 -0.37 -7.57 -6.49 -6.51 2.40
Nucleolus -4.26 -0.25 -3.05 0.16 -2.63 -3.80 -5.67 -0.07 3.50 5.56 -5.67 -5.40 -2.95 1.98 3.19
Nucleus 24.29 34.16 31.25 9.94 -5.28 22.07 32.59 26.63 5.45 -2.86 32.03 46.40 40.54 11.03 -3.80
Peroxisomes -14.49 -4.51 -7.48 5.38 -0.30 -12.24 -3.04 -7.34 6.23 -6.39 -18.49 -6.17 -10.35 8.01 -7.25
Vacuole -23.89 -22.72 -39.59 -31.13 -1.73 -16.50 -72.28* -29.26 -20.80 18.73 -27.98 -76.02* -47.10 -35.53 14.78
Vacuole Periphery -6.82 -7.01 -4.45 0.80 5.41 -7.50 -11.92 -4.79 -2.29 2.21 -9.34 -14.00 -6.29 -1.36 5.17
Standard name
Human Ortholog
Description Constitutively expressed acid phosphatase similar to Pho5p; brought to the cell surface by transport vesicles; hydrolyzes thiamin phosphates in the periplasmic space, increasing cellular thiamin uptake; expression is repressed by thiamin
Localization
Cell Percentages cytoplasm (28%), nucleus (3%), mixed (52%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Pho3

Pho3


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Pho3-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available