Standard name
Human Ortholog
Description Component of the histone deacetylase Rpd3L complex; possibly involved in cell fusion and invasive growth; relocalizes to the cytosol in response to hypoxia
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0.05 0 0.05 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0.06 0.06 0.05 0 0 0 0 0 0 0.11 0.17 0.16 0.15 0.09 0.14 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.07 0.15 0 0.07 0.07 0.15 0.25 0.17 0.18 0.26 0.26 0.31 0 0 0 0.06 0.07 0 0 0 0 0 0 0
Nucleus 0.88 0.9 0.88 0.83 0.81 0.74 0.69 0.63 0.67 0.63 0.58 0.56 0.87 0.81 0.78 0.72 0.8 0.76 0.8 0.83 0.74 0.81 0.78 0.67
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.35 0.18 0.18 0.17 0.19 0.32 0.33 0.46 0.4 0.46 0.52 0.46 0.08 0.06 0.06 0 0 0 0.14 0.12 0.16 0.09 0.12 0.17
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0.06 0.08 0 0 0 0 0 0.07
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 1 0 0 0
Bud 0 3 0 1 1 0 5 4 4 4 4 2 0 1 0 0 2 0 2 4 2 1 4 3
Bud Neck 0 0 0 0 1 0 1 0 7 2 4 3 0 0 0 0 2 0 1 1 1 0 0 1
Bud Site 1 1 0 2 1 9 13 14 30 26 29 21 0 1 1 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 1 0 1 1 0 0 0 0 0 0 0 0
Cytoplasm 4 4 11 15 13 21 21 22 15 15 11 16 47 110 94 42 27 42 0 0 0 6 4 4
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 8 9 6 0 0 1 0 0 0
Endosome 0 0 3 0 2 0 0 0 0 0 0 0 1 1 5 8 5 9 4 2 10 2 8 14
Golgi 0 0 1 1 0 0 0 0 0 0 0 0 0 0 0 0 2 5 1 0 6 2 3 2
Mitochondria 30 71 15 16 17 66 133 105 104 169 154 201 1 1 0 17 19 8 1 2 2 2 2 8
Nucleus 395 436 387 195 184 334 371 378 382 415 346 358 386 533 459 197 234 228 368 405 356 365 427 433
Nuclear Periphery 1 0 2 1 1 4 4 4 1 11 4 6 3 1 5 1 1 1 0 0 1 0 0 1
Nucleolus 157 86 81 40 44 143 178 274 229 302 311 297 34 37 38 9 6 8 65 56 75 42 64 110
Peroxisomes 1 1 0 0 0 0 0 0 0 1 0 0 0 1 0 0 0 0 0 0 1 0 0 0
SpindlePole 1 3 4 1 0 1 0 2 2 5 3 0 6 13 11 7 4 6 1 1 2 6 7 14
Vac/Vac Membrane 1 6 3 1 6 7 7 3 4 7 5 4 0 6 6 16 18 24 10 12 9 19 23 48
Unique Cell Count 448 486 441 236 227 452 540 600 570 662 593 639 446 660 588 273 291 301 460 491 479 452 551 648
Labelled Cell Count 591 611 507 273 270 585 733 806 778 957 871 908 479 705 621 306 329 337 460 491 479 452 551 648


Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 6.4 6.3 7.1 6.4 6.5 5.9 5.7 5.7 5.7 5.5 5.3 4.8 8.3 8.1 7.6 6.9 6.9 7.2 8.4 8.3 9.0
Std Deviation (1e-4) 1.1 1.5 1.8 2.1 2.2 2.0 2.0 2.1 1.9 1.9 2.2 1.9 2.0 1.9 1.9 1.6 1.7 2.0 1.8 1.7 1.7
Intensity Change (Log2) – – – -0.14 -0.11 -0.27 -0.3 -0.31 -0.31 -0.37 -0.42 -0.55 0.23 0.19 0.1 -0.03 -0.03 0.03 0.25 0.23 0.35


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 3.3 3.2 4.9 4.2 4.7 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 2.5 2.1 1.7 1.2 1.1 0.1 -0.2 -0.7 0 4.8 7.4 7.1 6.4 4.0 6.0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 2.0 2.3 5.8 9.2 7.0 7.3 9.7 9.7 0 -3.6 -4.4 -4.5 1.8 2.0 -0.6
Nucleus -1.8 -2.3 -5.3 -7.1 -8.9 -7.7 -9.2 -10.3 -11.1 -0.5 -3.1 -4.0 -5.2 -2.7 -4.3
Nuclear Periphery 0 0 0 0 0 0 1.8 0 0 0 0 0 0 0 0
Nucleolus -0.5 0.3 4.6 5.2 9.2 7.5 9.3 11.2 0 -4.8 -6.7 -5.9 -5.9 -6.7 -6.5
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 1.4 1.3 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 4.2 4.4 5.2

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 6.9311 6.0473 6.3228 5.6639 6.2283 6.236 4.7851 3.4938 3.9783 3.4536 3.4299 3.6794 4.6163 3.4045 3.6794 3.6926 3.35 3.2276
Actin 0.0001 0.0001 0 0 0 0 0.0116 0 0.0008 0.0002 0.0001 0.0096 0 0 0 0 0 0
Bud 0 0.0001 0 0 0 0 0.0002 0 0.0001 0.0007 0 0.0001 0 0 0 0 0 0
Bud Neck 0.0001 0.0007 0 0.0001 0.0005 0.0006 0.0005 0 0.0002 0.0002 0.0003 0.0008 0 0 0 0 0.0002 0.0003
Bud Periphery 0 0.0004 0 0 0.0001 0 0.0003 0 0.0004 0.0022 0.0001 0.0002 0 0 0 0 0 0
Bud Site 0.0001 0.0005 0 0 0.0001 0.0001 0.001 0 0.0007 0.0019 0.0002 0.0004 0 0 0 0 0 0
Cell Periphery 0 0.0002 0 0 0 0 0.0001 0 0.0001 0.0015 0 0 0 0 0 0 0 0
Cytoplasm 0.0014 0.0003 0 0 0 0.0001 0.0093 0 0.0031 0.0001 0 0.0001 0 0 0 0 0 0
Cytoplasmic Foci 0.0003 0 0 0 0 0 0.0042 0 0.001 0.0001 0.002 0.0011 0 0 0 0 0 0
Eisosomes 0 0 0 0 0 0 0.0002 0 0 0 0 0.0002 0 0 0 0 0 0
Endoplasmic Reticulum 0.0002 0.0002 0 0 0 0 0.004 0 0.0008 0.0001 0 0.0005 0 0 0 0 0 0
Endosome 0.0003 0.0006 0 0 0 0.0001 0.0206 0 0.0016 0.0002 0.0014 0.0029 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0.0052 0 0.0004 0 0.0012 0.0027 0 0 0 0 0 0
Lipid Particles 0.0001 0.0003 0 0 0 0 0.0027 0 0.0014 0.0001 0.0179 0.0013 0 0 0 0 0 0
Mitochondria 0.0002 0.0038 0 0.0001 0.0004 0 0.0026 0 0.0022 0.0059 0.0025 0.0013 0 0 0 0 0.0002 0
None 0.0003 0.0001 0 0 0 0.0001 0.0026 0 0.0003 0.0001 0 0.0002 0 0 0 0 0 0
Nuclear Periphery 0.0008 0.0041 0.0002 0.0003 0.0008 0.0003 0.0126 0.0002 0.0035 0.0003 0.001 0.0036 0.0001 0.0003 0.0002 0.0001 0.0016 0
Nucleolus 0.0419 0.0353 0.0229 0.0497 0.1904 0.1058 0.0411 0.0322 0.0214 0.0177 0.1435 0.104 0.036 0.0285 0.0158 0.021 0.1685 0.1114
Nucleus 0.9486 0.9369 0.9761 0.9491 0.7807 0.8874 0.8714 0.9664 0.955 0.9638 0.8234 0.8673 0.9636 0.9651 0.9828 0.9787 0.8218 0.8878
Peroxisomes 0 0.0001 0 0 0 0 0.0007 0 0.0048 0.0002 0.0048 0.0016 0 0 0 0 0 0
Punctate Nuclear 0.0053 0.0089 0.0007 0.0005 0.0265 0.0052 0.007 0.0011 0.001 0.0002 0.001 0.0016 0.0002 0.006 0.0011 0.0001 0.0075 0.0004
Vacuole 0.0001 0.0045 0 0.0001 0.0001 0.0001 0.0013 0 0.0006 0.0035 0.0003 0.0002 0 0 0 0 0 0
Vacuole Periphery 0.0001 0.0026 0 0 0.0001 0 0.0009 0 0.0004 0.0011 0.0002 0.0003 0 0 0 0 0 0

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 14.9339 11.4429 15.8501 15.3335 14.3836 9.3818 16.005 13.5354 19.9733 16.1968
Translational Efficiency 1.1257 1.3649 0.8552 1.0774 1.0752 1.8574 1.0482 1.244 0.7909 1.1474

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
67 2284 1018 1570 1717 1667 504 275 1784 3951 1522 1845

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 925.75 972.61 1024.92 1157.40 795.35 907.21 1135.24 1126.50 800.25 945.02 1061.45 1152.79
Standard Deviation 201.98 143.37 140.44 186.40 116.66 145.48 148.86 214.48 123.47 147.83 152.40 191.17
Intensity Change Log 2 — 0.071239 0.146817 0.322193 — 0.189847 0.513336 0.502186 — 0.127264 0.327807 0.408169

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.003489 0.000334 0.000706 0.001009 0.000395 0.001205 0.001009 0.001781 0.000512 0.000702 0.000806 0.001124
Bud Neck 0.004422 0.009253 0.024009 0.024933 0.005641 0.014599 0.017165 0.015115 0.005595 0.011508 0.021743 0.023469
Bud Site 0.005480 0.000768 0.001130 0.001824 0.000540 0.001826 0.001280 0.001701 0.000726 0.001214 0.001180 0.001806
Cell Periphery 0.000566 0.000036 0.000027 0.000117 0.000056 0.000108 0.000036 0.000130 0.000075 0.000066 0.000030 0.000119
Cytoplasm 0.019755 0.000979 0.000050 0.000687 0.001044 0.001572 0.000113 0.000789 0.001746 0.001230 0.000071 0.000702
Cytoplasmic Foci 0.013846 0.000298 0.000008 0.000172 0.000573 0.000657 0.000031 0.002856 0.001071 0.000450 0.000015 0.000572
Eisosomes 0.000115 0.000042 0.000061 0.000042 0.000030 0.000050 0.000067 0.000040 0.000033 0.000045 0.000063 0.000042
Endoplasmic Reticulum 0.009940 0.001517 0.001435 0.002059 0.002186 0.003377 0.002204 0.002894 0.002477 0.002302 0.001690 0.002184
Endosome 0.004456 0.000384 0.000052 0.000618 0.000165 0.001123 0.000069 0.003274 0.000326 0.000695 0.000058 0.001014
Golgi 0.006498 0.000250 0.000116 0.001783 0.000139 0.000336 0.000127 0.004053 0.000378 0.000287 0.000120 0.002121
Lipid Particles 0.012280 0.000093 0.000015 0.000055 0.000220 0.000340 0.000036 0.000182 0.000673 0.000197 0.000022 0.000074
Mitochondria 0.027295 0.000712 0.001193 0.003569 0.001252 0.002268 0.001277 0.002386 0.002230 0.001369 0.001221 0.003392
Mitotic Spindle 0.007229 0.001155 0.000429 0.006509 0.000933 0.008658 0.003538 0.015860 0.001170 0.004321 0.001459 0.007903
None 0.013956 0.001468 0.000753 0.001623 0.001921 0.002546 0.001732 0.002360 0.002373 0.001923 0.001077 0.001733
Nuclear Periphery 0.000749 0.000153 0.000078 0.001637 0.000137 0.000962 0.000102 0.000474 0.000160 0.000494 0.000086 0.001463
Nuclear Periphery Foci 0.006344 0.000350 0.000024 0.000517 0.000278 0.000848 0.000047 0.000456 0.000506 0.000560 0.000031 0.000508
Nucleolus 0.085702 0.096964 0.102075 0.052987 0.083165 0.089849 0.079067 0.044219 0.083260 0.093962 0.094456 0.051680
Nucleus 0.747418* 0.884200* 0.866642* 0.893097* 0.899998* 0.866568* 0.890037* 0.895783* 0.894268* 0.876761* 0.874389* 0.893498*
Peroxisomes 0.011300 0.000403 0.000880 0.000959 0.001037 0.001294 0.001441 0.001057 0.001422 0.000779 0.001065 0.000973
Vacuole 0.010485 0.000294 0.000156 0.002624 0.000141 0.001089 0.000456 0.003047 0.000529 0.000630 0.000255 0.002687
Vacuole Periphery 0.008674 0.000346 0.000162 0.003178 0.000150 0.000722 0.000166 0.001545 0.000470 0.000505 0.000164 0.002935

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin 2.82 2.44 2.03 -19.32 -8.67 -2.14 -9.90 -4.42 -1.48 -2.09 -1.08 -5.54 -8.90 -3.65 -5.84
Bud Neck -5.35 -15.22 -16.99 -15.14 -2.19 -10.78 -11.64 -7.89 -3.24 -1.11 -11.22 -19.63 -19.86 -14.10 -3.37
Bud Site 2.33 2.10 1.42 -6.39 -5.11 -4.10 -6.98 -5.36 -2.11 -3.01 -2.79 -5.02 -7.78 -5.16 -5.50
Cell Periphery 2.45 2.49 2.12 -2.07 -2.34 -2.68 1.28 -1.93 -0.31 -2.50 0.56 3.17 -1.00 -1.36 -2.65
Cytoplasm 1.84 1.94 1.85 0.13 -4.26 -1.02 2.33 -0.11 0.88 -2.36 0.84 3.12 1.38 0.94 -4.71
Cytoplasmic Foci 2.43 2.49 2.46 1.03 -3.14 -0.04 1.87 -0.83 -0.83 -1.29 1.68 2.94 1.68 0.21 -1.81
Eisosomes 5.22 3.76 4.99 -1.76 6.97 -7.24 -13.38 -3.62 0.99 5.27 -6.29 -14.57 -6.56 0.15 9.72
Endoplasmic Reticulum 3.93 3.94 3.60 -4.88 -5.26 -5.90 -1.78 -1.75 -0.23 -1.34 0.97 4.31 0.30 -0.51 -3.38
Endosome 1.98 2.14 1.92 -0.55 -2.82 -2.79 2.29 -1.39 -0.73 -1.45 -1.99 3.07 -1.57 -0.20 -2.67
Golgi 2.83 2.89 2.23 -1.46 -1.64 -2.28 -0.45 -1.52 -1.40 -1.51 0.87 2.64 -1.70 -1.86 -2.09
Lipid Particles 2.01 2.02 2.01 0.48 -5.29 -0.64 1.85 0.19 0.89 -2.36 1.91 2.66 2.39 1.81 -4.62
Mitochondria 2.22 2.17 1.96 -11.34 -9.29 -1.85 -0.37 -2.53 -1.08 -4.59 1.19 1.31 -1.91 -8.72 -9.97
Mitotic Spindle 1.36 1.50 -1.01 -5.56 -5.99 -4.26 -1.48 -3.82 -2.83 -3.37 -3.51 -0.59 -6.64 -4.88 -6.22
None 1.45 1.52 1.39 -1.51 -5.50 -1.77 0.24 -1.73 -0.24 -1.55 0.95 2.87 0.70 -0.46 -3.96
Nuclear Periphery 1.88 2.13 -1.62 -3.48 -3.69 -3.45 0.15 -5.63 0.91 -5.80 -3.00 2.04 -3.74 -2.64 -3.92
Nuclear Periphery Foci 2.80 2.97 2.69 -1.14 -4.31 -2.29 2.30 -1.68 -0.84 -2.03 -0.24 3.85 -1.01 -0.76 -4.48
Nucleolus -0.97 -1.74 1.61 7.42 7.29 -1.84 0.32 1.03 1.79 0.74 -3.46 -3.54 3.93 7.37 6.65
Nucleus -3.65 -2.99 -3.22 2.70 -1.31 6.74 2.46 4.02 1.69 2.79 4.38 5.35 5.10 2.20 0.57
Peroxisomes 3.27 3.11 3.04 -7.36 -1.73 -0.50 -1.45 -1.43 -0.76 -0.19 1.71 0.78 0.53 -2.55 -0.74
Vacuole 2.35 2.38 1.81 -4.23 -4.51 -4.13 -5.24 -2.08 -1.37 -1.67 -0.71 0.94 -4.02 -3.87 -4.56
Vacuole Periphery 2.00 2.05 1.50 -2.79 -3.06 -3.11 -1.46 -1.11 -0.45 -1.08 -0.24 1.86 -2.66 -2.65 -3.22
Standard name
Human Ortholog
Description Component of the histone deacetylase Rpd3L complex; possibly involved in cell fusion and invasive growth; relocalizes to the cytosol in response to hypoxia
Localization
Cell Percentages nucleus (98%)
Cell Cycle Regulation No
Subcompartmental Group nucleus-4

Rxt2

Rxt2


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Rxt2-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available