Standard name | |
---|---|
Human Ortholog | |
Description | GID Complex regulatory subunit; binds GID Complex in response to glucose through interactions with complex member Vid28p; regulates fructose-1,6-bisphosphatase (FBPase) targeting to the vacuole; promotes proteasome-dependent catabolite degradation of FBPase; peripheral membrane protein located at Vid (vacuole import and degradation) vesicles |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 1 | – | 2 | 1 | 3 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 2 |
Bud | 0 | 0 | 0 | 0 | 1 | 5 | 5 | 6 | – | 2 | 9 | 13 | 0 | 0 | 0 | 0 | 0 | 0 | 3 | 0 | 1 | 1 | 3 | 5 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 3 | 0 | 2 | 3 | 3 | 0 | 4 | – | 7 | 14 | 22 | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 309 | 139 | 377 | 320 | 462 | 533 | 393 | 422 | – | 424 | 356 | 545 | 248 | 279 | 304 | 59 | 78 | 60 | 282 | 131 | 340 | 148 | 267 | 210 |
Endoplasmic Reticulum | 3 | 2 | 0 | 2 | 7 | 5 | 11 | 9 | – | 22 | 6 | 19 | 3 | 6 | 1 | 3 | 6 | 1 | 2 | 1 | 2 | 2 | 7 | 11 |
Endosome | 0 | 0 | 0 | 0 | 0 | 3 | 10 | 4 | – | 3 | 3 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 3 | 18 |
Golgi | 0 | 2 | 1 | 0 | 2 | 0 | 0 | 0 | – | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 0 | 2 | 1 | 0 | 0 | 0 | 1 | 15 |
Mitochondria | 0 | 0 | 0 | 1 | 8 | 50 | 94 | 83 | – | 80 | 183 | 190 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3 |
Nucleus | 2 | 1 | 2 | 3 | 7 | 43 | 48 | 60 | – | 65 | 41 | 64 | 1 | 2 | 4 | 0 | 0 | 1 | 26 | 22 | 15 | 15 | 34 | 27 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | – | 3 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 3 | 1 |
Nucleolus | 0 | 8 | 0 | 0 | 0 | 0 | 8 | 6 | – | 2 | 0 | 2 | 1 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 4 |
Vac/Vac Membrane | 30 | 4 | 12 | 49 | 58 | 64 | 307 | 231 | – | 199 | 129 | 161 | 26 | 41 | 57 | 7 | 20 | 7 | 3 | 8 | 3 | 9 | 26 | 34 |
Unique Cell Count | 326 | 150 | 385 | 344 | 517 | 655 | 801 | 753 | 733 | 656 | 917 | 265 | 310 | 342 | 62 | 87 | 65 | 327 | 168 | 374 | 181 | 355 | 342 | |
Labelled Cell Count | 344 | 159 | 392 | 377 | 548 | 706 | 880 | 826 | 809 | 742 | 1022 | 281 | 329 | 368 | 69 | 106 | 71 | 327 | 168 | 374 | 181 | 355 | 342 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 19.5 | 23.2 | 13.6 | 15.2 | 12.3 | 9.0 | 7.9 | 8.1 | – | 8.4 | 8.6 | 9.2 | 21.7 | 19.8 | 19.3 | 17.6 | 20.5 | 19.9 | 20.3 | 20.2 | 20.1 |
Std Deviation (1e-4) | 3.9 | 4.2 | 2.7 | 3.3 | 2.5 | 1.8 | 1.6 | 1.8 | – | 1.9 | 2.0 | 2.4 | 4.7 | 4.1 | 3.8 | 4.3 | 4.8 | 5.6 | 4.1 | 4.7 | 4.8 |
Intensity Change (Log2) | – | – | – | 0.16 | -0.15 | -0.59 | -0.79 | -0.75 | – | -0.71 | -0.66 | -0.57 | 0.67 | 0.54 | 0.5 | 0.37 | 0.59 | 0.55 | 0.57 | 0.57 | 0.56 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | – | 0 | 2.9 | 3.1 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | -3.2 | -5.0 | -7.8 | -16.5 | -14.6 | – | -14.1 | -14.9 | -13.9 | -2.8 | -4.5 | -5.0 | -1.3 | -3.7 | -2.5 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | – | 3.4 | 0 | 2.8 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 5.6 | 7.0 | 6.8 | – | 6.7 | 11.4 | 9.7 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 0 | 0 | 4.6 | 4.4 | 5.2 | – | 5.6 | 4.5 | 4.8 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 5.4 | 4.5 | 4.0 | 12.8 | 10.7 | – | 9.8 | 7.5 | 7.0 | 3.6 | 5.0 | 6.2 | 0 | 6.7 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 13.3662 | 20.2504 | 17.3002 | 16.8234 | 16.8923 | 18.1051 | 20.925 | 26.0164 | 24.0125 | 24.2278 | 21.4661 | 23.3228 | 17.1766 | 22.617 | 20.6613 | 15.8321 | 20.0162 | 19.4779 |
Actin | 0.0053 | 0.0015 | 0.002 | 0.0045 | 0.0041 | 0.002 | 0.0128 | 0.0017 | 0.0014 | 0.0038 | 0.0014 | 0.0047 | 0.0005 | 0.0003 | 0.0005 | 0.0013 | 0.0013 | 0.0015 |
Bud | 0.0014 | 0.002 | 0.0009 | 0.0003 | 0.0006 | 0.0005 | 0.0016 | 0.0021 | 0.0022 | 0.0103 | 0.0009 | 0.0023 | 0.0001 | 0.0002 | 0.0002 | 0.0004 | 0.0001 | 0.0005 |
Bud Neck | 0.0025 | 0.0004 | 0.0009 | 0.0032 | 0.001 | 0.0015 | 0.0008 | 0.0003 | 0.0006 | 0.0009 | 0.0008 | 0.0017 | 0.0004 | 0.0003 | 0.0004 | 0.0008 | 0.0007 | 0.0011 |
Bud Periphery | 0.0011 | 0.0004 | 0.0008 | 0.0005 | 0.0003 | 0.0003 | 0.0007 | 0.0008 | 0.0007 | 0.0048 | 0.0003 | 0.0018 | 0 | 0 | 0 | 0.0002 | 0 | 0.0003 |
Bud Site | 0.0052 | 0.0026 | 0.0029 | 0.0116 | 0.0003 | 0.0002 | 0.0033 | 0.0025 | 0.0029 | 0.0004 | 0.0005 | 0.0002 | 0.0001 | 0.0004 | 0.0002 | 0.0005 | 0 | 0.0001 |
Cell Periphery | 0.0003 | 0 | 0.0001 | 0.0003 | 0 | 0 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 0.6502 | 0.8294 | 0.7273 | 0.6395 | 0.7303 | 0.7267 | 0.7898 | 0.8868 | 0.8335 | 0.8861 | 0.8643 | 0.7979 | 0.8386 | 0.8885 | 0.8192 | 0.7494 | 0.8523 | 0.7569 |
Cytoplasmic Foci | 0.0455 | 0.0056 | 0.0106 | 0.0231 | 0.0146 | 0.0097 | 0.0075 | 0.0009 | 0.002 | 0.0009 | 0.0027 | 0.0031 | 0.001 | 0.001 | 0.0008 | 0.0074 | 0.0016 | 0.0009 |
Eisosomes | 0 | 0 | 0 | 0.0002 | 0 | 0 | 0.0001 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endoplasmic Reticulum | 0.008 | 0.001 | 0.0022 | 0.0011 | 0.0053 | 0.0023 | 0.004 | 0.0008 | 0.001 | 0.0029 | 0.0023 | 0.0028 | 0.0022 | 0.0005 | 0.0011 | 0.0025 | 0.0011 | 0.0018 |
Endosome | 0.031 | 0.0025 | 0.0216 | 0.0187 | 0.0198 | 0.0066 | 0.0064 | 0.0002 | 0.0006 | 0.0009 | 0.0008 | 0.0014 | 0.0005 | 0.0001 | 0.0004 | 0.0016 | 0.0004 | 0.0005 |
Golgi | 0.0063 | 0.0012 | 0.0051 | 0.0058 | 0.0211 | 0.0022 | 0.0014 | 0 | 0.0001 | 0.0001 | 0.0001 | 0.0002 | 0 | 0 | 0 | 0.0002 | 0 | 0 |
Lipid Particles | 0.0152 | 0.0001 | 0.0007 | 0.0068 | 0.0052 | 0.0012 | 0.0058 | 0 | 0 | 0 | 0.0001 | 0.0001 | 0 | 0 | 0 | 0.0003 | 0 | 0 |
Mitochondria | 0.006 | 0.0005 | 0.0049 | 0.0037 | 0.0032 | 0.0005 | 0.0062 | 0.0002 | 0.0008 | 0.0004 | 0.0002 | 0.0005 | 0.0002 | 0.0001 | 0.0001 | 0.0002 | 0.0001 | 0.0001 |
None | 0.0051 | 0.0009 | 0.0019 | 0.002 | 0.0014 | 0.0008 | 0.0044 | 0.0011 | 0.002 | 0.0018 | 0.0014 | 0.0025 | 0.001 | 0.0006 | 0.0005 | 0.0021 | 0.0006 | 0.0006 |
Nuclear Periphery | 0.0286 | 0.0084 | 0.0135 | 0.0106 | 0.0184 | 0.0142 | 0.0133 | 0.0063 | 0.0088 | 0.0097 | 0.0125 | 0.0122 | 0.0135 | 0.0067 | 0.0112 | 0.0139 | 0.0098 | 0.0142 |
Nucleolus | 0.0025 | 0.0001 | 0.0006 | 0.0006 | 0.0001 | 0.0001 | 0.0006 | 0.0001 | 0.0003 | 0.0001 | 0.0002 | 0.0003 | 0 | 0 | 0.0001 | 0.0002 | 0 | 0.0001 |
Nucleus | 0.1454 | 0.1402 | 0.194 | 0.2596 | 0.1609 | 0.226 | 0.1242 | 0.0944 | 0.1382 | 0.0736 | 0.0983 | 0.1625 | 0.1398 | 0.0994 | 0.1631 | 0.2005 | 0.129 | 0.2185 |
Peroxisomes | 0.0042 | 0.0004 | 0.0025 | 0.0033 | 0.0011 | 0.001 | 0.0072 | 0.0001 | 0.0002 | 0.0001 | 0.0001 | 0.0002 | 0 | 0 | 0 | 0.0003 | 0.0001 | 0 |
Punctate Nuclear | 0.0192 | 0.0019 | 0.0024 | 0.0018 | 0.0098 | 0.0028 | 0.0064 | 0.0012 | 0.0035 | 0.0022 | 0.0121 | 0.0039 | 0.0012 | 0.0013 | 0.0017 | 0.0172 | 0.0023 | 0.0019 |
Vacuole | 0.0135 | 0.0006 | 0.004 | 0.0021 | 0.0017 | 0.0012 | 0.0028 | 0.0004 | 0.001 | 0.0008 | 0.0009 | 0.0014 | 0.0006 | 0.0002 | 0.0004 | 0.0008 | 0.0004 | 0.0007 |
Vacuole Periphery | 0.0034 | 0.0001 | 0.0011 | 0.0006 | 0.0008 | 0.0003 | 0.0006 | 0.0001 | 0.0003 | 0.0002 | 0.0002 | 0.0005 | 0.0001 | 0.0001 | 0.0001 | 0.0002 | 0.0001 | 0.0001 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 25.3307 | 28.9844 | 20.8356 | 23.3491 | 21.0191 | 28.3547 | 41.6696 | 37.3959 | 38.3954 | 35.6069 |
Translational Efficiency | 1.0218 | 0.8307 | 0.9068 | 0.6325 | 0.6685 | 1.0712 | 0.7833 | 0.8928 | 0.8234 | 0.8147 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
377 | 1945 | 1173 | 1541 | 2028 | 1484 | 1040 | 1576 | 2405 | 3429 | 2213 | 3117 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 887.84 | 1326.17 | 1542.80 | 1438.80 | 1038.00 | 1199.07 | 1584.23 | 1477.87 | 1014.46 | 1271.16 | 1562.27 | 1458.55 |
Standard Deviation | 176.49 | 451.45 | 331.94 | 244.47 | 294.07 | 409.29 | 263.99 | 247.79 | 284.22 | 438.26 | 302.62 | 246.93 |
Intensity Change Log 2 | — | 0.578894 | 0.797179 | 0.696494 | — | 0.208109 | 0.609975 | 0.509713 | — | 0.390933 | 0.699305 | 0.598834 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.000313 | 0.000274 | 0.000353 | 0.001115 | 0.000188 | 0.000318 | 0.000378 | 0.000796 | 0.000207 | 0.000293 | 0.000365 | 0.000954 |
Bud Neck | 0.000858 | 0.002813 | 0.002772 | 0.010991 | 0.002576 | 0.003505 | 0.002921 | 0.010186 | 0.002306 | 0.003112 | 0.002842 | 0.010584 |
Bud Site | 0.001832 | 0.003083 | 0.001064 | 0.025308 | 0.003393 | 0.006876 | 0.003084 | 0.022622 | 0.003148 | 0.004724 | 0.002013 | 0.023950 |
Cell Periphery | 0.000224 | 0.000157 | 0.000122 | 0.000471 | 0.000134 | 0.000181 | 0.000121 | 0.000149 | 0.000148 | 0.000167 | 0.000121 | 0.000308 |
Cytoplasm | 0.471024* | 0.436209* | 0.126873* | 0.269741* | 0.578639* | 0.594240* | 0.178925* | 0.334606* | 0.561770* | 0.504601* | 0.151335* | 0.302538* |
Cytoplasmic Foci | 0.029108 | 0.005327 | 0.000639 | 0.008062 | 0.012215 | 0.013848 | 0.001743 | 0.012214 | 0.014863 | 0.009015 | 0.001158 | 0.010161 |
Eisosomes | 0.000031 | 0.000007 | 0.000005 | 0.000017 | 0.000009 | 0.000011 | 0.000008 | 0.000015 | 0.000012 | 0.000009 | 0.000006 | 0.000016 |
Endoplasmic Reticulum | 0.003250 | 0.001491 | 0.001180 | 0.001529 | 0.001524 | 0.001377 | 0.001265 | 0.001598 | 0.001795 | 0.001441 | 0.001220 | 0.001564 |
Endosome | 0.001803 | 0.000609 | 0.000203 | 0.003734 | 0.000521 | 0.000941 | 0.000854 | 0.012943 | 0.000722 | 0.000753 | 0.000509 | 0.008390 |
Golgi | 0.000244 | 0.000168 | 0.000085 | 0.005473 | 0.000195 | 0.000477 | 0.000119 | 0.008471 | 0.000203 | 0.000302 | 0.000101 | 0.006989 |
Lipid Particles | 0.002573 | 0.000314 | 0.000189 | 0.000820 | 0.000422 | 0.001608 | 0.000329 | 0.001169 | 0.000759 | 0.000874 | 0.000255 | 0.000997 |
Mitochondria | 0.000266 | 0.000553 | 0.000781 | 0.002774 | 0.000573 | 0.000542 | 0.000886 | 0.005667 | 0.000525 | 0.000548 | 0.000830 | 0.004237 |
Mitotic Spindle | 0.000092 | 0.003850 | 0.002863 | 0.109204* | 0.002350 | 0.007199 | 0.005269 | 0.179009* | 0.001996 | 0.005299 | 0.003994 | 0.144499* |
None | 0.009394 | 0.021250 | 0.006230 | 0.012660 | 0.015808 | 0.019637 | 0.008719 | 0.012699 | 0.014803 | 0.020552 | 0.007400 | 0.012680 |
Nuclear Periphery | 0.000402 | 0.000545 | 0.000685 | 0.001733 | 0.000373 | 0.000339 | 0.001006 | 0.003566 | 0.000378 | 0.000456 | 0.000836 | 0.002660 |
Nuclear Periphery Foci | 0.003865 | 0.001237 | 0.000538 | 0.002220 | 0.001334 | 0.001810 | 0.000536 | 0.006145 | 0.001731 | 0.001485 | 0.000537 | 0.004204 |
Nucleolus | 0.000825 | 0.000583 | 0.000155 | 0.000701 | 0.000375 | 0.000634 | 0.000225 | 0.001350 | 0.000446 | 0.000605 | 0.000188 | 0.001029 |
Nucleus | 0.456609* | 0.515286* | 0.849316* | 0.509828* | 0.368728* | 0.332044* | 0.777717* | 0.332988* | 0.382504* | 0.435983* | 0.815668* | 0.420415* |
Peroxisomes | 0.000890 | 0.000113 | 0.000030 | 0.000673 | 0.000250 | 0.000424 | 0.000410 | 0.002087 | 0.000351 | 0.000248 | 0.000209 | 0.001388 |
Vacuole | 0.016216 | 0.005953 | 0.005865 | 0.029541 | 0.010238 | 0.013437 | 0.015428 | 0.048471 | 0.011175 | 0.009192 | 0.010359 | 0.039112 |
Vacuole Periphery | 0.000184 | 0.000178 | 0.000053 | 0.003401 | 0.000153 | 0.000553 | 0.000058 | 0.003248 | 0.000158 | 0.000340 | 0.000055 | 0.003324 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | 0.16 | -4.34 | -5.27 | -5.59 | -3.18 | -4.88 | -8.87 | -8.67 | -5.29 | -1.34 | -5.29 | -11.39 | -9.51 | -7.56 | -3.43 |
Bud Neck | -7.46 | -6.33 | -13.38 | -11.16 | -9.69 | -1.91 | -4.46 | -10.54 | -8.42 | -3.45 | -3.02 | -5.55 | -15.71 | -14.13 | -9.50 |
Bud Site | -2.24 | 0.68 | -8.97 | -7.96 | -9.31 | -3.18 | -0.92 | -7.60 | -4.62 | -6.60 | -2.49 | 0.56 | -10.79 | -9.03 | -11.22 |
Cell Periphery | 2.89 | 1.32 | -1.16 | -2.92 | -2.04 | -3.84 | -3.73 | -2.05 | 2.62 | 2.59 | -2.43 | -3.64 | -3.26 | -2.07 | -1.07 |
Cytoplasm | 2.99 | 23.23 | 17.06 | 29.38 | -14.77 | 0.02 | 58.64 | 38.78 | 35.46 | -17.22 | 10.06 | 70.02 | 49.68 | 43.06 | -22.80 |
Cytoplasmic Foci | 6.90 | 8.22 | 6.80 | -0.24 | -6.60 | -1.35 | 13.15 | 5.53 | 5.96 | -6.63 | 5.91 | 16.08 | 9.21 | 4.11 | -9.41 |
Eisosomes | 8.32 | 7.84 | 1.67 | -13.66 | -12.39 | -3.11 | -5.87 | -15.07 | -9.92 | -8.21 | 4.25 | 1.74 | -12.13 | -17.55 | -15.09 |
Endoplasmic Reticulum | 4.72 | 4.62 | 3.54 | -3.34 | -2.99 | 1.21 | -2.20 | -1.24 | -2.47 | 1.26 | 2.89 | 1.04 | -0.13 | -4.00 | -1.54 |
Endosome | 5.09 | 6.27 | 0.80 | -4.39 | -5.66 | -3.26 | -1.04 | -4.35 | -3.47 | -3.72 | 0.03 | 2.24 | -5.11 | -5.08 | -5.75 |
Golgi | 0.94 | 1.51 | -1.57 | -1.77 | -1.87 | -1.92 | 1.04 | -2.30 | -1.69 | -2.41 | -1.35 | 1.62 | -2.82 | -2.52 | -3.04 |
Lipid Particles | 1.86 | 1.81 | 0.19 | -14.08 | -13.43 | -1.67 | -3.54 | -11.83 | -0.88 | -10.12 | -0.22 | 1.06 | -6.60 | -4.73 | -16.87 |
Mitochondria | -5.46 | -10.97 | -16.12 | -13.18 | -8.43 | 0.30 | -5.34 | -4.62 | -4.99 | -2.58 | -0.23 | -5.75 | -8.41 | -9.58 | -5.81 |
Mitotic Spindle | -4.38 | -3.06 | -15.67 | -14.77 | -14.71 | -2.81 | -3.62 | -14.56 | -13.36 | -12.68 | -3.22 | -3.57 | -21.03 | -20.00 | -19.45 |
None | -5.86 | 2.24 | -3.05 | 4.76 | -12.04 | -1.77 | 6.17 | 1.34 | 3.20 | -7.08 | -4.08 | 7.96 | 0.37 | 5.76 | -13.64 |
Nuclear Periphery | -4.58 | -7.89 | -24.82 | -23.46 | -17.64 | 0.36 | -18.41 | -12.61 | -12.64 | -6.44 | -4.47 | -17.58 | -23.58 | -22.76 | -15.14 |
Nuclear Periphery Foci | 3.95 | 4.83 | 0.54 | -4.79 | -6.11 | -3.10 | 2.46 | -3.19 | -1.57 | -4.15 | 0.63 | 5.30 | -4.11 | -4.59 | -7.24 |
Nucleolus | 2.35 | 6.50 | -3.81 | -4.96 | -6.53 | -1.85 | -1.59 | -4.77 | -2.56 | -1.16 | -2.10 | -0.60 | -7.03 | -5.43 | -4.65 |
Nucleus | -4.37 | -24.39 | -3.37 | 1.47 | 36.84 | 2.93 | -46.20 | -7.19 | -9.33 | 34.04 | -9.13 | -63.60 | -12.02 | -3.60 | 50.12 |
Peroxisomes | 1.38 | 1.48 | 0.68 | -4.12 | -4.77 | -0.99 | 0.35 | -2.81 | -1.73 | -3.02 | 0.78 | 1.68 | -1.84 | -3.75 | -5.01 |
Vacuole | 4.18 | 1.51 | -20.13 | -23.91 | -22.09 | -4.64 | -9.17 | -25.54 | -23.29 | -17.19 | -0.23 | -7.39 | -33.49 | -33.63 | -27.61 |
Vacuole Periphery | 0.39 | 5.68 | -1.88 | -1.94 | -2.42 | -1.00 | 2.23 | -2.37 | -0.13 | -2.68 | -1.03 | 4.20 | -3.03 | -1.28 | -3.56 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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