Standard name | |
---|---|
Human Ortholog | |
Description | Synthase subunit of trehalose-6-P synthase/phosphatase complex; synthesizes the storage carbohydrate trehalose, which is critically important for survival of long-term desiccation; also found in a monomeric form; expression is induced by the stress response and repressed by the Ras-cAMP pathway; protein abundance increases in response to DNA replication stress and in response to prolonged exposure to boric acid |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 2 | 4 | 5 | 9 | 6 | 11 | 9 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 1 | 0 | 3 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 1 | 1 | 0 | 2 | 1 | 0 | 0 | 1 | 1 | 0 | 2 | 0 | 0 | 1 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 2 | 9 | 7 | 3 | 8 | 11 | 7 | 13 | 15 | 14 | 13 | 18 | 3 | 2 | 6 | 8 | 14 | 12 | 0 | 0 | 1 | 0 | 0 | 0 |
Cytoplasm | 232 | 254 | 260 | 126 | 197 | 246 | 220 | 234 | 202 | 164 | 125 | 169 | 197 | 284 | 291 | 230 | 260 | 269 | 230 | 247 | 241 | 153 | 217 | 144 |
Endoplasmic Reticulum | 2 | 4 | 4 | 0 | 10 | 7 | 36 | 53 | 31 | 26 | 20 | 31 | 2 | 3 | 5 | 8 | 12 | 7 | 1 | 2 | 13 | 3 | 7 | 4 |
Endosome | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 1 |
Golgi | 0 | 0 | 0 | 0 | 1 | 1 | 1 | 3 | 6 | 4 | 2 | 7 | 0 | 0 | 0 | 3 | 5 | 0 | 0 | 0 | 3 | 0 | 1 | 1 |
Mitochondria | 1 | 0 | 0 | 1 | 1 | 3 | 1 | 4 | 8 | 3 | 13 | 18 | 0 | 1 | 0 | 4 | 2 | 0 | 0 | 0 | 1 | 0 | 0 | 0 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 1 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 0 | 1 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 1 | 1 | 2 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vac/Vac Membrane | 0 | 0 | 1 | 0 | 4 | 0 | 2 | 10 | 3 | 9 | 4 | 6 | 1 | 0 | 0 | 9 | 15 | 3 | 2 | 1 | 1 | 1 | 1 | 0 |
Unique Cell Count | 234 | 259 | 265 | 128 | 214 | 259 | 268 | 305 | 255 | 216 | 174 | 238 | 199 | 287 | 295 | 246 | 281 | 279 | 242 | 258 | 273 | 166 | 238 | 156 |
Labelled Cell Count | 237 | 267 | 273 | 130 | 221 | 270 | 276 | 324 | 276 | 227 | 191 | 261 | 203 | 290 | 304 | 263 | 310 | 293 | 242 | 258 | 273 | 166 | 238 | 156 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 20.9 | 36.4 | 30.9 | 31.0 | 36.7 | 37.4 | 39.5 | 38.7 | 41.0 | 42.8 | 40.4 | 44.8 | 41.8 | 59.6 | 64.8 | 35.6 | 41.7 | 45.1 | 22.4 | 24.2 | 28.3 |
Std Deviation (1e-4) | 4.5 | 8.4 | 11.0 | 11.4 | 11.3 | 9.7 | 12.2 | 12.2 | 13.6 | 16.9 | 16.6 | 19.6 | 13.1 | 21.9 | 27.8 | 13.4 | 13.0 | 13.5 | 6.6 | 5.7 | 8.1 |
Intensity Change (Log2) | – | – | – | 0.0 | 0.25 | 0.27 | 0.35 | 0.32 | 0.41 | 0.47 | 0.39 | 0.54 | 0.44 | 0.95 | 1.07 | 0.2 | 0.43 | 0.54 | -0.47 | -0.35 | -0.13 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0.7 | 1.0 | 0 | 1.0 | 1.8 | 2.1 | 2.4 | 2.5 | 0 | 0 | -0.5 | 0.4 | 1.4 | 1.1 |
Cytoplasm | 0.2 | -3.1 | -2.0 | -6.2 | -7.5 | -6.8 | -7.5 | -8.2 | -8.6 | 0.8 | 0.8 | 0.5 | -2.6 | -3.1 | -1.2 |
Endoplasmic Reticulum | 0 | 2.0 | 1.0 | 5.2 | 6.3 | 4.8 | 4.7 | 4.5 | 5.1 | 0 | 0 | 0 | 1.3 | 1.9 | 0.8 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 4.5 | 4.6 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | 0 | 0 | 0 | 2.5 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3.4 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 33.8124 | 44.4769 | 39.5281 | 36.5198 | 37.4122 | 41.5602 | 32.2716 | 43.6869 | 39.4567 | 40.2931 | 21.3442 | 37.6519 | 48.0105 | 51.2604 | 46.3155 | 40.9043 | 38.1426 | 48.1277 |
Actin | 0.027 | 0.0001 | 0.0064 | 0.0107 | 0.0054 | 0.0011 | 0.0127 | 0.0001 | 0.0006 | 0 | 0.0206 | 0.0091 | 0.0075 | 0.0038 | 0.0007 | 0.0038 | 0.0118 | 0.0003 |
Bud | 0.0015 | 0.0001 | 0.0003 | 0.0011 | 0.0021 | 0.0003 | 0.0011 | 0 | 0.0015 | 0.0001 | 0.0004 | 0.0012 | 0.0001 | 0.0002 | 0.0004 | 0.0012 | 0.0053 | 0.0001 |
Bud Neck | 0.0023 | 0.0002 | 0.0004 | 0.0007 | 0.0008 | 0.0015 | 0.0009 | 0.0001 | 0.0003 | 0.0002 | 0.0006 | 0.0008 | 0.0003 | 0.0001 | 0.0003 | 0.0005 | 0.0005 | 0.0004 |
Bud Periphery | 0.0017 | 0.0001 | 0.0004 | 0.0011 | 0.0019 | 0.0002 | 0.0022 | 0 | 0.0004 | 0 | 0.0004 | 0.001 | 0.0001 | 0.0002 | 0.0004 | 0.0011 | 0.0033 | 0 |
Bud Site | 0.0026 | 0.0001 | 0.0006 | 0.0004 | 0.0075 | 0.0001 | 0.0019 | 0 | 0.0011 | 0 | 0.0011 | 0.0009 | 0.0002 | 0.0003 | 0.0003 | 0.0015 | 0.0125 | 0 |
Cell Periphery | 0.0007 | 0.0002 | 0.0008 | 0.0007 | 0.0009 | 0.0003 | 0.0011 | 0 | 0.0004 | 0 | 0.0001 | 0.0001 | 0.0001 | 0 | 0.0004 | 0.0002 | 0.0002 | 0 |
Cytoplasm | 0.7892 | 0.9864 | 0.9043 | 0.9034 | 0.805 | 0.9004 | 0.7904 | 0.9979 | 0.9239 | 0.9919 | 0.5911 | 0.9312 | 0.9332 | 0.9647 | 0.9289 | 0.8985 | 0.8619 | 0.987 |
Cytoplasmic Foci | 0.0299 | 0.0028 | 0.0137 | 0.0078 | 0.0244 | 0.0087 | 0.0146 | 0.0004 | 0.0183 | 0.0015 | 0.079 | 0.0069 | 0.0039 | 0.0036 | 0.0078 | 0.0155 | 0.0262 | 0.0016 |
Eisosomes | 0.0006 | 0 | 0.0002 | 0.0003 | 0.0008 | 0 | 0.0003 | 0 | 0 | 0 | 0.0001 | 0 | 0 | 0.0001 | 0.0002 | 0.0001 | 0.0001 | 0 |
Endoplasmic Reticulum | 0.009 | 0.0017 | 0.0057 | 0.0086 | 0.0041 | 0.0082 | 0.0262 | 0.0002 | 0.0023 | 0.0004 | 0.0191 | 0.0016 | 0.006 | 0.0004 | 0.0082 | 0.0073 | 0.0041 | 0.006 |
Endosome | 0.014 | 0.0008 | 0.0073 | 0.0053 | 0.0215 | 0.0155 | 0.0272 | 0.0002 | 0.0124 | 0.0017 | 0.0999 | 0.0058 | 0.0171 | 0.0009 | 0.0065 | 0.0062 | 0.009 | 0.0012 |
Golgi | 0.003 | 0 | 0.0036 | 0.0008 | 0.0073 | 0.0021 | 0.0099 | 0 | 0.0017 | 0 | 0.0129 | 0.0046 | 0.0054 | 0.0018 | 0.0005 | 0.002 | 0.0044 | 0.0001 |
Lipid Particles | 0.0073 | 0.0001 | 0.0073 | 0.0023 | 0.0399 | 0.0026 | 0.004 | 0 | 0.0009 | 0 | 0.039 | 0.0021 | 0.0025 | 0.0096 | 0.0034 | 0.0013 | 0.0065 | 0 |
Mitochondria | 0.0108 | 0 | 0.0082 | 0.0013 | 0.0403 | 0.0184 | 0.0277 | 0 | 0.0198 | 0.0001 | 0.0035 | 0.0038 | 0.0037 | 0.0111 | 0.0082 | 0.049 | 0.0194 | 0.0001 |
None | 0.0106 | 0.0003 | 0.0029 | 0.0104 | 0.0034 | 0.0005 | 0.0112 | 0.0001 | 0.0043 | 0.0003 | 0.0083 | 0.0003 | 0.0008 | 0.0003 | 0.0082 | 0.0019 | 0.0075 | 0.0002 |
Nuclear Periphery | 0.0165 | 0.0011 | 0.0065 | 0.0053 | 0.0034 | 0.0054 | 0.032 | 0.0002 | 0.001 | 0.0008 | 0.0746 | 0.0013 | 0.0049 | 0.0004 | 0.0041 | 0.0011 | 0.0024 | 0.0009 |
Nucleolus | 0.0066 | 0 | 0.0011 | 0.0002 | 0.0056 | 0.004 | 0.0015 | 0 | 0.0003 | 0 | 0.0016 | 0.0211 | 0.0001 | 0 | 0.0024 | 0.0002 | 0.0008 | 0 |
Nucleus | 0.0142 | 0.0014 | 0.007 | 0.0053 | 0.0055 | 0.0046 | 0.0128 | 0.0004 | 0.0016 | 0.0014 | 0.0094 | 0.0037 | 0.0047 | 0.0007 | 0.0038 | 0.0012 | 0.0034 | 0.0012 |
Peroxisomes | 0.0026 | 0 | 0.0015 | 0.0022 | 0.003 | 0.0012 | 0.0008 | 0 | 0.0005 | 0 | 0.0083 | 0.0012 | 0.0008 | 0.0014 | 0.0019 | 0.0028 | 0.0094 | 0 |
Punctate Nuclear | 0.0197 | 0.0001 | 0.0017 | 0.0031 | 0.0008 | 0.0007 | 0.0045 | 0 | 0.0004 | 0 | 0.0199 | 0.0015 | 0.0002 | 0.0001 | 0.0007 | 0.0001 | 0.0062 | 0.0001 |
Vacuole | 0.0223 | 0.004 | 0.0112 | 0.0213 | 0.0101 | 0.0163 | 0.0135 | 0.0003 | 0.0065 | 0.0012 | 0.0054 | 0.0012 | 0.007 | 0.0003 | 0.0089 | 0.0029 | 0.0037 | 0.0005 |
Vacuole Periphery | 0.0081 | 0.0005 | 0.009 | 0.0077 | 0.0063 | 0.008 | 0.0035 | 0.0001 | 0.0016 | 0.0003 | 0.0046 | 0.0005 | 0.0013 | 0.0002 | 0.0036 | 0.0013 | 0.0013 | 0.0002 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 66.7382 | 45.7392 | 80.1782 | 140.3026 | 85.5675 | 131.1791 | 86.9642 | 128.4731 | 197.0533 | 106.8383 |
Translational Efficiency | 2.2837 | 2.2216 | 1.9841 | 2.104 | 2.2195 | 1.9921 | 1.9793 | 1.9436 | 1.7251 | 1.7764 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✔ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
140 | 1203 | 587 | 1519 | 2495 | 1967 | 2730 | 1133 | 2635 | 3170 | 3317 | 2652 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 1791.10 | 1613.53 | 5228.98 | 4132.07 | 1687.55 | 1690.05 | 4163.73 | 4139.46 | 1693.05 | 1661.01 | 4352.24 | 4135.23 |
Standard Deviation | 415.38 | 371.12 | 1174.31 | 1209.88 | 333.01 | 343.70 | 992.84 | 1187.87 | 338.69 | 356.30 | 1104.81 | 1200.53 |
Intensity Change Log 2 | — | -0.150625 | 1.545684 | 1.206019 | — | 0.002136 | 1.302946 | 1.294512 | — | -0.074497 | 1.433014 | 1.249627 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.000279 | 0.000274 | 0.000136 | 0.000278 | 0.000042 | 0.000164 | 0.000025 | 0.000275 | 0.000055 | 0.000206 | 0.000045 | 0.000276 |
Bud Neck | 0.000504 | 0.000534 | 0.002845 | 0.001454 | 0.000201 | 0.000393 | 0.000570 | 0.002231 | 0.000217 | 0.000446 | 0.000973 | 0.001786 |
Bud Site | 0.020984 | 0.009767 | 0.004348 | 0.010784 | 0.004882 | 0.009165 | 0.001719 | 0.010258 | 0.005738 | 0.009394 | 0.002184 | 0.010559 |
Cell Periphery | 0.003035 | 0.000342 | 0.015180 | 0.006339 | 0.000155 | 0.000178 | 0.000637 | 0.013264 | 0.000308 | 0.000240 | 0.003211 | 0.009298 |
Cytoplasm | 0.711699* | 0.925067* | 0.730407* | 0.839762* | 0.946581* | 0.919945* | 0.903733* | 0.761055* | 0.934101* | 0.921889* | 0.873060* | 0.806136* |
Cytoplasmic Foci | 0.083105 | 0.020160 | 0.027001 | 0.006378 | 0.021874 | 0.027238 | 0.004234 | 0.008723 | 0.025127 | 0.024552 | 0.008263 | 0.007380 |
Eisosomes | 0.000002 | 0.000002 | 0.000003 | 0.000003 | 0.000001 | 0.000001 | 0.000000 | 0.000004 | 0.000001 | 0.000001 | 0.000001 | 0.000003 |
Endoplasmic Reticulum | 0.006936 | 0.000835 | 0.000159 | 0.000191 | 0.000587 | 0.000611 | 0.000081 | 0.000133 | 0.000924 | 0.000696 | 0.000095 | 0.000166 |
Endosome | 0.006870 | 0.002116 | 0.000816 | 0.000928 | 0.000760 | 0.002246 | 0.000347 | 0.001254 | 0.001085 | 0.002197 | 0.000430 | 0.001067 |
Golgi | 0.101456 | 0.008308 | 0.035104 | 0.007230 | 0.007139 | 0.010146 | 0.003279 | 0.010960 | 0.012150 | 0.009449 | 0.008911 | 0.008824 |
Lipid Particles | 0.003701 | 0.001843 | 0.012878 | 0.010299 | 0.000517 | 0.000764 | 0.001047 | 0.020308 | 0.000686 | 0.001173 | 0.003141 | 0.014575 |
Mitochondria | 0.000031 | 0.000602 | 0.000137 | 0.000145 | 0.000123 | 0.000367 | 0.000103 | 0.000233 | 0.000118 | 0.000456 | 0.000109 | 0.000182 |
Mitotic Spindle | 0.003028 | 0.001424 | 0.004041 | 0.001815 | 0.001120 | 0.005330 | 0.001631 | 0.001127 | 0.001221 | 0.003848 | 0.002057 | 0.001521 |
None | 0.000584 | 0.001955 | 0.002677 | 0.002008 | 0.001593 | 0.000963 | 0.001675 | 0.002023 | 0.001539 | 0.001339 | 0.001853 | 0.002015 |
Nuclear Periphery | 0.000046 | 0.000040 | 0.001307 | 0.000133 | 0.000016 | 0.000040 | 0.000146 | 0.000218 | 0.000017 | 0.000040 | 0.000351 | 0.000169 |
Nuclear Periphery Foci | 0.004732 | 0.002829 | 0.013261 | 0.003144 | 0.000824 | 0.001481 | 0.001807 | 0.004695 | 0.001031 | 0.001993 | 0.003834 | 0.003807 |
Nucleolus | 0.000021 | 0.000204 | 0.000118 | 0.000134 | 0.000027 | 0.000040 | 0.000112 | 0.000182 | 0.000027 | 0.000102 | 0.000113 | 0.000155 |
Nucleus | 0.002581 | 0.006784 | 0.041382 | 0.020464 | 0.003940 | 0.004455 | 0.025669 | 0.019165 | 0.003868 | 0.005339 | 0.028450 | 0.019909 |
Peroxisomes | 0.000100 | 0.000214 | 0.000022 | 0.000108 | 0.000062 | 0.000128 | 0.000033 | 0.000077 | 0.000064 | 0.000161 | 0.000031 | 0.000095 |
Vacuole | 0.016661 | 0.012561 | 0.084954 | 0.082710 | 0.004673 | 0.011279 | 0.045743 | 0.135707 | 0.005310 | 0.011766 | 0.052682 | 0.105352 |
Vacuole Periphery | 0.033642 | 0.004137 | 0.023226 | 0.005693 | 0.004884 | 0.005066 | 0.007407 | 0.008110 | 0.006412 | 0.004713 | 0.010206 | 0.006726 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | -0.01 | 1.79 | -0.36 | -0.37 | -1.93 | -4.36 | 1.17 | -4.59 | -1.89 | -4.68 | -5.78 | -1.06 | -5.08 | -1.62 | -4.98 |
Bud Neck | -0.44 | -7.95 | -7.24 | -7.71 | 4.29 | -3.89 | -8.22 | -8.07 | -7.37 | -5.41 | -5.37 | -11.67 | -12.23 | -10.66 | -4.09 |
Bud Site | 2.94 | 4.39 | 2.35 | -1.21 | -5.10 | -5.67 | 7.46 | -4.42 | -0.80 | -6.81 | -5.61 | 7.78 | -5.52 | -1.57 | -9.29 |
Cell Periphery | 4.25 | -6.95 | -4.12 | -12.44 | 5.24 | -1.22 | -5.76 | -11.56 | -11.53 | -11.02 | 1.60 | -9.25 | -16.23 | -16.39 | -8.99 |
Cytoplasm | -7.62 | 0.29 | -3.67 | 14.61 | -8.23 | 6.56 | 16.57 | 24.89 | 20.43 | 16.60 | 3.47 | 18.52 | 26.68 | 24.26 | 11.11 |
Cytoplasmic Foci | 5.86 | 4.75 | 7.10 | 8.42 | 10.54 | -3.09 | 15.40 | 9.09 | 10.72 | -5.80 | 0.27 | 12.10 | 12.94 | 14.11 | 1.63 |
Eisosomes | 0.52 | -2.40 | -1.15 | -1.89 | 1.41 | -6.15 | 0.54 | -7.04 | -5.75 | -7.10 | -4.78 | -5.57 | -9.21 | -5.79 | -7.65 |
Endoplasmic Reticulum | 2.02 | 2.25 | 2.24 | 3.60 | -0.67 | -0.14 | 4.55 | 4.17 | 4.11 | -1.87 | 1.07 | 4.29 | 3.91 | 5.23 | -2.21 |
Endosome | 3.75 | 4.87 | 4.77 | 3.03 | -0.11 | -6.38 | 4.19 | -1.38 | 3.12 | -2.64 | -5.40 | 5.58 | 0.29 | 4.62 | -2.83 |
Golgi | 6.50 | 4.61 | 6.59 | 1.32 | 12.27 | -2.93 | 6.45 | -2.87 | 0.19 | -7.78 | 2.33 | 2.88 | 3.57 | 1.54 | 1.14 |
Lipid Particles | 2.01 | -6.79 | -6.01 | -9.30 | 2.12 | -2.28 | -5.00 | -11.81 | -11.63 | -11.23 | -2.98 | -10.09 | -16.08 | -15.31 | -12.00 |
Mitochondria | -1.61 | -10.30 | -3.17 | 1.17 | -0.49 | -1.85 | 0.26 | -1.69 | 0.91 | -1.74 | -2.14 | -0.83 | -2.15 | 1.55 | -1.96 |
Mitotic Spindle | 1.36 | -0.83 | 1.01 | -0.54 | 1.85 | -5.01 | -2.75 | -0.38 | 4.64 | 1.95 | -4.80 | -3.33 | -0.91 | 3.76 | 2.03 |
None | -3.75 | -9.19 | -3.49 | 0.01 | 1.74 | 2.08 | -0.20 | -0.45 | -1.76 | -0.38 | 0.66 | -0.96 | -0.88 | -1.74 | -0.22 |
Nuclear Periphery | 0.30 | -11.11 | -5.42 | -6.39 | 10.14 | -3.03 | -11.39 | -13.11 | -10.59 | -1.54 | -3.81 | -14.84 | -14.26 | -11.34 | 7.65 |
Nuclear Periphery Foci | 1.19 | -5.17 | 0.91 | -0.91 | 8.42 | -2.66 | -5.85 | -12.02 | -8.30 | -6.84 | -4.50 | -10.43 | -12.97 | -6.74 | 0.78 |
Nucleolus | -2.12 | -11.30 | -6.93 | 0.77 | -0.38 | -3.55 | -17.77 | -3.86 | -3.55 | -1.40 | -2.29 | -20.35 | -6.50 | -1.48 | -1.38 |
Nucleus | -5.34 | -14.73 | -14.03 | -12.15 | 6.66 | -1.54 | -22.93 | -14.49 | -14.15 | 4.94 | -4.56 | -26.91 | -19.73 | -18.48 | 6.90 |
Peroxisomes | -2.25 | 3.63 | -0.14 | 2.27 | -5.04 | -5.31 | 4.22 | -1.09 | 3.55 | -3.63 | -4.87 | 5.19 | -2.48 | 3.29 | -5.76 |
Vacuole | 0.86 | -10.61 | -13.37 | -17.68 | -0.29 | -6.43 | -20.88 | -23.44 | -22.15 | -14.67 | -7.93 | -24.61 | -30.11 | -28.00 | -13.39 |
Vacuole Periphery | 3.64 | 1.35 | 3.49 | -1.47 | 7.80 | -0.20 | -3.19 | -2.59 | -2.28 | -0.10 | 2.11 | -3.96 | 0.30 | -2.35 | 5.27 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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