Standard name | |
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Human Ortholog | |
Description | Protein of unknown function; overproduction blocks cell cycle arrest in the presence of mating pheromone; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | – | 0 | 1 | 1 | 0 | 0 | 1 | 1 | 3 | 1 | 4 | 2 | 5 | 1 | 0 | 4 | 14 | 4 | 2 | 10 | 12 | 21 |
Bud | 3 | 4 | – | 1 | 3 | 0 | 3 | 2 | 12 | 12 | 10 | 7 | 13 | 4 | 2 | 2 | 0 | 1 | 0 | 0 | 0 | 0 | 2 | 6 |
Bud Neck | 0 | 0 | – | 1 | 3 | 0 | 1 | 1 | 1 | 3 | 1 | 0 | 5 | 1 | 2 | 0 | 0 | 0 | 5 | 2 | 1 | 7 | 11 | 16 |
Bud Site | 0 | 0 | – | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 35 | 12 | – | 9 | 20 | 23 | 12 | 19 | 15 | 17 | 11 | 16 | 71 | 156 | 176 | 57 | 13 | 44 | 38 | 10 | 9 | 43 | 79 | 105 |
Cytoplasm | 248 | 96 | – | 121 | 227 | 200 | 285 | 313 | 207 | 241 | 181 | 183 | 294 | 446 | 514 | 112 | 15 | 87 | 189 | 77 | 15 | 194 | 304 | 303 |
Endoplasmic Reticulum | 2 | 1 | – | 0 | 0 | 0 | 0 | 0 | 3 | 1 | 0 | 0 | 2 | 1 | 0 | 5 | 0 | 5 | 3 | 2 | 0 | 4 | 3 | 4 |
Endosome | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 1 |
Golgi | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 1 | 1 | – | 1 | 1 | 15 | 47 | 33 | 57 | 72 | 86 | 68 | 0 | 0 | 0 | 1 | 0 | 1 | 2 | 1 | 5 | 3 | 4 | 4 |
Nucleus | 0 | 0 | – | 2 | 3 | 2 | 0 | 6 | 2 | 3 | 0 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 1 | 2 | – | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 1 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | – | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 2 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 1 |
Vac/Vac Membrane | 0 | 0 | – | 0 | 0 | 1 | 5 | 0 | 0 | 3 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 7 | 0 | 0 | 2 | 1 | 0 | 1 |
Unique Cell Count | 257 | 100 | 121 | 230 | 208 | 305 | 332 | 237 | 291 | 228 | 223 | 301 | 456 | 520 | 130 | 22 | 107 | 261 | 102 | 41 | 271 | 431 | 474 | |
Labelled Cell Count | 290 | 117 | 135 | 258 | 244 | 353 | 374 | 298 | 355 | 294 | 276 | 389 | 610 | 703 | 180 | 28 | 150 | 261 | 102 | 41 | 271 | 431 | 474 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 5.0 | 6.0 | – | 5.7 | 6.0 | 4.9 | 4.8 | 5.4 | 4.1 | 4.4 | 4.0 | 4.1 | 6.2 | 6.4 | 6.5 | 7.6 | 8.5 | 8.4 | 5.8 | 6.3 | 7.0 |
Std Deviation (1e-4) | 0.6 | 0.7 | – | 1.4 | 1.6 | 1.2 | 1.7 | 1.8 | 0.9 | 1.5 | 0.9 | 0.7 | 0.7 | 0.8 | 1.3 | 1.4 | 1.2 | 2.4 | 1.0 | 1.2 | 1.6 |
Intensity Change (Log2) | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Actin | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud Neck | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud Site | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cell Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cyto | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endoplasmic Reticulum | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endosome | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Golgi | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Mitochondria | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nuclear Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nuc | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleolus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Peroxisomes | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
SpindlePole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Vac | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cortical Patches | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cytoplasm | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Vacuole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 0.909 | 0.9673 | 0.5584 | 0.7267 | 0.3694 | 0.7261 | 0.889 | 1.6455 | 1.514 | 0.9594 | 1.3318 | 1.1017 | 0.9081 | 1.1171 | 1.1438 | 1.4981 | 1.4817 | 0.814 |
Actin | 0.0588 | 0.005 | 0.011 | 0.0101 | 0.0471 | 0.025 | 0.0326 | 0.0022 | 0.0144 | 0.0033 | 0.0247 | 0.0023 | 0.0172 | 0.0053 | 0.0203 | 0.0742 | 0.0276 | 0.0048 |
Bud | 0.0008 | 0.001 | 0.0014 | 0.0118 | 0.0004 | 0.002 | 0.0006 | 0.0026 | 0.0008 | 0.0303 | 0.0015 | 0.0002 | 0.0004 | 0.0005 | 0.0003 | 0.0021 | 0.0005 | 0.0003 |
Bud Neck | 0.0082 | 0.0046 | 0.005 | 0.0113 | 0.0026 | 0.0074 | 0.0016 | 0.0021 | 0.0046 | 0.0052 | 0.0211 | 0.0069 | 0.0046 | 0.002 | 0.003 | 0.0031 | 0.0026 | 0.0082 |
Bud Periphery | 0.0017 | 0.0012 | 0.0022 | 0.0268 | 0.0015 | 0.0053 | 0.0013 | 0.0063 | 0.0017 | 0.0127 | 0.0042 | 0.0008 | 0.0007 | 0.001 | 0.001 | 0.0082 | 0.0035 | 0.0011 |
Bud Site | 0.005 | 0.0452 | 0.0105 | 0.0126 | 0.0012 | 0.0023 | 0.0061 | 0.0348 | 0.0343 | 0.0318 | 0.0065 | 0.0004 | 0.0127 | 0.0527 | 0.0057 | 0.006 | 0.0015 | 0.0007 |
Cell Periphery | 0.0131 | 0.0113 | 0.0177 | 0.011 | 0.0081 | 0.0084 | 0.0171 | 0.0194 | 0.0225 | 0.0151 | 0.0151 | 0.0143 | 0.0186 | 0.0228 | 0.0198 | 0.015 | 0.0377 | 0.0115 |
Cytoplasm | 0.5505 | 0.6587 | 0.643 | 0.675 | 0.6951 | 0.6667 | 0.5806 | 0.6936 | 0.7206 | 0.6876 | 0.6739 | 0.8772 | 0.7159 | 0.7686 | 0.8054 | 0.6711 | 0.716 | 0.8424 |
Cytoplasmic Foci | 0.0258 | 0.008 | 0.0085 | 0.0109 | 0.006 | 0.0093 | 0.0208 | 0.0064 | 0.0038 | 0.0074 | 0.0098 | 0.003 | 0.0158 | 0.005 | 0.0055 | 0.0123 | 0.0031 | 0.0035 |
Eisosomes | 0.001 | 0.0011 | 0.0013 | 0.0005 | 0.0008 | 0.0009 | 0.0024 | 0.0005 | 0.0003 | 0.0002 | 0.0002 | 0.0001 | 0.0008 | 0.0013 | 0.0009 | 0.0012 | 0.0014 | 0.0004 |
Endoplasmic Reticulum | 0.0076 | 0.0046 | 0.0057 | 0.005 | 0.0063 | 0.0063 | 0.0032 | 0.002 | 0.002 | 0.0032 | 0.0014 | 0.0018 | 0.0055 | 0.0042 | 0.0057 | 0.0054 | 0.0099 | 0.0034 |
Endosome | 0.0174 | 0.0009 | 0.0042 | 0.007 | 0.0046 | 0.0082 | 0.0137 | 0.002 | 0.0033 | 0.0031 | 0.0376 | 0.0002 | 0.0075 | 0.0003 | 0.0023 | 0.0037 | 0.0195 | 0.0008 |
Golgi | 0.0072 | 0.0007 | 0.0034 | 0.0023 | 0.0048 | 0.0044 | 0.0076 | 0.0008 | 0.0031 | 0.0038 | 0.0095 | 0.0002 | 0.0055 | 0.0006 | 0.002 | 0.0072 | 0.0046 | 0.0006 |
Lipid Particles | 0.0098 | 0.0008 | 0.0012 | 0.0013 | 0.0021 | 0.002 | 0.0107 | 0.0012 | 0.0014 | 0.012 | 0.037 | 0.0003 | 0.0086 | 0.0007 | 0.0029 | 0.011 | 0.0019 | 0.0005 |
Mitochondria | 0.0019 | 0.0003 | 0.0074 | 0.0016 | 0.032 | 0.0058 | 0.0162 | 0.0004 | 0.0017 | 0.0161 | 0.0312 | 0.0001 | 0.0053 | 0.0002 | 0.0019 | 0.0035 | 0.0726 | 0.0001 |
None | 0.2648 | 0.2515 | 0.2703 | 0.2052 | 0.1813 | 0.2303 | 0.2576 | 0.2194 | 0.1826 | 0.1526 | 0.0784 | 0.0907 | 0.1512 | 0.1331 | 0.1163 | 0.132 | 0.0812 | 0.1204 |
Nuclear Periphery | 0.0049 | 0.0004 | 0.0008 | 0.0005 | 0.0017 | 0.0054 | 0.0006 | 0.0003 | 0.0002 | 0.0006 | 0.0007 | 0.0001 | 0.0078 | 0.0002 | 0.0007 | 0.0009 | 0.0105 | 0.0001 |
Nucleolus | 0.0008 | 0.0004 | 0.0015 | 0.0005 | 0.0002 | 0.0003 | 0.0002 | 0.0008 | 0.0001 | 0.0012 | 0.0032 | 0 | 0.0004 | 0.0001 | 0.0002 | 0.0002 | 0.0003 | 0 |
Nucleus | 0.0022 | 0.0014 | 0.0015 | 0.0011 | 0.0006 | 0.0041 | 0.0005 | 0.0011 | 0.0005 | 0.0038 | 0.002 | 0.0003 | 0.0041 | 0.0004 | 0.0008 | 0.0006 | 0.0018 | 0.0003 |
Peroxisomes | 0.0066 | 0.0004 | 0.0009 | 0.0018 | 0.0009 | 0.0032 | 0.0198 | 0.0007 | 0.0008 | 0.0061 | 0.0212 | 0 | 0.0142 | 0.0001 | 0.0026 | 0.0404 | 0.0004 | 0 |
Punctate Nuclear | 0.009 | 0.0012 | 0.0009 | 0.0008 | 0.0005 | 0.0009 | 0.0036 | 0.0023 | 0.0003 | 0.0009 | 0.0007 | 0.0007 | 0.0009 | 0.0002 | 0.0015 | 0.001 | 0.0022 | 0.0001 |
Vacuole | 0.0024 | 0.0015 | 0.0014 | 0.0025 | 0.0014 | 0.0012 | 0.0022 | 0.001 | 0.0009 | 0.0026 | 0.0149 | 0.0003 | 0.002 | 0.0006 | 0.001 | 0.0009 | 0.0008 | 0.0006 |
Vacuole Periphery | 0.0005 | 0.0001 | 0.0003 | 0.0004 | 0.0007 | 0.0004 | 0.001 | 0.0001 | 0.0001 | 0.0005 | 0.0051 | 0 | 0.0004 | 0 | 0.0001 | 0.0002 | 0.0006 | 0 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 14.5332 | 13.9912 | 10.382 | 19.7861 | 21.0171 | 8.9385 | 16.1674 | 15.3448 | 19.0457 | 22.3476 |
Translational Efficiency | 0.8562 | 0.727 | 1.0905 | 0.9291 | 0.5967 | 1.4074 | 0.6702 | 1.2128 | 0.8462 | 0.737 |
Dataset | Hit |
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Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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