Standard name | |
---|---|
Human Ortholog | |
Description | Protein adaptor between Myo4p and the She2p-mRNA complex; part of the mRNA localization machinery that restricts accumulation of certain proteins to the bud; also required for cortical ER inheritance |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 42 | 44 | 92 | 91 | 51 | 61 | 60 | 41 | 52 | 49 | 26 | 47 | 141 | 115 | 110 | 27 | 14 | 23 | 66 | 47 | 123 | 76 | 92 | 122 |
Bud | 25 | 20 | 36 | 54 | 65 | 41 | 56 | 59 | 46 | 70 | 54 | 68 | 44 | 51 | 44 | 12 | 6 | 4 | 41 | 31 | 51 | 68 | 76 | 74 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 7 | 6 | 10 | 13 | 13 | 22 |
Bud Site | 3 | 0 | 11 | 6 | 5 | 6 | 4 | 5 | 3 | 6 | 3 | 12 | 6 | 0 | 4 | 1 | 0 | 1 | – | – | – | – | – | – |
Cell Periphery | 11 | 2 | 30 | 29 | 14 | 12 | 22 | 31 | 32 | 38 | 30 | 45 | 50 | 38 | 63 | 4 | 6 | 11 | 9 | 3 | 11 | 3 | 6 | 11 |
Cytoplasm | 63 | 29 | 43 | 30 | 41 | 67 | 67 | 70 | 62 | 63 | 58 | 62 | 100 | 133 | 262 | 25 | 30 | 21 | 29 | 16 | 14 | 27 | 42 | 53 |
Endoplasmic Reticulum | 24 | 39 | 39 | 35 | 27 | 81 | 51 | 49 | 69 | 48 | 47 | 54 | 52 | 48 | 81 | 2 | 2 | 3 | 28 | 22 | 35 | 32 | 53 | 76 |
Endosome | 11 | 0 | 4 | 3 | 19 | 13 | 18 | 32 | 8 | 3 | 1 | 11 | 3 | 1 | 9 | 1 | 0 | 0 | 4 | 6 | 9 | 21 | 12 | 14 |
Golgi | 7 | 6 | 31 | 17 | 7 | 10 | 15 | 17 | 19 | 8 | 9 | 6 | 15 | 6 | 25 | 16 | 13 | 10 | 11 | 8 | 21 | 20 | 39 | 23 |
Mitochondria | 75 | 12 | 68 | 112 | 129 | 278 | 395 | 397 | 368 | 602 | 416 | 529 | 2 | 2 | 8 | 4 | 6 | 6 | 41 | 8 | 55 | 41 | 53 | 52 |
Nucleus | 1 | 0 | 1 | 1 | 0 | 0 | 1 | 1 | 0 | 2 | 3 | 2 | 0 | 1 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 2 | 1 | 2 |
Nuclear Periphery | 2 | 0 | 0 | 0 | 0 | 1 | 2 | 2 | 5 | 1 | 2 | 5 | 0 | 1 | 0 | 0 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 2 |
Nucleolus | 3 | 0 | 1 | 3 | 5 | 6 | 5 | 7 | 3 | 5 | 7 | 4 | 1 | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 0 |
Peroxisomes | 4 | 0 | 11 | 8 | 11 | 4 | 6 | 12 | 1 | 3 | 1 | 3 | 2 | 3 | 10 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 1 | 0 | 1 | 0 | 1 | 2 | 1 | 2 | 0 | 1 | 0 | 0 | 2 | 3 | 4 | 0 | 1 | 1 | 0 | 0 | 1 | 2 | 1 | 1 |
Vac/Vac Membrane | 11 | 4 | 8 | 8 | 24 | 18 | 35 | 65 | 42 | 31 | 31 | 45 | 8 | 13 | 24 | 8 | 8 | 5 | 10 | 7 | 8 | 21 | 21 | 19 |
Unique Cell Count | 210 | 121 | 258 | 231 | 283 | 435 | 550 | 598 | 528 | 706 | 528 | 667 | 291 | 299 | 504 | 71 | 67 | 59 | 256 | 164 | 350 | 339 | 420 | 483 |
Labelled Cell Count | 283 | 156 | 376 | 397 | 399 | 600 | 738 | 791 | 710 | 930 | 688 | 893 | 426 | 415 | 645 | 100 | 88 | 86 | 256 | 164 | 350 | 339 | 420 | 483 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 8.8 | 11.6 | 9.8 | 9.0 | 8.8 | 8.0 | 7.6 | 7.5 | 7.3 | 7.0 | 6.8 | 6.6 | 11.2 | 10.7 | 10.8 | 12.3 | 12.5 | 13.2 | 10.9 | 11.1 | 11.3 |
Std Deviation (1e-4) | 1.9 | 2.3 | 1.9 | 1.7 | 2.2 | 1.9 | 1.7 | 1.6 | 1.3 | 1.4 | 1.5 | 1.4 | 2.4 | 2.5 | 2.4 | 2.7 | 3.5 | 3.3 | 1.8 | 1.6 | 1.6 |
Intensity Change (Log2) | – | – | – | -0.11 | -0.15 | -0.29 | -0.37 | -0.38 | -0.43 | -0.48 | -0.52 | -0.56 | 0.19 | 0.12 | 0.14 | 0.33 | 0.36 | 0.44 | 0.16 | 0.18 | 0.21 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0.9 | -4.6 | -6.6 | -8.4 | -10.7 | -8.8 | -11.2 | -11.3 | -10.9 | 3.0 | 0.7 | -4.1 | 0.4 | -2.3 | 0.5 |
Bud | 2.7 | 2.7 | -1.8 | -1.6 | -1.7 | -2.3 | -1.8 | -1.5 | -1.6 | 0.4 | 1.0 | -2.2 | 0.6 | -1.1 | -1.5 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | -1.0 | -1.7 | -2.4 | 0 | 0 | 0 | 0 | 0 | -2.2 | -1.5 | -3.6 | -3.3 | 0 | 0 | 0 |
Cell Periphery | 0.3 | -2.8 | -4.7 | -4.1 | -3.4 | -2.7 | -3.4 | -2.9 | -2.4 | 1.8 | 0.4 | 0.3 | -1.5 | -0.6 | 1.4 |
Cytoplasm | -1.1 | -0.7 | -0.4 | -1.7 | -2.0 | -1.9 | -3.4 | -2.2 | -3.2 | 4.7 | 7.0 | 9.4 | 3.4 | 4.9 | 3.3 |
Endoplasmic Reticulum | 0 | -2.0 | 1.2 | -2.5 | -3.1 | -0.8 | -4.0 | -2.6 | -3.2 | 0.9 | 0.3 | 0.3 | -2.8 | -2.7 | -2.1 |
Endosome | 0 | 3.0 | 1.2 | 1.4 | 2.5 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | -1.7 | -4.3 | -5.2 | -5.3 | -5.4 | -4.5 | -7.6 | -6.2 | -7.7 | -2.9 | -4.7 | -3.5 | 2.2 | 1.6 | 1.0 |
Mitochondria | 5.1 | 4.6 | 9.6 | 12.2 | 10.8 | 11.5 | 17.6 | 14.2 | 15.1 | -9.0 | -9.1 | -10.8 | -3.7 | -3.0 | -2.7 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | -0.5 | -0.2 | -2.9 | -2.9 | -1.9 | 0 | 0 | 0 | 0 | -2.8 | -2.5 | -1.8 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0.2 | 2.6 | 0.7 | 1.9 | 3.7 | 2.6 | 0.9 | 1.7 | 2.1 | -0.2 | 0.8 | 1.1 | 0 | 0 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 7.1508 | 8.2755 | 7.6743 | 7.6168 | 6.3615 | 8.0053 | 5.1718 | 6.3799 | 6.3853 | 6.0309 | 5.6826 | 7.1535 | 6.0881 | 6.9375 | 5.8531 | 5.9721 | 4.8948 | 6.4683 |
Actin | 0.1717 | 0.1138 | 0.1044 | 0.0725 | 0.2083 | 0.188 | 0.2009 | 0.0803 | 0.1528 | 0.0515 | 0.1327 | 0.1735 | 0.2468 | 0.1146 | 0.1336 | 0.1352 | 0.1983 | 0.1824 |
Bud | 0.0764 | 0.0449 | 0.2712 | 0.1905 | 0.1207 | 0.0206 | 0.0578 | 0.0611 | 0.2827 | 0.2261 | 0.1971 | 0.0284 | 0.0906 | 0.0516 | 0.2881 | 0.1665 | 0.1326 | 0.0186 |
Bud Neck | 0.0083 | 0.0072 | 0.0083 | 0.0071 | 0.0608 | 0.2826 | 0.0083 | 0.0086 | 0.0147 | 0.0395 | 0.0025 | 0.2336 | 0.0129 | 0.0083 | 0.0103 | 0.04 | 0.0117 | 0.2612 |
Bud Periphery | 0.1169 | 0.057 | 0.3739 | 0.4725 | 0.358 | 0.0421 | 0.0682 | 0.0447 | 0.35 | 0.4455 | 0.4295 | 0.0734 | 0.1235 | 0.0384 | 0.3327 | 0.3431 | 0.263 | 0.0497 |
Bud Site | 0.1197 | 0.3625 | 0.1039 | 0.018 | 0.0164 | 0.018 | 0.128 | 0.4464 | 0.0886 | 0.0154 | 0.0113 | 0.0167 | 0.1295 | 0.5392 | 0.0908 | 0.0377 | 0.0157 | 0.0115 |
Cell Periphery | 0.025 | 0.005 | 0.0031 | 0.0075 | 0.0087 | 0.01 | 0.0049 | 0.0057 | 0.0034 | 0.0035 | 0.0025 | 0.0067 | 0.01 | 0.0044 | 0.0039 | 0.0062 | 0.0023 | 0.0072 |
Cytoplasm | 0.1829 | 0.2511 | 0.0587 | 0.1135 | 0.0732 | 0.1377 | 0.105 | 0.2617 | 0.0375 | 0.0853 | 0.0953 | 0.1523 | 0.1445 | 0.1614 | 0.0523 | 0.1272 | 0.0602 | 0.1097 |
Cytoplasmic Foci | 0.0398 | 0.0349 | 0.0119 | 0.011 | 0.0102 | 0.0503 | 0.0635 | 0.0315 | 0.0148 | 0.0114 | 0.0197 | 0.0376 | 0.0338 | 0.0242 | 0.0149 | 0.0092 | 0.0514 | 0.0595 |
Eisosomes | 0.002 | 0.0003 | 0.0001 | 0.0001 | 0.0007 | 0.0011 | 0.001 | 0.0003 | 0.0002 | 0.0001 | 0.0002 | 0.0008 | 0.001 | 0.0002 | 0.0003 | 0.0005 | 0.0004 | 0.0008 |
Endoplasmic Reticulum | 0.0327 | 0.0083 | 0.0031 | 0.0027 | 0.0138 | 0.0185 | 0.024 | 0.0029 | 0.0009 | 0.002 | 0.0029 | 0.0127 | 0.0316 | 0.0016 | 0.0077 | 0.0033 | 0.0043 | 0.0195 |
Endosome | 0.0579 | 0.0472 | 0.0171 | 0.0099 | 0.0157 | 0.05 | 0.1015 | 0.0182 | 0.0123 | 0.0161 | 0.0211 | 0.0814 | 0.0675 | 0.0162 | 0.0157 | 0.0268 | 0.0626 | 0.0854 |
Golgi | 0.0614 | 0.0347 | 0.0144 | 0.0029 | 0.0161 | 0.093 | 0.0859 | 0.0153 | 0.0162 | 0.028 | 0.0073 | 0.1052 | 0.0515 | 0.0172 | 0.0151 | 0.0259 | 0.0646 | 0.1283 |
Lipid Particles | 0.008 | 0.0011 | 0.002 | 0.0005 | 0.0037 | 0.0036 | 0.0261 | 0.0005 | 0.001 | 0.0043 | 0.0056 | 0.001 | 0.0051 | 0.0005 | 0.0058 | 0.0049 | 0.0642 | 0.0016 |
Mitochondria | 0.0382 | 0.008 | 0.0068 | 0.0607 | 0.0692 | 0.0621 | 0.0468 | 0.0088 | 0.0134 | 0.0471 | 0.0399 | 0.0556 | 0.0168 | 0.0112 | 0.0144 | 0.0273 | 0.0328 | 0.0494 |
None | 0.0107 | 0.0011 | 0.0102 | 0.0032 | 0.0017 | 0.0019 | 0.0042 | 0.0011 | 0.0005 | 0.0007 | 0.001 | 0.0008 | 0.0036 | 0.0004 | 0.0017 | 0.0014 | 0.001 | 0.0008 |
Nuclear Periphery | 0.0164 | 0.0021 | 0.0012 | 0.0019 | 0.0061 | 0.0007 | 0.0235 | 0.0005 | 0.0004 | 0.0017 | 0.0017 | 0.0008 | 0.0044 | 0.0005 | 0.002 | 0.0029 | 0.0042 | 0.0011 |
Nucleolus | 0.0007 | 0.0003 | 0.0001 | 0.0002 | 0.0003 | 0.0003 | 0.003 | 0.0004 | 0.0003 | 0.0003 | 0.0006 | 0.0014 | 0.0008 | 0.0003 | 0.0003 | 0.0009 | 0.0005 | 0.0002 |
Nucleus | 0.005 | 0.0022 | 0.0008 | 0.0018 | 0.0021 | 0.0009 | 0.0066 | 0.0012 | 0.0008 | 0.0019 | 0.0015 | 0.0026 | 0.0037 | 0.0009 | 0.0013 | 0.004 | 0.0014 | 0.001 |
Peroxisomes | 0.0077 | 0.0019 | 0.0023 | 0.0035 | 0.0063 | 0.0079 | 0.0162 | 0.0026 | 0.0035 | 0.0045 | 0.0071 | 0.0018 | 0.0078 | 0.0027 | 0.0022 | 0.0037 | 0.0122 | 0.003 |
Punctate Nuclear | 0.0057 | 0.0005 | 0.0002 | 0.0012 | 0.0004 | 0.0008 | 0.0095 | 0.0004 | 0.0004 | 0.0004 | 0.0003 | 0.0003 | 0.0016 | 0.0004 | 0.0015 | 0.0005 | 0.0015 | 0.0005 |
Vacuole | 0.0096 | 0.0142 | 0.0048 | 0.0151 | 0.0054 | 0.0074 | 0.0096 | 0.0071 | 0.0046 | 0.0117 | 0.0141 | 0.0091 | 0.0099 | 0.0049 | 0.0041 | 0.0257 | 0.01 | 0.006 |
Vacuole Periphery | 0.0034 | 0.0017 | 0.0013 | 0.0035 | 0.0022 | 0.0024 | 0.0054 | 0.0007 | 0.0009 | 0.0031 | 0.0061 | 0.0043 | 0.0029 | 0.0009 | 0.0011 | 0.007 | 0.0052 | 0.0028 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 32.3769 | 37.8188 | 25.7723 | 30.6391 | 30.7839 | 45.734 | 39.7993 | 30.2854 | 25.7064 | 35.069 |
Translational Efficiency | 1.4986 | 1.4983 | 1.5249 | 1.2303 | 1.3325 | 0.9409 | 1.1406 | 1.4524 | 1.5491 | 1.2908 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✔ |
Gene Expression | ✔ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
---|
Gene | Images |
---|