Standard name | |
---|---|
Human Ortholog | |
Description | Protein arginine N-methyltransferase; exhibits septin and Hsl1p-dependent localization to the bud neck in budded cells and periodic Hsl1p-dependent phosphorylation; required with Hsl1p, and Elm1p for the mother-bud neck recruitment, phosphorylation, and degradation of Swe1p; interacts directly with Swe1p; relocalizes away from bud neck upon DNA replication stress; human homolog PRMT5 can complement yeast hsl7 mutant |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 8 | 16 | 1 | 1 | 7 | 0 | 3 | 2 | 5 | 0 | 0 | 4 | 2 | 4 | 2 | 5 | 6 | 1 | 10 | 24 | 3 | 1 | 3 | 5 |
Bud | 6 | 27 | 2 | 7 | 11 | 11 | 9 | 12 | 21 | 14 | 17 | 18 | 12 | 7 | 5 | 1 | 1 | 0 | 7 | 14 | 2 | 0 | 5 | 5 |
Bud Neck | 2 | 1 | 3 | 4 | 4 | 6 | 4 | 11 | 7 | 2 | 5 | 5 | 0 | 0 | 0 | 0 | 1 | 0 | 25 | 46 | 8 | 2 | 2 | 1 |
Bud Site | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 2 | 4 | 7 | 2 | 5 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 3 | 2 | 5 | 2 | 2 | 3 | 3 | 2 | 1 | 0 | 0 | 0 | 1 | 2 | 0 | 9 | 5 | 2 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 120 | 482 | 182 | 143 | 152 | 120 | 149 | 162 | 102 | 107 | 112 | 107 | 244 | 489 | 505 | 85 | 77 | 47 | 97 | 408 | 146 | 262 | 391 | 379 |
Endoplasmic Reticulum | 7 | 6 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 2 | 2 | 8 | 3 | 3 | 0 | 2 | 4 | 2 | 8 | 10 |
Endosome | 2 | 9 | 0 | 2 | 3 | 1 | 6 | 1 | 0 | 0 | 2 | 0 | 6 | 19 | 8 | 4 | 4 | 0 | 6 | 26 | 2 | 1 | 1 | 2 |
Golgi | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 3 | 0 | 0 | 2 | 1 | 0 | 0 | 1 |
Mitochondria | 0 | 5 | 1 | 4 | 4 | 16 | 36 | 17 | 51 | 71 | 67 | 57 | 1 | 0 | 0 | 1 | 2 | 1 | 0 | 8 | 5 | 7 | 6 | 4 |
Nucleus | 0 | 7 | 1 | 2 | 0 | 1 | 3 | 4 | 2 | 5 | 0 | 2 | 0 | 0 | 1 | 0 | 0 | 1 | 0 | 3 | 3 | 0 | 1 | 3 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 1 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 2 | 0 | 0 | 0 | 1 | 0 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 1 | 1 | 0 | 0 | 2 | 0 | 1 | 2 | 2 | 1 | 3 | 1 | 1 | 3 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 0 |
SpindlePole | 1 | 2 | 2 | 8 | 14 | 5 | 19 | 13 | 13 | 7 | 3 | 15 | 0 | 2 | 0 | 1 | 0 | 0 | 3 | 8 | 0 | 0 | 2 | 0 |
Vac/Vac Membrane | 2 | 7 | 3 | 3 | 1 | 1 | 2 | 7 | 1 | 1 | 3 | 2 | 2 | 7 | 3 | 3 | 3 | 2 | 0 | 15 | 6 | 7 | 13 | 19 |
Unique Cell Count | 142 | 531 | 191 | 164 | 186 | 146 | 193 | 206 | 161 | 171 | 163 | 165 | 259 | 525 | 518 | 97 | 93 | 51 | 156 | 569 | 191 | 289 | 443 | 442 |
Labelled Cell Count | 152 | 567 | 201 | 176 | 200 | 164 | 236 | 237 | 209 | 215 | 215 | 218 | 271 | 537 | 527 | 120 | 106 | 57 | 156 | 569 | 191 | 289 | 443 | 442 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 6.0 | 5.6 | 6.2 | 5.6 | 5.8 | 4.8 | 5.1 | 5.4 | 4.0 | 3.9 | 3.7 | 4.4 | 5.6 | 5.9 | 5.9 | 8.8 | 9.2 | 7.4 | 5.4 | 5.8 | 6.3 |
Std Deviation (1e-4) | 1.4 | 0.7 | 1.9 | 1.7 | 1.4 | 1.6 | 1.9 | 2.0 | 1.0 | 1.3 | 0.9 | 1.4 | 1.0 | 0.9 | 1.0 | 1.7 | 2.2 | 0.9 | 0.9 | 1.0 | 1.1 |
Intensity Change (Log2) | – | – | – | -0.15 | -0.11 | -0.37 | -0.29 | -0.21 | -0.65 | -0.66 | -0.74 | -0.51 | -0.15 | -0.09 | -0.08 | 0.5 | 0.57 | 0.26 | -0.21 | -0.09 | 0.01 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 2.6 | 0 | 2.1 | 2.6 | 0 | 0 | 0 | 0 | 2.2 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 2.0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | -2.7 | -4.1 | -3.9 | -5.1 | -4.9 | -7.6 | -7.7 | -6.6 | -7.3 | -0.5 | -1.0 | 1.5 | -2.4 | -3.5 | -0.9 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2.7 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 0 | 6.0 | 3.7 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 3.1 | 0 | 3.8 | 2.7 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 3.2431 | 3.6255 | 3.2719 | 2.8378 | 3.2229 | 3.6099 | 1.2616 | 2.0337 | 1.6782 | 1.6558 | 1.5839 | 1.5414 | 0.7328 | 1.5867 | 0.9014 | 1.1917 | 0.9469 | 1.0385 |
Actin | 0.0585 | 0.0027 | 0.038 | 0.0114 | 0.037 | 0.0342 | 0.0496 | 0.0124 | 0.0314 | 0.0332 | 0.0205 | 0.0153 | 0.0805 | 0.0054 | 0.0172 | 0.033 | 0.0084 | 0.0244 |
Bud | 0.0045 | 0.003 | 0.0015 | 0.0017 | 0.0021 | 0.0005 | 0.011 | 0.0042 | 0.0072 | 0.0092 | 0.0187 | 0.0019 | 0.0043 | 0.0021 | 0.0014 | 0.0006 | 0.0018 | 0.0012 |
Bud Neck | 0.0057 | 0.0184 | 0.5221 | 0.69 | 0.6203 | 0.2025 | 0.0299 | 0.0177 | 0.4534 | 0.6147 | 0.5114 | 0.166 | 0.0424 | 0.0414 | 0.4705 | 0.695 | 0.5833 | 0.194 |
Bud Periphery | 0.0052 | 0.0014 | 0.0018 | 0.0021 | 0.0041 | 0.0008 | 0.0134 | 0.0046 | 0.0093 | 0.0256 | 0.0186 | 0.0044 | 0.0037 | 0.0013 | 0.0014 | 0.0006 | 0.0019 | 0.0014 |
Bud Site | 0.0448 | 0.1562 | 0.1866 | 0.0235 | 0.009 | 0.0017 | 0.104 | 0.227 | 0.1823 | 0.014 | 0.0362 | 0.0038 | 0.1496 | 0.0808 | 0.163 | 0.0202 | 0.0237 | 0.0077 |
Cell Periphery | 0.003 | 0.0014 | 0.0026 | 0.0027 | 0.0015 | 0.0006 | 0.001 | 0.0009 | 0.001 | 0.0004 | 0.0017 | 0.0001 | 0.0009 | 0.001 | 0.0008 | 0.0005 | 0.0004 | 0.0002 |
Cytoplasm | 0.3948 | 0.5303 | 0.1173 | 0.0497 | 0.2151 | 0.5139 | 0.4443 | 0.5601 | 0.1615 | 0.0946 | 0.1069 | 0.6644 | 0.4222 | 0.6235 | 0.1353 | 0.13 | 0.1523 | 0.6152 |
Cytoplasmic Foci | 0.0867 | 0.0381 | 0.0406 | 0.0398 | 0.088 | 0.0513 | 0.0901 | 0.0394 | 0.0615 | 0.0427 | 0.0767 | 0.0464 | 0.08 | 0.0161 | 0.055 | 0.0387 | 0.0324 | 0.0184 |
Eisosomes | 0.0009 | 0.0001 | 0.0005 | 0.0014 | 0.0001 | 0.0002 | 0.0005 | 0.0002 | 0.0002 | 0.0002 | 0.0001 | 0.0001 | 0.0003 | 0.0001 | 0.0003 | 0.0001 | 0.0001 | 0.0001 |
Endoplasmic Reticulum | 0.0118 | 0.0021 | 0.0007 | 0.0012 | 0.0002 | 0.0025 | 0.0079 | 0.0021 | 0.0012 | 0.0013 | 0.0036 | 0.0022 | 0.0048 | 0.0028 | 0.0026 | 0.0005 | 0.0018 | 0.0018 |
Endosome | 0.0278 | 0.002 | 0.0079 | 0.0189 | 0.0013 | 0.0102 | 0.0438 | 0.0142 | 0.0115 | 0.045 | 0.0458 | 0.0101 | 0.0174 | 0.0051 | 0.0121 | 0.0111 | 0.0281 | 0.0105 |
Golgi | 0.0122 | 0.0006 | 0.0086 | 0.0097 | 0.0032 | 0.0038 | 0.0149 | 0.003 | 0.0122 | 0.0302 | 0.0167 | 0.0037 | 0.0098 | 0.0014 | 0.0061 | 0.0121 | 0.0113 | 0.0041 |
Lipid Particles | 0.0148 | 0.0005 | 0.0071 | 0.0388 | 0.0005 | 0.0032 | 0.0178 | 0.0027 | 0.0084 | 0.0221 | 0.0259 | 0.0014 | 0.0094 | 0.0004 | 0.0028 | 0.0231 | 0.0095 | 0.0018 |
Mitochondria | 0.0038 | 0.0004 | 0.0063 | 0.0031 | 0.0009 | 0.0006 | 0.0059 | 0.0009 | 0.0087 | 0.031 | 0.0519 | 0.0043 | 0.0042 | 0.0121 | 0.0022 | 0.0049 | 0.0444 | 0.0014 |
None | 0.2392 | 0.2222 | 0.0303 | 0.0698 | 0.0021 | 0.1423 | 0.0955 | 0.086 | 0.0292 | 0.0067 | 0.0296 | 0.0578 | 0.1428 | 0.1989 | 0.1019 | 0.0039 | 0.0625 | 0.1102 |
Nuclear Periphery | 0.019 | 0.0008 | 0.0004 | 0.0005 | 0.0001 | 0.0066 | 0.0119 | 0.0014 | 0.0004 | 0.0013 | 0.0015 | 0.0015 | 0.0025 | 0.0007 | 0.0007 | 0.0001 | 0.0016 | 0.0004 |
Nucleolus | 0.0019 | 0.0004 | 0.0005 | 0.0025 | 0.0009 | 0.0004 | 0.0014 | 0.0006 | 0.0005 | 0.0011 | 0.0009 | 0.0002 | 0.0003 | 0.0002 | 0.0009 | 0.0006 | 0.0014 | 0.0001 |
Nucleus | 0.0069 | 0.0035 | 0.001 | 0.001 | 0.001 | 0.0034 | 0.0105 | 0.0042 | 0.0019 | 0.0019 | 0.0025 | 0.0037 | 0.0019 | 0.0018 | 0.0012 | 0.0005 | 0.0017 | 0.0011 |
Peroxisomes | 0.0174 | 0.0029 | 0.0226 | 0.0223 | 0.0039 | 0.006 | 0.0205 | 0.0036 | 0.0101 | 0.015 | 0.0232 | 0.0065 | 0.0135 | 0.0011 | 0.0196 | 0.022 | 0.0255 | 0.0032 |
Punctate Nuclear | 0.0368 | 0.0113 | 0.0027 | 0.0041 | 0.0082 | 0.014 | 0.0189 | 0.0104 | 0.0057 | 0.0032 | 0.0042 | 0.0045 | 0.0077 | 0.0009 | 0.0031 | 0.0008 | 0.0055 | 0.0007 |
Vacuole | 0.0032 | 0.0014 | 0.0009 | 0.0053 | 0.0005 | 0.0006 | 0.0057 | 0.0042 | 0.0021 | 0.005 | 0.0025 | 0.0013 | 0.0013 | 0.0029 | 0.0016 | 0.0012 | 0.0017 | 0.0017 |
Vacuole Periphery | 0.0013 | 0.0001 | 0.0002 | 0.0005 | 0.0001 | 0.0004 | 0.0016 | 0.0003 | 0.0003 | 0.0018 | 0.0011 | 0.0003 | 0.0005 | 0.0001 | 0.0002 | 0.0002 | 0.0007 | 0.0004 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 30.8722 | 34.1499 | 30.9392 | 33.6224 | 31.6762 | 25.7496 | 40.451 | 40.9187 | 32.8168 | 39.7223 |
Translational Efficiency | 0.3618 | 0.3967 | 0.3587 | 0.3116 | 0.3151 | 0.3289 | 0.327 | 0.2872 | 0.3473 | 0.3057 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✔ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
1543 | 1452 | 1391 | 1161 | 1784 | 1897 | 211 | 740 | 3327 | 3349 | 1602 | 1901 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 678.52 | 847.91 | 974.31 | 922.56 | 743.20 | 787.59 | 1058.01 | 955.27 | 713.20 | 813.74 | 985.33 | 935.29 |
Standard Deviation | 73.46 | 120.49 | 115.27 | 118.95 | 121.27 | 105.12 | 101.41 | 114.48 | 106.91 | 115.96 | 117.02 | 118.31 |
Intensity Change Log 2 | — | 0.321520 | 0.521990 | 0.443251 | — | 0.083694 | 0.509531 | 0.362158 | — | 0.202094 | 0.515490 | 0.401429 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.000437 | 0.000657 | 0.001089 | 0.004621 | 0.000480 | 0.000662 | 0.001077 | 0.001976 | 0.000460 | 0.000660 | 0.001087 | 0.003591 |
Bud Neck | 0.084935 | 0.108385 | 0.131827 | 0.127279 | 0.187446* | 0.145099 | 0.158545 | 0.140813 | 0.139904 | 0.129181 | 0.135346 | 0.132547 |
Bud Site | 0.039491 | 0.089576 | 0.209328* | 0.446550* | 0.073960 | 0.076167 | 0.289405* | 0.444846* | 0.057974 | 0.081981 | 0.219875* | 0.445887* |
Cell Periphery | 0.000179 | 0.000161 | 0.000233 | 0.000248 | 0.000255 | 0.000197 | 0.000293 | 0.000295 | 0.000220 | 0.000181 | 0.000241 | 0.000266 |
Cytoplasm | 0.465243* | 0.432313* | 0.290805* | 0.267315* | 0.426990* | 0.376215* | 0.228624* | 0.236415* | 0.444731* | 0.400537* | 0.282616* | 0.255287* |
Cytoplasmic Foci | 0.206772* | 0.148850 | 0.013500 | 0.025584 | 0.121632 | 0.137552 | 0.029078 | 0.024207 | 0.161118 | 0.142450 | 0.015552 | 0.025048 |
Eisosomes | 0.000244 | 0.000090 | 0.000066 | 0.000060 | 0.000161 | 0.000112 | 0.000126 | 0.000067 | 0.000199 | 0.000102 | 0.000074 | 0.000063 |
Endoplasmic Reticulum | 0.000518 | 0.001219 | 0.002936 | 0.001182 | 0.000841 | 0.000526 | 0.002168 | 0.000379 | 0.000691 | 0.000826 | 0.002835 | 0.000869 |
Endosome | 0.020134 | 0.026610 | 0.008323 | 0.007795 | 0.016329 | 0.037128 | 0.020118 | 0.009542 | 0.018094 | 0.032568 | 0.009876 | 0.008475 |
Golgi | 0.004342 | 0.005430 | 0.000442 | 0.002513 | 0.002199 | 0.005500 | 0.000575 | 0.002434 | 0.003193 | 0.005469 | 0.000460 | 0.002482 |
Lipid Particles | 0.004959 | 0.003165 | 0.001205 | 0.000348 | 0.003691 | 0.003957 | 0.001742 | 0.000505 | 0.004279 | 0.003614 | 0.001276 | 0.000409 |
Mitochondria | 0.006777 | 0.003932 | 0.000984 | 0.003926 | 0.002023 | 0.013384 | 0.000108 | 0.001378 | 0.004227 | 0.009286 | 0.000869 | 0.002934 |
Mitotic Spindle | 0.002202 | 0.011805 | 0.021356 | 0.017650 | 0.002698 | 0.011465 | 0.001410 | 0.021234 | 0.002468 | 0.011613 | 0.018729 | 0.019045 |
None | 0.001909 | 0.001710 | 0.003097 | 0.001118 | 0.007622 | 0.001688 | 0.000506 | 0.001668 | 0.004972 | 0.001698 | 0.002756 | 0.001332 |
Nuclear Periphery | 0.000147 | 0.000391 | 0.001314 | 0.000144 | 0.000240 | 0.000268 | 0.000591 | 0.000228 | 0.000197 | 0.000321 | 0.001218 | 0.000177 |
Nuclear Periphery Foci | 0.000405 | 0.001048 | 0.001762 | 0.000974 | 0.000575 | 0.000532 | 0.003571 | 0.001365 | 0.000496 | 0.000756 | 0.002000 | 0.001126 |
Nucleolus | 0.001423 | 0.001367 | 0.001165 | 0.000109 | 0.001335 | 0.001067 | 0.000281 | 0.000097 | 0.001376 | 0.001197 | 0.001049 | 0.000105 |
Nucleus | 0.067287 | 0.055460 | 0.216577* | 0.014215 | 0.079853 | 0.049641 | 0.064247 | 0.009238 | 0.074025 | 0.052164 | 0.196513* | 0.012278 |
Peroxisomes | 0.010099 | 0.015369 | 0.009846 | 0.014751 | 0.007994 | 0.014213 | 0.016570 | 0.016824 | 0.008970 | 0.014715 | 0.010732 | 0.015558 |
Vacuole | 0.081016 | 0.090594 | 0.083776 | 0.062246 | 0.062982 | 0.121637 | 0.180735 | 0.085316 | 0.071346 | 0.108178 | 0.096547 | 0.071226 |
Vacuole Periphery | 0.001480 | 0.001869 | 0.000369 | 0.001372 | 0.000694 | 0.002988 | 0.000228 | 0.001174 | 0.001059 | 0.002503 | 0.000351 | 0.001295 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | -2.90 | -7.09 | -4.85 | -4.52 | -3.92 | -1.11 | -3.27 | -5.52 | -4.25 | -2.97 | -2.04 | -7.35 | -5.93 | -5.40 | -4.51 |
Bud Neck | -2.87 | -0.09 | -0.23 | 2.67 | -0.14 | 4.71 | 7.04 | 10.41 | 6.34 | -0.25 | 2.09 | 8.77 | 8.99 | 7.11 | -0.42 |
Bud Site | -9.26 | -13.36 | -27.23* | -20.93 | -15.86 | -0.29 | -4.70 | -18.10 | -17.94 | -6.13 | -6.45 | -11.95 | -31.76 | -28.16 | -18.08 |
Cell Periphery | 1.17 | -1.86 | -2.63 | -3.59 | -0.63 | 2.91 | -0.09 | 1.29 | -1.58 | 1.04 | 2.98 | 0.54 | 0.03 | -3.20 | -0.55 |
Cytoplasm | 2.94 | 18.25 | 12.66 | 9.15 | -3.58 | 4.82 | 9.07 | 10.19 | 6.40 | -2.29 | 5.75 | 20.06 | 15.66 | 10.35 | -3.70 |
Cytoplasmic Foci | 8.56 | 37.83 | 33.05 | 23.76 | -6.68 | -2.81 | 19.34 | 23.02 | 24.25 | -0.30 | 4.42 | 45.04 | 38.98 | 33.80 | -7.17 |
Eisosomes | 10.73 | 12.34 | 12.49 | 4.11 | 1.05 | 3.78 | 1.94 | 7.20 | 7.74 | 5.72 | 9.98 | 12.45 | 13.76 | 8.62 | 3.24 |
Endoplasmic Reticulum | -2.93 | -15.84 | -1.33 | 0.16 | 6.99 | 2.07 | -6.08 | 1.68 | -0.77 | 6.80 | -1.11 | -16.00 | -0.81 | -0.31 | 10.28 |
Endosome | -3.12 | 9.58 | 9.38 | 11.98 | 1.09 | -10.42 | 0.96 | 6.67 | 14.66 | 2.91 | -10.01 | 10.86 | 11.69 | 19.31 | 1.91 |
Golgi | -1.26 | 12.53 | 3.85 | 3.90 | -1.95 | -5.22 | 8.12 | 1.43 | 5.39 | -2.50 | -4.48 | 15.03 | 3.52 | 6.18 | -2.83 |
Lipid Particles | 3.04 | 8.11 | 10.94 | 5.63 | 10.26 | -0.36 | 2.75 | 7.96 | 5.84 | 6.78 | 1.45 | 8.66 | 13.18 | 7.89 | 12.37 |
Mitochondria | 2.74 | 5.99 | 3.09 | 1.19 | -1.51 | -7.30 | 4.80 | 2.05 | 8.04 | -2.04 | -4.93 | 6.68 | 2.94 | 6.79 | -1.58 |
Mitotic Spindle | -4.60 | -7.47 | -6.02 | -2.43 | -0.19 | -5.06 | 0.80 | -4.45 | -1.80 | -4.71 | -6.86 | -7.39 | -7.46 | -3.08 | -0.81 |
None | 0.95 | -1.24 | 1.57 | 0.91 | 4.70 | 4.48 | 5.42 | 4.00 | -0.01 | -1.47 | 4.54 | 3.47 | 4.54 | 0.47 | 2.82 |
Nuclear Periphery | -2.83 | -21.90 | -7.77 | -0.61 | 18.34 | -0.76 | -10.19 | -3.64 | -2.85 | 7.15 | -2.90 | -22.60 | -7.84 | -2.45 | 19.20 |
Nuclear Periphery Foci | -2.57 | -8.07 | -3.24 | -0.77 | 4.00 | -0.32 | -8.62 | -2.97 | -1.92 | 6.16 | -1.79 | -11.53 | -4.42 | -2.03 | 5.83 |
Nucleolus | 0.02 | 0.85 | 2.72 | 2.88 | 4.12 | 1.19 | 4.57 | 5.06 | 5.21 | 4.00 | 0.62 | 1.54 | 4.88 | 4.85 | 4.26 |
Nucleus | 2.53 | -28.30 | 9.31 | 6.25 | 32.45 | 8.16 | -1.64 | 15.25 | 8.15 | 7.37 | 7.96 | -26.88 | 16.74 | 9.18 | 33.13 |
Peroxisomes | -3.57 | 3.90 | -0.37 | 2.83 | -3.49 | -4.96 | -0.46 | -2.08 | 1.23 | -0.88 | -6.01 | 2.88 | -1.96 | 2.94 | -3.94 |
Vacuole | -3.07 | -11.11 | -11.80 | -9.58 | -2.87 | -13.16 | -13.46 | -14.89 | -8.73 | 5.35 | -12.12 | -17.36 | -19.13 | -12.11 | -2.22 |
Vacuole Periphery | -1.37 | 6.38 | 1.18 | 1.87 | -1.14 | -9.49 | 9.01 | -0.12 | 5.54 | -1.57 | -7.85 | 8.35 | 0.72 | 4.65 | -1.59 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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