Standard name
Human Ortholog
Description Protein arginine N-methyltransferase; exhibits septin and Hsl1p-dependent localization to the bud neck in budded cells and periodic Hsl1p-dependent phosphorylation; required with Hsl1p, and Elm1p for the mother-bud neck recruitment, phosphorylation, and degradation of Swe1p; interacts directly with Swe1p; relocalizes away from bud neck upon DNA replication stress; human homolog PRMT5 can complement yeast hsl7 mutant
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0.06 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.06 0 0.07 0 0 0 0 0
Bud 0 0.05 0 0 0.06 0.08 0.05 0.06 0.13 0.08 0.1 0.11 0.05 0 0 0 0 0 0.05 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0.16 0.08 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.09 0.05 0 0 0 0 0 0 0
Cytoplasm 0.85 0.91 0.95 0.87 0.82 0.82 0.77 0.79 0.63 0.63 0.69 0.65 0.94 0.93 0.97 0.88 0.83 0.92 0.63 0.72 0.77 0.91 0.88 0.86
Endoplasmic Reticulum 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.08 0 0.06 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0.11 0.19 0.08 0.32 0.42 0.41 0.35 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0.05 0.08 0 0.1 0.06 0.08 0 0 0.09 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 8 16 1 1 7 0 3 2 5 0 0 4 2 4 2 5 6 1 10 24 3 1 3 5
Bud 6 27 2 7 11 11 9 12 21 14 17 18 12 7 5 1 1 0 7 14 2 0 5 5
Bud Neck 2 1 3 4 4 6 4 11 7 2 5 5 0 0 0 0 1 0 25 46 8 2 2 1
Bud Site 0 1 0 0 0 0 0 2 4 7 2 5 0 0 0 0 0 0 – – – – – –
Cell Periphery 3 2 5 2 2 3 3 2 1 0 0 0 1 2 0 9 5 2 0 0 0 0 0 0
Cytoplasm 120 482 182 143 152 120 149 162 102 107 112 107 244 489 505 85 77 47 97 408 146 262 391 379
Endoplasmic Reticulum 7 6 1 0 0 0 0 0 0 0 0 0 1 2 2 8 3 3 0 2 4 2 8 10
Endosome 2 9 0 2 3 1 6 1 0 0 2 0 6 19 8 4 4 0 6 26 2 1 1 2
Golgi 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 1 3 0 0 2 1 0 0 1
Mitochondria 0 5 1 4 4 16 36 17 51 71 67 57 1 0 0 1 2 1 0 8 5 7 6 4
Nucleus 0 7 1 2 0 1 3 4 2 5 0 2 0 0 1 0 0 1 0 3 3 0 1 3
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 1 1 1 1 0 1 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 1 2 0 0 0 1 0 1 1 1 1 0 0 0 0 0 0 0
Peroxisomes 1 1 0 0 2 0 1 2 2 1 3 1 1 3 0 0 0 0 1 1 0 0 0 0
SpindlePole 1 2 2 8 14 5 19 13 13 7 3 15 0 2 0 1 0 0 3 8 0 0 2 0
Vac/Vac Membrane 2 7 3 3 1 1 2 7 1 1 3 2 2 7 3 3 3 2 0 15 6 7 13 19
Unique Cell Count 142 531 191 164 186 146 193 206 161 171 163 165 259 525 518 97 93 51 156 569 191 289 443 442
Labelled Cell Count 152 567 201 176 200 164 236 237 209 215 215 218 271 537 527 120 106 57 156 569 191 289 443 442


Bud Neck, Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 6.0 5.6 6.2 5.6 5.8 4.8 5.1 5.4 4.0 3.9 3.7 4.4 5.6 5.9 5.9 8.8 9.2 7.4 5.4 5.8 6.3
Std Deviation (1e-4) 1.4 0.7 1.9 1.7 1.4 1.6 1.9 2.0 1.0 1.3 0.9 1.4 1.0 0.9 1.0 1.7 2.2 0.9 0.9 1.0 1.1
Intensity Change (Log2) – – – -0.15 -0.11 -0.37 -0.29 -0.21 -0.65 -0.66 -0.74 -0.51 -0.15 -0.09 -0.08 0.5 0.57 0.26 -0.21 -0.09 0.01

WT3RAP60RAP140RAP220RAP300RAP380RAP460RAP540RAP620RAP7000510WT3HU80HU120HU1600510WT3rpd3Δ_1rpd3Δ_2rpd3Δ_30510WT1AF100AF140AF1800510
rapamycinhydroxy-urearpd3 knockoutalpha-factorMultiple Condition Time-Course Intensity PlotsGFP Intensity (1e-4)GFP Intensity (1e-4)GFP Intensity (1e-4)GFP Intensity (1e-4)

Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 2.6 0 2.1 2.6 0 0 0 0 2.2 0 0 0 0 0
Bud Neck 0 0 0 0 2.0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm -2.7 -4.1 -3.9 -5.1 -4.9 -7.6 -7.7 -6.6 -7.3 -0.5 -1.0 1.5 -2.4 -3.5 -0.9
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 2.7 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 6.0 3.7 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 3.1 0 3.8 2.7 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 3.2431 3.6255 3.2719 2.8378 3.2229 3.6099 1.2616 2.0337 1.6782 1.6558 1.5839 1.5414 0.7328 1.5867 0.9014 1.1917 0.9469 1.0385
Actin 0.0585 0.0027 0.038 0.0114 0.037 0.0342 0.0496 0.0124 0.0314 0.0332 0.0205 0.0153 0.0805 0.0054 0.0172 0.033 0.0084 0.0244
Bud 0.0045 0.003 0.0015 0.0017 0.0021 0.0005 0.011 0.0042 0.0072 0.0092 0.0187 0.0019 0.0043 0.0021 0.0014 0.0006 0.0018 0.0012
Bud Neck 0.0057 0.0184 0.5221 0.69 0.6203 0.2025 0.0299 0.0177 0.4534 0.6147 0.5114 0.166 0.0424 0.0414 0.4705 0.695 0.5833 0.194
Bud Periphery 0.0052 0.0014 0.0018 0.0021 0.0041 0.0008 0.0134 0.0046 0.0093 0.0256 0.0186 0.0044 0.0037 0.0013 0.0014 0.0006 0.0019 0.0014
Bud Site 0.0448 0.1562 0.1866 0.0235 0.009 0.0017 0.104 0.227 0.1823 0.014 0.0362 0.0038 0.1496 0.0808 0.163 0.0202 0.0237 0.0077
Cell Periphery 0.003 0.0014 0.0026 0.0027 0.0015 0.0006 0.001 0.0009 0.001 0.0004 0.0017 0.0001 0.0009 0.001 0.0008 0.0005 0.0004 0.0002
Cytoplasm 0.3948 0.5303 0.1173 0.0497 0.2151 0.5139 0.4443 0.5601 0.1615 0.0946 0.1069 0.6644 0.4222 0.6235 0.1353 0.13 0.1523 0.6152
Cytoplasmic Foci 0.0867 0.0381 0.0406 0.0398 0.088 0.0513 0.0901 0.0394 0.0615 0.0427 0.0767 0.0464 0.08 0.0161 0.055 0.0387 0.0324 0.0184
Eisosomes 0.0009 0.0001 0.0005 0.0014 0.0001 0.0002 0.0005 0.0002 0.0002 0.0002 0.0001 0.0001 0.0003 0.0001 0.0003 0.0001 0.0001 0.0001
Endoplasmic Reticulum 0.0118 0.0021 0.0007 0.0012 0.0002 0.0025 0.0079 0.0021 0.0012 0.0013 0.0036 0.0022 0.0048 0.0028 0.0026 0.0005 0.0018 0.0018
Endosome 0.0278 0.002 0.0079 0.0189 0.0013 0.0102 0.0438 0.0142 0.0115 0.045 0.0458 0.0101 0.0174 0.0051 0.0121 0.0111 0.0281 0.0105
Golgi 0.0122 0.0006 0.0086 0.0097 0.0032 0.0038 0.0149 0.003 0.0122 0.0302 0.0167 0.0037 0.0098 0.0014 0.0061 0.0121 0.0113 0.0041
Lipid Particles 0.0148 0.0005 0.0071 0.0388 0.0005 0.0032 0.0178 0.0027 0.0084 0.0221 0.0259 0.0014 0.0094 0.0004 0.0028 0.0231 0.0095 0.0018
Mitochondria 0.0038 0.0004 0.0063 0.0031 0.0009 0.0006 0.0059 0.0009 0.0087 0.031 0.0519 0.0043 0.0042 0.0121 0.0022 0.0049 0.0444 0.0014
None 0.2392 0.2222 0.0303 0.0698 0.0021 0.1423 0.0955 0.086 0.0292 0.0067 0.0296 0.0578 0.1428 0.1989 0.1019 0.0039 0.0625 0.1102
Nuclear Periphery 0.019 0.0008 0.0004 0.0005 0.0001 0.0066 0.0119 0.0014 0.0004 0.0013 0.0015 0.0015 0.0025 0.0007 0.0007 0.0001 0.0016 0.0004
Nucleolus 0.0019 0.0004 0.0005 0.0025 0.0009 0.0004 0.0014 0.0006 0.0005 0.0011 0.0009 0.0002 0.0003 0.0002 0.0009 0.0006 0.0014 0.0001
Nucleus 0.0069 0.0035 0.001 0.001 0.001 0.0034 0.0105 0.0042 0.0019 0.0019 0.0025 0.0037 0.0019 0.0018 0.0012 0.0005 0.0017 0.0011
Peroxisomes 0.0174 0.0029 0.0226 0.0223 0.0039 0.006 0.0205 0.0036 0.0101 0.015 0.0232 0.0065 0.0135 0.0011 0.0196 0.022 0.0255 0.0032
Punctate Nuclear 0.0368 0.0113 0.0027 0.0041 0.0082 0.014 0.0189 0.0104 0.0057 0.0032 0.0042 0.0045 0.0077 0.0009 0.0031 0.0008 0.0055 0.0007
Vacuole 0.0032 0.0014 0.0009 0.0053 0.0005 0.0006 0.0057 0.0042 0.0021 0.005 0.0025 0.0013 0.0013 0.0029 0.0016 0.0012 0.0017 0.0017
Vacuole Periphery 0.0013 0.0001 0.0002 0.0005 0.0001 0.0004 0.0016 0.0003 0.0003 0.0018 0.0011 0.0003 0.0005 0.0001 0.0002 0.0002 0.0007 0.0004

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 30.8722 34.1499 30.9392 33.6224 31.6762 25.7496 40.451 40.9187 32.8168 39.7223
Translational Efficiency 0.3618 0.3967 0.3587 0.3116 0.3151 0.3289 0.327 0.2872 0.3473 0.3057

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✔
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1543 1452 1391 1161 1784 1897 211 740 3327 3349 1602 1901

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 678.52 847.91 974.31 922.56 743.20 787.59 1058.01 955.27 713.20 813.74 985.33 935.29
Standard Deviation 73.46 120.49 115.27 118.95 121.27 105.12 101.41 114.48 106.91 115.96 117.02 118.31
Intensity Change Log 2 — 0.321520 0.521990 0.443251 — 0.083694 0.509531 0.362158 — 0.202094 0.515490 0.401429

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000437 0.000657 0.001089 0.004621 0.000480 0.000662 0.001077 0.001976 0.000460 0.000660 0.001087 0.003591
Bud Neck 0.084935 0.108385 0.131827 0.127279 0.187446 0.145099 0.158545 0.140813 0.139904 0.129181 0.135346 0.132547
Bud Site 0.039491 0.089576 0.209328 0.446550 0.073960 0.076167 0.289405 0.444846 0.057974 0.081981 0.219875 0.445887
Cell Periphery 0.000179 0.000161 0.000233 0.000248 0.000255 0.000197 0.000293 0.000295 0.000220 0.000181 0.000241 0.000266
Cytoplasm 0.465243 0.432313 0.290805 0.267315 0.426990 0.376215 0.228624 0.236415 0.444731 0.400537 0.282616 0.255287
Cytoplasmic Foci 0.206772 0.148850 0.013500 0.025584 0.121632 0.137552 0.029078 0.024207 0.161118 0.142450 0.015552 0.025048
Eisosomes 0.000244 0.000090 0.000066 0.000060 0.000161 0.000112 0.000126 0.000067 0.000199 0.000102 0.000074 0.000063
Endoplasmic Reticulum 0.000518 0.001219 0.002936 0.001182 0.000841 0.000526 0.002168 0.000379 0.000691 0.000826 0.002835 0.000869
Endosome 0.020134 0.026610 0.008323 0.007795 0.016329 0.037128 0.020118 0.009542 0.018094 0.032568 0.009876 0.008475
Golgi 0.004342 0.005430 0.000442 0.002513 0.002199 0.005500 0.000575 0.002434 0.003193 0.005469 0.000460 0.002482
Lipid Particles 0.004959 0.003165 0.001205 0.000348 0.003691 0.003957 0.001742 0.000505 0.004279 0.003614 0.001276 0.000409
Mitochondria 0.006777 0.003932 0.000984 0.003926 0.002023 0.013384 0.000108 0.001378 0.004227 0.009286 0.000869 0.002934
Mitotic Spindle 0.002202 0.011805 0.021356 0.017650 0.002698 0.011465 0.001410 0.021234 0.002468 0.011613 0.018729 0.019045
None 0.001909 0.001710 0.003097 0.001118 0.007622 0.001688 0.000506 0.001668 0.004972 0.001698 0.002756 0.001332
Nuclear Periphery 0.000147 0.000391 0.001314 0.000144 0.000240 0.000268 0.000591 0.000228 0.000197 0.000321 0.001218 0.000177
Nuclear Periphery Foci 0.000405 0.001048 0.001762 0.000974 0.000575 0.000532 0.003571 0.001365 0.000496 0.000756 0.002000 0.001126
Nucleolus 0.001423 0.001367 0.001165 0.000109 0.001335 0.001067 0.000281 0.000097 0.001376 0.001197 0.001049 0.000105
Nucleus 0.067287 0.055460 0.216577 0.014215 0.079853 0.049641 0.064247 0.009238 0.074025 0.052164 0.196513 0.012278
Peroxisomes 0.010099 0.015369 0.009846 0.014751 0.007994 0.014213 0.016570 0.016824 0.008970 0.014715 0.010732 0.015558
Vacuole 0.081016 0.090594 0.083776 0.062246 0.062982 0.121637 0.180735 0.085316 0.071346 0.108178 0.096547 0.071226
Vacuole Periphery 0.001480 0.001869 0.000369 0.001372 0.000694 0.002988 0.000228 0.001174 0.001059 0.002503 0.000351 0.001295

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -2.90 -7.09 -4.85 -4.52 -3.92 -1.11 -3.27 -5.52 -4.25 -2.97 -2.04 -7.35 -5.93 -5.40 -4.51
Bud Neck -2.87 -0.09 -0.23 2.67 -0.14 4.71 7.04 10.41 6.34 -0.25 2.09 8.77 8.99 7.11 -0.42
Bud Site -9.26 -13.36 -27.23 -20.93 -15.86 -0.29 -4.70 -18.10 -17.94 -6.13 -6.45 -11.95 -31.76 -28.16 -18.08
Cell Periphery 1.17 -1.86 -2.63 -3.59 -0.63 2.91 -0.09 1.29 -1.58 1.04 2.98 0.54 0.03 -3.20 -0.55
Cytoplasm 2.94 18.25 12.66 9.15 -3.58 4.82 9.07 10.19 6.40 -2.29 5.75 20.06 15.66 10.35 -3.70
Cytoplasmic Foci 8.56 37.83 33.05 23.76 -6.68 -2.81 19.34 23.02 24.25 -0.30 4.42 45.04 38.98 33.80 -7.17
Eisosomes 10.73 12.34 12.49 4.11 1.05 3.78 1.94 7.20 7.74 5.72 9.98 12.45 13.76 8.62 3.24
Endoplasmic Reticulum -2.93 -15.84 -1.33 0.16 6.99 2.07 -6.08 1.68 -0.77 6.80 -1.11 -16.00 -0.81 -0.31 10.28
Endosome -3.12 9.58 9.38 11.98 1.09 -10.42 0.96 6.67 14.66 2.91 -10.01 10.86 11.69 19.31 1.91
Golgi -1.26 12.53 3.85 3.90 -1.95 -5.22 8.12 1.43 5.39 -2.50 -4.48 15.03 3.52 6.18 -2.83
Lipid Particles 3.04 8.11 10.94 5.63 10.26 -0.36 2.75 7.96 5.84 6.78 1.45 8.66 13.18 7.89 12.37
Mitochondria 2.74 5.99 3.09 1.19 -1.51 -7.30 4.80 2.05 8.04 -2.04 -4.93 6.68 2.94 6.79 -1.58
Mitotic Spindle -4.60 -7.47 -6.02 -2.43 -0.19 -5.06 0.80 -4.45 -1.80 -4.71 -6.86 -7.39 -7.46 -3.08 -0.81
None 0.95 -1.24 1.57 0.91 4.70 4.48 5.42 4.00 -0.01 -1.47 4.54 3.47 4.54 0.47 2.82
Nuclear Periphery -2.83 -21.90 -7.77 -0.61 18.34 -0.76 -10.19 -3.64 -2.85 7.15 -2.90 -22.60 -7.84 -2.45 19.20
Nuclear Periphery Foci -2.57 -8.07 -3.24 -0.77 4.00 -0.32 -8.62 -2.97 -1.92 6.16 -1.79 -11.53 -4.42 -2.03 5.83
Nucleolus 0.02 0.85 2.72 2.88 4.12 1.19 4.57 5.06 5.21 4.00 0.62 1.54 4.88 4.85 4.26
Nucleus 2.53 -28.30 9.31 6.25 32.45 8.16 -1.64 15.25 8.15 7.37 7.96 -26.88 16.74 9.18 33.13
Peroxisomes -3.57 3.90 -0.37 2.83 -3.49 -4.96 -0.46 -2.08 1.23 -0.88 -6.01 2.88 -1.96 2.94 -3.94
Vacuole -3.07 -11.11 -11.80 -9.58 -2.87 -13.16 -13.46 -14.89 -8.73 5.35 -12.12 -17.36 -19.13 -12.11 -2.22
Vacuole Periphery -1.37 6.38 1.18 1.87 -1.14 -9.49 9.01 -0.12 5.54 -1.57 -7.85 8.35 0.72 4.65 -1.59
Protein Complexes26S Proteasome complex37S mitochondrial small ribosomal subunit54S mitochondrial large ribosomal subunit60S cytosolic large ribosomal subunitAar2-U5 snRNPAnaphase-Promoting Complex variant 1Anaphase-Promoting Complex variant 2Anaphase-Promoting Complex variant 3Anaphase-Promoting Complex variant 4Arp2/3 complexATG1 kinase complexBLOC-1 complexCcr4-Not complexCentral kinetochore CTF19 complexCleavage and polyadenylation specificity factorcomplexCMG helicase complexCMG-Pol epsilon complexCOMPASS complexCOP9 signalosome COPI vesicle coat complexCoQ6 biosynthesis complexCore mediator complexCORVET complexCTF18-RFC complexCURI complex variant 1CURI complex variant 2CURI complex variant 3Cytoplasmic exosome complex, DIS3 variantDASH complexDNA-directed RNA Polymerase I complexDNA-directed RNA polymerase II complexDNA-directed RNA polymerase III complexDNA replication factor C complexEGO complexELG1-RFC complex Elongator holoenzyme complexEndoplasmic Reticulum Membrane ComplexEukaryotic translation initiation factor 2BcomplexEukaryotic translation initiation factor 3core complexExocystExomer complexFAR complexGeneral transcription factor complex TFIIDGeneral transcription factor complex TFIIHGET4-GET5 transmembrane domain recognition complexGID ubiquitin ligase complexGin4 complexGolgi transport complexGSE complexHMC complexHOPS complexINO80 chromatin remodeling complexLSM1-7 complexLsm1-7-Pat1 complexLSM2-7 complexLSM2-8 complexMCM complexMICOS complexMitochondrial electron transport complex IIIMitochondrial inner membrane pre-sequence translocasecomplexMitochondrial outer membrane translocase holocomplexMitochondrial proton-transporting ATP synthase complexMitochondrial pyruvate dehydrogenase complexNineTeen complexNOP8 60s ribosome pre-assembly complexNuA3 histone acetyltransferase complexNuA4 histone acetyltransferase complexNuclear condensin complexNuclear/nucleolar exosome complex, DIS3-RRP6 variantNuclear pore complexNucleolar ribonuclease MRP complexNucleolar ribonuclease P complexOligosaccharyl transferase complex variant 1Oligosaccharyl transferase complex variant 2PAF1 complexPAS complexPhosphatidylinositol 3-kinase complex IPolarisomePrefoldin complexPRP19-associated complexRad17 RFC-like complexRetromer complexRPD3L histone deacetylase complexRPD3S histone deacetylase complexRQC complexRSC complex variant RSC1RSC complex variant RSC2SAGA complexSCF-Met30 ubiquitin ligase complexSCF-Ufo1 ubiquitin ligase complexSEA complexSeptin complexSET3C histone deacetylase complexSignal recognition particleSLIK (SAGA-like) complexSmall ribosomal subunit processome, variant1Small ribosomal subunit processome, variant2Small ribosomal subunit processome, variant3SMC5-SMC6 SUMO ligase complexSm complexSpliceosomal commitment complexSPOTS complexSWI/SNF chromatin remodelling complexSwr1 chromatin remodelling complexTHO complexTranscription factor TFIIIC complexTranslocon complexTRAPPIII protein complexTRAPPII protein complexTRAPPI protein complexTREX complexU1 snRNPU2 snRNPU4 snRNPU4/U6 snRNPU4/U6.U5 tri-snRNP complexU5 snRNPU6 snRNP complexUTP-A complexUTP-B complexUTP-C complex variant 1UTP-C complex variant 2UTP-C complex variant 3Vacuolar proton translocating ATPase complex,Golgi variantVacuolar proton translocating ATPase complex,vacuole variantCellular Components••••••••••••••••actinbud neckcell peripherycytoplasmendosomeERGolgimitochondrionmixednuclear peripherynucleolusnucleusperoxisomespindle polevacuolar membranevacuole
Standard name
Human Ortholog
Description Protein arginine N-methyltransferase; exhibits septin and Hsl1p-dependent localization to the bud neck in budded cells and periodic Hsl1p-dependent phosphorylation; required with Hsl1p, and Elm1p for the mother-bud neck recruitment, phosphorylation, and degradation of Swe1p; interacts directly with Swe1p; relocalizes away from bud neck upon DNA replication stress; human homolog PRMT5 can complement yeast hsl7 mutant
Localization
Cell Percentages cytoplasm (61%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

Hsl7

Hsl7


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Hsl7-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available