Standard name | |
---|---|
Human Ortholog | |
Description | Nucleolar S-adenosylmethionine-dependent rRNA methyltransferase; methylates adenine (m1A) of the large subunit (LSU) rRNA at position 2142; belongs to Rossmann fold superfamily; green fluorescent protein (GFP)-fusion protein localizes to the nucleolus; YBR141C is not an essential gene |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 2 |
Bud | 0 | 0 | 1 | 3 | 3 | 7 | 15 | 13 | 14 | 17 | 15 | 18 | 2 | 1 | 1 | 3 | 0 | 0 | 3 | 1 | 1 | 2 | 7 | 11 |
Bud Neck | 0 | 0 | 0 | 16 | 9 | 12 | 8 | 15 | 8 | 15 | 7 | 15 | 6 | 22 | 9 | 0 | 0 | 0 | 0 | 1 | 2 | 3 | 12 | 12 |
Bud Site | 1 | 0 | 1 | 2 | 7 | 13 | 14 | 13 | 19 | 23 | 14 | 14 | 2 | 2 | 0 | 0 | 0 | 2 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 7 | 8 | 8 | 13 | 40 | 41 | 56 | 65 | 53 | 63 | 52 | 48 | 29 | 90 | 116 | 17 | 5 | 12 | 1 | 2 | 1 | 1 | 10 | 18 |
Endoplasmic Reticulum | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 7 | 3 | 3 | 0 | 0 | 0 | 0 | 2 | 2 |
Endosome | 0 | 2 | 1 | 6 | 14 | 14 | 4 | 4 | 3 | 1 | 1 | 1 | 9 | 60 | 117 | 12 | 6 | 9 | 16 | 11 | 4 | 35 | 87 | 103 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 1 | 2 | 2 | 5 | 1 | 1 | 0 | 1 | 1 | 4 |
Mitochondria | 183 | 15 | 13 | 11 | 21 | 89 | 140 | 73 | 198 | 155 | 227 | 136 | 17 | 10 | 12 | 18 | 8 | 28 | 7 | 0 | 4 | 7 | 27 | 21 |
Nucleus | 228 | 109 | 107 | 28 | 63 | 34 | 28 | 19 | 28 | 38 | 33 | 23 | 117 | 245 | 151 | 79 | 52 | 51 | 125 | 78 | 55 | 48 | 49 | 31 |
Nuclear Periphery | 11 | 3 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 23 | 2 | 5 | 0 | 8 | 8 |
Nucleolus | 78 | 35 | 10 | 88 | 22 | 28 | 16 | 13 | 16 | 19 | 15 | 8 | 125 | 298 | 121 | 76 | 59 | 93 | 157 | 72 | 54 | 123 | 159 | 155 |
Peroxisomes | 0 | 0 | 0 | 0 | 1 | 1 | 2 | 0 | 1 | 0 | 0 | 1 | 0 | 2 | 7 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 2 | 1 |
SpindlePole | 7 | 2 | 22 | 122 | 76 | 85 | 83 | 80 | 65 | 75 | 48 | 74 | 133 | 382 | 267 | 39 | 8 | 17 | 23 | 6 | 15 | 73 | 120 | 129 |
Vac/Vac Membrane | 19 | 8 | 3 | 1 | 10 | 6 | 3 | 3 | 4 | 4 | 8 | 6 | 2 | 4 | 13 | 11 | 6 | 12 | 29 | 18 | 13 | 20 | 40 | 52 |
Unique Cell Count | 373 | 160 | 139 | 177 | 203 | 231 | 259 | 227 | 281 | 283 | 292 | 254 | 300 | 742 | 564 | 195 | 118 | 172 | 394 | 198 | 161 | 326 | 547 | 581 |
Labelled Cell Count | 535 | 182 | 167 | 290 | 267 | 330 | 369 | 300 | 409 | 410 | 420 | 344 | 442 | 1120 | 816 | 267 | 150 | 233 | 394 | 198 | 161 | 326 | 547 | 581 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 4.3 | 5.9 | 5.2 | 5.3 | 5.5 | 4.3 | 3.8 | 4.4 | 3.2 | 3.5 | 3.1 | 3.5 | 5.5 | 5.6 | 5.5 | 8.2 | 7.7 | 7.3 | 5.9 | 5.6 | 6.1 |
Std Deviation (1e-4) | 0.6 | 1.2 | 0.8 | 1.7 | 2.6 | 2.3 | 2.0 | 2.0 | 0.9 | 1.0 | 0.9 | 0.7 | 1.1 | 1.0 | 1.9 | 1.7 | 1.7 | 1.9 | 1.5 | 1.9 | 2.0 |
Intensity Change (Log2) | – | – | – | 0.02 | 0.07 | -0.28 | -0.44 | -0.24 | -0.7 | -0.55 | -0.75 | -0.58 | 0.08 | 0.11 | 0.08 | 0.66 | 0.57 | 0.5 | 0.18 | 0.12 | 0.23 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 3.6 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 0.6 | 3.6 | 3.3 | 4.1 | 5.3 | 3.6 | 4.3 | 3.4 | 3.6 | 1.4 | 2.2 | 4.1 | 1.0 | -0.6 | 0.4 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 2.7 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3.1 | 5.7 | 2.5 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | -1.0 | 0.3 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | -1.4 | 0 | 0 | 0 | -0.8 | 1.8 |
Nucleus | -10.9 | -8.3 | -11.9 | -13.3 | -13.4 | -13.8 | -12.9 | -13.6 | -13.7 | -7.4 | -9.7 | -11.0 | -6.6 | -5.4 | -8.3 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 8.1 | 1.1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 7.3 | 7.5 | 3.9 | 6.5 | 7.7 | 8.7 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 9.4 | 4.3 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 5.8 | 7.7 | 6.8 | 1.0 | -2.3 | -1.6 |
Vacuole | 0 | 1.3 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1.6 | 0 | 2.0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 0.4931 | 0.6998 | 0.5234 | 0.1875 | 0.0437 | 0.4062 | 0.4229 | 1.0461 | 0.9916 | 0.7874 | 0.767 | 0.992 | 1.0347 | 1.1208 | 0.6901 | 1.2231 | 1.352 | 0.7453 |
Actin | 0.0142 | 0 | 0.0004 | 0 | 0 | 0.0125 | 0.0048 | 0.0051 | 0.0133 | 0.0013 | 0.0084 | 0.0036 | 0.0215 | 0 | 0.0087 | 0.0577 | 0.0058 | 0.0002 |
Bud | 0.0005 | 0.0001 | 0.0002 | 0.0001 | 0.0001 | 0.0002 | 0.0004 | 0.0005 | 0.0001 | 0.0002 | 0.0001 | 0.0001 | 0.0004 | 0.0001 | 0.0002 | 0.0003 | 0.0002 | 0.0001 |
Bud Neck | 0.0071 | 0.0017 | 0.002 | 0.0006 | 0.0034 | 0.0047 | 0.003 | 0.002 | 0.002 | 0.0059 | 0.002 | 0.0114 | 0.0021 | 0.0054 | 0.0029 | 0.0017 | 0.0015 | 0.0039 |
Bud Periphery | 0.001 | 0.0002 | 0.0005 | 0.0002 | 0.0002 | 0.0004 | 0.0013 | 0.0003 | 0.0003 | 0.0007 | 0.0006 | 0.0004 | 0.001 | 0.0002 | 0.0004 | 0.0006 | 0.0005 | 0.0003 |
Bud Site | 0.0084 | 0.0016 | 0.0026 | 0.0005 | 0.0012 | 0.0007 | 0.0034 | 0.0015 | 0.003 | 0.0008 | 0.0003 | 0.0007 | 0.0025 | 0.0014 | 0.0025 | 0.0013 | 0.0031 | 0.0006 |
Cell Periphery | 0.0004 | 0.0004 | 0.0004 | 0.0001 | 0.0001 | 0.0002 | 0.001 | 0.0007 | 0.0003 | 0.0009 | 0.0001 | 0.0002 | 0.0004 | 0.0006 | 0.0003 | 0.0005 | 0.0002 | 0.0002 |
Cytoplasm | 0.006 | 0.0005 | 0.0009 | 0.0001 | 0.0001 | 0.0122 | 0.0159 | 0.0022 | 0.0007 | 0.0015 | 0.0026 | 0.0003 | 0.0013 | 0.0022 | 0.0003 | 0.0002 | 0.0006 | 0.0001 |
Cytoplasmic Foci | 0.0177 | 0.0065 | 0.0059 | 0.0002 | 0.001 | 0.0073 | 0.0066 | 0.0033 | 0.0031 | 0.0036 | 0.002 | 0.0072 | 0.0187 | 0.0078 | 0.0116 | 0.0069 | 0.0116 | 0.0024 |
Eisosomes | 0.0005 | 0 | 0 | 0 | 0 | 0.0001 | 0.0002 | 0 | 0.0003 | 0.0001 | 0.0003 | 0.0001 | 0.0003 | 0 | 0.0001 | 0.0001 | 0.0001 | 0 |
Endoplasmic Reticulum | 0.0029 | 0.0002 | 0.0005 | 0 | 0 | 0.0004 | 0.0021 | 0.0006 | 0.0005 | 0.0032 | 0.0044 | 0.0003 | 0.0015 | 0.0001 | 0.0004 | 0.0002 | 0.0009 | 0.0001 |
Endosome | 0.0173 | 0.0041 | 0.0066 | 0.0001 | 0.0003 | 0.0055 | 0.0096 | 0.0046 | 0.002 | 0.0337 | 0.0181 | 0.0062 | 0.024 | 0.0019 | 0.0053 | 0.0083 | 0.0285 | 0.0016 |
Golgi | 0.0044 | 0.0006 | 0.0017 | 0 | 0.0002 | 0.0025 | 0.0041 | 0.0008 | 0.0013 | 0.0117 | 0.0054 | 0.0033 | 0.011 | 0.0008 | 0.0029 | 0.0245 | 0.0148 | 0.0008 |
Lipid Particles | 0.0212 | 0.018 | 0.0238 | 0.0011 | 0.0015 | 0.0278 | 0.0249 | 0.0193 | 0.0153 | 0.0606 | 0.0451 | 0.05 | 0.0627 | 0.0303 | 0.0227 | 0.0435 | 0.0677 | 0.0131 |
Mitochondria | 0.0083 | 0.0002 | 0.0015 | 0.0003 | 0.0002 | 0.0029 | 0.0192 | 0.0006 | 0.0016 | 0.0576 | 0.0392 | 0.0011 | 0.0104 | 0.0003 | 0.0085 | 0.0089 | 0.0049 | 0.0021 |
None | 0.0035 | 0.0003 | 0.0021 | 0.0001 | 0.0002 | 0.0032 | 0.0006 | 0.0018 | 0.0013 | 0.0054 | 0.0073 | 0.0005 | 0.0031 | 0.0128 | 0.0003 | 0.0003 | 0.0013 | 0.0002 |
Nuclear Periphery | 0.0267 | 0.0062 | 0.0079 | 0.0003 | 0.0027 | 0.0078 | 0.0033 | 0.0066 | 0.0075 | 0.0241 | 0.0667 | 0.0131 | 0.015 | 0.0037 | 0.0028 | 0.0041 | 0.0515 | 0.0062 |
Nucleolus | 0.5281 | 0.6829 | 0.7739 | 0.6054 | 0.6274 | 0.5824 | 0.721 | 0.7726 | 0.8183 | 0.5731 | 0.4115 | 0.6555 | 0.6373 | 0.7068 | 0.7759 | 0.647 | 0.542 | 0.7409 |
Nucleus | 0.1673 | 0.2086 | 0.1028 | 0.3879 | 0.2395 | 0.1847 | 0.1103 | 0.1318 | 0.1028 | 0.1838 | 0.2401 | 0.1438 | 0.0952 | 0.1666 | 0.1183 | 0.1521 | 0.1646 | 0.1415 |
Peroxisomes | 0.0092 | 0.0013 | 0.0018 | 0.0001 | 0.0002 | 0.0027 | 0.0067 | 0.0007 | 0.0028 | 0.0015 | 0.0032 | 0.0041 | 0.0121 | 0.0008 | 0.011 | 0.0026 | 0.0138 | 0.0011 |
Punctate Nuclear | 0.1487 | 0.06 | 0.06 | 0.0023 | 0.1211 | 0.1376 | 0.0522 | 0.0316 | 0.0217 | 0.0132 | 0.1368 | 0.0964 | 0.0747 | 0.053 | 0.0231 | 0.0356 | 0.0829 | 0.0827 |
Vacuole | 0.004 | 0.0061 | 0.0037 | 0.0003 | 0.0004 | 0.0024 | 0.0052 | 0.0112 | 0.0013 | 0.0118 | 0.0013 | 0.0012 | 0.003 | 0.0044 | 0.0015 | 0.0029 | 0.0019 | 0.0011 |
Vacuole Periphery | 0.0024 | 0.0005 | 0.0007 | 0.0001 | 0.0001 | 0.0018 | 0.0041 | 0.002 | 0.0004 | 0.0053 | 0.0045 | 0.0006 | 0.0018 | 0.0007 | 0.0004 | 0.0008 | 0.0015 | 0.0007 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 16.8666 | 5.8365 | 9.3838 | 11.6337 | 19.0466 | 9.7881 | 7.1308 | 7.6341 | 7.9699 | 12.0333 |
Translational Efficiency | 1.2252 | 2.4897 | 1.4081 | 0.9169 | 0.7514 | 1.3378 | 1.6277 | 1.2917 | 0.9287 | 0.9425 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✔ |
Gene Expression | ✔ |
Translational Efficiency | ✔ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
414 | 869 | 1097 | 241 | 1408 | 2079 | 247 | 1246 | 1822 | 2948 | 1344 | 1487 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 759.30 | 737.85 | 891.22 | 949.09 | 720.59 | 803.86 | 960.12 | 1014.10 | 729.39 | 784.40 | 903.88 | 1003.56 |
Standard Deviation | 96.74 | 106.89 | 127.28 | 156.00 | 89.73 | 110.11 | 116.23 | 155.75 | 92.80 | 113.24 | 128.13 | 157.62 |
Intensity Change Log 2 | — | -0.041342 | 0.231112 | 0.321875 | — | 0.157766 | 0.414036 | 0.492949 | — | 0.059041 | 0.323080 | 0.407710 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.000290 | 0.000798 | 0.000711 | 0.001020 | 0.000153 | 0.000608 | 0.000687 | 0.000862 | 0.000184 | 0.000664 | 0.000707 | 0.000888 |
Bud Neck | 0.002359 | 0.015985 | 0.019461 | 0.032800 | 0.004544 | 0.008235 | 0.005848 | 0.044732 | 0.004047 | 0.010520 | 0.016959 | 0.042798 |
Bud Site | 0.000286 | 0.004847 | 0.001331 | 0.004506 | 0.000459 | 0.002000 | 0.000750 | 0.009221 | 0.000420 | 0.002839 | 0.001224 | 0.008456 |
Cell Periphery | 0.000076 | 0.000232 | 0.000052 | 0.000138 | 0.000079 | 0.000097 | 0.000046 | 0.000162 | 0.000078 | 0.000137 | 0.000051 | 0.000158 |
Cytoplasm | 0.004880 | 0.023994 | 0.002432 | 0.011324 | 0.010794 | 0.005124 | 0.003133 | 0.008075 | 0.009450 | 0.010686 | 0.002561 | 0.008602 |
Cytoplasmic Foci | 0.003725 | 0.008080 | 0.000156 | 0.000640 | 0.003568 | 0.003320 | 0.001146 | 0.004382 | 0.003604 | 0.004723 | 0.000338 | 0.003776 |
Eisosomes | 0.000047 | 0.000083 | 0.000038 | 0.000053 | 0.000054 | 0.000058 | 0.000073 | 0.000055 | 0.000052 | 0.000066 | 0.000045 | 0.000054 |
Endoplasmic Reticulum | 0.003260 | 0.007544 | 0.003610 | 0.004880 | 0.001946 | 0.004495 | 0.004179 | 0.003969 | 0.002245 | 0.005393 | 0.003715 | 0.004117 |
Endosome | 0.001266 | 0.007653 | 0.000851 | 0.002870 | 0.001857 | 0.004749 | 0.000702 | 0.007511 | 0.001723 | 0.005605 | 0.000823 | 0.006758 |
Golgi | 0.001581 | 0.002917 | 0.000373 | 0.000839 | 0.000610 | 0.001431 | 0.000293 | 0.005112 | 0.000831 | 0.001869 | 0.000359 | 0.004420 |
Lipid Particles | 0.003288 | 0.003947 | 0.000400 | 0.000592 | 0.003630 | 0.003041 | 0.000921 | 0.000465 | 0.003552 | 0.003308 | 0.000496 | 0.000485 |
Mitochondria | 0.003832 | 0.012670 | 0.001725 | 0.003762 | 0.003833 | 0.009913 | 0.001057 | 0.007971 | 0.003833 | 0.010726 | 0.001602 | 0.007289 |
Mitotic Spindle | 0.005180 | 0.025192 | 0.011987 | 0.097935 | 0.004983 | 0.010794 | 0.006847 | 0.064631 | 0.005028 | 0.015039 | 0.011042 | 0.070028 |
None | 0.004016 | 0.021158 | 0.002298 | 0.005760 | 0.010624 | 0.004227 | 0.007305 | 0.004650 | 0.009123 | 0.009218 | 0.003218 | 0.004830 |
Nuclear Periphery | 0.013150 | 0.008669 | 0.002884 | 0.013091 | 0.004544 | 0.010647 | 0.001452 | 0.013262 | 0.006500 | 0.010064 | 0.002621 | 0.013234 |
Nuclear Periphery Foci | 0.010683 | 0.028035 | 0.006675 | 0.014865 | 0.019723 | 0.017021 | 0.004506 | 0.010591 | 0.017669 | 0.020268 | 0.006277 | 0.011284 |
Nucleolus | 0.347393* | 0.325832* | 0.404667* | 0.379677* | 0.515710* | 0.405804* | 0.332854* | 0.419814* | 0.477465* | 0.382230* | 0.391469* | 0.413309* |
Nucleus | 0.590121* | 0.486573* | 0.537034* | 0.410651* | 0.410002* | 0.500264* | 0.621716* | 0.370108* | 0.450929* | 0.496228* | 0.552597* | 0.376679* |
Peroxisomes | 0.002898 | 0.005301 | 0.001062 | 0.002168 | 0.001649 | 0.002397 | 0.002120 | 0.002298 | 0.001933 | 0.003253 | 0.001256 | 0.002277 |
Vacuole | 0.001204 | 0.006891 | 0.001637 | 0.008262 | 0.000759 | 0.003532 | 0.003958 | 0.013937 | 0.000860 | 0.004522 | 0.002064 | 0.013017 |
Vacuole Periphery | 0.000464 | 0.003599 | 0.000615 | 0.004167 | 0.000479 | 0.002244 | 0.000407 | 0.008193 | 0.000475 | 0.002643 | 0.000577 | 0.007540 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | -8.14 | -7.94 | -8.20 | -3.18 | -2.92 | -1.75 | -5.50 | -13.16 | -2.04 | -1.87 | -2.64 | -12.58 | -14.66 | -2.51 | -3.79 |
Bud Neck | -9.61 | -16.47 | -9.66 | -6.24 | -4.61 | -5.44 | -3.27 | -22.86 | -21.13 | -20.60 | -10.15 | -15.79 | -24.79 | -21.34 | -15.56 |
Bud Site | -6.24 | -8.54 | -6.21 | -1.73 | -4.34 | -2.71 | -3.73 | -10.41 | -8.53 | -9.57 | -5.38 | -8.58 | -11.29 | -8.45 | -9.63 |
Cell Periphery | -3.17 | 0.53 | -1.69 | 2.21 | -4.25 | -0.72 | 1.69 | -3.73 | -1.95 | -5.15 | -2.64 | 1.62 | -3.95 | -0.67 | -6.04 |
Cytoplasm | -5.49 | 1.60 | -1.90 | 2.79 | -3.05 | 3.28 | 4.50 | -0.04 | -3.09 | -4.23 | -0.75 | 5.63 | -0.83 | -0.19 | -5.76 |
Cytoplasmic Foci | -2.59 | 3.08 | 2.58 | 5.97 | -2.95 | 0.81 | 4.58 | 1.25 | 0.56 | -2.56 | -1.17 | 7.18 | 1.83 | 2.93 | -4.09 |
Eisosomes | -6.59 | 1.61 | -1.13 | 5.96 | -3.41 | -1.00 | -4.40 | -0.87 | 0.13 | 3.94 | -4.74 | 2.26 | -1.39 | 3.29 | -3.66 |
Endoplasmic Reticulum | -6.34 | -1.44 | -2.32 | 2.59 | -1.66 | -8.75 | -2.71 | -6.36 | 0.24 | 0.68 | -11.05 | -5.79 | -6.51 | 2.24 | -0.83 |
Endosome | -5.23 | 0.95 | -3.37 | 3.30 | -4.33 | -4.56 | 3.20 | -5.17 | -1.25 | -6.49 | -6.73 | 3.80 | -5.70 | 0.09 | -7.13 |
Golgi | -1.70 | 1.69 | 1.01 | 3.67 | -3.25 | -2.46 | 3.21 | -3.38 | -2.48 | -3.85 | -3.25 | 2.82 | -3.10 | -1.73 | -3.85 |
Lipid Particles | -0.56 | 3.35 | 2.91 | 4.82 | -1.70 | 0.88 | 4.71 | 5.88 | 4.96 | 2.83 | 0.51 | 6.64 | 6.60 | 6.74 | -0.30 |
Mitochondria | -4.50 | 1.65 | -0.11 | 5.23 | -3.78 | -5.29 | 4.81 | -3.40 | 2.43 | -7.80 | -6.98 | 4.23 | -3.30 | 4.08 | -7.64 |
Mitotic Spindle | -5.07 | -3.52 | -6.09 | -4.69 | -5.34 | -2.76 | -1.15 | -13.97 | -13.02 | -9.76 | -5.49 | -4.46 | -15.21 | -13.27 | -12.60 |
None | -7.42 | 3.24 | -1.26 | 6.06 | -2.69 | 5.01 | 1.69 | 4.45 | -0.24 | 1.00 | 0.09 | 5.73 | 4.03 | 4.63 | -1.82 |
Nuclear Periphery | 1.39 | 3.37 | 0.30 | -0.94 | -2.76 | -4.97 | 3.76 | -4.25 | -0.30 | -6.56 | -2.99 | 4.05 | -3.08 | -0.80 | -6.40 |
Nuclear Periphery Foci | -4.54 | 1.36 | -0.81 | 3.35 | -1.94 | 1.32 | 6.73 | 4.95 | 4.36 | -2.44 | -0.95 | 5.69 | 4.43 | 6.04 | -1.42 |
Nucleolus | 1.53 | -3.69 | 0.00 | -1.17 | 2.84 | 11.57 | 10.00 | 12.70 | 3.02 | -2.27 | 11.60 | 8.64 | 10.73 | 1.20 | 1.86 |
Nucleus | 6.54 | 4.20 | 8.00 | 3.51 | 5.65 | -9.90 | -10.95 | 3.91 | 14.35 | 13.18 | -5.67 | -9.02 | 7.81 | 14.52 | 16.33 |
Peroxisomes | -1.75 | 1.39 | 0.52 | 3.46 | -3.96 | -1.78 | -1.37 | -3.05 | -1.20 | -1.29 | -2.77 | 2.11 | -1.81 | 1.40 | -5.66 |
Vacuole | -4.30 | -1.33 | -4.27 | -2.02 | -3.93 | -5.19 | -3.35 | -9.61 | -7.85 | -5.16 | -6.97 | -4.52 | -10.32 | -7.83 | -8.65 |
Vacuole Periphery | -6.64 | -1.69 | -4.95 | -0.34 | -4.59 | -4.82 | -0.14 | -7.27 | -4.54 | -7.25 | -7.36 | -1.94 | -7.98 | -4.35 | -7.66 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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