Standard name
Human Ortholog
Description NADP+ dependent arabinose dehydrogenase; involved in carbohydrate metabolism; purified as homodimer; naturally occurs with a N-terminus degradation product
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0.05 0.05 0.1
Bud Neck 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 – 0 0 0 0 0 0 0 0 0.05 0 0 0 0.06 0 0 0 0 0 0 0 0
Cytoplasm 0.94 0.96 – 1.0 0.94 0.84 0.83 0.75 0.77 0.7 0.75 0.8 0.99 0.96 0.97 0.97 0.97 0.97 0.87 0.93 0.87 0.87 0.87 0.78
Endoplasmic Reticulum 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0
Endosome 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.08 0 – 0 0 0.1 0.07 0.07 0.17 0.1 0.18 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 – 0 0.05 0.09 0.1 0.14 0.1 0.18 0.09 0.11 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 – 0 1 3 1 4 3 5 2 5 0 1 1 0 0 0 1 0 0 0 0 0
Bud 1 0 – 0 2 1 5 2 4 8 6 6 0 2 2 0 0 0 4 3 0 8 9 23
Bud Neck 0 0 – 0 2 1 0 1 1 1 1 1 0 0 0 0 0 0 1 0 0 1 0 1
Bud Site 0 0 – 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 – 1 6 1 6 4 7 8 5 14 1 0 0 15 4 10 0 0 0 0 1 1
Cytoplasm 232 72 – 143 314 246 319 244 236 247 168 215 123 175 175 258 145 250 217 77 32 150 168 174
Endoplasmic Reticulum 3 1 – 0 2 3 7 10 1 11 5 4 0 1 0 3 5 15 0 0 0 0 1 2
Endosome 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0 0 1
Golgi 2 0 – 0 1 0 0 0 0 0 0 1 0 0 0 1 0 0 3 0 0 0 0 2
Mitochondria 20 1 – 2 3 29 26 23 52 35 40 12 0 0 0 0 0 1 8 0 0 2 2 4
Nucleus 1 0 – 0 1 3 6 8 10 4 5 3 0 1 1 1 0 1 0 0 0 1 0 0
Nuclear Periphery 0 1 – 0 0 2 4 1 4 3 1 1 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 – 0 0 0 2 1 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 – 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 – 0 1 0 1 2 1 1 0 1 0 0 0 0 0 0 3 0 0 1 3 4
Vac/Vac Membrane 0 0 – 0 16 25 39 46 32 63 20 30 0 5 5 4 2 2 1 0 1 4 2 2
Unique Cell Count 248 75 143 335 292 386 326 307 351 224 269 124 182 181 266 149 259 249 83 38 174 195 224
Labelled Cell Count 259 75 146 349 314 416 347 351 386 255 293 124 185 184 282 156 279 249 83 38 174 195 224


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.5 7.2 – 5.0 7.8 7.8 9.3 11.4 10.8 12.1 12.4 14.9 6.1 9.2 9.7 28.6 30.5 30.4 10.2 7.7 10.9
Std Deviation (1e-4) 1.6 2.9 – 1.3 3.5 4.4 5.2 6.0 5.9 6.5 6.0 7.5 2.0 5.7 5.6 6.9 8.3 9.8 12.1 6.4 10.5
Intensity Change (Log2) – – – – – – – – – – – – – – – – – – – – –


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – –
Bud Neck – – – – – – – – – – – – – – –
Bud Site – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – –
Endosome – – – – – – – – – – – – – – –
Golgi – – – – – – – – – – – – – – –
Mitochondria – – – – – – – – – – – – – – –
Nuclear Periphery – – – – – – – – – – – – – – –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – –
Peroxisomes – – – – – – – – – – – – – – –
SpindlePole – – – – – – – – – – – – – – –
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – – –
Cytoplasm – – – – – – – – – – – – – – –
Nucleus – – – – – – – – – – – – – – –
Vacuole – – – – – – – – – – – – – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 5.4032 6.2533 6.5849 8.0732 6.1293 6.2026 7.6485 9.9062 8.2804 7.6818 8.0667 7.7651 5.308 7.0352 4.0324 3.8824 3.103 6.8438
Actin 0.0331 0.0057 0.0022 0.004 0.0007 0.0019 0.0269 0.0018 0.0111 0.0086 0.0339 0.0073 0.0293 0.002 0.0532 0.0053 0.0045 0.0081
Bud 0.002 0.0028 0.0017 0.0007 0.0007 0.0007 0.0008 0.0107 0.0021 0.0025 0.0005 0.0015 0.0004 0.0067 0.0008 0.0004 0.0002 0.0004
Bud Neck 0.0038 0.0005 0.0009 0.002 0.0005 0.0011 0.0025 0.0004 0.0005 0.001 0.0009 0.0018 0.0016 0.0002 0.0004 0.0004 0.0004 0.0007
Bud Periphery 0.0027 0.0014 0.0019 0.0004 0.0003 0.0004 0.0011 0.0036 0.0016 0.0011 0.0004 0.002 0.0004 0.0022 0.0004 0.0009 0.0002 0.0006
Bud Site 0.0052 0.015 0.0016 0.0007 0.0002 0.0003 0.0033 0.0075 0.0015 0.0005 0.0011 0.0005 0.0018 0.0036 0.0047 0.0002 0.0004 0.0004
Cell Periphery 0.0004 0.0003 0.0002 0 0 0 0.0004 0.0002 0.0001 0.0001 0.0002 0.0001 0.0002 0.0001 0.0001 0.0002 0.0001 0.0001
Cytoplasm 0.72 0.8626 0.838 0.7566 0.9502 0.8539 0.7601 0.9028 0.8514 0.8818 0.7739 0.8848 0.7408 0.905 0.7713 0.7315 0.7841 0.9259
Cytoplasmic Foci 0.0374 0.0079 0.0165 0.0476 0.0076 0.0242 0.023 0.0144 0.0153 0.0264 0.0442 0.0075 0.0128 0.0087 0.0236 0.0042 0.0124 0.0025
Eisosomes 0.0003 0 0.0001 0 0 0 0.0003 0 0 0 0.0003 0 0.0008 0 0.0003 0.0002 0.0001 0.0001
Endoplasmic Reticulum 0.0045 0.0015 0.0025 0.0009 0.0035 0.0022 0.0069 0.0013 0.002 0.0039 0.0074 0.0038 0.0214 0.0015 0.002 0.0036 0.0022 0.0023
Endosome 0.0427 0.0016 0.0148 0.042 0.0035 0.0095 0.0297 0.0023 0.0091 0.0153 0.015 0.013 0.0252 0.0055 0.0139 0.0545 0.0607 0.0082
Golgi 0.0135 0.001 0.0035 0.0145 0.0002 0.0046 0.0073 0.0001 0.0039 0.0033 0.0034 0.0031 0.0068 0.001 0.0064 0.0287 0.0595 0.0041
Lipid Particles 0.0083 0.0001 0.0016 0.0037 0.0001 0.0007 0.0169 0.0001 0.0019 0.0082 0.0099 0.0047 0.0107 0.0046 0.0048 0.0062 0.0405 0.0011
Mitochondria 0.0171 0.0004 0.0048 0.0013 0.0002 0.0091 0.0026 0.0003 0.001 0.0004 0.0023 0.0022 0.0054 0.0011 0.0039 0.0464 0.0186 0.0189
None 0.0763 0.0916 0.0815 0.0918 0.0202 0.075 0.0624 0.0439 0.0676 0.0159 0.0794 0.0278 0.0796 0.0512 0.0926 0.0943 0.0049 0.0142
Nuclear Periphery 0.0056 0.001 0.0023 0.0013 0.0016 0.0013 0.0099 0.0014 0.0022 0.005 0.004 0.012 0.0326 0.0008 0.0031 0.0019 0.0017 0.0025
Nucleolus 0.0005 0.0001 0.0008 0.0001 0 0 0.0005 0.0001 0.0001 0.0001 0.0004 0.0009 0.0005 0.0001 0.0006 0.0001 0.0001 0.0001
Nucleus 0.0066 0.0038 0.0074 0.0192 0.0081 0.0076 0.0276 0.0065 0.0201 0.0127 0.0075 0.0183 0.0179 0.0024 0.0065 0.0105 0.0019 0.0073
Peroxisomes 0.01 0.0008 0.0114 0.0103 0.0001 0.0049 0.0077 0.0002 0.0052 0.0039 0.007 0.0019 0.0041 0.0013 0.0018 0.0002 0.002 0.0003
Punctate Nuclear 0.0061 0.0013 0.0036 0.0014 0.001 0.0012 0.0068 0.0013 0.0018 0.0079 0.0043 0.0044 0.0045 0.0006 0.0082 0.0003 0.0008 0.0004
Vacuole 0.0032 0.0007 0.0023 0.0011 0.001 0.0009 0.0026 0.001 0.0013 0.001 0.0031 0.0015 0.0017 0.0012 0.0009 0.0054 0.002 0.0011
Vacuole Periphery 0.001 0.0001 0.0005 0.0003 0.0002 0.0004 0.0008 0.0001 0.0002 0.0003 0.0011 0.0008 0.0015 0.0002 0.0005 0.0047 0.0029 0.0008

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 149.2528 90.5369 70.011 124.7041 76.714 152.3667 115.8727 137.5519 141.3953 130.0777
Translational Efficiency 1.7301 1.8952 2.2565 1.5947 2.1222 2.0251 1.728 1.6018 1.8288 1.5992

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1763 165 2851 1186 1251 1441 220 1272 3014 1606 3071 2458

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 702.64 910.65 898.98 1094.53 691.00 758.68 1277.94 1207.01 697.81 774.29 926.13 1152.74
Standard Deviation 106.40 162.03 272.78 484.88 139.65 116.05 356.90 572.94 121.45 130.04 296.23 535.23
Intensity Change Log 2 — 0.374111 0.355503 0.639454 — 0.134806 0.887062 0.804680 — 0.260411 0.643430 0.723742

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000221 0.002032 0.000170 0.000660 0.000103 0.000145 0.000609 0.000599 0.000172 0.000339 0.000202 0.000628
Bud Neck 0.016542 0.018920 0.010201 0.003786 0.006126 0.022129 0.002426 0.004829 0.012219 0.021799 0.009644 0.004325
Bud Site 0.009540 0.032879 0.022050 0.029473 0.006493 0.010794 0.006801 0.027707 0.008275 0.013063 0.020958 0.028559
Cell Periphery 0.000159 0.000202 0.000113 0.000263 0.000168 0.000145 0.000244 0.000171 0.000163 0.000151 0.000122 0.000215
Cytoplasm 0.512155* 0.362954* 0.701006* 0.600127* 0.615293* 0.528074* 0.532855* 0.601297* 0.554963* 0.511109* 0.688960* 0.600733*
Cytoplasmic Foci 0.241285* 0.288303* 0.009457 0.026173 0.177559 0.186356* 0.026852 0.025592 0.214835* 0.196830* 0.010703 0.025873
Eisosomes 0.000151 0.000157 0.000015 0.000036 0.000091 0.000104 0.000057 0.000026 0.000126 0.000110 0.000018 0.000031
Endoplasmic Reticulum 0.001135 0.005365 0.000644 0.001537 0.001672 0.000498 0.002822 0.002857 0.001358 0.000998 0.000800 0.002220
Endosome 0.014011 0.030119 0.001270 0.011478 0.009521 0.016714 0.005728 0.014892 0.012147 0.018092 0.001589 0.013245
Golgi 0.004079 0.012775 0.000439 0.012731 0.002035 0.002873 0.000330 0.014383 0.003230 0.003890 0.000431 0.013586
Lipid Particles 0.008018 0.003868 0.000501 0.001241 0.006527 0.004657 0.002546 0.001067 0.007399 0.004576 0.000648 0.001151
Mitochondria 0.005399 0.002312 0.001483 0.009692 0.002669 0.006508 0.000198 0.003732 0.004266 0.006077 0.001391 0.006608
Mitotic Spindle 0.004355 0.016966 0.020746 0.034199 0.000912 0.002225 0.012458 0.067658 0.002926 0.003740 0.020152 0.051514
None 0.005510 0.001254 0.006343 0.005066 0.010082 0.005670 0.005850 0.008617 0.007408 0.005216 0.006307 0.006904
Nuclear Periphery 0.000337 0.000513 0.000806 0.000963 0.000450 0.000368 0.001269 0.000786 0.000384 0.000383 0.000839 0.000871
Nuclear Periphery Foci 0.001103 0.001688 0.000463 0.002657 0.001550 0.000436 0.006731 0.002016 0.001289 0.000565 0.000913 0.002325
Nucleolus 0.001353 0.000737 0.000447 0.000327 0.001718 0.001721 0.000277 0.000417 0.001504 0.001620 0.000435 0.000373
Nucleus 0.110998 0.061040 0.175769* 0.128159 0.110181 0.127160 0.216758* 0.105049 0.110659 0.120367 0.178705* 0.116199
Peroxisomes 0.007001 0.023219 0.000551 0.001319 0.002237 0.005165 0.000285 0.001476 0.005024 0.007020 0.000532 0.001401
Vacuole 0.055432 0.133021 0.045748 0.123947 0.043692 0.076100 0.174562 0.108691 0.050559 0.081948 0.054976 0.116052
Vacuole Periphery 0.001215 0.001676 0.001777 0.006164 0.000920 0.002156 0.000342 0.008138 0.001093 0.002106 0.001674 0.007185

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -3.08 0.99 -3.93 2.61 -5.45 -2.26 -3.70 -4.12 -3.43 1.76 -2.40 -1.21 -5.24 -1.43 -4.45
Bud Neck -0.27 5.48 11.02 3.91 7.38 -8.66 4.05 2.84 9.97 -1.24 -5.30 3.65 10.98 10.94 6.73
Bud Site -4.28 -3.52 -2.81 3.07 0.29 -2.58 -0.86 -4.16 -1.99 -3.98 -3.21 -4.56 -4.71 -1.25 0.22
Cell Periphery -1.17 3.55 -2.09 -0.56 -4.65 1.78 -3.61 0.86 -0.94 3.99 1.04 4.16 -1.44 -2.30 -4.97
Cytoplasm 6.60 -14.50 -1.16 -6.98 11.25 8.35 7.58 5.00 -3.10 -4.66 5.14 -9.38 1.55 -3.63 10.33
Cytoplasmic Foci -2.03 43.85 41.60 15.21 -6.46 -1.10 25.43 30.09 32.19 2.50 3.16 53.10 50.86 35.31 -6.94
Eisosomes -0.07 16.21 13.90 6.05 -11.44 -1.71 5.54 15.32 14.57 5.66 2.45 20.46 18.55 15.01 -8.26
Endoplasmic Reticulum -1.17 0.54 -0.97 1.07 -4.74 3.25 -3.22 0.81 -1.63 3.70 0.92 0.95 0.05 -0.90 -0.94
Endosome -3.59 15.94 9.56 6.11 -3.83 -5.38 5.73 4.66 8.49 -0.13 -4.59 19.63 10.37 10.94 -5.65
Golgi -1.49 9.41 0.53 1.59 -2.52 -1.43 4.59 -1.58 -0.90 -3.12 -0.71 10.21 -0.51 0.04 -4.00
Lipid Particles 5.08 12.00 10.54 3.96 -9.18 2.33 4.90 7.34 7.15 4.80 4.73 14.34 12.80 7.77 -6.69
Mitochondria 3.13 4.53 1.76 -0.56 -1.35 -3.19 3.62 1.88 5.06 -2.94 -1.64 5.04 2.71 3.82 -1.38
Mitotic Spindle -2.16 -6.50 -5.94 -0.91 -1.47 -1.60 -2.20 -10.83 -10.40 -7.47 -0.70 -8.19 -11.95 -11.47 -5.70
None 4.08 -0.35 0.74 -6.84 2.72 2.59 3.80 2.10 -1.21 -3.68 1.90 1.93 1.77 -0.78 -0.34
Nuclear Periphery -1.84 -17.53 -9.77 -5.54 0.69 1.06 -9.06 -7.66 -12.48 5.05 -0.26 -18.96 -12.90 -14.05 3.34
Nuclear Periphery Foci -0.99 0.87 -2.41 -1.55 -7.97 4.63 -8.01 -0.19 -7.27 8.16 2.28 0.34 -2.11 -9.92 -5.36
Nucleolus 3.00 5.05 6.13 4.24 4.69 -0.08 3.26 3.16 5.89 -1.20 -0.38 5.12 5.83 6.25 3.73
Nucleus 5.74 -21.24 -7.35 -9.86 10.92 -3.04 -7.83 -3.58 -0.59 6.17 -2.22 -24.03 -7.65 -4.14 15.68
Peroxisomes -3.26 10.72 10.70 4.76 -0.47 -5.29 8.51 6.30 8.90 -2.62 -2.43 11.91 11.97 9.65 -0.97
Vacuole -6.40 -8.43 -23.48 -5.10 -19.38 -9.26 -12.46 -22.16 -16.65 2.35 -9.96 -14.01 -31.96 -22.40 -21.44
Vacuole Periphery -0.84 0.30 -0.83 -0.17 -0.87 -4.18 5.01 -1.80 -0.17 -2.73 -3.89 0.17 -1.89 0.18 -1.61
Standard name
Human Ortholog
Description NADP+ dependent arabinose dehydrogenase; involved in carbohydrate metabolism; purified as homodimer; naturally occurs with a N-terminus degradation product
Localization
Cell Percentages cytoplasm (59%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

Ara1

Ara1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Ara1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available