Standard name
Human Ortholog
Description Modulator of cell separation and mitotic exit; inhibits separation through Ub-dependent Ace2p proteolysis; part of a daughter-specific switch induced by the mitotic exit network that inhibits exit and resets the cell cycle after the execution of MEN function, blocking Tem1p and Cdc15 association; required for chromosome stability and multiple mitotic checkpoints; regulated by SCF; haploid transcription regulated by Ste12p; contains 12 degenerate leucine-rich repeat motifs and an atypical F-box
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Cytoplasm 0.67 0.81 0.94 0.97 0.88 0.73 0.68 0.67 0.58 0.57 0.38 0.28 0.97 0.99 0.99 – 0.95 0.95 0.46 0.56 0.56 0.8 0.73 0.65
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0.09 0.08 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Mitochondria 0.16 0 0 0.13 0.14 0.45 0.46 0.4 0.64 0.65 0.75 0.84 0 0 0 – 0 0 0.07 0.06 0.2 0.05 0 0.06
Nucleus 0.11 0.09 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0.16 0.11 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0.13 0.07 0 0 0 0
Vac/Vac Membrane 0.13 0.13 0.05 0 0 0 0 0 0 0 0 0 0 0 0 – 0.06 0 0.07 0.09 0.05 0 0.05 0.09
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 1 5 12 0 3 8
Bud 3 1 3 0 2 10 7 9 10 11 6 4 0 2 0 – 0 0 6 20 8 2 5 10
Bud Neck 0 1 1 1 3 3 4 8 1 3 0 2 0 0 0 – 2 3 0 2 6 0 1 4
Bud Site 0 0 0 0 0 2 0 0 5 2 2 2 0 0 0 – 0 0 – – – – – –
Cell Periphery 2 1 1 2 1 0 0 2 0 0 0 2 1 0 1 – 11 6 0 2 2 1 1 3
Cytoplasm 102 366 395 222 263 310 351 298 240 279 78 74 266 338 364 – 525 507 84 260 251 136 221 217
Endoplasmic Reticulum 0 0 1 0 2 0 0 1 0 0 5 6 2 0 4 – 49 42 1 1 4 1 3 5
Endosome 3 3 1 0 3 2 0 0 1 1 0 0 0 3 0 – 6 5 1 4 14 0 3 4
Golgi 0 0 1 0 1 1 0 0 0 0 0 0 0 0 0 – 1 0 0 1 1 1 3 0
Mitochondria 24 11 13 29 43 190 235 179 265 317 152 221 0 2 0 – 10 10 13 28 89 8 13 21
Nucleus 17 39 3 0 2 2 3 2 3 2 1 1 4 0 0 – 1 2 29 51 5 2 10 6
Nuclear Periphery 0 0 0 0 0 0 0 0 0 1 0 1 0 0 0 – 0 0 0 0 0 0 0 0
Nucleolus 1 0 0 0 1 1 0 1 0 2 1 1 1 0 0 – 0 1 0 0 0 0 0 0
Peroxisomes 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 – 1 2 0 1 1 0 0 0
SpindlePole 1 0 2 0 1 5 8 13 7 8 2 5 0 0 0 – 4 1 22 32 12 4 12 10
Vac/Vac Membrane 20 60 19 1 3 5 8 6 5 5 1 3 5 0 1 – 35 22 13 43 20 6 14 28
Unique Cell Count 153 450 419 230 298 426 514 446 417 491 204 264 274 342 369 553 534 182 465 446 171 304 333
Labelled Cell Count 173 482 440 255 326 531 616 519 537 631 248 322 279 345 370 645 601 182 465 446 171 304 333


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 3.9 4.3 4.0 3.5 3.8 3.2 2.6 3.0 2.9 2.5 2.7 2.5 4.5 4.2 4.4 4.8 5.9 5.4 4.3 4.5 5.0
Std Deviation (1e-4) 1.4 0.8 1.6 1.5 1.8 1.4 0.7 1.0 1.1 0.7 0.6 0.7 1.4 1.2 1.6 1.9 1.9 2.0 1.9 1.9 1.5
Intensity Change (Log2) – – – -0.18 -0.07 -0.31 -0.63 -0.43 -0.47 -0.66 -0.53 -0.65 0.16 0.07 0.14 0.28 0.57 0.45 0.1 0.17 0.32

WT3RAP60RAP140RAP220RAP300RAP380RAP460RAP540RAP620RAP7000246WT3HU80HU120HU1600246WT3rpd3Δ_1rpd3Δ_2rpd3Δ_30246WT1AF100AF140AF1800246
rapamycinhydroxy-urearpd3 knockoutalpha-factorMultiple Condition Time-Course Intensity PlotsGFP Intensity (1e-4)GFP Intensity (1e-4)GFP Intensity (1e-4)GFP Intensity (1e-4)

Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – 0 0
Bud Neck – – – – – – – – – – – – – 0 0
Bud Site – – – – – – – – – – – – – 0 0
Cell Periphery – – – – – – – – – – – – – 0 0
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – 0 0
Endosome – – – – – – – – – – – – – 0 0
Golgi – – – – – – – – – – – – – 0 0
Mitochondria – – – – – – – – – – – – – 0 0
Nuclear Periphery – – – – – – – – – – – – – 0 0
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – 0 0
Peroxisomes – – – – – – – – – – – – – 0 0
SpindlePole – – – – – – – – – – – – – 0 0
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – 0 0
Cytoplasm – – – – – – – – – – – – – 0 0
Nucleus – – – – – – – – – – – – – 0 0
Vacuole – – – – – – – – – – – – – 1.2 -0.3

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 3.1693 1.2017 1.3169 1.1106 0.343 1.8065 2.9311 2.3238 2.4789 2.6638 2.0554 2.406 4.2038 2.9067 3.1727 2.9359 3.3377 3.1537
Actin 0.1846 0.0009 0.0001 0.0011 0.0041 0.0016 0.0059 0.0029 0.0287 0.0008 0.003 0.0052 0.0109 0.0005 0.0146 0.0004 0.003 0.0019
Bud 0.007 0.0004 0 0.0062 0.0018 0.0058 0.0004 0.017 0.0156 0.0002 0.0002 0.0018 0.0005 0.0066 0.0036 0.0004 0.007 0.0015
Bud Neck 0.0043 0.0001 0 0.0001 0.0002 0.0067 0.0008 0.0008 0.0006 0 0.0001 0.0015 0.0088 0.0005 0.0026 0.0005 0.0001 0.0039
Bud Periphery 0.0344 0.0009 0.0001 0.0044 0.0038 0.0072 0.0005 0.0147 0.0044 0.0007 0.0003 0.001 0.0008 0.0079 0.0087 0.0004 0.0104 0.0019
Bud Site 0.0184 0.0013 0.0001 0.0019 0.001 0.0093 0.0027 0.0263 0.0112 0 0.0006 0.0003 0.0067 0.0151 0.0092 0.0007 0.0009 0.0006
Cell Periphery 0.0052 0.0003 0.0002 0.0001 0.0001 0.0003 0.0002 0.001 0.0004 0.0001 0.0001 0.0001 0.0005 0.0004 0.0007 0.0001 0.0003 0.0003
Cytoplasm 0.1914 0.0986 0.0315 0.0896 0.017 0.1474 0.2142 0.1554 0.1082 0.0399 0.1542 0.2222 0.1769 0.0819 0.0854 0.0933 0.0597 0.2033
Cytoplasmic Foci 0.0198 0.0024 0.0018 0.0049 0.0083 0.0101 0.0607 0.0051 0.0171 0.0036 0.0049 0.0137 0.0326 0.0069 0.0106 0.0425 0.0075 0.0136
Eisosomes 0.0019 0.0001 0.0002 0.0004 0.0004 0.0001 0.0004 0.0003 0.0003 0.0002 0.0001 0.0001 0.0004 0.0002 0.0003 0.0002 0.0001 0.0001
Endoplasmic Reticulum 0.0216 0.0014 0.0004 0.0011 0.0003 0.002 0.0043 0.0038 0.0019 0.0006 0.0009 0.0021 0.0102 0.0023 0.0044 0.0013 0.001 0.0022
Endosome 0.0236 0.0005 0.0002 0.0005 0.0002 0.0015 0.0296 0.0077 0.0035 0.0004 0.0179 0.0051 0.0223 0.0015 0.0142 0.0161 0.0018 0.0027
Golgi 0.011 0.0001 0 0.0002 0.0002 0.0002 0.0158 0.0022 0.0029 0.0001 0.0224 0.004 0.0036 0.0001 0.0075 0.0016 0.0047 0.0003
Lipid Particles 0.0131 0.0002 0.0002 0.0003 0.0007 0.0004 0.0171 0.0005 0.0084 0.0003 0.0009 0.0026 0.0156 0.0003 0.0041 0.0019 0.0097 0.0008
Mitochondria 0.0299 0.0002 0.0002 0.0009 0.001 0.0011 0.0083 0.0036 0.0129 0.0004 0.0138 0.0022 0.0018 0.0007 0.0043 0.0005 0.0289 0.0009
None 0.3011 0.8906 0.9638 0.8622 0.9449 0.5756 0.4482 0.7307 0.7491 0.9475 0.778 0.6439 0.5325 0.8468 0.795 0.8285 0.8305 0.6766
Nuclear Periphery 0.0171 0.0004 0.0002 0.0024 0.0007 0.0039 0.0091 0.0027 0.0009 0.0007 0.0003 0.0067 0.0097 0.002 0.0083 0.0006 0.0002 0.003
Nucleolus 0.007 0.0001 0.0001 0.001 0.0012 0.003 0.0017 0.0009 0.0007 0.0003 0.0001 0.0013 0.0014 0.0009 0.0006 0.0004 0.0001 0.0011
Nucleus 0.0117 0.0005 0.0002 0.0058 0.0016 0.199 0.1373 0.0162 0.0072 0.0005 0.0003 0.0642 0.1401 0.0169 0.0118 0.0009 0.0003 0.0714
Peroxisomes 0.0107 0.0001 0.0001 0.0005 0.0014 0.0002 0.03 0.0004 0.0227 0.0002 0.0005 0.0031 0.0099 0.0004 0.0043 0.0017 0.033 0.0008
Punctate Nuclear 0.0298 0.0003 0.0003 0.0157 0.0106 0.0213 0.0095 0.0037 0.0015 0.0027 0.0003 0.0173 0.012 0.0053 0.0019 0.0054 0.0003 0.011
Vacuole 0.0448 0.0005 0.0002 0.0005 0.0002 0.0029 0.0027 0.003 0.0013 0.0006 0.0007 0.0012 0.0023 0.0026 0.0056 0.0022 0.0003 0.0019
Vacuole Periphery 0.0115 0.0001 0 0.0002 0.0001 0.0004 0.0007 0.001 0.0004 0.0003 0.0005 0.0005 0.0007 0.0003 0.0023 0.0002 0.0001 0.0004

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 15.7152 14.3473 39.9237 153.9837 119.6512 15.3723 15.111 16.9301 85.2862 155.6867
Translational Efficiency 0.1324 0.1742 0.2881 0.2099 0.3775 0.1662 0.1473 0.1551 0.2286 0.3435

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✔
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1186 1454 282 1660 1183 2062 245 3050 2369 3516 527 4710

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 629.18 688.34 867.28 884.58 623.88 720.09 930.68 987.58 626.53 706.96 896.75 951.28
Standard Deviation 65.48 85.65 119.44 153.76 72.74 104.81 119.42 394.01 69.25 98.59 123.54 333.59
Intensity Change Log 2 — 0.129649 0.463025 0.491520 — 0.206909 0.577017 0.662629 — 0.168632 0.520905 0.579248

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000048 0.000380 0.000385 0.000833 0.000168 0.000356 0.000643 0.000782 0.000108 0.000366 0.000505 0.000800
Bud Neck 0.010311 0.027912 0.003054 0.022081 0.010718 0.037548 0.008183 0.023920 0.010514 0.033563 0.005438 0.023272
Bud Site 0.002686 0.014951 0.001869 0.034174 0.003397 0.017101 0.010523 0.036993 0.003041 0.016212 0.005892 0.035999
Cell Periphery 0.000161 0.000186 0.000176 0.000342 0.000166 0.000219 0.000275 0.000559 0.000163 0.000205 0.000222 0.000483
Cytoplasm 0.537447 0.396285 0.120134 0.371780 0.440113 0.441059 0.188299 0.516101 0.488842 0.422543 0.151824 0.465236
Cytoplasmic Foci 0.181026 0.170640 0.013129 0.012078 0.180131 0.145552 0.008873 0.003951 0.180579 0.155927 0.011150 0.006816
Eisosomes 0.000158 0.000103 0.000071 0.000036 0.000139 0.000085 0.000082 0.000017 0.000149 0.000092 0.000076 0.000024
Endoplasmic Reticulum 0.000399 0.001177 0.004823 0.003296 0.000675 0.000955 0.007255 0.000816 0.000537 0.001046 0.005954 0.001690
Endosome 0.006595 0.014518 0.002681 0.005269 0.007606 0.015131 0.003318 0.002037 0.007100 0.014877 0.002977 0.003176
Golgi 0.001171 0.004267 0.000072 0.002050 0.001683 0.003592 0.000115 0.001449 0.001427 0.003871 0.000092 0.001661
Lipid Particles 0.007350 0.006376 0.002329 0.001022 0.010390 0.005475 0.001986 0.000328 0.008868 0.005847 0.002170 0.000572
Mitochondria 0.002974 0.012447 0.001184 0.004023 0.005066 0.015640 0.000959 0.006098 0.004018 0.014319 0.001079 0.005366
Mitotic Spindle 0.000768 0.005655 0.005629 0.076023 0.000867 0.013787 0.019566 0.062135 0.000818 0.010424 0.012108 0.067030
None 0.005108 0.008990 0.005502 0.010452 0.005983 0.006960 0.006801 0.014377 0.005545 0.007799 0.006106 0.012994
Nuclear Periphery 0.000374 0.000636 0.001728 0.002000 0.000765 0.000888 0.002051 0.001231 0.000569 0.000784 0.001878 0.001502
Nuclear Periphery Foci 0.000214 0.000445 0.000744 0.002082 0.000314 0.000479 0.001655 0.000483 0.000264 0.000465 0.001168 0.001047
Nucleolus 0.000996 0.004505 0.001376 0.001632 0.002302 0.003809 0.001513 0.001266 0.001648 0.004096 0.001439 0.001395
Nucleus 0.204044 0.253333 0.781231 0.387264 0.290911 0.225629 0.628145 0.293512 0.247422 0.237085 0.710062 0.326554
Peroxisomes 0.002130 0.012705 0.000324 0.001037 0.004721 0.010839 0.000299 0.000384 0.003424 0.011611 0.000312 0.000614
Vacuole 0.035534 0.062576 0.053255 0.058380 0.032813 0.052943 0.109165 0.031892 0.034175 0.056927 0.079247 0.041228
Vacuole Periphery 0.000508 0.001913 0.000306 0.004144 0.001071 0.001954 0.000295 0.001668 0.000789 0.001937 0.000301 0.002540

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -6.52 -9.38 -11.28 -4.60 -3.36 -1.86 -4.80 -2.85 -1.24 1.35 -4.38 -8.25 -6.53 -2.79 -0.24
Bud Neck -8.15 5.60 -6.51 2.70 -13.42 -11.55 2.79 -9.67 5.31 -11.67 -14.17 5.68 -11.93 5.75 -18.87
Bud Site -6.94 0.69 -9.83 -2.93 -10.57 -7.74 -1.53 -11.15 -3.34 -6.14 -10.63 -1.18 -15.26 -4.51 -11.84
Cell Periphery -1.56 -0.54 -3.21 -1.81 -1.83 -1.02 -1.80 -2.46 -0.90 -1.16 -1.35 -1.67 -3.23 -1.33 -1.95
Cytoplasm 14.01 35.95 27.32 12.16 -14.71 -0.18 18.63 4.52 5.18 -16.93 9.27 37.32 19.80 10.42 -24.60
Cytoplasmic Foci 1.24 30.41 33.04 29.48 0.66 5.03 29.74 31.25 34.19 2.80 4.86 42.32 45.75 45.22 2.72
Eisosomes 5.66 6.06 12.15 9.20 4.55 7.63 3.10 17.55 17.11 9.96 9.51 6.62 21.30 19.10 11.17
Endoplasmic Reticulum -8.07 -10.40 -21.83 -17.11 1.98 -2.44 -8.37 -7.17 -4.30 7.58 -6.73 -12.73 -21.71 -15.30 8.57
Endosome -6.93 5.21 4.17 10.24 -1.51 -6.66 4.92 9.54 14.33 5.32 -9.65 7.22 10.58 17.84 3.70
Golgi -3.83 3.06 1.09 4.72 -3.20 -2.73 3.17 2.03 5.91 -2.93 -4.75 4.36 2.31 7.49 -4.24
Lipid Particles 0.95 5.63 7.47 8.77 3.26 5.30 9.72 12.74 9.44 6.97 4.56 10.85 14.69 12.98 7.87
Mitochondria -5.67 2.02 0.59 6.90 -2.64 -6.12 4.38 1.43 8.04 -5.37 -8.47 4.51 1.20 10.40 -5.55
Mitotic Spindle -3.65 -0.96 -14.54 -13.20 -11.38 -7.16 -2.26 -19.50 -13.49 -9.22 -8.00 -2.34 -24.28 -19.03 -15.08
None -2.92 0.60 -4.27 -0.13 -5.74 -0.83 1.56 -8.85 -9.88 -12.46 -2.65 1.54 -10.47 -7.59 -14.18
Nuclear Periphery -3.75 -11.73 -27.03 -23.96 -10.58 -0.45 -10.37 -14.46 -5.59 0.77 -1.29 -15.16 -27.06 -12.11 -5.10
Nuclear Periphery Foci -5.22 -4.74 -7.48 -7.12 -6.31 -1.55 -6.02 -3.24 -1.71 4.58 -3.28 -7.34 -8.13 -7.20 -2.26
Nucleolus -7.12 -1.65 -5.19 1.54 -3.95 -2.37 2.31 -3.07 -1.17 -6.52 -6.35 1.37 -5.74 -0.04 -7.60
Nucleus -5.36 -34.25 -22.83 -16.12 20.08 7.08 -12.81 -11.09 -20.91 8.42 1.68 -30.29 -23.94 -26.13 19.50
Peroxisomes -9.57 7.67 5.61 10.97 -3.32 -6.42 8.29 8.09 14.20 -1.57 -11.72 10.51 9.82 17.78 -3.14
Vacuole -8.59 -4.68 -19.14 -12.63 -5.84 -7.17 -9.71 -10.15 -3.42 7.86 -11.06 -10.33 -20.46 -10.95 4.41
Vacuole Periphery -8.14 2.89 -1.37 1.14 -1.77 -2.01 1.92 0.77 6.39 -3.40 -4.84 2.38 -0.44 4.35 -3.03
Protein Complexes26S Proteasome complex37S mitochondrial small ribosomal subunit54S mitochondrial large ribosomal subunit60S cytosolic large ribosomal subunitAar2-U5 snRNPAnaphase-Promoting Complex variant 1Anaphase-Promoting Complex variant 2Anaphase-Promoting Complex variant 3Anaphase-Promoting Complex variant 4Arp2/3 complexATG1 kinase complexBLOC-1 complexCcr4-Not complexCentral kinetochore CTF19 complexCleavage and polyadenylation specificity factorcomplexCMG helicase complexCMG-Pol epsilon complexCOMPASS complexCOP9 signalosome COPI vesicle coat complexCoQ6 biosynthesis complexCore mediator complexCORVET complexCTF18-RFC complexCURI complex variant 1CURI complex variant 2CURI complex variant 3Cytoplasmic exosome complex, DIS3 variantDASH complexDNA-directed RNA Polymerase I complexDNA-directed RNA polymerase II complexDNA-directed RNA polymerase III complexDNA replication factor C complexEGO complexELG1-RFC complex Elongator holoenzyme complexEndoplasmic Reticulum Membrane ComplexEukaryotic translation initiation factor 2BcomplexEukaryotic translation initiation factor 3core complexExocystExomer complexFAR complexGeneral transcription factor complex TFIIDGeneral transcription factor complex TFIIHGET4-GET5 transmembrane domain recognition complexGID ubiquitin ligase complexGin4 complexGolgi transport complexGSE complexHMC complexHOPS complexINO80 chromatin remodeling complexLSM1-7 complexLsm1-7-Pat1 complexLSM2-7 complexLSM2-8 complexMCM complexMICOS complexMitochondrial electron transport complex IIIMitochondrial inner membrane pre-sequence translocasecomplexMitochondrial outer membrane translocase holocomplexMitochondrial proton-transporting ATP synthase complexMitochondrial pyruvate dehydrogenase complexNineTeen complexNOP8 60s ribosome pre-assembly complexNuA3 histone acetyltransferase complexNuA4 histone acetyltransferase complexNuclear condensin complexNuclear/nucleolar exosome complex, DIS3-RRP6 variantNuclear pore complexNucleolar ribonuclease MRP complexNucleolar ribonuclease P complexOligosaccharyl transferase complex variant 1Oligosaccharyl transferase complex variant 2PAF1 complexPAS complexPhosphatidylinositol 3-kinase complex IPolarisomePrefoldin complexPRP19-associated complexRad17 RFC-like complexRetromer complexRPD3L histone deacetylase complexRPD3S histone deacetylase complexRQC complexRSC complex variant RSC1RSC complex variant RSC2SAGA complexSCF-Met30 ubiquitin ligase complexSCF-Ufo1 ubiquitin ligase complexSEA complexSeptin complexSET3C histone deacetylase complexSignal recognition particleSLIK (SAGA-like) complexSmall ribosomal subunit processome, variant1Small ribosomal subunit processome, variant2Small ribosomal subunit processome, variant3SMC5-SMC6 SUMO ligase complexSm complexSpliceosomal commitment complexSPOTS complexSWI/SNF chromatin remodelling complexSwr1 chromatin remodelling complexTHO complexTranscription factor TFIIIC complexTranslocon complexTRAPPIII protein complexTRAPPII protein complexTRAPPI protein complexTREX complexU1 snRNPU2 snRNPU4 snRNPU4/U6 snRNPU4/U6.U5 tri-snRNP complexU5 snRNPU6 snRNP complexUTP-A complexUTP-B complexUTP-C complex variant 1UTP-C complex variant 2UTP-C complex variant 3Vacuolar proton translocating ATPase complex,Golgi variantVacuolar proton translocating ATPase complex,vacuole variantCellular Components••••••••••••••••actinbud neckcell peripherycytoplasmendosomeERGolgimitochondrionmixednuclear peripherynucleolusnucleusperoxisomespindle polevacuolar membranevacuole
Standard name
Human Ortholog
Description Modulator of cell separation and mitotic exit; inhibits separation through Ub-dependent Ace2p proteolysis; part of a daughter-specific switch induced by the mitotic exit network that inhibits exit and resets the cell cycle after the execution of MEN function, blocking Tem1p and Cdc15 association; required for chromosome stability and multiple mitotic checkpoints; regulated by SCF; haploid transcription regulated by Ste12p; contains 12 degenerate leucine-rich repeat motifs and an atypical F-box
Localization
Cell Percentages cytoplasm (26%), nucleus (7%), mixed (39%)
Cell Cycle Regulation Yes
cytoplasm - S/G2 (2.6e-11)
cytoplasm - MA (8.8e-12)
nucleus - T/G1 (1.6e-14)
Subcompartmental Group N/A

Amn1

Amn1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Amn1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available