Standard name
Human Ortholog
Description Prephenate dehydrogenase involved in tyrosine biosynthesis; expression is dependent on phenylalanine levels
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.99 0.99 0.97 0.97 0.95 0.88 0.77 0.77 0.72 0.72 0.66 0.65 0.99 0.97 1.0 0.95 0.95 0.97 0.96 0.95 0.92 0.93 0.95 0.93
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0.13 0.21 0.16 0.17 0.24 0.32 0.28 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0.05 0.06 0.1 0.12 0.09 0.15 0.13 0 0 0 0.06 0.05 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 1 0 0 0 1 0 0 1 0 0 0 0 0 0 0 0 2 1 2 0 0 1
Bud 0 0 0 0 0 0 3 1 6 6 9 3 0 0 0 0 0 0 0 0 0 2 1 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 4 2 8 2 5 5 9 3 6 8 8 10 1 5 2 0 1 1 0 0 0 0 0 0
Cytoplasm 380 264 381 198 266 248 302 280 332 405 287 292 445 502 477 79 122 114 361 249 359 303 401 416
Endoplasmic Reticulum 1 0 6 1 3 1 14 9 19 18 3 12 2 6 3 3 5 1 1 1 6 1 0 6
Endosome 0 0 1 1 0 0 0 0 0 1 1 0 1 0 0 0 2 0 1 0 2 1 1 0
Golgi 1 0 1 2 0 0 0 2 0 0 0 0 0 0 0 0 1 0 3 0 3 1 0 2
Mitochondria 3 2 0 3 6 36 82 58 79 134 138 126 0 0 0 0 0 0 0 0 4 2 1 1
Nucleus 0 1 1 1 1 2 5 4 9 11 4 3 3 2 4 1 3 2 0 4 0 6 5 6
Nuclear Periphery 1 0 0 0 0 0 0 3 0 1 2 2 0 0 0 0 0 0 0 0 0 0 0 2
Nucleolus 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1
Vac/Vac Membrane 0 1 3 4 7 14 25 35 54 53 63 60 2 20 2 5 6 2 0 1 3 4 7 5
Unique Cell Count 385 267 391 205 280 281 391 365 460 566 434 446 448 517 479 83 128 117 375 264 390 326 421 449
Labelled Cell Count 390 270 402 212 288 306 441 396 505 638 515 508 454 535 488 88 140 120 375 264 390 326 421 449


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 12.5 13.8 11.8 10.6 10.2 8.6 7.9 8.0 7.1 7.2 6.5 6.6 13.4 12.8 12.4 14.8 12.8 12.7 13.0 13.9 15.2
Std Deviation (1e-4) 1.8 2.1 1.8 2.2 1.7 1.6 1.7 1.8 1.4 1.5 1.5 1.5 2.1 1.9 1.9 2.6 2.9 2.4 2.2 2.4 2.4
Intensity Change (Log2) – – – -0.16 -0.21 -0.46 -0.59 -0.57 -0.73 -0.71 -0.85 -0.84 0.18 0.11 0.07 0.32 0.11 0.1 0.14 0.24 0.37


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 -0.2 -0.2 0.2 -1.4 -0.8 -0.8 -0.2 0.2 0 -1.4 0 0 0 0
Cytoplasm -0.6 -1.7 -4.8 -8.5 -8.6 -10.0 -10.3 -11.4 -11.6 2.2 -0.3 2.7 -1.1 -1.2 0
Endoplasmic Reticulum 0 0 0 1.8 0.9 2.2 1.6 0 1.2 0 -0.5 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 7.3 9.6 8.2 8.6 10.4 12.2 11.4 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 3.4 4.2 5.5 6.4 5.6 7.3 6.9 0 2.9 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 11.0163 13.0999 12.1465 11.3701 10.9265 11.8077 11.7894 13.7753 13.1945 11.7594 12.1779 12.2688 9.1378 11.6034 9.7404 9.4552 9.4725 10.0979
Actin 0.002 0.0004 0.0023 0.0011 0.0002 0.0004 0.0001 0.0007 0.0001 0.0001 0 0.0003 0.0159 0.0002 0.0024 0.0516 0.0397 0.0033
Bud 0.0001 0.0001 0.0003 0.0001 0.0001 0.0001 0.0001 0.0032 0 0.0001 0.0001 0.0002 0.0002 0.0001 0.0002 0.0002 0.0009 0.0001
Bud Neck 0.0003 0.0002 0.0004 0.0004 0.0005 0.001 0.0003 0.0003 0.0003 0.0006 0.0006 0.0009 0.0008 0.0001 0.0005 0.0014 0.0004 0.0007
Bud Periphery 0 0 0.0004 0 0 0 0 0.0015 0 0 0 0.0001 0.0002 0 0.0001 0.0001 0.0013 0
Bud Site 0.0006 0.0003 0.0003 0.0001 0.0001 0 0.0001 0.001 0 0.0001 0 0.0002 0.0014 0.0001 0.0003 0.0004 0.0026 0.0001
Cell Periphery 0.0001 0 0.0001 0 0 0 0 0.0001 0 0 0 0 0.0001 0 0 0.0001 0.0001 0
Cytoplasm 0.9582 0.9738 0.9594 0.9454 0.9644 0.9625 0.9407 0.953 0.9341 0.9438 0.92 0.9309 0.9019 0.9905 0.9471 0.8933 0.904 0.9545
Cytoplasmic Foci 0.0027 0.0012 0.0023 0.0028 0.0013 0.0028 0.0015 0.0021 0.001 0.003 0.0011 0.0015 0.0121 0.0008 0.0025 0.0088 0.0026 0.003
Eisosomes 0 0 0 0 0 0 0 0 0 0 0 0 0.0002 0 0 0.0003 0.0006 0
Endoplasmic Reticulum 0.0063 0.0009 0.0015 0.0019 0.0013 0.0036 0.0031 0.0016 0.0016 0.0021 0.0017 0.0021 0.0053 0.0006 0.0029 0.0078 0.0013 0.0016
Endosome 0.0018 0.0006 0.0016 0.0011 0.0006 0.0022 0.0012 0.0014 0.0008 0.0005 0.0007 0.0011 0.0097 0.0004 0.0064 0.0098 0.0034 0.0027
Golgi 0.0002 0.0001 0.0007 0.0002 0 0.0001 0 0.0001 0 0 0 0 0.0013 0.0001 0.0012 0.0016 0.0027 0.0013
Lipid Particles 0.0001 0 0 0 0 0 0 0 0 0 0 0 0.0019 0 0.0032 0.0019 0.0032 0.002
Mitochondria 0.0002 0.0001 0.0029 0.0002 0.0001 0.0005 0.0001 0.0003 0.0001 0.0001 0.0001 0.0003 0.003 0.0001 0.0014 0.0003 0.0163 0.0002
None 0.0042 0.0009 0.0017 0.0112 0.0012 0.0015 0.0022 0.0015 0.0012 0.0027 0.0012 0.0013 0.0069 0.0008 0.0078 0.0042 0.0022 0.0019
Nuclear Periphery 0.0038 0.0012 0.0019 0.0024 0.0025 0.0025 0.0043 0.0023 0.0031 0.0021 0.0027 0.003 0.009 0.0009 0.0053 0.0038 0.0022 0.0026
Nucleolus 0 0 0 0 0 0 0.0001 0 0 0.0003 0.0001 0 0.0016 0 0.0002 0.0001 0.0001 0
Nucleus 0.0178 0.0195 0.023 0.0316 0.0265 0.0213 0.0443 0.0291 0.0564 0.0265 0.0704 0.0564 0.022 0.0049 0.0138 0.0107 0.0106 0.0224
Peroxisomes 0.0001 0 0.0002 0.0002 0 0.0001 0 0.0001 0 0 0 0 0.0011 0 0.0015 0.0005 0.0036 0.0014
Punctate Nuclear 0.0008 0.0003 0.0004 0.0005 0.0005 0.0004 0.001 0.0007 0.0007 0.0169 0.0006 0.0008 0.0035 0.0002 0.0011 0.0018 0.0006 0.002
Vacuole 0.0006 0.0002 0.0005 0.0004 0.0005 0.0008 0.0007 0.0007 0.0004 0.001 0.0007 0.0007 0.0013 0.0002 0.0017 0.001 0.0011 0.0003
Vacuole Periphery 0.0001 0 0.0002 0.0001 0.0001 0.0002 0.0001 0.0001 0.0001 0.0001 0.0001 0.0002 0.0006 0 0.0005 0.0002 0.0005 0.0001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 28.0114 23.9517 19.389 29.7612 29.4755 25.1557 20.9152 26.9944 21.4595 30.7773
Translational Efficiency 1.4515 1.5749 1.738 1.0465 1.1317 1.4489 1.8869 0.9596 1.4013 1.0323

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1939 286 3065 1612 1847 1788 170 189 3786 2074 3235 1801

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 1104.60 1479.90 1321.46 1370.12 1147.34 1337.28 1369.38 1422.81 1125.45 1356.95 1323.98 1375.65
Standard Deviation 157.02 245.13 181.72 208.60 163.73 202.85 186.18 239.95 161.74 214.89 182.27 212.72
Intensity Change Log 2 — 0.421976 0.258609 0.310778 — 0.221009 0.255230 0.310450 — 0.323083 0.256888 0.310611

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000231 0.000525 0.000065 0.002764 0.000212 0.000323 0.000612 0.000867 0.000222 0.000351 0.000094 0.002565
Bud Neck 0.000673 0.001264 0.001484 0.004653 0.000580 0.000832 0.002498 0.006220 0.000628 0.000892 0.001538 0.004818
Bud Site 0.004922 0.011519 0.001971 0.043406 0.004798 0.006837 0.007542 0.096833 0.004861 0.007483 0.002264 0.049013
Cell Periphery 0.000057 0.000141 0.000027 0.000185 0.000072 0.000131 0.000231 0.000156 0.000064 0.000132 0.000037 0.000182
Cytoplasm 0.839852* 0.827296* 0.745382* 0.629761* 0.849499* 0.848511* 0.516451* 0.551488* 0.844558* 0.845585* 0.733351* 0.621547*
Cytoplasmic Foci 0.074939 0.051562 0.003500 0.024589 0.059993 0.033951 0.018534 0.051355 0.067648 0.036379 0.004290 0.027398
Eisosomes 0.000007 0.000007 0.000002 0.000017 0.000007 0.000006 0.000035 0.000014 0.000007 0.000006 0.000004 0.000016
Endoplasmic Reticulum 0.002120 0.000815 0.000169 0.002404 0.001964 0.000899 0.002373 0.000504 0.002044 0.000888 0.000285 0.002205
Endosome 0.002040 0.002206 0.000186 0.014237 0.001880 0.002791 0.005486 0.013162 0.001962 0.002710 0.000465 0.014125
Golgi 0.002175 0.003488 0.000065 0.015564 0.001471 0.002230 0.000265 0.004702 0.001832 0.002403 0.000076 0.014424
Lipid Particles 0.000838 0.001222 0.000174 0.002307 0.000791 0.000602 0.002341 0.002579 0.000815 0.000687 0.000288 0.002335
Mitochondria 0.000837 0.000075 0.000316 0.005645 0.000439 0.001029 0.000193 0.017083 0.000643 0.000897 0.000310 0.006846
Mitotic Spindle 0.003186 0.004956 0.003652 0.074759 0.001963 0.012937 0.009572 0.084871 0.002589 0.011837 0.003963 0.075821
None 0.002391 0.003239 0.007647 0.003354 0.001997 0.003296 0.003534 0.003543 0.002199 0.003288 0.007431 0.003374
Nuclear Periphery 0.000128 0.000157 0.000319 0.000552 0.000106 0.000163 0.001188 0.000397 0.000117 0.000162 0.000365 0.000536
Nuclear Periphery Foci 0.001902 0.009184 0.000415 0.006016 0.002478 0.003982 0.012975 0.003815 0.002183 0.004699 0.001075 0.005785
Nucleolus 0.000265 0.000098 0.000107 0.000197 0.000101 0.000207 0.000172 0.000143 0.000185 0.000192 0.000110 0.000191
Nucleus 0.040276 0.051511 0.223686* 0.091038 0.048760 0.046987 0.175268* 0.047835 0.044415 0.047611 0.221142* 0.086504
Peroxisomes 0.000355 0.000495 0.000058 0.002802 0.000183 0.000545 0.000202 0.004524 0.000271 0.000538 0.000065 0.002982
Vacuole 0.021349 0.028337 0.010677 0.064961 0.021782 0.032271 0.240315* 0.106611 0.021560 0.031729 0.022744 0.069331
Vacuole Periphery 0.001458 0.001903 0.000099 0.010788 0.000925 0.001470 0.000216 0.003297 0.001198 0.001529 0.000105 0.010002

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -3.37 2.25 -5.54 -3.80 -6.15 -1.95 -8.74 -3.06 -2.16 1.20 -2.56 1.83 -5.87 -5.05 -6.22
Bud Neck -4.03 -3.78 -12.52 -8.60 -6.24 -3.43 -8.09 -4.83 -4.51 -1.75 -4.16 -4.59 -13.56 -11.90 -6.62
Bud Site -2.51 4.35 -8.50 -3.39 -10.26 -1.78 -4.37 -3.40 -3.14 -2.75 -2.58 4.73 -9.31 -7.43 -10.64
Cell Periphery -6.25 6.27 -10.68 -0.66 -13.69 -0.80 -9.34 -6.39 -0.62 4.55 -1.17 5.75 -11.14 -0.78 -13.41
Cytoplasm 2.35 25.95 33.33 18.58 16.24 3.07 22.72 14.89 13.94 -4.15 3.04 34.88 38.87 32.92 15.26
Cytoplasmic Foci 3.88 26.69 18.66 6.55 -14.02 9.12 13.73 3.55 -1.09 -4.22 13.05 35.60 21.14 7.56 -15.02
Eisosomes -0.95 7.65 -9.18 -6.21 -13.51 -0.04 -12.87 -7.04 -6.85 7.96 -0.43 3.72 -10.46 -9.29 -12.30
Endoplasmic Reticulum 3.00 4.83 2.62 -0.20 -2.22 3.53 -1.87 5.97 2.27 9.15 4.20 7.14 3.59 0.07 -1.77
Endosome -0.16 11.04 -2.79 -2.43 -5.78 -2.46 -5.25 -2.00 -1.21 0.07 -2.29 13.36 -3.31 -1.87 -6.00
Golgi -0.28 9.41 -1.24 -0.22 -3.92 -1.20 7.16 1.48 2.04 -1.10 -0.53 12.53 -1.53 -1.06 -4.00
Lipid Particles -2.15 6.67 -9.32 -6.79 -10.88 0.94 -7.09 -6.64 -6.84 -1.00 0.43 5.12 -10.36 -10.32 -11.41
Mitochondria 2.45 1.57 -0.77 -2.33 -1.81 -1.52 1.02 -0.82 -0.56 -0.90 -0.48 1.71 -1.35 -1.05 -2.01
Mitotic Spindle -0.45 -2.31 -9.28 -8.04 -8.58 -5.16 -3.51 -3.79 -2.55 -2.11 -4.90 -4.08 -10.26 -6.79 -8.99
None -1.17 -16.03 -3.49 -0.77 15.95 -2.80 -1.25 -3.51 -2.24 -2.96 -2.53 -21.35 -6.14 -1.65 14.12
Nuclear Periphery -1.62 -14.92 -16.32 -14.38 -6.11 -3.41 -11.55 -9.32 -7.56 7.90 -3.12 -21.15 -19.24 -16.40 -4.08
Nuclear Periphery Foci -5.47 8.27 -5.13 3.52 -8.26 -4.01 -7.32 -3.69 -0.15 5.29 -6.69 5.88 -5.12 1.19 -7.68
Nucleolus 1.45 1.10 -0.10 -5.41 -5.06 -2.81 -5.07 -2.75 0.64 0.05 -0.30 0.56 -1.45 -1.52 -5.04
Nucleus -3.28 -60.58 -23.72 -12.47 29.33 -0.86 -8.80 -2.47 -2.17 7.16 -3.90 -60.83 -22.77 -19.58 31.15
Peroxisomes -0.27 3.00 -3.72 -2.54 -5.56 -3.19 -1.07 -2.46 -2.04 -2.22 -2.22 3.72 -4.95 -3.53 -6.06
Vacuole -3.57 -3.50 -30.70 -20.69 -29.38 -9.18 -15.35 -13.13 -11.83 2.55 -10.00 -10.33 -33.44 -27.22 -27.33
Vacuole Periphery -0.01 4.50 -1.11 -0.57 -3.74 -0.82 3.45 1.13 1.59 -0.97 -0.27 5.87 -1.48 -1.10 -3.80
Standard name
Human Ortholog
Description Prephenate dehydrogenase involved in tyrosine biosynthesis; expression is dependent on phenylalanine levels
Localization
Cell Percentages cytoplasm (100%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

Tyr1

Tyr1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Tyr1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available