Standard name | |
---|---|
Human Ortholog | |
Description | Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S6, no bacterial homolog; phosphorylated on S233 by Ypk3p in a TORC1-dependent manner, and on S232 in a TORC1/2-dependent manner by Ypk1/2/3p; RPS6B has a paralog, RPS6A, that arose from the whole genome duplication |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 1 | 2 | 1 | 9 | 9 | 9 | 6 | 5 | 0 | – | 1 | 0 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 1 | 4 | 4 | 4 | 6 | 12 | 10 | 6 | 9 | 5 | 0 | – | 4 | 2 | 18 | 2 | 8 | 0 | 0 | 0 | 0 | 0 | 1 |
Cytoplasm | 146 | 133 | 108 | 124 | 129 | 102 | 163 | 128 | 88 | 105 | 58 | 40 | – | 118 | 114 | 250 | 199 | 264 | 142 | 151 | 116 | 96 | 94 | 149 |
Endoplasmic Reticulum | 3 | 1 | 6 | 7 | 10 | 6 | 23 | 40 | 15 | 20 | 9 | 5 | – | 1 | 4 | 8 | 8 | 10 | 3 | 2 | 5 | 11 | 14 | 11 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 |
Golgi | 0 | 0 | 0 | 1 | 3 | 2 | 4 | 2 | 3 | 3 | 0 | 2 | – | 0 | 0 | 2 | 4 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 1 | 0 | 6 | 0 | 17 | 37 | 30 | 60 | 52 | 53 | 27 | – | 0 | 0 | 0 | 4 | 8 | 2 | 0 | 1 | 0 | 5 | 3 |
Nucleus | 0 | 0 | 0 | 1 | 1 | 2 | 2 | 2 | 0 | 1 | 0 | 2 | – | 0 | 2 | 1 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 1 | 0 | 4 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 1 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vac/Vac Membrane | 0 | 1 | 0 | 5 | 7 | 4 | 10 | 6 | 5 | 12 | 5 | 5 | – | 1 | 0 | 12 | 13 | 4 | 0 | 3 | 2 | 1 | 2 | 2 |
Unique Cell Count | 149 | 136 | 114 | 137 | 149 | 123 | 223 | 199 | 154 | 179 | 119 | 75 | 122 | 116 | 270 | 213 | 274 | 153 | 158 | 128 | 114 | 121 | 173 | |
Labelled Cell Count | 149 | 137 | 118 | 149 | 156 | 140 | 263 | 228 | 187 | 209 | 140 | 82 | 125 | 122 | 292 | 232 | 295 | 153 | 158 | 128 | 114 | 121 | 173 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 15.8 | 22.8 | 15.4 | 17.0 | 16.6 | 13.4 | 12.9 | 12.7 | 11.8 | 11.9 | 11.1 | 10.8 | – | 18.9 | 18.2 | 24.3 | 24.5 | 25.4 | 18.0 | 15.6 | 18.0 |
Std Deviation (1e-4) | 2.9 | 4.0 | 2.5 | 2.9 | 3.0 | 3.1 | 3.1 | 2.8 | 2.5 | 2.8 | 2.7 | 2.4 | – | 3.2 | 3.6 | 6.1 | 6.1 | 6.4 | 4.2 | 4.0 | 4.4 |
Intensity Change (Log2) | – | – | – | 0.14 | 0.11 | -0.2 | -0.25 | -0.28 | -0.38 | -0.37 | -0.47 | -0.5 | – | 0.3 | 0.25 | 0.66 | 0.67 | 0.73 | 0.23 | 0.02 | 0.23 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0.8 | 0.6 | 0 | 0.6 | 0 | 0 | – | 0 | 0 | 1.2 | 0 | 0 |
Cytoplasm | -1.3 | -2.2 | -2.9 | -4.7 | -6.0 | -6.9 | -6.8 | -7.8 | -6.8 | – | 0.8 | 1.5 | -0.8 | -0.5 | 0.7 |
Endoplasmic Reticulum | -0.1 | 0.5 | -0.1 | 1.6 | 3.6 | 1.3 | 1.7 | 0.7 | 0 | – | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 4.1 | 4.6 | 4.4 | 7.6 | 6.3 | 8.1 | 6.9 | – | 0 | 0 | 0 | 0 | 0 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 26.5211 | 34.9736 | 32.25 | 31.2431 | 25.0575 | 32.0047 | 27.232 | 33.8451 | 31.8206 | 27.9101 | 29.4427 | 30.8481 | 28.2795 | 37.4315 | 32.7429 | 23.9491 | 28.6654 | 32.9587 |
Actin | 0.0046 | 0.0015 | 0.0004 | 0.001 | 0.0044 | 0.0008 | 0.004 | 0.0011 | 0.0005 | 0.0003 | 0.0005 | 0.0016 | 0.0013 | 0.0038 | 0.0019 | 0.005 | 0.0022 | 0.0022 |
Bud | 0.0012 | 0.0005 | 0.0006 | 0.0051 | 0.0031 | 0.0008 | 0.0008 | 0.0004 | 0.0006 | 0.0009 | 0.0009 | 0.0012 | 0.005 | 0.006 | 0.0068 | 0.0203 | 0.009 | 0.0044 |
Bud Neck | 0.0011 | 0.0004 | 0.0006 | 0.0008 | 0.0009 | 0.0013 | 0.0006 | 0.0004 | 0.0007 | 0.0012 | 0.0011 | 0.0015 | 0.0014 | 0.0012 | 0.0016 | 0.0026 | 0.0028 | 0.0048 |
Bud Periphery | 0.0022 | 0.0008 | 0.0004 | 0.0227 | 0.0061 | 0.0008 | 0.0011 | 0.0002 | 0.0007 | 0.0004 | 0.0008 | 0.0018 | 0.0087 | 0.0042 | 0.0092 | 0.0224 | 0.0155 | 0.0097 |
Bud Site | 0.001 | 0.0006 | 0.0004 | 0.0007 | 0.002 | 0.0001 | 0.0005 | 0.0004 | 0.0003 | 0.0002 | 0.0001 | 0.0001 | 0.0024 | 0.0074 | 0.005 | 0.0036 | 0.0016 | 0.0005 |
Cell Periphery | 0.0041 | 0.0031 | 0.0014 | 0.0018 | 0.0053 | 0.0009 | 0.0047 | 0.0006 | 0.0012 | 0.0009 | 0.0005 | 0.0022 | 0.0073 | 0.0035 | 0.0103 | 0.0048 | 0.0031 | 0.0054 |
Cytoplasm | 0.7342 | 0.9292 | 0.8731 | 0.8014 | 0.7014 | 0.8285 | 0.7535 | 0.9226 | 0.8729 | 0.8707 | 0.8226 | 0.7932 | 0.6743 | 0.8527 | 0.727 | 0.5542 | 0.7133 | 0.6935 |
Cytoplasmic Foci | 0.0073 | 0.0034 | 0.0095 | 0.0054 | 0.0257 | 0.0095 | 0.0057 | 0.0052 | 0.0066 | 0.0097 | 0.0136 | 0.0108 | 0.004 | 0.0031 | 0.0042 | 0.0046 | 0.0089 | 0.0068 |
Eisosomes | 0.0001 | 0.0001 | 0 | 0 | 0.0005 | 0 | 0.0001 | 0 | 0 | 0 | 0 | 0 | 0 | 0.0001 | 0 | 0 | 0 | 0.0001 |
Endoplasmic Reticulum | 0.0823 | 0.0244 | 0.0223 | 0.0084 | 0.0277 | 0.0707 | 0.1063 | 0.0319 | 0.0378 | 0.0175 | 0.0474 | 0.0875 | 0.1042 | 0.0397 | 0.045 | 0.0394 | 0.036 | 0.1188 |
Endosome | 0.0367 | 0.0075 | 0.0244 | 0.0232 | 0.0536 | 0.0411 | 0.0322 | 0.0132 | 0.0221 | 0.0392 | 0.067 | 0.0439 | 0.0132 | 0.0072 | 0.0086 | 0.017 | 0.0346 | 0.0294 |
Golgi | 0.0057 | 0.0022 | 0.0023 | 0.0028 | 0.0049 | 0.0069 | 0.0055 | 0.0046 | 0.0024 | 0.0038 | 0.0092 | 0.0132 | 0.0016 | 0.0021 | 0.0018 | 0.001 | 0.0035 | 0.0073 |
Lipid Particles | 0.0013 | 0.0004 | 0.0014 | 0.0004 | 0.0118 | 0.0002 | 0.0006 | 0.0002 | 0.0003 | 0.0002 | 0.0001 | 0.0002 | 0.001 | 0.0004 | 0.0015 | 0.0009 | 0.0012 | 0.0004 |
Mitochondria | 0.011 | 0.0045 | 0.0021 | 0.0126 | 0.0172 | 0.007 | 0.0051 | 0.0017 | 0.0016 | 0.0005 | 0.0059 | 0.0175 | 0.0032 | 0.001 | 0.0025 | 0.0019 | 0.0018 | 0.0113 |
None | 0.0038 | 0.0015 | 0.003 | 0.0009 | 0.0158 | 0.0006 | 0.0008 | 0.0002 | 0.0003 | 0.0002 | 0.0003 | 0.0006 | 0.0012 | 0.0006 | 0.0016 | 0.0013 | 0.0014 | 0.0007 |
Nuclear Periphery | 0.0162 | 0.0017 | 0.0042 | 0.0036 | 0.0166 | 0.0035 | 0.0113 | 0.0014 | 0.003 | 0.003 | 0.0021 | 0.0038 | 0.016 | 0.0054 | 0.0111 | 0.0261 | 0.0179 | 0.0095 |
Nucleolus | 0.0013 | 0.0001 | 0.0018 | 0.0003 | 0.0022 | 0 | 0.0001 | 0.0001 | 0 | 0 | 0 | 0 | 0.001 | 0.0007 | 0.0011 | 0.0037 | 0.0021 | 0.001 |
Nucleus | 0.007 | 0.0012 | 0.0044 | 0.0025 | 0.0082 | 0.0015 | 0.003 | 0.0018 | 0.0013 | 0.0018 | 0.0012 | 0.0012 | 0.0765 | 0.0322 | 0.0855 | 0.1284 | 0.0647 | 0.046 |
Peroxisomes | 0.0007 | 0.0001 | 0.0002 | 0.0003 | 0.0007 | 0.0001 | 0.0006 | 0 | 0 | 0.0001 | 0.0001 | 0.0001 | 0.0007 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0001 |
Punctate Nuclear | 0.0022 | 0.0001 | 0.001 | 0.0001 | 0.0067 | 0.0004 | 0.0001 | 0 | 0.0001 | 0 | 0 | 0.0001 | 0.0058 | 0.0018 | 0.0101 | 0.0078 | 0.0083 | 0.0062 |
Vacuole | 0.063 | 0.0147 | 0.0377 | 0.0911 | 0.0627 | 0.0186 | 0.054 | 0.0129 | 0.042 | 0.0436 | 0.0202 | 0.015 | 0.0615 | 0.0247 | 0.0591 | 0.141 | 0.0627 | 0.0335 |
Vacuole Periphery | 0.0132 | 0.0021 | 0.0087 | 0.015 | 0.0225 | 0.0067 | 0.0095 | 0.0012 | 0.0057 | 0.0057 | 0.0065 | 0.0044 | 0.0094 | 0.0021 | 0.0059 | 0.0136 | 0.0093 | 0.0083 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 3217.099 | 3609.7761 | 4404.9848 | 3396.6013 | 4039.3161 | 3894.6761 | 3130.8397 | 2957.1173 | 3409.6389 | 2838.0761 |
Translational Efficiency | 0.9071 | 0.9111 | 0.789 | 1.0834 | 0.9361 | 0.7032 | 0.8985 | 1.0558 | 0.8812 | 0.9719 |
Dataset | Hit |
---|---|
Protein Concentration | ✔ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
1876 | 137 | 2262 | 946 | 2007 | 2055 | 2240 | 206 | 3883 | 2192 | 4502 | 1152 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 1300.77 | 1943.60 | 1882.67 | 2108.27 | 1509.86 | 1679.91 | 2078.32 | 1963.66 | 1408.84 | 1696.39 | 1980.02 | 2082.41 |
Standard Deviation | 229.71 | 328.39 | 322.88 | 350.23 | 245.16 | 326.68 | 321.11 | 376.72 | 259.76 | 332.96 | 336.53 | 359.41 |
Intensity Change Log 2 | — | 0.579365 | 0.533414 | 0.696694 | — | 0.153969 | 0.461003 | 0.379130 | — | 0.366494 | 0.494968 | 0.534819 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.000101 | 0.000336 | 0.000107 | 0.000402 | 0.000111 | 0.000147 | 0.000099 | 0.000367 | 0.000106 | 0.000159 | 0.000103 | 0.000396 |
Bud Neck | 0.000121 | 0.001582 | 0.000525 | 0.001045 | 0.000164 | 0.000166 | 0.000585 | 0.002100 | 0.000143 | 0.000254 | 0.000555 | 0.001233 |
Bud Site | 0.012372 | 0.025806 | 0.007459 | 0.017139 | 0.010127 | 0.010166 | 0.006945 | 0.028378 | 0.011212 | 0.011143 | 0.007203 | 0.019149 |
Cell Periphery | 0.000596 | 0.007687 | 0.001462 | 0.015554 | 0.000569 | 0.000649 | 0.002430 | 0.004629 | 0.000582 | 0.001089 | 0.001944 | 0.013600 |
Cytoplasm | 0.793962* | 0.695108* | 0.856561* | 0.664887* | 0.751162* | 0.853952* | 0.796870* | 0.762942* | 0.771840* | 0.844024* | 0.826861* | 0.682421* |
Cytoplasmic Foci | 0.098091 | 0.062342 | 0.018315 | 0.019352 | 0.104387 | 0.053114 | 0.017616 | 0.037744 | 0.101345 | 0.053691 | 0.017967 | 0.022641 |
Eisosomes | 0.000002 | 0.000004 | 0.000001 | 0.000004 | 0.000001 | 0.000002 | 0.000001 | 0.000004 | 0.000002 | 0.000002 | 0.000001 | 0.000004 |
Endoplasmic Reticulum | 0.004288 | 0.003813 | 0.001686 | 0.002710 | 0.006994 | 0.002449 | 0.002351 | 0.000875 | 0.005687 | 0.002534 | 0.002017 | 0.002382 |
Endosome | 0.003460 | 0.008794 | 0.002908 | 0.003836 | 0.005074 | 0.003960 | 0.002773 | 0.007158 | 0.004294 | 0.004263 | 0.002841 | 0.004430 |
Golgi | 0.024521 | 0.092304 | 0.031342 | 0.090060 | 0.035775 | 0.032421 | 0.043559 | 0.039674 | 0.030338 | 0.036164 | 0.037421 | 0.081050 |
Lipid Particles | 0.000589 | 0.017281 | 0.003273 | 0.022426 | 0.000692 | 0.001429 | 0.004571 | 0.010404 | 0.000643 | 0.002420 | 0.003919 | 0.020276 |
Mitochondria | 0.000795 | 0.000108 | 0.000158 | 0.000194 | 0.000507 | 0.000609 | 0.000171 | 0.000048 | 0.000646 | 0.000578 | 0.000164 | 0.000168 |
Mitotic Spindle | 0.000723 | 0.002948 | 0.002906 | 0.001828 | 0.001542 | 0.002240 | 0.001790 | 0.002313 | 0.001146 | 0.002284 | 0.002351 | 0.001915 |
None | 0.001461 | 0.000231 | 0.000401 | 0.000440 | 0.000415 | 0.000367 | 0.000393 | 0.000215 | 0.000921 | 0.000358 | 0.000397 | 0.000400 |
Nuclear Periphery | 0.000024 | 0.000095 | 0.000088 | 0.000192 | 0.000048 | 0.000026 | 0.000125 | 0.000046 | 0.000037 | 0.000030 | 0.000107 | 0.000166 |
Nuclear Periphery Foci | 0.001092 | 0.010443 | 0.005662 | 0.009638 | 0.001763 | 0.001408 | 0.006979 | 0.004058 | 0.001439 | 0.001973 | 0.006317 | 0.008640 |
Nucleolus | 0.000097 | 0.000032 | 0.000033 | 0.000067 | 0.000017 | 0.000112 | 0.000045 | 0.000083 | 0.000055 | 0.000107 | 0.000039 | 0.000070 |
Nucleus | 0.002530 | 0.001185 | 0.002081 | 0.002960 | 0.000696 | 0.001093 | 0.001893 | 0.003261 | 0.001582 | 0.001098 | 0.001987 | 0.003014 |
Peroxisomes | 0.000093 | 0.000230 | 0.000042 | 0.000096 | 0.000055 | 0.000205 | 0.000044 | 0.000415 | 0.000074 | 0.000207 | 0.000043 | 0.000153 |
Vacuole | 0.012593 | 0.030533 | 0.038058 | 0.089780 | 0.017704 | 0.010717 | 0.060920 | 0.081042 | 0.015235 | 0.011956 | 0.049433 | 0.088218 |
Vacuole Periphery | 0.042488 | 0.039137 | 0.026932 | 0.057388 | 0.062195 | 0.024766 | 0.049841 | 0.014242 | 0.052674 | 0.025664 | 0.038331 | 0.049673 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | -3.67 | -0.65 | -6.43 | -0.97 | -6.21 | -1.54 | 0.20 | -3.89 | -3.36 | -3.97 | -2.69 | -0.21 | -7.22 | -5.62 | -7.19 |
Bud Neck | -3.15 | -7.86 | -12.22 | 1.07 | -5.49 | -0.11 | -6.18 | -5.65 | -5.65 | -4.44 | -3.28 | -9.57 | -11.90 | -10.33 | -6.68 |
Bud Site | -3.01 | 5.79 | -3.30 | 1.84 | -6.70 | -0.06 | 3.96 | -3.75 | -3.74 | -4.37 | 0.07 | 6.88 | -5.47 | -5.22 | -8.17 |
Cell Periphery | -6.00 | -6.59 | -12.26 | -4.56 | -11.49 | -1.13 | -11.26 | -4.22 | -4.13 | -2.11 | -4.81 | -12.90 | -12.70 | -12.14 | -11.30 |
Cytoplasm | 3.79 | -8.24 | 10.65 | 1.09 | 16.40 | -13.18 | -5.31 | -0.39 | 5.05 | 1.90 | -11.05 | -9.56 | 8.79 | 15.27 | 14.24 |
Cytoplasmic Foci | 4.04 | 22.07 | 21.36 | 5.25 | -0.65 | 13.59 | 26.83 | 12.21 | 3.14 | -4.40 | 15.37 | 34.49 | 29.71 | 13.18 | -3.40 |
Eisosomes | -2.37 | 3.65 | -5.04 | -0.82 | -7.12 | -2.46 | 1.47 | -3.30 | -1.99 | -3.57 | -2.12 | 3.86 | -6.51 | -3.79 | -7.90 |
Endoplasmic Reticulum | 0.47 | 5.90 | 3.25 | 1.16 | -2.80 | 5.13 | 5.73 | 7.73 | 3.44 | 5.04 | 5.23 | 7.80 | 6.57 | 0.35 | -1.16 |
Endosome | -4.18 | 1.55 | -1.11 | 3.89 | -2.69 | 2.33 | 7.77 | -1.63 | -2.45 | -3.53 | 0.06 | 6.54 | -0.34 | -0.28 | -4.68 |
Golgi | -6.41 | -4.04 | -14.62 | 0.29 | -12.72 | 1.65 | -3.58 | -0.17 | -0.72 | 1.10 | -3.17 | -5.13 | -12.94 | -10.66 | -10.84 |
Lipid Particles | -3.93 | -8.81 | -10.06 | -1.06 | -8.71 | -2.22 | -11.71 | -7.13 | -6.40 | -4.12 | -4.26 | -14.55 | -10.89 | -9.64 | -8.96 |
Mitochondria | 4.56 | 4.59 | 4.12 | -1.10 | -0.70 | -0.53 | 2.54 | 3.48 | 3.82 | 4.89 | 0.40 | 5.03 | 4.65 | 2.88 | -0.05 |
Mitotic Spindle | -4.16 | -5.13 | -5.13 | 1.95 | 2.40 | -1.67 | -1.21 | -1.50 | -0.83 | -1.16 | -2.95 | -4.83 | -3.36 | 0.47 | 0.99 |
None | 2.80 | 2.41 | 2.33 | -5.73 | -1.22 | 0.49 | 0.24 | 1.95 | 2.26 | 3.26 | 2.55 | 2.41 | 2.40 | -0.89 | -0.03 |
Nuclear Periphery | -4.06 | -10.34 | -10.30 | -4.12 | -5.88 | 3.51 | -7.78 | -1.00 | -3.94 | 6.40 | 1.36 | -11.90 | -9.58 | -9.87 | -4.00 |
Nuclear Periphery Foci | -5.09 | -11.73 | -14.67 | 0.43 | -5.65 | 1.82 | -11.70 | -3.95 | -4.68 | 4.55 | -2.84 | -16.46 | -14.59 | -13.10 | -3.90 |
Nucleolus | 1.55 | 1.52 | 0.67 | -5.20 | -7.15 | -1.07 | -13.34 | -5.36 | 0.30 | -3.15 | -0.62 | 0.78 | -0.81 | 0.45 | -6.96 |
Nucleus | 2.00 | 0.54 | -0.78 | -5.29 | -2.66 | -1.40 | -9.31 | -6.21 | -5.00 | -3.95 | 1.13 | -1.57 | -4.11 | -5.59 | -4.10 |
Peroxisomes | -1.64 | 4.45 | -0.16 | 1.61 | -4.45 | -1.68 | 1.52 | -5.43 | -1.89 | -5.60 | -1.59 | 4.57 | -4.79 | 0.64 | -6.94 |
Vacuole | -3.50 | -13.32 | -16.67 | -8.77 | -10.58 | 5.32 | -16.51 | -7.21 | -7.96 | -2.51 | 3.04 | -21.00 | -17.80 | -18.46 | -9.04 |
Vacuole Periphery | 0.39 | 6.09 | -3.70 | -1.96 | -8.73 | 12.49 | 3.72 | 14.80 | 4.64 | 12.50* | 11.92 | 6.77 | 1.15 | -7.66 | -3.65 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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