Standard name | |
---|---|
Human Ortholog | |
Description | Calcium and manganese transporter with higher affinity for Ca2+; involved in Ca2+ and Mn2+ homeostasis; localizes to the cis- and medial-Golgi apparatus; GFP-fusion localizes to the vacuole; required for the Mn2+-dependent function of glycosylation enzymes; TMEM165, a human ortholog linked to Congenital Disorders of Glycosylation, functionally complements the null allele; expression pattern and physical interactions suggest a possible role in ribosome biogenesis; expression regulated by Gcr1p |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 1 |
Bud | 0 | 0 | 0 | 0 | 1 | 1 | 1 | 2 | 1 | 1 | 0 | 3 | 0 | 3 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 3 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 58 | 49 | 57 | 70 | 101 | 98 | 172 | 177 | 210 | 200 | 93 | 145 | 44 | 127 | 145 | 36 | 34 | 27 | 5 | 4 | 4 | 0 | 2 | 3 |
Endoplasmic Reticulum | 0 | 3 | 1 | 1 | 3 | 0 | 6 | 1 | 8 | 2 | 4 | 1 | 6 | 14 | 6 | 3 | 7 | 3 | 0 | 3 | 1 | 1 | 1 | 5 |
Endosome | 0 | 2 | 1 | 1 | 1 | 1 | 0 | 1 | 1 | 2 | 0 | 2 | 0 | 1 | 0 | 5 | 9 | 14 | 1 | 5 | 5 | 1 | 5 | 6 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 3 | 2 | 0 | 0 | 0 | 0 | 1 | 2 |
Mitochondria | 3 | 0 | 19 | 3 | 9 | 6 | 45 | 30 | 29 | 42 | 21 | 29 | 0 | 0 | 0 | 2 | 1 | 2 | 2 | 0 | 1 | 1 | 2 | 4 |
Nucleus | 3 | 3 | 4 | 3 | 2 | 4 | 13 | 10 | 4 | 8 | 0 | 4 | 6 | 2 | 2 | 12 | 14 | 14 | 2 | 0 | 2 | 1 | 2 | 1 |
Nuclear Periphery | 0 | 0 | 0 | 1 | 1 | 0 | 2 | 0 | 1 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 2 | 3 | 0 | 0 | 0 | 1 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 |
Vac/Vac Membrane | 28 | 70 | 69 | 49 | 94 | 74 | 111 | 143 | 141 | 129 | 45 | 74 | 18 | 27 | 9 | 67 | 100 | 121 | 76 | 115 | 126 | 72 | 97 | 111 |
Unique Cell Count | 75 | 104 | 125 | 105 | 179 | 151 | 290 | 308 | 326 | 314 | 137 | 210 | 64 | 159 | 157 | 105 | 143 | 153 | 92 | 131 | 145 | 84 | 117 | 145 |
Labelled Cell Count | 92 | 127 | 151 | 128 | 213 | 184 | 350 | 365 | 396 | 385 | 163 | 260 | 74 | 176 | 163 | 127 | 171 | 186 | 92 | 131 | 145 | 84 | 117 | 145 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 5.1 | 7.5 | 6.0 | 6.3 | 6.0 | 6.0 | 5.2 | 5.4 | 5.6 | 5.5 | 5.8 | 5.9 | 7.5 | 6.1 | 5.9 | 8.9 | 9.4 | 9.4 | 7.9 | 7.9 | 8.3 |
Std Deviation (1e-4) | 1.4 | 1.6 | 1.6 | 1.6 | 1.5 | 1.8 | 1.8 | 1.7 | 1.7 | 1.6 | 1.9 | 1.8 | 1.5 | 1.2 | 0.9 | 2.3 | 2.3 | 2.5 | 1.7 | 1.8 | 1.7 |
Intensity Change (Log2) | – | – | – | 0.07 | -0.0 | 0.01 | -0.2 | -0.15 | -0.1 | -0.12 | -0.06 | -0.03 | 0.32 | 0.03 | -0.02 | 0.57 | 0.64 | 0.65 | 0.4 | 0.39 | 0.46 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 3.2 | 1.9 | 3.2 | 2.6 | 2.2 | 3.6 | 3.5 | 3.6 | 4.2 | 3.0 | 6.0 | 8.7 | -1.7 | -3.8 | -5.0 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3.0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3.1 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | -3.2 | -3.0 | -3.2 | 0.1 | -1.6 | -1.9 | -0.5 | 0 | -0.4 | -3.3 | -5.1 | -5.1 | -3.5 | -4.5 | -4.4 |
Nucleus | 0 | 0 | 0 | 0.6 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2.4 | 2.2 | 2.0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | -1.3 | -0.5 | -1.0 | -3.2 | -1.7 | -2.3 | -2.7 | -3.6 | -3.6 | -3.5 | -6.8 | -9.2 | 1.3 | 2.5 | 4.3 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 1.4236 | 3.2638 | 2.881 | 2.6646 | 2.9032 | 2.7406 | 3.2764 | 5.7492 | 5.2072 | 5.1214 | 5.0644 | 5.1834 | 0.9196 | 3.2954 | 2.7733 | 2.938 | 2.7331 | 2.7501 |
Actin | 0.0182 | 0.0001 | 0.0175 | 0 | 0.0026 | 0.0035 | 0.0075 | 0 | 0.001 | 0 | 0.0084 | 0.0001 | 0.0032 | 0 | 0.0156 | 0 | 0.0037 | 0.0001 |
Bud | 0.0005 | 0.0012 | 0.0018 | 0.0003 | 0.003 | 0.0009 | 0.0006 | 0.0006 | 0.0004 | 0.0005 | 0.0003 | 0.0004 | 0.0005 | 0.0003 | 0.0005 | 0.0002 | 0.0006 | 0.0005 |
Bud Neck | 0.0016 | 0.0026 | 0.0011 | 0.0007 | 0.0017 | 0.0023 | 0.0029 | 0.001 | 0.0027 | 0.0007 | 0.0012 | 0.0018 | 0.0012 | 0.0015 | 0.0013 | 0.0007 | 0.0021 | 0.0015 |
Bud Periphery | 0.0007 | 0.0033 | 0.0026 | 0.0008 | 0.0067 | 0.0018 | 0.0014 | 0.0012 | 0.001 | 0.0008 | 0.0006 | 0.0013 | 0.001 | 0.0007 | 0.0009 | 0.0003 | 0.0012 | 0.0018 |
Bud Site | 0.0058 | 0.0043 | 0.0109 | 0.0007 | 0.0021 | 0.0011 | 0.0031 | 0.0047 | 0.0033 | 0.0009 | 0.0013 | 0.0008 | 0.0138 | 0.0018 | 0.0016 | 0.0006 | 0.002 | 0.0006 |
Cell Periphery | 0.0012 | 0.0032 | 0.0028 | 0.0013 | 0.0047 | 0.002 | 0.0023 | 0.0018 | 0.0019 | 0.0007 | 0.0006 | 0.0028 | 0.0032 | 0.0022 | 0.0033 | 0.0022 | 0.0012 | 0.0016 |
Cytoplasm | 0.0578 | 0.0521 | 0.0316 | 0.0072 | 0.013 | 0.024 | 0.0182 | 0.0251 | 0.0474 | 0.0076 | 0.0088 | 0.0116 | 0.0209 | 0.0275 | 0.0291 | 0.0061 | 0.0064 | 0.0118 |
Cytoplasmic Foci | 0.0248 | 0.0112 | 0.0248 | 0.0051 | 0.0114 | 0.0185 | 0.0176 | 0.0114 | 0.0159 | 0.008 | 0.0108 | 0.005 | 0.0118 | 0.006 | 0.02 | 0.0121 | 0.029 | 0.0034 |
Eisosomes | 0.0003 | 0 | 0.0002 | 0 | 0.0001 | 0 | 0.0002 | 0 | 0.0001 | 0 | 0.0002 | 0 | 0.0001 | 0 | 0.0004 | 0 | 0 | 0 |
Endoplasmic Reticulum | 0.0237 | 0.017 | 0.0305 | 0.0077 | 0.0072 | 0.0133 | 0.0176 | 0.0246 | 0.0334 | 0.004 | 0.0064 | 0.0148 | 0.0263 | 0.0416 | 0.0291 | 0.0024 | 0.0095 | 0.0151 |
Endosome | 0.2906 | 0.2151 | 0.3371 | 0.1849 | 0.2568 | 0.1686 | 0.2889 | 0.199 | 0.2991 | 0.2227 | 0.2283 | 0.1644 | 0.3106 | 0.1754 | 0.3236 | 0.319 | 0.191 | 0.1675 |
Golgi | 0.0108 | 0.0104 | 0.0136 | 0.0024 | 0.0285 | 0.0053 | 0.0147 | 0.0048 | 0.0075 | 0.0031 | 0.0105 | 0.0052 | 0.0085 | 0.0031 | 0.019 | 0.0058 | 0.0088 | 0.0046 |
Lipid Particles | 0.017 | 0.0068 | 0.0138 | 0.0019 | 0.0057 | 0.0039 | 0.0148 | 0.0126 | 0.0063 | 0.0015 | 0.0282 | 0.0057 | 0.0159 | 0.0054 | 0.0221 | 0.0205 | 0.0157 | 0.0109 |
Mitochondria | 0.0216 | 0.0028 | 0.0145 | 0.0012 | 0.0161 | 0.005 | 0.0204 | 0.0019 | 0.0139 | 0.0025 | 0.0106 | 0.0047 | 0.0132 | 0.0033 | 0.0122 | 0.0007 | 0.0282 | 0.0032 |
None | 0.0186 | 0.0144 | 0.0252 | 0.0326 | 0.0087 | 0.0205 | 0.0099 | 0.0006 | 0.0044 | 0.0005 | 0.0035 | 0.0035 | 0.0021 | 0.0011 | 0.0099 | 0.0001 | 0.0002 | 0.0002 |
Nuclear Periphery | 0.0286 | 0.013 | 0.0139 | 0.0063 | 0.0154 | 0.0079 | 0.0081 | 0.0157 | 0.0164 | 0.0049 | 0.0222 | 0.0158 | 0.0103 | 0.0206 | 0.0162 | 0.0023 | 0.0083 | 0.0177 |
Nucleolus | 0.0016 | 0.0029 | 0.0027 | 0.0013 | 0.0023 | 0.0021 | 0.0021 | 0.0006 | 0.001 | 0.0008 | 0.0015 | 0.0011 | 0.0017 | 0.0009 | 0.0012 | 0.0014 | 0.0017 | 0.0019 |
Nucleus | 0.0138 | 0.0111 | 0.0048 | 0.0034 | 0.0095 | 0.0056 | 0.0037 | 0.0047 | 0.0051 | 0.0038 | 0.0036 | 0.0054 | 0.0098 | 0.0069 | 0.0029 | 0.0019 | 0.0026 | 0.0069 |
Peroxisomes | 0.0095 | 0.0003 | 0.0083 | 0.0002 | 0.001 | 0.0028 | 0.0033 | 0.0003 | 0.0004 | 0.0002 | 0.0052 | 0.0006 | 0.0005 | 0.0001 | 0.0074 | 0.0002 | 0.0244 | 0.0005 |
Punctate Nuclear | 0.0011 | 0.0003 | 0.001 | 0.0005 | 0.0071 | 0.0009 | 0.0008 | 0.0002 | 0.0025 | 0.0001 | 0.01 | 0.0004 | 0.0003 | 0.0002 | 0.0013 | 0.0001 | 0.0005 | 0.0001 |
Vacuole | 0.3985 | 0.5686 | 0.3847 | 0.6551 | 0.5404 | 0.6302 | 0.4904 | 0.6039 | 0.4684 | 0.6255 | 0.5862 | 0.667 | 0.4739 | 0.6233 | 0.4121 | 0.5659 | 0.582 | 0.6447 |
Vacuole Periphery | 0.0538 | 0.0592 | 0.0568 | 0.0865 | 0.0562 | 0.0797 | 0.0717 | 0.085 | 0.0679 | 0.111 | 0.0519 | 0.0875 | 0.0711 | 0.0781 | 0.0703 | 0.0576 | 0.081 | 0.1055 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 120.419 | 120.5181 | 92.0344 | 87.2542 | 117.9449 | 81.107 | 97.6029 | 83.8414 | 64.6048 | 103.7554 |
Translational Efficiency | 1.0901 | 1.2018 | 0.9326 | 1.0508 | 1.0594 | 1.4763 | 0.9886 | 0.9418 | 1.114 | 0.8948 |
Dataset | Hit |
---|---|
Protein Concentration | ✔ |
Protein Localization | ✘ |
Gene Expression | ✔ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
2280 | 1607 | 1189 | 1024 | 2441 | 1771 | 1264 | 1979 | 4721 | 3378 | 2453 | 3003 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 846.35 | 957.52 | 952.26 | 1331.36 | 917.13 | 857.35 | 1222.30 | 1481.97 | 882.95 | 905.00 | 1091.41 | 1430.61 |
Standard Deviation | 116.65 | 145.62 | 140.55 | 209.92 | 127.88 | 134.31 | 158.39 | 238.07 | 127.58 | 148.48 | 201.78 | 239.74 |
Intensity Change Log 2 | — | 0.178048 | 0.170101 | 0.653574 | — | -0.097242 | 0.414400 | 0.692318 | — | 0.041441 | 0.302298 | 0.673854 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.000088 | 0.000249 | 0.000196 | 0.003158 | 0.000046 | 0.000192 | 0.001125 | 0.002765 | 0.000066 | 0.000219 | 0.000675 | 0.002899 |
Bud Neck | 0.007874 | 0.003700 | 0.009284 | 0.006155 | 0.004028 | 0.004897 | 0.002641 | 0.002509 | 0.005886 | 0.004327 | 0.005861 | 0.003752 |
Bud Site | 0.007496 | 0.007825 | 0.007805 | 0.030762 | 0.004919 | 0.008685 | 0.005315 | 0.013332 | 0.006164 | 0.008276 | 0.006522 | 0.019276 |
Cell Periphery | 0.000411 | 0.000495 | 0.000518 | 0.003666 | 0.000500 | 0.001350 | 0.001577 | 0.009808 | 0.000457 | 0.000943 | 0.001063 | 0.007713 |
Cytoplasm | 0.039985 | 0.018749 | 0.261197* | 0.088199 | 0.021259 | 0.023535 | 0.081329 | 0.101402* | 0.030303 | 0.021258 | 0.168513* | 0.096900 |
Cytoplasmic Foci | 0.042490 | 0.043503 | 0.058220 | 0.035212 | 0.018023 | 0.034685 | 0.014477 | 0.015228 | 0.029839 | 0.038880 | 0.035680 | 0.022042 |
Eisosomes | 0.000037 | 0.000025 | 0.000087 | 0.000167 | 0.000010 | 0.000025 | 0.000149 | 0.000422 | 0.000023 | 0.000025 | 0.000119 | 0.000335 |
Endoplasmic Reticulum | 0.011926 | 0.007169 | 0.065633 | 0.498418* | 0.006614 | 0.009097 | 0.680063* | 0.691477* | 0.009179 | 0.008180 | 0.382241* | 0.625645* |
Endosome | 0.097299 | 0.100151 | 0.045897 | 0.045072 | 0.055457 | 0.099888 | 0.036400 | 0.019201 | 0.075665 | 0.100013 | 0.041003 | 0.028023 |
Golgi | 0.004401 | 0.009442 | 0.000647 | 0.013682 | 0.002353 | 0.007571 | 0.005663 | 0.013772 | 0.003342 | 0.008461 | 0.003232 | 0.013741 |
Lipid Particles | 0.004683 | 0.002078 | 0.017483 | 0.073256 | 0.001168 | 0.002676 | 0.035481 | 0.075111 | 0.002866 | 0.002392 | 0.026757 | 0.074479 |
Mitochondria | 0.002986 | 0.003016 | 0.000691 | 0.000611 | 0.001523 | 0.005736 | 0.000454 | 0.001918 | 0.002230 | 0.004442 | 0.000569 | 0.001472 |
Mitotic Spindle | 0.000447 | 0.000376 | 0.002456 | 0.014828 | 0.000355 | 0.000703 | 0.001022 | 0.000869 | 0.000400 | 0.000547 | 0.001717 | 0.005629 |
None | 0.000122 | 0.000033 | 0.005548 | 0.000761 | 0.000094 | 0.000128 | 0.000501 | 0.003273 | 0.000108 | 0.000083 | 0.002947 | 0.002417 |
Nuclear Periphery | 0.000396 | 0.000131 | 0.014981 | 0.002001 | 0.000085 | 0.000178 | 0.001213 | 0.000241 | 0.000235 | 0.000156 | 0.007887 | 0.000841 |
Nuclear Periphery Foci | 0.000207 | 0.000298 | 0.028530 | 0.043747 | 0.000116 | 0.000313 | 0.022476 | 0.007114 | 0.000160 | 0.000305 | 0.025410 | 0.019606 |
Nucleolus | 0.000324 | 0.000215 | 0.002078 | 0.000254 | 0.000180 | 0.000351 | 0.000071 | 0.000069 | 0.000250 | 0.000286 | 0.001043 | 0.000132 |
Nucleus | 0.004146 | 0.001275 | 0.122659 | 0.005153 | 0.001115 | 0.001779 | 0.004212 | 0.002125 | 0.002579 | 0.001539 | 0.061625 | 0.003157 |
Peroxisomes | 0.001055 | 0.001210 | 0.001061 | 0.000743 | 0.000379 | 0.001564 | 0.000262 | 0.001304 | 0.000705 | 0.001396 | 0.000649 | 0.001112 |
Vacuole | 0.745452* | 0.759166* | 0.351116* | 0.130481 | 0.850947* | 0.760208* | 0.103696 | 0.035694 | 0.799998* | 0.759712* | 0.223624* | 0.068015 |
Vacuole Periphery | 0.028176 | 0.040894 | 0.003915 | 0.003674 | 0.030828 | 0.036441 | 0.001873 | 0.002367 | 0.029547 | 0.038559 | 0.002863 | 0.002813 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | -1.42 | -3.49 | -6.80 | -6.27 | -6.52 | -2.84 | -5.34 | -4.99 | -4.70 | -2.52 | -2.52 | -5.78 | -7.27 | -6.80 | -5.26 |
Bud Neck | 6.21 | -1.68 | 1.17 | -4.22 | 2.52 | -1.30 | 2.03 | 2.81 | 3.83 | 0.83 | 3.34 | -0.49 | 4.02 | 0.61 | 3.89 |
Bud Site | -0.37 | -0.46 | -7.75 | -7.67 | -7.27 | -4.24 | -1.76 | -6.09 | -3.26 | -4.68 | -3.36 | -1.40 | -9.50 | -7.92 | -8.23 |
Cell Periphery | -1.10 | -0.68 | -6.32 | -6.11 | -5.93 | -2.32 | -7.91 | -13.14 | -10.63 | -11.37 | -2.49 | -6.27 | -14.45 | -12.57 | -12.98 |
Cytoplasm | 7.90 | -28.38 | -9.46 | -13.72 | 18.37 | -0.84 | -14.99 | -20.14 | -18.84 | -3.68 | 4.88 | -29.67 | -20.94 | -23.87 | 12.81 |
Cytoplasmic Foci | -0.26 | -4.94 | 2.13 | 2.20 | 6.73 | -6.79 | 1.83 | 1.67 | 8.09 | -0.24 | -4.43 | -3.21 | 4.62 | 8.39 | 7.70 |
Eisosomes | 1.57 | -6.55 | -7.58 | -8.08 | -4.57 | -4.45 | -6.27 | -8.09 | -7.77 | -4.85 | -0.48 | -7.92 | -9.10 | -9.02 | -5.92 |
Endoplasmic Reticulum | 4.87 | -13.36 | -36.48 | -36.90* | -31.06* | -3.15 | -71.32* | -90.09* | -89.54* | -1.59 | 1.59 | -45.89 | -88.40 | -88.55* | -22.70 |
Endosome | -0.57 | 14.30 | 14.50 | 12.56 | 0.09 | -9.67 | 6.64 | 15.32 | 19.52 | 8.58 | -7.22 | 15.09 | 22.94 | 24.30 | 7.59 |
Golgi | -3.94 | 6.53 | -5.81 | -1.98 | -9.06 | -5.29 | -5.39 | -10.18 | -4.56 | -7.36 | -6.40 | 0.32 | -11.33 | -4.60 | -12.12 |
Lipid Particles | 5.33 | -11.14 | -15.14 | -15.76 | -12.10 | -4.42 | -13.64 | -20.56 | -20.05 | -8.74 | 1.48 | -16.73 | -25.30 | -25.46 | -14.97 |
Mitochondria | -0.04 | 5.44 | 5.53 | 5.22 | 0.60 | -4.42 | 3.44 | -0.85 | 3.98 | -4.32 | -3.81 | 6.40 | 2.24 | 5.26 | -4.04 |
Mitotic Spindle | 0.16 | -3.56 | -6.40 | -6.43 | -5.10 | -0.87 | -1.36 | -1.33 | -0.59 | 0.23 | -0.55 | -3.68 | -6.41 | -6.25 | -4.13 |
None | 2.63 | -3.77 | -2.39 | -2.72 | 3.19 | -0.55 | -2.13 | -4.37 | -4.33 | -3.70 | 0.66 | -4.03 | -4.76 | -4.82 | 0.49 |
Nuclear Periphery | 1.52 | -11.73 | -4.94 | -6.59 | 10.16 | -2.87 | -9.72 | -10.65 | -2.07 | 8.36 | 0.94 | -12.36 | -4.84 | -6.90 | 11.26 |
Nuclear Periphery Foci | -1.62 | -14.39 | -13.94 | -13.91 | -4.16 | -2.69 | -13.53 | -11.56 | -11.17 | 8.82 | -3.11 | -19.73 | -16.43 | -16.29 | 3.34 |
Nucleolus | 2.63 | -9.33 | 0.57 | -0.57 | 8.71 | -1.85 | 7.09 | 7.97 | 2.95 | 0.46 | -0.78 | -8.49 | 3.09 | 2.48 | 9.31 |
Nucleus | 4.44 | -22.27 | -2.22 | -6.05 | 21.72 | -1.81 | -6.54 | -4.88 | -1.38 | 4.28 | 2.81 | -20.83 | -2.64 | -5.76 | 20.41 |
Peroxisomes | -0.86 | 0.02 | 2.08 | 2.49 | 2.37 | -5.83 | 2.03 | -7.93 | 1.29 | -9.27 | -4.90 | 0.81 | -4.01 | 2.04 | -5.04 |
Vacuole | -1.55 | 39.84* | 66.44* | 62.80* | 19.51 | 11.13 | 108.40* | 166.75* | 97.45* | 12.13 | 6.61 | 88.54* | 154.20* | 118.96* | 24.84 |
Vacuole Periphery | -7.19 | 26.42* | 24.24* | 23.26* | 0.42 | -3.15 | 27.47 | 26.39* | 23.32 | -1.59 | -7.18 | 37.96 | 36.58 | 33.28* | 0.06 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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