Standard name | |
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Human Ortholog | |
Description | Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | – | – | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3 | 1 |
Bud | – | – | 0 | 0 | 0 | 3 | 0 | – | 5 | 2 | 11 | 3 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 4 | 11 | 22 |
Bud Neck | – | – | 0 | 2 | 8 | 1 | 15 | – | 7 | 12 | 11 | 5 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 4 | 8 |
Bud Site | – | – | 0 | 0 | 0 | 2 | 0 | – | 2 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | – | – | 0 | 0 | 1 | 2 | 4 | – | 2 | 1 | 3 | 3 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 |
Cytoplasm | – | – | 12 | 151 | 305 | 284 | 533 | – | 311 | 323 | 316 | 391 | 96 | 269 | 290 | 24 | 13 | 4 | 9 | 5 | 33 | 72 | 163 | 180 |
Endoplasmic Reticulum | – | – | 0 | 0 | 2 | 0 | 1 | – | 0 | 1 | 0 | 1 | 0 | 0 | 1 | 4 | 0 | 2 | 0 | 0 | 1 | 1 | 4 | 7 |
Endosome | – | – | 0 | 0 | 9 | 6 | 23 | – | 8 | 6 | 4 | 11 | 17 | 68 | 54 | 5 | 0 | 1 | 0 | 0 | 0 | 8 | 11 | 14 |
Golgi | – | – | 0 | 2 | 1 | 0 | 2 | – | 0 | 2 | 1 | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 |
Mitochondria | – | – | 0 | 3 | 1 | 45 | 22 | – | 66 | 49 | 120 | 99 | 0 | 0 | 1 | 5 | 4 | 5 | 1 | 2 | 3 | 6 | 20 | 19 |
Nucleus | – | – | 4 | 8 | 21 | 14 | 46 | – | 21 | 18 | 24 | 25 | 51 | 77 | 91 | 90 | 63 | 36 | 2 | 11 | 9 | 47 | 119 | 99 |
Nuclear Periphery | – | – | 0 | 0 | 0 | 0 | 1 | – | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 1 | 1 |
Nucleolus | – | – | 0 | 0 | 0 | 0 | 0 | – | 0 | 1 | 0 | 2 | 1 | 5 | 1 | 4 | 1 | 4 | 1 | 0 | 2 | 8 | 33 | 28 |
Peroxisomes | – | – | 0 | 0 | 1 | 0 | 0 | – | 0 | 1 | 3 | 1 | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 |
SpindlePole | – | – | 0 | 1 | 7 | 1 | 15 | – | 4 | 8 | 7 | 7 | 3 | 6 | 11 | 2 | 1 | 1 | 0 | 1 | 1 | 11 | 43 | 52 |
Vac/Vac Membrane | – | – | 0 | 8 | 24 | 5 | 38 | – | 17 | 19 | 18 | 26 | 21 | 66 | 82 | 13 | 4 | 5 | 3 | 5 | 4 | 27 | 51 | 58 |
Unique Cell Count | 16 | 171 | 354 | 339 | 652 | 396 | 405 | 446 | 516 | 181 | 456 | 482 | 134 | 83 | 52 | 22 | 30 | 59 | 200 | 478 | 510 | |||
Labelled Cell Count | 16 | 175 | 380 | 363 | 700 | 444 | 444 | 518 | 574 | 190 | 494 | 531 | 149 | 86 | 59 | 22 | 30 | 59 | 200 | 478 | 510 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | – | – | 4.4 | 4.5 | 5.3 | 3.7 | 4.4 | – | 3.8 | 4.2 | 3.8 | 4.0 | 5.2 | 5.6 | 5.6 | 7.7 | 7.7 | 7.6 | 4.6 | 4.8 | 4.8 |
Std Deviation (1e-4) | – | – | 0.6 | 1.3 | 1.6 | 1.2 | 1.3 | – | 1.0 | 1.5 | 1.3 | 1.1 | 1.1 | 1.7 | 1.7 | 2.0 | 1.7 | 1.5 | 1.5 | 1.7 | 1.5 |
Intensity Change (Log2) | – | – | – | 0.02 | 0.26 | -0.28 | -0.01 | – | -0.24 | -0.09 | -0.21 | -0.16 | 0.24 | 0.33 | 0.33 | 0.79 | 0.79 | 0.77 | 0.07 | 0.12 | 0.11 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 1.5 | 1.2 | 0.9 | 0.7 | – | 0.3 | 0.5 | -0.4 | 0.1 | -1.7 | -1.3 | -1.2 | 0 | 0 | 0 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3.3 | 4.0 | 3.1 |
Nuclear Periphery | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | -0.4195 | -0.9396 | -1.0894 | -0.8176 | -1.5468 | -1.367 | -0.5456 | -0.3709 | -0.1755 | -0.5257 | -0.5366 | -0.2004 | 0.6196 | 1.2949 | 0.9221 | 1.2254 | 0.6284 | 0.8627 |
Actin | 0.0808 | 0.001 | 0.011 | 0.0001 | 0.0007 | 0.0068 | 0.0575 | 0.0024 | 0.0026 | 0.0013 | 0 | 0.0204 | 0.0279 | 0.0001 | 0.0005 | 0.0003 | 0.0005 | 0.0018 |
Bud | 0.0013 | 0.0023 | 0.0003 | 0 | 0.0001 | 0.0004 | 0.0005 | 0.0008 | 0.0056 | 0.0026 | 0.0001 | 0.0002 | 0.0004 | 0.0002 | 0.0007 | 0.0001 | 0.0001 | 0.0003 |
Bud Neck | 0.0053 | 0.0006 | 0.0011 | 0.0007 | 0.0026 | 0.0036 | 0.001 | 0.0015 | 0.0015 | 0.005 | 0.0005 | 0.0053 | 0.0029 | 0.003 | 0.0016 | 0.0006 | 0.0053 | 0.0053 |
Bud Periphery | 0.0021 | 0.0014 | 0.0005 | 0 | 0.0001 | 0.0006 | 0.0011 | 0.0009 | 0.0064 | 0.0018 | 0.0001 | 0.0003 | 0.0005 | 0.0002 | 0.0006 | 0 | 0.0002 | 0.0004 |
Bud Site | 0.004 | 0.0153 | 0.0031 | 0.0001 | 0.0003 | 0.0005 | 0.0022 | 0.0063 | 0.0077 | 0.0021 | 0.0002 | 0.0003 | 0.0024 | 0.0014 | 0.0105 | 0.0001 | 0.0006 | 0.0008 |
Cell Periphery | 0.0004 | 0.0003 | 0.0004 | 0 | 0 | 0.0001 | 0.0005 | 0.0002 | 0.0005 | 0.0006 | 0 | 0.0001 | 0.0003 | 0.0001 | 0.0001 | 0 | 0.0001 | 0.0001 |
Cytoplasm | 0.0634 | 0.048 | 0.053 | 0.0129 | 0.1048 | 0.095 | 0.0411 | 0.0749 | 0.0405 | 0.0552 | 0.0134 | 0.0958 | 0.0891 | 0.062 | 0.0655 | 0.0994 | 0.0437 | 0.0802 |
Cytoplasmic Foci | 0.0279 | 0.0049 | 0.0069 | 0.0003 | 0.0432 | 0.0167 | 0.0204 | 0.0176 | 0.0302 | 0.0298 | 0.0437 | 0.008 | 0.0104 | 0.0038 | 0.0109 | 0.0052 | 0.0033 | 0.0049 |
Eisosomes | 0.0014 | 0.0001 | 0.0003 | 0 | 0.0001 | 0.0001 | 0.0006 | 0.0001 | 0.0001 | 0.0001 | 0 | 0.0001 | 0.0005 | 0.0001 | 0.0001 | 0 | 0 | 0.0001 |
Endoplasmic Reticulum | 0.0114 | 0.0086 | 0.0027 | 0.0002 | 0.0018 | 0.0024 | 0.0162 | 0.0033 | 0.0034 | 0.0014 | 0.0001 | 0.0011 | 0.0053 | 0.0028 | 0.0015 | 0.0008 | 0.0012 | 0.0009 |
Endosome | 0.0429 | 0.0064 | 0.0091 | 0.0004 | 0.0182 | 0.0176 | 0.0209 | 0.0134 | 0.0124 | 0.0094 | 0.0016 | 0.004 | 0.0203 | 0.0062 | 0.006 | 0.0015 | 0.0017 | 0.0046 |
Golgi | 0.0105 | 0.0002 | 0.0025 | 0 | 0.0064 | 0.0079 | 0.0092 | 0.0036 | 0.0039 | 0.0025 | 0.0002 | 0.0079 | 0.0042 | 0.0001 | 0.0005 | 0.0001 | 0.0001 | 0.0018 |
Lipid Particles | 0.0158 | 0.0009 | 0.0033 | 0 | 0.0244 | 0.0027 | 0.0166 | 0.0035 | 0.0071 | 0.009 | 0.0005 | 0.0024 | 0.0141 | 0.0015 | 0.003 | 0.0001 | 0.0002 | 0.006 |
Mitochondria | 0.0085 | 0.0004 | 0.0042 | 0.0003 | 0.0013 | 0.0207 | 0.0179 | 0.0011 | 0.003 | 0.0108 | 0.0009 | 0.0016 | 0.0024 | 0.0004 | 0.0008 | 0.0003 | 0.0005 | 0.0031 |
None | 0.1818 | 0.2007 | 0.2143 | 0.1143 | 0.0868 | 0.2103 | 0.1496 | 0.252 | 0.2076 | 0.219 | 0.2716 | 0.147 | 0.2807 | 0.3218 | 0.3544 | 0.1968 | 0.3679 | 0.267 |
Nuclear Periphery | 0.0275 | 0.0382 | 0.0221 | 0.0044 | 0.0215 | 0.0217 | 0.0216 | 0.0125 | 0.0107 | 0.0176 | 0.0005 | 0.0073 | 0.013 | 0.0037 | 0.0087 | 0.0024 | 0.0062 | 0.0045 |
Nucleolus | 0.0164 | 0.0169 | 0.0145 | 0.0067 | 0.0238 | 0.021 | 0.0105 | 0.0173 | 0.0127 | 0.0236 | 0.0633 | 0.0417 | 0.0109 | 0.0287 | 0.01 | 0.0062 | 0.0434 | 0.0285 |
Nucleus | 0.4133 | 0.6203 | 0.5736 | 0.7959 | 0.5154 | 0.5021 | 0.5588 | 0.533 | 0.5737 | 0.5287 | 0.575 | 0.5935 | 0.4575 | 0.543 | 0.4643 | 0.6719 | 0.3662 | 0.5273 |
Peroxisomes | 0.0137 | 0.0001 | 0.0009 | 0 | 0.0087 | 0.0022 | 0.0141 | 0.0054 | 0.0165 | 0.006 | 0.0009 | 0.0084 | 0.0052 | 0.0001 | 0.0023 | 0.0001 | 0.0001 | 0.0007 |
Punctate Nuclear | 0.0638 | 0.0295 | 0.0725 | 0.0635 | 0.1379 | 0.0644 | 0.0349 | 0.0449 | 0.0516 | 0.0685 | 0.0269 | 0.0539 | 0.0481 | 0.0169 | 0.057 | 0.0134 | 0.1552 | 0.0601 |
Vacuole | 0.0056 | 0.0032 | 0.003 | 0.0002 | 0.0013 | 0.0022 | 0.0026 | 0.0045 | 0.0017 | 0.0038 | 0.0004 | 0.0006 | 0.0031 | 0.0036 | 0.001 | 0.0004 | 0.0032 | 0.0012 |
Vacuole Periphery | 0.0023 | 0.0006 | 0.0007 | 0 | 0.0005 | 0.001 | 0.0021 | 0.0005 | 0.0006 | 0.0011 | 0.0001 | 0.0002 | 0.0008 | 0.0002 | 0.0002 | 0.0001 | 0.0003 | 0.0004 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 8.2099 | 16.146 | 15.2486 | 16.3743 | 10.6444 | 9.8464 | 15.2122 | 23.5394 | 12.2874 | 18.5066 |
Translational Efficiency | 2.3659 | 1.1135 | 1.188 | 1.0672 | 1.7742 | 1.9194 | 1.2618 | 0.9939 | 2.1569 | 1.288 |
Dataset | Hit |
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Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✔ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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