Standard name
Human Ortholog
Description Subunit (90 kDa) of TFIID and SAGA complexes; involved in RNA polymerase II transcription initiation and in chromatin modification
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0.06 0 0.05 0 0 0 0 0 0 0 – 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0
Cytoplasm 0.91 0.93 0.98 0.98 0.99 0.95 0.95 0.95 0.85 0.78 0.8 0.89 0.99 0.99 0.99 0.98 0.98 – 0.75 0.74 0.7 0.68 0.67 0.71
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.1 0.05 – 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0
Mitochondria 0.14 0.09 0 0 0 0 0.09 0.08 0.26 0.31 0.31 0.14 0.05 0 0 0.05 0 – 0.11 0.11 0.13 0.11 0.14 0.09
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0.07
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2 – 1 3 4 2 6 2
Bud 0 2 0 0 0 0 3 4 2 5 9 4 0 1 0 0 0 – 1 0 1 3 4 5
Bud Neck 0 1 5 2 7 5 14 18 2 9 9 12 0 0 0 3 11 – 0 1 1 2 2 4
Bud Site 0 0 0 0 0 0 0 0 0 0 3 1 0 0 0 0 0 – – – – – – –
Cell Periphery 0 0 2 3 3 0 0 1 0 0 0 1 1 1 0 2 4 – 1 1 3 3 1 2
Cytoplasm 124 199 271 199 340 200 316 290 152 139 199 255 161 269 254 492 518 – 110 149 164 96 161 182
Endoplasmic Reticulum 1 2 0 0 0 0 0 0 1 0 0 0 0 1 1 49 25 – 2 0 0 2 1 6
Endosome 0 0 0 1 0 0 0 0 0 0 0 0 0 0 1 1 4 – 4 4 1 2 1 0
Golgi 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 2 2 – 0 0 0 1 2 0
Mitochondria 19 19 1 3 2 9 30 25 46 55 77 39 8 1 0 26 9 – 16 21 30 15 34 23
Nucleus 0 1 1 2 2 0 2 4 3 3 1 1 2 1 0 3 2 – 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 1 0 1 0 0 0 0 0 0 0 0 0 – 0 0 1 0 0 0
Peroxisomes 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 1 – 0 0 0 0 0 1
SpindlePole 0 0 2 0 1 3 7 4 3 4 9 10 0 0 0 0 0 – 0 3 1 1 4 2
Vac/Vac Membrane 0 0 1 0 0 0 0 2 0 0 0 2 0 1 1 3 4 – 2 7 4 5 8 18
Unique Cell Count 137 213 277 204 345 210 333 306 178 179 250 285 163 271 256 502 530 147 203 234 142 239 257
Labelled Cell Count 144 224 284 210 355 218 372 350 209 215 307 325 172 275 257 581 582 147 203 234 142 239 257


Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 3.3 2.9 4.2 3.9 4.6 3.5 3.4 3.8 3.2 2.9 2.7 3.5 3.6 3.8 4.1 5.1 5.1 4.8 3.5 3.5 4.1
Std Deviation (1e-4) 0.4 0.5 0.9 1.4 1.8 1.2 1.0 1.1 1.4 0.7 0.6 1.6 0.7 1.3 1.7 1.2 1.0 0.8 1.4 1.3 2.0
Intensity Change (Log2) – – – -0.1 0.14 -0.28 -0.31 -0.14 -0.41 -0.54 -0.62 -0.26 -0.21 -0.13 -0.04 0.29 0.27 0.2 -0.25 -0.28 -0.02


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – –
Bud Neck – – – – – – – – – – – – – – –
Bud Site – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – –
Endosome – – – – – – – – – – – – – – –
Golgi – – – – – – – – – – – – – – –
Mitochondria – – – – – – – – – – – – – – –
Nuclear Periphery – – – – – – – – – – – – – – –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – –
Peroxisomes – – – – – – – – – – – – – – –
SpindlePole – – – – – – – – – – – – – – –
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – – –
Cytoplasm – – – – – – – – – – – – – – –
Nucleus – – – – – – – – – – – – – – –
Vacuole – – – – – – – – – – – – – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 1.422 1.3575 1.1996 0.9831 1.5335 1.3046 -0.9791 -1.2025 -1.2583 -1.3054 -0.4186 -1.3569 -1.8873 -1.5584 -1.4515 -1.144 -0.4259 -1.5389
Actin 0.0279 0.0002 0.0022 0.0022 0.0593 0.0015 0.0302 0.0014 0.0065 0.0005 0.0119 0.0114 0.0468 0 0.0108 0 0.1196 0.006
Bud 0.0006 0.0006 0.0005 0.0009 0.0007 0.0004 0.0013 0.0061 0.0004 0.0002 0.0052 0.0008 0.0003 0 0.0001 0 0.0015 0.0004
Bud Neck 0.0011 0.0002 0.0006 0.0002 0.0013 0.0005 0.0007 0.001 0.0003 0.0017 0.0032 0.0003 0.0003 0 0.0002 0 0.0072 0.0015
Bud Periphery 0.0017 0.0015 0.0017 0.0033 0.0016 0.0014 0.0032 0.0082 0.0007 0.0001 0.0052 0.0049 0.0004 0 0.0003 0 0.0031 0.0009
Bud Site 0.0038 0.0025 0.0052 0.0004 0.0022 0.0003 0.0029 0.027 0.0015 0.002 0.0362 0.0003 0.0014 0 0.0005 0 0.0044 0.0012
Cell Periphery 0.0003 0.0001 0.0001 0.0001 0.0002 0.0001 0.0005 0.0008 0.0001 0.0001 0.0005 0.0002 0.0003 0 0.0001 0 0.0004 0.0001
Cytoplasm 0.0219 0.0162 0.0222 0.0248 0.0277 0.0347 0.0302 0.0286 0.0378 0.0409 0.0325 0.0521 0.0082 0.0325 0.0409 0.0072 0.029 0.0766
Cytoplasmic Foci 0.0171 0.0022 0.013 0.0065 0.0344 0.0073 0.0312 0.0182 0.0168 0.0217 0.0213 0.0141 0.0077 0.0015 0.0045 0.0008 0.0058 0.005
Eisosomes 0.0005 0.0002 0.0002 0.0001 0.001 0.0001 0.0006 0.0002 0.0002 0.0001 0.0002 0.0002 0.0016 0.0002 0.0003 0.0001 0.0008 0.0001
Endoplasmic Reticulum 0.0021 0.0002 0.0009 0.0002 0.0032 0.001 0.0031 0.0004 0.0017 0.0015 0.0023 0.0013 0.0017 0.0006 0.0044 0.0001 0.0114 0.0008
Endosome 0.0294 0.002 0.0069 0.024 0.0261 0.0029 0.038 0.0047 0.0171 0.0237 0.0624 0.0071 0.0071 0.0002 0.0034 0 0.0209 0.0023
Golgi 0.0089 0.0004 0.0038 0.0315 0.0134 0.0013 0.0168 0.0004 0.005 0.0127 0.0222 0.0022 0.0035 0 0.0048 0 0.0105 0.0009
Lipid Particles 0.0249 0.0018 0.0034 0.0026 0.0455 0.0048 0.0183 0.0014 0.0053 0.0144 0.0499 0.003 0.0038 0.0002 0.0049 0.0001 0.0104 0.0005
Mitochondria 0.0145 0.0011 0.0038 0.0048 0.0715 0.0075 0.0074 0.0006 0.0052 0.0217 0.0175 0.008 0.0013 0 0.0013 0 0.0034 0.0027
None 0.7955 0.9656 0.9174 0.8949 0.6558 0.9218 0.7741 0.8973 0.891 0.8515 0.6549 0.8808 0.9053 0.964 0.9052 0.9913 0.6846 0.8979
Nuclear Periphery 0.0096 0.0003 0.0035 0.0002 0.01 0.0014 0.0066 0.0002 0.0008 0.0009 0.0131 0.0052 0.0017 0.0001 0.0072 0.0001 0.021 0.0004
Nucleolus 0.0024 0.0012 0.0005 0.0001 0.0014 0.002 0.0009 0.0005 0.0002 0.0002 0.0033 0.0002 0.0004 0 0.0001 0 0.0029 0.0002
Nucleus 0.0061 0.0008 0.003 0.0001 0.0044 0.0013 0.0062 0.0006 0.0004 0.0007 0.012 0.0008 0.0005 0.0001 0.0013 0 0.0374 0.0005
Peroxisomes 0.0178 0.0003 0.0071 0.0015 0.025 0.0016 0.0096 0.0005 0.0055 0.0009 0.0187 0.0041 0.0051 0 0.0077 0 0.0041 0.0003
Punctate Nuclear 0.0099 0.0018 0.0018 0.0002 0.0117 0.0074 0.0078 0.0006 0.0011 0.0008 0.0099 0.002 0.0019 0.0001 0.0003 0.0001 0.0186 0.0004
Vacuole 0.0023 0.0006 0.0017 0.0007 0.002 0.0004 0.0087 0.0013 0.0015 0.0022 0.0147 0.0008 0.0005 0.0001 0.0009 0 0.0022 0.0011
Vacuole Periphery 0.0016 0.0001 0.0005 0.0007 0.0016 0.0002 0.0018 0.0001 0.0006 0.0016 0.003 0.0007 0.0003 0 0.0007 0 0.0011 0.0002

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 21.8655 20.5752 26.8712 32.32 25.067 21.6234 27.7728 29.3457 27.6315 31.4237
Translational Efficiency 1.3783 1.6802 1.2345 1.021 1.2209 1.3084 1.143 0.84 1.063 1.0503

Hit Data

Dataset Hit
Protein Concentration ✔
Protein Localization ✘
Gene Expression ✔
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1765 1481 278 1320 1184 1354 90 437 2949 2835 368 1757

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 627.40 730.27 928.20 794.09 661.27 705.89 901.83 815.39 641.00 718.63 921.75 799.39
Standard Deviation 70.29 93.44 108.16 110.12 72.18 91.32 87.81 101.37 72.97 93.23 104.17 108.40
Intensity Change Log 2 — 0.219044 0.565050 0.339917 — 0.094204 0.447616 0.302251 — 0.156334 0.505984 0.320712

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000103 0.000278 0.000973 0.000580 0.000081 0.000490 0.000509 0.000315 0.000094 0.000379 0.000859 0.000514
Bud Neck 0.030291 0.026527 0.007057 0.010323 0.009984 0.060751 0.003092 0.004442 0.022138 0.042872 0.006087 0.008860
Bud Site 0.005067 0.006327 0.007144 0.045294 0.002279 0.019020 0.002506 0.019023 0.003948 0.012389 0.006009 0.038760
Cell Periphery 0.000253 0.000138 0.000221 0.000200 0.000120 0.000259 0.000311 0.000140 0.000200 0.000196 0.000243 0.000185
Cytoplasm 0.482804* 0.439400* 0.060587 0.432994* 0.488060* 0.348585* 0.242742* 0.448970* 0.484914* 0.396027* 0.105136* 0.436967*
Cytoplasmic Foci 0.218233* 0.206036* 0.002399 0.023815 0.166513 0.256392* 0.017954 0.032647 0.197468* 0.230086* 0.006203 0.026012
Eisosomes 0.000367 0.000122 0.000074 0.000050 0.000104 0.000225 0.000137 0.000060 0.000262 0.000171 0.000089 0.000052
Endoplasmic Reticulum 0.000613 0.000706 0.012312 0.001904 0.000453 0.000698 0.008570 0.002363 0.000549 0.000702 0.011397 0.002018
Endosome 0.012590 0.015164 0.002449 0.006017 0.005652 0.036422 0.004932 0.005500 0.009804 0.025316 0.003056 0.005888
Golgi 0.002483 0.005276 0.000110 0.001702 0.001133 0.007857 0.000111 0.001016 0.001941 0.006509 0.000110 0.001531
Lipid Particles 0.009966 0.006154 0.002073 0.001330 0.004529 0.011514 0.004587 0.001611 0.007783 0.008714 0.002687 0.001400
Mitochondria 0.003747 0.008759 0.000960 0.005209 0.001879 0.020201 0.000743 0.001690 0.002997 0.014224 0.000906 0.004334
Mitotic Spindle 0.000608 0.005566 0.000824 0.046290 0.000520 0.010970 0.000844 0.022014 0.000573 0.008147 0.000829 0.040252
None 0.004820 0.004019 0.003426 0.006261 0.004330 0.002855 0.010933 0.004396 0.004623 0.003463 0.005262 0.005797
Nuclear Periphery 0.000299 0.000502 0.002278 0.001919 0.000434 0.000513 0.003314 0.002070 0.000354 0.000507 0.002532 0.001956
Nuclear Periphery Foci 0.000197 0.000336 0.001338 0.001056 0.000244 0.000285 0.001877 0.005769 0.000216 0.000312 0.001470 0.002228
Nucleolus 0.001031 0.001626 0.001153 0.000771 0.001029 0.002355 0.001875 0.000380 0.001030 0.001975 0.001329 0.000674
Nucleus 0.164474* 0.207135* 0.849039* 0.315140* 0.279169* 0.112029 0.583762* 0.318803* 0.210523* 0.161712* 0.784161* 0.316051*
Peroxisomes 0.005307 0.016563 0.000284 0.001029 0.004641 0.023069 0.000523 0.000970 0.005040 0.019670 0.000343 0.001015
Vacuole 0.055978 0.047928 0.045076 0.093399 0.028420 0.082065 0.110405 0.126667 0.044914 0.064232 0.061054 0.101673
Vacuole Periphery 0.000769 0.001439 0.000223 0.004719 0.000423 0.003445 0.000274 0.001152 0.000630 0.002397 0.000236 0.003832

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -2.93 -11.54 -7.10 -2.44 7.41 -3.59 -5.62 -5.30 1.25 3.19 -4.56 -12.62 -8.43 -0.83 7.96
Bud Neck 1.45 10.73 11.22 8.56 0.62 -13.61 4.10 4.59 15.89 0.14 -8.74 10.56 11.24 17.08 0.65
Bud Site -1.29 -0.73 -7.21 -6.81 -5.86 -9.80 -2.28 -3.24 2.51 -2.64 -8.50 -1.30 -8.08 -4.19 -6.32
Cell Periphery 5.46 0.06 4.44 -1.38 4.12 -3.79 -4.03 0.46 3.84 4.07 0.23 -2.30 3.81 2.73 5.43
Cytoplasm 4.92 52.93 13.60 7.63 -33.00 12.72 13.96 10.30 1.34 -6.19 12.67 46.71 17.01 5.30 -27.78
Cytoplasmic Foci 1.61 46.16 41.53 33.19 -12.20 -11.08 23.80 24.07 36.89 -2.02 -6.04 52.02 47.58 50.25 -9.78
Eisosomes 14.37 14.91 18.74 10.28 4.60 -8.33 -3.05 3.27 11.59 5.31 7.39 11.89 18.52 15.58 6.55
Endoplasmic Reticulum -0.70 -15.74 -12.13 -12.93 13.29 -1.67 -6.98 -7.86 -6.99 5.09 -1.45 -17.10 -15.22 -14.16 13.93
Endosome -1.79 10.09 9.34 9.73 -0.49 -14.73 0.37 2.64 15.42 2.01 -12.21 9.36 9.30 18.10 0.56
Golgi -3.14 8.55 4.25 5.09 -1.95 -7.34 2.95 1.62 8.36 -0.99 -7.20 8.31 3.97 9.14 -2.15
Lipid Particles 4.26 8.93 11.15 10.75 3.24 -6.40 -0.08 7.28 9.60 5.23 -1.33 8.43 12.96 13.31 5.29
Mitochondria -4.06 4.28 0.97 4.59 -2.39 -9.75 2.03 1.09 9.98 -0.43 -10.05 4.67 0.88 10.03 -2.40
Mitotic Spindle -3.85 -2.14 -8.51 -6.77 -7.13 -5.20 -0.58 -3.23 -0.58 -3.18 -6.42 -2.22 -9.12 -5.97 -7.76
None 0.75 1.66 -0.27 -1.28 -2.69 1.45 -0.45 0.96 -0.82 0.91 1.57 0.98 -0.07 -2.09 -1.23
Nuclear Periphery -4.80 -15.90 -13.30 -12.13 1.29 -0.90 -10.75 -5.48 -5.15 3.57 -3.43 -18.93 -14.10 -12.84 2.96
Nuclear Periphery Foci -3.31 -5.19 -9.86 -7.66 2.76 -0.90 -5.84 -1.70 -1.68 -0.22 -3.31 -6.69 -3.44 -3.18 1.51
Nucleolus -3.27 -1.93 4.05 5.20 3.81 -3.40 -0.25 5.36 5.80 2.90 -4.66 -1.78 5.66 7.25 4.65
Nucleus -6.16 -50.47 -23.08 -15.61 31.62 19.46 -10.28 -4.88 -16.68 6.84 8.74 -40.94 -19.19 -25.55 27.61
Peroxisomes -7.23 12.61 11.65 10.67 -1.96 -10.66 5.60 5.33 14.86 -0.42 -12.76 12.05 11.27 17.92 -1.90
Vacuole 2.19 -1.82 -18.21 -19.09 -10.63 -14.26 -7.91 -16.54 -12.04 -1.70 -8.14 -6.73 -25.04 -20.95 -8.91
Vacuole Periphery -3.01 8.33 -1.80 -0.86 -2.52 -6.76 3.14 -0.27 5.71 -0.91 -7.24 8.70 -1.91 1.18 -2.61
Standard name
Human Ortholog
Description Subunit (90 kDa) of TFIID and SAGA complexes; involved in RNA polymerase II transcription initiation and in chromatin modification
Localization
Cell Percentages cytoplasm (27%), vacuole (3%), mixed (41%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Taf5

Taf5


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Taf5-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available