Standard name
Human Ortholog
Description Protein involved in cell separation during budding; one of two S. cerevisiae homologs (Sds23p and Sds24p) of the S. pombe Sds23 protein, which is implicated in APC/cyclosome regulation; may play an indirect role in fluid-phase endocytosis; protein abundance increases in response to DNA replication stress; SDS24 has a paralog, SDS23, that arose from the whole genome duplication
DOWNLOAD

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.95 0.97 0.97 0.98 0.95 0.9 0.79 0.72 0.78 0.71 0.74 0.7 1.0 0.99 1.0 0.97 0.97 0.97 0.94 0.93 0.94 0.92 0.92 0.87
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0.05 0.05 0.1 0.12 0.12 0.18 0.22 0.21 0.2 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0.09 0.17 0.08 0.1 0.13 0.08 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 1 1 0 2 1 1 0 0 0 0 0 0 0 3 0 0 2 0
Bud 1 0 5 2 1 1 2 3 5 4 0 4 0 0 0 0 1 0 0 0 0 0 2 4
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 1 0 0 1 3 5 12 3 6 3 7 1 4 5 3 0 3 0 0 0 0 0 0
Cytoplasm 199 232 243 131 233 283 293 344 204 228 128 144 261 364 442 468 267 174 192 227 241 163 274 199
Endoplasmic Reticulum 3 1 1 0 0 4 3 6 7 10 3 8 0 2 1 12 8 5 1 0 1 1 1 4
Endosome 0 0 0 0 0 0 2 0 0 0 0 0 0 0 0 1 0 1 2 2 1 0 1 1
Golgi 1 1 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 2 0 1 0 1
Mitochondria 8 3 4 7 12 32 45 55 46 72 37 41 0 1 0 8 2 2 1 1 1 2 2 2
Nucleus 4 2 4 0 0 2 4 1 4 4 3 7 1 3 0 5 3 2 0 1 1 0 0 1
Nuclear Periphery 1 2 0 0 0 0 1 0 1 3 0 0 0 0 0 0 0 0 0 0 0 0 1 0
Nucleolus 0 0 1 0 1 0 0 0 1 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0
Vac/Vac Membrane 0 1 2 2 6 8 35 81 22 33 22 17 0 1 1 5 7 2 1 1 4 3 3 5
Unique Cell Count 209 238 250 134 245 315 369 476 262 321 174 207 262 369 444 481 274 179 206 245 257 177 297 229
Labelled Cell Count 217 243 260 142 254 333 391 504 293 362 197 230 263 375 449 502 288 189 206 245 257 177 297 229


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 6.6 7.3 6.0 6.2 8.3 8.3 8.1 8.9 8.9 8.5 8.8 9.1 9.9 12.5 15.3 13.5 15.4 17.3 7.6 7.7 9.9
Std Deviation (1e-4) 1.1 1.1 1.1 1.0 1.4 1.9 1.9 2.0 2.3 2.3 2.6 2.8 2.4 3.5 3.8 3.2 3.4 3.8 2.3 1.7 2.1
Intensity Change (Log2) – – – 0.03 0.46 0.45 0.43 0.56 0.55 0.49 0.55 0.58 0.71 1.05 1.34 1.16 1.35 1.51 0.33 0.36 0.71


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.3 -1.2 -3.4 -6.4 -8.1 -6.6 -8.2 -7.2 -8.2 2.2 1.3 2.6 0.1 0.2 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 2.1 4.1 4.8 4.7 6.1 7.3 6.7 6.5 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 4.5 6.5 4.1 4.7 5.2 4.0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 5.7479 6.9569 6.4816 6.0715 7.1281 7.1675 4.8298 5.2817 4.7073 5.269 5.2106 4.6871 5.9191 7.0232 6.3048 5.5231 6.4876 6.566
Actin 0.001 0.0394 0.0033 0.0031 0.001 0.0004 0.0175 0.0004 0.0038 0.0009 0.0002 0.0341 0.0774 0.0156 0.0284 0.0083 0.0008 0.0132
Bud 0.0001 0.0006 0.0013 0.001 0.0003 0.0001 0.0003 0.0007 0.0003 0.0001 0.0001 0.0001 0.0007 0.0001 0.0013 0.0002 0.0001 0.0001
Bud Neck 0.0002 0.0005 0.0004 0.0019 0.0012 0.001 0.0024 0.0001 0.0004 0.0003 0.0004 0.0009 0.0022 0.0001 0.0007 0.0003 0.0006 0.0008
Bud Periphery 0 0.0004 0.0005 0.0008 0.0001 0 0.0003 0.0001 0.0005 0 0 0.0001 0.0008 0 0.0007 0.0001 0 0.0001
Bud Site 0.0005 0.0059 0.0016 0.0353 0.0003 0.0001 0.0007 0.0017 0.0017 0.0001 0 0.0002 0.0022 0.0005 0.0126 0.0004 0 0.0002
Cell Periphery 0.0002 0.0001 0.0002 0.0004 0 0 0.0008 0.0001 0.0005 0 0 0 0.0007 0 0.0003 0.0001 0 0
Cytoplasm 0.8669 0.9268 0.9135 0.8936 0.8853 0.9838 0.7886 0.9049 0.9133 0.9477 0.9922 0.8453 0.7487 0.9699 0.9165 0.8749 0.9808 0.9431
Cytoplasmic Foci 0.0487 0.0046 0.0029 0.0128 0.0024 0.0016 0.0041 0.0022 0.0098 0.0038 0.0014 0.0097 0.038 0.0014 0.014 0.0219 0.0021 0.0061
Eisosomes 0 0.0001 0.0002 0.0001 0 0 0.0019 0 0.0001 0 0 0 0.0007 0 0.0001 0 0 0.0001
Endoplasmic Reticulum 0.0063 0.0014 0.0037 0.003 0.0013 0.0014 0.0189 0.0007 0.0064 0.0023 0.0009 0.0013 0.0072 0.0007 0.002 0.0035 0.0031 0.0013
Endosome 0.0028 0.0011 0.0052 0.0065 0.0007 0.0006 0.0183 0.0017 0.0198 0.0027 0.0004 0.0047 0.0292 0.0005 0.0016 0.0098 0.0011 0.0038
Golgi 0.001 0.0028 0.0014 0.0034 0.0001 0 0.0056 0.0003 0.0042 0.0001 0 0.0084 0.0191 0.002 0.0018 0.0389 0.0001 0.0062
Lipid Particles 0.0005 0.0012 0.0136 0.0068 0 0 0.0032 0 0.0055 0.0001 0 0.0047 0.0173 0 0.0012 0.0023 0 0.0041
Mitochondria 0.0003 0.0017 0.0022 0.0043 0.0002 0.0001 0.0014 0.0002 0.0029 0.0001 0 0.0089 0.0112 0.0001 0.0008 0.0026 0.0001 0.0029
None 0.0422 0.004 0.0295 0.0073 0.0957 0.0035 0.0331 0.0843 0.0171 0.0355 0.0014 0.0757 0.0126 0.0062 0.0057 0.0069 0.0032 0.0042
Nuclear Periphery 0.0013 0.0007 0.0054 0.0013 0.001 0.0008 0.0392 0.0003 0.0017 0.0012 0.0004 0.0003 0.0021 0.0003 0.0006 0.0009 0.0014 0.0009
Nucleolus 0.0001 0 0.0012 0.0012 0 0 0.0005 0 0.0002 0 0 0 0.0002 0 0.0001 0 0 0
Nucleus 0.0042 0.0023 0.0065 0.0046 0.0094 0.0059 0.0417 0.001 0.0024 0.0027 0.0021 0.001 0.0016 0.002 0.0019 0.0045 0.0058 0.0023
Peroxisomes 0.0216 0.0052 0.004 0.0085 0.0001 0 0.0003 0 0.0006 0.0001 0 0.0034 0.0216 0.0001 0.0087 0.0228 0 0.0096
Punctate Nuclear 0.0015 0.0003 0.0013 0.001 0.0004 0.0002 0.002 0.0003 0.0005 0.0012 0.0001 0.0002 0.0048 0.0002 0.0006 0.0007 0.0003 0.0005
Vacuole 0.0006 0.0006 0.0015 0.0026 0.0002 0.0003 0.0136 0.0006 0.0075 0.0011 0.0002 0.0005 0.0012 0.0003 0.0005 0.0006 0.0004 0.0002
Vacuole Periphery 0.0001 0.0001 0.0006 0.0004 0.0001 0.0001 0.0056 0.0002 0.001 0.0001 0 0.0002 0.0006 0 0.0001 0.0001 0.0001 0.0001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 17.4148 9.9634 9.7037 20.8088 7.2253 30.6332 27.0738 32.5089 33.717 21.1096
Translational Efficiency 1.1084 1.4465 1.4658 1.1525 2.2796 1.3001 1.1644 1.4655 1.4846 1.3398

Hit Data

Dataset Hit
Protein Concentration ✔
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1304 1437 512 131 1915 1219 277 919 3219 2656 789 1050

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 834.46 867.85 1891.31 1557.22 883.13 904.72 1222.62 1263.56 863.41 884.77 1656.55 1300.20
Standard Deviation 117.33 154.15 318.94 359.18 128.32 142.92 199.60 275.80 126.27 150.23 426.46 303.46
Intensity Change Log 2 — 0.056603 1.180471 0.900058 — 0.034846 0.469278 0.516796 — 0.045457 0.858351 0.715708

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000135 0.000463 0.000798 0.000583 0.000098 0.000479 0.000726 0.000378 0.000113 0.000470 0.000773 0.000404
Bud Neck 0.001948 0.005777 0.003986 0.003523 0.002038 0.005692 0.001133 0.004326 0.002002 0.005738 0.002984 0.004226
Bud Site 0.003866 0.018082 0.005393 0.009965 0.003226 0.014431 0.012615 0.034180 0.003485 0.016406 0.007928 0.031159
Cell Periphery 0.000137 0.000071 0.000507 0.000332 0.000057 0.000061 0.000106 0.000083 0.000090 0.000067 0.000366 0.000114
Cytoplasm 0.744410* 0.714831* 0.610885* 0.686205* 0.761495* 0.689298* 0.646843* 0.671950* 0.754574* 0.703113* 0.623509* 0.673728*
Cytoplasmic Foci 0.098668 0.098396 0.017847 0.023016 0.079947 0.108311 0.025317 0.023443 0.087531 0.102946 0.020469 0.023389
Eisosomes 0.000046 0.000025 0.000007 0.000016 0.000023 0.000030 0.000026 0.000013 0.000032 0.000028 0.000013 0.000013
Endoplasmic Reticulum 0.000807 0.000578 0.000755 0.000647 0.000700 0.000517 0.001093 0.000549 0.000743 0.000550 0.000874 0.000561
Endosome 0.004799 0.009081 0.001666 0.001311 0.003770 0.009194 0.003677 0.009797 0.004187 0.009133 0.002372 0.008738
Golgi 0.001762 0.001627 0.000951 0.000313 0.000837 0.004121 0.003178 0.003208 0.001211 0.002772 0.001733 0.002847
Lipid Particles 0.001539 0.001257 0.002858 0.002594 0.000938 0.000824 0.001019 0.000716 0.001181 0.001058 0.002212 0.000950
Mitochondria 0.001935 0.000990 0.000307 0.000191 0.000491 0.001922 0.000116 0.000817 0.001076 0.001418 0.000240 0.000739
Mitotic Spindle 0.001877 0.004600 0.013729 0.008699 0.001296 0.013588 0.002617 0.048753 0.001531 0.008725 0.009828 0.043756
None 0.006165 0.003163 0.007162 0.006586 0.003972 0.002033 0.004479 0.007334 0.004860 0.002644 0.006220 0.007241
Nuclear Periphery 0.000145 0.000156 0.000921 0.000342 0.000169 0.000185 0.000724 0.000688 0.000160 0.000169 0.000852 0.000645
Nuclear Periphery Foci 0.001549 0.001336 0.006234 0.005669 0.001173 0.001074 0.005382 0.002388 0.001325 0.001216 0.005935 0.002797
Nucleolus 0.000428 0.001208 0.000204 0.000150 0.000282 0.000640 0.000122 0.000230 0.000341 0.000947 0.000175 0.000220
Nucleus 0.088707 0.050937 0.284611* 0.121325 0.087455 0.041489 0.109216 0.049366 0.087962 0.046601 0.223034* 0.058344
Peroxisomes 0.000766 0.003614 0.000125 0.000137 0.001215 0.003381 0.000284 0.000802 0.001033 0.003507 0.000181 0.000719
Vacuole 0.039683 0.082455 0.040840 0.128291 0.050392 0.101601 0.181131 0.134466 0.046053 0.091242 0.090093 0.133696
Vacuole Periphery 0.000629 0.001351 0.000214 0.000104 0.000426 0.001129 0.000196 0.006512 0.000508 0.001249 0.000208 0.005713

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -3.71 -9.65 -5.59 -2.62 3.53 -2.66 -3.20 -5.30 1.38 0.96 -4.48 -10.67 -5.81 1.56 7.49
Bud Neck -6.26 -10.78 -4.41 1.26 5.12 -5.64 1.26 -2.72 3.90 -3.46 -8.50 -10.22 -3.86 5.12 6.73
Bud Site -7.73 -5.29 -6.81 2.00 -2.25 -9.27 -2.36 -5.57 -0.28 -3.17 -11.46 -6.16 -6.11 0.78 -2.88
Cell Periphery 8.34 -12.54 -3.90 -4.69 3.34 -0.54 -4.28 -5.75 -4.29 1.02 5.56 -12.75 -3.58 -5.59 11.02
Cytoplasm 5.16 21.19 9.63 7.79 0.30 9.25 16.13 17.83 8.28 -4.76 10.37 27.47 20.07 12.18 -5.73
Cytoplasmic Foci 0.03 20.10 14.17 13.53 -1.11 -5.35 19.87 21.86 21.66 -0.02 -4.18 27.22 29.57 29.07 2.55
Eisosomes 7.32 12.52 5.94 0.16 -4.62 -3.48 -3.03 5.53 7.79 6.48 2.59 7.99 11.30 9.17 2.43
Endoplasmic Reticulum 1.55 -1.43 1.26 -0.03 3.17 2.07 -4.24 4.37 2.18 7.44 2.48 -3.71 4.55 3.32 8.65
Endosome -4.39 5.52 7.87 8.74 3.39 -6.10 3.44 2.03 6.45 0.03 -7.47 6.20 3.29 8.27 0.30
Golgi 0.41 0.98 2.48 5.75 10.06 -2.62 0.34 0.63 2.79 0.03 -2.25 0.66 1.80 3.51 1.43
Lipid Particles 1.14 -8.96 -4.14 -4.44 0.62 1.40 -3.44 -3.14 -4.35 0.85 1.04 -9.88 -3.75 -4.24 7.49
Mitochondria 1.13 1.69 2.04 4.08 8.52 -2.11 2.27 1.32 2.57 -2.04 -0.42 2.00 2.19 3.65 1.37
Mitotic Spindle -2.11 -6.22 -2.59 -1.20 3.16 -4.27 -2.72 -7.15 -3.00 -5.13 -4.87 -6.98 -7.27 -4.60 -3.16
None 4.35 1.17 2.36 -1.08 1.82 2.98 -0.11 -1.95 -6.10 -1.81 4.73 -0.03 -0.60 -5.60 -0.67
Nuclear Periphery -1.95 -17.24 -9.33 -8.80 9.71 -0.85 -14.95 -11.52 -9.95 5.45 -1.38 -21.41 -13.14 -12.26 10.09
Nuclear Periphery Foci 0.80 -11.35 -5.72 -5.98 2.89 0.56 -7.87 -4.46 -4.76 5.99 0.66 -13.46 -6.32 -6.97 10.51
Nucleolus -1.76 2.31 4.89 2.47 9.30 -2.26 4.00 3.47 2.90 -1.23 -2.40 3.27 5.27 3.06 4.40
Nucleus 9.85 -23.64 -0.64 -4.00 15.12 14.45 -4.04 1.67 -10.38 4.73 16.91 -21.11 1.42 -10.48 20.71
Peroxisomes -5.22 3.18 3.60 6.82 0.78 -3.61 2.86 2.38 7.00 -2.22 -5.79 3.61 3.09 9.33 -1.51
Vacuole -13.42 -9.97 -11.61 -9.24 -8.34 -13.58 -17.40 -24.15 -15.31 4.04 -18.40 -17.26 -27.33 -19.27 -6.86
Vacuole Periphery -1.69 1.90 2.88 3.76 10.02 -2.57 5.16 -1.51 -0.27 -2.15 -2.96 3.41 -1.20 0.26 -1.91
Standard name
Human Ortholog
Description Protein involved in cell separation during budding; one of two S. cerevisiae homologs (Sds23p and Sds24p) of the S. pombe Sds23 protein, which is implicated in APC/cyclosome regulation; may play an indirect role in fluid-phase endocytosis; protein abundance increases in response to DNA replication stress; SDS24 has a paralog, SDS23, that arose from the whole genome duplication
Localization
Cell Percentages cytoplasm (96%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

Sds24

Sds24


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Sds24-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available