Standard name | |
---|---|
Human Ortholog | |
Description | GTPase-activating protein (RhoGAP) for Rho3p and Rho4p; possibly involved in control of actin cytoskeleton organization |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 21 | 34 | 3 | 6 | 12 | 7 | 12 | 23 | 8 | 9 | 7 | 15 | 24 | 31 | 23 | 10 | 14 | 6 | 28 | 35 | 7 | 34 | 61 | 65 |
Bud | 21 | 18 | 0 | 6 | 9 | 4 | 9 | 14 | 10 | 8 | 19 | 16 | 13 | 10 | 10 | 6 | 4 | 3 | 23 | 31 | 11 | 29 | 32 | 36 |
Bud Neck | 0 | 1 | 0 | 0 | 1 | 1 | 0 | 2 | 0 | 1 | 1 | 1 | 0 | 0 | 0 | 1 | 0 | 0 | 19 | 29 | 7 | 26 | 34 | 20 |
Bud Site | 1 | 4 | 0 | 0 | 0 | 1 | 1 | 2 | 2 | 2 | 1 | 1 | 1 | 1 | 0 | 1 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 4 | 9 | 1 | 1 | 15 | 9 | 17 | 11 | 9 | 14 | 12 | 21 | 14 | 23 | 28 | 10 | 15 | 10 | 3 | 1 | 1 | 1 | 2 | 2 |
Cytoplasm | 130 | 185 | 20 | 39 | 148 | 125 | 206 | 209 | 157 | 180 | 133 | 153 | 211 | 322 | 359 | 100 | 93 | 67 | 118 | 151 | 31 | 64 | 113 | 162 |
Endoplasmic Reticulum | 10 | 24 | 0 | 0 | 3 | 6 | 9 | 10 | 12 | 8 | 5 | 9 | 5 | 8 | 4 | 11 | 6 | 7 | 7 | 9 | 2 | 2 | 7 | 11 |
Endosome | 28 | 6 | 0 | 1 | 6 | 4 | 6 | 3 | 4 | 2 | 4 | 5 | 9 | 3 | 9 | 13 | 5 | 11 | 9 | 8 | 3 | 10 | 10 | 13 |
Golgi | 0 | 1 | 0 | 0 | 1 | 0 | 2 | 0 | 0 | 1 | 0 | 0 | 0 | 2 | 2 | 6 | 5 | 5 | 0 | 4 | 0 | 0 | 0 | 0 |
Mitochondria | 17 | 5 | 0 | 2 | 4 | 18 | 35 | 40 | 49 | 53 | 128 | 99 | 2 | 0 | 0 | 7 | 3 | 2 | 8 | 3 | 7 | 8 | 5 | 6 |
Nucleus | 0 | 0 | 0 | 0 | 3 | 1 | 0 | 1 | 2 | 3 | 1 | 2 | 0 | 2 | 0 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 |
Nucleolus | 3 | 0 | 0 | 0 | 1 | 2 | 0 | 2 | 1 | 1 | 1 | 0 | 0 | 1 | 0 | 3 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 7 | 6 | 0 | 0 | 5 | 2 | 3 | 2 | 5 | 3 | 2 | 4 | 3 | 4 | 4 | 9 | 8 | 0 | 0 | 0 | 0 | 0 | 0 | 1 |
SpindlePole | 2 | 0 | 0 | 1 | 9 | 7 | 4 | 15 | 6 | 8 | 2 | 3 | 9 | 10 | 13 | 15 | 11 | 8 | 2 | 1 | 0 | 10 | 2 | 3 |
Vac/Vac Membrane | 0 | 4 | 0 | 2 | 8 | 4 | 9 | 15 | 6 | 20 | 11 | 13 | 2 | 1 | 2 | 22 | 13 | 7 | 4 | 13 | 3 | 3 | 6 | 6 |
Unique Cell Count | 200 | 255 | 24 | 50 | 195 | 161 | 268 | 297 | 229 | 264 | 253 | 278 | 248 | 368 | 404 | 174 | 145 | 104 | 229 | 296 | 80 | 198 | 282 | 340 |
Labelled Cell Count | 244 | 297 | 24 | 58 | 225 | 191 | 313 | 350 | 271 | 313 | 327 | 342 | 293 | 418 | 454 | 215 | 178 | 127 | 229 | 296 | 80 | 198 | 282 | 340 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 6.6 | 8.2 | 8.8 | 7.4 | 8.6 | 7.2 | 6.8 | 6.9 | 6.2 | 6.0 | 5.4 | 5.7 | 8.6 | 8.6 | 8.4 | 9.9 | 11.0 | 9.8 | 8.1 | 8.3 | 8.4 |
Std Deviation (1e-4) | 0.9 | 1.2 | 1.6 | 1.4 | 1.9 | 1.7 | 1.8 | 1.5 | 2.0 | 1.6 | 1.5 | 1.5 | 1.3 | 1.3 | 1.4 | 2.2 | 3.4 | 2.1 | 1.1 | 1.3 | 1.5 |
Intensity Change (Log2) | – | – | – | -0.26 | -0.04 | -0.29 | -0.37 | -0.35 | -0.52 | -0.55 | -0.7 | -0.63 | -0.05 | -0.04 | -0.07 | 0.16 | 0.31 | 0.15 | -0.12 | -0.08 | -0.07 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | -0.5 | -0.8 | -0.6 | -0.7 | -1.4 | -1.5 | -1.5 | -2.9 | -2.7 | 0.2 | 0.6 | 0.8 | -2.4 | -1.9 | -1.8 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 4.8 | 3.6 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 2.0926 | 2.3539 | 1.9086 | 1.8532 | 1.9278 | 2.3542 | 2.7467 | 3.0405 | 2.8226 | 2.765 | 2.441 | 2.8246 | 2.8332 | 2.6499 | 2.4664 | 2.6809 | 2.1789 | 2.5514 |
Actin | 0.0963 | 0.0423 | 0.0949 | 0.0966 | 0.1226 | 0.0506 | 0.0884 | 0.0156 | 0.078 | 0.0458 | 0.0751 | 0.037 | 0.0723 | 0.0334 | 0.1061 | 0.0672 | 0.0656 | 0.0633 |
Bud | 0.0079 | 0.0167 | 0.1546 | 0.0239 | 0.0098 | 0.002 | 0.0396 | 0.0272 | 0.1964 | 0.0449 | 0.0393 | 0.0094 | 0.0191 | 0.015 | 0.1497 | 0.03 | 0.0052 | 0.0055 |
Bud Neck | 0.0257 | 0.0052 | 0.0042 | 0.0043 | 0.0012 | 0.408 | 0.0196 | 0.0035 | 0.0013 | 0.0038 | 0.0332 | 0.3979 | 0.0355 | 0.0082 | 0.0075 | 0.0286 | 0.1397 | 0.4065 |
Bud Periphery | 0.0107 | 0.0181 | 0.2453 | 0.0454 | 0.0062 | 0.0033 | 0.0324 | 0.0158 | 0.2797 | 0.1378 | 0.0444 | 0.0098 | 0.0399 | 0.014 | 0.209 | 0.0679 | 0.0059 | 0.0071 |
Bud Site | 0.0713 | 0.2229 | 0.0479 | 0.0042 | 0.0044 | 0.0033 | 0.0836 | 0.3258 | 0.0575 | 0.0158 | 0.0253 | 0.0073 | 0.0957 | 0.2545 | 0.0454 | 0.0082 | 0.0363 | 0.0091 |
Cell Periphery | 0.0058 | 0.0066 | 0.0072 | 0.0027 | 0.0012 | 0.002 | 0.0068 | 0.0043 | 0.0031 | 0.0049 | 0.0019 | 0.0016 | 0.0052 | 0.0026 | 0.0041 | 0.004 | 0.0012 | 0.0012 |
Cytoplasm | 0.4562 | 0.5537 | 0.244 | 0.6547 | 0.2976 | 0.3938 | 0.4307 | 0.5276 | 0.2995 | 0.5437 | 0.4805 | 0.4329 | 0.4439 | 0.5413 | 0.3441 | 0.607 | 0.5747 | 0.3427 |
Cytoplasmic Foci | 0.0891 | 0.0459 | 0.0281 | 0.021 | 0.1326 | 0.0411 | 0.0799 | 0.0303 | 0.0201 | 0.0546 | 0.1093 | 0.0588 | 0.1001 | 0.0399 | 0.0244 | 0.034 | 0.0686 | 0.0677 |
Eisosomes | 0.0005 | 0.0004 | 0.0004 | 0.0003 | 0.0022 | 0.0001 | 0.0007 | 0.0001 | 0.0002 | 0.0002 | 0.0002 | 0.0001 | 0.0005 | 0.0001 | 0.0002 | 0.0002 | 0.0005 | 0.0001 |
Endoplasmic Reticulum | 0.0147 | 0.0076 | 0.0069 | 0.0049 | 0.0094 | 0.0056 | 0.0129 | 0.0028 | 0.0028 | 0.0056 | 0.0026 | 0.0022 | 0.0113 | 0.004 | 0.0052 | 0.0051 | 0.0023 | 0.0031 |
Endosome | 0.0625 | 0.0162 | 0.0454 | 0.037 | 0.1331 | 0.0309 | 0.0632 | 0.0127 | 0.0084 | 0.0347 | 0.0322 | 0.0089 | 0.0363 | 0.0136 | 0.0157 | 0.0234 | 0.013 | 0.0144 |
Golgi | 0.0473 | 0.0082 | 0.018 | 0.0132 | 0.0399 | 0.0169 | 0.0264 | 0.0059 | 0.007 | 0.0127 | 0.0254 | 0.0049 | 0.021 | 0.007 | 0.008 | 0.0145 | 0.0047 | 0.005 |
Lipid Particles | 0.0241 | 0.0038 | 0.0174 | 0.006 | 0.0526 | 0.0037 | 0.0099 | 0.0004 | 0.0022 | 0.031 | 0.0302 | 0.0008 | 0.0144 | 0.0007 | 0.0024 | 0.0163 | 0.0018 | 0.0015 |
Mitochondria | 0.016 | 0.0016 | 0.0242 | 0.0233 | 0.03 | 0.0038 | 0.0295 | 0.0017 | 0.0189 | 0.0079 | 0.0064 | 0.0017 | 0.0124 | 0.0012 | 0.0025 | 0.0033 | 0.0009 | 0.0006 |
None | 0.0311 | 0.0414 | 0.0381 | 0.0485 | 0.1214 | 0.0276 | 0.0364 | 0.0173 | 0.0104 | 0.028 | 0.0083 | 0.0163 | 0.0473 | 0.0511 | 0.0538 | 0.0554 | 0.0483 | 0.046 |
Nuclear Periphery | 0.0075 | 0.0006 | 0.0025 | 0.0005 | 0.0019 | 0.0008 | 0.0039 | 0.0004 | 0.0006 | 0.0013 | 0.0069 | 0.0003 | 0.0085 | 0.0006 | 0.0007 | 0.0015 | 0.0008 | 0.0115 |
Nucleolus | 0.0006 | 0.0003 | 0.0013 | 0.0001 | 0.0007 | 0.0002 | 0.0011 | 0.0003 | 0.0002 | 0.0003 | 0.0148 | 0.0001 | 0.0007 | 0.0003 | 0.0003 | 0.0006 | 0.0038 | 0.0002 |
Nucleus | 0.0022 | 0.001 | 0.0017 | 0.0007 | 0.0009 | 0.0006 | 0.0043 | 0.0014 | 0.0009 | 0.0011 | 0.0182 | 0.001 | 0.0034 | 0.0014 | 0.0009 | 0.0018 | 0.0052 | 0.0004 |
Peroxisomes | 0.0177 | 0.0024 | 0.0048 | 0.0037 | 0.0206 | 0.0023 | 0.0139 | 0.0008 | 0.0063 | 0.016 | 0.0219 | 0.0051 | 0.0209 | 0.0044 | 0.0141 | 0.0135 | 0.0034 | 0.012 |
Punctate Nuclear | 0.0039 | 0.001 | 0.0012 | 0.0003 | 0.0019 | 0.0011 | 0.0038 | 0.0016 | 0.0015 | 0.0018 | 0.0192 | 0.0015 | 0.0042 | 0.0014 | 0.0018 | 0.0114 | 0.0151 | 0.0005 |
Vacuole | 0.0075 | 0.0037 | 0.0099 | 0.0074 | 0.0074 | 0.0019 | 0.01 | 0.0043 | 0.0043 | 0.0066 | 0.0034 | 0.002 | 0.0063 | 0.005 | 0.0036 | 0.005 | 0.0024 | 0.0011 |
Vacuole Periphery | 0.0015 | 0.0003 | 0.0021 | 0.0012 | 0.0024 | 0.0005 | 0.0029 | 0.0005 | 0.001 | 0.0016 | 0.0013 | 0.0005 | 0.0012 | 0.0005 | 0.0006 | 0.0008 | 0.0005 | 0.0005 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 22.3328 | 17.253 | 12.0709 | 18.4539 | 14.299 | 14.8803 | 18.9397 | 16.9879 | 19.4911 | 21.2838 |
Translational Efficiency | 0.8882 | 0.9119 | 1.4604 | 0.8003 | 1.0146 | 1.3052 | 0.9662 | 1.2266 | 1.171 | 1.0175 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
741 | 1166 | 1427 | 1374 | 2002 | 1326 | 1350 | 1087 | 2743 | 2492 | 2777 | 2461 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 781.87 | 836.15 | 1019.77 | 1090.50 | 846.77 | 870.77 | 1125.47 | 1035.67 | 829.24 | 854.57 | 1071.15 | 1066.28 |
Standard Deviation | 83.62 | 149.39 | 169.37 | 162.69 | 107.77 | 116.74 | 146.54 | 141.02 | 105.81 | 134.13 | 167.25 | 155.89 |
Intensity Change Log 2 | — | 0.096833 | 0.383243 | 0.479989 | — | 0.040322 | 0.410486 | 0.290522 | — | 0.067728 | 0.397471 | 0.384589 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.006727 | 0.002763 | 0.013337 | 0.088253 | 0.006535 | 0.005386 | 0.029857 | 0.022391 | 0.006587 | 0.004158 | 0.021368 | 0.059162 |
Bud Neck | 0.059368 | 0.028377 | 0.024922 | 0.013085 | 0.066572 | 0.068368 | 0.028190 | 0.031353 | 0.064626 | 0.049657 | 0.026511 | 0.021154 |
Bud Site | 0.241523* | 0.228554* | 0.372961* | 0.316964* | 0.233141* | 0.272767* | 0.321391* | 0.324287* | 0.235406* | 0.252080* | 0.347891* | 0.320199* |
Cell Periphery | 0.002026 | 0.000286 | 0.000424 | 0.000551 | 0.001218 | 0.000556 | 0.000433 | 0.000361 | 0.001436 | 0.000430 | 0.000428 | 0.000467 |
Cytoplasm | 0.428183* | 0.436706* | 0.385467* | 0.396154* | 0.436497* | 0.405932* | 0.245965* | 0.252214* | 0.434251* | 0.420331* | 0.317650* | 0.332577* |
Cytoplasmic Foci | 0.156941 | 0.174528 | 0.084902 | 0.077548 | 0.157931 | 0.124596 | 0.142747 | 0.099191 | 0.157663 | 0.147959 | 0.113023 | 0.087107 |
Eisosomes | 0.001775 | 0.000063 | 0.000093 | 0.000136 | 0.001281 | 0.000279 | 0.000161 | 0.000095 | 0.001415 | 0.000178 | 0.000126 | 0.000118 |
Endoplasmic Reticulum | 0.003777 | 0.001494 | 0.002329 | 0.000477 | 0.002823 | 0.000997 | 0.003359 | 0.001004 | 0.003081 | 0.001229 | 0.002830 | 0.000710 |
Endosome | 0.020238 | 0.029767 | 0.012993 | 0.011773 | 0.015753 | 0.025496 | 0.048423 | 0.045603 | 0.016965 | 0.027494 | 0.030217 | 0.026715 |
Golgi | 0.023535 | 0.010684 | 0.003472 | 0.007424 | 0.022566 | 0.015337 | 0.008702 | 0.012915 | 0.022827 | 0.013160 | 0.006015 | 0.009849 |
Lipid Particles | 0.004429 | 0.002652 | 0.002464 | 0.002323 | 0.003104 | 0.002311 | 0.007676 | 0.004406 | 0.003462 | 0.002470 | 0.004998 | 0.003243 |
Mitochondria | 0.006140 | 0.002801 | 0.000362 | 0.000680 | 0.005676 | 0.003610 | 0.001046 | 0.001501 | 0.005801 | 0.003231 | 0.000695 | 0.001043 |
Mitotic Spindle | 0.000307 | 0.004525 | 0.005450 | 0.012000 | 0.001196 | 0.004682 | 0.009080 | 0.024343 | 0.000956 | 0.004608 | 0.007215 | 0.017452 |
None | 0.000566 | 0.002959 | 0.000257 | 0.000111 | 0.000616 | 0.000382 | 0.000257 | 0.000344 | 0.000602 | 0.001588 | 0.000257 | 0.000214 |
Nuclear Periphery | 0.000067 | 0.000154 | 0.000139 | 0.000044 | 0.000019 | 0.000029 | 0.000227 | 0.000162 | 0.000032 | 0.000087 | 0.000182 | 0.000096 |
Nuclear Periphery Foci | 0.000327 | 0.001431 | 0.002434 | 0.000448 | 0.000257 | 0.000238 | 0.002693 | 0.001458 | 0.000276 | 0.000796 | 0.002560 | 0.000894 |
Nucleolus | 0.000180 | 0.001479 | 0.000161 | 0.000062 | 0.000138 | 0.000207 | 0.000240 | 0.000243 | 0.000149 | 0.000802 | 0.000199 | 0.000142 |
Nucleus | 0.005174 | 0.008831 | 0.009476 | 0.002126 | 0.006344 | 0.003371 | 0.004806 | 0.003595 | 0.006028 | 0.005926 | 0.007206 | 0.002775 |
Peroxisomes | 0.008616 | 0.013670 | 0.012486 | 0.033033 | 0.008735 | 0.012967 | 0.025396 | 0.052478 | 0.008703 | 0.013296 | 0.018762 | 0.041622 |
Vacuole | 0.026810 | 0.044704 | 0.064930 | 0.035585 | 0.027168 | 0.049588 | 0.117497 | 0.120008 | 0.027071 | 0.047303 | 0.090485 | 0.072874 |
Vacuole Periphery | 0.003291 | 0.003573 | 0.000941 | 0.001221 | 0.002430 | 0.002904 | 0.001852 | 0.002047 | 0.002663 | 0.003217 | 0.001384 | 0.001586 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | 3.37 | -3.53 | -12.84 | -13.71 | -11.62 | 1.04 | -7.87 | -5.03 | -5.27 | 1.99 | 3.17 | -8.67 | -13.68 | -14.37 | -8.98 |
Bud Neck | 5.75 | 6.99 | 9.43 | 6.58 | 6.74 | -0.31 | 10.78 | 9.58 | 8.78 | -0.63 | 4.19 | 13.40 | 15.32 | 11.46 | 3.50 |
Bud Site | 1.18 | -7.65 | -3.45 | -5.32 | 5.04 | -3.86 | -6.28 | -5.21 | -1.79 | 0.33 | -1.90 | -10.84 | -6.71 | -4.69 | 3.77 |
Cell Periphery | 7.51 | 7.04 | 5.85 | -1.61 | -0.67 | 5.87 | 7.34 | 7.18 | 2.81 | 0.97 | 9.74 | 10.14 | 8.06 | -0.07 | -0.24 |
Cytoplasm | -0.72 | 2.72 | 0.82 | 1.64 | -2.06 | 2.93 | 18.02 | 15.39 | 11.22 | -1.02 | 1.60 | 13.43 | 9.78 | 7.65 | -2.90 |
Cytoplasmic Foci | -2.07 | 10.14 | 11.22 | 14.09 | 1.68 | 5.65 | 2.57 | 9.74 | 3.93 | 6.55 | 1.98 | 10.03 | 16.18 | 13.10 | 6.43 |
Eisosomes | 6.40 | 6.30 | 6.16 | -5.80 | -3.01 | 5.43 | 6.13 | 6.49 | 8.11 | 4.03 | 8.11 | 8.49 | 8.56 | 4.94 | 1.15 |
Endoplasmic Reticulum | 2.62 | 1.37 | 3.84 | 3.70 | 3.27 | 3.09 | -0.49 | 3.37 | 0.19 | 4.29 | 3.82 | 0.52 | 5.13 | 2.70 | 5.34 |
Endosome | -3.36 | 3.61 | 4.62 | 7.92 | 1.40 | -6.07 | -10.98 | -8.60 | -4.96 | 1.10 | -7.22 | -7.50 | -4.64 | 1.54 | 2.23 |
Golgi | 7.27 | 12.24 | 9.41 | 3.82 | -3.69 | 5.12 | 12.54 | 6.06 | 2.04 | -1.91 | 9.32 | 19.42 | 12.24 | 4.18 | -3.30 |
Lipid Particles | 2.15 | 2.28 | 2.24 | -0.13 | -0.15 | 1.45 | -5.09 | -1.35 | -1.97 | 2.69 | 2.35 | -3.30 | -0.05 | -1.81 | 2.70 |
Mitochondria | 2.87 | 6.50 | 6.20 | 2.78 | -2.22 | 2.34 | 6.56 | 7.19 | 3.09 | -0.77 | 3.71 | 9.87 | 10.04 | 4.32 | -1.15 |
Mitotic Spindle | -5.30 | -6.08 | -6.17 | -3.57 | -2.66 | -3.13 | -5.70 | -7.40 | -5.97 | -4.05 | -4.89 | -7.51 | -9.32 | -6.83 | -4.69 |
None | -2.92 | 3.29 | 4.97 | 3.49 | 4.29 | 1.75 | 2.72 | 1.85 | 0.18 | -0.74 | -2.52 | 3.97 | 4.37 | 3.51 | 0.47 |
Nuclear Periphery | -1.35 | -1.34 | 0.32 | 1.72 | 1.73 | -2.10 | -3.70 | -4.08 | -3.75 | 0.98 | -1.98 | -3.87 | -3.81 | -0.46 | 2.02 |
Nuclear Periphery Foci | -3.67 | -8.35 | -3.76 | 2.63 | 7.23 | 0.16 | -6.72 | -3.15 | -3.16 | 2.41 | -3.65 | -10.55 | -4.10 | -0.95 | 5.99 |
Nucleolus | -1.96 | -0.06 | 1.21 | 2.15 | 2.87 | -1.41 | -2.00 | -2.03 | -0.44 | 0.47 | -2.13 | -1.79 | -0.01 | 2.13 | 1.80 |
Nucleus | -2.98 | -4.87 | 1.38 | 5.78 | 8.35 | 6.11 | 1.63 | 3.50 | -1.67 | 1.31 | -0.13 | -4.09 | 4.60 | 3.85 | 7.66 |
Peroxisomes | -2.96 | -2.43 | -9.35 | -6.48 | -7.76 | -2.96 | -8.09 | -9.26 | -7.98 | -5.24 | -4.17 | -7.92 | -12.93 | -10.30 | -8.32 |
Vacuole | -5.96 | -12.42 | -8.01 | -2.59 | 4.66 | -7.88 | -18.94 | -17.01 | -12.88 | -1.35 | -10.03 | -22.62 | -18.34 | -11.59 | 2.10 |
Vacuole Periphery | -0.36 | 5.71 | 4.47 | 3.89 | -0.49 | -1.21 | 2.52 | 1.73 | 2.27 | -0.34 | -1.50 | 6.57 | 4.70 | 4.61 | -0.44 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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