Standard name | |
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Human Ortholog | |
Description | GTPase-activating protein (RhoGAP) for Rho3p and Rho4p; possibly involved in control of actin cytoskeleton organization |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 21 | 34 | 3 | 6 | 12 | 7 | 12 | 23 | 8 | 9 | 7 | 15 | 24 | 31 | 23 | 10 | 14 | 6 | 28 | 35 | 7 | 34 | 61 | 65 |
Bud | 21 | 18 | 0 | 6 | 9 | 4 | 9 | 14 | 10 | 8 | 19 | 16 | 13 | 10 | 10 | 6 | 4 | 3 | 23 | 31 | 11 | 29 | 32 | 36 |
Bud Neck | 0 | 1 | 0 | 0 | 1 | 1 | 0 | 2 | 0 | 1 | 1 | 1 | 0 | 0 | 0 | 1 | 0 | 0 | 19 | 29 | 7 | 26 | 34 | 20 |
Bud Site | 1 | 4 | 0 | 0 | 0 | 1 | 1 | 2 | 2 | 2 | 1 | 1 | 1 | 1 | 0 | 1 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 4 | 9 | 1 | 1 | 15 | 9 | 17 | 11 | 9 | 14 | 12 | 21 | 14 | 23 | 28 | 10 | 15 | 10 | 3 | 1 | 1 | 1 | 2 | 2 |
Cytoplasm | 130 | 185 | 20 | 39 | 148 | 125 | 206 | 209 | 157 | 180 | 133 | 153 | 211 | 322 | 359 | 100 | 93 | 67 | 118 | 151 | 31 | 64 | 113 | 162 |
Endoplasmic Reticulum | 10 | 24 | 0 | 0 | 3 | 6 | 9 | 10 | 12 | 8 | 5 | 9 | 5 | 8 | 4 | 11 | 6 | 7 | 7 | 9 | 2 | 2 | 7 | 11 |
Endosome | 28 | 6 | 0 | 1 | 6 | 4 | 6 | 3 | 4 | 2 | 4 | 5 | 9 | 3 | 9 | 13 | 5 | 11 | 9 | 8 | 3 | 10 | 10 | 13 |
Golgi | 0 | 1 | 0 | 0 | 1 | 0 | 2 | 0 | 0 | 1 | 0 | 0 | 0 | 2 | 2 | 6 | 5 | 5 | 0 | 4 | 0 | 0 | 0 | 0 |
Mitochondria | 17 | 5 | 0 | 2 | 4 | 18 | 35 | 40 | 49 | 53 | 128 | 99 | 2 | 0 | 0 | 7 | 3 | 2 | 8 | 3 | 7 | 8 | 5 | 6 |
Nucleus | 0 | 0 | 0 | 0 | 3 | 1 | 0 | 1 | 2 | 3 | 1 | 2 | 0 | 2 | 0 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 |
Nucleolus | 3 | 0 | 0 | 0 | 1 | 2 | 0 | 2 | 1 | 1 | 1 | 0 | 0 | 1 | 0 | 3 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 7 | 6 | 0 | 0 | 5 | 2 | 3 | 2 | 5 | 3 | 2 | 4 | 3 | 4 | 4 | 9 | 8 | 0 | 0 | 0 | 0 | 0 | 0 | 1 |
SpindlePole | 2 | 0 | 0 | 1 | 9 | 7 | 4 | 15 | 6 | 8 | 2 | 3 | 9 | 10 | 13 | 15 | 11 | 8 | 2 | 1 | 0 | 10 | 2 | 3 |
Vac/Vac Membrane | 0 | 4 | 0 | 2 | 8 | 4 | 9 | 15 | 6 | 20 | 11 | 13 | 2 | 1 | 2 | 22 | 13 | 7 | 4 | 13 | 3 | 3 | 6 | 6 |
Unique Cell Count | 200 | 255 | 24 | 50 | 195 | 161 | 268 | 297 | 229 | 264 | 253 | 278 | 248 | 368 | 404 | 174 | 145 | 104 | 229 | 296 | 80 | 198 | 282 | 340 |
Labelled Cell Count | 244 | 297 | 24 | 58 | 225 | 191 | 313 | 350 | 271 | 313 | 327 | 342 | 293 | 418 | 454 | 215 | 178 | 127 | 229 | 296 | 80 | 198 | 282 | 340 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 6.6 | 8.2 | 8.8 | 7.4 | 8.6 | 7.2 | 6.8 | 6.9 | 6.2 | 6.0 | 5.4 | 5.7 | 8.6 | 8.6 | 8.4 | 9.9 | 11.0 | 9.8 | 8.1 | 8.3 | 8.4 |
Std Deviation (1e-4) | 0.9 | 1.2 | 1.6 | 1.4 | 1.9 | 1.7 | 1.8 | 1.5 | 2.0 | 1.6 | 1.5 | 1.5 | 1.3 | 1.3 | 1.4 | 2.2 | 3.4 | 2.1 | 1.1 | 1.3 | 1.5 |
Intensity Change (Log2) | – | – | – | -0.26 | -0.04 | -0.29 | -0.37 | -0.35 | -0.52 | -0.55 | -0.7 | -0.63 | -0.05 | -0.04 | -0.07 | 0.16 | 0.31 | 0.15 | -0.12 | -0.08 | -0.07 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | -0.5 | -0.8 | -0.6 | -0.7 | -1.4 | -1.5 | -1.5 | -2.9 | -2.7 | 0.2 | 0.6 | 0.8 | -2.4 | -1.9 | -1.8 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 4.8 | 3.6 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 2.0926 | 2.3539 | 1.9086 | 1.8532 | 1.9278 | 2.3542 | 2.7467 | 3.0405 | 2.8226 | 2.765 | 2.441 | 2.8246 | 2.8332 | 2.6499 | 2.4664 | 2.6809 | 2.1789 | 2.5514 |
Actin | 0.0963 | 0.0423 | 0.0949 | 0.0966 | 0.1226 | 0.0506 | 0.0884 | 0.0156 | 0.078 | 0.0458 | 0.0751 | 0.037 | 0.0723 | 0.0334 | 0.1061 | 0.0672 | 0.0656 | 0.0633 |
Bud | 0.0079 | 0.0167 | 0.1546 | 0.0239 | 0.0098 | 0.002 | 0.0396 | 0.0272 | 0.1964 | 0.0449 | 0.0393 | 0.0094 | 0.0191 | 0.015 | 0.1497 | 0.03 | 0.0052 | 0.0055 |
Bud Neck | 0.0257 | 0.0052 | 0.0042 | 0.0043 | 0.0012 | 0.408 | 0.0196 | 0.0035 | 0.0013 | 0.0038 | 0.0332 | 0.3979 | 0.0355 | 0.0082 | 0.0075 | 0.0286 | 0.1397 | 0.4065 |
Bud Periphery | 0.0107 | 0.0181 | 0.2453 | 0.0454 | 0.0062 | 0.0033 | 0.0324 | 0.0158 | 0.2797 | 0.1378 | 0.0444 | 0.0098 | 0.0399 | 0.014 | 0.209 | 0.0679 | 0.0059 | 0.0071 |
Bud Site | 0.0713 | 0.2229 | 0.0479 | 0.0042 | 0.0044 | 0.0033 | 0.0836 | 0.3258 | 0.0575 | 0.0158 | 0.0253 | 0.0073 | 0.0957 | 0.2545 | 0.0454 | 0.0082 | 0.0363 | 0.0091 |
Cell Periphery | 0.0058 | 0.0066 | 0.0072 | 0.0027 | 0.0012 | 0.002 | 0.0068 | 0.0043 | 0.0031 | 0.0049 | 0.0019 | 0.0016 | 0.0052 | 0.0026 | 0.0041 | 0.004 | 0.0012 | 0.0012 |
Cytoplasm | 0.4562 | 0.5537 | 0.244 | 0.6547 | 0.2976 | 0.3938 | 0.4307 | 0.5276 | 0.2995 | 0.5437 | 0.4805 | 0.4329 | 0.4439 | 0.5413 | 0.3441 | 0.607 | 0.5747 | 0.3427 |
Cytoplasmic Foci | 0.0891 | 0.0459 | 0.0281 | 0.021 | 0.1326 | 0.0411 | 0.0799 | 0.0303 | 0.0201 | 0.0546 | 0.1093 | 0.0588 | 0.1001 | 0.0399 | 0.0244 | 0.034 | 0.0686 | 0.0677 |
Eisosomes | 0.0005 | 0.0004 | 0.0004 | 0.0003 | 0.0022 | 0.0001 | 0.0007 | 0.0001 | 0.0002 | 0.0002 | 0.0002 | 0.0001 | 0.0005 | 0.0001 | 0.0002 | 0.0002 | 0.0005 | 0.0001 |
Endoplasmic Reticulum | 0.0147 | 0.0076 | 0.0069 | 0.0049 | 0.0094 | 0.0056 | 0.0129 | 0.0028 | 0.0028 | 0.0056 | 0.0026 | 0.0022 | 0.0113 | 0.004 | 0.0052 | 0.0051 | 0.0023 | 0.0031 |
Endosome | 0.0625 | 0.0162 | 0.0454 | 0.037 | 0.1331 | 0.0309 | 0.0632 | 0.0127 | 0.0084 | 0.0347 | 0.0322 | 0.0089 | 0.0363 | 0.0136 | 0.0157 | 0.0234 | 0.013 | 0.0144 |
Golgi | 0.0473 | 0.0082 | 0.018 | 0.0132 | 0.0399 | 0.0169 | 0.0264 | 0.0059 | 0.007 | 0.0127 | 0.0254 | 0.0049 | 0.021 | 0.007 | 0.008 | 0.0145 | 0.0047 | 0.005 |
Lipid Particles | 0.0241 | 0.0038 | 0.0174 | 0.006 | 0.0526 | 0.0037 | 0.0099 | 0.0004 | 0.0022 | 0.031 | 0.0302 | 0.0008 | 0.0144 | 0.0007 | 0.0024 | 0.0163 | 0.0018 | 0.0015 |
Mitochondria | 0.016 | 0.0016 | 0.0242 | 0.0233 | 0.03 | 0.0038 | 0.0295 | 0.0017 | 0.0189 | 0.0079 | 0.0064 | 0.0017 | 0.0124 | 0.0012 | 0.0025 | 0.0033 | 0.0009 | 0.0006 |
None | 0.0311 | 0.0414 | 0.0381 | 0.0485 | 0.1214 | 0.0276 | 0.0364 | 0.0173 | 0.0104 | 0.028 | 0.0083 | 0.0163 | 0.0473 | 0.0511 | 0.0538 | 0.0554 | 0.0483 | 0.046 |
Nuclear Periphery | 0.0075 | 0.0006 | 0.0025 | 0.0005 | 0.0019 | 0.0008 | 0.0039 | 0.0004 | 0.0006 | 0.0013 | 0.0069 | 0.0003 | 0.0085 | 0.0006 | 0.0007 | 0.0015 | 0.0008 | 0.0115 |
Nucleolus | 0.0006 | 0.0003 | 0.0013 | 0.0001 | 0.0007 | 0.0002 | 0.0011 | 0.0003 | 0.0002 | 0.0003 | 0.0148 | 0.0001 | 0.0007 | 0.0003 | 0.0003 | 0.0006 | 0.0038 | 0.0002 |
Nucleus | 0.0022 | 0.001 | 0.0017 | 0.0007 | 0.0009 | 0.0006 | 0.0043 | 0.0014 | 0.0009 | 0.0011 | 0.0182 | 0.001 | 0.0034 | 0.0014 | 0.0009 | 0.0018 | 0.0052 | 0.0004 |
Peroxisomes | 0.0177 | 0.0024 | 0.0048 | 0.0037 | 0.0206 | 0.0023 | 0.0139 | 0.0008 | 0.0063 | 0.016 | 0.0219 | 0.0051 | 0.0209 | 0.0044 | 0.0141 | 0.0135 | 0.0034 | 0.012 |
Punctate Nuclear | 0.0039 | 0.001 | 0.0012 | 0.0003 | 0.0019 | 0.0011 | 0.0038 | 0.0016 | 0.0015 | 0.0018 | 0.0192 | 0.0015 | 0.0042 | 0.0014 | 0.0018 | 0.0114 | 0.0151 | 0.0005 |
Vacuole | 0.0075 | 0.0037 | 0.0099 | 0.0074 | 0.0074 | 0.0019 | 0.01 | 0.0043 | 0.0043 | 0.0066 | 0.0034 | 0.002 | 0.0063 | 0.005 | 0.0036 | 0.005 | 0.0024 | 0.0011 |
Vacuole Periphery | 0.0015 | 0.0003 | 0.0021 | 0.0012 | 0.0024 | 0.0005 | 0.0029 | 0.0005 | 0.001 | 0.0016 | 0.0013 | 0.0005 | 0.0012 | 0.0005 | 0.0006 | 0.0008 | 0.0005 | 0.0005 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 22.3328 | 17.253 | 12.0709 | 18.4539 | 14.299 | 14.8803 | 18.9397 | 16.9879 | 19.4911 | 21.2838 |
Translational Efficiency | 0.8882 | 0.9119 | 1.4604 | 0.8003 | 1.0146 | 1.3052 | 0.9662 | 1.2266 | 1.171 | 1.0175 |
Dataset | Hit |
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Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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