Standard name | |
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Human Ortholog | |
Description | Third-largest subunit of DNA polymerase II (DNA polymerase epsilon); required to maintain fidelity of chromosomal replication and also for inheritance of telomeric silencing; stabilizes the interaction of Pol epsilon with primer-template DNA, positively affecting the processivity of the polymerase and exonuclease activities of Pol epsilon; mRNA abundance peaks at the G1/S boundary of the cell cycle; DPB3 has a paralog, DLS1, that arose from the whole genome duplication |
ensLOC | DeepLoc | |||||||||||||||||||||||
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Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 2 | 3 | – | 6 | 3 | 9 | 3 | 0 | 0 | 0 | 0 | 1 | 0 | 2 | 0 | 1 | 1 | 5 | 6 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 1 | 1 | 3 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 3 |
Bud Site | 0 | 0 | 0 | 2 | 1 | 11 | 1 | – | 7 | 13 | 16 | 26 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 3 | 5 | 13 | 16 | 22 | 33 | 29 | – | 26 | 27 | 30 | 13 | 25 | 68 | 58 | 14 | 21 | 13 | 1 | 0 | 3 | 3 | 4 | 3 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 5 | 7 | 4 | 0 | 0 | 0 | 0 | 0 | 1 |
Endosome | 1 | 0 | 1 | 4 | 9 | 2 | 3 | – | 1 | 1 | 3 | 3 | 0 | 3 | 3 | 4 | 3 | 0 | 5 | 2 | 0 | 1 | 6 | 9 |
Golgi | 1 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 1 | 1 |
Mitochondria | 7 | 9 | 4 | 7 | 10 | 135 | 59 | – | 152 | 142 | 197 | 203 | 5 | 15 | 2 | 9 | 4 | 9 | 1 | 1 | 1 | 0 | 9 | 11 |
Nucleus | 269 | 142 | 305 | 293 | 304 | 307 | 437 | – | 408 | 423 | 468 | 441 | 220 | 301 | 292 | 222 | 206 | 173 | 256 | 144 | 298 | 141 | 229 | 165 |
Nuclear Periphery | 1 | 0 | 0 | 0 | 2 | 2 | 7 | – | 5 | 5 | 5 | 4 | 0 | 1 | 1 | 1 | 0 | 3 | 0 | 0 | 0 | 0 | 0 | 2 |
Nucleolus | 3 | 1 | 1 | 0 | 2 | 5 | 8 | – | 15 | 11 | 12 | 13 | 2 | 4 | 10 | 4 | 3 | 1 | 7 | 0 | 6 | 1 | 12 | 13 |
Peroxisomes | 1 | 0 | 0 | 1 | 0 | 0 | 1 | – | 0 | 0 | 0 | 0 | 0 | 0 | 4 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 4 | 6 | 5 | 4 | 12 | – | 3 | 9 | 3 | 7 | 0 | 4 | 4 | 3 | 1 | 0 | 2 | 0 | 3 | 1 | 5 | 7 |
Vac/Vac Membrane | 6 | 10 | 8 | 7 | 13 | 9 | 11 | – | 20 | 9 | 17 | 21 | 7 | 8 | 9 | 15 | 13 | 10 | 16 | 8 | 30 | 28 | 73 | 69 |
Unique Cell Count | 284 | 156 | 327 | 322 | 352 | 389 | 498 | 517 | 519 | 599 | 559 | 249 | 382 | 368 | 257 | 243 | 195 | 298 | 162 | 350 | 184 | 356 | 303 | |
Labelled Cell Count | 292 | 167 | 336 | 336 | 368 | 510 | 571 | 644 | 644 | 763 | 735 | 259 | 404 | 383 | 277 | 259 | 214 | 298 | 162 | 350 | 184 | 356 | 303 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 5.4 | 5.2 | 6.0 | 5.7 | 6.4 | 4.3 | 5.2 | – | 4.7 | 4.9 | 4.7 | 4.6 | 6.4 | 5.5 | 6.7 | 7.0 | 7.9 | 6.8 | 5.3 | 5.7 | 5.9 |
Std Deviation (1e-4) | 0.8 | 0.7 | 1.2 | 1.4 | 3.7 | 1.2 | 1.4 | – | 1.2 | 1.1 | 1.0 | 0.9 | 1.6 | 1.6 | 2.0 | 1.4 | 1.9 | 1.4 | 1.5 | 1.7 | 1.6 |
Intensity Change (Log2) | – | – | – | -0.07 | 0.09 | -0.48 | -0.2 | – | -0.36 | -0.3 | -0.36 | -0.39 | 0.09 | -0.12 | 0.16 | 0.23 | 0.41 | 0.17 | -0.17 | -0.07 | -0.03 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 0.6 | 1.3 | 2.5 | 1.2 | – | 0.7 | 0.8 | 0.7 | -1.4 | 2.9 | 5.8 | 5.1 | 0.8 | 2.3 | 1.4 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0.9 | 1.5 | 0 | 5.6 | – | 0 | 0 | 0 | 0 | 0 | 2.2 | 0 | 1.9 | 0 | 0 |
Nucleus | -1.1 | -3.0 | -5.4 | -2.6 | – | -5.6 | -4.8 | -5.9 | -5.7 | -2.1 | -5.5 | -5.3 | -2.8 | -3.3 | -1.8 |
Nuclear Periphery | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 2.5 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 1.2 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | -0.2 | 0.9 | -0.1 | -0.2 | – | 1.1 | -0.7 | 0.4 | 1.1 | 0.3 | -0.3 | 0 | 2.1 | 1.8 | 1.6 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 0.6374 | 0.3493 | 0.3408 | 0.3504 | 0.7979 | 0.2912 | 3.5936 | 3.8457 | 3.74 | 3.981 | 3.6865 | 3.6867 | 3.5714 | 4.8955 | 4.4745 | 4.1044 | 4.4675 | 4.5753 |
Actin | 0.051 | 0.0001 | 0.0033 | 0.0005 | 0.0028 | 0.0087 | 0.0192 | 0.0002 | 0.0083 | 0.0053 | 0.0157 | 0.0093 | 0.0224 | 0 | 0.0001 | 0 | 0 | 0.0058 |
Bud | 0.0011 | 0 | 0.0001 | 0.0002 | 0.0001 | 0.0007 | 0.0004 | 0 | 0 | 0.0001 | 0.0002 | 0.0001 | 0.0001 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0.0034 | 0.0002 | 0.0004 | 0.0007 | 0.0009 | 0.0022 | 0.0045 | 0.0006 | 0.0003 | 0.0055 | 0.0007 | 0.0079 | 0.0009 | 0.0003 | 0.0001 | 0 | 0.0004 | 0.0011 |
Bud Periphery | 0.0012 | 0 | 0.0001 | 0.0001 | 0.0001 | 0.002 | 0.0007 | 0 | 0.0001 | 0.0002 | 0.0004 | 0.0001 | 0.0002 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0.013 | 0.0001 | 0.0006 | 0.0039 | 0.0005 | 0.0014 | 0.0018 | 0.0002 | 0.0002 | 0.0002 | 0.0006 | 0.0003 | 0.0005 | 0.0003 | 0.0002 | 0 | 0 | 0.0001 |
Cell Periphery | 0.0004 | 0 | 0 | 0 | 0 | 0.0002 | 0.0002 | 0 | 0 | 0 | 0.0001 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 0.0163 | 0.0118 | 0.014 | 0.0009 | 0.0011 | 0.0042 | 0.014 | 0.0143 | 0.0068 | 0.0063 | 0.0115 | 0.0029 | 0.0045 | 0.0134 | 0.0087 | 0.0001 | 0.0005 | 0.0042 |
Cytoplasmic Foci | 0.0139 | 0.0001 | 0.0012 | 0.028 | 0.0198 | 0.0032 | 0.0032 | 0.0002 | 0.0021 | 0.0008 | 0.0042 | 0.0016 | 0.0014 | 0.0001 | 0.0002 | 0 | 0 | 0.0006 |
Eisosomes | 0.0003 | 0 | 0 | 0 | 0 | 0.0001 | 0.0003 | 0 | 0.0001 | 0.0001 | 0.0002 | 0 | 0.0002 | 0 | 0 | 0 | 0 | 0.0001 |
Endoplasmic Reticulum | 0.0016 | 0.0004 | 0.0035 | 0 | 0.0013 | 0.0008 | 0.0042 | 0.0005 | 0.0008 | 0.0006 | 0.0031 | 0.0002 | 0.0007 | 0.0001 | 0.0013 | 0 | 0 | 0.0003 |
Endosome | 0.0177 | 0.0003 | 0.0054 | 0.003 | 0.0319 | 0.0051 | 0.009 | 0.0005 | 0.0021 | 0.0023 | 0.0112 | 0.0015 | 0.0024 | 0.0001 | 0.0003 | 0 | 0 | 0.001 |
Golgi | 0.0078 | 0 | 0.0005 | 0.0027 | 0.014 | 0.0031 | 0.0015 | 0 | 0.0017 | 0.0012 | 0.0031 | 0.0014 | 0.001 | 0 | 0 | 0 | 0 | 0.0005 |
Lipid Particles | 0.0124 | 0 | 0.0013 | 0.0036 | 0.0809 | 0.0031 | 0.0035 | 0 | 0.0025 | 0.0027 | 0.006 | 0.0032 | 0.0019 | 0 | 0.0001 | 0 | 0 | 0.0017 |
Mitochondria | 0.0031 | 0.0001 | 0.0012 | 0.0005 | 0.0028 | 0.0066 | 0.0014 | 0.0003 | 0.0006 | 0.0006 | 0.0043 | 0.0005 | 0.0017 | 0.0001 | 0.0002 | 0 | 0.0001 | 0.0002 |
None | 0.0084 | 0.0014 | 0.0029 | 0.0003 | 0.0004 | 0.0193 | 0.0302 | 0.0179 | 0.0039 | 0.0004 | 0.007 | 0.0051 | 0.0012 | 0.0095 | 0.0237 | 0 | 0.0001 | 0.0079 |
Nuclear Periphery | 0.0281 | 0.0096 | 0.0187 | 0.0054 | 0.0784 | 0.0047 | 0.0252 | 0.0076 | 0.0033 | 0.0149 | 0.0265 | 0.0037 | 0.0051 | 0.0044 | 0.0076 | 0.0012 | 0.002 | 0.0028 |
Nucleolus | 0.0059 | 0.0057 | 0.0262 | 0.0048 | 0.0037 | 0.0191 | 0.0078 | 0.0065 | 0.0121 | 0.0078 | 0.01 | 0.0184 | 0.0063 | 0.0072 | 0.0109 | 0.0021 | 0.0194 | 0.016 |
Nucleus | 0.7919 | 0.9588 | 0.8963 | 0.932 | 0.7425 | 0.8968 | 0.851 | 0.9397 | 0.9336 | 0.9365 | 0.8669 | 0.9156 | 0.9329 | 0.9558 | 0.9407 | 0.9964 | 0.965 | 0.9388 |
Peroxisomes | 0.0084 | 0 | 0.0001 | 0.0081 | 0.0099 | 0.009 | 0.0029 | 0 | 0.0014 | 0.0008 | 0.0015 | 0.0044 | 0.0018 | 0 | 0 | 0 | 0 | 0.0009 |
Punctate Nuclear | 0.0103 | 0.011 | 0.0229 | 0.005 | 0.0064 | 0.0064 | 0.0158 | 0.0106 | 0.0198 | 0.013 | 0.025 | 0.0235 | 0.0143 | 0.0082 | 0.0055 | 0.0001 | 0.0125 | 0.0177 |
Vacuole | 0.0026 | 0.0003 | 0.0007 | 0.0002 | 0.0012 | 0.0023 | 0.0025 | 0.0004 | 0.0002 | 0.0003 | 0.001 | 0.0002 | 0.0004 | 0.0004 | 0.0002 | 0 | 0 | 0.0001 |
Vacuole Periphery | 0.0013 | 0.0001 | 0.0004 | 0.0001 | 0.0013 | 0.0008 | 0.001 | 0.0001 | 0.0001 | 0.0003 | 0.001 | 0.0001 | 0.0002 | 0.0001 | 0.0001 | 0 | 0 | 0.0001 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 30.0432 | 17.9045 | 15.7015 | 16.1947 | 20.0663 | 30.9365 | 11.1088 | 13.8846 | 17.3944 | 22.071 |
Translational Efficiency | 1.1426 | 0.9522 | 0.9185 | 1.1708 | 1.298 | 1.0715 | 1.4092 | 1.2631 | 1.0385 | 1.2037 |
Dataset | Hit |
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Protein Concentration | ✔ |
Protein Localization | ✘ |
Gene Expression | ✔ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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