Standard name
Human Ortholog
Description Mu3-like subunit of the clathrin associated protein complex (AP-3); functions in transport of alkaline phosphatase to the vacuole via the alternate pathway
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.08 0.08 0.05 0 0 0.05 0.05 0 0
Bud 0 0 0.06 0.05 0.06 0.06 0.06 0.08 0.11 0.09 0.11 0.09 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0.08 0.08 0 0.08 0 0 0 0 0 0.05 0 0 0.06 0.08 0.07 0.06 0.1 0 0 0 0 0 0
Endoplasmic Reticulum 0 0.06 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0.06 0 0.19 0.05 0 0.06 0 0 0 0 0.06 0.14 0.31 0.3 0.15 0.21 0.23 0.19 0.19 0.22 0.16 0.15
Golgi 0 0.05 0 0.07 0 0 0 0 0 0 0 0 0.25 0.33 0.27 0.41 0.55 0.43 0.5 0.28 0.49 0.38 0.52 0.56
Mitochondria 0.96 0.88 0.84 0.9 0.75 0.9 0.89 0.84 0.91 0.89 0.88 0.87 0.73 0.47 0.27 0.16 0.1 0.09 0.17 0.33 0.13 0.18 0.16 0.14
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0.06 0.05 0 0.07 0.06 0.09 0.06 0.09 0 0 0.15 0 0 0 0 0 0.1 0.11 0.1 0.09
SpindlePole 0 0 0 0 0 0 0 0.06 0 0.05 0 0.08 0 0 0.08 0.09 0.06 0.07 0 0 0 0 0 0
Vac/Vac Membrane 0.08 0 0 0 0.05 0 0 0 0 0 0 0 0.06 0.07 0.08 0.09 0.09 0.11 0 0.09 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 12 3 2 1 0 0 5 3 0 2 4 2 7 7 9 9 6 1 7 11 5 10 10
Bud 1 2 11 11 19 39 36 53 52 47 53 42 1 3 3 4 4 3 0 1 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0
Bud Site 0 0 2 0 1 8 8 9 19 15 5 15 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 1 1 1 1 3 4 0 3 3 0 0 0 0 1 2 1 1 0 0 0 0 0 0
Cytoplasm 0 21 15 9 25 23 17 21 19 14 23 13 3 10 13 8 7 11 0 1 0 0 0 1
Endoplasmic Reticulum 2 17 0 2 1 3 5 6 3 2 5 4 4 5 3 4 4 4 0 2 1 0 3 1
Endosome 0 2 12 6 64 31 17 39 8 14 8 20 4 23 47 35 16 23 21 58 38 22 56 48
Golgi 2 13 8 15 9 0 1 2 0 1 0 0 18 53 42 48 61 47 45 86 100 38 182 179
Mitochondria 46 245 168 185 251 543 509 530 444 488 419 423 52 75 41 18 11 10 15 101 27 18 55 45
Nucleus 0 3 1 0 2 1 4 5 1 2 4 1 0 1 2 0 1 0 0 1 0 0 0 0
Nuclear Periphery 0 1 0 0 0 1 3 2 1 2 4 2 0 0 1 1 2 0 0 0 0 0 1 0
Nucleolus 2 0 2 2 1 10 18 28 15 17 21 9 0 1 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 1 4 4 21 31 22 47 29 47 29 43 2 3 23 4 2 3 2 12 20 11 35 27
SpindlePole 0 2 3 4 11 26 25 39 13 25 17 40 1 5 12 10 7 8 1 4 0 0 1 0
Vac/Vac Membrane 4 7 4 7 18 14 5 6 4 5 6 5 4 11 12 10 10 12 1 26 1 2 3 3
Unique Cell Count 48 277 200 205 333 606 570 631 488 550 474 488 71 159 154 116 110 110 92 309 206 101 353 321
Labelled Cell Count 57 327 234 248 425 733 674 792 614 682 596 621 91 197 207 153 135 128 92 309 206 101 353 321


Early Golgi, Punctate Nuclear

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 6.1 5.6 6.3 6.1 6.3 4.8 4.9 5.4 4.8 4.8 4.8 4.7 7.6 7.6 7.8 8.3 8.1 8.1 5.8 7.3 7.2
Std Deviation (1e-4) 1.1 0.8 1.7 1.5 2.0 1.3 1.5 1.6 1.3 1.1 1.6 1.2 1.2 1.0 1.2 1.5 1.4 1.2 0.7 1.0 1.1
Intensity Change (Log2) – – – -0.04 0.0 -0.38 -0.37 -0.23 -0.39 -0.39 -0.39 -0.43 0.27 0.27 0.31 0.39 0.37 0.36 -0.11 0.21 0.2


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud -0.1 0.1 0 0 1.3 0 0 0 0 0 -1.8 -1.7 -0.8 -0.7 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm -1.3 0 -2.1 -2.8 -2.5 -2.0 -3.1 -1.4 -2.9 0 -0.4 0.3 -0.2 -0.4 0.8
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome -1.5 4.2 0 0 0.1 0 0 0 0 0 2.7 6.1 5.8 2.5 4.0
Golgi 1.4 -0.8 0 0 0 0 0 0 0 5.2 7.4 6.2 8.4 10.4 8.5
Mitochondria 1.9 -2.4 0 0 0 0 0 0 0 -2.0 -7.4 -10.9 -11.9 -12.6 -12.8
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 2.3 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 2.3 0 0 2.8 0 0 0 0 0 0 4.5 0 0 0
SpindlePole 0 1.3 0 0 2.6 0 0 0 0 0 0 2.9 0 0 0
Vacuole 0.9 1.9 0 0 0 0 0 0 0 0 2.3 2.6 2.8 0 3.4

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 2.8841 3.2139 3.0016 2.4904 2.5232 2.8991 4.3235 5.2519 4.772 4.2779 4.2306 4.844 3.3282 3.9251 3.5402 3.7322 3.4615 3.612
Actin 0.0324 0.0095 0.0233 0.0011 0.0027 0.0223 0.0427 0.0251 0.0132 0.0025 0.0038 0.0163 0.0298 0.0324 0.0338 0.0048 0.1317 0.022
Bud 0.0012 0.0002 0.0004 0.0002 0 0.0001 0.0004 0.0005 0.0003 0.0001 0.0002 0.0001 0.0004 0.0004 0.0004 0.0001 0.0014 0.0001
Bud Neck 0.0006 0.0004 0.0004 0.0002 0.0002 0.0004 0.0005 0.0005 0.0006 0.0002 0.0003 0.0006 0.0008 0.0004 0.0004 0.0002 0.0003 0.0005
Bud Periphery 0.0004 0.0001 0.0006 0.0002 0 0.0001 0.0003 0.0003 0.0002 0.0001 0.0002 0.0001 0.0003 0.0002 0.0002 0.0001 0.0016 0.0001
Bud Site 0.0021 0.0011 0.0013 0.0004 0.0001 0.0002 0.0015 0.0078 0.0028 0.0001 0.0003 0.0002 0.0015 0.0019 0.0017 0.0001 0.0016 0.0002
Cell Periphery 0.0001 0 0.0001 0 0 0 0.0001 0.0001 0.0001 0 0.0001 0 0.0001 0.0001 0.0001 0 0 0
Cytoplasm 0.0036 0.0054 0.0035 0.0019 0.0017 0.0033 0.0055 0.0169 0.0036 0.0017 0.0021 0.0018 0.0029 0.0334 0.0037 0.001 0.0012 0.002
Cytoplasmic Foci 0.1315 0.1215 0.1074 0.1215 0.1281 0.0752 0.1173 0.1668 0.1281 0.1089 0.1074 0.1036 0.1617 0.1208 0.1159 0.0779 0.0732 0.0895
Eisosomes 0.0001 0 0.0001 0 0 0 0.0001 0.0001 0.0001 0 0 0 0.0001 0 0 0 0.0001 0
Endoplasmic Reticulum 0.0006 0.0005 0.0006 0.0004 0.0003 0.0006 0.0023 0.0007 0.0006 0.0004 0.0006 0.0005 0.0006 0.0008 0.0011 0.0004 0.0004 0.0009
Endosome 0.2355 0.2565 0.2821 0.3204 0.2251 0.2017 0.3185 0.244 0.2987 0.2538 0.3054 0.2107 0.2302 0.1736 0.2934 0.2608 0.267 0.2197
Golgi 0.4952 0.5588 0.5349 0.5285 0.6008 0.6602 0.4304 0.482 0.4939 0.5711 0.5257 0.6218 0.4463 0.6069 0.4906 0.5863 0.4992 0.5949
Lipid Particles 0.0194 0.0032 0.0033 0.0031 0.0114 0.0041 0.0157 0.004 0.0068 0.0064 0.0076 0.0073 0.0336 0.0024 0.0147 0.0042 0.0054 0.0086
Mitochondria 0.0122 0.0111 0.0172 0.0138 0.0031 0.016 0.0258 0.005 0.0194 0.0345 0.0143 0.0124 0.017 0.0064 0.011 0.0194 0.0073 0.0285
None 0.001 0.0001 0.0001 0.0002 0 0.0001 0.0017 0.0004 0.0001 0.0001 0.0001 0.0001 0.0003 0.0027 0.0001 0 0.0001 0.0001
Nuclear Periphery 0.0002 0.0001 0.0002 0.0001 0.0001 0.0001 0.0047 0.0003 0.0003 0.0001 0.0002 0.0001 0.0002 0.0001 0.0002 0.0001 0.0002 0.0002
Nucleolus 0.0004 0 0 0 0 0 0.0002 0.0001 0.0002 0 0 0 0 0 0.0002 0 0 0
Nucleus 0.0006 0.0001 0.0001 0.0001 0 0 0.001 0.0001 0.0004 0 0.0001 0 0.0001 0.0001 0.0001 0 0 0
Peroxisomes 0.0597 0.0284 0.0202 0.0045 0.0245 0.0122 0.0223 0.0388 0.0268 0.0178 0.0281 0.0219 0.0714 0.0151 0.0265 0.0422 0.004 0.0302
Punctate Nuclear 0.0009 0.0001 0.0001 0.0001 0.0003 0.0001 0.0027 0.0026 0.0003 0.0001 0.0001 0.0002 0.0005 0.0002 0.0003 0.0001 0.0001 0.0002
Vacuole 0.0011 0.0015 0.0021 0.0015 0.0005 0.0013 0.0035 0.0025 0.0016 0.0008 0.0016 0.0008 0.0011 0.0012 0.0036 0.0009 0.0025 0.0009
Vacuole Periphery 0.0012 0.0015 0.002 0.0017 0.0008 0.0021 0.0026 0.0013 0.0018 0.0013 0.0019 0.0012 0.0011 0.0008 0.002 0.0015 0.0028 0.0015

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 45.9752 37.3627 34.2779 30.347 35.4697 22.785 27.9896 30.5967 24.5618 35.7954
Translational Efficiency 0.7217 0.655 0.695 0.713 0.6823 1.1873 1.0459 0.7485 0.8954 0.825

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Mu3-like subunit of the clathrin associated protein complex (AP-3); functions in transport of alkaline phosphatase to the vacuole via the alternate pathway
Localization
Cell Percentages Golgi (6%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Apm3

Apm3


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Apm3-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available