Standard name | |
---|---|
Human Ortholog | |
Description | Protein involved in retention of membrane proteins; including Sec12p, in the ER; localized to Golgi; functions as a retrieval receptor in returning membrane proteins to the ER |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 11 | 24 | 11 | 5 | 4 | 4 | 0 | 2 | 0 | 0 | 0 | 3 | 19 | 15 | 4 | 15 | 10 | 14 | 10 | 33 | 14 | 7 | 12 | 11 |
Bud | 2 | 0 | 4 | 8 | 8 | 12 | 27 | 29 | 22 | 34 | 28 | 37 | 1 | 2 | 2 | 1 | 3 | 5 | 1 | 2 | 0 | 6 | 3 | 3 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 1 | 0 | 0 | 0 | 3 | 3 | 1 | 1 | 0 | 2 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 3 | 2 | 5 | 9 | 2 | 5 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 2 | 0 | 0 | 0 | 1 | 2 | 0 | 2 | 2 | 1 | 1 | 4 | 0 | 1 | 0 | 2 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 44 | 81 | 48 | 113 | 171 | 148 | 236 | 232 | 154 | 282 | 162 | 266 | 57 | 88 | 66 | 76 | 52 | 43 | 11 | 25 | 11 | 8 | 12 | 25 |
Endoplasmic Reticulum | 32 | 59 | 34 | 23 | 11 | 12 | 11 | 2 | 1 | 3 | 4 | 19 | 34 | 64 | 13 | 5 | 3 | 8 | 27 | 36 | 27 | 11 | 18 | 27 |
Endosome | 9 | 10 | 13 | 25 | 55 | 8 | 27 | 13 | 4 | 18 | 1 | 0 | 18 | 12 | 34 | 27 | 13 | 26 | 31 | 28 | 29 | 16 | 20 | 16 |
Golgi | 1 | 5 | 3 | 7 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 9 | 3 | 0 | 1 | 1 | 36 | 42 | 34 | 19 | 17 | 15 |
Mitochondria | 5 | 0 | 47 | 65 | 79 | 151 | 298 | 289 | 249 | 296 | 244 | 404 | 1 | 1 | 1 | 10 | 3 | 9 | 30 | 27 | 32 | 35 | 50 | 54 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 3 | 1 | 3 | 2 | 4 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 1 | 0 | 0 | 0 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 2 | 0 | 1 | 1 | 1 | 0 | 1 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 5 | 5 | 0 | 4 | 4 | 2 | 6 | 0 | 2 | 1 | 0 | 1 | 0 | 0 | 0 | 2 | 1 | 5 | 4 | 2 | 2 |
SpindlePole | 1 | 0 | 1 | 7 | 6 | 3 | 14 | 15 | 15 | 26 | 4 | 21 | 2 | 2 | 4 | 8 | 3 | 12 | 2 | 1 | 1 | 0 | 1 | 1 |
Vac/Vac Membrane | 1 | 1 | 0 | 1 | 7 | 0 | 6 | 9 | 4 | 11 | 5 | 5 | 5 | 2 | 11 | 2 | 6 | 8 | 7 | 6 | 2 | 8 | 12 | 15 |
Unique Cell Count | 96 | 162 | 130 | 226 | 303 | 290 | 508 | 491 | 373 | 551 | 356 | 608 | 123 | 178 | 130 | 127 | 83 | 103 | 167 | 213 | 165 | 123 | 157 | 180 |
Labelled Cell Count | 108 | 181 | 161 | 259 | 349 | 341 | 631 | 604 | 461 | 689 | 454 | 770 | 139 | 198 | 141 | 146 | 94 | 127 | 167 | 213 | 165 | 123 | 157 | 180 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 6.4 | 8.1 | 5.6 | 5.0 | 5.3 | 4.3 | 3.9 | 3.8 | 3.4 | 3.5 | 3.2 | 3.3 | 7.2 | 7.2 | 7.2 | 7.3 | 7.8 | 7.3 | 7.9 | 8.0 | 7.3 |
Std Deviation (1e-4) | 1.0 | 1.6 | 1.2 | 1.3 | 2.0 | 1.2 | 1.3 | 1.2 | 0.9 | 0.9 | 0.8 | 0.7 | 1.5 | 1.4 | 1.3 | 1.2 | 1.4 | 1.3 | 1.8 | 1.8 | 1.4 |
Intensity Change (Log2) | – | – | – | -0.16 | -0.08 | -0.37 | -0.53 | -0.57 | -0.69 | -0.69 | -0.79 | -0.75 | 0.36 | 0.38 | 0.36 | 0.4 | 0.49 | 0.39 | 0.5 | 0.52 | 0.4 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | -2.7 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1.7 | 0 | -1.9 | 0.9 | 0.9 | 1.3 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 2.4 | 3.7 | 2.7 | 2.0 | 2.1 | 0.9 | 2.9 | 1.7 | 1.4 | 1.5 | 2.2 | 2.2 | 3.7 | 3.7 | 0.7 |
Endoplasmic Reticulum | -4.0 | -7.0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0.3 | 1.8 | -3.4 | -5.0 | -4.2 | -3.6 |
Endosome | 0.3 | 2.1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1.1 | -1.0 | 3.4 | 2.5 | 1.2 | 3.1 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1.2 | 0 | 0 | 0 | 0 |
Mitochondria | -1.4 | -2.1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | -7.2 | -8.5 | -7.4 | -5.5 | -5.5 | -4.9 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3.6 |
Vacuole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3.4 | 0 | 0 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 2.4874 | 3.01 | 3.0138 | 2.3508 | 2.4259 | 2.9063 | 3.3991 | 4.8694 | 4.0284 | 4.1966 | 4.2895 | 3.9486 | 1.2437 | 2.7431 | 2.3331 | 2.1607 | 2.1103 | 1.9772 |
Actin | 0.0559 | 0.0117 | 0.024 | 0.0387 | 0.0347 | 0.0313 | 0.0613 | 0.0805 | 0.062 | 0.0013 | 0.0342 | 0.0287 | 0.0215 | 0.0059 | 0.0158 | 0.0075 | 0.0043 | 0.0155 |
Bud | 0.0006 | 0.0008 | 0.0006 | 0.0004 | 0.0006 | 0.0004 | 0.0005 | 0.0004 | 0.0009 | 0.0005 | 0.008 | 0.0005 | 0.0004 | 0.0006 | 0.0005 | 0.0008 | 0.0003 | 0.0015 |
Bud Neck | 0.0014 | 0.0006 | 0.0009 | 0.0007 | 0.0012 | 0.0047 | 0.0022 | 0.001 | 0.0064 | 0.0001 | 0.0003 | 0.0035 | 0.0029 | 0.0004 | 0.0008 | 0.0005 | 0.0005 | 0.0019 |
Bud Periphery | 0.0008 | 0.0006 | 0.0005 | 0.0002 | 0.0006 | 0.0008 | 0.0005 | 0.0005 | 0.001 | 0.0001 | 0.0243 | 0.0013 | 0.0006 | 0.0005 | 0.0007 | 0.002 | 0.0003 | 0.0006 |
Bud Site | 0.0057 | 0.0048 | 0.0056 | 0.0006 | 0.0018 | 0.0009 | 0.0258 | 0.0088 | 0.0035 | 0.0004 | 0.0025 | 0.0006 | 0.0043 | 0.0028 | 0.0027 | 0.0008 | 0.0004 | 0.0005 |
Cell Periphery | 0.0006 | 0.0004 | 0.0004 | 0.0002 | 0.0003 | 0.0004 | 0.0017 | 0.002 | 0.0002 | 0 | 0.0004 | 0.0003 | 0.0005 | 0.0002 | 0.0004 | 0.0006 | 0.0001 | 0.0002 |
Cytoplasm | 0.076 | 0.1815 | 0.1279 | 0.0371 | 0.0301 | 0.0558 | 0.0577 | 0.0474 | 0.0589 | 0.0592 | 0.0405 | 0.0855 | 0.0644 | 0.0646 | 0.0512 | 0.0787 | 0.0483 | 0.0747 |
Cytoplasmic Foci | 0.1725 | 0.2028 | 0.1681 | 0.138 | 0.1759 | 0.1715 | 0.1671 | 0.2525 | 0.202 | 0.1158 | 0.1563 | 0.1677 | 0.1798 | 0.1885 | 0.1727 | 0.153 | 0.1973 | 0.1895 |
Eisosomes | 0.0003 | 0.0001 | 0.0002 | 0.0002 | 0.0006 | 0.0006 | 0.0005 | 0.0014 | 0.0001 | 0 | 0.0005 | 0.0002 | 0.0003 | 0.0001 | 0.0002 | 0.0001 | 0.0001 | 0.0001 |
Endoplasmic Reticulum | 0.0262 | 0.0229 | 0.0303 | 0.0099 | 0.0305 | 0.0179 | 0.023 | 0.0083 | 0.0234 | 0.0122 | 0.0202 | 0.0116 | 0.0138 | 0.0099 | 0.0082 | 0.0285 | 0.017 | 0.0093 |
Endosome | 0.2994 | 0.2836 | 0.309 | 0.4887 | 0.3207 | 0.2988 | 0.2151 | 0.2567 | 0.2819 | 0.3835 | 0.3469 | 0.2365 | 0.3285 | 0.3624 | 0.34 | 0.3564 | 0.2912 | 0.3367 |
Golgi | 0.2216 | 0.2092 | 0.26 | 0.253 | 0.2908 | 0.3257 | 0.2503 | 0.2352 | 0.2861 | 0.4085 | 0.1801 | 0.3366 | 0.2608 | 0.2805 | 0.3173 | 0.251 | 0.3023 | 0.283 |
Lipid Particles | 0.0257 | 0.0136 | 0.0097 | 0.0097 | 0.0132 | 0.0178 | 0.0307 | 0.0122 | 0.0154 | 0.0056 | 0.0137 | 0.0295 | 0.0375 | 0.0187 | 0.0187 | 0.0222 | 0.0197 | 0.0167 |
Mitochondria | 0.0215 | 0.0128 | 0.0222 | 0.0126 | 0.0169 | 0.0188 | 0.0115 | 0.0056 | 0.0097 | 0.0054 | 0.0326 | 0.0216 | 0.0218 | 0.0172 | 0.0291 | 0.0587 | 0.0102 | 0.0193 |
None | 0.0533 | 0.0303 | 0.0206 | 0.0009 | 0.0521 | 0.0249 | 0.069 | 0.0678 | 0.0133 | 0.0003 | 0.0894 | 0.0292 | 0.0306 | 0.0279 | 0.0134 | 0.007 | 0.0461 | 0.0322 |
Nuclear Periphery | 0.0027 | 0.0022 | 0.0022 | 0.0006 | 0.0045 | 0.0007 | 0.0052 | 0.0008 | 0.0013 | 0.0008 | 0.0098 | 0.0006 | 0.0015 | 0.0006 | 0.0024 | 0.0063 | 0.0214 | 0.0014 |
Nucleolus | 0.0012 | 0.0001 | 0.0001 | 0 | 0.0009 | 0.0001 | 0.0014 | 0.0001 | 0.0005 | 0 | 0.0006 | 0.0001 | 0.0043 | 0.0001 | 0.0001 | 0.0002 | 0.0004 | 0.0001 |
Nucleus | 0.002 | 0.0006 | 0.0005 | 0.0001 | 0.0015 | 0.0002 | 0.0132 | 0.0002 | 0.0006 | 0.0001 | 0.0018 | 0.0003 | 0.0017 | 0.0004 | 0.0004 | 0.0064 | 0.0015 | 0.0004 |
Peroxisomes | 0.0179 | 0.0137 | 0.0117 | 0.0031 | 0.0109 | 0.0246 | 0.0535 | 0.0151 | 0.0255 | 0.0014 | 0.0243 | 0.0415 | 0.0181 | 0.014 | 0.0194 | 0.0099 | 0.0245 | 0.0106 |
Punctate Nuclear | 0.0086 | 0.0008 | 0.0006 | 0.0002 | 0.0067 | 0.0004 | 0.0017 | 0.0006 | 0.0023 | 0.0003 | 0.0073 | 0.0007 | 0.0023 | 0.0004 | 0.0007 | 0.0024 | 0.0049 | 0.0009 |
Vacuole | 0.004 | 0.005 | 0.0029 | 0.0029 | 0.0027 | 0.0018 | 0.0065 | 0.0021 | 0.0028 | 0.0021 | 0.0032 | 0.002 | 0.0027 | 0.0028 | 0.0031 | 0.0044 | 0.0041 | 0.0028 |
Vacuole Periphery | 0.0018 | 0.0019 | 0.0018 | 0.0022 | 0.0028 | 0.0018 | 0.0017 | 0.0009 | 0.0019 | 0.0022 | 0.003 | 0.0015 | 0.0016 | 0.0015 | 0.0023 | 0.0025 | 0.005 | 0.0021 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 90.4908 | 48.7099 | 47.7629 | 58.4821 | 61.8166 | 38.8992 | 76.5143 | 66.4816 | 53.9134 | 78.4236 |
Translational Efficiency | 1.9373 | 3.158 | 2.115 | 1.456 | 1.9313 | 4.4396 | 1.7812 | 1.4781 | 1.6172 | 1.8059 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✔ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
1174 | 1290 | 1660 | 1419 | 1804 | 1725 | 2424 | 1544 | 2978 | 3015 | 4084 | 2963 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 694.27 | 783.09 | 1033.39 | 1197.88 | 838.43 | 791.62 | 1110.82 | 1145.49 | 781.60 | 787.97 | 1079.35 | 1170.58 |
Standard Deviation | 88.87 | 146.46 | 154.57 | 179.39 | 104.23 | 109.97 | 146.87 | 196.89 | 121.07 | 126.94 | 154.79 | 190.52 |
Intensity Change Log 2 | — | 0.173681 | 0.573816 | 0.786915 | — | -0.082882 | 0.405863 | 0.450203 | — | 0.039011 | 0.484371 | 0.612509 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.001880 | 0.007734 | 0.035768 | 0.060009 | 0.006029 | 0.009792 | 0.050182 | 0.065677 | 0.004393 | 0.008911 | 0.044323 | 0.062963 |
Bud Neck | 0.010975 | 0.002422 | 0.001657 | 0.007153 | 0.002150 | 0.004279 | 0.004556 | 0.007121 | 0.005629 | 0.003484 | 0.003378 | 0.007136 |
Bud Site | 0.007452 | 0.007599 | 0.015211 | 0.052144 | 0.004444 | 0.007299 | 0.025308 | 0.048083 | 0.005630 | 0.007427 | 0.021204 | 0.050028 |
Cell Periphery | 0.000723 | 0.000216 | 0.000247 | 0.001734 | 0.000288 | 0.000228 | 0.000434 | 0.002113 | 0.000459 | 0.000223 | 0.000358 | 0.001932 |
Cytoplasm | 0.135294* | 0.040701 | 0.024191 | 0.073017 | 0.056317 | 0.047663 | 0.078968 | 0.083197 | 0.087452 | 0.044685 | 0.056703 | 0.078322 |
Cytoplasmic Foci | 0.306734* | 0.321701* | 0.302699* | 0.200309* | 0.298445* | 0.283582* | 0.221482* | 0.210567* | 0.301713* | 0.299892* | 0.254494* | 0.205654* |
Eisosomes | 0.001379 | 0.000513 | 0.000231 | 0.000849 | 0.000980 | 0.001270 | 0.000738 | 0.001797 | 0.001137 | 0.000946 | 0.000532 | 0.001343 |
Endoplasmic Reticulum | 0.045097 | 0.014971 | 0.060578 | 0.070115 | 0.027916 | 0.012316 | 0.099649 | 0.077084 | 0.034689 | 0.013452 | 0.083768 | 0.073746 |
Endosome | 0.082766 | 0.152284 | 0.133963 | 0.110247 | 0.073718 | 0.126876 | 0.085593 | 0.103024 | 0.077285 | 0.137747 | 0.105253 | 0.106483 |
Golgi | 0.150427* | 0.285301* | 0.241198* | 0.100296 | 0.375951* | 0.337793* | 0.144788* | 0.130380* | 0.287044* | 0.315334* | 0.183975* | 0.115972* |
Lipid Particles | 0.140070* | 0.086771 | 0.117470* | 0.185453* | 0.103912* | 0.096960 | 0.207114* | 0.174895* | 0.118166* | 0.092601 | 0.170677* | 0.179951* |
Mitochondria | 0.031817 | 0.016598 | 0.001464 | 0.002059 | 0.015719 | 0.020379 | 0.002802 | 0.003651 | 0.022065 | 0.018761 | 0.002258 | 0.002889 |
Mitotic Spindle | 0.000725 | 0.004658 | 0.025878 | 0.028433 | 0.001568 | 0.001619 | 0.013684 | 0.007902 | 0.001236 | 0.002919 | 0.018640 | 0.017735 |
None | 0.001440 | 0.001441 | 0.000079 | 0.000206 | 0.000250 | 0.000227 | 0.000256 | 0.000108 | 0.000719 | 0.000747 | 0.000184 | 0.000155 |
Nuclear Periphery | 0.000957 | 0.000875 | 0.000300 | 0.000353 | 0.000179 | 0.000163 | 0.000369 | 0.000232 | 0.000486 | 0.000468 | 0.000341 | 0.000290 |
Nuclear Periphery Foci | 0.001227 | 0.004729 | 0.012212 | 0.011601 | 0.001029 | 0.000821 | 0.010358 | 0.004312 | 0.001107 | 0.002493 | 0.011112 | 0.007803 |
Nucleolus | 0.000790 | 0.001985 | 0.000029 | 0.000063 | 0.000100 | 0.000253 | 0.000095 | 0.000070 | 0.000372 | 0.000994 | 0.000068 | 0.000067 |
Nucleus | 0.009339 | 0.003564 | 0.000674 | 0.002633 | 0.002724 | 0.002376 | 0.004191 | 0.002757 | 0.005332 | 0.002884 | 0.002761 | 0.002697 |
Peroxisomes | 0.021698 | 0.012006 | 0.004331 | 0.014199 | 0.014326 | 0.024164 | 0.010220 | 0.018844 | 0.017232 | 0.018962 | 0.007826 | 0.016620 |
Vacuole | 0.039437 | 0.026036 | 0.019787 | 0.076330 | 0.009876 | 0.015791 | 0.037401 | 0.054684 | 0.021530 | 0.020175 | 0.030241 | 0.065050 |
Vacuole Periphery | 0.009772 | 0.007895 | 0.002032 | 0.002794 | 0.004077 | 0.006149 | 0.001815 | 0.003503 | 0.006322 | 0.006896 | 0.001903 | 0.003164 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | -5.43 | -13.54 | -15.35 | -13.17 | -4.73 | -3.05 | -16.71 | -15.92 | -14.40 | -3.44 | -5.31 | -21.34 | -21.91 | -19.45 | -5.38 |
Bud Neck | 7.23 | 7.88 | 2.57 | -9.88 | -11.47 | -4.28 | -6.68 | -9.74 | -5.09 | -4.55 | 3.83 | 3.78 | -3.21 | -9.81 | -10.34 |
Bud Site | -0.09 | -5.76 | -14.75 | -14.58 | -12.10 | -3.27 | -13.74 | -15.13 | -13.88 | -6.99 | -2.32 | -14.76 | -21.36 | -20.16 | -12.79 |
Cell Periphery | 4.54 | 4.45 | -2.67 | -4.44 | -4.36 | 1.22 | -3.04 | -6.01 | -6.27 | -5.54 | 4.43 | 1.91 | -6.32 | -7.58 | -6.94 |
Cytoplasm | 16.56 | 20.63 | 8.60 | -8.77 | -13.52 | 2.36 | -6.98 | -6.87 | -8.98 | -0.71 | 13.23 | 8.27 | 0.79 | -12.44 | -7.45 |
Cytoplasmic Foci | -1.94 | 0.83 | 16.29 | 19.72 | 17.92 | 2.49 | 15.04 | 14.90 | 12.35 | 1.67 | 0.40 | 11.60 | 22.29 | 22.30 | 12.51 |
Eisosomes | 7.18 | 10.15 | 4.49 | -3.14 | -7.44 | -1.67 | 2.37 | -3.65 | -2.04 | -5.60 | 1.70 | 7.68 | -1.09 | -2.55 | -7.43 |
Endoplasmic Reticulum | 7.16 | -3.06 | -3.42 | -10.55 | -0.63 | 5.88 | -15.81 | -9.63 | -13.87 | 4.13 | 9.16 | -14.28 | -9.62 | -17.40 | 3.27 |
Endosome | -12.25 | -12.13 | -4.32 | 8.86 | 7.53 | -13.43 | -3.77 | -7.60 | 5.93 | -4.97 | -18.25 | -11.30 | -8.69 | 10.37 | 1.04 |
Golgi | -15.41 | -12.21 | 9.34 | 25.64 | 25.35* | 4.47 | 34.53 | 34.39 | 29.30 | 2.72 | -4.24 | 19.61 | 32.85 | 39.21 | 18.31 |
Lipid Particles | 7.92 | 3.32 | -5.14 | -12.93 | -8.68 | 1.37 | -17.18 | -10.30 | -11.44 | 4.72 | 6.23 | -11.52 | -11.49 | -17.17 | -1.13 |
Mitochondria | 6.00 | 14.75 | 14.27 | 9.28 | -0.70 | -2.86 | 12.74 | 9.56 | 10.97 | -0.83 | 2.34 | 19.28 | 17.24 | 14.58 | -0.90 |
Mitotic Spindle | -4.91 | -11.10 | -10.71 | -9.02 | -1.29 | -0.12 | -10.67 | -4.99 | -5.02 | 3.76 | -3.73 | -15.26 | -11.63 | -10.36 | 0.18 |
None | 0.00 | 3.68 | 3.26 | 3.06 | -1.24 | 0.41 | -0.02 | 3.47 | 2.23 | 1.19 | -0.12 | 3.32 | 3.66 | 3.34 | 0.33 |
Nuclear Periphery | 0.26 | 4.03 | 3.00 | 1.66 | -2.19 | 0.34 | -4.47 | -1.07 | -1.53 | 2.77 | 0.13 | 1.39 | 1.74 | 1.04 | 0.73 |
Nuclear Periphery Foci | -3.65 | -9.92 | -10.77 | -5.93 | -0.85 | 0.82 | -12.06 | -8.04 | -8.77 | 7.17 | -3.20 | -15.61 | -12.98 | -8.90 | 3.18 |
Nucleolus | -2.02 | 3.02 | 2.86 | 3.51 | -4.56 | -1.71 | -0.21 | 1.91 | 2.01 | 2.28 | -2.42 | 2.99 | 3.00 | 3.84 | 0.06 |
Nucleus | 5.04 | 10.01 | 4.17 | -1.07 | -5.83 | 0.50 | -3.77 | -1.17 | -1.60 | 2.84 | 3.98 | 4.42 | 2.27 | -1.92 | -1.92 |
Peroxisomes | 6.84 | 14.54 | 5.36 | -0.98 | -7.47 | -6.15 | 3.98 | -3.34 | 3.31 | -7.35 | -1.56 | 11.94 | 1.11 | 2.47 | -9.84 |
Vacuole | 3.75 | 6.29 | -12.47 | -15.54 | -17.75 | -4.40 | -17.86 | -16.44 | -14.27 | -5.94 | 0.70 | -7.89 | -20.74 | -21.19 | -16.48 |
Vacuole Periphery | 2.03 | 11.73 | 9.55 | 6.85 | -1.23 | -3.63 | 8.02 | 1.22 | 3.80 | -2.91 | -1.12 | 14.02 | 7.42 | 7.41 | -3.13 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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