Standard name | |
---|---|
Human Ortholog | |
Description | S-phase checkpoint protein required for DNA replication; couples DNA helicase and polymerase; interacts with and stabilizes Pol2p at stalled replication forks during stress, where it forms a pausing complex with Tof1p and is phosphorylated by Mec1p; defines a novel S-phase checkpoint with Hog1p that coordinates DNA replication and transcription upon osmostress; protects uncapped telomeres; Dia2p-dependent degradation mediates checkpoint recovery; mammalian claspin homolog |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 1 |
Bud | 0 | 0 | 0 | 0 | 0 | 3 | 3 | 2 | 4 | – | 8 | 4 | 0 | 1 | 1 | 0 | 0 | 0 | 4 | 6 | 4 | 1 | 5 | 5 |
Bud Neck | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 0 |
Bud Site | 0 | 0 | 2 | 1 | 4 | 4 | 3 | 17 | 6 | – | 15 | 9 | 0 | 0 | 2 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 3 | 16 | 10 | 13 | 17 | 10 | 15 | 19 | 12 | – | 10 | 5 | 29 | 56 | 55 | 24 | 15 | 24 | 0 | 4 | 2 | 3 | 5 | 3 |
Endoplasmic Reticulum | 3 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 6 | 2 | 7 | 0 | 0 | 0 | 0 | 2 | 0 |
Endosome | 0 | 1 | 1 | 0 | 2 | 0 | 0 | 0 | 0 | – | 0 | 0 | 1 | 2 | 0 | 8 | 5 | 0 | 1 | 3 | 1 | 1 | 2 | 22 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 2 | 3 | 1 | 0 | 2 |
Mitochondria | 1 | 36 | 56 | 100 | 58 | 133 | 201 | 218 | 200 | – | 184 | 213 | 18 | 6 | 59 | 11 | 5 | 11 | 1 | 4 | 1 | 1 | 3 | 10 |
Nucleus | 97 | 371 | 245 | 199 | 309 | 186 | 307 | 381 | 211 | – | 182 | 190 | 195 | 246 | 228 | 225 | 148 | 169 | 106 | 359 | 231 | 171 | 164 | 133 |
Nuclear Periphery | 0 | 0 | 0 | 1 | 4 | 2 | 9 | 7 | 1 | – | 3 | 2 | 1 | 2 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 3 | 5 | 1 | 3 | 8 | 10 | 8 | 18 | 8 | – | 6 | 12 | 11 | 9 | 14 | 4 | 4 | 3 | 2 | 3 | 6 | 0 | 3 | 17 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 1 | 0 | 2 | 3 | 3 | 0 | 3 | 4 | 1 | – | 1 | 1 | 1 | 2 | 0 | 2 | 5 | 1 | 2 | 2 | 5 | 2 | 11 | 45 |
Vac/Vac Membrane | 0 | 19 | 3 | 2 | 11 | 4 | 4 | 3 | 4 | – | 4 | 6 | 4 | 5 | 8 | 19 | 14 | 11 | 6 | 36 | 28 | 20 | 44 | 58 |
Unique Cell Count | 103 | 414 | 271 | 240 | 359 | 246 | 392 | 471 | 283 | 258 | 286 | 231 | 310 | 315 | 270 | 179 | 205 | 128 | 430 | 287 | 208 | 249 | 313 | |
Labelled Cell Count | 108 | 448 | 322 | 322 | 417 | 352 | 553 | 669 | 447 | 413 | 442 | 260 | 329 | 368 | 299 | 200 | 227 | 128 | 430 | 287 | 208 | 249 | 313 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 5.9 | 5.1 | 4.3 | 4.2 | 4.5 | 3.9 | 3.9 | 3.8 | 3.3 | – | 3.2 | 3.0 | 5.3 | 5.3 | 4.5 | 6.7 | 7.2 | 7.2 | 5.1 | 5.1 | 5.6 |
Std Deviation (1e-4) | 0.7 | 1.0 | 0.8 | 1.0 | 1.3 | 0.9 | 1.3 | 1.0 | 0.7 | – | 0.6 | 0.7 | 1.3 | 1.3 | 1.1 | 1.5 | 1.7 | 1.9 | 1.0 | 1.5 | 1.5 |
Intensity Change (Log2) | – | – | – | -0.03 | 0.09 | -0.14 | -0.11 | -0.18 | -0.37 | – | -0.41 | -0.5 | 0.32 | 0.32 | 0.09 | 0.64 | 0.75 | 0.75 | 0.27 | 0.27 | 0.39 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 0.9 | 0.6 | 0.2 | 0.1 | 0.2 | 0.3 | – | 0.1 | -1.4 | 3.7 | 5.4 | 5.3 | 2.5 | 2.1 | 3.4 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | -4.1 | -7.3 | 0 | -5.9 | -5.4 | -4.8 |
Nucleus | -2.5 | -1.7 | -4.5 | -4.1 | -3.4 | -4.9 | – | -5.8 | -6.8 | -2.0 | -3.7 | -5.5 | -2.4 | -2.4 | -2.6 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 3.2 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | 1.6 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 1.3 | 3.5 | 3.7 | 2.7 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 4.0547 | 2.9082 | 2.8522 | 2.9393 | 2.1249 | 2.645 | 5.1252 | 3.6242 | 3.7745 | 3.6283 | 3.3681 | 3.4137 | 3.9913 | 4.1686 | 4.2223 | 4.5004 | 3.8035 | 4.0604 |
Actin | 0.1428 | 0 | 0.0208 | 0.0409 | 0.0053 | 0.0004 | 0.0601 | 0 | 0.0096 | 0.0259 | 0.0155 | 0.002 | 0.0272 | 0 | 0.0005 | 0.0001 | 0 | 0.0003 |
Bud | 0.0015 | 0 | 0.0002 | 0.0004 | 0.0007 | 0.0012 | 0.0013 | 0.0001 | 0.0005 | 0.0013 | 0.0129 | 0.0006 | 0.0001 | 0 | 0.0001 | 0 | 0 | 0.0002 |
Bud Neck | 0.0161 | 0.0002 | 0.0005 | 0.0005 | 0.001 | 0.0015 | 0.0156 | 0.0003 | 0.0051 | 0.0155 | 0.0167 | 0.0022 | 0.0006 | 0.0003 | 0.0002 | 0.0001 | 0.0007 | 0.001 |
Bud Periphery | 0.0032 | 0 | 0.0003 | 0.0003 | 0.0013 | 0.0005 | 0.0033 | 0.0001 | 0.0007 | 0.0027 | 0.0388 | 0.0009 | 0.0004 | 0 | 0.0002 | 0 | 0 | 0.0007 |
Bud Site | 0.0091 | 0.0002 | 0.0013 | 0.0021 | 0.0024 | 0.0004 | 0.0228 | 0.0002 | 0.008 | 0.0102 | 0.0272 | 0.0006 | 0.0004 | 0.0004 | 0.0004 | 0 | 0.0001 | 0.0002 |
Cell Periphery | 0.0019 | 0 | 0.0001 | 0 | 0.0004 | 0.0001 | 0.0008 | 0 | 0.0004 | 0.0007 | 0.0011 | 0.0001 | 0.0002 | 0 | 0.0001 | 0 | 0 | 0 |
Cytoplasm | 0.0264 | 0.0011 | 0.0026 | 0.004 | 0.0026 | 0.0027 | 0.0329 | 0.001 | 0.0018 | 0.0032 | 0.0015 | 0.0035 | 0.0215 | 0.0048 | 0.0004 | 0.0027 | 0.0006 | 0.0093 |
Cytoplasmic Foci | 0.0282 | 0 | 0.0023 | 0.0206 | 0.0124 | 0.0077 | 0.0122 | 0.0001 | 0.0068 | 0.0051 | 0.0017 | 0.0009 | 0.0039 | 0.0001 | 0 | 0.0001 | 0 | 0.0004 |
Eisosomes | 0.0031 | 0 | 0.0005 | 0.0002 | 0.0001 | 0 | 0.0007 | 0 | 0.0001 | 0.0003 | 0.0001 | 0 | 0.0001 | 0 | 0 | 0 | 0 | 0 |
Endoplasmic Reticulum | 0.0155 | 0.0001 | 0.0045 | 0.0002 | 0.0007 | 0.0005 | 0.0099 | 0.0001 | 0.0014 | 0.0014 | 0.0014 | 0.0038 | 0.0032 | 0.0003 | 0.0003 | 0.0001 | 0.0001 | 0.0003 |
Endosome | 0.0494 | 0.0001 | 0.0117 | 0.0035 | 0.0307 | 0.0034 | 0.0222 | 0.0001 | 0.0109 | 0.0384 | 0.0057 | 0.0084 | 0.0111 | 0.0007 | 0.0002 | 0.0001 | 0.0001 | 0.0005 |
Golgi | 0.0237 | 0 | 0.0092 | 0.0059 | 0.0214 | 0.0007 | 0.0105 | 0 | 0.0064 | 0.0068 | 0.0032 | 0.002 | 0.0024 | 0 | 0 | 0 | 0 | 0 |
Lipid Particles | 0.0247 | 0 | 0.0019 | 0.01 | 0.016 | 0.0023 | 0.0049 | 0 | 0.0063 | 0.0058 | 0.0011 | 0.0004 | 0.0121 | 0 | 0 | 0 | 0 | 0.0004 |
Mitochondria | 0.0121 | 0.0001 | 0.0035 | 0.0015 | 0.0348 | 0.0011 | 0.0138 | 0.0002 | 0.0047 | 0.0237 | 0.0048 | 0.0055 | 0.0067 | 0.0001 | 0.0015 | 0.0001 | 0.0002 | 0.0021 |
None | 0.0342 | 0.0001 | 0.0011 | 0.0065 | 0.0039 | 0.0031 | 0.0172 | 0.0001 | 0.0004 | 0.0005 | 0.0003 | 0.0009 | 0.008 | 0.0094 | 0.0002 | 0.0281 | 0.0001 | 0.0055 |
Nuclear Periphery | 0.0394 | 0.0028 | 0.0081 | 0.0009 | 0.0047 | 0.0058 | 0.0304 | 0.0039 | 0.013 | 0.0043 | 0.0033 | 0.0132 | 0.0265 | 0.0055 | 0.005 | 0.0023 | 0.0022 | 0.0019 |
Nucleolus | 0.0134 | 0.0102 | 0.0074 | 0.0066 | 0.019 | 0.0206 | 0.0129 | 0.0103 | 0.0115 | 0.0043 | 0.0106 | 0.0184 | 0.0142 | 0.0109 | 0.0087 | 0.0054 | 0.0186 | 0.0167 |
Nucleus | 0.4977 | 0.9815 | 0.9051 | 0.8582 | 0.7722 | 0.9392 | 0.6896 | 0.9638 | 0.8879 | 0.7925 | 0.84 | 0.9296 | 0.8283 | 0.9491 | 0.9805 | 0.9264 | 0.9377 | 0.9565 |
Peroxisomes | 0.0225 | 0 | 0.0006 | 0.0195 | 0.0095 | 0.0006 | 0.0042 | 0 | 0.0015 | 0.0178 | 0.0007 | 0.0004 | 0.0021 | 0 | 0 | 0 | 0 | 0.0002 |
Punctate Nuclear | 0.0221 | 0.0029 | 0.0161 | 0.0181 | 0.05 | 0.0072 | 0.0195 | 0.018 | 0.0195 | 0.0028 | 0.0013 | 0.0036 | 0.0142 | 0.0137 | 0.0007 | 0.0341 | 0.0395 | 0.0032 |
Vacuole | 0.0091 | 0.0004 | 0.0014 | 0.0002 | 0.0077 | 0.0009 | 0.0076 | 0.0014 | 0.0026 | 0.0296 | 0.0094 | 0.0016 | 0.0113 | 0.0038 | 0.0005 | 0.0001 | 0.0001 | 0.0006 |
Vacuole Periphery | 0.0039 | 0.0001 | 0.0007 | 0.0002 | 0.0031 | 0.0003 | 0.0076 | 0.0003 | 0.0008 | 0.0072 | 0.0025 | 0.0011 | 0.0054 | 0.0005 | 0.0004 | 0.0001 | 0 | 0.0002 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 26.99 | 8.3921 | 11.7874 | 16.5219 | 27.3864 | 30.4935 | 10.9097 | 7.9769 | 12.9721 | 27.8428 |
Translational Efficiency | 1.981 | 1.451 | 0.7761 | 1.1595 | 1.2746 | 1.537 | 0.9082 | 0.9811 | 1.02 | 1.0847 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✔ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
1525 | 1435 | 1595 | 148 | 1436 | 1723 | 167 | 1275 | 2961 | 3158 | 1762 | 1423 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 590.86 | 791.96 | 979.03 | 957.30 | 668.14 | 767.66 | 992.86 | 862.08 | 628.34 | 778.70 | 980.34 | 871.98 |
Standard Deviation | 73.61 | 113.24 | 130.49 | 117.09 | 129.62 | 92.35 | 101.33 | 125.60 | 111.49 | 103.08 | 128.08 | 128.08 |
Intensity Change Log 2 | — | 0.422611 | 0.728537 | 0.696155 | — | 0.200317 | 0.571440 | 0.367671 | — | 0.308916 | 0.647301 | 0.531169 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.000066 | 0.000295 | 0.000380 | 0.001936 | 0.000078 | 0.000179 | 0.000875 | 0.001364 | 0.000072 | 0.000232 | 0.000427 | 0.001424 |
Bud Neck | 0.003834 | 0.005525 | 0.009203 | 0.008176 | 0.002280 | 0.005073 | 0.003823 | 0.024963 | 0.003080 | 0.005278 | 0.008693 | 0.023217 |
Bud Site | 0.001464 | 0.001301 | 0.002094 | 0.005542 | 0.000482 | 0.001888 | 0.001147 | 0.020345 | 0.000988 | 0.001621 | 0.002005 | 0.018805 |
Cell Periphery | 0.000282 | 0.000149 | 0.000083 | 0.000331 | 0.000632 | 0.000115 | 0.000073 | 0.000396 | 0.000452 | 0.000131 | 0.000082 | 0.000389 |
Cytoplasm | 0.146581* | 0.031179 | 0.016682 | 0.049209 | 0.054570 | 0.040236 | 0.004078 | 0.054411 | 0.101958* | 0.036120 | 0.015488 | 0.053870 |
Cytoplasmic Foci | 0.017233 | 0.005664 | 0.000092 | 0.011307 | 0.007793 | 0.005023 | 0.000116 | 0.006581 | 0.012655 | 0.005314 | 0.000095 | 0.007072 |
Eisosomes | 0.000036 | 0.000022 | 0.000013 | 0.000082 | 0.000882 | 0.000021 | 0.000042 | 0.000043 | 0.000446 | 0.000021 | 0.000015 | 0.000047 |
Endoplasmic Reticulum | 0.000701 | 0.004037 | 0.004406 | 0.007020 | 0.002000 | 0.003133 | 0.009193 | 0.006888 | 0.001331 | 0.003544 | 0.004860 | 0.006902 |
Endosome | 0.000809 | 0.000689 | 0.000207 | 0.016820 | 0.000642 | 0.001344 | 0.000377 | 0.009546 | 0.000728 | 0.001047 | 0.000223 | 0.010302 |
Golgi | 0.000182 | 0.000211 | 0.000039 | 0.002065 | 0.000312 | 0.000667 | 0.000060 | 0.003043 | 0.000245 | 0.000459 | 0.000041 | 0.002941 |
Lipid Particles | 0.002006 | 0.001323 | 0.000100 | 0.009665 | 0.003026 | 0.002256 | 0.000329 | 0.001827 | 0.002501 | 0.001832 | 0.000121 | 0.002642 |
Mitochondria | 0.000879 | 0.002222 | 0.000854 | 0.011355 | 0.001104 | 0.004978 | 0.000727 | 0.008952 | 0.000988 | 0.003726 | 0.000842 | 0.009201 |
Mitotic Spindle | 0.000252 | 0.002859 | 0.000758 | 0.033895 | 0.001504 | 0.005890 | 0.000266 | 0.064585 | 0.000859 | 0.004513 | 0.000711 | 0.061393 |
None | 0.048433 | 0.009141 | 0.002338 | 0.014042 | 0.038362 | 0.005488 | 0.004671 | 0.013026 | 0.043549 | 0.007148 | 0.002559 | 0.013131 |
Nuclear Periphery | 0.000558 | 0.001306 | 0.000661 | 0.001795 | 0.000989 | 0.002464 | 0.000594 | 0.002158 | 0.000767 | 0.001938 | 0.000654 | 0.002120 |
Nuclear Periphery Foci | 0.000573 | 0.000428 | 0.000186 | 0.002043 | 0.000838 | 0.000552 | 0.000414 | 0.002983 | 0.000701 | 0.000496 | 0.000207 | 0.002886 |
Nucleolus | 0.014495 | 0.009259 | 0.000756 | 0.009986 | 0.009250 | 0.008067 | 0.005062 | 0.008523 | 0.011951 | 0.008609 | 0.001164 | 0.008675 |
Nucleus | 0.758018* | 0.921525* | 0.957668* | 0.791323* | 0.873320* | 0.906435* | 0.963954* | 0.736641* | 0.813936* | 0.913292* | 0.958264* | 0.742328* |
Peroxisomes | 0.000794 | 0.001008 | 0.000054 | 0.009969 | 0.000687 | 0.001422 | 0.000249 | 0.001780 | 0.000742 | 0.001234 | 0.000072 | 0.002631 |
Vacuole | 0.002666 | 0.001611 | 0.002785 | 0.011197 | 0.001151 | 0.004235 | 0.003847 | 0.024387 | 0.001931 | 0.003043 | 0.002886 | 0.023015 |
Vacuole Periphery | 0.000138 | 0.000246 | 0.000643 | 0.002241 | 0.000098 | 0.000535 | 0.000102 | 0.007562 | 0.000118 | 0.000404 | 0.000592 | 0.007009 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | -4.48 | -11.19 | -7.59 | -6.80 | -6.38 | -5.34 | -9.15 | -9.54 | -8.73 | -1.62 | -6.39 | -13.81 | -11.06 | -9.74 | -7.68 |
Bud Neck | -2.52 | -6.18 | -5.96 | -5.40 | -3.94 | -4.14 | -2.40 | -15.55 | -13.49 | -7.71 | -4.59 | -7.54 | -16.11 | -14.52 | -11.03 |
Bud Site | 0.41 | -0.97 | -4.80 | -4.87 | -4.55 | -3.25 | -3.27 | -9.30 | -8.28 | -8.27 | -1.95 | -2.15 | -9.72 | -9.26 | -8.78 |
Cell Periphery | 3.57 | 5.61 | 1.17 | -1.50 | -3.08 | 5.18 | 5.45 | 3.69 | -2.94 | -3.57 | 6.15 | 7.08 | 3.43 | -2.80 | -4.10 |
Cytoplasm | 19.63 | 23.47 | 12.68 | 0.38 | -1.70 | 3.03 | 12.92 | 3.93 | 1.19 | -10.51 | 16.94 | 23.79 | 15.33 | 0.37 | -7.04 |
Cytoplasmic Foci | 5.98 | 11.35 | 3.62 | -0.03 | -2.30 | 2.34 | 6.79 | 3.59 | 1.87 | -5.82 | 6.43 | 13.15 | 8.07 | 2.14 | -6.30 |
Eisosomes | 4.79 | 8.23 | -6.13 | -7.19 | -7.79 | 3.81 | 3.65 | 3.63 | -13.01 | -1.04 | 3.87 | 3.91 | 3.43 | -14.99 | -16.71 |
Endoplasmic Reticulum | -11.32 | -17.66 | -9.91 | -6.81 | -6.27 | -2.97 | -10.04 | -13.52 | -9.05 | 2.94 | -8.83 | -16.57 | -17.89 | -10.88 | -7.05 |
Endosome | 0.77 | 4.84 | -2.72 | -2.77 | -2.96 | -2.73 | -0.40 | -5.86 | -4.86 | -5.47 | -2.03 | 4.88 | -6.41 | -5.95 | -7.13 |
Golgi | 0.06 | 4.37 | -3.64 | -3.60 | -4.18 | -1.66 | 2.63 | -3.62 | -2.33 | -4.37 | -1.63 | 4.95 | -4.23 | -3.41 | -5.00 |
Lipid Particles | 1.50 | 5.85 | -1.21 | -1.66 | -2.55 | 0.79 | 2.95 | 1.88 | 1.95 | -2.37 | 1.26 | 5.16 | 1.72 | 0.77 | -4.04 |
Mitochondria | -2.74 | -0.17 | -1.68 | -0.96 | -1.67 | -3.87 | 0.78 | -4.21 | -0.10 | -4.70 | -4.69 | 0.62 | -4.77 | -1.27 | -4.97 |
Mitotic Spindle | -2.73 | -1.76 | -5.29 | -5.14 | -5.23 | -2.79 | 1.07 | -14.86 | -14.09 | -15.08 | -3.90 | -0.70 | -15.90 | -15.24 | -15.79 |
None | 11.43 | 14.38 | 9.12 | -0.71 | -3.49 | 8.39 | 8.45 | 7.33 | -2.74 | -2.97 | 14.08 | 16.44 | 12.75 | -1.73 | -6.72 |
Nuclear Periphery | -7.18 | -3.37 | -7.44 | -5.01 | -6.85 | -3.57 | 1.08 | -7.61 | -0.97 | -8.45 | -5.09 | -0.05 | -9.94 | -3.21 | -10.13 |
Nuclear Periphery Foci | 1.79 | 1.47 | -3.37 | -3.50 | -3.50 | 0.63 | -0.93 | -4.75 | -4.88 | -2.21 | 1.68 | 1.08 | -5.67 | -6.02 | -5.89 |
Nucleolus | 4.09 | 8.93 | -3.07 | -3.99 | -4.86 | 0.15 | -0.33 | -9.00 | -8.96 | -5.28 | 3.45 | 10.01 | -8.31 | -9.99 | -12.54 |
Nucleus | -19.31 | -25.81 | 2.64 | 8.27 | 9.57 | -3.72 | -6.73 | 15.20 | 19.18 | 19.52 | -16.21 | -25.28 | 11.57 | 22.18 | 27.23 |
Peroxisomes | -0.95 | 5.56 | -2.28 | -2.08 | -2.80 | -1.95 | 1.72 | -2.98 | -0.70 | -4.48 | -2.12 | 6.30 | -3.80 | -1.96 | -6.61 |
Vacuole | 3.19 | -0.95 | -3.94 | -4.09 | -3.89 | -4.57 | -4.15 | -10.48 | -8.70 | -7.22 | -2.85 | -4.26 | -10.90 | -10.23 | -10.17 |
Vacuole Periphery | -2.20 | -0.90 | -1.93 | -1.63 | -0.54 | -3.61 | -1.92 | -3.48 | -2.93 | -3.43 | -4.09 | -0.94 | -3.56 | -3.16 | -2.65 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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