Standard name
Human Ortholog
Description S-phase checkpoint protein required for DNA replication; couples DNA helicase and polymerase; interacts with and stabilizes Pol2p at stalled replication forks during stress, where it forms a pausing complex with Tof1p and is phosphorylated by Mec1p; defines a novel S-phase checkpoint with Hog1p that coordinates DNA replication and transcription upon osmostress; protects uncapped telomeres; Dia2p-dependent degradation mediates checkpoint recovery; mammalian claspin homolog
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 – 0.06 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0.05 0.05 0 0 0 0 – 0 0 0.13 0.18 0.17 0.09 0.08 0.12 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0.07
Golgi 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0.09 0.21 0.42 0.16 0.54 0.51 0.46 0.71 – 0.71 0.74 0.08 0 0.19 0 0 0.05 0 0 0 0 0 0
Nucleus 0.94 0.9 0.9 0.83 0.86 0.76 0.78 0.81 0.75 – 0.71 0.66 0.84 0.79 0.72 0.83 0.83 0.82 0.83 0.84 0.81 0.82 0.66 0.43
Nuclear Periphery 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 – 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0.06
Peroxisomes 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.14
Vac/Vac Membrane 0 0.05 0 0 0 0 0 0 0 – 0 0 0 0 0 0.07 0.08 0.05 0.05 0.09 0.1 0.1 0.18 0.19
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 1 0 0 0 0 0 0 1
Bud 0 0 0 0 0 3 3 2 4 – 8 4 0 1 1 0 0 0 4 6 4 1 5 5
Bud Neck 0 0 1 0 1 0 0 0 0 – 0 0 0 0 0 0 0 0 0 1 0 0 1 0
Bud Site 0 0 2 1 4 4 3 17 6 – 15 9 0 0 2 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 3 16 10 13 17 10 15 19 12 – 10 5 29 56 55 24 15 24 0 4 2 3 5 3
Endoplasmic Reticulum 3 0 1 0 0 0 0 0 0 – 0 0 0 0 0 6 2 7 0 0 0 0 2 0
Endosome 0 1 1 0 2 0 0 0 0 – 0 0 1 2 0 8 5 0 1 3 1 1 2 22
Golgi 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 1 1 2 3 1 0 2
Mitochondria 1 36 56 100 58 133 201 218 200 – 184 213 18 6 59 11 5 11 1 4 1 1 3 10
Nucleus 97 371 245 199 309 186 307 381 211 – 182 190 195 246 228 225 148 169 106 359 231 171 164 133
Nuclear Periphery 0 0 0 1 4 2 9 7 1 – 3 2 1 2 1 0 1 0 0 0 0 0 0 0
Nucleolus 3 5 1 3 8 10 8 18 8 – 6 12 11 9 14 4 4 3 2 3 6 0 3 17
Peroxisomes 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 1 0 2 3 3 0 3 4 1 – 1 1 1 2 0 2 5 1 2 2 5 2 11 45
Vac/Vac Membrane 0 19 3 2 11 4 4 3 4 – 4 6 4 5 8 19 14 11 6 36 28 20 44 58
Unique Cell Count 103 414 271 240 359 246 392 471 283 258 286 231 310 315 270 179 205 128 430 287 208 249 313
Labelled Cell Count 108 448 322 322 417 352 553 669 447 413 442 260 329 368 299 200 227 128 430 287 208 249 313


Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.9 5.1 4.3 4.2 4.5 3.9 3.9 3.8 3.3 – 3.2 3.0 5.3 5.3 4.5 6.7 7.2 7.2 5.1 5.1 5.6
Std Deviation (1e-4) 0.7 1.0 0.8 1.0 1.3 0.9 1.3 1.0 0.7 – 0.6 0.7 1.3 1.3 1.1 1.5 1.7 1.9 1.0 1.5 1.5
Intensity Change (Log2) – – – -0.03 0.09 -0.14 -0.11 -0.18 -0.37 – -0.41 -0.5 0.32 0.32 0.09 0.64 0.75 0.75 0.27 0.27 0.39


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Cytoplasm 0.9 0.6 0.2 0.1 0.2 0.3 – 0.1 -1.4 3.7 5.4 5.3 2.5 2.1 3.4
Endoplasmic Reticulum 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 – 0 0 -4.1 -7.3 0 -5.9 -5.4 -4.8
Nucleus -2.5 -1.7 -4.5 -4.1 -3.4 -4.9 – -5.8 -6.8 -2.0 -3.7 -5.5 -2.4 -2.4 -2.6
Nuclear Periphery 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 – 0 0 3.2 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Vacuole 0 1.6 0 0 0 0 – 0 0 0 0 1.3 3.5 3.7 2.7

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 4.0547 2.9082 2.8522 2.9393 2.1249 2.645 5.1252 3.6242 3.7745 3.6283 3.3681 3.4137 3.9913 4.1686 4.2223 4.5004 3.8035 4.0604
Actin 0.1428 0 0.0208 0.0409 0.0053 0.0004 0.0601 0 0.0096 0.0259 0.0155 0.002 0.0272 0 0.0005 0.0001 0 0.0003
Bud 0.0015 0 0.0002 0.0004 0.0007 0.0012 0.0013 0.0001 0.0005 0.0013 0.0129 0.0006 0.0001 0 0.0001 0 0 0.0002
Bud Neck 0.0161 0.0002 0.0005 0.0005 0.001 0.0015 0.0156 0.0003 0.0051 0.0155 0.0167 0.0022 0.0006 0.0003 0.0002 0.0001 0.0007 0.001
Bud Periphery 0.0032 0 0.0003 0.0003 0.0013 0.0005 0.0033 0.0001 0.0007 0.0027 0.0388 0.0009 0.0004 0 0.0002 0 0 0.0007
Bud Site 0.0091 0.0002 0.0013 0.0021 0.0024 0.0004 0.0228 0.0002 0.008 0.0102 0.0272 0.0006 0.0004 0.0004 0.0004 0 0.0001 0.0002
Cell Periphery 0.0019 0 0.0001 0 0.0004 0.0001 0.0008 0 0.0004 0.0007 0.0011 0.0001 0.0002 0 0.0001 0 0 0
Cytoplasm 0.0264 0.0011 0.0026 0.004 0.0026 0.0027 0.0329 0.001 0.0018 0.0032 0.0015 0.0035 0.0215 0.0048 0.0004 0.0027 0.0006 0.0093
Cytoplasmic Foci 0.0282 0 0.0023 0.0206 0.0124 0.0077 0.0122 0.0001 0.0068 0.0051 0.0017 0.0009 0.0039 0.0001 0 0.0001 0 0.0004
Eisosomes 0.0031 0 0.0005 0.0002 0.0001 0 0.0007 0 0.0001 0.0003 0.0001 0 0.0001 0 0 0 0 0
Endoplasmic Reticulum 0.0155 0.0001 0.0045 0.0002 0.0007 0.0005 0.0099 0.0001 0.0014 0.0014 0.0014 0.0038 0.0032 0.0003 0.0003 0.0001 0.0001 0.0003
Endosome 0.0494 0.0001 0.0117 0.0035 0.0307 0.0034 0.0222 0.0001 0.0109 0.0384 0.0057 0.0084 0.0111 0.0007 0.0002 0.0001 0.0001 0.0005
Golgi 0.0237 0 0.0092 0.0059 0.0214 0.0007 0.0105 0 0.0064 0.0068 0.0032 0.002 0.0024 0 0 0 0 0
Lipid Particles 0.0247 0 0.0019 0.01 0.016 0.0023 0.0049 0 0.0063 0.0058 0.0011 0.0004 0.0121 0 0 0 0 0.0004
Mitochondria 0.0121 0.0001 0.0035 0.0015 0.0348 0.0011 0.0138 0.0002 0.0047 0.0237 0.0048 0.0055 0.0067 0.0001 0.0015 0.0001 0.0002 0.0021
None 0.0342 0.0001 0.0011 0.0065 0.0039 0.0031 0.0172 0.0001 0.0004 0.0005 0.0003 0.0009 0.008 0.0094 0.0002 0.0281 0.0001 0.0055
Nuclear Periphery 0.0394 0.0028 0.0081 0.0009 0.0047 0.0058 0.0304 0.0039 0.013 0.0043 0.0033 0.0132 0.0265 0.0055 0.005 0.0023 0.0022 0.0019
Nucleolus 0.0134 0.0102 0.0074 0.0066 0.019 0.0206 0.0129 0.0103 0.0115 0.0043 0.0106 0.0184 0.0142 0.0109 0.0087 0.0054 0.0186 0.0167
Nucleus 0.4977 0.9815 0.9051 0.8582 0.7722 0.9392 0.6896 0.9638 0.8879 0.7925 0.84 0.9296 0.8283 0.9491 0.9805 0.9264 0.9377 0.9565
Peroxisomes 0.0225 0 0.0006 0.0195 0.0095 0.0006 0.0042 0 0.0015 0.0178 0.0007 0.0004 0.0021 0 0 0 0 0.0002
Punctate Nuclear 0.0221 0.0029 0.0161 0.0181 0.05 0.0072 0.0195 0.018 0.0195 0.0028 0.0013 0.0036 0.0142 0.0137 0.0007 0.0341 0.0395 0.0032
Vacuole 0.0091 0.0004 0.0014 0.0002 0.0077 0.0009 0.0076 0.0014 0.0026 0.0296 0.0094 0.0016 0.0113 0.0038 0.0005 0.0001 0.0001 0.0006
Vacuole Periphery 0.0039 0.0001 0.0007 0.0002 0.0031 0.0003 0.0076 0.0003 0.0008 0.0072 0.0025 0.0011 0.0054 0.0005 0.0004 0.0001 0 0.0002

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 26.99 8.3921 11.7874 16.5219 27.3864 30.4935 10.9097 7.9769 12.9721 27.8428
Translational Efficiency 1.981 1.451 0.7761 1.1595 1.2746 1.537 0.9082 0.9811 1.02 1.0847

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✔
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1525 1435 1595 148 1436 1723 167 1275 2961 3158 1762 1423

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 590.86 791.96 979.03 957.30 668.14 767.66 992.86 862.08 628.34 778.70 980.34 871.98
Standard Deviation 73.61 113.24 130.49 117.09 129.62 92.35 101.33 125.60 111.49 103.08 128.08 128.08
Intensity Change Log 2 — 0.422611 0.728537 0.696155 — 0.200317 0.571440 0.367671 — 0.308916 0.647301 0.531169

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000066 0.000295 0.000380 0.001936 0.000078 0.000179 0.000875 0.001364 0.000072 0.000232 0.000427 0.001424
Bud Neck 0.003834 0.005525 0.009203 0.008176 0.002280 0.005073 0.003823 0.024963 0.003080 0.005278 0.008693 0.023217
Bud Site 0.001464 0.001301 0.002094 0.005542 0.000482 0.001888 0.001147 0.020345 0.000988 0.001621 0.002005 0.018805
Cell Periphery 0.000282 0.000149 0.000083 0.000331 0.000632 0.000115 0.000073 0.000396 0.000452 0.000131 0.000082 0.000389
Cytoplasm 0.146581* 0.031179 0.016682 0.049209 0.054570 0.040236 0.004078 0.054411 0.101958* 0.036120 0.015488 0.053870
Cytoplasmic Foci 0.017233 0.005664 0.000092 0.011307 0.007793 0.005023 0.000116 0.006581 0.012655 0.005314 0.000095 0.007072
Eisosomes 0.000036 0.000022 0.000013 0.000082 0.000882 0.000021 0.000042 0.000043 0.000446 0.000021 0.000015 0.000047
Endoplasmic Reticulum 0.000701 0.004037 0.004406 0.007020 0.002000 0.003133 0.009193 0.006888 0.001331 0.003544 0.004860 0.006902
Endosome 0.000809 0.000689 0.000207 0.016820 0.000642 0.001344 0.000377 0.009546 0.000728 0.001047 0.000223 0.010302
Golgi 0.000182 0.000211 0.000039 0.002065 0.000312 0.000667 0.000060 0.003043 0.000245 0.000459 0.000041 0.002941
Lipid Particles 0.002006 0.001323 0.000100 0.009665 0.003026 0.002256 0.000329 0.001827 0.002501 0.001832 0.000121 0.002642
Mitochondria 0.000879 0.002222 0.000854 0.011355 0.001104 0.004978 0.000727 0.008952 0.000988 0.003726 0.000842 0.009201
Mitotic Spindle 0.000252 0.002859 0.000758 0.033895 0.001504 0.005890 0.000266 0.064585 0.000859 0.004513 0.000711 0.061393
None 0.048433 0.009141 0.002338 0.014042 0.038362 0.005488 0.004671 0.013026 0.043549 0.007148 0.002559 0.013131
Nuclear Periphery 0.000558 0.001306 0.000661 0.001795 0.000989 0.002464 0.000594 0.002158 0.000767 0.001938 0.000654 0.002120
Nuclear Periphery Foci 0.000573 0.000428 0.000186 0.002043 0.000838 0.000552 0.000414 0.002983 0.000701 0.000496 0.000207 0.002886
Nucleolus 0.014495 0.009259 0.000756 0.009986 0.009250 0.008067 0.005062 0.008523 0.011951 0.008609 0.001164 0.008675
Nucleus 0.758018* 0.921525* 0.957668* 0.791323* 0.873320* 0.906435* 0.963954* 0.736641* 0.813936* 0.913292* 0.958264* 0.742328*
Peroxisomes 0.000794 0.001008 0.000054 0.009969 0.000687 0.001422 0.000249 0.001780 0.000742 0.001234 0.000072 0.002631
Vacuole 0.002666 0.001611 0.002785 0.011197 0.001151 0.004235 0.003847 0.024387 0.001931 0.003043 0.002886 0.023015
Vacuole Periphery 0.000138 0.000246 0.000643 0.002241 0.000098 0.000535 0.000102 0.007562 0.000118 0.000404 0.000592 0.007009

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -4.48 -11.19 -7.59 -6.80 -6.38 -5.34 -9.15 -9.54 -8.73 -1.62 -6.39 -13.81 -11.06 -9.74 -7.68
Bud Neck -2.52 -6.18 -5.96 -5.40 -3.94 -4.14 -2.40 -15.55 -13.49 -7.71 -4.59 -7.54 -16.11 -14.52 -11.03
Bud Site 0.41 -0.97 -4.80 -4.87 -4.55 -3.25 -3.27 -9.30 -8.28 -8.27 -1.95 -2.15 -9.72 -9.26 -8.78
Cell Periphery 3.57 5.61 1.17 -1.50 -3.08 5.18 5.45 3.69 -2.94 -3.57 6.15 7.08 3.43 -2.80 -4.10
Cytoplasm 19.63 23.47 12.68 0.38 -1.70 3.03 12.92 3.93 1.19 -10.51 16.94 23.79 15.33 0.37 -7.04
Cytoplasmic Foci 5.98 11.35 3.62 -0.03 -2.30 2.34 6.79 3.59 1.87 -5.82 6.43 13.15 8.07 2.14 -6.30
Eisosomes 4.79 8.23 -6.13 -7.19 -7.79 3.81 3.65 3.63 -13.01 -1.04 3.87 3.91 3.43 -14.99 -16.71
Endoplasmic Reticulum -11.32 -17.66 -9.91 -6.81 -6.27 -2.97 -10.04 -13.52 -9.05 2.94 -8.83 -16.57 -17.89 -10.88 -7.05
Endosome 0.77 4.84 -2.72 -2.77 -2.96 -2.73 -0.40 -5.86 -4.86 -5.47 -2.03 4.88 -6.41 -5.95 -7.13
Golgi 0.06 4.37 -3.64 -3.60 -4.18 -1.66 2.63 -3.62 -2.33 -4.37 -1.63 4.95 -4.23 -3.41 -5.00
Lipid Particles 1.50 5.85 -1.21 -1.66 -2.55 0.79 2.95 1.88 1.95 -2.37 1.26 5.16 1.72 0.77 -4.04
Mitochondria -2.74 -0.17 -1.68 -0.96 -1.67 -3.87 0.78 -4.21 -0.10 -4.70 -4.69 0.62 -4.77 -1.27 -4.97
Mitotic Spindle -2.73 -1.76 -5.29 -5.14 -5.23 -2.79 1.07 -14.86 -14.09 -15.08 -3.90 -0.70 -15.90 -15.24 -15.79
None 11.43 14.38 9.12 -0.71 -3.49 8.39 8.45 7.33 -2.74 -2.97 14.08 16.44 12.75 -1.73 -6.72
Nuclear Periphery -7.18 -3.37 -7.44 -5.01 -6.85 -3.57 1.08 -7.61 -0.97 -8.45 -5.09 -0.05 -9.94 -3.21 -10.13
Nuclear Periphery Foci 1.79 1.47 -3.37 -3.50 -3.50 0.63 -0.93 -4.75 -4.88 -2.21 1.68 1.08 -5.67 -6.02 -5.89
Nucleolus 4.09 8.93 -3.07 -3.99 -4.86 0.15 -0.33 -9.00 -8.96 -5.28 3.45 10.01 -8.31 -9.99 -12.54
Nucleus -19.31 -25.81 2.64 8.27 9.57 -3.72 -6.73 15.20 19.18 19.52 -16.21 -25.28 11.57 22.18 27.23
Peroxisomes -0.95 5.56 -2.28 -2.08 -2.80 -1.95 1.72 -2.98 -0.70 -4.48 -2.12 6.30 -3.80 -1.96 -6.61
Vacuole 3.19 -0.95 -3.94 -4.09 -3.89 -4.57 -4.15 -10.48 -8.70 -7.22 -2.85 -4.26 -10.90 -10.23 -10.17
Vacuole Periphery -2.20 -0.90 -1.93 -1.63 -0.54 -3.61 -1.92 -3.48 -2.93 -3.43 -4.09 -0.94 -3.56 -3.16 -2.65
Protein Complexes26S Proteasome complex37S mitochondrial small ribosomal subunit54S mitochondrial large ribosomal subunit60S cytosolic large ribosomal subunitAar2-U5 snRNPAnaphase-Promoting Complex variant 1Anaphase-Promoting Complex variant 2Anaphase-Promoting Complex variant 3Anaphase-Promoting Complex variant 4Arp2/3 complexATG1 kinase complexBLOC-1 complexCcr4-Not complexCentral kinetochore CTF19 complexCleavage and polyadenylation specificity factorcomplexCMG helicase complexCMG-Pol epsilon complexCOMPASS complexCOP9 signalosome COPI vesicle coat complexCoQ6 biosynthesis complexCore mediator complexCORVET complexCTF18-RFC complexCURI complex variant 1CURI complex variant 2CURI complex variant 3Cytoplasmic exosome complex, DIS3 variantDASH complexDNA-directed RNA Polymerase I complexDNA-directed RNA polymerase II complexDNA-directed RNA polymerase III complexDNA replication factor C complexEGO complexELG1-RFC complex Elongator holoenzyme complexEndoplasmic Reticulum Membrane ComplexEukaryotic translation initiation factor 2BcomplexEukaryotic translation initiation factor 3core complexExocystExomer complexFAR complexGeneral transcription factor complex TFIIDGeneral transcription factor complex TFIIHGET4-GET5 transmembrane domain recognition complexGID ubiquitin ligase complexGin4 complexGolgi transport complexGSE complexHMC complexHOPS complexINO80 chromatin remodeling complexLSM1-7 complexLsm1-7-Pat1 complexLSM2-7 complexLSM2-8 complexMCM complexMICOS complexMitochondrial electron transport complex IIIMitochondrial inner membrane pre-sequence translocasecomplexMitochondrial outer membrane translocase holocomplexMitochondrial proton-transporting ATP synthase complexMitochondrial pyruvate dehydrogenase complexNineTeen complexNOP8 60s ribosome pre-assembly complexNuA3 histone acetyltransferase complexNuA4 histone acetyltransferase complexNuclear condensin complexNuclear/nucleolar exosome complex, DIS3-RRP6 variantNuclear pore complexNucleolar ribonuclease MRP complexNucleolar ribonuclease P complexOligosaccharyl transferase complex variant 1Oligosaccharyl transferase complex variant 2PAF1 complexPAS complexPhosphatidylinositol 3-kinase complex IPolarisomePrefoldin complexPRP19-associated complexRad17 RFC-like complexRetromer complexRPD3L histone deacetylase complexRPD3S histone deacetylase complexRQC complexRSC complex variant RSC1RSC complex variant RSC2SAGA complexSCF-Met30 ubiquitin ligase complexSCF-Ufo1 ubiquitin ligase complexSEA complexSeptin complexSET3C histone deacetylase complexSignal recognition particleSLIK (SAGA-like) complexSmall ribosomal subunit processome, variant1Small ribosomal subunit processome, variant2Small ribosomal subunit processome, variant3SMC5-SMC6 SUMO ligase complexSm complexSpliceosomal commitment complexSPOTS complexSWI/SNF chromatin remodelling complexSwr1 chromatin remodelling complexTHO complexTranscription factor TFIIIC complexTranslocon complexTRAPPIII protein complexTRAPPII protein complexTRAPPI protein complexTREX complexU1 snRNPU2 snRNPU4 snRNPU4/U6 snRNPU4/U6.U5 tri-snRNP complexU5 snRNPU6 snRNP complexUTP-A complexUTP-B complexUTP-C complex variant 1UTP-C complex variant 2UTP-C complex variant 3Vacuolar proton translocating ATPase complex,Golgi variantVacuolar proton translocating ATPase complex,vacuole variantCellular Components••••••••••••••••actinbud neckcell peripherycytoplasmendosomeERGolgimitochondrionmixednuclear peripherynucleolusnucleusperoxisomespindle polevacuolar membranevacuole
Standard name
Human Ortholog
Description S-phase checkpoint protein required for DNA replication; couples DNA helicase and polymerase; interacts with and stabilizes Pol2p at stalled replication forks during stress, where it forms a pausing complex with Tof1p and is phosphorylated by Mec1p; defines a novel S-phase checkpoint with Hog1p that coordinates DNA replication and transcription upon osmostress; protects uncapped telomeres; Dia2p-dependent degradation mediates checkpoint recovery; mammalian claspin homolog
Localization
Cell Percentages nucleus (86%)
Cell Cycle Regulation No
Subcompartmental Group nucleus-3

Mrc1

Mrc1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Mrc1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available