Standard name
Human Ortholog
Description Citrate synthase, peroxisomal isozyme involved in glyoxylate cycle; catalyzes condensation of acetyl coenzyme A and oxaloacetate to form citrate; expression is controlled by Rtg1p and Rtg2p transcription factors; SCF-Ucc1 regulates level of Cit2p to maintain citrate homeostasis; oxaloacetate-dependent positive feedback loop inhibits Cit2p ubiquitination; CIT2 has a paralog, CIT1, that arose from the whole genome duplication
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.09 0.09 0.05
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0
Cytoplasm 0.95 0.98 0.97 0.96 0.94 0.9 0.88 0.9 0.76 0.79 0.69 0.78 0.97 0.99 0.99 0.95 0.98 0.96 0.77 0.83 0.72 0.73 0.77 0.74
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.08 0.05 0.06 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.05 0.07 0.09 0.06 0.09 0.15 0.26 0.16 0.32 0.41 0.47 0.4 0 0 0 0.05 0 0 0.09 0.07 0.15 0 0 0.06
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 1 1 0 0 0 0 4 3 1 5 5 8
Bud 3 0 2 1 2 4 4 4 7 7 8 6 10 6 0 0 0 0 3 1 1 22 28 22
Bud Neck 2 1 0 0 0 1 2 4 0 2 0 0 0 0 0 0 0 0 2 1 1 3 3 5
Bud Site 0 0 0 0 0 0 1 0 2 1 0 1 0 0 0 0 0 0 – – – – – –
Cell Periphery 4 0 0 0 4 2 1 2 0 1 1 3 7 4 10 3 9 0 0 0 0 0 1 3
Cytoplasm 250 198 116 153 100 181 220 258 161 222 139 236 420 569 620 250 162 101 223 174 82 185 247 340
Endoplasmic Reticulum 1 0 0 1 0 0 0 1 2 0 1 0 0 0 2 21 9 6 9 0 0 3 3 5
Endosome 0 0 0 1 1 2 0 2 0 0 0 0 0 1 1 9 1 1 4 1 1 0 0 5
Golgi 0 0 0 0 1 0 0 0 0 0 0 0 0 1 0 1 0 1 1 1 0 0 1 2
Mitochondria 13 14 11 10 10 31 65 46 68 115 95 120 1 0 1 14 4 2 25 15 17 9 8 26
Nucleus 0 1 0 0 0 1 0 2 1 2 4 0 1 1 0 1 1 0 0 1 0 0 0 0
Nuclear Periphery 0 0 0 1 0 0 0 1 3 2 9 0 0 0 0 0 0 0 0 0 0 0 1 1
Nucleolus 1 0 0 1 0 1 0 1 1 1 0 0 0 0 1 1 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1
SpindlePole 3 0 0 0 0 2 1 3 1 2 0 4 3 0 0 0 0 1 4 1 0 11 10 8
Vac/Vac Membrane 1 0 0 0 3 2 2 4 4 6 13 1 2 4 3 2 1 3 3 3 2 3 3 14
Unique Cell Count 262 202 119 159 106 201 250 287 212 282 202 301 431 577 627 264 166 105 289 210 115 253 324 460
Labelled Cell Count 278 214 129 168 121 227 296 328 250 361 270 371 445 587 638 302 187 115 289 210 115 253 324 460


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 3.5 3.5 3.4 4.5 6.1 3.9 3.0 3.5 3.5 2.7 4.3 2.9 8.6 7.1 6.5 5.3 6.1 6.1 4.8 5.4 5.7
Std Deviation (1e-4) 0.9 0.7 1.4 1.5 2.7 1.2 1.0 1.6 1.5 1.0 1.9 1.3 5.8 4.3 3.0 2.7 2.3 1.9 2.2 2.1 1.9
Intensity Change (Log2) – – – 0.43 0.86 0.21 -0.18 0.06 0.07 -0.32 0.36 -0.23 1.34 1.07 0.95 0.65 0.84 0.86 0.52 0.69 0.75


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – –
Bud Neck – – – – – – – – – – – – – – –
Bud Site – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – –
Endosome – – – – – – – – – – – – – – –
Golgi – – – – – – – – – – – – – – –
Mitochondria – – – – – – – – – – – – – – –
Nuclear Periphery – – – – – – – – – – – – – – –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – –
Peroxisomes – – – – – – – – – – – – – – –
SpindlePole – – – – – – – – – – – – – – –
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – – –
Cytoplasm – – – – – – – – – – – – – – –
Nucleus – – – – – – – – – – – – – – –
Vacuole – – – – – – – – – – – – – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 15.507 18.2794 16.2134 15.957 15.7733 17.384 10.6404 17.0838 12.6151 11.4659 12.1876 13.5054 13.6276 17.875 16.7837 16.5331 15.6913 18.0224
Actin 0.0189 0.0007 0.0027 0.0223 0.0053 0.005 0.0383 0.0017 0.0041 0.0087 0.0038 0.0072 0.0341 0.0043 0.0262 0.0019 0.0059 0.002
Bud 0.0012 0.0006 0.0002 0.0031 0.0007 0.0002 0.0006 0.0003 0.0004 0.0009 0.0001 0.0002 0.0006 0.0022 0.001 0.0001 0 0.0002
Bud Neck 0.0025 0.0002 0.0005 0.0014 0.0027 0.0015 0.0008 0.0004 0.0004 0.0003 0.0004 0.0025 0.002 0.0004 0.0011 0.0005 0.0004 0.0014
Bud Periphery 0.0023 0.0002 0.0001 0.004 0.0006 0.0001 0.0005 0.0001 0.0004 0.0004 0.0001 0.0003 0.0008 0.0006 0.0007 0 0 0.0001
Bud Site 0.0039 0.0012 0.0003 0.0018 0.0006 0.0001 0.0051 0.0039 0.0008 0.001 0.0002 0.0002 0.0031 0.009 0.0034 0.0003 0.0002 0.0001
Cell Periphery 0.0009 0.0004 0.0002 0.0008 0.0002 0.0001 0.0003 0.0003 0.0002 0.0001 0.0001 0.0001 0.0017 0.0006 0.0002 0 0 0.0002
Cytoplasm 0.9214 0.9917 0.9645 0.9052 0.9511 0.9711 0.6978 0.9813 0.947 0.9061 0.9001 0.9239 0.7586 0.9736 0.93 0.9433 0.9356 0.9818
Cytoplasmic Foci 0.0038 0.0007 0.0041 0.006 0.007 0.0063 0.1011 0.0009 0.0018 0.0061 0.0075 0.0031 0.0478 0.0012 0.0069 0.0179 0.0296 0.0009
Eisosomes 0.0004 0 0 0 0 0 0.0003 0 0.0001 0 0.0001 0 0.0002 0 0.0001 0 0 0
Endoplasmic Reticulum 0.0081 0.0014 0.0058 0.0062 0.0053 0.0031 0.0049 0.0019 0.0018 0.0042 0.0078 0.0016 0.0131 0.004 0.0019 0.0009 0.0025 0.0019
Endosome 0.0073 0.0002 0.0061 0.002 0.0108 0.0018 0.0299 0.0002 0.005 0.0343 0.0029 0.0061 0.0288 0.0005 0.0056 0.0025 0.0067 0.0008
Golgi 0.0028 0.0001 0.0012 0.0029 0.0058 0.0018 0.0187 0.0001 0.0058 0.0063 0.004 0.0114 0.0081 0.0001 0.0057 0.0012 0.0024 0.0008
Lipid Particles 0.0022 0 0.0026 0.003 0.0005 0.0012 0.0241 0.0001 0.0007 0.0041 0.0078 0.002 0.0194 0.0002 0.0046 0.0123 0.005 0.0001
Mitochondria 0.0069 0 0.0008 0.0018 0.0012 0.0005 0.0062 0 0.0279 0.0033 0.0435 0.0056 0.0024 0.0001 0.0029 0.0011 0.0002 0.0073
None 0.0032 0.0004 0.001 0.0009 0.001 0.0007 0.0316 0.0059 0.0008 0.0011 0.0069 0.0075 0.023 0.0009 0.0012 0.0013 0.0019 0.0004
Nuclear Periphery 0.0041 0.0005 0.0032 0.001 0.0008 0.0006 0.0027 0.0006 0.0004 0.0151 0.0017 0.0006 0.0067 0.0006 0.001 0.0006 0.0007 0.0005
Nucleolus 0.0003 0 0.0001 0 0.0001 0 0.0002 0 0 0.0001 0 0.0002 0.0008 0 0.0001 0.0001 0 0
Nucleus 0.0038 0.0011 0.0021 0.0018 0.0028 0.0016 0.0009 0.0011 0.0006 0.0019 0.0009 0.0229 0.0057 0.001 0.0009 0.001 0.0009 0.001
Peroxisomes 0.0016 0 0.0015 0.0334 0.0006 0.0033 0.0264 0 0.0011 0.0008 0.0098 0.0027 0.0247 0 0.0044 0.0103 0.0034 0
Punctate Nuclear 0.0012 0.0001 0.0005 0.0003 0.0002 0.0002 0.0079 0.0009 0.0001 0.0023 0.0013 0.0014 0.0138 0.0002 0.0013 0.0042 0.0043 0.0001
Vacuole 0.0025 0.0005 0.002 0.0017 0.0025 0.0006 0.0012 0.0002 0.0003 0.0012 0.0006 0.0003 0.0037 0.0005 0.0006 0.0002 0.0001 0.0002
Vacuole Periphery 0.0008 0 0.0006 0.0002 0.0004 0.0001 0.0007 0 0.0003 0.0015 0.0002 0.0002 0.0009 0 0.0002 0.0001 0 0.0001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 183.9007 108.4087 100.0256 174.0709 137.0615 270.316 255.6409 258.4455 290.3919 242.9636
Translational Efficiency 1.1513 0.9296 1.4278 1.1795 1.1735 1.4566 1.2365 1.1787 1.2524 1.1995

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1540 1270 857 1510 597 1665 80 1053 2137 2935 937 2563

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 603.89 697.82 981.27 1388.86 734.48 689.31 1115.68 2230.37 640.37 692.99 992.75 1734.59
Standard Deviation 71.94 108.63 167.99 635.60 92.84 108.93 331.30 787.54 97.83 108.88 191.29 815.00
Intensity Change Log 2 — 0.208569 0.700364 1.201543 — -0.091570 0.603128 1.602488 — 0.051626 0.647815 1.435206

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000077 0.000469 0.000241 0.002672 0.000083 0.000720 0.000978 0.000589 0.000079 0.000611 0.000304 0.001816
Bud Neck 0.005508 0.034087 0.001686 0.005766 0.005312 0.050960 0.003769 0.003942 0.005453 0.043659 0.001864 0.005017
Bud Site 0.004096 0.025772 0.006523 0.052822 0.004632 0.019277 0.011459 0.064122 0.004246 0.022088 0.006944 0.057464
Cell Periphery 0.000228 0.000171 0.000145 0.004569 0.000193 0.000275 0.000378 0.000877 0.000218 0.000230 0.000165 0.003052
Cytoplasm 0.574209* 0.428145* 0.603367* 0.723047* 0.630470* 0.382923* 0.364832* 0.881317* 0.589926* 0.402491* 0.583001* 0.788072*
Cytoplasmic Foci 0.102276 0.265489* 0.030692 0.025251 0.170795 0.279806* 0.052111 0.006040 0.121418 0.273611* 0.032521 0.017358
Eisosomes 0.000129 0.000151 0.000049 0.000037 0.000102 0.000228 0.000106 0.000006 0.000121 0.000195 0.000054 0.000025
Endoplasmic Reticulum 0.000396 0.000721 0.001624 0.000565 0.000657 0.000678 0.004441 0.000040 0.000469 0.000697 0.001864 0.000349
Endosome 0.003069 0.026800 0.004836 0.002916 0.005566 0.038452 0.010332 0.000418 0.003766 0.033410 0.005306 0.001890
Golgi 0.000843 0.008287 0.000143 0.003399 0.000982 0.010429 0.000250 0.000360 0.000882 0.009502 0.000152 0.002150
Lipid Particles 0.003746 0.006946 0.002118 0.023101 0.003318 0.010726 0.008995 0.000205 0.003626 0.009090 0.002705 0.013694
Mitochondria 0.001265 0.013703 0.000236 0.000643 0.000863 0.019397 0.000117 0.000308 0.001153 0.016933 0.000225 0.000505
Mitotic Spindle 0.001754 0.007589 0.002142 0.008877 0.000695 0.004101 0.004728 0.001445 0.001458 0.005610 0.002362 0.005824
None 0.018942 0.003551 0.006505 0.005234 0.005073 0.003275 0.001395 0.007322 0.015068 0.003394 0.006069 0.006092
Nuclear Periphery 0.000231 0.000282 0.000922 0.000203 0.000245 0.000326 0.001194 0.000019 0.000235 0.000307 0.000945 0.000127
Nuclear Periphery Foci 0.000338 0.000639 0.003333 0.006061 0.000526 0.000386 0.014403 0.000041 0.000390 0.000495 0.004278 0.003588
Nucleolus 0.001911 0.001793 0.000535 0.000204 0.000677 0.002235 0.000237 0.000066 0.001567 0.002043 0.000509 0.000147
Nucleus 0.262341* 0.082761 0.222342* 0.050589 0.142158* 0.071707 0.227193* 0.014319 0.228766* 0.076490 0.222756* 0.035687
Peroxisomes 0.001609 0.016101 0.000307 0.001032 0.001199 0.019083 0.000559 0.000658 0.001495 0.017793 0.000329 0.000878
Vacuole 0.016536 0.073655 0.111975 0.081587 0.025769 0.081850 0.292308* 0.017070 0.019115 0.078304 0.127371 0.055081
Vacuole Periphery 0.000496 0.002889 0.000282 0.001426 0.000685 0.003167 0.000216 0.000836 0.000549 0.003047 0.000276 0.001184

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -5.86 -4.21 -5.12 -4.17 -4.56 -1.84 -2.11 -2.07 0.05 0.78 -2.69 -4.63 -5.51 -2.99 -4.33
Bud Neck -11.41 4.50 -0.74 11.58 -9.34 -16.34 0.03 1.38 17.26 0.64 -19.75 4.92 0.33 20.59 -7.14
Bud Site -9.96 -1.54 -16.77 -7.20 -15.10 -8.81 -2.28 -14.44 -10.28 -8.27 -13.11 -2.10 -21.90 -12.91 -18.96
Cell Periphery 3.33 5.38 -9.38 -9.55 -9.62 -1.68 -1.57 -4.11 -3.46 -2.14 -0.31 3.29 -10.06 -9.96 -10.30
Cytoplasm 14.88 1.52 -11.39 -23.37 -10.07 21.41 10.13 -23.83 -56.43* -19.24 26.03 4.95 -24.44 -48.84 -21.05
Cytoplasmic Foci -22.99 20.43 21.34 36.57 -0.05 -12.87 12.60 25.78 47.41 4.26 -29.45 26.85 32.79 58.73 5.13
Eisosomes -1.82 8.28 9.59 12.92 2.93 -8.24 0.14 9.12 20.26 7.50 -7.22 8.67 13.16 23.01 9.25
Endoplasmic Reticulum -2.47 -10.14 -1.74 1.06 9.76 -0.15 -5.58 5.82 9.62 6.51 -2.44 -11.52 1.17 4.86 13.23
Endosome -14.05 -2.73 1.58 15.00 5.34 -15.59 -1.50 7.95 18.95 5.45 -21.16 -2.35 5.14 23.63 8.93
Golgi -8.40 3.42 -1.81 5.89 -3.07 -8.72 4.16 2.81 9.31 -0.30 -11.78 4.69 -1.09 10.16 -3.19
Lipid Particles -4.46 3.76 -9.31 -7.23 -10.41 -9.34 -1.83 10.61 13.58 2.52 -9.41 1.45 -7.84 -2.65 -8.23
Mitochondria -8.11 6.94 3.93 8.64 -1.59 -10.73 3.93 2.84 11.11 -1.92 -13.32 7.70 5.14 14.00 -1.79
Mitotic Spindle -2.91 -1.35 -3.76 0.04 -2.95 -2.85 -1.68 -1.90 1.89 1.37 -3.51 -2.23 -3.78 0.32 -1.49
None 9.18 7.75 9.09 -0.54 1.24 1.70 3.38 -1.17 -2.77 -4.16 9.38 7.36 7.73 -2.61 -0.33
Nuclear Periphery -1.64 -17.31 -3.35 -0.48 15.60 -2.01 -7.25 10.93 8.38 8.60 -2.82 -18.63 3.07 4.52 19.45
Nuclear Periphery Foci -2.92 -12.01 -13.69 -12.91 -3.78 1.40 -5.17 6.41 5.31 5.39 -1.44 -12.70 -12.57 -12.19 2.66
Nucleolus 0.24 3.68 4.32 6.73 4.75 -3.48 7.46 10.84 4.79 4.27 -1.09 3.91 4.88 7.01 6.12
Nucleus 28.90 4.55 33.70 4.30 18.76 10.75 -2.16 20.98 19.35 6.92 32.04 1.22 40.26 11.68 22.65
Peroxisomes -10.84 3.49 2.12 11.92 -2.73 -15.67 0.52 1.62 15.97 0.38 -18.29 4.08 2.51 19.95 -2.47
Vacuole -16.93 -20.05 -24.11 -9.25 4.17 -15.68 -11.51 1.67 17.02 11.67 -24.60 -21.90 -20.96 -1.01 12.21
Vacuole Periphery -7.82 1.44 -0.84 4.45 -1.58 -10.18 5.92 -0.44 8.46 -3.50 -11.81 2.38 -1.00 7.71 -2.44
Protein Complexes26S Proteasome complex37S mitochondrial small ribosomal subunit54S mitochondrial large ribosomal subunit60S cytosolic large ribosomal subunitAar2-U5 snRNPAnaphase-Promoting Complex variant 1Anaphase-Promoting Complex variant 2Anaphase-Promoting Complex variant 3Anaphase-Promoting Complex variant 4Arp2/3 complexATG1 kinase complexBLOC-1 complexCcr4-Not complexCentral kinetochore CTF19 complexCleavage and polyadenylation specificity factorcomplexCMG helicase complexCMG-Pol epsilon complexCOMPASS complexCOP9 signalosome COPI vesicle coat complexCoQ6 biosynthesis complexCore mediator complexCORVET complexCTF18-RFC complexCURI complex variant 1CURI complex variant 2CURI complex variant 3Cytoplasmic exosome complex, DIS3 variantDASH complexDNA-directed RNA Polymerase I complexDNA-directed RNA polymerase II complexDNA-directed RNA polymerase III complexDNA replication factor C complexEGO complexELG1-RFC complex Elongator holoenzyme complexEndoplasmic Reticulum Membrane ComplexEukaryotic translation initiation factor 2BcomplexEukaryotic translation initiation factor 3core complexExocystExomer complexFAR complexGeneral transcription factor complex TFIIDGeneral transcription factor complex TFIIHGET4-GET5 transmembrane domain recognition complexGID ubiquitin ligase complexGin4 complexGolgi transport complexGSE complexHMC complexHOPS complexINO80 chromatin remodeling complexLSM1-7 complexLsm1-7-Pat1 complexLSM2-7 complexLSM2-8 complexMCM complexMICOS complexMitochondrial electron transport complex IIIMitochondrial inner membrane pre-sequence translocasecomplexMitochondrial outer membrane translocase holocomplexMitochondrial proton-transporting ATP synthase complexMitochondrial pyruvate dehydrogenase complexNineTeen complexNOP8 60s ribosome pre-assembly complexNuA3 histone acetyltransferase complexNuA4 histone acetyltransferase complexNuclear condensin complexNuclear/nucleolar exosome complex, DIS3-RRP6 variantNuclear pore complexNucleolar ribonuclease MRP complexNucleolar ribonuclease P complexOligosaccharyl transferase complex variant 1Oligosaccharyl transferase complex variant 2PAF1 complexPAS complexPhosphatidylinositol 3-kinase complex IPolarisomePrefoldin complexPRP19-associated complexRad17 RFC-like complexRetromer complexRPD3L histone deacetylase complexRPD3S histone deacetylase complexRQC complexRSC complex variant RSC1RSC complex variant RSC2SAGA complexSCF-Met30 ubiquitin ligase complexSCF-Ufo1 ubiquitin ligase complexSEA complexSeptin complexSET3C histone deacetylase complexSignal recognition particleSLIK (SAGA-like) complexSmall ribosomal subunit processome, variant1Small ribosomal subunit processome, variant2Small ribosomal subunit processome, variant3SMC5-SMC6 SUMO ligase complexSm complexSpliceosomal commitment complexSPOTS complexSWI/SNF chromatin remodelling complexSwr1 chromatin remodelling complexTHO complexTranscription factor TFIIIC complexTranslocon complexTRAPPIII protein complexTRAPPII protein complexTRAPPI protein complexTREX complexU1 snRNPU2 snRNPU4 snRNPU4/U6 snRNPU4/U6.U5 tri-snRNP complexU5 snRNPU6 snRNP complexUTP-A complexUTP-B complexUTP-C complex variant 1UTP-C complex variant 2UTP-C complex variant 3Vacuolar proton translocating ATPase complex,Golgi variantVacuolar proton translocating ATPase complex,vacuole variantCellular Components••••••••••••••••actinbud neckcell peripherycytoplasmendosomeERGolgimitochondrionmixednuclear peripherynucleolusnucleusperoxisomespindle polevacuolar membranevacuole
Standard name
Human Ortholog
Description Citrate synthase, peroxisomal isozyme involved in glyoxylate cycle; catalyzes condensation of acetyl coenzyme A and oxaloacetate to form citrate; expression is controlled by Rtg1p and Rtg2p transcription factors; SCF-Ucc1 regulates level of Cit2p to maintain citrate homeostasis; oxaloacetate-dependent positive feedback loop inhibits Cit2p ubiquitination; CIT2 has a paralog, CIT1, that arose from the whole genome duplication
Localization
Cell Percentages cytoplasm (61%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

Cit2

Cit2


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Cit2-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available