Standard name | |
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Human Ortholog | |
Description | Citrate synthase, peroxisomal isozyme involved in glyoxylate cycle; catalyzes condensation of acetyl coenzyme A and oxaloacetate to form citrate; expression is controlled by Rtg1p and Rtg2p transcription factors; SCF-Ucc1 regulates level of Cit2p to maintain citrate homeostasis; oxaloacetate-dependent positive feedback loop inhibits Cit2p ubiquitination; CIT2 has a paralog, CIT1, that arose from the whole genome duplication |
ensLOC | DeepLoc | |||||||||||||||||||||||
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Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 4 | 3 | 1 | 5 | 5 | 8 |
Bud | 3 | 0 | 2 | 1 | 2 | 4 | 4 | 4 | 7 | 7 | 8 | 6 | 10 | 6 | 0 | 0 | 0 | 0 | 3 | 1 | 1 | 22 | 28 | 22 |
Bud Neck | 2 | 1 | 0 | 0 | 0 | 1 | 2 | 4 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 1 | 1 | 3 | 3 | 5 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 2 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 4 | 0 | 0 | 0 | 4 | 2 | 1 | 2 | 0 | 1 | 1 | 3 | 7 | 4 | 10 | 3 | 9 | 0 | 0 | 0 | 0 | 0 | 1 | 3 |
Cytoplasm | 250 | 198 | 116 | 153 | 100 | 181 | 220 | 258 | 161 | 222 | 139 | 236 | 420 | 569 | 620 | 250 | 162 | 101 | 223 | 174 | 82 | 185 | 247 | 340 |
Endoplasmic Reticulum | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 2 | 0 | 1 | 0 | 0 | 0 | 2 | 21 | 9 | 6 | 9 | 0 | 0 | 3 | 3 | 5 |
Endosome | 0 | 0 | 0 | 1 | 1 | 2 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 9 | 1 | 1 | 4 | 1 | 1 | 0 | 0 | 5 |
Golgi | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 1 | 1 | 1 | 0 | 0 | 1 | 2 |
Mitochondria | 13 | 14 | 11 | 10 | 10 | 31 | 65 | 46 | 68 | 115 | 95 | 120 | 1 | 0 | 1 | 14 | 4 | 2 | 25 | 15 | 17 | 9 | 8 | 26 |
Nucleus | 0 | 1 | 0 | 0 | 0 | 1 | 0 | 2 | 1 | 2 | 4 | 0 | 1 | 1 | 0 | 1 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 3 | 2 | 9 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 |
Nucleolus | 1 | 0 | 0 | 1 | 0 | 1 | 0 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 |
SpindlePole | 3 | 0 | 0 | 0 | 0 | 2 | 1 | 3 | 1 | 2 | 0 | 4 | 3 | 0 | 0 | 0 | 0 | 1 | 4 | 1 | 0 | 11 | 10 | 8 |
Vac/Vac Membrane | 1 | 0 | 0 | 0 | 3 | 2 | 2 | 4 | 4 | 6 | 13 | 1 | 2 | 4 | 3 | 2 | 1 | 3 | 3 | 3 | 2 | 3 | 3 | 14 |
Unique Cell Count | 262 | 202 | 119 | 159 | 106 | 201 | 250 | 287 | 212 | 282 | 202 | 301 | 431 | 577 | 627 | 264 | 166 | 105 | 289 | 210 | 115 | 253 | 324 | 460 |
Labelled Cell Count | 278 | 214 | 129 | 168 | 121 | 227 | 296 | 328 | 250 | 361 | 270 | 371 | 445 | 587 | 638 | 302 | 187 | 115 | 289 | 210 | 115 | 253 | 324 | 460 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 3.5 | 3.5 | 3.4 | 4.5 | 6.1 | 3.9 | 3.0 | 3.5 | 3.5 | 2.7 | 4.3 | 2.9 | 8.6 | 7.1 | 6.5 | 5.3 | 6.1 | 6.1 | 4.8 | 5.4 | 5.7 |
Std Deviation (1e-4) | 0.9 | 0.7 | 1.4 | 1.5 | 2.7 | 1.2 | 1.0 | 1.6 | 1.5 | 1.0 | 1.9 | 1.3 | 5.8 | 4.3 | 3.0 | 2.7 | 2.3 | 1.9 | 2.2 | 2.1 | 1.9 |
Intensity Change (Log2) | – | – | – | 0.43 | 0.86 | 0.21 | -0.18 | 0.06 | 0.07 | -0.32 | 0.36 | -0.23 | 1.34 | 1.07 | 0.95 | 0.65 | 0.84 | 0.86 | 0.52 | 0.69 | 0.75 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Actin | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud Neck | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud Site | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cell Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cyto | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endoplasmic Reticulum | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endosome | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Golgi | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Mitochondria | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nuclear Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nuc | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleolus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Peroxisomes | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
SpindlePole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Vac | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cortical Patches | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cytoplasm | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Vacuole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 15.507 | 18.2794 | 16.2134 | 15.957 | 15.7733 | 17.384 | 10.6404 | 17.0838 | 12.6151 | 11.4659 | 12.1876 | 13.5054 | 13.6276 | 17.875 | 16.7837 | 16.5331 | 15.6913 | 18.0224 |
Actin | 0.0189 | 0.0007 | 0.0027 | 0.0223 | 0.0053 | 0.005 | 0.0383 | 0.0017 | 0.0041 | 0.0087 | 0.0038 | 0.0072 | 0.0341 | 0.0043 | 0.0262 | 0.0019 | 0.0059 | 0.002 |
Bud | 0.0012 | 0.0006 | 0.0002 | 0.0031 | 0.0007 | 0.0002 | 0.0006 | 0.0003 | 0.0004 | 0.0009 | 0.0001 | 0.0002 | 0.0006 | 0.0022 | 0.001 | 0.0001 | 0 | 0.0002 |
Bud Neck | 0.0025 | 0.0002 | 0.0005 | 0.0014 | 0.0027 | 0.0015 | 0.0008 | 0.0004 | 0.0004 | 0.0003 | 0.0004 | 0.0025 | 0.002 | 0.0004 | 0.0011 | 0.0005 | 0.0004 | 0.0014 |
Bud Periphery | 0.0023 | 0.0002 | 0.0001 | 0.004 | 0.0006 | 0.0001 | 0.0005 | 0.0001 | 0.0004 | 0.0004 | 0.0001 | 0.0003 | 0.0008 | 0.0006 | 0.0007 | 0 | 0 | 0.0001 |
Bud Site | 0.0039 | 0.0012 | 0.0003 | 0.0018 | 0.0006 | 0.0001 | 0.0051 | 0.0039 | 0.0008 | 0.001 | 0.0002 | 0.0002 | 0.0031 | 0.009 | 0.0034 | 0.0003 | 0.0002 | 0.0001 |
Cell Periphery | 0.0009 | 0.0004 | 0.0002 | 0.0008 | 0.0002 | 0.0001 | 0.0003 | 0.0003 | 0.0002 | 0.0001 | 0.0001 | 0.0001 | 0.0017 | 0.0006 | 0.0002 | 0 | 0 | 0.0002 |
Cytoplasm | 0.9214 | 0.9917 | 0.9645 | 0.9052 | 0.9511 | 0.9711 | 0.6978 | 0.9813 | 0.947 | 0.9061 | 0.9001 | 0.9239 | 0.7586 | 0.9736 | 0.93 | 0.9433 | 0.9356 | 0.9818 |
Cytoplasmic Foci | 0.0038 | 0.0007 | 0.0041 | 0.006 | 0.007 | 0.0063 | 0.1011 | 0.0009 | 0.0018 | 0.0061 | 0.0075 | 0.0031 | 0.0478 | 0.0012 | 0.0069 | 0.0179 | 0.0296 | 0.0009 |
Eisosomes | 0.0004 | 0 | 0 | 0 | 0 | 0 | 0.0003 | 0 | 0.0001 | 0 | 0.0001 | 0 | 0.0002 | 0 | 0.0001 | 0 | 0 | 0 |
Endoplasmic Reticulum | 0.0081 | 0.0014 | 0.0058 | 0.0062 | 0.0053 | 0.0031 | 0.0049 | 0.0019 | 0.0018 | 0.0042 | 0.0078 | 0.0016 | 0.0131 | 0.004 | 0.0019 | 0.0009 | 0.0025 | 0.0019 |
Endosome | 0.0073 | 0.0002 | 0.0061 | 0.002 | 0.0108 | 0.0018 | 0.0299 | 0.0002 | 0.005 | 0.0343 | 0.0029 | 0.0061 | 0.0288 | 0.0005 | 0.0056 | 0.0025 | 0.0067 | 0.0008 |
Golgi | 0.0028 | 0.0001 | 0.0012 | 0.0029 | 0.0058 | 0.0018 | 0.0187 | 0.0001 | 0.0058 | 0.0063 | 0.004 | 0.0114 | 0.0081 | 0.0001 | 0.0057 | 0.0012 | 0.0024 | 0.0008 |
Lipid Particles | 0.0022 | 0 | 0.0026 | 0.003 | 0.0005 | 0.0012 | 0.0241 | 0.0001 | 0.0007 | 0.0041 | 0.0078 | 0.002 | 0.0194 | 0.0002 | 0.0046 | 0.0123 | 0.005 | 0.0001 |
Mitochondria | 0.0069 | 0 | 0.0008 | 0.0018 | 0.0012 | 0.0005 | 0.0062 | 0 | 0.0279 | 0.0033 | 0.0435 | 0.0056 | 0.0024 | 0.0001 | 0.0029 | 0.0011 | 0.0002 | 0.0073 |
None | 0.0032 | 0.0004 | 0.001 | 0.0009 | 0.001 | 0.0007 | 0.0316 | 0.0059 | 0.0008 | 0.0011 | 0.0069 | 0.0075 | 0.023 | 0.0009 | 0.0012 | 0.0013 | 0.0019 | 0.0004 |
Nuclear Periphery | 0.0041 | 0.0005 | 0.0032 | 0.001 | 0.0008 | 0.0006 | 0.0027 | 0.0006 | 0.0004 | 0.0151 | 0.0017 | 0.0006 | 0.0067 | 0.0006 | 0.001 | 0.0006 | 0.0007 | 0.0005 |
Nucleolus | 0.0003 | 0 | 0.0001 | 0 | 0.0001 | 0 | 0.0002 | 0 | 0 | 0.0001 | 0 | 0.0002 | 0.0008 | 0 | 0.0001 | 0.0001 | 0 | 0 |
Nucleus | 0.0038 | 0.0011 | 0.0021 | 0.0018 | 0.0028 | 0.0016 | 0.0009 | 0.0011 | 0.0006 | 0.0019 | 0.0009 | 0.0229 | 0.0057 | 0.001 | 0.0009 | 0.001 | 0.0009 | 0.001 |
Peroxisomes | 0.0016 | 0 | 0.0015 | 0.0334 | 0.0006 | 0.0033 | 0.0264 | 0 | 0.0011 | 0.0008 | 0.0098 | 0.0027 | 0.0247 | 0 | 0.0044 | 0.0103 | 0.0034 | 0 |
Punctate Nuclear | 0.0012 | 0.0001 | 0.0005 | 0.0003 | 0.0002 | 0.0002 | 0.0079 | 0.0009 | 0.0001 | 0.0023 | 0.0013 | 0.0014 | 0.0138 | 0.0002 | 0.0013 | 0.0042 | 0.0043 | 0.0001 |
Vacuole | 0.0025 | 0.0005 | 0.002 | 0.0017 | 0.0025 | 0.0006 | 0.0012 | 0.0002 | 0.0003 | 0.0012 | 0.0006 | 0.0003 | 0.0037 | 0.0005 | 0.0006 | 0.0002 | 0.0001 | 0.0002 |
Vacuole Periphery | 0.0008 | 0 | 0.0006 | 0.0002 | 0.0004 | 0.0001 | 0.0007 | 0 | 0.0003 | 0.0015 | 0.0002 | 0.0002 | 0.0009 | 0 | 0.0002 | 0.0001 | 0 | 0.0001 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 183.9007 | 108.4087 | 100.0256 | 174.0709 | 137.0615 | 270.316 | 255.6409 | 258.4455 | 290.3919 | 242.9636 |
Translational Efficiency | 1.1513 | 0.9296 | 1.4278 | 1.1795 | 1.1735 | 1.4566 | 1.2365 | 1.1787 | 1.2524 | 1.1995 |
Dataset | Hit |
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Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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