Standard name
Human Ortholog
Description Putative Rheb-related GTPase; involved in regulating canavanine resistance and arginine uptake; member of the Ras superfamily of G-proteins
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.99 0.98 0.98 0.99 0.97 0.9 0.83 0.82 – 0.74 0.75 0.72 1.0 0.99 0.99 0.97 0.98 0.97 0.92 0.96 0.97 0.82 0.89 0.92
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0.06 0.21 0.34 0.32 – 0.38 0.39 0.38 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0.1 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 – 0 0 0 0 1 0 0 0 0 0 0 1 1 0 0
Bud 1 1 0 0 1 4 5 3 – 4 7 6 2 0 0 1 1 0 1 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 1 0 1
Bud Site 0 0 0 0 0 0 0 0 – 0 0 1 0 0 0 0 0 0 – – – – – –
Cell Periphery 10 10 0 4 4 9 4 5 – 10 7 12 5 3 8 5 0 2 0 0 0 0 0 0
Cytoplasm 324 306 231 289 347 350 430 387 – 318 262 292 201 269 302 111 48 72 330 312 221 117 201 244
Endoplasmic Reticulum 3 2 2 1 1 1 3 0 – 6 1 3 0 1 1 2 0 2 1 0 0 0 7 2
Endosome 0 0 0 0 1 1 0 0 – 0 1 0 0 1 0 0 0 0 5 1 0 0 1 1
Golgi 1 0 0 0 0 0 0 0 – 0 0 0 0 0 1 0 0 0 2 0 0 0 0 2
Mitochondria 0 1 6 12 23 80 177 152 – 162 137 155 0 0 0 2 1 3 1 0 0 2 2 2
Nucleus 4 3 0 0 1 3 2 6 – 1 1 3 1 0 2 2 0 4 0 0 1 1 0 1
Nuclear Periphery 0 0 1 0 0 3 4 0 – 2 1 2 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 1 – 1 0 0 0 0 0 0 1 0 0 0 0 0 0 1
Peroxisomes 0 0 0 0 0 0 0 0 – 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 1 0 1 0 4 10 13 – 17 8 10 1 1 0 1 0 1 9 7 0 13 5 4
Unique Cell Count 327 312 235 293 359 387 516 473 430 350 404 202 272 306 115 49 74 357 324 228 143 226 266
Labelled Cell Count 343 324 240 307 378 455 635 567 522 425 484 210 276 314 124 51 84 357 324 228 143 226 266


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 8.0 8.6 6.0 6.2 5.8 5.3 4.8 5.1 – 4.7 4.9 5.0 8.5 8.5 8.4 7.7 7.8 7.8 8.7 9.7 10.4
Std Deviation (1e-4) 1.1 1.1 0.9 1.4 1.4 1.3 1.4 1.3 – 0.9 0.9 1.1 1.2 1.2 1.1 1.0 1.5 1.2 1.8 1.6 1.6
Intensity Change (Log2) – – – 0.03 -0.05 -0.18 -0.32 -0.22 – -0.36 -0.29 -0.26 0.51 0.49 0.47 0.36 0.37 0.37 0.53 0.69 0.78


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Cytoplasm 0.3 -1.2 -3.8 -5.9 -6.2 – -7.9 -7.6 -8.2 1.2 0.6 0.4 -1.0 -0.2 -0.5
Endoplasmic Reticulum 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Mitochondria 1.0 2.1 6.3 0 8.9 – 0 10.1 10.1 0 0 0 0 0 0
Nucleus 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 – 3.1 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 2.6181 3.056 2.6451 2.1614 2.782 2.6462 3.5621 4.0211 3.7442 3.5186 4.0361 4.1119 2.3328 2.7173 2.4444 2.4436 2.0388 2.3965
Actin 0.011 0.0013 0.0093 0.0239 0.0329 0.005 0.0438 0.0016 0.0175 0.0003 0.045 0.0058 0.0004 0.0001 0.0003 0.0003 0.0002 0.0002
Bud 0.0036 0.0057 0.0008 0.0007 0.0003 0.0083 0.0003 0.0005 0.0009 0.0004 0.0038 0.0103 0 0.0001 0.0002 0.0003 0.0001 0
Bud Neck 0.0151 0.0002 0.0007 0.0006 0.0024 0.0021 0.0008 0.0003 0.0004 0.0002 0.0009 0.0023 0.0003 0.0001 0.0002 0.0003 0.0005 0.0007
Bud Periphery 0.0029 0.0016 0.0011 0.0009 0.0004 0.0053 0.0005 0.0008 0.0008 0.0005 0.0035 0.0091 0 0 0.0001 0.0004 0.0001 0
Bud Site 0.0022 0.0122 0.0015 0.0008 0.0004 0.0003 0.0006 0.0043 0.0066 0.0002 0.004 0.0011 0.0003 0.0003 0.0004 0.0002 0.0001 0
Cell Periphery 0.0004 0.0004 0.0003 0.0001 0.0001 0.0002 0.0003 0.0007 0.0005 0.0002 0.0002 0.0004 0.0001 0.0001 0.0001 0.0001 0 0
Cytoplasm 0.6811 0.8119 0.7356 0.7567 0.589 0.7754 0.7618 0.7666 0.8059 0.8872 0.6731 0.7956 0.8617 0.8938 0.9005 0.8683 0.9032 0.9039
Cytoplasmic Foci 0.0067 0.0053 0.009 0.0232 0.0059 0.0135 0.0043 0.0079 0.0063 0.01 0.0124 0.0121 0.0043 0.0033 0.0047 0.0111 0.0057 0.0032
Eisosomes 0.0002 0 0.0002 0.0001 0.0002 0 0.0003 0.0002 0.0001 0 0.0001 0 0 0 0 0 0 0
Endoplasmic Reticulum 0.0039 0.0023 0.0031 0.0071 0.002 0.0015 0.0048 0.0041 0.0035 0.0026 0.0093 0.002 0.0024 0.0011 0.0022 0.0026 0.0029 0.0012
Endosome 0.009 0.0021 0.0045 0.0115 0.0039 0.0013 0.0038 0.0031 0.0028 0.0038 0.0087 0.0027 0.0015 0.0012 0.0022 0.0085 0.0018 0.001
Golgi 0.0015 0.0002 0.0009 0.0055 0.003 0.0004 0.0041 0.0006 0.001 0.0003 0.0034 0.0005 0.0001 0.0001 0.0002 0.0003 0.0002 0.0001
Lipid Particles 0.0005 0.0001 0.0007 0.0045 0.0021 0.0009 0.001 0.0003 0.0003 0.0001 0.0006 0.0002 0.0001 0 0.0001 0.0001 0.0001 0
Mitochondria 0.0009 0.001 0.0006 0.0012 0.0005 0.0004 0.001 0.0003 0.0002 0.0001 0.0029 0.0003 0.0001 0.0001 0.0002 0.0002 0.0002 0.0001
None 0.2525 0.1495 0.2254 0.1478 0.3515 0.1779 0.1673 0.2018 0.1499 0.0898 0.2238 0.1526 0.1239 0.0971 0.0842 0.1028 0.0813 0.0877
Nuclear Periphery 0.0027 0.0008 0.0012 0.002 0.0012 0.0005 0.0014 0.0006 0.0007 0.0005 0.0021 0.0004 0.0013 0.0004 0.0006 0.0008 0.0006 0.0002
Nucleolus 0.0003 0.0001 0.0002 0.0001 0.0001 0.0001 0.0001 0.0002 0.0001 0 0.0002 0.0002 0 0 0 0.0001 0.0001 0
Nucleus 0.0021 0.0022 0.0016 0.0015 0.0008 0.0014 0.001 0.0016 0.001 0.001 0.0016 0.0015 0.0025 0.0014 0.0025 0.0016 0.0017 0.0009
Peroxisomes 0.0005 0.0001 0.0007 0.0082 0.0006 0.004 0.001 0.0001 0.0002 0.0001 0.0017 0.0005 0 0 0.0001 0.0001 0.0001 0
Punctate Nuclear 0.0013 0.0014 0.0012 0.0019 0.0018 0.0009 0.0009 0.0008 0.0006 0.0002 0.0015 0.0013 0.0003 0.0005 0.0005 0.0005 0.0006 0.0002
Vacuole 0.0013 0.0012 0.0014 0.0014 0.0007 0.0005 0.0005 0.0035 0.0008 0.0021 0.0012 0.0012 0.0006 0.0004 0.0007 0.0013 0.0006 0.0003
Vacuole Periphery 0.0002 0.0001 0.0003 0.0003 0.0002 0.0001 0.0002 0.0001 0.0001 0.0002 0.0003 0.0001 0.0001 0 0.0001 0.0002 0.0001 0

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 13.1358 14.0911 13.9416 12.8241 10.2187 7.0019 13.0602 10.6845 14.5008 13.2223
Translational Efficiency 0.6414 0.7845 0.8031 1.2316 1.3283 1.4552 1.0548 1.2647 0.823 1.0007

Hit Data

Dataset Hit
Protein Concentration ✔
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1203 1260 291 1663 1641 1636 2603 44 2844 2896 2894 1707

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 683.02 847.31 1081.67 938.00 827.76 884.62 1001.25 1030.51 766.54 868.39 1009.34 940.38
Standard Deviation 101.83 183.69 131.89 149.05 115.45 129.31 128.01 142.21 131.11 156.42 130.66 149.60
Intensity Change Log 2 — 0.310962 0.663261 0.457660 — 0.095845 0.274518 0.316074 — 0.197087 0.463314 0.381810

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000435 0.000277 0.001414 0.005419 0.000169 0.000508 0.000603 0.000335 0.000281 0.000407 0.000684 0.005288
Bud Neck 0.011036 0.006023 0.004127 0.012601 0.004139 0.011240 0.004591 0.010566 0.007057 0.008970 0.004545 0.012548
Bud Site 0.021018 0.010122 0.019127 0.055056 0.006081 0.020343 0.015369 0.007575 0.012399 0.015896 0.015747 0.053833
Cell Periphery 0.000373 0.000196 0.000313 0.000153 0.000077 0.000097 0.000071 0.000104 0.000202 0.000140 0.000095 0.000151
Cytoplasm 0.610325* 0.653779* 0.427135* 0.538483* 0.653725* 0.648849* 0.757802* 0.520012* 0.635367* 0.650994* 0.724552* 0.538007*
Cytoplasmic Foci 0.212390* 0.115961 0.019502 0.018519 0.142817 0.145106 0.010270 0.093626 0.172246 0.132425 0.011198 0.020455
Eisosomes 0.000209 0.000098 0.000061 0.000045 0.000058 0.000069 0.000016 0.000066 0.000122 0.000081 0.000020 0.000046
Endoplasmic Reticulum 0.003331 0.001008 0.007665 0.001213 0.000637 0.000435 0.000499 0.001817 0.001777 0.000684 0.001219 0.001229
Endosome 0.007260 0.007411 0.004508 0.008505 0.009733 0.012492 0.001620 0.006124 0.008687 0.010281 0.001910 0.008444
Golgi 0.005749 0.002055 0.000243 0.004274 0.001508 0.003254 0.001623 0.000373 0.003302 0.002732 0.001484 0.004173
Lipid Particles 0.003919 0.002112 0.002335 0.002303 0.001767 0.001328 0.000351 0.002154 0.002677 0.001669 0.000551 0.002299
Mitochondria 0.005584 0.001491 0.000492 0.003769 0.000581 0.001663 0.001040 0.000296 0.002697 0.001588 0.000985 0.003679
Mitotic Spindle 0.000510 0.003348 0.009599 0.084778 0.000731 0.004021 0.019004 0.021759 0.000637 0.003728 0.018058 0.083153
None 0.004214 0.018178 0.004281 0.009823 0.001399 0.000687 0.004372 0.004520 0.002589 0.008297 0.004363 0.009686
Nuclear Periphery 0.000173 0.000205 0.001563 0.000969 0.000172 0.000103 0.000481 0.000684 0.000173 0.000147 0.000590 0.000962
Nuclear Periphery Foci 0.000496 0.001389 0.006647 0.001384 0.000701 0.000684 0.000546 0.001808 0.000615 0.000990 0.001159 0.001395
Nucleolus 0.000838 0.001210 0.000254 0.001263 0.000315 0.000321 0.000143 0.001982 0.000536 0.000708 0.000154 0.001281
Nucleus 0.069603 0.083338 0.323952* 0.107356 0.057300 0.031582 0.108613 0.107723 0.062504 0.054101 0.130266* 0.107365
Peroxisomes 0.002418 0.002206 0.001799 0.004178 0.001301 0.004367 0.000192 0.001955 0.001773 0.003427 0.000354 0.004121
Vacuole 0.038083 0.088824 0.164776 0.134821 0.116188 0.111771 0.072365 0.216102* 0.083150 0.101787 0.081658 0.136916
Vacuole Periphery 0.002036 0.000767 0.000210 0.005089 0.000602 0.001079 0.000430 0.000419 0.001208 0.000943 0.000408 0.004968

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin 1.76 -3.80 -1.78 -2.01 -0.61 -3.61 -1.08 -4.06 0.20 -1.31 -1.91 -0.98 -2.01 -1.83 -1.83
Bud Neck 5.14 5.57 3.14 -1.47 -1.83 -6.93 -0.02 -1.00 0.32 -0.99 -2.43 5.06 -0.34 1.42 -3.46
Bud Site 5.96 2.44 -1.30 -6.21 -3.24 -9.37 -2.09 -1.51 3.84 -0.51 -2.74 2.98 -5.17 -3.75 -6.61
Cell Periphery 3.54 1.63 11.35 1.80 9.57 -3.93 2.48 -2.50 -1.23 -3.14 2.85 10.17 8.16 1.49 -1.84
Cytoplasm -3.35 18.11 15.69 18.64 -8.37 0.56 3.53 8.51 8.38 7.85 -1.71 6.08 21.76 22.80 16.13
Cytoplasmic Foci 14.69 36.14 38.03 25.51 1.60 -0.34 34.35 8.50 8.63 -2.64 9.51 49.97 48.79 40.82 -0.46
Eisosomes 3.15 6.77 8.37 1.78 5.51 -2.46 11.71 -3.32 -2.41 -6.47 2.64 10.78 8.66 2.73 -5.25
Endoplasmic Reticulum 3.28 -5.06 3.04 -0.99 9.00 2.82 -2.37 -4.47 -4.83 -4.18 3.58 0.60 1.54 -4.15 1.96
Endosome 0.03 4.52 6.16 5.31 2.10 -2.30 11.97 5.27 6.94 -3.86 -1.94 15.10 8.84 9.82 -4.30
Golgi 6.78 12.82 8.11 1.48 -2.91 -2.36 2.62 4.99 4.47 1.26 1.43 8.40 4.99 2.69 -1.71
Lipid Particles 4.15 3.34 5.88 1.49 3.48 1.80 8.16 -1.42 -2.38 -4.53 4.33 12.19 3.56 0.61 -1.65
Mitochondria 4.98 6.65 5.00 -0.34 -3.40 -2.71 -0.41 1.75 3.41 2.15 2.79 6.05 2.68 -0.06 -3.10
Mitotic Spindle -2.58 -2.67 -11.09 -9.96 -6.30 -2.90 -9.00 -1.88 -1.68 -0.92 -3.87 -9.44 -11.20 -10.17 -6.60
None -4.51 0.91 -2.21 3.64 -3.75 2.45 -9.61 -0.76 -1.73 3.33 -4.16 -4.25 -4.79 0.78 -2.88
Nuclear Periphery -1.13 -14.92 -17.77 -17.52 4.06 2.02 -22.96 -6.56 -6.91 -1.62 0.79 -27.90 -18.70 -19.12 -4.50
Nuclear Periphery Foci -6.26 -9.62 -4.04 1.63 8.47 0.15 -0.63 -3.32 -3.31 -3.27 -4.26 -8.10 -3.79 -0.91 1.03
Nucleolus -0.81 1.53 0.17 1.59 -2.97 -0.09 3.30 -0.92 -0.90 -1.02 -0.85 1.95 -1.04 -0.41 -3.33
Nucleus -3.04 -18.59 -17.22 -13.93 12.73 10.30 -27.77 -2.72 -3.87 2.06 2.97 -30.86 -21.88 -23.78 4.60
Peroxisomes 0.08 2.87 2.54 1.79 -0.70 -6.22 8.07 0.10 3.43 -1.39 -4.95 12.74 1.15 4.25 -2.73
Vacuole -13.78 -13.91 -33.61 -22.68 -2.12 0.57 -7.66 -7.45 -7.52 -6.56 -6.57 -18.39 -27.11 -23.61 -14.02
Vacuole Periphery 4.70 8.13 1.14 -1.84 -3.33 -2.11 3.64 4.73 3.99 -0.04 1.74 7.66 -0.71 -1.42 -3.29
Standard name
Human Ortholog
Description Putative Rheb-related GTPase; involved in regulating canavanine resistance and arginine uptake; member of the Ras superfamily of G-proteins
Localization
Cell Percentages cytoplasm (94%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

Rhb1

Rhb1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Rhb1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available