Standard name
Human Ortholog
Description Putative ribokinase
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0
Bud 0 – 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 – 0 0 0 0 0 0 0
Bud Neck 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0
Bud Site 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 – – – – – –
Cell Periphery 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0
Cytoplasm 1.0 – 0.96 0.95 0.94 0.91 0.89 0.82 0.78 0.75 0.77 0.8 0.91 0.93 0.98 0.95 – 1.0 0.99 0.91 0.92 0.83 0.81 0.87
Endoplasmic Reticulum 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 – 0 0 0 0 0 0 0
Endosome 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0
Golgi 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0
Mitochondria 0 – 0 0 0 0 0.09 0.11 0.19 0.14 0.2 0.11 0 0 0 0 – 0 0 0 0 0 0 0
Nucleus 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0
Nuclear Periphery 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0
Nucleolus 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0
Peroxisomes 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0
SpindlePole 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0
Vac/Vac Membrane 0 – 0 0.06 0.06 0.07 0.08 0.09 0.07 0.12 0.05 0.12 0.06 0.06 0 0.05 – 0 0 0 0 0.08 0.11 0.06
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 1 0
Bud 0 – 0 1 0 1 1 1 2 4 8 4 0 0 0 0 – 0 0 0 0 0 0 1
Bud Neck 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0
Bud Site 0 – 0 0 0 0 0 0 0 1 0 0 0 0 0 0 – 0 – – – – – –
Cell Periphery 1 – 1 4 3 7 4 0 2 2 1 2 0 2 1 0 – 0 0 0 0 0 0 0
Cytoplasm 258 – 96 182 220 250 248 168 188 184 133 183 113 177 198 18 – 17 251 39 118 92 115 211
Endoplasmic Reticulum 0 – 2 0 2 1 0 2 3 5 0 5 1 1 2 1 – 0 0 0 1 0 1 0
Endosome 0 – 0 0 0 0 2 0 0 0 0 0 0 0 0 0 – 0 0 1 0 0 0 0
Golgi 0 – 0 0 0 0 0 0 0 0 0 0 0 1 0 0 – 0 0 0 0 0 0 0
Mitochondria 1 – 0 0 0 8 24 23 47 33 34 25 1 0 1 0 – 0 1 0 0 2 0 3
Nucleus 0 – 1 2 4 4 4 3 7 7 3 7 3 4 3 0 – 0 0 0 1 3 3 2
Nuclear Periphery 0 – 0 0 0 0 0 0 0 0 0 0 1 0 0 0 – 0 0 0 0 0 0 1
Nucleolus 0 – 1 0 0 0 1 1 1 0 1 0 0 0 0 0 – 0 0 0 0 0 0 0
Peroxisomes 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 1 0 0
SpindlePole 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0
Vac/Vac Membrane 0 – 0 11 14 20 21 19 18 30 8 27 7 11 3 1 – 0 0 1 3 9 15 15
Unique Cell Count 259 100 192 234 274 278 204 242 244 172 230 124 190 203 19 17 255 44 129 112 142 243
Labelled Cell Count 260 101 200 243 291 305 217 268 266 188 253 126 196 208 20 17 255 44 129 112 142 243


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 7.2 – 7.7 8.6 8.6 7.7 7.4 7.2 6.5 6.3 6.5 6.8 8.4 9.6 10.6 16.0 – 16.1 7.8 8.9 10.5
Std Deviation (1e-4) 0.9 – 0.9 2.6 1.6 1.5 1.6 1.4 1.4 1.1 1.7 1.3 1.6 1.8 1.9 4.0 – 4.7 1.2 1.9 2.2
Intensity Change (Log2) – – – 0.16 0.16 0.01 -0.05 -0.09 -0.25 -0.29 -0.25 -0.17 0.12 0.31 0.47 1.06 – 1.07 0.03 0.2 0.45

WT3RAP60RAP140RAP220RAP300RAP380RAP460RAP540RAP620RAP70005101520WT3HU80HU120HU16005101520WT3rpd3Δ_1rpd3Δ_305101520WT1AF100AF140AF18005101520
rapamycinhydroxy-urearpd3 knockoutalpha-factorMultiple Condition Time-Course Intensity PlotsGFP Intensity (1e-4)GFP Intensity (1e-4)GFP Intensity (1e-4)GFP Intensity (1e-4)

Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0
Cytoplasm -0.5 -0.7 -1.5 -2.0 -3.3 -4.1 -4.5 -4.1 -3.8 -1.4 -1.0 0.7 -0.3 – 0.8
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0
Mitochondria 0 0 0 3.0 3.5 4.7 3.9 4.8 3.4 0 0 0 0 – 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0
Vacuole 0 0 2.8 2.8 3.2 2.8 3.7 0 3.6 0 0 0 0 – 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration – – – – – – – – – – – – – – – – – –
Actin – – – – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – – – – –
Bud Neck – – – – – – – – – – – – – – – – – –
Bud Periphery – – – – – – – – – – – – – – – – – –
Bud Site – – – – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – – – – –
Cytoplasm – – – – – – – – – – – – – – – – – –
Cytoplasmic Foci – – – – – – – – – – – – – – – – – –
Eisosomes – – – – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – – – – –
Endosome – – – – – – – – – – – – – – – – – –
Golgi – – – – – – – – – – – – – – – – – –
Lipid Particles – – – – – – – – – – – – – – – – – –
Mitochondria – – – – – – – – – – – – – – – – – –
None – – – – – – – – – – – – – – – – – –
Nuclear Periphery – – – – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – – – – –
Nucleus – – – – – – – – – – – – – – – – – –
Peroxisomes – – – – – – – – – – – – – – – – – –
Punctate Nuclear – – – – – – – – – – – – – – – – – –
Vacuole – – – – – – – – – – – – – – – – – –
Vacuole Periphery – – – – – – – – – – – – – – – – – –

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 22.5746 45.2827 29.2189 29.0864 24.2719 28.6154 30.1088 30.5661 35.9432 28.1603
Translational Efficiency 2.1298 1.0392 1.2488 1.7061 1.373 1.7449 1.7254 1.369 1.3962 1.4418

Hit Data

Dataset Hit
Protein Concentration –
Protein Localization –
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1883 434 487 1456 2319 1 493 1106 4202 435 980 2562

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 942.20 1080.66 1266.89 1283.48 918.18 1033.51 1285.07 1212.04 928.94 1080.55 1276.04 1252.64
Standard Deviation 116.19 141.12 164.26 194.67 119.60 — 158.41 199.73 118.68 140.97 161.60 200.03
Intensity Change Log 2 — 0.197807 0.427186 0.445956 — 0.170703 0.484998 0.400588 — 0.184494 0.456008 0.423743

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000412 0.001057 0.001730 0.001260 0.000116 0.006323 0.000311 0.002587 0.000249 0.001069 0.001016 0.001833
Bud Neck 0.001795 0.003051 0.003974 0.002450 0.001725 0.002179 0.001779 0.006842 0.001757 0.003049 0.002870 0.004346
Bud Site 0.005210 0.012995 0.018359 0.015738 0.008991 0.012971 0.004499 0.046248 0.007297 0.012995 0.011387 0.028909
Cell Periphery 0.000065 0.000104 0.000161 0.000150 0.000090 0.000678 0.000121 0.000448 0.000079 0.000106 0.000141 0.000279
Cytoplasm 0.765748 0.744737 0.532172 0.611251 0.787583 0.179242 0.635337 0.520402 0.777798 0.743437 0.584070 0.572032
Cytoplasmic Foci 0.084401 0.100847 0.013808 0.023703 0.060948 0.042739 0.015416 0.037198 0.071458 0.100713 0.014617 0.029529
Eisosomes 0.000020 0.000024 0.000017 0.000018 0.000016 0.000150 0.000025 0.000029 0.000018 0.000025 0.000021 0.000023
Endoplasmic Reticulum 0.000770 0.001294 0.002040 0.002044 0.000891 0.014341 0.002093 0.003706 0.000837 0.001324 0.002067 0.002761
Endosome 0.003528 0.006160 0.001810 0.010987 0.001884 0.039129 0.002618 0.031185 0.002621 0.006236 0.002216 0.019707
Golgi 0.001121 0.002822 0.000223 0.011981 0.000651 0.005258 0.000129 0.021374 0.000861 0.002828 0.000176 0.016036
Lipid Particles 0.000993 0.001046 0.001015 0.001343 0.000644 0.026625 0.001118 0.001282 0.000801 0.001105 0.001067 0.001316
Mitochondria 0.001188 0.002695 0.000350 0.001731 0.000307 0.000381 0.000172 0.017032 0.000702 0.002689 0.000260 0.008336
Mitotic Spindle 0.005025 0.003260 0.007089 0.051170 0.001056 0.000626 0.002501 0.119295 0.002835 0.003254 0.004781 0.080579
None 0.004768 0.004062 0.007102 0.004559 0.007705 0.001816 0.006913 0.006042 0.006388 0.004057 0.007007 0.005199
Nuclear Periphery 0.000149 0.000124 0.000987 0.001044 0.000125 0.000604 0.000939 0.000607 0.000136 0.000125 0.000963 0.000855
Nuclear Periphery Foci 0.001864 0.004312 0.003689 0.004112 0.001218 0.464407 0.007805 0.004135 0.001508 0.005369 0.005760 0.004122
Nucleolus 0.000335 0.000274 0.000169 0.000119 0.000288 0.010072 0.000162 0.000743 0.000309 0.000296 0.000166 0.000388
Nucleus 0.077467 0.048345 0.336056 0.142256 0.091793 0.048914 0.176214 0.046263 0.085374 0.048346 0.255646 0.100816
Peroxisomes 0.000792 0.002042 0.001493 0.000713 0.000616 0.002036 0.000148 0.007811 0.000695 0.002042 0.000816 0.003777
Vacuole 0.043709 0.059728 0.067603 0.098452 0.032985 0.137322 0.141577 0.115174 0.037791 0.059906 0.104817 0.105671
Vacuole Periphery 0.000639 0.001022 0.000154 0.014921 0.000366 0.004186 0.000121 0.011596 0.000488 0.001029 0.000137 0.013486

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -1.14 -1.79 -0.97 0.74 1.45 NaN NaN NaN NaN NaN -1.65 -2.67 -3.09 0.76 0.78
Bud Neck -0.76 -5.08 -2.09 0.23 3.57 NaN NaN NaN NaN NaN -0.81 -5.50 -4.33 0.03 1.98
Bud Site -3.40 -2.34 -2.82 2.11 1.26 NaN NaN NaN NaN NaN -2.48 -0.87 -3.21 0.84 -1.53
Cell Periphery -3.37 -11.08 -7.69 -1.95 4.82 NaN NaN NaN NaN NaN -1.97 -8.94 -2.38 -1.02 1.98
Cytoplasm 2.55 26.09 29.13 14.97 -5.75 NaN NaN NaN NaN NaN 3.80 34.11 42.56 16.39 -3.43
Cytoplasmic Foci -2.47 23.32 24.91 13.68 0.47 NaN NaN NaN NaN NaN -4.50 30.52 31.30 13.42 -0.55
Eisosomes -2.21 -3.11 0.06 2.23 3.14 NaN NaN NaN NaN NaN -2.92 -6.21 -1.33 2.43 6.82
Endoplasmic Reticulum -2.40 -7.85 -1.70 1.44 6.79 NaN NaN NaN NaN NaN -2.11 -9.10 -0.78 1.33 6.95
Endosome -2.58 3.43 2.85 3.90 0.36 NaN NaN NaN NaN NaN -3.73 0.72 -2.45 2.15 -2.77
Golgi -1.59 3.83 -1.02 0.81 -2.67 NaN NaN NaN NaN NaN -1.92 5.65 -3.16 0.02 -4.48
Lipid Particles -0.25 -4.38 -5.11 -3.30 -0.73 NaN NaN NaN NaN NaN -1.37 -9.00 -8.41 -2.17 0.50
Mitochondria -0.67 1.26 1.29 1.11 0.28 NaN NaN NaN NaN NaN -0.96 1.37 -1.55 0.47 -2.44
Mitotic Spindle 1.07 -1.59 -4.58 -4.96 -2.32 NaN NaN NaN NaN NaN 0.03 -3.08 -10.36 -8.52 -7.78
None 0.98 -1.89 -0.62 -1.34 1.58 NaN NaN NaN NaN NaN 2.59 0.73 3.01 -1.21 2.36
Nuclear Periphery 0.58 -15.40 -13.19 -13.95 5.60 NaN NaN NaN NaN NaN -0.06 -23.08 -20.44 -20.41 7.62
Nuclear Periphery Foci -5.13 -6.79 -4.48 2.75 3.59 NaN NaN NaN NaN NaN -3.51 -12.53 -6.69 2.33 7.98
Nucleolus 0.98 1.98 3.33 1.23 3.88 NaN NaN NaN NaN NaN 0.40 2.88 0.78 0.14 -0.93
Nucleus 7.20 -25.57 -19.27 -22.05 16.73 NaN NaN NaN NaN NaN 10.05 -27.14 -13.97 -18.46 20.18
Peroxisomes -1.67 0.34 3.43 2.63 1.56 NaN NaN NaN NaN NaN -1.86 1.45 -0.22 1.74 -1.36
Vacuole -4.44 -10.76 -30.09 -20.34 -10.35 NaN NaN NaN NaN NaN -5.72 -19.89 -43.85 -26.01 -10.13
Vacuole Periphery -0.73 1.49 -1.70 -1.34 -2.37 NaN NaN NaN NaN NaN -1.41 2.33 -2.83 -1.81 -3.54
Protein Complexes26S Proteasome complex37S mitochondrial small ribosomal subunit54S mitochondrial large ribosomal subunit60S cytosolic large ribosomal subunitAar2-U5 snRNPAnaphase-Promoting Complex variant 1Anaphase-Promoting Complex variant 2Anaphase-Promoting Complex variant 3Anaphase-Promoting Complex variant 4Arp2/3 complexATG1 kinase complexBLOC-1 complexCcr4-Not complexCentral kinetochore CTF19 complexCleavage and polyadenylation specificity factorcomplexCMG helicase complexCMG-Pol epsilon complexCOMPASS complexCOP9 signalosome COPI vesicle coat complexCoQ6 biosynthesis complexCore mediator complexCORVET complexCTF18-RFC complexCURI complex variant 1CURI complex variant 2CURI complex variant 3Cytoplasmic exosome complex, DIS3 variantDASH complexDNA-directed RNA Polymerase I complexDNA-directed RNA polymerase II complexDNA-directed RNA polymerase III complexDNA replication factor C complexEGO complexELG1-RFC complex Elongator holoenzyme complexEndoplasmic Reticulum Membrane ComplexEukaryotic translation initiation factor 2BcomplexEukaryotic translation initiation factor 3core complexExocystExomer complexFAR complexGeneral transcription factor complex TFIIDGeneral transcription factor complex TFIIHGET4-GET5 transmembrane domain recognition complexGID ubiquitin ligase complexGin4 complexGolgi transport complexGSE complexHMC complexHOPS complexINO80 chromatin remodeling complexLSM1-7 complexLsm1-7-Pat1 complexLSM2-7 complexLSM2-8 complexMCM complexMICOS complexMitochondrial electron transport complex IIIMitochondrial inner membrane pre-sequence translocasecomplexMitochondrial outer membrane translocase holocomplexMitochondrial proton-transporting ATP synthase complexMitochondrial pyruvate dehydrogenase complexNineTeen complexNOP8 60s ribosome pre-assembly complexNuA3 histone acetyltransferase complexNuA4 histone acetyltransferase complexNuclear condensin complexNuclear/nucleolar exosome complex, DIS3-RRP6 variantNuclear pore complexNucleolar ribonuclease MRP complexNucleolar ribonuclease P complexOligosaccharyl transferase complex variant 1Oligosaccharyl transferase complex variant 2PAF1 complexPAS complexPhosphatidylinositol 3-kinase complex IPolarisomePrefoldin complexPRP19-associated complexRad17 RFC-like complexRetromer complexRPD3L histone deacetylase complexRPD3S histone deacetylase complexRQC complexRSC complex variant RSC1RSC complex variant RSC2SAGA complexSCF-Met30 ubiquitin ligase complexSCF-Ufo1 ubiquitin ligase complexSEA complexSeptin complexSET3C histone deacetylase complexSignal recognition particleSLIK (SAGA-like) complexSmall ribosomal subunit processome, variant1Small ribosomal subunit processome, variant2Small ribosomal subunit processome, variant3SMC5-SMC6 SUMO ligase complexSm complexSpliceosomal commitment complexSPOTS complexSWI/SNF chromatin remodelling complexSwr1 chromatin remodelling complexTHO complexTranscription factor TFIIIC complexTranslocon complexTRAPPIII protein complexTRAPPII protein complexTRAPPI protein complexTREX complexU1 snRNPU2 snRNPU4 snRNPU4/U6 snRNPU4/U6.U5 tri-snRNP complexU5 snRNPU6 snRNP complexUTP-A complexUTP-B complexUTP-C complex variant 1UTP-C complex variant 2UTP-C complex variant 3Vacuolar proton translocating ATPase complex,Golgi variantVacuolar proton translocating ATPase complex,vacuole variantCellular Components••••••••••••••••actinbud neckcell peripherycytoplasmendosomeERGolgimitochondrionmixednuclear peripherynucleolusnucleusperoxisomespindle polevacuolar membranevacuole
Standard name
Human Ortholog
Description Putative ribokinase
Localization
Cell Percentages cytoplasm (99%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

Rbk1

Rbk1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Rbk1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available