Standard name
Human Ortholog
Description Component of the SF3b subcomplex of the U2 snRNP; increases efficiency of first and second step pre-mRNA splicing; diploid mutants display a random budding pattern instead of the wild-type bipolar pattern; facilitates passage through G1/S Start, but is not required for G2/M transition or exit from mitosis
DOWNLOAD

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 – 0 0 0 0.05 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0.05 0.06 0.1 – 0.05 0.08 0 0.06 0 0.1 0.14 0.13 0.11 0.11 0.08 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0.08 – 0.14 0.16 0.13 0.13 0.19 0 0 0 0 0.07 0 0 0 0 0 0 0
Nucleus 0.97 0.96 0.91 0.85 0.88 0.77 – 0.64 0.73 0.77 0.72 0.74 0.87 0.77 0.67 0.72 0.71 0.75 0.96 0.95 0.87 0.82 0.64 0.48
Nuclear Periphery 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0.19 0.09 0.15 – 0.26 0.26 0.25 0.26 0.26 0.07 0.16 0.2 0.08 0.07 0.1 0 0 0 0.06 0.11 0.12
Peroxisomes 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0.11 0.09 0.06 0 0 0.06 0.07 0.18 0.29
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0
Bud 0 0 0 0 1 0 – 1 0 1 0 0 0 0 0 1 1 1 1 0 0 0 2 5
Bud Neck 0 0 0 0 0 0 – 1 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 – 3 1 6 5 3 1 1 3 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 – 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0
Cytoplasm 9 2 3 3 6 13 – 10 10 8 6 3 24 28 34 30 22 20 0 1 0 3 1 3
Endoplasmic Reticulum 0 0 0 0 0 0 – 0 0 0 0 0 0 1 0 3 2 6 0 0 0 0 1 2
Endosome 0 0 0 0 0 0 – 0 0 0 0 0 1 0 5 3 2 3 0 1 0 1 1 3
Golgi 0 1 0 0 0 0 – 0 0 0 0 0 0 0 0 0 1 1 0 1 0 0 3 2
Mitochondria 1 2 2 1 0 10 – 29 21 24 13 26 2 0 2 6 15 4 0 0 1 0 1 4
Nucleus 362 207 77 50 90 99 – 129 94 138 71 103 217 158 171 201 144 177 363 213 86 139 164 136
Nuclear Periphery 0 0 0 0 0 4 – 6 1 1 0 4 2 0 4 5 3 2 0 0 0 0 0 2
Nucleolus 2 4 2 11 9 19 – 52 34 44 26 36 17 33 51 22 14 24 2 0 1 10 28 33
Peroxisomes 0 0 0 0 0 0 – 1 0 0 0 0 0 0 7 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 – 0 1 0 0 0 1 2 8 1 2 1 0 0 0 0 0 2
Vac/Vac Membrane 4 3 1 0 1 2 – 4 1 1 0 1 2 6 7 31 18 15 8 5 5 12 44 81
Unique Cell Count 375 216 85 59 102 129 201 129 179 99 140 249 205 256 279 202 237 378 225 100 170 255 282
Labelled Cell Count 378 219 85 65 107 147 236 163 223 121 177 267 230 292 303 224 255 378 225 100 170 255 282


Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 13.0 15.2 8.9 11.4 10.2 7.9 – 7.4 6.5 6.9 7.0 6.6 14.0 13.0 12.5 14.8 12.9 13.1 12.2 13.2 12.9
Std Deviation (1e-4) 3.8 3.8 3.2 3.2 3.3 2.5 – 2.9 2.3 2.4 3.5 2.2 4.6 4.5 5.0 4.7 4.5 4.1 3.2 3.6 4.4
Intensity Change (Log2) – – – 0.36 0.2 -0.17 – -0.26 -0.44 -0.36 -0.33 -0.43 0.66 0.55 0.49 0.73 0.54 0.57 0.46 0.58 0.54


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 1.8 – 0 1.3 0 0 0 1.8 2.5 2.5 2.0 2.0 1.5
Endoplasmic Reticulum 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 – 3.0 3.2 2.8 2.7 3.6 0 0 0 0 1.7 0
Nucleus -1.1 -0.5 -2.6 – -4.5 -3.2 -2.6 -3.2 -3.1 -0.8 -2.7 -4.3 -3.5 -3.5 -3.1
Nuclear Periphery 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 3.4 1.9 3.0 – 4.6 4.6 4.4 4.5 4.5 0 3.3 3.9 1.8 0 2.3
Peroxisomes 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 – 0 0 0 0 0 0 0 0 2.8 2.4 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 8.5779 8.1816 8.1853 7.9005 6.7017 7.2143 8.3007 8.2667 7.1574 6.6542 7.6209 7.0647 11.5923 10.5824 10.2 10.768 8.6189 9.7887
Actin 0.0032 0 0.0019 0.0004 0.0057 0.0016 0.0339 0.0016 0.0305 0.0025 0.0003 0.0004 0.0109 0 0 0 0.0207 0.0056
Bud 0.0006 0 0.0004 0 0.0002 0.0001 0.0023 0.0003 0.0046 0.0002 0.0001 0.0003 0.0003 0 0 0 0.0001 0.0001
Bud Neck 0.0004 0.0001 0.0005 0.0001 0.0002 0.0009 0.003 0.0002 0.0032 0.0003 0.0006 0.0007 0.0002 0.0001 0.0001 0 0.0003 0.0009
Bud Periphery 0.001 0.0001 0.0015 0.0001 0.0008 0.0003 0.0034 0.0005 0.0016 0.0002 0.0003 0.0009 0.0008 0 0 0 0.0004 0.0002
Bud Site 0.0041 0 0.0003 0.0001 0.0002 0.0002 0.0083 0.0018 0.0178 0.0005 0.0002 0.0003 0.0004 0 0 0 0.0003 0.0001
Cell Periphery 0.0004 0.0001 0.0004 0.0001 0.0001 0.0002 0.0009 0.0005 0.0003 0.0001 0.0002 0.0008 0.0003 0 0 0 0.0003 0.0001
Cytoplasm 0.0013 0 0.0003 0 0.0001 0.0001 0.0312 0.0274 0.0042 0.0691 0.0001 0.0002 0.0032 0 0 0 0.0003 0.0001
Cytoplasmic Foci 0.0133 0 0.0026 0.0003 0.0002 0.0092 0.0205 0.0011 0.0083 0.0177 0.0038 0.0013 0.0021 0 0 0 0.0035 0.0002
Eisosomes 0 0 0 0 0.0004 0 0.0004 0.0001 0.0003 0.0001 0 0 0.0002 0 0 0 0.0001 0.0001
Endoplasmic Reticulum 0.0009 0 0.0002 0 0.0003 0 0.0027 0.0003 0.0006 0.0003 0.0001 0.0002 0.0011 0 0 0 0.001 0.0003
Endosome 0.0143 0 0.0023 0.0002 0.0022 0.0022 0.0153 0.0032 0.0123 0.0173 0.0451 0.0098 0.0037 0 0 0 0.0027 0.0013
Golgi 0.0064 0 0.0043 0.0003 0.0025 0.0089 0.0082 0.0041 0.0077 0.0196 0.0087 0.007 0.002 0 0 0 0.0028 0.0009
Lipid Particles 0.0122 0 0.001 0.0058 0.0008 0.006 0.017 0.0011 0.0049 0.0113 0.0187 0.0024 0.005 0 0 0 0.0156 0.0011
Mitochondria 0.0053 0.0006 0.0167 0.0903 0.0961 0.0004 0.0455 0.1194 0.1114 0.14 0.0319 0.0656 0.0033 0.0002 0.0001 0.0001 0.0034 0.0007
None 0.0009 0 0.0001 0 0.0002 0 0.0037 0.0002 0.0004 0.0002 0.0001 0.0001 0.0037 0 0 0 0.0004 0.0005
Nuclear Periphery 0.0102 0.0013 0.002 0.0041 0.0011 0.0004 0.0127 0.0043 0.001 0.0011 0.0006 0.0007 0.0053 0.0011 0.0012 0.0097 0.0177 0.0036
Nucleolus 0.0454 0.0673 0.0555 0.0995 0.0849 0.1411 0.0394 0.0557 0.0571 0.0416 0.231 0.1566 0.0458 0.0502 0.0587 0.0655 0.2122 0.1543
Nucleus 0.8451 0.9262 0.898 0.7958 0.802 0.8193 0.6822 0.7677 0.7215 0.673 0.6461 0.7467 0.894 0.9478 0.9395 0.9243 0.6879 0.8268
Peroxisomes 0.0231 0 0.0016 0.0008 0.0006 0.0078 0.0379 0.005 0.0083 0.0029 0.0092 0.0008 0.0053 0 0 0 0.0154 0.0002
Punctate Nuclear 0.0027 0.0001 0.0002 0.0003 0.0005 0.0006 0.0153 0.0004 0.0006 0.0011 0.0005 0.0002 0.0076 0.0001 0.0001 0.0001 0.0017 0.002
Vacuole 0.0047 0.0014 0.0048 0.0004 0.0003 0.0005 0.0092 0.0027 0.0022 0.0005 0.001 0.0032 0.0031 0.0002 0.0001 0 0.0046 0.0004
Vacuole Periphery 0.0042 0.0026 0.0053 0.0014 0.0007 0.0002 0.0068 0.0025 0.0013 0.0005 0.001 0.0017 0.0016 0.0002 0.0001 0.0001 0.0087 0.0004

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 19.7868 6.2429 26.2508 16.9401 15.0908 18.6126 16.8325 11.3608 15.9375 18.8116
Translational Efficiency 1.0997 3.7291 0.8553 1.3899 1.6267 1.343 1.2065 2.1871 1.7039 1.3362

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1145 1148 597 1618 2298 1705 1663 1524 3443 2853 2260 3142

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 834.47 1178.84 1371.52 1270.77 1144.92 1210.38 1332.01 1293.44 1041.68 1197.69 1342.45 1281.77
Standard Deviation 206.40 268.16 221.61 228.56 221.99 228.18 225.50 237.37 261.63 245.54 225.15 233.15
Intensity Change Log 2 — 0.498436 0.716844 0.606771 — 0.080213 0.218358 0.175966 — 0.271484 0.449788 0.373459

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000471 0.007209 0.000698 0.000981 0.000507 0.000620 0.000502 0.000612 0.000495 0.003271 0.000554 0.000802
Bud Neck 0.003516 0.006731 0.011260 0.029143 0.003072 0.006534 0.011501 0.023010 0.003220 0.006613 0.011437 0.026169
Bud Site 0.004070 0.001937 0.000648 0.003050 0.000239 0.001096 0.000508 0.001584 0.001513 0.001435 0.000545 0.002339
Cell Periphery 0.000644 0.000172 0.000036 0.000130 0.000150 0.000333 0.000066 0.000104 0.000314 0.000268 0.000058 0.000118
Cytoplasm 0.007794 0.001054 0.000081 0.001033 0.000169 0.001209 0.000068 0.001181 0.002705 0.001147 0.000072 0.001105
Cytoplasmic Foci 0.001542 0.000368 0.000017 0.000789 0.000058 0.000528 0.000010 0.000506 0.000552 0.000464 0.000012 0.000652
Eisosomes 0.000075 0.000538 0.000070 0.000038 0.000126 0.000170 0.000093 0.000042 0.000109 0.000318 0.000087 0.000040
Endoplasmic Reticulum 0.005999 0.003288 0.001770 0.002552 0.005862 0.005312 0.001689 0.002600 0.005908 0.004498 0.001711 0.002575
Endosome 0.000395 0.000260 0.000032 0.000999 0.000201 0.000564 0.000040 0.001240 0.000265 0.000442 0.000038 0.001116
Golgi 0.000078 0.000119 0.000038 0.001038 0.000110 0.000325 0.000021 0.001086 0.000100 0.000242 0.000026 0.001061
Lipid Particles 0.000093 0.000189 0.000024 0.000214 0.000071 0.000385 0.000014 0.000062 0.000079 0.000306 0.000016 0.000140
Mitochondria 0.000606 0.002463 0.001025 0.003392 0.000530 0.001944 0.001081 0.002446 0.000555 0.002153 0.001066 0.002933
Mitotic Spindle 0.000016 0.001224 0.000024 0.008430 0.000170 0.002742 0.000496 0.004272 0.000118 0.002131 0.000372 0.006413
None 0.036118 0.001769 0.000859 0.002242 0.000583 0.001749 0.000433 0.001021 0.012400 0.001758 0.000545 0.001650
Nuclear Periphery 0.000094 0.000186 0.000065 0.002086 0.000137 0.000296 0.000220 0.000933 0.000123 0.000252 0.000179 0.001527
Nuclear Periphery Foci 0.000884 0.000973 0.000009 0.001161 0.000093 0.000453 0.000062 0.000374 0.000356 0.000662 0.000048 0.000779
Nucleolus 0.039112 0.030989 0.012944 0.025586 0.026830 0.024584 0.016187 0.019756 0.030915 0.027161 0.015331 0.022758
Nucleus 0.895327* 0.936106* 0.968977* 0.911753* 0.959148* 0.946021* 0.965998* 0.934513* 0.937924* 0.942032* 0.966785* 0.922793*
Peroxisomes 0.000548 0.000980 0.000839 0.000342 0.000238 0.000882 0.000396 0.000340 0.000341 0.000921 0.000513 0.000341
Vacuole 0.002304 0.002073 0.000496 0.003213 0.001333 0.002744 0.000438 0.002604 0.001656 0.002474 0.000454 0.002918
Vacuole Periphery 0.000312 0.001372 0.000088 0.001827 0.000371 0.001509 0.000176 0.001713 0.000352 0.001454 0.000153 0.001772

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -3.09 -6.58 -5.43 2.82 -2.72 -0.72 -0.08 -1.14 -1.20 -5.12 -3.14 -0.79 -3.11 2.72 -5.17
Bud Neck -3.51 -8.70 -18.56 -15.85 -13.36 -6.17 -14.32 -17.00 -13.73 -10.15 -6.97 -16.31 -25.69 -21.18 -16.63
Bud Site 1.68 3.21 0.93 -1.05 -3.64 -4.18 -3.85 -5.14 -2.10 -4.30 0.04 2.66 -1.89 -2.15 -5.31
Cell Periphery 4.53 6.23 5.36 1.54 -3.74 -1.98 2.99 1.60 2.58 -1.76 0.67 6.79 5.30 2.89 -3.26
Cytoplasm 4.36 5.52 4.61 -0.25 -4.85 -2.38 0.90 -2.12 0.23 -2.34 2.37 5.54 2.72 0.04 -4.36
Cytoplasmic Foci 2.10 2.76 1.69 -0.38 -1.51 -1.78 1.15 -1.81 0.37 -2.08 0.49 2.88 0.47 0.01 -2.32
Eisosomes -1.49 0.45 3.63 1.62 9.84 -2.31 3.08 9.13 7.64 7.75 -1.67 2.71 9.89 2.23 9.85
Endoplasmic Reticulum 5.57 9.44 7.54 2.45 -4.99 1.26 12.74 9.54 8.64 -5.69 4.36 15.85 12.18 8.74 -7.84
Endosome 1.45 5.06 -1.78 -2.59 -4.11 -1.89 1.74 -2.27 -1.13 -2.76 -1.49 3.50 -2.99 -1.88 -4.30
Golgi -1.29 3.32 -2.32 -2.12 -2.44 -1.52 1.17 -1.76 -1.24 -1.97 -1.66 1.45 -2.71 -2.10 -3.02
Lipid Particles -1.38 3.38 -0.85 0.18 -1.64 -1.91 0.95 0.11 2.06 -2.71 -2.21 1.56 -0.70 1.74 -2.16
Mitochondria -3.45 -7.35 -12.23 -1.71 -10.34 -3.44 -6.76 -7.56 -1.49 -5.52 -4.87 -8.63 -13.76 -2.53 -10.83
Mitotic Spindle -2.26 -1.56 -5.34 -4.37 -5.33 -2.73 -1.21 -3.44 -1.40 -3.07 -3.36 -1.29 -6.28 -3.97 -5.87
None 8.36 8.61 8.26 -0.97 -3.43 -2.54 0.98 -2.61 1.32 -3.66 7.46 8.50 7.62 0.13 -5.86
Nuclear Periphery -2.67 1.88 -4.88 -4.62 -4.94 -2.03 -0.73 -3.13 -2.43 -2.57 -2.59 -0.70 -5.75 -5.12 -5.19
Nuclear Periphery Foci -0.23 3.50 0.24 0.39 -3.28 -2.27 0.45 -1.87 0.81 -2.07 -1.39 3.25 -1.29 0.47 -3.69
Nucleolus 2.60 10.70 3.20 0.55 -7.52 1.02 5.18 2.14 1.10 -2.43 2.11 9.71 2.79 0.71 -6.10
Nucleus -5.49 -12.19 -1.02 5.39 14.94 4.01 -2.02 8.24 3.98 9.28 -0.95 -10.29 6.68 7.18 16.47
Peroxisomes -1.28 -3.41 2.87 1.83 6.16 -2.35 -2.75 -1.56 1.87 0.78 -2.74 -3.59 -0.56 2.61 3.08
Vacuole 0.35 3.99 -1.60 -1.96 -4.95 -3.34 4.88 -2.22 0.29 -3.84 -2.57 6.21 -3.18 -1.07 -6.33
Vacuole Periphery -1.89 4.64 -2.77 0.02 -3.36 -2.62 3.42 -2.28 0.01 -2.67 -3.20 4.64 -3.48 0.05 -4.12
Standard name
Human Ortholog
Description Component of the SF3b subcomplex of the U2 snRNP; increases efficiency of first and second step pre-mRNA splicing; diploid mutants display a random budding pattern instead of the wild-type bipolar pattern; facilitates passage through G1/S Start, but is not required for G2/M transition or exit from mitosis
Localization
Cell Percentages nucleus (100%)
Cell Cycle Regulation No
Subcompartmental Group nucleus-2

Bud31

Bud31


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Bud31-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available