Standard name | |
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Human Ortholog | |
Description | Component of the SF3b subcomplex of the U2 snRNP; increases efficiency of first and second step pre-mRNA splicing; diploid mutants display a random budding pattern instead of the wild-type bipolar pattern; facilitates passage through G1/S Start, but is not required for G2/M transition or exit from mitosis |
ensLOC | DeepLoc | |||||||||||||||||||||||
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Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 1 | 0 | – | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 2 | 5 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | – | 1 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | – | 3 | 1 | 6 | 5 | 3 | 1 | 1 | 3 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 9 | 2 | 3 | 3 | 6 | 13 | – | 10 | 10 | 8 | 6 | 3 | 24 | 28 | 34 | 30 | 22 | 20 | 0 | 1 | 0 | 3 | 1 | 3 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 3 | 2 | 6 | 0 | 0 | 0 | 0 | 1 | 2 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 5 | 3 | 2 | 3 | 0 | 1 | 0 | 1 | 1 | 3 |
Golgi | 0 | 1 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 1 | 0 | 0 | 3 | 2 |
Mitochondria | 1 | 2 | 2 | 1 | 0 | 10 | – | 29 | 21 | 24 | 13 | 26 | 2 | 0 | 2 | 6 | 15 | 4 | 0 | 0 | 1 | 0 | 1 | 4 |
Nucleus | 362 | 207 | 77 | 50 | 90 | 99 | – | 129 | 94 | 138 | 71 | 103 | 217 | 158 | 171 | 201 | 144 | 177 | 363 | 213 | 86 | 139 | 164 | 136 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 4 | – | 6 | 1 | 1 | 0 | 4 | 2 | 0 | 4 | 5 | 3 | 2 | 0 | 0 | 0 | 0 | 0 | 2 |
Nucleolus | 2 | 4 | 2 | 11 | 9 | 19 | – | 52 | 34 | 44 | 26 | 36 | 17 | 33 | 51 | 22 | 14 | 24 | 2 | 0 | 1 | 10 | 28 | 33 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | – | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 7 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 1 | 0 | 0 | 0 | 1 | 2 | 8 | 1 | 2 | 1 | 0 | 0 | 0 | 0 | 0 | 2 |
Vac/Vac Membrane | 4 | 3 | 1 | 0 | 1 | 2 | – | 4 | 1 | 1 | 0 | 1 | 2 | 6 | 7 | 31 | 18 | 15 | 8 | 5 | 5 | 12 | 44 | 81 |
Unique Cell Count | 375 | 216 | 85 | 59 | 102 | 129 | 201 | 129 | 179 | 99 | 140 | 249 | 205 | 256 | 279 | 202 | 237 | 378 | 225 | 100 | 170 | 255 | 282 | |
Labelled Cell Count | 378 | 219 | 85 | 65 | 107 | 147 | 236 | 163 | 223 | 121 | 177 | 267 | 230 | 292 | 303 | 224 | 255 | 378 | 225 | 100 | 170 | 255 | 282 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 13.0 | 15.2 | 8.9 | 11.4 | 10.2 | 7.9 | – | 7.4 | 6.5 | 6.9 | 7.0 | 6.6 | 14.0 | 13.0 | 12.5 | 14.8 | 12.9 | 13.1 | 12.2 | 13.2 | 12.9 |
Std Deviation (1e-4) | 3.8 | 3.8 | 3.2 | 3.2 | 3.3 | 2.5 | – | 2.9 | 2.3 | 2.4 | 3.5 | 2.2 | 4.6 | 4.5 | 5.0 | 4.7 | 4.5 | 4.1 | 3.2 | 3.6 | 4.4 |
Intensity Change (Log2) | – | – | – | 0.36 | 0.2 | -0.17 | – | -0.26 | -0.44 | -0.36 | -0.33 | -0.43 | 0.66 | 0.55 | 0.49 | 0.73 | 0.54 | 0.57 | 0.46 | 0.58 | 0.54 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 0 | 0 | 1.8 | – | 0 | 1.3 | 0 | 0 | 0 | 1.8 | 2.5 | 2.5 | 2.0 | 2.0 | 1.5 |
Endoplasmic Reticulum | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 0 | – | 3.0 | 3.2 | 2.8 | 2.7 | 3.6 | 0 | 0 | 0 | 0 | 1.7 | 0 |
Nucleus | -1.1 | -0.5 | -2.6 | – | -4.5 | -3.2 | -2.6 | -3.2 | -3.1 | -0.8 | -2.7 | -4.3 | -3.5 | -3.5 | -3.1 |
Nuclear Periphery | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 3.4 | 1.9 | 3.0 | – | 4.6 | 4.6 | 4.4 | 4.5 | 4.5 | 0 | 3.3 | 3.9 | 1.8 | 0 | 2.3 |
Peroxisomes | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2.8 | 2.4 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 8.5779 | 8.1816 | 8.1853 | 7.9005 | 6.7017 | 7.2143 | 8.3007 | 8.2667 | 7.1574 | 6.6542 | 7.6209 | 7.0647 | 11.5923 | 10.5824 | 10.2 | 10.768 | 8.6189 | 9.7887 |
Actin | 0.0032 | 0 | 0.0019 | 0.0004 | 0.0057 | 0.0016 | 0.0339 | 0.0016 | 0.0305 | 0.0025 | 0.0003 | 0.0004 | 0.0109 | 0 | 0 | 0 | 0.0207 | 0.0056 |
Bud | 0.0006 | 0 | 0.0004 | 0 | 0.0002 | 0.0001 | 0.0023 | 0.0003 | 0.0046 | 0.0002 | 0.0001 | 0.0003 | 0.0003 | 0 | 0 | 0 | 0.0001 | 0.0001 |
Bud Neck | 0.0004 | 0.0001 | 0.0005 | 0.0001 | 0.0002 | 0.0009 | 0.003 | 0.0002 | 0.0032 | 0.0003 | 0.0006 | 0.0007 | 0.0002 | 0.0001 | 0.0001 | 0 | 0.0003 | 0.0009 |
Bud Periphery | 0.001 | 0.0001 | 0.0015 | 0.0001 | 0.0008 | 0.0003 | 0.0034 | 0.0005 | 0.0016 | 0.0002 | 0.0003 | 0.0009 | 0.0008 | 0 | 0 | 0 | 0.0004 | 0.0002 |
Bud Site | 0.0041 | 0 | 0.0003 | 0.0001 | 0.0002 | 0.0002 | 0.0083 | 0.0018 | 0.0178 | 0.0005 | 0.0002 | 0.0003 | 0.0004 | 0 | 0 | 0 | 0.0003 | 0.0001 |
Cell Periphery | 0.0004 | 0.0001 | 0.0004 | 0.0001 | 0.0001 | 0.0002 | 0.0009 | 0.0005 | 0.0003 | 0.0001 | 0.0002 | 0.0008 | 0.0003 | 0 | 0 | 0 | 0.0003 | 0.0001 |
Cytoplasm | 0.0013 | 0 | 0.0003 | 0 | 0.0001 | 0.0001 | 0.0312 | 0.0274 | 0.0042 | 0.0691 | 0.0001 | 0.0002 | 0.0032 | 0 | 0 | 0 | 0.0003 | 0.0001 |
Cytoplasmic Foci | 0.0133 | 0 | 0.0026 | 0.0003 | 0.0002 | 0.0092 | 0.0205 | 0.0011 | 0.0083 | 0.0177 | 0.0038 | 0.0013 | 0.0021 | 0 | 0 | 0 | 0.0035 | 0.0002 |
Eisosomes | 0 | 0 | 0 | 0 | 0.0004 | 0 | 0.0004 | 0.0001 | 0.0003 | 0.0001 | 0 | 0 | 0.0002 | 0 | 0 | 0 | 0.0001 | 0.0001 |
Endoplasmic Reticulum | 0.0009 | 0 | 0.0002 | 0 | 0.0003 | 0 | 0.0027 | 0.0003 | 0.0006 | 0.0003 | 0.0001 | 0.0002 | 0.0011 | 0 | 0 | 0 | 0.001 | 0.0003 |
Endosome | 0.0143 | 0 | 0.0023 | 0.0002 | 0.0022 | 0.0022 | 0.0153 | 0.0032 | 0.0123 | 0.0173 | 0.0451 | 0.0098 | 0.0037 | 0 | 0 | 0 | 0.0027 | 0.0013 |
Golgi | 0.0064 | 0 | 0.0043 | 0.0003 | 0.0025 | 0.0089 | 0.0082 | 0.0041 | 0.0077 | 0.0196 | 0.0087 | 0.007 | 0.002 | 0 | 0 | 0 | 0.0028 | 0.0009 |
Lipid Particles | 0.0122 | 0 | 0.001 | 0.0058 | 0.0008 | 0.006 | 0.017 | 0.0011 | 0.0049 | 0.0113 | 0.0187 | 0.0024 | 0.005 | 0 | 0 | 0 | 0.0156 | 0.0011 |
Mitochondria | 0.0053 | 0.0006 | 0.0167 | 0.0903 | 0.0961 | 0.0004 | 0.0455 | 0.1194 | 0.1114 | 0.14 | 0.0319 | 0.0656 | 0.0033 | 0.0002 | 0.0001 | 0.0001 | 0.0034 | 0.0007 |
None | 0.0009 | 0 | 0.0001 | 0 | 0.0002 | 0 | 0.0037 | 0.0002 | 0.0004 | 0.0002 | 0.0001 | 0.0001 | 0.0037 | 0 | 0 | 0 | 0.0004 | 0.0005 |
Nuclear Periphery | 0.0102 | 0.0013 | 0.002 | 0.0041 | 0.0011 | 0.0004 | 0.0127 | 0.0043 | 0.001 | 0.0011 | 0.0006 | 0.0007 | 0.0053 | 0.0011 | 0.0012 | 0.0097 | 0.0177 | 0.0036 |
Nucleolus | 0.0454 | 0.0673 | 0.0555 | 0.0995 | 0.0849 | 0.1411 | 0.0394 | 0.0557 | 0.0571 | 0.0416 | 0.231 | 0.1566 | 0.0458 | 0.0502 | 0.0587 | 0.0655 | 0.2122 | 0.1543 |
Nucleus | 0.8451 | 0.9262 | 0.898 | 0.7958 | 0.802 | 0.8193 | 0.6822 | 0.7677 | 0.7215 | 0.673 | 0.6461 | 0.7467 | 0.894 | 0.9478 | 0.9395 | 0.9243 | 0.6879 | 0.8268 |
Peroxisomes | 0.0231 | 0 | 0.0016 | 0.0008 | 0.0006 | 0.0078 | 0.0379 | 0.005 | 0.0083 | 0.0029 | 0.0092 | 0.0008 | 0.0053 | 0 | 0 | 0 | 0.0154 | 0.0002 |
Punctate Nuclear | 0.0027 | 0.0001 | 0.0002 | 0.0003 | 0.0005 | 0.0006 | 0.0153 | 0.0004 | 0.0006 | 0.0011 | 0.0005 | 0.0002 | 0.0076 | 0.0001 | 0.0001 | 0.0001 | 0.0017 | 0.002 |
Vacuole | 0.0047 | 0.0014 | 0.0048 | 0.0004 | 0.0003 | 0.0005 | 0.0092 | 0.0027 | 0.0022 | 0.0005 | 0.001 | 0.0032 | 0.0031 | 0.0002 | 0.0001 | 0 | 0.0046 | 0.0004 |
Vacuole Periphery | 0.0042 | 0.0026 | 0.0053 | 0.0014 | 0.0007 | 0.0002 | 0.0068 | 0.0025 | 0.0013 | 0.0005 | 0.001 | 0.0017 | 0.0016 | 0.0002 | 0.0001 | 0.0001 | 0.0087 | 0.0004 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 19.7868 | 6.2429 | 26.2508 | 16.9401 | 15.0908 | 18.6126 | 16.8325 | 11.3608 | 15.9375 | 18.8116 |
Translational Efficiency | 1.0997 | 3.7291 | 0.8553 | 1.3899 | 1.6267 | 1.343 | 1.2065 | 2.1871 | 1.7039 | 1.3362 |
Dataset | Hit |
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Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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