Standard name | |
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Human Ortholog | |
Description | Deadenylation-dependent mRNA-decapping factor; also required for faithful chromosome transmission, maintenance of rDNA locus stability, and protection of mRNA 3'-UTRs from trimming; associated with topoisomerase II; binds to mRNAs under glucose starvation, most often in the 3' UTR; functionally linked to Pab1p; forms cytoplasmic foci upon DNA replication stress; phosphorylation by PKA inhibits P body foci formation |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 2 | 2 | 3 | 5 | 9 | 15 | 13 | – | 8 | 15 | 6 | 24 | 19 | 0 | 4 | 3 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 2 | 4 | 3 | 6 | 11 | 9 | 10 | 6 | – | 6 | 3 | 2 | 12 | 21 | 0 | 0 | 1 | 0 | 2 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | – | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 2 | 3 | 10 | 10 | 11 | 11 | 8 | 6 | 5 | – | 9 | 10 | 2 | 4 | 4 | 24 | 21 | 42 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 143 | 198 | 209 | 196 | 233 | 165 | 230 | 203 | 112 | – | 105 | 107 | 82 | 120 | 114 | 299 | 301 | 259 | 143 | 218 | 213 | 112 | 146 | 167 |
Endoplasmic Reticulum | 4 | 15 | 2 | 0 | 10 | 7 | 14 | 4 | 11 | – | 31 | 19 | 19 | 14 | 19 | 15 | 5 | 4 | 10 | 7 | 8 | 33 | 40 | 42 |
Endosome | 0 | 0 | 2 | 2 | 2 | 6 | 8 | 16 | 2 | – | 1 | 2 | 1 | 24 | 50 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 1 | 0 |
Golgi | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 2 | 0 | – | 0 | 2 | 6 | 31 | 38 | 4 | 2 | 4 | 0 | 1 | 0 | 0 | 1 | 1 |
Mitochondria | 1 | 7 | 6 | 26 | 24 | 62 | 121 | 91 | 111 | – | 130 | 92 | 8 | 9 | 17 | 4 | 3 | 6 | 2 | 0 | 6 | 2 | 5 | 3 |
Nucleus | 1 | 0 | 0 | 2 | 1 | 0 | 3 | 3 | 1 | – | 0 | 1 | 0 | 0 | 0 | 2 | 3 | 2 | 0 | 1 | 1 | 0 | 0 | 0 |
Nuclear Periphery | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | – | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 2 | 12 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | – | 0 | 0 | 0 | 3 | 9 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vac/Vac Membrane | 2 | 1 | 18 | 8 | 20 | 27 | 47 | 102 | 32 | – | 14 | 36 | 0 | 2 | 3 | 8 | 17 | 5 | 3 | 7 | 9 | 4 | 7 | 9 |
Unique Cell Count | 151 | 215 | 228 | 219 | 282 | 250 | 376 | 385 | 231 | 244 | 239 | 114 | 205 | 248 | 329 | 332 | 300 | 163 | 244 | 247 | 158 | 207 | 228 | |
Labelled Cell Count | 154 | 226 | 253 | 250 | 312 | 294 | 450 | 453 | 295 | 304 | 287 | 127 | 246 | 307 | 357 | 357 | 326 | 163 | 244 | 247 | 158 | 207 | 228 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 11.0 | 11.7 | 9.5 | 8.8 | 9.5 | 7.8 | 7.8 | 8.3 | 7.3 | – | 7.0 | 7.5 | 10.8 | 11.4 | 11.7 | 16.4 | 15.9 | 16.2 | 10.0 | 10.1 | 10.7 |
Std Deviation (1e-4) | 1.5 | 2.4 | 1.3 | 1.0 | 1.2 | 1.2 | 1.3 | 1.3 | 1.3 | – | 1.4 | 1.4 | 1.8 | 1.4 | 1.6 | 2.9 | 2.9 | 2.8 | 1.4 | 1.5 | 1.4 |
Intensity Change (Log2) | – | – | – | -0.11 | -0.01 | -0.3 | -0.28 | -0.2 | -0.38 | – | -0.45 | -0.35 | 0.18 | 0.26 | 0.29 | 0.78 | 0.74 | 0.77 | 0.07 | 0.08 | 0.16 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 2.2 | 2.9 | – | 0 | 3.1 | 0 | 4.7 | 3.6 | 0 | 0 | 0 |
Bud | 0 | 0 | 1.7 | 0 | 0.7 | 0 | – | 0 | 0 | 0 | 2.3 | 3.3 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0.1 | -0.3 | 0 | -1.6 | -2.1 | -1.3 | – | -0.4 | -0.1 | 0 | -1.4 | -1.8 | 1.4 | 1.0 | 3.7 |
Cytoplasm | -0.8 | -3.0 | -6.8 | -8.2 | -9.9 | -10.1 | – | -11.2 | -10.8 | -4.8 | -8.1 | -10.7 | -0.3 | -0.4 | -1.9 |
Endoplasmic Reticulum | 0 | 2.0 | 0 | 2.1 | 0 | 2.5 | – | 5.0 | 3.7 | 5.7 | 3.3 | 3.6 | 2.5 | 0 | 0 |
Endosome | 0 | 0 | 0 | 0 | 2.3 | 0 | – | 0 | 0 | 0 | 4.7 | 6.7 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 6.1 | 6.2 | 0 | 0 | 0 |
Mitochondria | 3.8 | 2.8 | 6.9 | 8.6 | 6.9 | 11.2 | – | 12.1 | 9.5 | 0 | 1.0 | 2.1 | 0 | 0 | -0.5 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 3.4 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | -1.9 | -0.3 | 1.1 | 1.8 | 5.6 | 2.0 | – | -0.9 | 2.4 | -3.1 | -3.4 | -3.5 | -3.0 | -1.3 | -3.5 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 7.8289 | 11.4378 | 10.1369 | 8.5425 | 8.1503 | 10.0127 | 7.4177 | 10.5373 | 9.4806 | 8.946 | 8.2846 | 9.1946 | 6.3507 | 9.3889 | 8.5362 | 7.371 | 7.1834 | 8.2778 |
Actin | 0.0153 | 0.002 | 0.0022 | 0.0017 | 0.0106 | 0.0013 | 0.0185 | 0.0011 | 0.0038 | 0.0046 | 0.0063 | 0.006 | 0.0281 | 0.0017 | 0.0062 | 0.0386 | 0.0307 | 0.0016 |
Bud | 0.0008 | 0.001 | 0.0011 | 0.0015 | 0.001 | 0.0007 | 0.0011 | 0.0008 | 0.0012 | 0.001 | 0.0019 | 0.0005 | 0.0009 | 0.0006 | 0.0009 | 0.0004 | 0.0049 | 0.0004 |
Bud Neck | 0.0008 | 0.0004 | 0.0008 | 0.001 | 0.0029 | 0.0015 | 0.001 | 0.0003 | 0.0008 | 0.0008 | 0.0013 | 0.0012 | 0.0043 | 0.0002 | 0.0005 | 0.0026 | 0.0011 | 0.001 |
Bud Periphery | 0.0011 | 0.0013 | 0.0008 | 0.0008 | 0.0015 | 0.0008 | 0.0013 | 0.0003 | 0.0007 | 0.0007 | 0.0018 | 0.0006 | 0.0009 | 0.0002 | 0.0004 | 0.0002 | 0.0117 | 0.0003 |
Bud Site | 0.0018 | 0.0024 | 0.0036 | 0.0009 | 0.0015 | 0.0002 | 0.0042 | 0.0015 | 0.0034 | 0.0004 | 0.008 | 0.0002 | 0.0043 | 0.0018 | 0.0009 | 0.0006 | 0.0038 | 0.0001 |
Cell Periphery | 0.0011 | 0.0011 | 0.0008 | 0.0002 | 0.0007 | 0.0004 | 0.0055 | 0.0002 | 0.0004 | 0.0002 | 0.0002 | 0.0002 | 0.0004 | 0.0002 | 0.0002 | 0.0001 | 0.0003 | 0.0001 |
Cytoplasm | 0.6561 | 0.8896 | 0.7725 | 0.7223 | 0.6281 | 0.7579 | 0.5945 | 0.8988 | 0.7762 | 0.6673 | 0.694 | 0.7513 | 0.5746 | 0.8974 | 0.7764 | 0.8148 | 0.5732 | 0.8093 |
Cytoplasmic Foci | 0.0338 | 0.0188 | 0.0206 | 0.0298 | 0.0484 | 0.0235 | 0.0462 | 0.0158 | 0.0317 | 0.0293 | 0.0478 | 0.0255 | 0.0546 | 0.0159 | 0.0291 | 0.0273 | 0.0402 | 0.0235 |
Eisosomes | 0.0003 | 0 | 0 | 0 | 0.0006 | 0 | 0.0005 | 0 | 0 | 0 | 0.0001 | 0.0001 | 0.0007 | 0 | 0 | 0.0001 | 0.0001 | 0 |
Endoplasmic Reticulum | 0.0942 | 0.0257 | 0.05 | 0.0242 | 0.0443 | 0.0615 | 0.0773 | 0.0197 | 0.034 | 0.1059 | 0.0572 | 0.0784 | 0.0543 | 0.0212 | 0.0514 | 0.012 | 0.0122 | 0.0332 |
Endosome | 0.0867 | 0.0323 | 0.0821 | 0.1194 | 0.1457 | 0.0927 | 0.1178 | 0.0343 | 0.088 | 0.0938 | 0.0958 | 0.0864 | 0.1101 | 0.0393 | 0.0852 | 0.0765 | 0.2103 | 0.0844 |
Golgi | 0.0196 | 0.0071 | 0.0131 | 0.0121 | 0.021 | 0.0178 | 0.0368 | 0.0089 | 0.0264 | 0.0287 | 0.0273 | 0.0208 | 0.0323 | 0.0076 | 0.0208 | 0.0075 | 0.0482 | 0.0223 |
Lipid Particles | 0.0123 | 0.0001 | 0.0004 | 0.0013 | 0.0082 | 0.0001 | 0.0228 | 0.0001 | 0.0012 | 0.0126 | 0.0042 | 0.0008 | 0.0214 | 0.001 | 0.0005 | 0.0008 | 0.0029 | 0.0004 |
Mitochondria | 0.0201 | 0.008 | 0.0227 | 0.007 | 0.025 | 0.0298 | 0.0346 | 0.0103 | 0.0148 | 0.0318 | 0.0394 | 0.0173 | 0.0558 | 0.0056 | 0.0125 | 0.0017 | 0.0276 | 0.016 |
None | 0.0097 | 0.0016 | 0.0021 | 0.0009 | 0.0056 | 0.0014 | 0.0025 | 0.0007 | 0.001 | 0.0012 | 0.0009 | 0.0017 | 0.0041 | 0.001 | 0.0009 | 0.0016 | 0.0012 | 0.0008 |
Nuclear Periphery | 0.0079 | 0.0016 | 0.0033 | 0.0013 | 0.0059 | 0.0013 | 0.0061 | 0.0006 | 0.0026 | 0.003 | 0.002 | 0.0015 | 0.0177 | 0.0016 | 0.0025 | 0.0014 | 0.0048 | 0.0011 |
Nucleolus | 0.0004 | 0 | 0 | 0 | 0.0008 | 0 | 0.0004 | 0 | 0.0001 | 0 | 0.0002 | 0 | 0.0004 | 0.0001 | 0 | 0 | 0.0005 | 0 |
Nucleus | 0.0019 | 0.0008 | 0.0012 | 0.0009 | 0.0026 | 0.0009 | 0.0017 | 0.0006 | 0.0009 | 0.0008 | 0.0011 | 0.0008 | 0.0075 | 0.0008 | 0.0008 | 0.0011 | 0.0044 | 0.0006 |
Peroxisomes | 0.0112 | 0.0002 | 0.0001 | 0.0591 | 0.0243 | 0.0001 | 0.0102 | 0.0001 | 0.0013 | 0.0051 | 0.003 | 0.0004 | 0.0135 | 0.0002 | 0.0018 | 0.0014 | 0.0022 | 0.0001 |
Punctate Nuclear | 0.0064 | 0.0001 | 0.0001 | 0.0001 | 0.0045 | 0.0001 | 0.0022 | 0.0001 | 0.0003 | 0.0003 | 0.0007 | 0.0004 | 0.0033 | 0.0003 | 0.0002 | 0.0017 | 0.0007 | 0.0001 |
Vacuole | 0.0143 | 0.004 | 0.0176 | 0.0112 | 0.0108 | 0.0052 | 0.0112 | 0.0046 | 0.008 | 0.0087 | 0.0043 | 0.0035 | 0.0068 | 0.0023 | 0.0061 | 0.007 | 0.0115 | 0.0025 |
Vacuole Periphery | 0.0039 | 0.0017 | 0.005 | 0.0045 | 0.0061 | 0.0029 | 0.0033 | 0.0011 | 0.0031 | 0.0038 | 0.0027 | 0.0025 | 0.0039 | 0.001 | 0.0027 | 0.0026 | 0.0077 | 0.0022 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 76.3753 | 54.8674 | 53.8772 | 63.7242 | 75.9882 | 62.7269 | 72.5782 | 72.3519 | 70.3174 | 75.0762 |
Translational Efficiency | 1.0616 | 1.28 | 1.3316 | 0.9903 | 1.058 | 1.4855 | 0.9779 | 1.0566 | 1.089 | 1.1015 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
2392 | 1794 | 744 | 1620 | 205 | 1591 | 1761 | 1466 | 2597 | 3385 | 2505 | 3086 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 1082.66 | 1232.71 | 1434.59 | 1492.41 | 1220.89 | 1148.97 | 1411.64 | 1393.79 | 1093.57 | 1193.35 | 1418.46 | 1445.56 |
Standard Deviation | 144.94 | 196.75 | 288.07 | 201.71 | 157.94 | 182.44 | 183.32 | 219.44 | 150.69 | 194.70 | 219.96 | 216.01 |
Intensity Change Log 2 | — | 0.187253 | 0.406058 | 0.463064 | — | -0.087592 | 0.209439 | 0.191080 | — | 0.048121 | 0.305193 | 0.325312 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.000197 | 0.000353 | 0.000972 | 0.004725 | 0.001196 | 0.000506 | 0.000835 | 0.003291 | 0.000276 | 0.000425 | 0.000876 | 0.004044 |
Bud Neck | 0.000534 | 0.000483 | 0.003193 | 0.002490 | 0.000819 | 0.000833 | 0.001019 | 0.002084 | 0.000557 | 0.000647 | 0.001665 | 0.002297 |
Bud Site | 0.016885 | 0.020929 | 0.035649 | 0.054213 | 0.041518 | 0.022100 | 0.016933 | 0.052182 | 0.018830 | 0.021479 | 0.022492 | 0.053248 |
Cell Periphery | 0.000266 | 0.000277 | 0.002013 | 0.001377 | 0.001110 | 0.000262 | 0.000238 | 0.001515 | 0.000332 | 0.000270 | 0.000766 | 0.001442 |
Cytoplasm | 0.513095* | 0.624167* | 0.645088* | 0.619015* | 0.367355* | 0.555381* | 0.730265* | 0.663064* | 0.501591* | 0.591837* | 0.704967* | 0.639940* |
Cytoplasmic Foci | 0.289687* | 0.241185* | 0.059859 | 0.130457 | 0.391522* | 0.292279* | 0.103667 | 0.101872 | 0.297726* | 0.265200* | 0.090656 | 0.116878 |
Eisosomes | 0.000016 | 0.000010 | 0.000007 | 0.000018 | 0.000025 | 0.000019 | 0.000006 | 0.000014 | 0.000017 | 0.000015 | 0.000006 | 0.000016 |
Endoplasmic Reticulum | 0.038333 | 0.016429 | 0.010983 | 0.002800 | 0.082656 | 0.022218 | 0.009734 | 0.002367 | 0.041831 | 0.019150 | 0.010105 | 0.002594 |
Endosome | 0.011609 | 0.010841 | 0.017951 | 0.020471 | 0.021769 | 0.015347 | 0.012316 | 0.016991 | 0.012411 | 0.012959 | 0.013990 | 0.018818 |
Golgi | 0.042313 | 0.036230 | 0.045244 | 0.032871 | 0.045904 | 0.034764 | 0.013623 | 0.028390 | 0.042597 | 0.035540 | 0.023015 | 0.030742 |
Lipid Particles | 0.002539 | 0.002027 | 0.012712 | 0.015474 | 0.003437 | 0.003003 | 0.006370 | 0.012442 | 0.002610 | 0.002486 | 0.008254 | 0.014034 |
Mitochondria | 0.006609 | 0.001692 | 0.000170 | 0.000537 | 0.000467 | 0.003018 | 0.000282 | 0.001912 | 0.006124 | 0.002315 | 0.000249 | 0.001190 |
Mitotic Spindle | 0.001850 | 0.001869 | 0.012866 | 0.021702 | 0.002686 | 0.002858 | 0.008506 | 0.013913 | 0.001916 | 0.002334 | 0.009801 | 0.018002 |
None | 0.002820 | 0.000672 | 0.001122 | 0.000161 | 0.000120 | 0.001176 | 0.000227 | 0.000362 | 0.002607 | 0.000909 | 0.000493 | 0.000257 |
Nuclear Periphery | 0.000260 | 0.000050 | 0.000823 | 0.000125 | 0.000407 | 0.000088 | 0.000284 | 0.000111 | 0.000272 | 0.000067 | 0.000444 | 0.000118 |
Nuclear Periphery Foci | 0.001766 | 0.001537 | 0.030712 | 0.002371 | 0.004282 | 0.001042 | 0.004791 | 0.002398 | 0.001965 | 0.001304 | 0.012490 | 0.002384 |
Nucleolus | 0.000146 | 0.000049 | 0.000096 | 0.000107 | 0.000097 | 0.000231 | 0.000089 | 0.000163 | 0.000142 | 0.000135 | 0.000091 | 0.000134 |
Nucleus | 0.003160 | 0.001802 | 0.006012 | 0.001933 | 0.001994 | 0.003002 | 0.004500 | 0.002184 | 0.003068 | 0.002366 | 0.004949 | 0.002052 |
Peroxisomes | 0.000372 | 0.000582 | 0.000203 | 0.004355 | 0.000434 | 0.000643 | 0.000611 | 0.003648 | 0.000377 | 0.000611 | 0.000490 | 0.004019 |
Vacuole | 0.019992 | 0.019059 | 0.081269 | 0.075617 | 0.015620 | 0.021904 | 0.077391 | 0.080306 | 0.019647 | 0.020396 | 0.078543 | 0.077844 |
Vacuole Periphery | 0.047550 | 0.019758 | 0.033056 | 0.009182 | 0.016584 | 0.019324 | 0.008310 | 0.010791 | 0.045106 | 0.019554 | 0.015659 | 0.009946 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | -4.89 | -7.04 | -6.35 | -6.12 | -5.16 | 4.21 | 1.30 | -3.57 | -4.94 | -4.05 | -4.67 | -4.13 | -8.19 | -7.85 | -6.49 |
Bud Neck | 1.02 | -11.09 | -12.91 | -13.07 | 2.36 | -0.13 | -2.24 | -8.23 | -8.99 | -7.62 | -1.96 | -12.95 | -16.90 | -15.79 | -5.07 |
Bud Site | -3.52 | -6.40 | -15.39 | -13.33 | -5.20 | 3.98 | 5.04 | -1.90 | -10.85 | -12.79 | -2.59 | -2.70 | -18.35 | -17.12 | -15.00 |
Cell Periphery | -0.63 | -8.80 | -6.82 | -6.72 | 2.72 | 3.62 | 3.74 | -1.16 | -7.05 | -7.24 | 2.60 | -6.61 | -9.09 | -9.73 | -4.82 |
Cytoplasm | -11.73 | -9.79 | -9.26 | 1.29 | 2.24 | -9.06 | -17.59 | -13.50 | -8.50 | 6.83 | -11.33 | -23.96 | -15.13 | -4.83 | 8.51 |
Cytoplasmic Foci | 6.97 | 41.94 | 24.39 | 15.88 | -13.94 | 5.90 | 17.84 | 17.61 | 27.43 | -0.13 | 5.51 | 40.73 | 33.05 | 30.01 | -7.60 |
Eisosomes | 5.09 | 9.27 | -1.20 | -4.84 | -7.45 | 1.90 | 6.32 | 3.67 | 3.57 | -6.64 | 1.93 | 11.40 | 0.57 | -1.22 | -9.93 |
Endoplasmic Reticulum | 8.43 | 10.34 | 16.10 | 9.33 | 5.26 | 4.81 | 5.87 | 6.48 | 9.71 | 5.83 | 8.90 | 13.10 | 17.41 | 13.41 | 7.54 |
Endosome | 1.09 | -4.90 | -7.27 | -7.53 | -1.41 | 2.70 | 4.05 | 1.84 | -1.27 | -3.66 | -0.85 | -2.45 | -7.05 | -6.25 | -4.88 |
Golgi | 3.50 | -0.71 | 4.79 | 2.04 | 3.84 | 3.03 | 9.16 | 4.65 | 3.22 | -6.22 | 4.86 | 12.96 | 7.23 | 3.71 | -4.05 |
Lipid Particles | 2.63 | -7.94 | -12.95 | -13.40 | -1.72 | 0.80 | -5.42 | -7.87 | -8.68 | -5.27 | 0.60 | -12.05 | -15.60 | -15.68 | -6.62 |
Mitochondria | 7.67 | 11.26 | 10.32 | 3.61 | -2.33 | -4.72 | 1.25 | -2.19 | 1.50 | -2.49 | 6.31 | 11.02 | 8.23 | 2.83 | -2.87 |
Mitotic Spindle | -0.03 | -8.38 | -8.58 | -8.53 | -3.78 | -0.28 | -4.86 | -5.77 | -5.43 | -2.72 | -0.87 | -8.49 | -10.48 | -9.94 | -4.90 |
None | 5.98 | 2.63 | 7.54 | 6.87 | 1.67 | -4.05 | -3.08 | -1.82 | 2.84 | -1.02 | 4.87 | 5.84 | 7.13 | 4.62 | 1.29 |
Nuclear Periphery | 2.93 | -3.67 | 1.63 | -2.39 | 4.92 | 0.91 | 0.33 | 0.77 | -1.58 | 2.24 | 2.85 | -1.92 | 1.83 | -2.86 | 4.94 |
Nuclear Periphery Foci | 0.61 | -11.80 | -1.88 | -2.94 | 11.48 | 1.50 | -0.34 | 0.75 | -3.50 | 3.99 | 1.82 | -12.18 | -1.51 | -4.56 | 11.80 |
Nucleolus | 7.74 | 2.44 | 1.81 | -4.86 | -0.86 | -1.13 | 0.08 | -0.84 | 0.62 | -2.13 | 0.12 | 3.48 | -0.06 | -0.14 | -2.43 |
Nucleus | 8.73 | -4.99 | 5.96 | -2.10 | 6.63 | -1.20 | -3.24 | -0.60 | 1.85 | 8.58 | 3.84 | -7.82 | 4.67 | 0.50 | 11.33 |
Peroxisomes | -0.94 | 4.08 | -8.79 | -7.46 | -9.15 | -3.35 | -2.45 | -8.20 | -7.64 | -7.63 | -1.92 | -2.26 | -12.05 | -10.50 | -11.53 |
Vacuole | 0.54 | -11.83 | -14.92 | -14.62 | 0.71 | -1.83 | -14.03 | -13.14 | -14.04 | -1.00 | -0.56 | -20.59 | -20.73 | -20.26 | -0.23 |
Vacuole Periphery | 12.25 | 4.21 | 18.99 | 7.83 | 7.40 | -0.89 | 2.95 | 2.11 | 5.52 | -1.88 | 12.81 | 14.39 | 18.88 | 9.40 | 4.76 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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