Standard name
Human Ortholog
Description Mismatch repair protein; forms dimers with Msh2p that mediate repair of insertion or deletion mutations and removal of nonhomologous DNA ends, contains a PCNA (Pol30p) binding motif required for genome stability
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0 0.05 0.05 0.06 0 0.06 0.06 0.05 0.08 0.17 0.2 0.06 0.07 0.11 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.05 0.27 0.53 0.56 0.38 0.69 0.68 0.63 0.82 0.84 0.84 0.9 0 0 0.1 0.07 0.05 0.09 0 0 0 0 0 0
Nucleus 0.92 0.85 0.86 0.86 0.82 0.75 0.66 0.66 0.61 0.56 0.57 0.46 0.9 0.82 0.75 0.87 0.84 0.78 0.88 0.87 0.86 0.86 0.74 0.59
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0.07 0 0 0 0 0 0 0 0 0 0 0 0 0 0.09 0.11 0.11 0.06 0.08 0.12 0.09 0.17 0.26
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 1 0 0 0
Bud 0 0 0 0 1 4 5 5 11 8 6 6 0 0 1 0 0 0 1 0 0 0 5 1
Bud Neck 0 0 0 0 0 0 1 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 5 4 11 13 11 17 17 16 7 0 0 1 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 1 1 0 0 1 0 0 0 0 0 0
Cytoplasm 7 3 4 5 18 22 30 45 24 39 24 30 23 65 92 20 16 36 0 1 0 0 3 3
Endoplasmic Reticulum 2 0 1 0 0 0 0 0 0 0 0 0 0 2 0 10 7 8 0 0 0 0 0 1
Endosome 1 0 0 0 1 0 0 0 0 0 0 0 1 0 1 2 2 4 3 0 0 0 3 6
Golgi 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 2 0 1 1 1 0 1
Mitochondria 9 35 113 161 157 333 439 481 455 525 359 517 10 14 48 21 10 29 1 1 0 1 3 12
Nucleus 180 111 182 248 334 360 427 503 335 351 244 262 264 322 344 280 183 253 176 111 184 159 299 218
Nuclear Periphery 0 0 0 0 1 2 2 3 1 4 1 1 1 0 0 4 1 1 0 0 0 0 0 1
Nucleolus 0 0 0 2 2 0 4 3 4 2 3 2 0 2 0 0 0 0 0 0 0 0 11 18
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 1 1 1 2 2 5 3 5 2 2 0 0 0 0 0 5 0 0 0 0 4 2
Vac/Vac Membrane 6 9 1 2 10 9 14 18 10 4 8 11 2 8 14 28 25 37 11 9 25 17 67 98
Unique Cell Count 195 130 212 287 409 483 643 764 553 624 425 573 292 392 460 323 219 326 200 128 214 185 403 371
Labelled Cell Count 206 158 302 424 529 743 937 1074 860 955 663 839 301 414 502 366 244 376 200 128 214 185 403 371


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.8 4.3 3.6 3.5 3.7 3.3 3.2 3.2 2.8 2.8 2.8 2.5 5.2 5.2 4.5 6.3 6.7 7.0 5.3 4.7 5.4
Std Deviation (1e-4) 1.1 0.6 0.7 0.7 0.6 0.7 0.8 0.7 0.7 0.6 0.7 0.6 1.1 1.3 1.2 1.3 1.6 1.9 1.0 1.0 1.3
Intensity Change (Log2) – – – -0.05 0.03 -0.14 -0.18 -0.16 -0.4 -0.4 -0.4 -0.53 0.52 0.52 0.3 0.8 0.89 0.95 0.53 0.37 0.59


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – 0 0 0
Bud Neck – – – – – – – – – – – – 0 0 0
Bud Site – – – – – – – – – – – – 0 0 0
Cell Periphery – – – – – – – – – – – – 0 0 0
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – 0 0 0
Endosome – – – – – – – – – – – – 0 0 0
Golgi – – – – – – – – – – – – 0 0 0
Mitochondria – – – – – – – – – – – – 0 0 0
Nuclear Periphery – – – – – – – – – – – – 0 0 0
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – 0 0 0
Peroxisomes – – – – – – – – – – – – 0 0 0
SpindlePole – – – – – – – – – – – – 0 0 0
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – 0 0 0
Cytoplasm – – – – – – – – – – – – 0 0 0
Nucleus – – – – – – – – – – – – 0 0 0
Vacuole – – – – – – – – – – – – 4.1 4.8 4.8

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 1.1291 1.1864 1.1174 1.0663 0.5704 1.063 1.5019 1.9036 1.6092 1.5148 1.1378 1.3396 0.6493 0.8256 0.6191 0.5494 0.6708 0.3452
Actin 0.0326 0.0001 0.0241 0.0047 0.0076 0.0135 0.0408 0.0008 0.0022 0.0708 0.0077 0 0.0174 0 0.0033 0.0006 0.0004 0.0003
Bud 0.0006 0 0.0005 0.0001 0.0002 0.0001 0.001 0.0001 0.0002 0.0002 0 0 0.0003 0 0.0001 0 0.0001 0.0001
Bud Neck 0.0104 0.003 0.0054 0.0005 0.0065 0.004 0.0022 0.0002 0.0004 0.0005 0.0003 0.001 0.0016 0.0002 0.0001 0.0002 0.0004 0.0012
Bud Periphery 0.0016 0 0.0014 0.0001 0.0007 0.0003 0.0039 0.0001 0.0003 0.0002 0.0001 0 0.001 0 0.0002 0 0.0001 0.0001
Bud Site 0.0035 0.0003 0.0035 0.0002 0.0017 0.0003 0.0054 0.0006 0.0014 0.0007 0.0001 0 0.0098 0.0002 0.0002 0 0.0002 0.0002
Cell Periphery 0.0008 0 0.0004 0 0.0005 0.0001 0.0007 0 0.0002 0.0001 0 0 0.0007 0 0 0 0 0
Cytoplasm 0.0112 0.0148 0.0046 0.0103 0.0029 0.0016 0.0055 0.0342 0.0122 0.0014 0.0051 0.0008 0.0062 0.0081 0.0034 0.0041 0.0197 0.0024
Cytoplasmic Foci 0.0063 0.0001 0.0058 0.0039 0.0023 0.0015 0.0088 0.0023 0.0044 0.0043 0.0059 0 0.0067 0.0005 0.01 0.0001 0.0066 0.0009
Eisosomes 0.0007 0 0.0002 0.0002 0.0003 0.0001 0.0004 0 0.0001 0.0003 0.0002 0 0.0002 0 0.0002 0 0 0
Endoplasmic Reticulum 0.0115 0.0006 0.0013 0.001 0.0006 0.0005 0.0046 0.0012 0.0008 0.0017 0.001 0 0.0035 0.0002 0.0005 0.0003 0.0014 0.0001
Endosome 0.0209 0.0004 0.0045 0.005 0.007 0.0034 0.0228 0.0015 0.006 0.0094 0.0069 0.0001 0.0186 0.0006 0.0031 0.0002 0.0241 0.0007
Golgi 0.0097 0 0.0023 0.0048 0.0044 0.0033 0.0049 0.0031 0.0012 0.0104 0.0023 0 0.0054 0 0.0011 0 0.0116 0.0002
Lipid Particles 0.0227 0 0.0035 0.0292 0.0135 0.0106 0.0087 0.0012 0.0028 0.0072 0.0181 0 0.0194 0.0002 0.0004 0 0.0032 0.0005
Mitochondria 0.0152 0.0002 0.0049 0.0083 0.0088 0.0022 0.0218 0.0007 0.0011 0.0026 0.0007 0.0001 0.0033 0.0002 0.0095 0.0006 0.0025 0.0002
None 0.0116 0.0123 0.0029 0.0028 0.001 0.0003 0.0206 0.0231 0.024 0.0009 0.0221 0.0005 0.0031 0.0335 0.0252 0.0023 0.0784 0.0128
Nuclear Periphery 0.0413 0.0124 0.0081 0.0198 0.0075 0.0063 0.0246 0.0075 0.0066 0.0139 0.0329 0.002 0.0099 0.0026 0.0142 0.0261 0.0382 0.0025
Nucleolus 0.0115 0.0065 0.0079 0.0034 0.0341 0.0152 0.0051 0.0051 0.0043 0.0039 0.0051 0.0101 0.0042 0.0139 0.0049 0.0045 0.0057 0.0116
Nucleus 0.7629 0.9381 0.9019 0.8517 0.8276 0.9265 0.7842 0.9097 0.9198 0.8643 0.8654 0.9662 0.874 0.9236 0.9177 0.9534 0.7814 0.9506
Peroxisomes 0.0048 0 0.0034 0.0021 0.0031 0.0043 0.0049 0.002 0.0004 0.0047 0.0029 0 0.0045 0 0.0008 0 0.0006 0.0013
Punctate Nuclear 0.0119 0.0105 0.0112 0.05 0.0669 0.0046 0.011 0.0054 0.0055 0.0019 0.0219 0.0189 0.005 0.0155 0.0041 0.0068 0.0234 0.0141
Vacuole 0.005 0.0004 0.0015 0.0004 0.002 0.0008 0.0119 0.0011 0.0052 0.0002 0.0007 0.0001 0.004 0.0006 0.0005 0.0001 0.0011 0.0002
Vacuole Periphery 0.003 0.0001 0.0007 0.0015 0.0007 0.0003 0.0061 0.0002 0.0009 0.0003 0.0009 0 0.0013 0.0001 0.0004 0.0003 0.0007 0

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 12.8135 8.7119 7.9011 10.6363 11.6995 12.626 9.2979 11.2298 8.1606 13.5094
Translational Efficiency 0.7328 0.7284 0.7671 0.7735 0.7418 0.662 0.8169 0.5044 0.7582 0.6499

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✔
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
2138 830 92 472 1550 1077 1437 2761 3688 1907 1529 3233

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 637.98 764.55 1026.41 943.99 654.09 786.54 821.70 931.64 644.75 776.97 834.02 933.44
Standard Deviation 73.94 107.86 129.41 119.62 103.42 106.29 122.37 121.26 87.91 107.53 132.10 121.10
Intensity Change Log 2 — 0.261100 0.686024 0.565260 — 0.266031 0.329123 0.510283 — 0.263598 0.516366 0.537691

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000017 0.000345 0.001510 0.001329 0.000087 0.000217 0.000919 0.000337 0.000047 0.000273 0.000954 0.000482
Bud Neck 0.001481 0.003682 0.002517 0.015331 0.002286 0.005598 0.015833 0.021238 0.001819 0.004764 0.015031 0.020376
Bud Site 0.000257 0.001378 0.001113 0.005569 0.000637 0.001819 0.009437 0.004620 0.000417 0.001627 0.008936 0.004758
Cell Periphery 0.000103 0.000106 0.000147 0.000084 0.000133 0.000184 0.000355 0.000129 0.000115 0.000150 0.000343 0.000122
Cytoplasm 0.136865* 0.018865 0.008826 0.019663 0.072355 0.018607 0.010452 0.016628 0.109753* 0.018719 0.010354 0.017071
Cytoplasmic Foci 0.009762 0.004716 0.000301 0.000223 0.004306 0.005162 0.001259 0.000566 0.007469 0.004968 0.001202 0.000516
Eisosomes 0.000014 0.000023 0.000042 0.000025 0.000015 0.000020 0.000030 0.000018 0.000014 0.000021 0.000031 0.000019
Endoplasmic Reticulum 0.000492 0.005762 0.012905 0.008226 0.001738 0.005838 0.010467 0.005225 0.001016 0.005805 0.010613 0.005663
Endosome 0.000414 0.001280 0.000513 0.000697 0.000427 0.001340 0.000861 0.001150 0.000420 0.001314 0.000840 0.001084
Golgi 0.000090 0.001097 0.000100 0.000196 0.000111 0.002405 0.000581 0.000881 0.000099 0.001836 0.000552 0.000781
Lipid Particles 0.000610 0.001267 0.000518 0.000341 0.000668 0.001293 0.000543 0.000129 0.000634 0.001282 0.000542 0.000160
Mitochondria 0.000496 0.004555 0.000854 0.004971 0.001604 0.003334 0.004398 0.004351 0.000961 0.003865 0.004184 0.004442
Mitotic Spindle 0.000025 0.005655 0.000045 0.028622 0.001365 0.004017 0.002711 0.008717 0.000588 0.004730 0.002550 0.011623
None 0.013443 0.004729 0.001133 0.007582 0.023977 0.002333 0.007596 0.003370 0.017870 0.003376 0.007207 0.003985
Nuclear Periphery 0.000516 0.001101 0.000555 0.001077 0.000624 0.001908 0.000934 0.001925 0.000561 0.001557 0.000911 0.001801
Nuclear Periphery Foci 0.000191 0.000534 0.000586 0.000224 0.000486 0.000202 0.000312 0.000166 0.000315 0.000347 0.000329 0.000175
Nucleolus 0.002159 0.004886 0.001600 0.001458 0.004264 0.002879 0.003416 0.000905 0.003044 0.003753 0.003307 0.000985
Nucleus 0.830280* 0.933114* 0.959135* 0.896112* 0.882559* 0.931876* 0.918982* 0.920668* 0.852252* 0.932415* 0.921398* 0.917083*
Peroxisomes 0.000322 0.002750 0.000280 0.001022 0.000624 0.001714 0.000901 0.000159 0.000449 0.002165 0.000864 0.000285
Vacuole 0.002387 0.003547 0.007172 0.007119 0.001506 0.008158 0.009452 0.007974 0.002017 0.006151 0.009314 0.007849
Vacuole Periphery 0.000074 0.000606 0.000146 0.000127 0.000229 0.001093 0.000562 0.000845 0.000139 0.000881 0.000537 0.000740

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -6.23 -7.19 -7.38 -5.46 0.59 -2.95 -7.08 -10.89 -2.74 4.52 -6.51 -8.19 -13.45 -5.01 3.72
Bud Neck -3.68 -2.72 -7.13 -6.10 -6.57 -3.60 -8.97 -18.27 -12.64 -2.81 -5.13 -9.39 -20.37 -15.66 -3.18
Bud Site -3.19 -4.28 -5.06 -3.70 -4.10 -1.77 -6.39 -6.65 -2.47 3.75 -3.02 -6.60 -8.46 -4.08 3.45
Cell Periphery -0.35 -1.98 0.52 0.71 2.21 -0.80 -2.20 2.28 1.33 2.59 -0.98 -2.45 1.42 1.39 2.64
Cytoplasm 23.95 27.06 23.11 0.84 -2.15 12.47 15.91 14.48 0.92 -4.10 26.81 31.05 29.65 1.21 -4.29
Cytoplasmic Foci 3.00 9.01 9.13 3.79 1.79 -0.29 4.28 6.16 4.73 1.99 2.52 8.23 10.77 5.97 2.04
Eisosomes -3.71 -5.34 -9.38 -4.79 0.50 -2.98 -8.52 -3.47 0.67 6.88 -4.87 -10.52 -8.07 -0.43 5.57
Endoplasmic Reticulum -11.33 -9.60 -12.87 -5.68 1.46 -6.47 -11.18 -15.20 0.89 6.41 -11.49 -13.02 -22.46 -0.69 5.89
Endosome -2.12 -0.91 -3.24 1.08 -1.71 -2.89 -2.56 -1.83 1.77 0.50 -3.59 -3.27 -2.67 1.98 0.40
Golgi -2.68 -0.43 -1.61 2.44 -1.45 -2.41 -1.15 -1.11 1.96 0.06 -3.13 -1.30 -1.29 2.30 0.12
Lipid Particles -2.81 0.61 2.22 3.58 0.92 -1.98 0.93 5.29 3.97 4.27 -3.24 0.86 8.17 5.74 4.06
Mitochondria -3.31 -3.04 -3.17 0.59 -2.78 -2.37 -3.30 -3.33 -0.43 0.81 -4.43 -4.55 -5.99 0.26 0.62
Mitotic Spindle -3.15 -1.41 -4.10 -2.85 -4.10 -1.65 -1.61 -4.94 -2.31 -3.46 -3.52 -2.72 -7.34 -3.26 -4.72
None 6.48 11.08 4.22 -0.90 -3.81 9.83 7.07 9.49 -1.83 3.14 12.16 7.45 12.17 -0.77 2.60
Nuclear Periphery -3.65 -0.93 -8.82 -4.67 -8.02 -6.58 -3.62 -13.33 -2.57 -7.40 -7.64 -4.18 -15.70 -5.28 -8.09
Nuclear Periphery Foci -1.53 -2.13 -2.37 -0.68 -0.39 2.61 0.61 2.30 -0.33 1.77 0.23 -0.86 0.72 0.38 1.38
Nucleolus -2.85 1.12 -0.68 0.95 -1.01 2.32 1.13 5.27 3.30 5.12 -0.85 -0.66 4.44 4.15 4.11
Nucleus -12.57 -19.07 -5.05 4.16 7.07 -6.32 -4.30 -5.72 2.04 -0.62 -14.16 -10.50 -12.57 3.58 0.65
Peroxisomes -3.14 0.39 -0.73 2.85 -0.96 -1.75 -0.58 2.35 2.90 2.74 -3.61 -1.31 2.60 4.37 2.56
Vacuole -1.64 -4.24 -6.15 -5.36 -3.95 -4.82 -7.40 -9.62 -1.11 0.73 -4.85 -7.28 -10.93 -4.22 -0.42
Vacuole Periphery -2.45 -3.16 -3.65 2.07 -0.30 -1.98 -2.79 -2.07 1.25 0.14 -2.85 -4.02 -2.92 1.48 0.36
Standard name
Human Ortholog
Description Mismatch repair protein; forms dimers with Msh2p that mediate repair of insertion or deletion mutations and removal of nonhomologous DNA ends, contains a PCNA (Pol30p) binding motif required for genome stability
Localization
Cell Percentages nucleus (82%)
Cell Cycle Regulation No
Subcompartmental Group nucleus-3

Msh3

Msh3


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Msh3-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available