Standard name
Human Ortholog
Description Non-essential INO80 chromatin remodeling complex subunit; preferentially binds DNA ends, protecting them from exonucleatic cleavage; deletion affects telomere maintenance via recombination; related to mammalian high mobility group proteins
DOWNLOAD

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.18 0.43 0.62 0.72 0.62 0.61 0.79 – 0.86 0.88 0.85 0.9 0.78 0.91 0.96 0.16 0.16 0.17 0 0.05 0.05 0.07 0.07 0.08
Endoplasmic Reticulum 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0.07 0 0.05 0 0 0 0 0 0
Endosome 0.09 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.06
Golgi 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.14 0.09 0 0 0 0.14 0 – 0.05 0.05 0.12 0.05 0 0 0 0 0 0.05 0 0 0 0 0 0
Nucleus 0.15 0.09 0.07 0.09 0.08 0.1 0 – 0 0 0 0 0 0 0 0.41 0.3 0.39 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.71 0.62 0.5 0.45 0.49 0.38 0.38 – 0.19 0.16 0.16 0.12 0.28 0.13 0.07 0.56 0.7 0.54 0.85 0.84 0.89 0.83 0.81 0.75
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 1 0 3 0 0 1 2
Bud 0 0 0 0 0 0 0 – 4 5 0 1 0 0 0 0 0 1 4 3 2 1 5 4
Bud Neck 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 2 0 0 1 0
Bud Site 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 – 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 79 161 108 144 147 134 243 – 330 374 152 240 131 308 374 68 53 61 5 20 9 16 28 27
Endoplasmic Reticulum 1 5 1 0 0 1 0 – 0 0 0 0 2 2 0 29 12 19 0 1 0 1 1 3
Endosome 39 3 4 4 2 0 3 – 1 2 1 1 7 10 1 8 10 7 15 10 2 4 18 19
Golgi 0 0 0 0 0 0 0 – 0 0 0 0 0 1 0 8 2 3 2 0 1 0 1 0
Mitochondria 63 33 1 4 3 30 2 – 20 22 21 14 0 2 0 12 2 18 9 10 1 5 10 11
Nucleus 64 34 13 18 19 23 8 – 5 4 0 3 1 0 1 168 100 140 20 2 0 1 1 2
Nuclear Periphery 1 1 0 0 2 2 4 – 1 2 3 4 0 0 1 1 0 3 2 0 0 1 2 3
Nucleolus 10 0 0 0 0 1 0 – 0 0 0 0 0 0 0 0 0 1 4 0 0 0 1 0
Peroxisomes 2 1 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 1 0 0 1 0 0 1 – 0 2 0 0 0 0 0 0 0 1 3 4 1 1 2 2
Vac/Vac Membrane 312 233 87 90 117 84 117 – 74 68 29 33 47 43 26 231 230 193 394 347 175 190 339 240
Unique Cell Count 441 377 175 199 239 220 307 385 424 178 266 167 337 391 413 330 357 466 412 197 229 418 322
Labelled Cell Count 572 471 214 261 290 275 378 435 480 206 296 188 366 403 525 409 448 466 412 197 229 418 322


Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.2 4.9 5.6 5.7 6.1 4.3 5.4 – 4.6 4.7 4.5 4.6 5.3 5.3 5.1 7.8 8.2 7.0 4.9 5.1 5.2
Std Deviation (1e-4) 1.5 1.3 1.9 2.1 1.4 1.2 1.6 – 1.2 1.0 1.4 1.0 1.1 1.7 1.2 2.3 2.3 2.0 1.7 1.7 1.7
Intensity Change (Log2) – – – 0.04 0.12 -0.38 -0.05 – -0.3 -0.26 -0.3 -0.28 -0.09 -0.08 -0.14 0.48 0.55 0.31 -0.2 -0.13 -0.12


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – 0 0 0
Bud Neck – – – – – – – – – – – – 0 0 0
Bud Site – – – – – – – – – – – – 0 0 0
Cell Periphery – – – – – – – – – – – – 0 0 0
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – 0 0 0
Endosome – – – – – – – – – – – – 0 0 0
Golgi – – – – – – – – – – – – 0 0 0
Mitochondria – – – – – – – – – – – – 0 0 0
Nuclear Periphery – – – – – – – – – – – – 0 0 0
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – 0 0 0
Peroxisomes – – – – – – – – – – – – 0 0 0
SpindlePole – – – – – – – – – – – – 0 0 0
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – 0 0 0
Cytoplasm – – – – – – – – – – – – 0 0 0
Nucleus – – – – – – – – – – – – 0 0 0
Vacuole – – – – – – – – – – – – 1.4 4.4 0.9

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 0.51 0.4761 -0.0241 0.1622 -0.1658 0.0212 1.6376 3.0224 2.3813 2.1447 2.752 2.2934 3.2309 4.8031 3.4926 3.9845 2.7828 3.8843
Actin 0.0318 0.0003 0 0.0243 0.0001 0 0.0058 0.0002 0.0005 0.0003 0.0016 0.0007 0.0067 0 0.0003 0 0 0.0003
Bud 0.0009 0.0007 0.0002 0.0014 0.0005 0.0003 0.001 0.001 0.0012 0.0009 0.0007 0.001 0.0005 0.0126 0.0008 0.0007 0.0007 0.0006
Bud Neck 0.0044 0.0015 0.0007 0.0014 0.0016 0.0021 0.0015 0.0022 0.003 0.0012 0.0011 0.0024 0.0024 0.0014 0.0012 0.0026 0.0016 0.0024
Bud Periphery 0.0011 0.001 0.0006 0.0022 0.0016 0.0008 0.0023 0.0022 0.0029 0.0028 0.0018 0.0035 0.0013 0.0136 0.0029 0.0014 0.0014 0.0018
Bud Site 0.0048 0.0062 0.0007 0.0098 0.0016 0.0006 0.0063 0.0025 0.0041 0.0013 0.0013 0.0013 0.0027 0.003 0.0021 0.0053 0.0029 0.0011
Cell Periphery 0.0016 0.0019 0.0011 0.0005 0.0011 0.0012 0.0013 0.0024 0.0025 0.0009 0.0012 0.0021 0.0042 0.0017 0.0016 0.0017 0.0009 0.0024
Cytoplasm 0.0227 0.0288 0.0311 0.0492 0.0235 0.0336 0.0198 0.0173 0.0125 0.0043 0.0122 0.0106 0.0228 0.0211 0.0176 0.0101 0.0167 0.0205
Cytoplasmic Foci 0.03 0.0372 0.0287 0.0144 0.0515 0.0351 0.0302 0.0154 0.0259 0.019 0.034 0.0211 0.0252 0.0133 0.037 0.0094 0.0695 0.0264
Eisosomes 0.0003 0.0001 0 0.0002 0.0001 0 0 0 0.0001 0 0 0 0.0002 0 0.0001 0 0.0001 0
Endoplasmic Reticulum 0.0086 0.0039 0.0035 0.0027 0.002 0.003 0.0057 0.0042 0.0035 0.0011 0.0029 0.002 0.0051 0.0036 0.0024 0.0014 0.0013 0.0033
Endosome 0.2111 0.2359 0.2426 0.4244 0.3659 0.2688 0.2878 0.1627 0.2719 0.2339 0.255 0.2479 0.3092 0.202 0.3149 0.2256 0.4069 0.2829
Golgi 0.0289 0.0066 0.0068 0.0175 0.0119 0.0056 0.0104 0.0037 0.0069 0.0062 0.0116 0.007 0.0072 0.0038 0.007 0.0047 0.009 0.0059
Lipid Particles 0.0209 0.0255 0.0231 0.0202 0.0818 0.0245 0.0267 0.0095 0.0148 0.0173 0.0137 0.0132 0.0194 0.0128 0.0146 0.0292 0.0201 0.0187
Mitochondria 0.0303 0.032 0.051 0.0181 0.017 0.0138 0.0172 0.0132 0.023 0.0215 0.0133 0.0068 0.0175 0.0079 0.0312 0.037 0.0162 0.0207
None 0.0576 0.0181 0.0565 0.004 0.0486 0.0639 0.0144 0.0039 0.0236 0.0015 0.0103 0.0097 0.041 0.021 0.0581 0.0009 0.0053 0.022
Nuclear Periphery 0.0172 0.0083 0.008 0.0056 0.0018 0.0069 0.0094 0.0124 0.0067 0.0023 0.0159 0.0055 0.0077 0.003 0.0047 0.0018 0.0017 0.0056
Nucleolus 0.0033 0.0064 0.0035 0.0011 0.0016 0.0039 0.0074 0.0082 0.0117 0.0032 0.0037 0.0072 0.0089 0.0054 0.0059 0.0053 0.0232 0.0033
Nucleus 0.0068 0.0055 0.0065 0.0256 0.0018 0.0063 0.0162 0.0106 0.0144 0.0041 0.0091 0.0137 0.0088 0.0061 0.0164 0.0042 0.0185 0.0064
Peroxisomes 0.0107 0.0015 0.0016 0.0042 0.0222 0.0029 0.0055 0.0009 0.0039 0.0159 0.0069 0.004 0.0024 0.0013 0.0035 0.0022 0.0042 0.0034
Punctate Nuclear 0.0038 0.0009 0.0006 0.0017 0.0006 0.0007 0.0016 0.0003 0.0011 0.0005 0.0108 0.0013 0.0029 0.0004 0.0008 0.0002 0.0019 0.0012
Vacuole 0.3951 0.4559 0.4057 0.2587 0.3163 0.4327 0.4293 0.6037 0.4525 0.5701 0.5007 0.5226 0.431 0.5866 0.3878 0.5552 0.354 0.4831
Vacuole Periphery 0.1081 0.1219 0.1276 0.1127 0.0467 0.0932 0.1002 0.1236 0.1133 0.0916 0.0919 0.1163 0.0731 0.0793 0.0891 0.1011 0.044 0.0879

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 21.6354 24.1759 38.271 23.325 26.8824 46.8507 23.222 25.8472 41.3372 24.5386
Translational Efficiency 1.9806 1.6774 0.8918 1.9722 1.3737 0.9555 1.6901 1.5021 0.9998 1.4985

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1690 1078 1743 895 1417 1629 1868 1645 3107 2707 3611 2540

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 701.26 752.51 804.09 791.05 704.99 789.64 835.30 857.77 702.96 774.85 820.24 834.26
Standard Deviation 89.11 117.89 97.50 103.23 83.53 165.30 96.53 119.05 86.63 149.36 98.24 118.11
Intensity Change Log 2 — 0.101761 0.197408 0.173819 — 0.163592 0.244692 0.282988 — 0.133090 0.221306 0.229581

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000030 0.000075 0.000190 0.001221 0.000025 0.000058 0.000114 0.000420 0.000028 0.000065 0.000151 0.000702
Bud Neck 0.021546 0.018910 0.036301 0.024609 0.018667 0.021392 0.032956 0.026414 0.020233 0.020404 0.034570 0.025778
Bud Site 0.010540 0.019541 0.017106 0.026089 0.006294 0.016849 0.010929 0.040484 0.008603 0.017921 0.013911 0.035412
Cell Periphery 0.000243 0.000188 0.000099 0.000061 0.000249 0.000228 0.000054 0.000081 0.000246 0.000212 0.000076 0.000074
Cytoplasm 0.072204 0.166257* 0.289135* 0.129249* 0.114518* 0.070297 0.295272* 0.238282* 0.091502 0.108511* 0.292310* 0.199863*
Cytoplasmic Foci 0.027142 0.063854 0.039920 0.106725 0.039003 0.024973 0.043434 0.062827 0.032552 0.040456 0.041738 0.078295
Eisosomes 0.000023 0.000030 0.000042 0.000033 0.000035 0.000020 0.000029 0.000025 0.000029 0.000024 0.000035 0.000028
Endoplasmic Reticulum 0.001271 0.001277 0.004199 0.001112 0.001696 0.002524 0.002735 0.001315 0.001465 0.002027 0.003442 0.001243
Endosome 0.069879 0.078553 0.067583 0.221241* 0.063684 0.072697 0.095933 0.135391 0.067054 0.075029 0.082249 0.165642
Golgi 0.000710 0.001507 0.000628 0.005899 0.000722 0.001295 0.000487 0.005627 0.000716 0.001380 0.000555 0.005723
Lipid Particles 0.001827 0.002223 0.003930 0.003497 0.001794 0.001494 0.002556 0.001907 0.001812 0.001784 0.003219 0.002467
Mitochondria 0.000876 0.001804 0.002519 0.002483 0.000867 0.003347 0.001017 0.003331 0.000872 0.002732 0.001742 0.003032
Mitotic Spindle 0.000292 0.002325 0.016884 0.014587 0.001908 0.000567 0.009200 0.034045 0.001029 0.001267 0.012909 0.027189
None 0.000552 0.000820 0.003643 0.000332 0.001165 0.000443 0.002297 0.002912 0.000832 0.000593 0.002947 0.002003
Nuclear Periphery 0.000263 0.000266 0.005944 0.000723 0.000233 0.000230 0.003371 0.001067 0.000249 0.000244 0.004613 0.000946
Nuclear Periphery Foci 0.000090 0.000323 0.003414 0.002090 0.000236 0.000133 0.003175 0.001197 0.000156 0.000209 0.003290 0.001512
Nucleolus 0.000873 0.001447 0.009896 0.002792 0.000817 0.001079 0.006079 0.001814 0.000848 0.001225 0.007922 0.002159
Nucleus 0.011499 0.028372 0.250289* 0.017419 0.025797 0.017610 0.161985* 0.047715 0.018020 0.021896 0.204609* 0.037040
Peroxisomes 0.000883 0.002795 0.004007 0.019820 0.001294 0.001321 0.005438 0.009400 0.001070 0.001908 0.004747 0.013072
Vacuole 0.768046* 0.594330* 0.241833* 0.414003* 0.713466* 0.742879* 0.320656* 0.380022* 0.743154* 0.683723* 0.282609* 0.391995*
Vacuole Periphery 0.011210 0.015104 0.002438 0.006016 0.007527 0.020563 0.002282 0.005723 0.009530 0.018389 0.002358 0.005826

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -3.48 -11.18 -1.72 -1.64 -1.36 -1.23 -10.91 -7.02 -5.64 -4.58 -2.23 -15.00 -2.86 -2.65 -2.05
Bud Neck 1.24 -7.38 -0.83 -1.87 6.03 -1.24 -6.09 -3.34 -1.78 3.21 -0.06 -9.42 -2.93 -2.61 6.64
Bud Site -4.40 -2.14 -5.73 -1.73 -3.73 -5.88 -3.55 -10.29 -4.86 -8.76 -6.78 -3.33 -11.18 -5.07 -8.86
Cell Periphery 1.47 7.50 8.90 3.74 4.32 0.56 5.61 5.13 9.01 -3.14 1.36 9.02 9.21 8.49 1.15
Cytoplasm -12.39 -27.64 -10.67 0.78 10.97 6.60 -22.36 -17.63 -24.46 3.56 -3.85 -35.72 -22.62 -18.06 8.23
Cytoplasmic Foci -10.46 -5.03 -14.36 -5.19 -11.59 4.99 -0.82 -4.30 -9.65 -4.09 -3.95 -4.32 -13.59 -9.44 -10.86
Eisosomes -2.65 -10.31 -2.77 -0.84 2.89 5.91 1.24 3.03 -4.84 3.04 2.67 -6.08 -0.63 -3.19 5.01
Endoplasmic Reticulum 0.03 -14.60 0.32 0.37 16.76 -2.27 -4.58 1.26 3.68 7.91 -2.56 -13.46 0.98 3.56 16.31
Endosome -1.60 3.52 -11.76 -10.43 -13.61 -2.12 -1.50 -4.92 -3.14 -3.40 -2.38 1.28 -10.99 -9.03 -11.64
Golgi -2.58 1.29 -3.17 -2.36 -3.39 -1.62 1.15 -3.29 -2.63 -3.99 -2.94 1.72 -4.62 -3.51 -5.21
Lipid Particles -1.01 -7.60 -5.19 -3.11 1.23 1.16 -5.43 -1.92 -2.58 4.43 0.14 -9.10 -4.83 -3.81 4.46
Mitochondria -2.52 -3.94 -1.44 -0.20 0.51 -4.04 -0.71 -3.27 1.43 -3.35 -4.79 -3.70 -3.53 0.98 -1.34
Mitotic Spindle -1.73 -9.63 -6.08 -5.06 0.51 1.61 -6.76 -9.97 -10.87 -4.78 -0.34 -11.74 -11.98 -11.81 -3.62
None -0.58 -4.97 0.22 1.77 7.69 1.73 -2.35 -1.43 -2.77 -0.07 0.85 -5.35 -1.56 -2.41 2.21
Nuclear Periphery -0.15 -17.85 -8.15 -8.63 15.14 0.03 -14.57 -13.30 -12.77 8.35 0.04 -22.85 -15.11 -15.27 16.58
Nuclear Periphery Foci -5.52 -10.09 -5.73 -5.19 2.89 1.27 -8.31 -4.79 -5.34 4.36 -1.06 -12.92 -7.49 -7.37 5.51
Nucleolus -0.84 -10.60 -2.48 -2.23 8.09 -0.75 -10.89 -7.18 -4.07 7.97 -0.88 -14.68 -4.97 -4.51 11.69
Nucleus -6.16 -37.56 -7.41 0.35 34.18 3.10 -28.63 -11.46 -13.85 18.37 -2.24 -46.82 -14.80 -11.92 35.03
Peroxisomes -6.11 -7.08 -8.90 -7.60 -6.77 -0.23 -6.16 -8.48 -8.47 -3.79 -4.71 -9.45 -12.36 -11.03 -7.64
Vacuole 15.23 59.48* 27.03 10.20 -16.79 -2.75 38.23* 28.28 32.21* -8.26 7.88 67.95* 41.24 30.57 -17.74
Vacuole Periphery -3.07 16.75 9.18 9.05 -4.33 -10.76 8.89 4.40 14.10 -2.82 -10.23 18.16 9.32 16.62 -4.32
Standard name
Human Ortholog
Description Non-essential INO80 chromatin remodeling complex subunit; preferentially binds DNA ends, protecting them from exonucleatic cleavage; deletion affects telomere maintenance via recombination; related to mammalian high mobility group proteins
Localization
Cell Percentages vacuole (91%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Nhp10

Nhp10


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Nhp10-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available