Standard name | |
---|---|
Human Ortholog | |
Description | Essential alpha-kleisin subunit of the cohesin complex; required for sister chromatid cohesion in mitosis; subject to proteolytic cleavage by separate Esp1p, resulting in dissociation of cohesin from chromatin and the separation of sister chromatids at the mitotic metaphase-to-anaphase transition; apoptosis induces cleavage and translocation of a C-terminal fragment to mitochondria; expression peaks in S phase |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 1 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 2 | 5 | – | 6 | 2 | 5 | 5 | 0 | 0 | 0 | 0 | 0 | 0 | 33 | 23 | 25 | 1 | 3 | 1 |
Bud Neck | 0 | 0 | 2 | 0 | 0 | 0 | 3 | – | 7 | 7 | 4 | 6 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 1 | 0 |
Bud Site | 1 | 1 | 0 | 0 | 0 | 0 | 3 | – | 3 | 4 | 6 | 8 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 97 | 48 | 80 | 91 | 77 | 126 | 173 | – | 127 | 120 | 126 | 153 | 71 | 54 | 83 | 50 | 50 | 56 | 56 | 23 | 42 | 23 | 68 | 108 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 12 | 12 | 7 | 1 | 0 | 0 | 0 | 0 | 0 |
Endosome | 9 | 3 | 5 | 5 | 5 | 13 | 9 | – | 1 | 5 | 3 | 4 | 3 | 5 | 6 | 47 | 41 | 34 | 5 | 1 | 1 | 0 | 4 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3 | 1 | 4 | 0 | 1 | 0 | 1 | 0 |
Mitochondria | 20 | 1 | 0 | 11 | 4 | 29 | 63 | – | 72 | 74 | 146 | 144 | 0 | 0 | 0 | 9 | 7 | 5 | 6 | 0 | 2 | 0 | 1 | 2 |
Nucleus | 95 | 42 | 85 | 75 | 97 | 102 | 110 | – | 48 | 78 | 65 | 93 | 60 | 38 | 53 | 70 | 57 | 58 | 90 | 58 | 80 | 5 | 4 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 2 | 1 | – | 0 | 3 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 41 | 18 | 21 | 41 | 68 | 60 | 78 | – | 62 | 80 | 71 | 98 | 12 | 31 | 43 | 32 | 33 | 38 | 37 | 15 | 26 | 0 | 0 | 0 |
Peroxisomes | 2 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 5 | 1 | 4 | 8 | 1 | 0 | 7 | – | 2 | 13 | 3 | 12 | 2 | 1 | 9 | 4 | 11 | 10 | 33 | 15 | 25 | 0 | 3 | 3 |
Vac/Vac Membrane | 21 | 35 | 22 | 24 | 33 | 35 | 34 | – | 6 | 17 | 19 | 17 | 11 | 9 | 2 | 55 | 50 | 54 | 16 | 11 | 22 | 3 | 5 | 4 |
Unique Cell Count | 259 | 124 | 202 | 225 | 254 | 327 | 419 | 290 | 340 | 350 | 437 | 146 | 122 | 183 | 228 | 215 | 213 | 291 | 153 | 234 | 35 | 100 | 125 | |
Labelled Cell Count | 291 | 149 | 219 | 255 | 285 | 369 | 487 | 335 | 403 | 449 | 540 | 159 | 140 | 197 | 280 | 265 | 263 | 291 | 153 | 234 | 35 | 100 | 125 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 5.2 | 6.9 | 6.9 | 6.4 | 7.4 | 6.0 | 6.1 | – | 4.9 | 5.4 | 4.4 | 4.9 | 7.6 | 8.2 | 8.1 | 9.4 | 9.5 | 9.4 | – | 5.4 | 5.5 |
Std Deviation (1e-4) | 1.9 | 2.8 | 2.1 | 1.9 | 2.1 | 1.8 | 2.4 | – | 1.9 | 2.1 | 1.6 | 1.8 | 2.8 | 3.0 | 3.0 | 3.9 | 4.0 | 3.6 | – | 1.7 | 1.2 |
Intensity Change (Log2) | – | – | – | -0.11 | 0.09 | -0.21 | -0.19 | – | -0.51 | -0.36 | -0.67 | -0.51 | 0.13 | 0.24 | 0.23 | 0.44 | 0.46 | 0.43 | – | -0.37 | -0.34 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 0.2 | -2.1 | -0.2 | 0.4 | – | 0.9 | -1.0 | -0.8 | -1.1 | 1.7 | 0.8 | 1.1 | -4.0 | -3.6 | -2.9 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3.3 | 3.4 | 0 |
Endosome | 0 | 0 | 0.9 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0.5 | 5.8 | 5.4 | 4.7 |
Golgi | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 3.2 | 0 | 4.4 | 5.8 | – | 0 | 7.1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | -1.9 | -0.8 | -2.5 | -4.0 | – | -6.3 | -4.7 | -6.0 | -5.5 | -0.2 | -2.0 | -2.7 | -2.5 | -3.4 | -3.2 |
Nuclear Periphery | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 2.3 | 4.4 | 2.5 | 2.6 | – | 0 | 3.8 | 0 | 0 | -0.7 | 3.6 | 3.4 | 1.1 | 1.5 | 2.2 |
Peroxisomes | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 1.0 | 0 | 0 | 0 | – | 0 | 1.2 | 0 | 0 | 0 | 0 | 1.6 | 0 | 1.7 | 1.5 |
Vacuole | -0.1 | 0.7 | -0.1 | -1.1 | – | -4.2 | -2.6 | -2.4 | -3.4 | -1.1 | -1.0 | -4.0 | 3.6 | 3.3 | 3.8 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 0.2134 | 3.1189 | 3.5077 | 3.1182 | 1.3707 | 0.7584 | 0.8542 | 4.3337 | 4.702 | 4.2125 | 2.5589 | 1.8665 | -0.9046 | 2.4349 | 2.6701 | 1.6178 | 1.0084 | 0.2998 |
Actin | 0.0344 | 0.0037 | 0.0112 | 0.0001 | 0.0119 | 0.0061 | 0.0293 | 0.0002 | 0.0095 | 0.0003 | 0.004 | 0.0013 | 0.0295 | 0.0001 | 0.0181 | 0.0004 | 0.0042 | 0.0022 |
Bud | 0.005 | 0.0067 | 0.0021 | 0.0001 | 0.0009 | 0.0023 | 0.0065 | 0.0016 | 0.0008 | 0.0001 | 0.0002 | 0.0006 | 0.0103 | 0.0061 | 0.0006 | 0.0002 | 0.0002 | 0.0004 |
Bud Neck | 0.0061 | 0.0049 | 0.0011 | 0.0003 | 0.0017 | 0.0056 | 0.007 | 0.0007 | 0.001 | 0.0006 | 0.0012 | 0.0057 | 0.0073 | 0.0005 | 0.0013 | 0.0002 | 0.0006 | 0.0055 |
Bud Periphery | 0.0054 | 0.0051 | 0.0014 | 0.0001 | 0.001 | 0.0029 | 0.0073 | 0.0011 | 0.0011 | 0.0004 | 0.0004 | 0.0009 | 0.0114 | 0.0027 | 0.0006 | 0.0005 | 0.0003 | 0.0006 |
Bud Site | 0.0104 | 0.0202 | 0.0015 | 0.0001 | 0.009 | 0.0005 | 0.0134 | 0.0022 | 0.0015 | 0.0005 | 0.0004 | 0.0004 | 0.0138 | 0.0058 | 0.0053 | 0.001 | 0.0011 | 0.0005 |
Cell Periphery | 0.001 | 0.0003 | 0.0001 | 0 | 0.0002 | 0.0002 | 0.0012 | 0.0001 | 0.0002 | 0.0005 | 0.0001 | 0.0001 | 0.001 | 0.0002 | 0.0002 | 0.0002 | 0.0001 | 0.0001 |
Cytoplasm | 0.0488 | 0.0151 | 0.0008 | 0.0001 | 0.0209 | 0.0408 | 0.0278 | 0.0254 | 0.001 | 0.009 | 0.0293 | 0.0416 | 0.0641 | 0.0267 | 0.0007 | 0.0005 | 0.0516 | 0.0431 |
Cytoplasmic Foci | 0.014 | 0.0031 | 0.0008 | 0 | 0.0075 | 0.0158 | 0.0207 | 0.0011 | 0.0032 | 0.0032 | 0.0037 | 0.0026 | 0.0179 | 0.0012 | 0.0066 | 0.0077 | 0.0171 | 0.0016 |
Eisosomes | 0.0004 | 0 | 0.0001 | 0 | 0.0001 | 0 | 0.0005 | 0 | 0.0001 | 0 | 0 | 0 | 0.0003 | 0 | 0.0001 | 0 | 0 | 0 |
Endoplasmic Reticulum | 0.012 | 0.0009 | 0.0001 | 0 | 0.0037 | 0.001 | 0.0024 | 0.0007 | 0.0014 | 0.0023 | 0.001 | 0.0016 | 0.0043 | 0.002 | 0.0005 | 0.0009 | 0.0121 | 0.0014 |
Endosome | 0.0248 | 0.0013 | 0.001 | 0 | 0.0069 | 0.0023 | 0.0104 | 0.001 | 0.0026 | 0.011 | 0.0269 | 0.0024 | 0.0194 | 0.0004 | 0.0062 | 0.0056 | 0.0115 | 0.0046 |
Golgi | 0.008 | 0.0003 | 0.0004 | 0 | 0.0023 | 0.0019 | 0.0042 | 0 | 0.0014 | 0.0003 | 0.009 | 0.0004 | 0.0067 | 0 | 0.0049 | 0.0014 | 0.0037 | 0.0006 |
Lipid Particles | 0.0101 | 0.0003 | 0.0006 | 0 | 0.0023 | 0.004 | 0.0049 | 0.0001 | 0.0066 | 0.0017 | 0.0055 | 0.0017 | 0.0102 | 0.0001 | 0.0106 | 0.0271 | 0.0144 | 0.0014 |
Mitochondria | 0.0078 | 0.0009 | 0.0086 | 0.0004 | 0.001 | 0.0004 | 0.0028 | 0.0004 | 0.0123 | 0.0004 | 0.0036 | 0.0009 | 0.0058 | 0.0003 | 0.0095 | 0.0086 | 0.0018 | 0.0005 |
None | 0.0228 | 0.0168 | 0.0012 | 0.0001 | 0.0523 | 0.0148 | 0.0759 | 0.029 | 0.0011 | 0.0051 | 0.0073 | 0.0259 | 0.0602 | 0.031 | 0.0003 | 0.0006 | 0.006 | 0.027 |
Nuclear Periphery | 0.0481 | 0.0095 | 0.0049 | 0.0028 | 0.0198 | 0.0092 | 0.0087 | 0.0104 | 0.0101 | 0.0123 | 0.0068 | 0.011 | 0.02 | 0.0071 | 0.0049 | 0.0072 | 0.0201 | 0.0208 |
Nucleolus | 0.1104 | 0.0586 | 0.079 | 0.0605 | 0.0183 | 0.0907 | 0.1149 | 0.0429 | 0.088 | 0.0888 | 0.0509 | 0.0605 | 0.085 | 0.0258 | 0.0688 | 0.0444 | 0.0374 | 0.0426 |
Nucleus | 0.5079 | 0.7884 | 0.8392 | 0.8767 | 0.7098 | 0.6521 | 0.5669 | 0.8447 | 0.8323 | 0.8319 | 0.7707 | 0.7308 | 0.5392 | 0.8598 | 0.837 | 0.8777 | 0.6516 | 0.7087 |
Peroxisomes | 0.004 | 0.0001 | 0.0003 | 0 | 0.0012 | 0.0076 | 0.0035 | 0.0001 | 0.0004 | 0.0001 | 0.0015 | 0.0014 | 0.0101 | 0.0001 | 0.005 | 0.0019 | 0.0682 | 0.0002 |
Punctate Nuclear | 0.1033 | 0.0607 | 0.0451 | 0.0587 | 0.1264 | 0.1394 | 0.0817 | 0.0368 | 0.0237 | 0.0283 | 0.0656 | 0.1086 | 0.0718 | 0.0287 | 0.0175 | 0.0063 | 0.0949 | 0.1367 |
Vacuole | 0.0108 | 0.0026 | 0.0005 | 0.0001 | 0.0022 | 0.002 | 0.0081 | 0.0012 | 0.0009 | 0.0027 | 0.0071 | 0.0013 | 0.0094 | 0.0012 | 0.0009 | 0.0041 | 0.0025 | 0.001 |
Vacuole Periphery | 0.0044 | 0.0005 | 0.0002 | 0.0001 | 0.0007 | 0.0003 | 0.0019 | 0.0002 | 0.0007 | 0.0004 | 0.0049 | 0.0004 | 0.0024 | 0.0002 | 0.0005 | 0.0036 | 0.0007 | 0.0005 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 48.0023 | 7.5384 | 4.7333 | 19.1152 | 44.9957 | 51.8657 | 13.6319 | 8.5081 | 11.9808 | 50.6615 |
Translational Efficiency | 2.2815 | 2.0924 | 1.3791 | 1.4688 | 1.7298 | 2.2909 | 1.2535 | 1.1499 | 1.3207 | 1.491 |
Dataset | Hit |
---|---|
Protein Concentration | ✔ |
Protein Localization | ✘ |
Gene Expression | ✔ |
Translational Efficiency | ✔ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
86 | 1466 | 1880 | 683 | 1367 | 2241 | 401 | 1579 | 1453 | 3707 | 2281 | 2262 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 697.61 | 840.71 | 1109.88 | 1154.26 | 767.85 | 785.17 | 1103.43 | 1079.39 | 763.69 | 807.13 | 1108.75 | 1102.00 |
Standard Deviation | 147.54 | 187.75 | 212.75 | 221.32 | 133.27 | 147.12 | 170.35 | 211.17 | 135.18 | 166.62 | 205.94 | 217.02 |
Intensity Change Log 2 | — | 0.269188 | 0.669911 | 0.726476 | — | 0.032181 | 0.523099 | 0.491320 | — | 0.149867 | 0.594852 | 0.608050 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.000795 | 0.001335 | 0.000565 | 0.002230 | 0.000731 | 0.000760 | 0.001513 | 0.001162 | 0.000735 | 0.000987 | 0.000732 | 0.001485 |
Bud Neck | 0.004177 | 0.023704 | 0.036190 | 0.036204 | 0.011733 | 0.031154 | 0.014665 | 0.030627 | 0.011286 | 0.028208 | 0.032406 | 0.032311 |
Bud Site | 0.002365 | 0.010945 | 0.006062 | 0.021010 | 0.006707 | 0.010963 | 0.009291 | 0.010760 | 0.006450 | 0.010956 | 0.006630 | 0.013855 |
Cell Periphery | 0.000582 | 0.000853 | 0.000248 | 0.000288 | 0.000732 | 0.000654 | 0.000180 | 0.000266 | 0.000723 | 0.000733 | 0.000236 | 0.000273 |
Cytoplasm | 0.065911 | 0.102288* | 0.051375 | 0.016144 | 0.162507* | 0.100632* | 0.036281 | 0.032811 | 0.156790* | 0.101287* | 0.048721 | 0.027779 |
Cytoplasmic Foci | 0.011856 | 0.025380 | 0.000567 | 0.002749 | 0.029829 | 0.012336 | 0.002294 | 0.002343 | 0.028765 | 0.017495 | 0.000871 | 0.002465 |
Eisosomes | 0.000065 | 0.000079 | 0.000023 | 0.000044 | 0.000066 | 0.000051 | 0.000069 | 0.000037 | 0.000066 | 0.000062 | 0.000031 | 0.000039 |
Endoplasmic Reticulum | 0.002702 | 0.003938 | 0.001612 | 0.003388 | 0.003021 | 0.002967 | 0.005053 | 0.002606 | 0.003003 | 0.003351 | 0.002217 | 0.002842 |
Endosome | 0.002082 | 0.004626 | 0.000521 | 0.004913 | 0.003428 | 0.004114 | 0.001155 | 0.003993 | 0.003348 | 0.004317 | 0.000633 | 0.004270 |
Golgi | 0.000866 | 0.001457 | 0.000268 | 0.006386 | 0.001363 | 0.001735 | 0.000251 | 0.003058 | 0.001333 | 0.001625 | 0.000265 | 0.004063 |
Lipid Particles | 0.002824 | 0.003853 | 0.000135 | 0.000325 | 0.003629 | 0.001554 | 0.001023 | 0.000273 | 0.003581 | 0.002463 | 0.000291 | 0.000288 |
Mitochondria | 0.001744 | 0.008657 | 0.001836 | 0.005733 | 0.001943 | 0.006303 | 0.001643 | 0.005709 | 0.001931 | 0.007234 | 0.001802 | 0.005716 |
Mitotic Spindle | 0.075714 | 0.018996 | 0.014476 | 0.126839* | 0.012804 | 0.027654 | 0.011255 | 0.057532 | 0.016527 | 0.024230 | 0.013910 | 0.078459 |
None | 0.025678 | 0.025142 | 0.008149 | 0.005184 | 0.031827 | 0.022496 | 0.006049 | 0.011286 | 0.031463 | 0.023543 | 0.007780 | 0.009444 |
Nuclear Periphery | 0.001432 | 0.001135 | 0.001246 | 0.001826 | 0.000902 | 0.001354 | 0.000975 | 0.001592 | 0.000933 | 0.001268 | 0.001199 | 0.001663 |
Nuclear Periphery Foci | 0.008253 | 0.004896 | 0.000398 | 0.000741 | 0.006626 | 0.003426 | 0.000794 | 0.000639 | 0.006723 | 0.004007 | 0.000468 | 0.000670 |
Nucleolus | 0.299642* | 0.137002 | 0.047562 | 0.096786 | 0.144925 | 0.107675 | 0.043121 | 0.048772 | 0.154083 | 0.119273 | 0.046781 | 0.063270 |
Nucleus | 0.487383* | 0.612297* | 0.823518* | 0.653887* | 0.568466* | 0.655864* | 0.839659* | 0.773867* | 0.563667* | 0.638635* | 0.826356* | 0.737640* |
Peroxisomes | 0.004256 | 0.004210 | 0.000685 | 0.004806 | 0.002986 | 0.002632 | 0.002979 | 0.001549 | 0.003062 | 0.003256 | 0.001088 | 0.002533 |
Vacuole | 0.001172 | 0.007968 | 0.004214 | 0.007705 | 0.005061 | 0.004254 | 0.021498 | 0.007735 | 0.004831 | 0.005723 | 0.007253 | 0.007726 |
Vacuole Periphery | 0.000501 | 0.001239 | 0.000348 | 0.002810 | 0.000712 | 0.001420 | 0.000253 | 0.003381 | 0.000700 | 0.001349 | 0.000331 | 0.003208 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | -3.43 | 0.03 | -5.67 | -3.45 | -6.65 | -0.69 | -7.14 | -10.45 | -10.22 | 3.49 | -4.72 | -5.38 | -10.98 | -6.92 | -7.43 |
Bud Neck | -13.16 | -24.90 | -13.09 | -5.91 | 2.09 | -12.73 | -5.56 | -15.04 | -3.93 | -5.94 | -14.14 | -21.02 | -17.87 | -6.99 | 2.13 |
Bud Site | -8.18 | -7.78 | -5.48 | -1.06 | -2.51 | -4.03 | -1.01 | -1.82 | 1.42 | 0.10 | -5.21 | -2.29 | -3.46 | 0.45 | -1.76 |
Cell Periphery | -1.06 | 1.39 | 1.34 | 9.15 | -0.39 | 1.27 | 9.19 | 8.94 | 9.78 | -0.57 | -0.04 | 8.74 | 8.88 | 13.51 | 0.76 |
Cytoplasm | -2.25 | 1.07 | 2.44 | 14.07 | 6.76 | 7.60 | 16.43 | 18.03 | 14.54 | -0.01 | 7.44 | 16.81 | 18.87 | 19.65 | 5.10 |
Cytoplasmic Foci | -2.78 | 2.33 | 2.22 | 11.17 | -1.33 | 6.75 | 12.25 | 12.62 | 9.50 | 1.04 | 4.55 | 13.01 | 12.82 | 14.58 | -0.90 |
Eisosomes | -1.32 | 5.68 | 2.38 | 4.48 | -8.32 | 5.97 | -2.57 | 9.87 | 4.82 | 7.47 | 1.65 | 10.25 | 9.25 | 6.64 | -1.77 |
Endoplasmic Reticulum | -3.04 | 1.89 | -3.34 | -1.19 | -6.11 | 0.37 | -6.83 | -1.41 | -1.78 | 6.14 | -1.81 | 0.94 | -3.09 | -1.66 | -4.22 |
Endosome | -3.61 | 3.06 | -0.19 | 2.84 | -2.50 | -1.46 | 5.95 | 2.91 | 3.81 | -0.96 | -2.30 | 9.42 | 2.97 | 4.84 | -3.43 |
Golgi | -1.99 | 3.73 | -1.05 | -0.66 | -1.64 | -0.51 | 3.22 | 0.05 | 0.44 | -1.93 | -0.58 | 3.43 | -0.47 | -0.11 | -2.55 |
Lipid Particles | -1.03 | 2.12 | 1.76 | 5.59 | -3.62 | 4.00 | 4.87 | 7.24 | 7.56 | 4.13 | 1.87 | 7.33 | 7.26 | 8.30 | -0.43 |
Mitochondria | -3.94 | -1.94 | -5.38 | 1.64 | -4.97 | -4.33 | -0.26 | -4.41 | 1.41 | -4.84 | -5.58 | -0.71 | -5.71 | 2.23 | -6.54 |
Mitotic Spindle | 2.84 | 2.94 | -1.54 | -9.06 | -9.35 | -4.33 | -0.96 | -9.50 | -6.52 | -6.55 | -2.49 | -0.14 | -11.96 | -10.92 | -12.32 |
None | 0.06 | 2.48 | 2.74 | 12.35 | 3.80 | 4.00 | 12.09 | 9.89 | 8.89 | -4.64 | 3.70 | 11.92 | 10.96 | 13.74 | -2.38 |
Nuclear Periphery | 0.87 | -0.26 | -4.58 | -7.34 | -6.48 | -3.24 | -3.79 | -6.55 | -3.36 | -3.53 | -3.01 | -4.82 | -8.95 | -6.73 | -6.55 |
Nuclear Periphery Foci | 1.34 | 3.50 | 3.35 | 6.49 | -2.40 | 3.15 | 5.51 | 6.17 | 4.95 | 3.73 | 2.85 | 6.52 | 6.50 | 7.96 | -0.30 |
Nucleolus | 6.28 | 8.29 | 7.44 | 4.08 | -2.32 | 5.80 | 8.32 | 13.33 | 9.62 | 1.04 | 6.03 | 11.80 | 13.19 | 10.73 | 1.56 |
Nucleus | -4.22 | -9.69 | -5.20 | -2.59 | 9.16 | -8.88 | -15.11 | -17.79 | -10.55 | 2.37 | -8.54 | -23.81 | -16.36 | -10.70 | 6.75 |
Peroxisomes | -0.08 | 3.80 | 1.67 | 2.64 | -3.67 | 0.91 | -0.85 | 5.52 | 3.86 | 4.30 | -0.59 | 4.35 | 4.28 | 5.26 | 0.17 |
Vacuole | -7.05 | -5.73 | -6.67 | -5.16 | -5.68 | 1.43 | -4.72 | -4.79 | -5.37 | 3.04 | -1.30 | -4.14 | -7.55 | -7.13 | -4.32 |
Vacuole Periphery | -5.26 | 1.11 | -1.77 | -0.17 | -1.99 | -2.35 | 6.23 | -1.27 | -0.23 | -1.81 | -3.39 | 5.59 | -1.61 | -0.31 | -2.23 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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