Standard name | |
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Human Ortholog | |
Description | Essential alpha-kleisin subunit of the cohesin complex; required for sister chromatid cohesion in mitosis; subject to proteolytic cleavage by separate Esp1p, resulting in dissociation of cohesin from chromatin and the separation of sister chromatids at the mitotic metaphase-to-anaphase transition; apoptosis induces cleavage and translocation of a C-terminal fragment to mitochondria; expression peaks in S phase |
ensLOC | DeepLoc | |||||||||||||||||||||||
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Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 1 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 2 | 5 | – | 6 | 2 | 5 | 5 | 0 | 0 | 0 | 0 | 0 | 0 | 33 | 23 | 25 | 1 | 3 | 1 |
Bud Neck | 0 | 0 | 2 | 0 | 0 | 0 | 3 | – | 7 | 7 | 4 | 6 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 1 | 0 |
Bud Site | 1 | 1 | 0 | 0 | 0 | 0 | 3 | – | 3 | 4 | 6 | 8 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 97 | 48 | 80 | 91 | 77 | 126 | 173 | – | 127 | 120 | 126 | 153 | 71 | 54 | 83 | 50 | 50 | 56 | 56 | 23 | 42 | 23 | 68 | 108 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 12 | 12 | 7 | 1 | 0 | 0 | 0 | 0 | 0 |
Endosome | 9 | 3 | 5 | 5 | 5 | 13 | 9 | – | 1 | 5 | 3 | 4 | 3 | 5 | 6 | 47 | 41 | 34 | 5 | 1 | 1 | 0 | 4 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3 | 1 | 4 | 0 | 1 | 0 | 1 | 0 |
Mitochondria | 20 | 1 | 0 | 11 | 4 | 29 | 63 | – | 72 | 74 | 146 | 144 | 0 | 0 | 0 | 9 | 7 | 5 | 6 | 0 | 2 | 0 | 1 | 2 |
Nucleus | 95 | 42 | 85 | 75 | 97 | 102 | 110 | – | 48 | 78 | 65 | 93 | 60 | 38 | 53 | 70 | 57 | 58 | 90 | 58 | 80 | 5 | 4 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 2 | 1 | – | 0 | 3 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 41 | 18 | 21 | 41 | 68 | 60 | 78 | – | 62 | 80 | 71 | 98 | 12 | 31 | 43 | 32 | 33 | 38 | 37 | 15 | 26 | 0 | 0 | 0 |
Peroxisomes | 2 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 5 | 1 | 4 | 8 | 1 | 0 | 7 | – | 2 | 13 | 3 | 12 | 2 | 1 | 9 | 4 | 11 | 10 | 33 | 15 | 25 | 0 | 3 | 3 |
Vac/Vac Membrane | 21 | 35 | 22 | 24 | 33 | 35 | 34 | – | 6 | 17 | 19 | 17 | 11 | 9 | 2 | 55 | 50 | 54 | 16 | 11 | 22 | 3 | 5 | 4 |
Unique Cell Count | 259 | 124 | 202 | 225 | 254 | 327 | 419 | 290 | 340 | 350 | 437 | 146 | 122 | 183 | 228 | 215 | 213 | 291 | 153 | 234 | 35 | 100 | 125 | |
Labelled Cell Count | 291 | 149 | 219 | 255 | 285 | 369 | 487 | 335 | 403 | 449 | 540 | 159 | 140 | 197 | 280 | 265 | 263 | 291 | 153 | 234 | 35 | 100 | 125 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 5.2 | 6.9 | 6.9 | 6.4 | 7.4 | 6.0 | 6.1 | – | 4.9 | 5.4 | 4.4 | 4.9 | 7.6 | 8.2 | 8.1 | 9.4 | 9.5 | 9.4 | – | 5.4 | 5.5 |
Std Deviation (1e-4) | 1.9 | 2.8 | 2.1 | 1.9 | 2.1 | 1.8 | 2.4 | – | 1.9 | 2.1 | 1.6 | 1.8 | 2.8 | 3.0 | 3.0 | 3.9 | 4.0 | 3.6 | – | 1.7 | 1.2 |
Intensity Change (Log2) | – | – | – | -0.11 | 0.09 | -0.21 | -0.19 | – | -0.51 | -0.36 | -0.67 | -0.51 | 0.13 | 0.24 | 0.23 | 0.44 | 0.46 | 0.43 | – | -0.37 | -0.34 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 0.2 | -2.1 | -0.2 | 0.4 | – | 0.9 | -1.0 | -0.8 | -1.1 | 1.7 | 0.8 | 1.1 | -4.0 | -3.6 | -2.9 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3.3 | 3.4 | 0 |
Endosome | 0 | 0 | 0.9 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0.5 | 5.8 | 5.4 | 4.7 |
Golgi | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 3.2 | 0 | 4.4 | 5.8 | – | 0 | 7.1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | -1.9 | -0.8 | -2.5 | -4.0 | – | -6.3 | -4.7 | -6.0 | -5.5 | -0.2 | -2.0 | -2.7 | -2.5 | -3.4 | -3.2 |
Nuclear Periphery | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 2.3 | 4.4 | 2.5 | 2.6 | – | 0 | 3.8 | 0 | 0 | -0.7 | 3.6 | 3.4 | 1.1 | 1.5 | 2.2 |
Peroxisomes | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 1.0 | 0 | 0 | 0 | – | 0 | 1.2 | 0 | 0 | 0 | 0 | 1.6 | 0 | 1.7 | 1.5 |
Vacuole | -0.1 | 0.7 | -0.1 | -1.1 | – | -4.2 | -2.6 | -2.4 | -3.4 | -1.1 | -1.0 | -4.0 | 3.6 | 3.3 | 3.8 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 0.2134 | 3.1189 | 3.5077 | 3.1182 | 1.3707 | 0.7584 | 0.8542 | 4.3337 | 4.702 | 4.2125 | 2.5589 | 1.8665 | -0.9046 | 2.4349 | 2.6701 | 1.6178 | 1.0084 | 0.2998 |
Actin | 0.0344 | 0.0037 | 0.0112 | 0.0001 | 0.0119 | 0.0061 | 0.0293 | 0.0002 | 0.0095 | 0.0003 | 0.004 | 0.0013 | 0.0295 | 0.0001 | 0.0181 | 0.0004 | 0.0042 | 0.0022 |
Bud | 0.005 | 0.0067 | 0.0021 | 0.0001 | 0.0009 | 0.0023 | 0.0065 | 0.0016 | 0.0008 | 0.0001 | 0.0002 | 0.0006 | 0.0103 | 0.0061 | 0.0006 | 0.0002 | 0.0002 | 0.0004 |
Bud Neck | 0.0061 | 0.0049 | 0.0011 | 0.0003 | 0.0017 | 0.0056 | 0.007 | 0.0007 | 0.001 | 0.0006 | 0.0012 | 0.0057 | 0.0073 | 0.0005 | 0.0013 | 0.0002 | 0.0006 | 0.0055 |
Bud Periphery | 0.0054 | 0.0051 | 0.0014 | 0.0001 | 0.001 | 0.0029 | 0.0073 | 0.0011 | 0.0011 | 0.0004 | 0.0004 | 0.0009 | 0.0114 | 0.0027 | 0.0006 | 0.0005 | 0.0003 | 0.0006 |
Bud Site | 0.0104 | 0.0202 | 0.0015 | 0.0001 | 0.009 | 0.0005 | 0.0134 | 0.0022 | 0.0015 | 0.0005 | 0.0004 | 0.0004 | 0.0138 | 0.0058 | 0.0053 | 0.001 | 0.0011 | 0.0005 |
Cell Periphery | 0.001 | 0.0003 | 0.0001 | 0 | 0.0002 | 0.0002 | 0.0012 | 0.0001 | 0.0002 | 0.0005 | 0.0001 | 0.0001 | 0.001 | 0.0002 | 0.0002 | 0.0002 | 0.0001 | 0.0001 |
Cytoplasm | 0.0488 | 0.0151 | 0.0008 | 0.0001 | 0.0209 | 0.0408 | 0.0278 | 0.0254 | 0.001 | 0.009 | 0.0293 | 0.0416 | 0.0641 | 0.0267 | 0.0007 | 0.0005 | 0.0516 | 0.0431 |
Cytoplasmic Foci | 0.014 | 0.0031 | 0.0008 | 0 | 0.0075 | 0.0158 | 0.0207 | 0.0011 | 0.0032 | 0.0032 | 0.0037 | 0.0026 | 0.0179 | 0.0012 | 0.0066 | 0.0077 | 0.0171 | 0.0016 |
Eisosomes | 0.0004 | 0 | 0.0001 | 0 | 0.0001 | 0 | 0.0005 | 0 | 0.0001 | 0 | 0 | 0 | 0.0003 | 0 | 0.0001 | 0 | 0 | 0 |
Endoplasmic Reticulum | 0.012 | 0.0009 | 0.0001 | 0 | 0.0037 | 0.001 | 0.0024 | 0.0007 | 0.0014 | 0.0023 | 0.001 | 0.0016 | 0.0043 | 0.002 | 0.0005 | 0.0009 | 0.0121 | 0.0014 |
Endosome | 0.0248 | 0.0013 | 0.001 | 0 | 0.0069 | 0.0023 | 0.0104 | 0.001 | 0.0026 | 0.011 | 0.0269 | 0.0024 | 0.0194 | 0.0004 | 0.0062 | 0.0056 | 0.0115 | 0.0046 |
Golgi | 0.008 | 0.0003 | 0.0004 | 0 | 0.0023 | 0.0019 | 0.0042 | 0 | 0.0014 | 0.0003 | 0.009 | 0.0004 | 0.0067 | 0 | 0.0049 | 0.0014 | 0.0037 | 0.0006 |
Lipid Particles | 0.0101 | 0.0003 | 0.0006 | 0 | 0.0023 | 0.004 | 0.0049 | 0.0001 | 0.0066 | 0.0017 | 0.0055 | 0.0017 | 0.0102 | 0.0001 | 0.0106 | 0.0271 | 0.0144 | 0.0014 |
Mitochondria | 0.0078 | 0.0009 | 0.0086 | 0.0004 | 0.001 | 0.0004 | 0.0028 | 0.0004 | 0.0123 | 0.0004 | 0.0036 | 0.0009 | 0.0058 | 0.0003 | 0.0095 | 0.0086 | 0.0018 | 0.0005 |
None | 0.0228 | 0.0168 | 0.0012 | 0.0001 | 0.0523 | 0.0148 | 0.0759 | 0.029 | 0.0011 | 0.0051 | 0.0073 | 0.0259 | 0.0602 | 0.031 | 0.0003 | 0.0006 | 0.006 | 0.027 |
Nuclear Periphery | 0.0481 | 0.0095 | 0.0049 | 0.0028 | 0.0198 | 0.0092 | 0.0087 | 0.0104 | 0.0101 | 0.0123 | 0.0068 | 0.011 | 0.02 | 0.0071 | 0.0049 | 0.0072 | 0.0201 | 0.0208 |
Nucleolus | 0.1104 | 0.0586 | 0.079 | 0.0605 | 0.0183 | 0.0907 | 0.1149 | 0.0429 | 0.088 | 0.0888 | 0.0509 | 0.0605 | 0.085 | 0.0258 | 0.0688 | 0.0444 | 0.0374 | 0.0426 |
Nucleus | 0.5079 | 0.7884 | 0.8392 | 0.8767 | 0.7098 | 0.6521 | 0.5669 | 0.8447 | 0.8323 | 0.8319 | 0.7707 | 0.7308 | 0.5392 | 0.8598 | 0.837 | 0.8777 | 0.6516 | 0.7087 |
Peroxisomes | 0.004 | 0.0001 | 0.0003 | 0 | 0.0012 | 0.0076 | 0.0035 | 0.0001 | 0.0004 | 0.0001 | 0.0015 | 0.0014 | 0.0101 | 0.0001 | 0.005 | 0.0019 | 0.0682 | 0.0002 |
Punctate Nuclear | 0.1033 | 0.0607 | 0.0451 | 0.0587 | 0.1264 | 0.1394 | 0.0817 | 0.0368 | 0.0237 | 0.0283 | 0.0656 | 0.1086 | 0.0718 | 0.0287 | 0.0175 | 0.0063 | 0.0949 | 0.1367 |
Vacuole | 0.0108 | 0.0026 | 0.0005 | 0.0001 | 0.0022 | 0.002 | 0.0081 | 0.0012 | 0.0009 | 0.0027 | 0.0071 | 0.0013 | 0.0094 | 0.0012 | 0.0009 | 0.0041 | 0.0025 | 0.001 |
Vacuole Periphery | 0.0044 | 0.0005 | 0.0002 | 0.0001 | 0.0007 | 0.0003 | 0.0019 | 0.0002 | 0.0007 | 0.0004 | 0.0049 | 0.0004 | 0.0024 | 0.0002 | 0.0005 | 0.0036 | 0.0007 | 0.0005 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 48.0023 | 7.5384 | 4.7333 | 19.1152 | 44.9957 | 51.8657 | 13.6319 | 8.5081 | 11.9808 | 50.6615 |
Translational Efficiency | 2.2815 | 2.0924 | 1.3791 | 1.4688 | 1.7298 | 2.2909 | 1.2535 | 1.1499 | 1.3207 | 1.491 |
Dataset | Hit |
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Protein Concentration | ✔ |
Protein Localization | ✘ |
Gene Expression | ✔ |
Translational Efficiency | ✔ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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