ORF | |
---|---|
Human Ortholog | |
Description | Tail-anchored plasma membrane protein with a conserved CYSTM module; possibly involved in response to stress; may contribute to non-homologous end-joining (NHEJ) based on ydl012c htz1 double null phenotype; YDL012C has a paralog, YBR016W, that arose from the whole genome duplication |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 121 | 82 | 50 | 33 | 94 | 57 | – | 49 | 32 | 70 | 42 | 55 | 44 | 77 | 52 | 32 | 35 | 37 | 31 | 28 | 11 | 11 | 22 | 19 |
Bud | 33 | 12 | 23 | 16 | 21 | 22 | – | 51 | 26 | 27 | 24 | 26 | 9 | 11 | 6 | 4 | 5 | 6 | 8 | 4 | 3 | 0 | 3 | 4 |
Bud Neck | 10 | 11 | 5 | 0 | 3 | 0 | – | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 60 | 44 | 22 | 11 | 26 | 27 |
Bud Site | 10 | 7 | 4 | 3 | 6 | 2 | – | 5 | 1 | 2 | 2 | 1 | 0 | 1 | 1 | 1 | 0 | 1 | – | – | – | – | – | – |
Cell Periphery | 203 | 169 | 102 | 161 | 158 | 164 | – | 176 | 151 | 174 | 180 | 181 | 145 | 212 | 262 | 74 | 89 | 98 | 381 | 287 | 194 | 88 | 195 | 184 |
Cytoplasm | 162 | 180 | 116 | 80 | 121 | 165 | – | 296 | 173 | 174 | 141 | 142 | 111 | 161 | 178 | 19 | 24 | 24 | 29 | 25 | 15 | 6 | 17 | 15 |
Endoplasmic Reticulum | 2 | 10 | 3 | 1 | 11 | 14 | – | 5 | 8 | 8 | 6 | 6 | 1 | 1 | 5 | 0 | 5 | 6 | 9 | 12 | 9 | 4 | 13 | 10 |
Endosome | 1 | 0 | 0 | 2 | 4 | 2 | – | 2 | 0 | 1 | 1 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 2 | 3 | 1 | 1 | 2 | 2 |
Golgi | 0 | 1 | 0 | 0 | 1 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 5 | 3 | 0 | 2 | 0 | 0 | 1 | 0 |
Mitochondria | 29 | 18 | 65 | 176 | 86 | 176 | – | 308 | 213 | 247 | 239 | 272 | 19 | 25 | 30 | 14 | 14 | 14 | 14 | 11 | 9 | 4 | 6 | 6 |
Nucleus | 3 | 1 | 1 | 0 | 1 | 1 | – | 2 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 2 | 4 | 0 | 0 | 0 | 1 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 1 | 0 | 0 | – | 3 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 1 | 0 | 1 | 0 | – | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 2 | 1 | 2 | 13 | 23 | 18 | – | 22 | 8 | 13 | 8 | 11 | 7 | 13 | 20 | 3 | 3 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 10 | 13 | 5 | 3 | 7 | 3 | – | 11 | 6 | 3 | 3 | 5 | 3 | 6 | 6 | 7 | 3 | 6 | 0 | 0 | 0 | 0 | 0 | 0 |
Vac/Vac Membrane | 0 | 0 | 1 | 1 | 2 | 5 | – | 21 | 14 | 1 | 4 | 6 | 0 | 1 | 3 | 1 | 0 | 4 | 19 | 13 | 1 | 8 | 3 | 5 |
Unique Cell Count | 407 | 356 | 240 | 287 | 356 | 402 | 663 | 414 | 482 | 403 | 446 | 223 | 337 | 375 | 120 | 141 | 143 | 562 | 440 | 276 | 143 | 297 | 283 | |
Labelled Cell Count | 586 | 505 | 378 | 490 | 539 | 629 | 953 | 633 | 722 | 652 | 705 | 339 | 508 | 565 | 159 | 186 | 203 | 562 | 440 | 276 | 143 | 297 | 283 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 6.0 | 6.1 | 5.0 | 5.6 | 6.0 | 5.2 | – | 5.2 | 4.9 | 5.1 | 5.1 | 5.1 | 7.1 | 7.0 | 6.9 | 11.1 | 10.8 | 10.3 | 6.3 | 7.3 | 7.1 |
Std Deviation (1e-4) | 0.7 | 0.7 | 1.6 | 1.7 | 1.3 | 1.9 | – | 1.3 | 1.1 | 0.9 | 1.3 | 1.2 | 1.5 | 1.3 | 1.4 | 2.0 | 2.1 | 1.9 | 1.5 | 1.8 | 1.8 |
Intensity Change (Log2) | – | – | – | 0.17 | 0.26 | 0.08 | – | 0.08 | -0.03 | 0.04 | 0.03 | 0.03 | 0.51 | 0.5 | 0.47 | 1.16 | 1.12 | 1.06 | 0.35 | 0.56 | 0.51 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | -2.9 | 1.6 | -2.2 | – | -5.7 | 0 | -2.1 | -3.6 | -2.9 | -0.3 | 0.6 | -2.3 | 1.2 | 0.9 | 1.1 |
Bud | -1.8 | -1.7 | -2.0 | – | -0.9 | 0 | -2.0 | -1.7 | -1.8 | -2.4 | -3.2 | -4.6 | -2.1 | -2.2 | -1.9 |
Bud Neck | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 3.1 | 0.5 | -0.4 | – | -4.6 | 0 | -1.7 | 0.5 | -0.5 | 4.9 | 4.9 | 6.7 | 3.4 | 3.9 | 4.9 |
Cytoplasm | -4.8 | -3.5 | -1.8 | – | -1.0 | -1.6 | -3.2 | -3.3 | -4.3 | 0.3 | -0.1 | -0.2 | -6.0 | -6.1 | -6.2 |
Endoplasmic Reticulum | 0 | 1.5 | 1.7 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 7.9 | -0.8 | 4.2 | – | 5.2 | 0 | 6.2 | 7.9 | 8.5 | -5.2 | -6.4 | -6.4 | -3.3 | -4.0 | -4.0 |
Nucleus | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 2.5 | 3.4 | 2.6 | – | 2.1 | 0 | 0 | 0 | 0 | 0 | 2.3 | 2.9 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | 0 | 0 | – | 2.4 | 2.4 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 3.9638 | 3.9584 | 3.6156 | 3.3888 | 2.995 | 3.7121 | 3.3494 | 3.6402 | 3.2561 | 2.8984 | 3.1781 | 3.3064 | 5.6603 | 5.1634 | 4.7356 | 4.6553 | 4.5173 | 5.0617 |
Actin | 0.0103 | 0.0015 | 0.0073 | 0.0514 | 0.0417 | 0.018 | 0.0626 | 0.0028 | 0.0162 | 0.0182 | 0.0066 | 0.0009 | 0.0368 | 0.0009 | 0.0131 | 0.0091 | 0.0041 | 0.0018 |
Bud | 0.0003 | 0.0005 | 0.0008 | 0.0009 | 0.0006 | 0.0005 | 0.0072 | 0.0009 | 0.0016 | 0.0008 | 0.0037 | 0.0017 | 0.0007 | 0.0007 | 0.0011 | 0.0009 | 0.0002 | 0.0005 |
Bud Neck | 0.0017 | 0.0055 | 0.0146 | 0.0101 | 0.01 | 0.062 | 0.0081 | 0.0082 | 0.0058 | 0.0092 | 0.0038 | 0.0489 | 0.0034 | 0.0066 | 0.006 | 0.0009 | 0.0004 | 0.0501 |
Bud Periphery | 0.0024 | 0.0028 | 0.0125 | 0.012 | 0.0084 | 0.0083 | 0.0152 | 0.0048 | 0.0141 | 0.0083 | 0.0139 | 0.0098 | 0.0046 | 0.005 | 0.015 | 0.0276 | 0.0027 | 0.0092 |
Bud Site | 0.0116 | 0.1437 | 0.0387 | 0.0075 | 0.0038 | 0.0026 | 0.0273 | 0.1678 | 0.04 | 0.0054 | 0.0193 | 0.0016 | 0.0112 | 0.1795 | 0.0361 | 0.0008 | 0.0005 | 0.002 |
Cell Periphery | 0.8156 | 0.7588 | 0.7832 | 0.7569 | 0.8705 | 0.8318 | 0.7043 | 0.6994 | 0.7977 | 0.8265 | 0.745 | 0.8721 | 0.8048 | 0.6487 | 0.7877 | 0.848 | 0.7735 | 0.8616 |
Cytoplasm | 0.0694 | 0.0808 | 0.0933 | 0.0779 | 0.045 | 0.0549 | 0.0531 | 0.0983 | 0.082 | 0.0603 | 0.0476 | 0.0517 | 0.0586 | 0.1404 | 0.0997 | 0.0599 | 0.0719 | 0.0533 |
Cytoplasmic Foci | 0.0125 | 0.0001 | 0.0054 | 0.0096 | 0.0018 | 0.0011 | 0.0107 | 0.0007 | 0.0019 | 0.0011 | 0.0245 | 0.0002 | 0.0104 | 0.0027 | 0.008 | 0.0124 | 0.0027 | 0.0003 |
Eisosomes | 0.0077 | 0.0021 | 0.0023 | 0.0015 | 0.0008 | 0.0011 | 0.0185 | 0.0024 | 0.0009 | 0.0025 | 0.0008 | 0.0006 | 0.0034 | 0.0008 | 0.0016 | 0.0003 | 0.0003 | 0.0007 |
Endoplasmic Reticulum | 0.0131 | 0.0011 | 0.0035 | 0.005 | 0.0057 | 0.0017 | 0.0139 | 0.0053 | 0.0073 | 0.0166 | 0.0168 | 0.0027 | 0.009 | 0.0013 | 0.0085 | 0.0039 | 0.0024 | 0.0023 |
Endosome | 0.0128 | 0 | 0.0068 | 0.0126 | 0.0018 | 0.0013 | 0.0117 | 0.0001 | 0.0027 | 0.0075 | 0.021 | 0.0003 | 0.0072 | 0.0004 | 0.001 | 0.0011 | 0.0122 | 0.0003 |
Golgi | 0.0049 | 0.0001 | 0.0018 | 0.0085 | 0.0037 | 0.0028 | 0.0124 | 0.0003 | 0.0032 | 0.0068 | 0.0062 | 0.0004 | 0.0051 | 0.0003 | 0.0011 | 0.0015 | 0.0024 | 0.0011 |
Lipid Particles | 0.0124 | 0.0001 | 0.0122 | 0.0133 | 0.0006 | 0.0026 | 0.0087 | 0.0042 | 0.0021 | 0.0038 | 0.0156 | 0.0003 | 0.0074 | 0.0013 | 0.0017 | 0.0025 | 0.017 | 0.0018 |
Mitochondria | 0.0021 | 0 | 0.0013 | 0.0062 | 0.0003 | 0.005 | 0.0149 | 0.0003 | 0.0046 | 0.0043 | 0.013 | 0.0045 | 0.0014 | 0 | 0.0006 | 0.0002 | 0.0432 | 0.0056 |
None | 0.0024 | 0.0002 | 0.004 | 0.0012 | 0.0006 | 0.0011 | 0.0035 | 0.0006 | 0.0072 | 0.0198 | 0.0202 | 0.0006 | 0.0169 | 0.0016 | 0.0019 | 0.0006 | 0.0044 | 0.0029 |
Nuclear Periphery | 0.0033 | 0.0002 | 0.0003 | 0.0004 | 0.0003 | 0.0002 | 0.0038 | 0.0004 | 0.0025 | 0.0016 | 0.0044 | 0.0001 | 0.0036 | 0.0003 | 0.0006 | 0.0004 | 0.0213 | 0.0001 |
Nucleolus | 0.0004 | 0 | 0.0002 | 0.0006 | 0 | 0.0001 | 0.0011 | 0.0001 | 0.0003 | 0.0004 | 0.0033 | 0.0001 | 0.0003 | 0.0001 | 0.0002 | 0.0001 | 0.0009 | 0.0001 |
Nucleus | 0.0004 | 0.0002 | 0.0002 | 0.0003 | 0.0001 | 0.0002 | 0.002 | 0.0003 | 0.0006 | 0.0009 | 0.0046 | 0.0002 | 0.0009 | 0.0002 | 0.0004 | 0.0001 | 0.0138 | 0.0002 |
Peroxisomes | 0.0101 | 0 | 0.007 | 0.0182 | 0.0002 | 0.0015 | 0.0056 | 0 | 0.0028 | 0.0001 | 0.0049 | 0 | 0.0052 | 0.0012 | 0.0008 | 0.0158 | 0.0069 | 0.0016 |
Punctate Nuclear | 0.0015 | 0 | 0.0003 | 0.0004 | 0.0001 | 0.0002 | 0.0007 | 0 | 0.0002 | 0.0003 | 0.0133 | 0 | 0.0043 | 0.0055 | 0.011 | 0.0089 | 0.0138 | 0 |
Vacuole | 0.0048 | 0.0023 | 0.0042 | 0.0048 | 0.0039 | 0.003 | 0.0133 | 0.003 | 0.0058 | 0.0049 | 0.0095 | 0.0033 | 0.0045 | 0.0026 | 0.0038 | 0.0049 | 0.0042 | 0.0041 |
Vacuole Periphery | 0.0005 | 0 | 0.0002 | 0.0008 | 0 | 0.0002 | 0.0013 | 0.0001 | 0.0003 | 0.0008 | 0.0019 | 0.0001 | 0.0003 | 0 | 0.0001 | 0.0001 | 0.0011 | 0.0001 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 30.6501 | 51.7543 | 47.4401 | 61.804 | 61.8166 | 44.2479 | 48.481 | 66.4816 | 76.997 | 54.1361 |
Translational Efficiency | 2.4934 | 2.5984 | 2.4031 | 1.7494 | 1.7149 | 2.0967 | 2.678 | 1.7545 | 1.3762 | 2.1904 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✔ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
1669 | 1673 | 338 | 457 | 834 | 1634 | 2588 | 1676 | 2503 | 3307 | 2926 | 2133 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 723.44 | 859.72 | 1078.77 | 1052.76 | 713.29 | 808.89 | 1046.78 | 1001.12 | 720.06 | 834.60 | 1050.48 | 1012.18 |
Standard Deviation | 104.69 | 111.60 | 115.38 | 131.22 | 94.90 | 112.06 | 115.05 | 120.88 | 101.64 | 114.68 | 115.55 | 124.98 |
Intensity Change Log 2 | — | 0.248993 | 0.576442 | 0.541231 | — | 0.181455 | 0.553398 | 0.489054 | — | 0.215858 | 0.565047 | 0.515563 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.006987 | 0.012622 | 0.035134 | 0.065609 | 0.005099 | 0.010124 | 0.018178 | 0.057067 | 0.006358 | 0.011388 | 0.020137 | 0.058897 |
Bud Neck | 0.103951 | 0.086780 | 0.060935 | 0.076224 | 0.047472 | 0.088029 | 0.107143 | 0.085103 | 0.085132 | 0.087398 | 0.101805 | 0.083201 |
Bud Site | 0.059920 | 0.087323 | 0.184053* | 0.220744* | 0.043836 | 0.086254 | 0.103807* | 0.210767* | 0.054561 | 0.086795 | 0.113076* | 0.212905* |
Cell Periphery | 0.105524* | 0.239366* | 0.181421* | 0.345328* | 0.349268* | 0.187248* | 0.404758* | 0.303720* | 0.186739* | 0.213614* | 0.378959* | 0.312634* |
Cytoplasm | 0.525907* | 0.499205* | 0.216872* | 0.160190* | 0.460526* | 0.512000* | 0.281262* | 0.255961* | 0.504122* | 0.505527* | 0.273824* | 0.235442* |
Cytoplasmic Foci | 0.054037 | 0.009756 | 0.024086 | 0.006315 | 0.010590 | 0.024592 | 0.001887 | 0.003709 | 0.039560 | 0.017086 | 0.004451 | 0.004267 |
Eisosomes | 0.027854 | 0.022034 | 0.022492 | 0.040328 | 0.022768 | 0.026345 | 0.024700 | 0.028685 | 0.026159 | 0.024164 | 0.024445 | 0.031180 |
Endoplasmic Reticulum | 0.007411 | 0.004603 | 0.110033* | 0.017192 | 0.010836 | 0.004572 | 0.013601 | 0.005508 | 0.008552 | 0.004587 | 0.024741 | 0.008011 |
Endosome | 0.012696 | 0.002636 | 0.004110 | 0.001800 | 0.002654 | 0.005685 | 0.000765 | 0.002101 | 0.009350 | 0.004142 | 0.001152 | 0.002036 |
Golgi | 0.019283 | 0.005541 | 0.004341 | 0.002848 | 0.005491 | 0.011633 | 0.000850 | 0.001848 | 0.014688 | 0.008551 | 0.001253 | 0.002062 |
Lipid Particles | 0.025035 | 0.002715 | 0.009277 | 0.002714 | 0.003668 | 0.006542 | 0.002114 | 0.001489 | 0.017915 | 0.004606 | 0.002941 | 0.001751 |
Mitochondria | 0.008060 | 0.004053 | 0.000445 | 0.000449 | 0.007648 | 0.006719 | 0.000842 | 0.001648 | 0.007923 | 0.005370 | 0.000797 | 0.001391 |
Mitotic Spindle | 0.000081 | 0.000191 | 0.000230 | 0.000298 | 0.000095 | 0.000309 | 0.000321 | 0.001532 | 0.000086 | 0.000249 | 0.000311 | 0.001268 |
None | 0.000834 | 0.000124 | 0.000134 | 0.000048 | 0.001284 | 0.000351 | 0.000212 | 0.000100 | 0.000984 | 0.000237 | 0.000203 | 0.000089 |
Nuclear Periphery | 0.000141 | 0.000065 | 0.000221 | 0.000092 | 0.000142 | 0.000106 | 0.000240 | 0.000064 | 0.000141 | 0.000085 | 0.000238 | 0.000070 |
Nuclear Periphery Foci | 0.000334 | 0.000217 | 0.006517 | 0.000909 | 0.000329 | 0.000284 | 0.000601 | 0.000332 | 0.000332 | 0.000250 | 0.001285 | 0.000456 |
Nucleolus | 0.000286 | 0.000055 | 0.000053 | 0.000015 | 0.000937 | 0.000213 | 0.000060 | 0.000022 | 0.000503 | 0.000133 | 0.000059 | 0.000021 |
Nucleus | 0.009770 | 0.003495 | 0.037053 | 0.008087 | 0.007281 | 0.005065 | 0.010384 | 0.008128 | 0.008941 | 0.004271 | 0.013465 | 0.008119 |
Peroxisomes | 0.009721 | 0.006618 | 0.001275 | 0.001527 | 0.009632 | 0.008807 | 0.001345 | 0.001300 | 0.009691 | 0.007699 | 0.001337 | 0.001349 |
Vacuole | 0.020830 | 0.011642 | 0.101117 | 0.048902 | 0.009291 | 0.014225 | 0.026446 | 0.030500 | 0.016985 | 0.012918 | 0.035072 | 0.034443 |
Vacuole Periphery | 0.001338 | 0.000959 | 0.000201 | 0.000379 | 0.001152 | 0.000895 | 0.000483 | 0.000418 | 0.001276 | 0.000928 | 0.000450 | 0.000410 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | -6.28 | -9.54 | -10.20 | -9.16 | -4.75 | -5.44 | -12.19 | -20.62 | -18.20 | -14.72 | -7.79 | -14.29 | -22.65 | -20.16 | -15.71 |
Bud Neck | 3.13 | 6.47 | 3.68 | 1.49 | -1.85 | -8.43 | -12.56 | -7.57 | 0.63 | 4.45 | -0.59 | -3.86 | 0.50 | 1.10 | 4.23 |
Bud Site | -6.26 | -12.35 | -15.00 | -12.17 | -2.68 | -8.77 | -13.45 | -25.97* | -18.40 | -16.43 | -9.86 | -15.74 | -29.52 | -22.59 | -17.07 |
Cell Periphery | -17.86* | -6.61 | -16.80 | -7.04 | -9.68 | 13.19 | -4.57 | 3.60 | -13.02 | 11.69 | -4.04 | -26.36 | -15.55 | -13.22 | 8.43 |
Cytoplasm | 2.96 | 26.23 | 35.05 | 32.21 | 4.32 | -4.45 | 16.73 | 18.40 | 30.24 | 3.50 | -0.22 | 33.84 | 37.79 | 41.15 | 5.99 |
Cytoplasmic Foci | 15.79 | 8.37 | 14.02 | 1.40 | 5.70 | -6.33 | 6.63 | 4.97 | 11.34 | -2.89 | 10.68 | 18.47 | 17.99 | 10.80 | 0.28 |
Eisosomes | 3.49 | 2.47 | -3.72 | -5.60 | -5.00 | -1.87 | -1.18 | -3.07 | -1.28 | -2.58 | 1.55 | 1.38 | -3.21 | -4.76 | -4.68 |
Endoplasmic Reticulum | 2.87 | -12.34 | -4.52 | -5.88 | 10.88 | 3.80 | -1.57 | 3.29 | -1.07 | 7.21 | 4.70 | -10.38 | 0.55 | -4.31 | 10.87 |
Endosome | 10.26 | 8.54 | 8.98 | 1.03 | 2.81 | -5.20 | 7.14 | 1.01 | 4.78 | -2.40 | 7.40 | 12.60 | 9.34 | 3.90 | -1.86 |
Golgi | 10.83 | 12.87 | 9.45 | 1.83 | 1.06 | -4.84 | 6.38 | 4.45 | 8.66 | -2.30 | 6.14 | 16.85 | 14.12 | 8.70 | -1.79 |
Lipid Particles | 11.07 | 7.54 | 10.98 | -0.03 | 9.73 | -3.53 | 3.35 | 4.53 | 6.53 | 1.52 | 9.46 | 10.85 | 11.73 | 6.35 | 3.31 |
Mitochondria | 5.34 | 11.76 | 11.77 | 8.63 | -0.04 | 0.85 | 7.85 | 6.05 | 6.22 | -1.64 | 3.99 | 13.83 | 10.21 | 7.36 | -1.53 |
Mitotic Spindle | -1.98 | -10.25 | -3.03 | -1.24 | -1.02 | -2.48 | -6.50 | -2.19 | -1.84 | -1.85 | -3.26 | -10.97 | -2.30 | -1.96 | -1.86 |
None | 4.91 | 4.75 | 5.45 | 5.06 | 2.47 | 0.99 | 1.14 | 1.26 | 3.56 | 2.74 | 2.28 | 2.39 | 2.73 | 3.64 | 3.34 |
Nuclear Periphery | 3.58 | -3.28 | 2.15 | -2.89 | 8.17 | 0.79 | -4.67 | 4.23 | 1.00 | 16.98 | 2.16 | -5.50 | 4.61 | 0.72 | 17.93 |
Nuclear Periphery Foci | 4.04 | -12.68 | -7.10 | -8.90 | 11.37 | 0.48 | -3.08 | 0.03 | -0.79 | 7.81 | 1.98 | -12.22 | -3.11 | -5.92 | 11.11 |
Nucleolus | 2.92 | 2.95 | 3.41 | 15.04 | 12.28 | 1.21 | 1.49 | 1.54 | 1.62 | 14.60 | 1.68 | 2.11 | 2.27 | 1.89 | 16.84 |
Nucleus | 12.06 | -10.31 | 1.20 | -5.02 | 10.29 | 1.55 | -1.91 | -0.57 | -5.01 | 3.24 | 7.39 | -5.77 | 0.86 | -8.40 | 7.71 |
Peroxisomes | 4.33 | 16.06 | 12.71 | 7.74 | -0.58 | 0.77 | 9.54 | 9.31 | 10.04 | 0.19 | 3.21 | 18.77 | 17.27 | 13.19 | -0.06 |
Vacuole | 7.96 | -12.83 | -6.84 | -9.06 | 7.02 | -5.20 | -16.29 | -13.27 | -10.43 | -2.81 | 4.99 | -14.21 | -11.25 | -14.29 | 0.04 |
Vacuole Periphery | 1.46 | 9.99 | 4.58 | 1.96 | -0.97 | 1.28 | 2.09 | 3.38 | 3.57 | 0.23 | 2.22 | 3.32 | 6.45 | 3.30 | 0.17 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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