Standard name
Human Ortholog
Description Dual-specificity kinase; autophosphorylation required for function; required for spindle pole body (SPB) duplication and spindle checkpoint function; contributes to bi-orientation by promoting formation of force-generating kinetochore-microtubule attachments in meiosis I; substrates include SPB proteins Spc42p, Spc110p, and Spc98p, mitotic exit network protein Mob1p, kinetochore protein Cnn1p, and checkpoint protein Mad1p; substrate of APCC(Cdh1); similar to human Mps1p
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0.19 0.24 0.23 0.18 0.11 0.23 0.1 0 0 0 0 0 0.16 0 0 0.18 0.09 0.15 0.11 0.11 0.1 0.13 0.18 0.1
Bud 0 0.09 0.06 0.05 0.08 0.12 0.07 0.06 0.06 0.07 0.06 0 0.06 0 0 0 0 0.06 0.14 0.18 0.14 0.18 0.05 0
Bud Neck 0.05 0.08 0.11 0.09 0.1 0.08 0.09 0.06 0 0 0 0.05 0 0 0 0.05 0.06 0 0.33 0.33 0.37 0.27 0.1 0.05
Bud Site 0.1 0.17 0.18 0.09 0.1 0.22 0.05 0 0 0 0 0 0.11 0 0 0.11 0.05 0.11 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0.05 0.08 0.05
Cytoplasm 0.62 0.54 0.55 0.67 0.62 0.44 0.66 0.74 0.73 0.66 0.67 0.71 0.74 0.95 0.97 0.59 0.63 0.6 0.24 0.21 0.16 0.19 0.36 0.54
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0.05 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.1 0 0 0 0 0.08 0.12 0.1 0.25 0.34 0.4 0.2 0 0 0 0 0 0.06 0 0.05 0.08 0.07 0.13 0.09
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0.08 0 0 0 0 0.05 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0.09 0.24 0.12 0.18 0.2 0.08 0.07 0.06 0.05 0 0.05 0 0 0 0.15 0.15 0.14 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 14 51 75 47 30 71 35 10 5 7 3 9 36 5 1 40 14 19 11 27 35 25 40 24
Bud 3 19 20 14 22 35 24 22 26 23 16 5 13 5 1 7 5 7 14 46 52 37 12 9
Bud Neck 4 16 36 22 28 24 33 21 18 10 10 15 2 1 2 10 9 0 34 85 136 54 22 12
Bud Site 7 35 57 24 27 67 19 4 15 5 4 5 23 1 1 24 8 14 – – – – – –
Cell Periphery 1 8 6 10 1 7 6 4 3 2 1 2 1 4 4 4 8 1 1 7 14 9 16 13
Cytoplasm 45 113 175 174 172 132 233 275 294 206 174 198 162 247 207 130 95 75 24 53 59 38 81 138
Endoplasmic Reticulum 1 4 0 1 5 5 12 5 1 2 1 4 2 4 2 10 5 6 1 1 2 4 5 7
Endosome 1 6 1 0 0 0 0 1 1 0 0 0 0 0 0 4 6 4 3 4 8 5 2 2
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2 1 1 0 0 2 1 1 1
Mitochondria 7 5 0 2 6 24 42 37 102 108 104 56 3 2 2 9 5 8 3 13 28 13 29 24
Nucleus 0 2 5 4 8 6 2 7 1 2 0 2 1 0 0 4 2 6 0 0 1 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 1 0 0 1 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 1 0 0 1 3 1 6 1 2 1 1 0 1 0 0 1 1 0 0 0 0 0 1
Peroxisomes 6 8 3 4 0 14 0 0 3 0 1 4 12 3 1 3 4 4 0 0 1 0 0 1
SpindlePole 1 19 77 32 50 60 29 27 24 15 7 14 3 3 0 33 23 17 2 2 9 3 0 2
Vac/Vac Membrane 0 1 0 0 3 1 0 5 2 1 3 2 0 0 0 4 3 2 3 10 11 4 10 11
Unique Cell Count 73 209 321 258 276 303 355 371 402 314 261 277 219 261 214 220 151 125 104 257 368 201 225 257
Labelled Cell Count 90 288 455 334 353 449 436 424 496 384 325 317 259 276 221 284 189 165 104 257 368 201 225 257


Bud Neck

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.5 5.2 5.3 5.0 5.3 4.3 4.1 4.4 3.4 3.2 3.0 3.6 4.5 4.4 4.3 7.0 7.2 6.5 4.5 4.2 4.5
Std Deviation (1e-4) 0.9 1.0 1.2 1.4 1.7 1.2 0.9 1.1 0.7 1.0 0.7 0.7 1.0 1.4 1.6 1.7 1.8 1.9 1.4 1.5 2.0
Intensity Change (Log2) – – – -0.08 0.01 -0.29 -0.35 -0.25 -0.64 -0.73 -0.81 -0.57 -0.23 -0.26 -0.29 0.41 0.46 0.3 -0.23 -0.32 -0.22


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches -1.5 -4.0 0 0 0 0 0 0 0 0 0 0 -1.4 -3.7 -1.9
Bud -0.4 0.8 0 0 0 0 0 0 0 0 0 0 -1.6 -1.3 -0.3
Bud Neck -1.1 -0.4 0 0 0 0 0 0 0 0 0 0 -2.7 -1.8 -3.9
Bud Site -2.9 -2.8 0 0 0 0 0 0 0 0 0 0 -2.2 -3.7 -1.7
Cell Periphery 1.5 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 3.2 1.9 -2.7 3.0 5.4 5.2 2.9 3.0 4.3 4.6 10.8 10.6 1.1 1.7 1.0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 1.1 0.4 0 0.3 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole -3.5 -1.7 0 0 0 0 0 0 0 0 0 0 -2.6 -2.2 -2.4
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 0.9075 -0.995 -0.9477 -0.9657 -2.6271 -0.1279 1.6086 -0.0125 -0.1904 -0.2231 -0.5246 0.5089 0.3991 -0.7265 -0.9677 -0.6991 -1.1267 0.0363
Actin 0.1106 0.0105 0.0068 0.0441 0.0037 0.0568 0.0846 0.0139 0.047 0.0545 0.0343 0.0288 0.0889 0.0101 0.0074 0.0003 0.008 0.0357
Bud 0.0018 0.0057 0.002 0.0083 0.0003 0.0004 0.0035 0.0032 0.0028 0.0032 0.0027 0.0016 0.0014 0.0051 0.0019 0.0005 0.001 0.0007
Bud Neck 0.1072 0.0087 0.038 0.1544 0.0882 0.7763 0.1239 0.0093 0.2073 0.4511 0.1985 0.6796 0.1208 0.0117 0.1082 0.2131 0.3217 0.6934
Bud Periphery 0.0028 0.0104 0.0059 0.0146 0.001 0.0012 0.0081 0.0063 0.0058 0.0227 0.017 0.0056 0.0022 0.0165 0.0029 0.0011 0.0027 0.0017
Bud Site 0.1202 0.1674 0.0752 0.0936 0.0018 0.0022 0.1266 0.264 0.1572 0.0191 0.0031 0.0066 0.1183 0.2539 0.0811 0.0092 0.0069 0.0028
Cell Periphery 0.022 0.0172 0.0035 0.0069 0.0016 0.0013 0.0236 0.0098 0.008 0.0019 0.0016 0.0046 0.0146 0.0174 0.0036 0.0006 0.0012 0.0012
Cytoplasm 0.0887 0.1805 0.0545 0.0115 0.046 0.0366 0.1912 0.1909 0.1807 0.0706 0.1156 0.1247 0.082 0.1637 0.0966 0.11 0.0464 0.0907
Cytoplasmic Foci 0.0489 0.0091 0.035 0.0596 0.1903 0.016 0.0377 0.0109 0.0186 0.0242 0.0616 0.0051 0.0565 0.0072 0.0313 0.0117 0.0146 0.0154
Eisosomes 0.0025 0.0013 0.0022 0.0011 0.0013 0.0005 0.0013 0.001 0.0006 0.0003 0.0011 0.0004 0.0018 0.0011 0.0012 0.0001 0.0005 0.0004
Endoplasmic Reticulum 0.0153 0.0033 0.0009 0.001 0.0007 0.0121 0.0099 0.0067 0.0022 0.0013 0.0036 0.0016 0.0047 0.003 0.0044 0.0012 0.0011 0.0009
Endosome 0.0683 0.0016 0.0033 0.0018 0.0046 0.009 0.0269 0.0047 0.0061 0.0069 0.0496 0.0015 0.0277 0.0009 0.0052 0.0553 0.0078 0.0121
Golgi 0.0108 0.0005 0.0018 0.005 0.0022 0.0078 0.0122 0.0011 0.006 0.0114 0.0085 0.001 0.0045 0.0006 0.0028 0.0092 0.0076 0.0131
Lipid Particles 0.05 0.0016 0.002 0.0034 0.0022 0.0099 0.0483 0.0038 0.0054 0.0163 0.008 0.0004 0.008 0.0012 0.003 0.0026 0.0031 0.0162
Mitochondria 0.0078 0.0004 0.0006 0.0107 0.0003 0.0025 0.0118 0.0011 0.0087 0.0024 0.0353 0.0009 0.0023 0.0005 0.0016 0.0185 0.0623 0.0165
None 0.2299 0.5749 0.7613 0.5708 0.6467 0.0515 0.2185 0.4611 0.3288 0.296 0.4156 0.1328 0.3925 0.4992 0.6394 0.531 0.5071 0.0865
Nuclear Periphery 0.0487 0.0005 0.0002 0.0005 0.0001 0.0087 0.0146 0.001 0.0006 0.0004 0.0047 0.0003 0.0189 0.0004 0.0008 0.0006 0.0007 0.0012
Nucleolus 0.0014 0.0007 0.0007 0.0011 0.0003 0.0003 0.0027 0.0015 0.0008 0.0004 0.0013 0.0007 0.0009 0.001 0.0006 0.0006 0.0003 0.0004
Nucleus 0.0264 0.0012 0.0005 0.001 0.0004 0.0008 0.0163 0.0019 0.0018 0.0008 0.0029 0.001 0.0049 0.0019 0.001 0.0009 0.0006 0.0006
Peroxisomes 0.0192 0.0004 0.0031 0.0045 0.0022 0.0017 0.0227 0.002 0.0075 0.0142 0.0047 0.0007 0.0056 0.0006 0.0039 0.0004 0.0008 0.0069
Punctate Nuclear 0.0091 0.0017 0.002 0.0046 0.006 0.0024 0.0055 0.0024 0.0016 0.0011 0.0223 0.0009 0.0356 0.002 0.0011 0.0003 0.0011 0.0023
Vacuole 0.0051 0.0023 0.0005 0.0009 0.0002 0.0013 0.0079 0.0032 0.0022 0.0009 0.0052 0.0014 0.0048 0.0018 0.0018 0.0235 0.0018 0.0008
Vacuole Periphery 0.0034 0.0001 0.0001 0.0003 0 0.0006 0.0023 0.0002 0.0002 0.0002 0.0025 0.0001 0.0032 0.0001 0.0002 0.0091 0.0026 0.0005

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 13.7024 13.3236 7.3353 9.9347 13.7139 10.5714 17.0566 15.8383 12.6308 16.8191
Translational Efficiency 0.8108 1.0333 1.1087 0.7271 0.7412 0.7699 0.7585 0.641 0.6846 0.6947

Hit Data

Dataset Hit
Protein Concentration ✔
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
257 80 169 1429 61 85 1242 1638 318 165 1411 3067

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 706.46 774.06 925.00 929.59 592.27 776.67 815.90 791.38 684.56 775.40 828.97 855.78
Standard Deviation 90.56 90.27 106.35 134.74 74.83 116.48 123.84 111.25 98.61 104.60 126.92 140.79
Intensity Change Log 2 — 0.131838 0.388845 0.395987 — 0.391047 0.462137 0.418116 — 0.255846 0.422732 0.406120

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.001177 0.007625 0.003584 0.023915 0.000559 0.001012 0.002643 0.002348 0.001059 0.004218 0.002756 0.012397
Bud Neck 0.243272* 0.137070 0.219287* 0.208305* 0.203417* 0.102907 0.175127 0.280385* 0.235627* 0.119471 0.180416* 0.246801*
Bud Site 0.169672* 0.142622* 0.244434* 0.478874* 0.052034 0.101558* 0.210141* 0.289797* 0.147107* 0.121468* 0.214248* 0.377893*
Cell Periphery 0.004933 0.005236 0.003590 0.009348 0.000999 0.000552 0.000988 0.002288 0.004178 0.002823 0.001300 0.005577
Cytoplasm 0.256905* 0.234155* 0.147833* 0.103725* 0.274347* 0.173416* 0.303857* 0.178417* 0.260251* 0.202865* 0.285170* 0.143616*
Cytoplasmic Foci 0.109968 0.192566* 0.055001 0.016687 0.243002* 0.208646* 0.036380 0.017116 0.135487 0.200850* 0.038610 0.016916
Eisosomes 0.002410 0.001217 0.000567 0.001826 0.000554 0.000260 0.000263 0.000362 0.002054 0.000724 0.000300 0.001044
Endoplasmic Reticulum 0.000973 0.002278 0.008437 0.004798 0.000636 0.015012 0.001061 0.000954 0.000908 0.008838 0.001944 0.002745
Endosome 0.013619 0.057396 0.023276 0.007027 0.028181 0.078363 0.007642 0.005380 0.016413 0.068197 0.009514 0.006147
Golgi 0.002012 0.013467 0.006196 0.004239 0.007544 0.011735 0.002991 0.001411 0.003073 0.012575 0.003375 0.002729
Lipid Particles 0.005410 0.017511 0.007158 0.001248 0.009515 0.017698 0.001848 0.000686 0.006198 0.017607 0.002484 0.000948
Mitochondria 0.001205 0.014642 0.000379 0.003361 0.041574 0.018094 0.000894 0.001580 0.008948 0.016420 0.000833 0.002410
Mitotic Spindle 0.000439 0.001858 0.001027 0.007283 0.000504 0.008046 0.006274 0.004287 0.000452 0.005046 0.005646 0.005683
None 0.003416 0.000538 0.000652 0.000186 0.002095 0.000449 0.006690 0.000512 0.003162 0.000492 0.005967 0.000360
Nuclear Periphery 0.000154 0.000242 0.000805 0.000151 0.000178 0.001524 0.000260 0.000207 0.000159 0.000903 0.000325 0.000181
Nuclear Periphery Foci 0.000307 0.001349 0.004427 0.001207 0.000526 0.001419 0.000643 0.000205 0.000349 0.001385 0.001096 0.000672
Nucleolus 0.000626 0.001607 0.001079 0.000159 0.002171 0.003983 0.000495 0.000208 0.000923 0.002831 0.000565 0.000185
Nucleus 0.058465 0.036351 0.110567 0.024615 0.068823 0.054621 0.086045 0.074083 0.060452 0.045763 0.088982 0.051035
Peroxisomes 0.006395 0.077812 0.006557 0.008302 0.028172 0.036368 0.003551 0.002326 0.010572 0.056462 0.003911 0.005110
Vacuole 0.117773 0.051846 0.154306 0.093654 0.034082 0.158826 0.151255 0.137142 0.101719 0.106957 0.151621 0.116880
Vacuole Periphery 0.000869 0.002611 0.000839 0.001092 0.001089 0.005510 0.000953 0.000304 0.000911 0.004104 0.000939 0.000671

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -2.94 -3.09 -11.36 -5.39 -9.71 -1.19 -2.62 -4.11 -2.93 -0.11 -2.82 -2.24 -11.40 -5.43 -9.36
Bud Neck 3.62 1.82 3.02 -2.03 0.43 2.19 1.89 -0.80 -5.60 -11.56 4.98 5.58 1.29 -5.54 -9.94
Bud Site 0.95 -1.63 -12.67 -11.53 -9.15 -1.56 -5.86 -10.99 -5.96 -8.67 1.26 -0.91 -11.12 -11.97 -18.40
Cell Periphery -0.06 1.16 -2.31 -1.46 -4.89 0.93 0.54 -1.84 -6.88 -6.97 1.01 3.10 -0.68 -2.01 -10.12
Cytoplasm 0.84 4.61 8.35 4.01 1.57 2.18 -0.30 2.22 -0.55 9.05 2.44 -0.68 6.80 2.31 12.55
Cytoplasmic Foci -3.82 4.58 9.56 8.80 5.58 0.82 6.88 7.33 8.28 6.14 -3.68 9.86 11.60 12.00 7.98
Eisosomes 2.50 4.27 1.59 -1.57 -5.40 1.56 1.80 1.18 -1.34 -3.00 3.63 5.11 3.04 -1.60 -7.36
Endoplasmic Reticulum -1.51 -4.97 -6.91 -2.75 2.36 -1.25 -2.32 -1.91 1.20 0.68 -1.34 -4.56 -6.51 0.96 -2.22
Endosome -3.44 -2.13 4.12 4.08 4.04 -2.82 2.38 2.50 4.98 1.31 -5.14 3.72 4.87 6.44 2.70
Golgi -4.14 -0.87 -1.20 3.51 0.49 -0.71 2.30 2.75 2.22 0.98 -3.27 0.81 1.17 3.69 0.15
Lipid Particles -1.69 -2.38 5.34 2.20 6.59 -0.98 3.68 4.09 2.20 3.25 -2.15 5.08 7.15 3.12 4.62
Mitochondria -2.47 2.75 -1.68 2.11 -2.60 1.14 2.18 2.14 1.50 -1.67 -0.96 2.25 1.84 2.28 -2.81
Mitotic Spindle -0.32 0.44 -3.77 -4.14 -5.13 -1.29 -4.48 -3.42 0.70 1.81 -1.20 -3.33 -3.81 -0.22 0.46
None 1.10 1.06 1.21 1.72 2.57 2.15 -2.17 1.99 -0.65 4.02 1.27 -0.61 1.31 0.61 4.18
Nuclear Periphery -0.92 -5.44 -2.56 -0.07 4.70 -1.22 -5.86 -4.25 1.02 2.77 -1.31 -10.22 -5.82 1.00 6.00
Nuclear Periphery Foci -0.91 -6.12 -2.91 -0.18 4.60 -1.90 -0.61 0.70 3.47 7.65 -1.78 -3.61 -1.57 1.11 2.37
Nucleolus -0.64 -0.40 1.63 1.28 3.51 -0.86 1.44 1.59 2.15 3.42 -1.55 1.73 2.35 2.50 3.05
Nucleus 2.00 -4.26 3.59 0.34 6.20 0.70 -1.71 -1.76 -3.20 -0.13 1.43 -5.55 -1.21 -2.68 6.64
Peroxisomes -4.00 0.32 -0.32 3.98 -1.09 -0.54 3.37 3.46 3.05 1.31 -4.25 3.18 2.51 4.96 -2.47
Vacuole 3.41 -3.54 -2.35 -5.86 2.38 -4.96 -15.05 -10.57 -0.86 7.92 -0.86 -13.56 -6.81 -3.51 10.36
Vacuole Periphery -1.82 0.27 -0.03 1.93 -0.38 -2.27 0.99 2.33 2.78 1.66 -2.81 0.65 0.98 3.32 0.41
Protein Complexes26S Proteasome complex37S mitochondrial small ribosomal subunit54S mitochondrial large ribosomal subunit60S cytosolic large ribosomal subunitAar2-U5 snRNPAnaphase-Promoting Complex variant 1Anaphase-Promoting Complex variant 2Anaphase-Promoting Complex variant 3Anaphase-Promoting Complex variant 4Arp2/3 complexATG1 kinase complexBLOC-1 complexCcr4-Not complexCentral kinetochore CTF19 complexCleavage and polyadenylation specificity factorcomplexCMG helicase complexCMG-Pol epsilon complexCOMPASS complexCOP9 signalosome COPI vesicle coat complexCoQ6 biosynthesis complexCore mediator complexCORVET complexCTF18-RFC complexCURI complex variant 1CURI complex variant 2CURI complex variant 3Cytoplasmic exosome complex, DIS3 variantDASH complexDNA-directed RNA Polymerase I complexDNA-directed RNA polymerase II complexDNA-directed RNA polymerase III complexDNA replication factor C complexEGO complexELG1-RFC complex Elongator holoenzyme complexEndoplasmic Reticulum Membrane ComplexEukaryotic translation initiation factor 2BcomplexEukaryotic translation initiation factor 3core complexExocystExomer complexFAR complexGeneral transcription factor complex TFIIDGeneral transcription factor complex TFIIHGET4-GET5 transmembrane domain recognition complexGID ubiquitin ligase complexGin4 complexGolgi transport complexGSE complexHMC complexHOPS complexINO80 chromatin remodeling complexLSM1-7 complexLsm1-7-Pat1 complexLSM2-7 complexLSM2-8 complexMCM complexMICOS complexMitochondrial electron transport complex IIIMitochondrial inner membrane pre-sequence translocasecomplexMitochondrial outer membrane translocase holocomplexMitochondrial proton-transporting ATP synthase complexMitochondrial pyruvate dehydrogenase complexNineTeen complexNOP8 60s ribosome pre-assembly complexNuA3 histone acetyltransferase complexNuA4 histone acetyltransferase complexNuclear condensin complexNuclear/nucleolar exosome complex, DIS3-RRP6 variantNuclear pore complexNucleolar ribonuclease MRP complexNucleolar ribonuclease P complexOligosaccharyl transferase complex variant 1Oligosaccharyl transferase complex variant 2PAF1 complexPAS complexPhosphatidylinositol 3-kinase complex IPolarisomePrefoldin complexPRP19-associated complexRad17 RFC-like complexRetromer complexRPD3L histone deacetylase complexRPD3S histone deacetylase complexRQC complexRSC complex variant RSC1RSC complex variant RSC2SAGA complexSCF-Met30 ubiquitin ligase complexSCF-Ufo1 ubiquitin ligase complexSEA complexSeptin complexSET3C histone deacetylase complexSignal recognition particleSLIK (SAGA-like) complexSmall ribosomal subunit processome, variant1Small ribosomal subunit processome, variant2Small ribosomal subunit processome, variant3SMC5-SMC6 SUMO ligase complexSm complexSpliceosomal commitment complexSPOTS complexSWI/SNF chromatin remodelling complexSwr1 chromatin remodelling complexTHO complexTranscription factor TFIIIC complexTranslocon complexTRAPPIII protein complexTRAPPII protein complexTRAPPI protein complexTREX complexU1 snRNPU2 snRNPU4 snRNPU4/U6 snRNPU4/U6.U5 tri-snRNP complexU5 snRNPU6 snRNP complexUTP-A complexUTP-B complexUTP-C complex variant 1UTP-C complex variant 2UTP-C complex variant 3Vacuolar proton translocating ATPase complex,Golgi variantVacuolar proton translocating ATPase complex,vacuole variantCellular Components••••••••••••••••actinbud neckcell peripherycytoplasmendosomeERGolgimitochondrionmixednuclear peripherynucleolusnucleusperoxisomespindle polevacuolar membranevacuole
Standard name
Human Ortholog
Description Dual-specificity kinase; autophosphorylation required for function; required for spindle pole body (SPB) duplication and spindle checkpoint function; contributes to bi-orientation by promoting formation of force-generating kinetochore-microtubule attachments in meiosis I; substrates include SPB proteins Spc42p, Spc110p, and Spc98p, mitotic exit network protein Mob1p, kinetochore protein Cnn1p, and checkpoint protein Mad1p; substrate of APCC(Cdh1); similar to human Mps1p
Localization
Cell Percentages cytoplasm (13%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-10

Mps1

Mps1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Mps1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available