Standard name
Human Ortholog
Description tRNA-specific 2-thiouridylase; responsible for 2-thiolation of the wobble base of mitochondrial tRNAs; human homolog TRMU is implicated in myoclonus epilepsy associated with ragged red fibers (MERRF), and can complement yeast null mutant

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0
Bud 0.07 0 0 0.05 0 0 0 0 0 0 0.06 0 0.05 0 0 0.05 0 0.1 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.06 0.07 0 0 0 0 0 0
Cytoplasm 0 0.2 0.12 0.05 0.05 0 0 0 0 0 0 0 0.24 0.3 0.37 0.1 0.19 0.13 0 0 0 0.06 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0 0
Endosome 0 0.09 0.08 0.06 0.11 0 0 0 0 0 0 0 0.17 0.25 0.23 0.26 0.13 0.29 0.14 0.12 0.11 0.1 0.08 0.09
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.06 0 0 0 0 0 0
Mitochondria 0.86 0.62 0.65 0.89 0.8 0.87 0.9 0.88 0.93 0.95 0.96 0.96 0.32 0.08 0.06 0.14 0.16 0.12 0.65 0.59 0.68 0.57 0.58 0.55
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0.07 0.23 0.14 0.15 0.09 0 0 0 0 0 0 0.09 0.07 0.1 0.23 0.22 0.2 0.06 0 0.09 0 0 0
SpindlePole 0.11 0.13 0.14 0.1 0.05 0.08 0.08 0.08 0 0.05 0 0 0.18 0.28 0.27 0.31 0.3 0.27 0.05 0.09 0 0.07 0.09 0.07
Vac/Vac Membrane 0.06 0.11 0.06 0 0.08 0.08 0 0 0 0 0 0 0.1 0.16 0.09 0.15 0.15 0.18 0 0.07 0 0.06 0.09 0.09
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 2 2 0 0 0 0 0 0 0 0 0 1 22 4 5 1 0 1 3 0 2 3 10 14
Bud 14 4 9 11 12 13 29 24 21 27 29 17 22 12 8 8 4 14 0 2 0 2 7 10
Bud Neck 0 0 0 0 0 0 0 1 1 0 0 0 6 6 6 0 1 0 1 2 1 4 10 17
Bud Site 1 1 2 3 0 6 23 17 6 13 10 10 1 0 2 2 3 3
Cell Periphery 0 0 6 3 3 4 3 3 6 2 1 6 16 5 3 8 9 10 0 0 0 0 1 1
Cytoplasm 7 26 28 10 23 15 19 13 7 12 11 6 112 138 196 16 27 19 1 5 1 12 21 18
Endoplasmic Reticulum 6 1 2 1 2 1 2 1 3 3 1 7 1 3 10 3 9 4 1 0 0 0 3 4
Endosome 5 12 18 12 46 11 5 29 5 0 0 2 81 116 121 40 19 42 32 18 26 21 48 59
Golgi 0 0 1 2 0 0 0 0 0 0 0 0 3 3 6 7 7 9 2 0 6 2 5 4
Mitochondria 178 81 148 194 340 350 653 711 496 715 454 608 151 35 34 22 23 18 149 91 167 123 343 369
Nucleus 1 0 1 1 3 11 28 27 10 13 13 4 1 1 1 2 4 3 0 1 0 2 4 8
Nuclear Periphery 2 0 0 0 0 8 17 21 12 19 8 23 0 0 0 1 2 1 1 0 1 0 1 4
Nucleolus 1 1 2 0 4 7 26 15 14 23 8 11 0 1 1 3 2 3 1 1 0 0 3 5
Peroxisomes 7 9 52 30 64 38 23 34 6 19 2 5 41 33 50 36 32 30 13 2 22 3 15 29
SpindlePole 23 17 32 22 23 33 58 63 21 40 12 21 86 129 144 48 43 39 10 13 8 15 50 44
Vac/Vac Membrane 12 14 14 6 34 32 25 36 23 18 18 23 46 72 47 24 21 26 4 10 2 13 52 63
Unique Cell Count 206 130 226 218 424 404 726 808 531 749 473 631 472 464 526 156 144 147 229 155 246 215 590 673
Labelled Cell Count 259 168 315 295 554 529 911 995 631 904 567 744 589 558 634 221 206 222 229 155 246 215 590 673


Mitochondria

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 3.9 4.3 4.7 4.4 4.6 4.1 4.0 4.0 3.9 3.8 3.5 3.7 5.7 5.6 5.5 6.1 6.7 6.4 5.5 5.6 6.1
Std Deviation (1e-4) 0.6 0.8 1.7 1.8 1.6 1.5 1.3 0.9 0.9 1.0 0.8 0.9 0.8 0.7 1.0 1.4 1.7 1.6 1.0 1.1 1.4
Intensity Change (Log2) -0.09 -0.04 -0.19 -0.24 -0.23 -0.26 -0.3 -0.42 -0.34 0.27 0.25 0.23 0.38 0.5 0.44 0.22 0.25 0.37

WT3RAP60RAP140RAP220RAP300RAP380RAP460RAP540RAP620RAP70002468WT3HU80HU120HU16002468WT3rpd3Δ_1rpd3Δ_2rpd3Δ_302468WT1AF100AF140AF18002468
rapamycinhydroxy-urearpd3 knockoutalpha-factorMultiple Condition Time-Course Intensity PlotsGFP Intensity (1e-4)GFP Intensity (1e-4)GFP Intensity (1e-4)GFP Intensity (1e-4)

Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin
Bud 0 0 0
Bud Neck 0 0 0
Bud Site 0 0 0
Cell Periphery 0 0 0
Cyto
Endoplasmic Reticulum 0 0 0
Endosome 0 0 0
Golgi 0 0 0
Mitochondria 0 0 0
Nuclear Periphery 0 0 0
Nuc
Nucleolus 0 0 0
Peroxisomes 0 0 0
SpindlePole 0 0 0
Vac
Cortical Patches 0 0 0
Cytoplasm 0 0 0
Nucleus 0 0 0
Vacuole 2.9 2.7 3.5

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 1.8516 2.3013 2.3318 2.1557 2.0501 2.3044 0.0766 0.3533 0.142 0.0466 0.2373 0.1172 0.2884 0.4198 0.3066 0.3076 0.4112 0.1276
Actin 0.0481 0.045 0.0128 0.0189 0.0065 0.0343 0.0919 0.0254 0.0328 0.0334 0.0053 0.0219 0.0289 0.0174 0.0326 0.0223 0.0793 0.0105
Bud 0.0003 0.0018 0.0018 0.0023 0.0072 0.0007 0.0018 0.0019 0.0033 0.0071 0.0013 0.001 0.0009 0.0019 0.0061 0.0051 0.0219 0.001
Bud Neck 0.0004 0.0013 0.0063 0.0152 0.0004 0.0023 0.0059 0.0033 0.0008 0.0011 0.0002 0.0023 0.0043 0.0024 0.002 0.001 0.0029 0.0008
Bud Periphery 0.0007 0.003 0.0052 0.0135 0.026 0.002 0.003 0.0027 0.004 0.0273 0.0077 0.0024 0.0021 0.0026 0.0084 0.0053 0.0236 0.0017
Bud Site 0.0013 0.0401 0.022 0.0097 0.0059 0.0016 0.0283 0.0239 0.0096 0.0054 0.0003 0.0049 0.0056 0.0268 0.0172 0.0129 0.0091 0.0008
Cell Periphery 0.0003 0.0014 0.0029 0.0494 0.0007 0.0015 0.0013 0.001 0.0012 0.0061 0.0003 0.001 0.0008 0.0016 0.0019 0.0005 0.001 0.0006
Cytoplasm 0.0009 0.0024 0.0052 0.0052 0.0021 0.003 0.0021 0.0062 0.0115 0.0037 0.0009 0.0132 0.0056 0.0044 0.0038 0.002 0.0097 0.0015
Cytoplasmic Foci 0.0152 0.0264 0.041 0.0212 0.0174 0.0281 0.0298 0.0431 0.0422 0.0169 0.0082 0.0096 0.0325 0.0491 0.034 0.0238 0.0479 0.0289
Eisosomes 0.0007 0.0006 0.0021 0.0009 0.0008 0.0009 0.001 0.0004 0.0007 0.0004 0.0002 0.0009 0.0013 0.0004 0.0005 0.0003 0.0005 0.0004
Endoplasmic Reticulum 0.0018 0.0034 0.0018 0.0009 0.0005 0.0025 0.0028 0.0007 0.0019 0.0005 0.0018 0.0065 0.0044 0.0013 0.0014 0.0014 0.0017 0.0013
Endosome 0.071 0.0446 0.0398 0.0972 0.0884 0.0508 0.0593 0.0732 0.0619 0.0277 0.0874 0.0416 0.0975 0.0771 0.0872 0.0503 0.1003 0.05
Golgi 0.0399 0.0655 0.0301 0.0434 0.0349 0.0489 0.0563 0.0643 0.0775 0.0531 0.1115 0.0415 0.0464 0.0552 0.0352 0.0338 0.107 0.0373
Lipid Particles 0.0543 0.0269 0.0208 0.0147 0.0052 0.0211 0.0449 0.0317 0.0181 0.015 0.0375 0.0169 0.0946 0.0544 0.0178 0.0213 0.0183 0.0376
Mitochondria 0.6962 0.6372 0.7354 0.6417 0.7179 0.7138 0.6 0.6507 0.6631 0.7754 0.7263 0.8029 0.5586 0.5919 0.6874 0.7924 0.4826 0.769
None 0.0009 0.0006 0.0093 0.0019 0.0012 0.0277 0.0014 0.0006 0.0014 0.0007 0.0004 0.0013 0.0037 0.0093 0.0099 0.001 0.0067 0.0013
Nuclear Periphery 0.0109 0.0007 0.0005 0.0004 0.0003 0.0019 0.006 0.0004 0.0019 0.0003 0.0011 0.0049 0.0104 0.0015 0.0019 0.0005 0.0066 0.0023
Nucleolus 0.0004 0.0005 0.0008 0.0011 0.0004 0.0005 0.001 0.0025 0.0004 0.0003 0.0001 0.0005 0.0046 0.0036 0.0023 0.0009 0.0015 0.0008
Nucleus 0.0007 0.0005 0.0006 0.0011 0.0005 0.0004 0.0154 0.0011 0.0008 0.0005 0.0002 0.0009 0.0276 0.0013 0.0025 0.0006 0.0033 0.0006
Peroxisomes 0.0348 0.0891 0.0424 0.0306 0.065 0.0459 0.0345 0.0356 0.0575 0.018 0.0023 0.0148 0.0365 0.0676 0.0256 0.0144 0.0456 0.0398
Punctate Nuclear 0.0003 0.0002 0.0004 0.0003 0.0001 0.0013 0.0015 0.0003 0.0046 0.0002 0.0003 0.0017 0.0034 0.0005 0.0025 0.0002 0.006 0.0016
Vacuole 0.0086 0.0056 0.0133 0.0218 0.0109 0.006 0.0061 0.0212 0.0024 0.0034 0.0022 0.0036 0.0192 0.0166 0.0126 0.0042 0.0135 0.0045
Vacuole Periphery 0.0122 0.0031 0.0054 0.0083 0.0078 0.0048 0.0058 0.0099 0.0024 0.0033 0.0046 0.0057 0.0111 0.013 0.0072 0.0058 0.011 0.0077

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 14.5185 11.7988 8.1715 9.3281 12.5492 8.7942 11.9297 10.7357 14.4301 12.4903
Translational Efficiency 0.373 0.4175 0.5416 0.3759 0.4385 0.5068 0.5455 0.5361 0.2737 0.5042

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
965 581 2380 658 1442 288 218 478 2407 869 2598 1136

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 638.14 735.59 818.46 885.69 650.44 715.21 963.89 889.17 645.51 728.84 830.66 887.15
Standard Deviation 107.32 117.45 96.81 104.13 85.52 88.56 116.49 125.04 95.05 109.15 106.53 113.41
Intensity Change Log 2 0.205029 0.359039 0.472929 0.136951 0.567453 0.451043 0.171067 0.467999 0.461923

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.001448 0.007239 0.003895 0.021849 0.001708 0.001920 0.015849 0.044838 0.001604 0.005476 0.004898 0.031523
Bud Neck 0.027148 0.016716 0.006952 0.010282 0.018293 0.012722 0.015725 0.014545 0.021843 0.015392 0.007688 0.012076
Bud Site 0.017450 0.035295 0.042473 0.080941 0.028842 0.017651 0.077398 0.153666 0.024275 0.029447 0.045403 0.111542
Cell Periphery 0.000880 0.000635 0.000368 0.000413 0.000357 0.000347 0.000433 0.002049 0.000567 0.000540 0.000373 0.001101
Cytoplasm 0.021017 0.009778 0.007820 0.015823 0.024740 0.008424 0.009481 0.023466 0.023247 0.009329 0.007960 0.019039
Cytoplasmic Foci 0.093026 0.086597 0.033880 0.097787 0.113908 0.142737 0.120642 0.060464 0.105537 0.105202 0.041160 0.082082
Eisosomes 0.000198 0.000177 0.000084 0.000151 0.000139 0.000180 0.000167 0.000249 0.000163 0.000178 0.000091 0.000192
Endoplasmic Reticulum 0.001897 0.000862 0.000948 0.002075 0.001560 0.000680 0.004277 0.002951 0.001695 0.000802 0.001227 0.002444
Endosome 0.087202 0.130170 0.084396 0.114726 0.083305 0.109983 0.136300 0.062438 0.084867 0.123480 0.088751 0.092724
Golgi 0.116334 0.133453 0.064970 0.092569 0.121488 0.164170 0.097517 0.076023 0.119422 0.143633 0.067701 0.085607
Lipid Particles 0.012273 0.005279 0.000723 0.001873 0.009700 0.006988 0.008008 0.000749 0.010731 0.005845 0.001335 0.001400
Mitochondria 0.488962 0.404065 0.610808 0.252395 0.489515 0.395949 0.171569 0.279782 0.489293 0.401375 0.573951 0.263919
Mitotic Spindle 0.007048 0.024898 0.044566 0.113993 0.012885 0.012071 0.117104 0.063517 0.010545 0.020647 0.050652 0.092754
None 0.002008 0.001040 0.000255 0.000173 0.000920 0.000316 0.000168 0.000211 0.001356 0.000800 0.000247 0.000189
Nuclear Periphery 0.000493 0.000537 0.000136 0.001621 0.000256 0.000084 0.000249 0.000074 0.000351 0.000387 0.000146 0.000970
Nuclear Periphery Foci 0.000398 0.000763 0.000205 0.001245 0.000370 0.000204 0.001202 0.000224 0.000381 0.000578 0.000289 0.000815
Nucleolus 0.002309 0.001557 0.000447 0.000353 0.001943 0.000559 0.000762 0.000109 0.002089 0.001226 0.000473 0.000250
Nucleus 0.005762 0.002964 0.001267 0.001956 0.004162 0.001394 0.005626 0.002448 0.004803 0.002444 0.001633 0.002163
Peroxisomes 0.085385 0.105008 0.043237 0.117151 0.049596 0.106179 0.159899 0.175973 0.063944 0.105396 0.053026 0.141902
Vacuole 0.013409 0.021672 0.036256 0.055633 0.020051 0.009998 0.048263 0.027963 0.017388 0.017803 0.037263 0.043990
Vacuole Periphery 0.015352 0.011295 0.016315 0.016992 0.016263 0.007445 0.009363 0.008258 0.015898 0.010019 0.015732 0.013317

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -3.51 -4.48 -6.43 -4.10 -5.60 -0.31 -4.09 -8.47 -8.39 -4.78 -3.40 -5.58 -10.52 -8.56 -9.23
Bud Neck 2.65 6.15 4.88 2.46 -2.87 1.40 0.48 1.48 -0.48 0.55 2.55 8.03 5.00 1.44 -3.87
Bud Site -4.34 -9.41 -10.95 -6.90 -6.53 2.47 -4.96 -12.79 -13.26 -5.56 -1.59 -8.22 -16.03 -13.85 -11.74
Cell Periphery 1.06 2.29 2.09 2.83 -0.82 0.16 -1.04 -3.73 -3.73 -3.55 0.27 2.08 -2.47 -2.80 -3.73
Cytoplasm 3.89 5.39 0.94 -3.56 -5.71 6.41 5.58 0.27 -4.53 -3.93 7.18 9.23 1.19 -5.61 -7.34
Cytoplasmic Foci 1.06 14.90 -0.56 -1.48 -13.19 -2.91 -0.71 9.86 8.18 6.16 0.02 20.92 5.86 4.40 -11.38
Eisosomes 0.99 6.09 2.31 1.81 -6.71 -1.38 -1.80 -6.16 -2.13 -3.78 -1.01 7.94 -2.36 -0.95 -10.64
Endoplasmic Reticulum 1.70 1.60 -0.60 -3.19 -3.21 2.03 -2.74 -2.08 -3.53 1.16 2.49 1.23 -2.01 -4.68 -3.51
Endosome -5.19 0.50 -3.36 1.86 -4.21 -2.70 -4.28 3.48 4.59 5.83 -6.21 -0.97 -1.32 4.61 -0.56
Golgi -1.89 8.76 3.53 4.96 -4.39 -3.54 2.42 6.39 7.10 1.77 -3.46 12.43 6.88 8.40 -3.69
Lipid Particles 3.37 6.80 5.99 2.63 -3.86 1.47 0.50 7.26 4.69 2.91 3.60 9.04 9.13 4.68 -0.32
Mitochondria 4.67 -8.80 14.27 8.36 25.47 4.10 16.02 11.68 4.54 -4.74 6.40 -8.13 18.89 9.30 26.09
Mitotic Spindle -5.28 -14.68 -12.69 -10.00 -8.03 0.35 -8.20 -6.84 -6.84 3.73 -4.17 -15.54 -14.06 -11.68 -6.73
None 1.35 4.02 4.20 1.71 4.76 2.40 3.33 3.17 0.81 -1.79 1.31 5.03 5.28 1.78 3.92
Nuclear Periphery -0.09 2.31 -1.02 -0.91 -1.35 2.46 -0.14 2.67 0.11 3.06 -0.12 2.72 -0.97 -0.83 -1.29
Nuclear Periphery Foci -0.81 1.85 -2.11 -0.91 -2.58 1.93 -3.38 2.22 -0.22 3.92 -0.66 0.98 -1.88 -0.72 -2.15
Nucleolus 0.83 2.88 3.31 2.05 0.68 2.39 2.01 3.53 1.69 2.52 1.51 3.54 4.65 2.44 1.46
Nucleus 1.95 3.58 1.68 -0.12 -1.86 2.91 -0.53 0.93 -1.14 0.94 2.72 3.91 1.75 -0.60 -1.62
Peroxisomes -2.10 6.84 -3.11 -0.92 -8.83 -5.23 -6.59 -10.02 -4.29 -0.77 -6.13 2.71 -10.24 -3.79 -11.85
Vacuole -2.96 -10.59 -9.78 -7.02 -5.02 4.74 -4.94 -2.33 -4.78 2.98 -0.56 -10.39 -9.16 -7.82 -2.94
Vacuole Periphery 2.09 -0.54 -0.53 -2.58 -0.14 4.70 3.83 4.48 -0.38 0.48 4.60 0.12 1.87 -2.08 1.81
Protein Complexes26S Proteasome complex37S mitochondrial small ribosomal subunit54S mitochondrial large ribosomal subunit60S cytosolic large ribosomal subunitAar2-U5 snRNPAnaphase-Promoting Complex variant 1Anaphase-Promoting Complex variant 2Anaphase-Promoting Complex variant 3Anaphase-Promoting Complex variant 4Arp2/3 complexATG1 kinase complexBLOC-1 complexCcr4-Not complexCentral kinetochore CTF19 complexCleavage and polyadenylation specificity factorcomplexCMG helicase complexCMG-Pol epsilon complexCOMPASS complexCOP9 signalosome COPI vesicle coat complexCoQ6 biosynthesis complexCore mediator complexCORVET complexCTF18-RFC complexCURI complex variant 1CURI complex variant 2CURI complex variant 3Cytoplasmic exosome complex, DIS3 variantDASH complexDNA-directed RNA Polymerase I complexDNA-directed RNA polymerase II complexDNA-directed RNA polymerase III complexDNA replication factor C complexEGO complexELG1-RFC complex Elongator holoenzyme complexEndoplasmic Reticulum Membrane ComplexEukaryotic translation initiation factor 2BcomplexEukaryotic translation initiation factor 3core complexExocystExomer complexFAR complexGeneral transcription factor complex TFIIDGeneral transcription factor complex TFIIHGET4-GET5 transmembrane domain recognition complexGID ubiquitin ligase complexGin4 complexGolgi transport complexGSE complexHMC complexHOPS complexINO80 chromatin remodeling complexLSM1-7 complexLsm1-7-Pat1 complexLSM2-7 complexLSM2-8 complexMCM complexMICOS complexMitochondrial electron transport complex IIIMitochondrial inner membrane pre-sequence translocasecomplexMitochondrial outer membrane translocase holocomplexMitochondrial proton-transporting ATP synthase complexMitochondrial pyruvate dehydrogenase complexNineTeen complexNOP8 60s ribosome pre-assembly complexNuA3 histone acetyltransferase complexNuA4 histone acetyltransferase complexNuclear condensin complexNuclear/nucleolar exosome complex, DIS3-RRP6 variantNuclear pore complexNucleolar ribonuclease MRP complexNucleolar ribonuclease P complexOligosaccharyl transferase complex variant 1Oligosaccharyl transferase complex variant 2PAF1 complexPAS complexPhosphatidylinositol 3-kinase complex IPolarisomePrefoldin complexPRP19-associated complexRad17 RFC-like complexRetromer complexRPD3L histone deacetylase complexRPD3S histone deacetylase complexRQC complexRSC complex variant RSC1RSC complex variant RSC2SAGA complexSCF-Met30 ubiquitin ligase complexSCF-Ufo1 ubiquitin ligase complexSEA complexSeptin complexSET3C histone deacetylase complexSignal recognition particleSLIK (SAGA-like) complexSmall ribosomal subunit processome, variant1Small ribosomal subunit processome, variant2Small ribosomal subunit processome, variant3SMC5-SMC6 SUMO ligase complexSm complexSpliceosomal commitment complexSPOTS complexSWI/SNF chromatin remodelling complexSwr1 chromatin remodelling complexTHO complexTranscription factor TFIIIC complexTranslocon complexTRAPPIII protein complexTRAPPII protein complexTRAPPI protein complexTREX complexU1 snRNPU2 snRNPU4 snRNPU4/U6 snRNPU4/U6.U5 tri-snRNP complexU5 snRNPU6 snRNP complexUTP-A complexUTP-B complexUTP-C complex variant 1UTP-C complex variant 2UTP-C complex variant 3Vacuolar proton translocating ATPase complex,Golgi variantVacuolar proton translocating ATPase complex,vacuole variantCellular Componentsactinbud neckcell peripherycytoplasmendosomeERGolgimitochondrionmixednuclear peripherynucleolusnucleusperoxisomespindle polevacuolar membranevacuole
Standard name
Human Ortholog
Description tRNA-specific 2-thiouridylase; responsible for 2-thiolation of the wobble base of mitochondrial tRNAs; human homolog TRMU is implicated in myoclonus epilepsy associated with ragged red fibers (MERRF), and can complement yeast null mutant
Localization
Cell Percentages mitochondrion (86%)
Cell Cycle Regulation No
Subcompartmental Group mito-1

Slm3

Slm3


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)

Molecular Function (Gene Ontology)

Cellular Component (Gene Ontology)


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Slm3-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available