Standard name
Human Ortholog
Description Mitochondrial tRNA:pseudouridine synthase; catalyzes the formation of pseudouridine at position 32 in mitochondrial tRNAs; contains an N-terminal mitochondrial targeting sequence; PUS9 has a paralog, RIB2, that arose from the whole genome duplication
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Cytoplasm 0.05 0.11 0 0.11 0.13 0.19 0.14 0.2 0.12 0.19 0.11 0.14 0.12 0.23 0.25 – 0.26 0.3 0 0.05 0 0 0.06 0.05
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0.06 0 0 0 0 0 0 0
Endosome 0.07 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Mitochondria 0.22 0.22 0.46 0.53 0.62 0.67 0.8 0.76 0.84 0.77 0.84 0.75 0.08 0.08 0 – 0.07 0.07 0.05 0 0 0.05 0.12 0.11
Nucleus 0.56 0.47 0.5 0.58 0.43 0.32 0.28 0.35 0.31 0.25 0.37 0.35 0.75 0.54 0.49 – 0.3 0.45 0.48 0.55 0.55 0.51 0.35 0.17
Nuclear Periphery 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0.05
Vac/Vac Membrane 0.25 0.47 0.12 0.09 0.08 0.13 0.1 0 0.05 0 0.07 0.07 0.09 0.21 0.29 – 0.4 0.27 0.32 0.31 0.32 0.34 0.36 0.46
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 2 2
Bud 0 0 0 1 1 1 1 2 0 1 0 0 0 0 0 – 0 0 1 0 0 0 5 8
Bud Neck 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 2
Bud Site 0 0 0 0 0 1 2 0 1 2 0 2 0 0 0 – 0 0 – – – – – –
Cell Periphery 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 – 0 1 0 0 0 0 0 0
Cytoplasm 9 21 0 10 7 12 20 22 11 17 12 16 30 77 100 – 26 26 8 11 1 7 18 17
Endoplasmic Reticulum 0 1 1 0 0 0 0 0 0 0 0 0 3 3 2 – 6 2 4 0 0 2 3 6
Endosome 12 1 0 0 0 0 0 0 0 0 0 0 0 2 6 – 4 2 2 2 2 3 3 11
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 – 0 0 1 1 1 0 0 1
Mitochondria 36 43 11 47 33 42 114 84 74 68 89 85 20 28 18 – 7 6 11 8 0 9 34 35
Nucleus 92 90 12 52 23 20 40 39 27 22 39 40 187 184 196 – 30 39 102 122 33 103 102 57
Nuclear Periphery 0 2 0 0 1 0 2 0 4 1 2 2 0 1 1 – 0 0 1 1 0 0 1 1
Nucleolus 0 0 0 0 0 0 0 2 0 1 0 2 0 0 0 – 0 0 0 0 0 0 0 1
Peroxisomes 2 0 0 0 0 0 0 0 0 0 0 0 0 1 0 – 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 1 0 0 0 1 0 0 1 1 2 1 1 – 1 0 5 2 0 2 3 14
Vac/Vac Membrane 41 90 3 8 4 8 15 4 4 3 7 8 22 71 115 – 40 23 68 70 19 69 104 153
Unique Cell Count 165 192 24 89 53 63 143 111 88 88 106 113 248 341 403 99 86 215 225 61 203 290 330
Labelled Cell Count 193 248 28 119 69 84 194 154 121 115 150 156 265 368 439 114 99 215 225 61 203 290 330


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.2 4.2 3.8 3.8 3.9 3.7 3.2 3.7 3.5 3.3 3.4 3.4 5.0 5.1 4.7 4.4 5.4 5.4 4.9 5.0 5.5
Std Deviation (1e-4) 1.0 0.8 0.9 0.7 1.1 1.3 1.3 1.5 1.1 1.4 1.3 1.8 1.0 4.9 1.2 0.8 1.7 0.8 1.4 1.7 2.0
Intensity Change (Log2) – – – -0.01 0.04 -0.05 -0.25 -0.07 -0.15 -0.21 -0.17 -0.17 0.37 0.41 0.31 0.21 0.5 0.49 0.34 0.38 0.53


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – 0 0
Bud Neck – – – – – – – – – – – – – 0 0
Bud Site – – – – – – – – – – – – – 0 0
Cell Periphery – – – – – – – – – – – – – 0 0
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – 0 0
Endosome – – – – – – – – – – – – – 0 0
Golgi – – – – – – – – – – – – – 0 0
Mitochondria – – – – – – – – – – – – – 0 0
Nuclear Periphery – – – – – – – – – – – – – 0 0
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – 0 0
Peroxisomes – – – – – – – – – – – – – 0 0
SpindlePole – – – – – – – – – – – – – 0 0
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – 0 0
Cytoplasm – – – – – – – – – – – – – 0 0
Nucleus – – – – – – – – – – – – – 0 0
Vacuole – – – – – – – – – – – – – 2.6 1.5

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 1.9816 2.6974 2.1566 2.4569 2.2592 2.1816 2.034 1.2561 1.4762 0.9864 1.0838 1.634 3.2922 3.3261 3.6479 3.2772 2.7827 3.241
Actin 0.0128 0.0004 0.0022 0.0246 0.034 0.0003 0.0576 0.0009 0.0092 0.0003 0.0048 0.0091 0.0251 0.0002 0.0175 0.0001 0.0337 0.0031
Bud 0.0004 0.0002 0.0004 0.0032 0.0024 0.0001 0.0025 0.0005 0.0006 0.0001 0.0023 0.0003 0.0016 0.0002 0.0003 0.0001 0.0001 0.0002
Bud Neck 0.0057 0.0005 0.0003 0.0084 0.0035 0.0024 0.0053 0.0007 0.0005 0.0004 0.0034 0.0023 0.0097 0.0003 0.0014 0.0003 0.0009 0.0035
Bud Periphery 0.0012 0.0002 0.0005 0.005 0.0041 0.0002 0.0041 0.0003 0.0011 0 0.0031 0.0004 0.0032 0.0002 0.0003 0 0.0002 0.0003
Bud Site 0.0005 0.0007 0.0029 0.0043 0.0051 0.0001 0.0094 0.0027 0.0016 0.0001 0.0202 0.0005 0.0034 0.0008 0.0022 0.0002 0.0002 0.0003
Cell Periphery 0.0002 0.0001 0.0001 0.0004 0.0002 0 0.0016 0.0002 0.0002 0 0.0005 0.0001 0.0012 0.0001 0.0001 0 0.0001 0.0001
Cytoplasm 0.02 0.0143 0.0186 0.0043 0.0118 0.0068 0.0131 0.0784 0.0253 0.0791 0.0041 0.0257 0.0095 0.0513 0.037 0.0499 0.0013 0.0221
Cytoplasmic Foci 0.0203 0.0012 0.0037 0.0024 0.011 0.0001 0.0979 0.0017 0.0405 0.0004 0.0092 0.0039 0.0039 0.0012 0.0066 0.0003 0.0008 0.0005
Eisosomes 0.0005 0 0 0.0004 0.0001 0 0.0016 0 0.0001 0 0.0002 0.0001 0.0023 0 0.0001 0 0.0003 0
Endoplasmic Reticulum 0.0056 0.006 0.0011 0.0014 0.0022 0.0008 0.0068 0.0072 0.0028 0.0016 0.0008 0.0035 0.0089 0.0037 0.0036 0.0027 0.0004 0.002
Endosome 0.0172 0.0122 0.0023 0.0086 0.0096 0.0008 0.0985 0.0141 0.0291 0.0015 0.0306 0.0145 0.0229 0.0036 0.0091 0.0016 0.0022 0.0036
Golgi 0.0024 0.0005 0.0007 0.0049 0.007 0 0.0612 0.0003 0.0102 0 0.0615 0.0112 0.0047 0 0.0047 0 0.0034 0.0015
Lipid Particles 0.007 0.0005 0.005 0.0039 0.0108 0 0.0419 0.0002 0.0196 0 0.0134 0.0015 0.0087 0.0001 0.003 0 0.0036 0.001
Mitochondria 0.0037 0.0016 0.0014 0.0178 0.0067 0.0004 0.0676 0.0067 0.0424 0.0013 0.199 0.0114 0.0075 0.0012 0.0027 0.0008 0.0017 0.006
None 0.0163 0.002 0.019 0.0011 0.0127 0.0004 0.0173 0.0028 0.0674 0.0386 0.0006 0.0582 0.0025 0.0109 0.0021 0.0018 0.0009 0.0023
Nuclear Periphery 0.036 0.0306 0.0208 0.0133 0.0251 0.0074 0.0098 0.0397 0.0305 0.029 0.004 0.0154 0.04 0.0312 0.014 0.0151 0.0031 0.015
Nucleolus 0.0084 0.0041 0.004 0.0066 0.0088 0.0098 0.0042 0.0049 0.0066 0.0035 0.0319 0.0072 0.0066 0.0041 0.0055 0.0025 0.0081 0.0069
Nucleus 0.8021 0.9169 0.8993 0.8766 0.8076 0.9658 0.418 0.8198 0.6871 0.835 0.5903 0.809 0.8164 0.8543 0.8663 0.9209 0.9348 0.9136
Peroxisomes 0.0032 0.0001 0.0047 0.0023 0.0087 0 0.0571 0.0003 0.0121 0 0.0111 0.0087 0.0044 0.0001 0.0145 0 0.0019 0.0003
Punctate Nuclear 0.0301 0.0032 0.0113 0.0055 0.0257 0.0032 0.0112 0.003 0.007 0.0073 0.0026 0.0139 0.0048 0.0273 0.0054 0.0026 0.0015 0.0133
Vacuole 0.0041 0.0034 0.0012 0.0038 0.0019 0.0011 0.0077 0.0126 0.0039 0.001 0.0046 0.0023 0.0097 0.0074 0.003 0.0008 0.0005 0.0031
Vacuole Periphery 0.0024 0.0014 0.0005 0.0013 0.001 0.0003 0.0056 0.003 0.002 0.0005 0.0018 0.0008 0.0032 0.0017 0.0005 0.0003 0.0003 0.0009

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 13.5046 6.3912 14.7546 12.5966 13.3895 11.1153 11.3087 14.0538 12.6482 13.8104
Translational Efficiency 0.6427 1.5471 0.7565 1.0426 0.8918 0.797 0.8898 0.6959 0.8474 0.8399

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
103 371 2026 105 824 1258 488 276 927 1629 2514 381

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 676.72 711.18 886.11 918.64 733.44 761.03 1000.27 885.73 727.14 749.68 908.27 894.80
Standard Deviation 80.97 102.04 100.94 109.03 88.07 105.45 104.42 118.70 89.11 106.75 111.20 117.04
Intensity Change Log 2 — 0.071656 0.388927 0.440941 — 0.053274 0.447639 0.272188 — 0.062125 0.419761 0.355636

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000048 0.001231 0.000173 0.000884 0.000104 0.000733 0.000583 0.000772 0.000098 0.000847 0.000252 0.000803
Bud Neck 0.001671 0.019893 0.005140 0.004942 0.001683 0.019908 0.004343 0.012004 0.001681 0.019905 0.004985 0.010058
Bud Site 0.000252 0.009451 0.001300 0.003728 0.001249 0.006776 0.003151 0.025951 0.001138 0.007386 0.001660 0.019827
Cell Periphery 0.000182 0.000272 0.000070 0.000181 0.000124 0.000186 0.000106 0.000184 0.000130 0.000205 0.000077 0.000183
Cytoplasm 0.172752* 0.086978 0.019670 0.240943* 0.160481* 0.087275 0.075679 0.245747* 0.161844* 0.087207 0.030542 0.244423*
Cytoplasmic Foci 0.079825 0.034727 0.000122 0.005984 0.023217 0.037707 0.001163 0.013993 0.029507 0.037029 0.000324 0.011786
Eisosomes 0.000112 0.000090 0.000011 0.000062 0.000032 0.000076 0.000037 0.000049 0.000041 0.000079 0.000016 0.000052
Endoplasmic Reticulum 0.000772 0.009331 0.003879 0.009559 0.001408 0.005805 0.007945 0.007010 0.001337 0.006608 0.004668 0.007712
Endosome 0.001857 0.004864 0.000269 0.002546 0.000818 0.003221 0.001086 0.014138 0.000934 0.003595 0.000428 0.010944
Golgi 0.000988 0.001360 0.000020 0.000106 0.000111 0.001872 0.000057 0.008763 0.000208 0.001755 0.000027 0.006378
Lipid Particles 0.006881 0.009576 0.000128 0.001783 0.002337 0.007409 0.000856 0.001673 0.002842 0.007902 0.000270 0.001703
Mitochondria 0.001392 0.008588 0.000679 0.000604 0.000737 0.015838 0.000632 0.002877 0.000810 0.014187 0.000670 0.002250
Mitotic Spindle 0.006978 0.007837 0.000618 0.007081 0.000258 0.012004 0.000976 0.014820 0.001004 0.011055 0.000687 0.012687
None 0.007345 0.021450 0.002257 0.009715 0.009026 0.006718 0.003913 0.007591 0.008840 0.010073 0.002579 0.008176
Nuclear Periphery 0.000684 0.001543 0.001074 0.002099 0.000810 0.002821 0.001762 0.002479 0.000796 0.002530 0.001207 0.002374
Nuclear Periphery Foci 0.000359 0.002072 0.000083 0.005392 0.000346 0.000748 0.000832 0.001958 0.000348 0.001049 0.000228 0.002904
Nucleolus 0.005867 0.016378 0.000638 0.000855 0.002287 0.006747 0.000804 0.000944 0.002685 0.008941 0.000670 0.000920
Nucleus 0.704190* 0.726141* 0.960843* 0.579608* 0.789228* 0.751035* 0.866610* 0.582208* 0.779779* 0.745365* 0.942551* 0.581492*
Peroxisomes 0.002057 0.016259 0.000044 0.000251 0.001103 0.016459 0.000157 0.000994 0.001209 0.016414 0.000066 0.000789
Vacuole 0.005238 0.019413 0.002920 0.123524 0.004401 0.015712 0.029196 0.054137 0.004494 0.016555 0.008020 0.073259
Vacuole Periphery 0.000551 0.002545 0.000064 0.000152 0.000240 0.000950 0.000112 0.001709 0.000275 0.001314 0.000074 0.001280

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -5.21 -5.12 -7.04 0.19 -6.15 -7.19 -3.02 -7.64 -1.09 0.26 -8.77 -4.30 -10.06 -0.95 -6.08
Bud Neck -5.19 -5.20 -5.71 2.96 -2.34 -10.75 -4.48 -4.27 2.10 -2.67 -11.84 -6.85 -5.18 3.13 -3.05
Bud Site -3.83 -3.10 -5.43 0.32 -4.56 -5.21 -1.80 -3.39 -2.13 -2.50 -6.14 -1.27 -3.98 -2.04 -3.73
Cell Periphery -2.01 3.95 -0.87 1.45 -6.61 -2.93 0.04 -1.43 1.82 -1.84 -3.81 3.50 -2.17 2.14 -7.70
Cytoplasm 3.99 7.79 1.15 -2.71 -6.66 8.46 11.30 -0.36 -5.36 -7.11 9.35 19.07 0.19 -6.01 -11.59
Cytoplasmic Foci 3.27 6.59 5.99 7.04 -4.71 -4.40 10.41 4.03 7.95 -4.29 -2.61 12.32 6.79 10.18 -5.59
Eisosomes 0.56 3.98 0.04 -0.99 -8.65 -9.24 -4.81 -8.21 -1.32 -3.95 -8.06 5.41 -8.10 -1.76 -12.66
Endoplasmic Reticulum -6.05 -12.11 -8.69 -2.00 -6.43 -11.30 -14.97 -11.14 -5.35 1.15 -11.76 -20.32 -13.92 -5.67 -7.64
Endosome -2.84 2.42 -2.87 0.44 -6.19 -6.41 -3.40 -2.86 -1.72 -2.54 -7.32 2.62 -3.37 -1.66 -3.62
Golgi -0.70 1.74 1.43 4.12 -5.48 -3.61 2.26 -1.40 -0.51 -1.42 -4.03 2.90 -1.34 -0.27 -1.47
Lipid Particles -1.44 3.95 2.29 4.23 -8.00 -4.66 3.76 0.31 4.82 -4.01 -5.45 7.73 1.16 6.15 -10.41
Mitochondria -5.18 1.55 1.41 5.87 -0.44 -9.33 -0.03 -1.65 8.39 -1.66 -10.38 0.73 -1.47 9.56 -1.69
Mitotic Spindle -0.27 0.88 -0.95 -0.91 -1.72 -5.46 -1.66 -3.46 -1.07 -2.83 -5.38 -0.55 -3.68 -1.36 -3.61
None -2.91 3.17 -0.20 2.57 -2.31 1.27 4.24 1.21 -0.10 -3.09 -0.51 5.17 1.03 1.51 -4.29
Nuclear Periphery -2.95 -3.22 -6.86 -4.38 -5.98 -7.76 -10.22 -7.35 -2.38 -2.37 -8.06 -6.33 -9.21 -3.62 -7.17
Nuclear Periphery Foci -3.82 1.18 -2.42 -2.09 -2.45 -3.39 -4.71 -3.89 -3.44 -2.79 -5.18 -0.34 -3.80 -3.35 -3.78
Nucleolus -2.99 1.87 1.49 5.60 -1.59 -6.29 4.68 -0.01 4.60 -1.93 -7.31 5.28 0.84 7.20 -2.48
Nucleus -0.34 -9.33 4.16 5.20 13.65 3.66 -2.73 10.40 8.39 11.79 3.70 -17.43 12.00 9.96 22.13
Peroxisomes -4.37 3.30 2.70 4.91 -5.57 -8.19 3.39 0.50 8.28 -2.42 -9.34 4.71 1.35 9.65 -3.31
Vacuole -4.46 0.61 -8.15 -7.51 -8.20 -9.29 -10.11 -11.61 -10.61 -5.94 -10.32 -8.60 -14.13 -12.96 -13.00
Vacuole Periphery -2.14 1.45 1.11 2.78 -5.03 -6.06 1.95 -1.29 0.79 -1.65 -4.81 3.12 -1.06 2.38 -1.92
Protein Complexes26S Proteasome complex37S mitochondrial small ribosomal subunit54S mitochondrial large ribosomal subunit60S cytosolic large ribosomal subunitAar2-U5 snRNPAnaphase-Promoting Complex variant 1Anaphase-Promoting Complex variant 2Anaphase-Promoting Complex variant 3Anaphase-Promoting Complex variant 4Arp2/3 complexATG1 kinase complexBLOC-1 complexCcr4-Not complexCentral kinetochore CTF19 complexCleavage and polyadenylation specificity factorcomplexCMG helicase complexCMG-Pol epsilon complexCOMPASS complexCOP9 signalosome COPI vesicle coat complexCoQ6 biosynthesis complexCore mediator complexCORVET complexCTF18-RFC complexCURI complex variant 1CURI complex variant 2CURI complex variant 3Cytoplasmic exosome complex, DIS3 variantDASH complexDNA-directed RNA Polymerase I complexDNA-directed RNA polymerase II complexDNA-directed RNA polymerase III complexDNA replication factor C complexEGO complexELG1-RFC complex Elongator holoenzyme complexEndoplasmic Reticulum Membrane ComplexEukaryotic translation initiation factor 2BcomplexEukaryotic translation initiation factor 3core complexExocystExomer complexFAR complexGeneral transcription factor complex TFIIDGeneral transcription factor complex TFIIHGET4-GET5 transmembrane domain recognition complexGID ubiquitin ligase complexGin4 complexGolgi transport complexGSE complexHMC complexHOPS complexINO80 chromatin remodeling complexLSM1-7 complexLsm1-7-Pat1 complexLSM2-7 complexLSM2-8 complexMCM complexMICOS complexMitochondrial electron transport complex IIIMitochondrial inner membrane pre-sequence translocasecomplexMitochondrial outer membrane translocase holocomplexMitochondrial proton-transporting ATP synthase complexMitochondrial pyruvate dehydrogenase complexNineTeen complexNOP8 60s ribosome pre-assembly complexNuA3 histone acetyltransferase complexNuA4 histone acetyltransferase complexNuclear condensin complexNuclear/nucleolar exosome complex, DIS3-RRP6 variantNuclear pore complexNucleolar ribonuclease MRP complexNucleolar ribonuclease P complexOligosaccharyl transferase complex variant 1Oligosaccharyl transferase complex variant 2PAF1 complexPAS complexPhosphatidylinositol 3-kinase complex IPolarisomePrefoldin complexPRP19-associated complexRad17 RFC-like complexRetromer complexRPD3L histone deacetylase complexRPD3S histone deacetylase complexRQC complexRSC complex variant RSC1RSC complex variant RSC2SAGA complexSCF-Met30 ubiquitin ligase complexSCF-Ufo1 ubiquitin ligase complexSEA complexSeptin complexSET3C histone deacetylase complexSignal recognition particleSLIK (SAGA-like) complexSmall ribosomal subunit processome, variant1Small ribosomal subunit processome, variant2Small ribosomal subunit processome, variant3SMC5-SMC6 SUMO ligase complexSm complexSpliceosomal commitment complexSPOTS complexSWI/SNF chromatin remodelling complexSwr1 chromatin remodelling complexTHO complexTranscription factor TFIIIC complexTranslocon complexTRAPPIII protein complexTRAPPII protein complexTRAPPI protein complexTREX complexU1 snRNPU2 snRNPU4 snRNPU4/U6 snRNPU4/U6.U5 tri-snRNP complexU5 snRNPU6 snRNP complexUTP-A complexUTP-B complexUTP-C complex variant 1UTP-C complex variant 2UTP-C complex variant 3Vacuolar proton translocating ATPase complex,Golgi variantVacuolar proton translocating ATPase complex,vacuole variantCellular Components••••••••••••••••actinbud neckcell peripherycytoplasmendosomeERGolgimitochondrionmixednuclear peripherynucleolusnucleusperoxisomespindle polevacuolar membranevacuole
Standard name
Human Ortholog
Description Mitochondrial tRNA:pseudouridine synthase; catalyzes the formation of pseudouridine at position 32 in mitochondrial tRNAs; contains an N-terminal mitochondrial targeting sequence; PUS9 has a paralog, RIB2, that arose from the whole genome duplication
Localization
Cell Percentages nucleus (34%), cytoplasm (9%), mixed (52%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Pus9

Pus9


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Pus9-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available