Standard name | |
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Human Ortholog | |
Description | Mitochondrial tRNA:pseudouridine synthase; catalyzes the formation of pseudouridine at position 32 in mitochondrial tRNAs; contains an N-terminal mitochondrial targeting sequence; PUS9 has a paralog, RIB2, that arose from the whole genome duplication |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 2 |
Bud | 0 | 0 | 0 | 1 | 1 | 1 | 1 | 2 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 1 | 0 | 0 | 0 | 5 | 8 |
Bud Neck | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 1 | 2 | 0 | 1 | 2 | 0 | 2 | 0 | 0 | 0 | – | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 9 | 21 | 0 | 10 | 7 | 12 | 20 | 22 | 11 | 17 | 12 | 16 | 30 | 77 | 100 | – | 26 | 26 | 8 | 11 | 1 | 7 | 18 | 17 |
Endoplasmic Reticulum | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3 | 3 | 2 | – | 6 | 2 | 4 | 0 | 0 | 2 | 3 | 6 |
Endosome | 12 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 6 | – | 4 | 2 | 2 | 2 | 2 | 3 | 3 | 11 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | – | 0 | 0 | 1 | 1 | 1 | 0 | 0 | 1 |
Mitochondria | 36 | 43 | 11 | 47 | 33 | 42 | 114 | 84 | 74 | 68 | 89 | 85 | 20 | 28 | 18 | – | 7 | 6 | 11 | 8 | 0 | 9 | 34 | 35 |
Nucleus | 92 | 90 | 12 | 52 | 23 | 20 | 40 | 39 | 27 | 22 | 39 | 40 | 187 | 184 | 196 | – | 30 | 39 | 102 | 122 | 33 | 103 | 102 | 57 |
Nuclear Periphery | 0 | 2 | 0 | 0 | 1 | 0 | 2 | 0 | 4 | 1 | 2 | 2 | 0 | 1 | 1 | – | 0 | 0 | 1 | 1 | 0 | 0 | 1 | 1 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 1 | 0 | 2 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 |
Peroxisomes | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 1 | 2 | 1 | 1 | – | 1 | 0 | 5 | 2 | 0 | 2 | 3 | 14 |
Vac/Vac Membrane | 41 | 90 | 3 | 8 | 4 | 8 | 15 | 4 | 4 | 3 | 7 | 8 | 22 | 71 | 115 | – | 40 | 23 | 68 | 70 | 19 | 69 | 104 | 153 |
Unique Cell Count | 165 | 192 | 24 | 89 | 53 | 63 | 143 | 111 | 88 | 88 | 106 | 113 | 248 | 341 | 403 | 99 | 86 | 215 | 225 | 61 | 203 | 290 | 330 | |
Labelled Cell Count | 193 | 248 | 28 | 119 | 69 | 84 | 194 | 154 | 121 | 115 | 150 | 156 | 265 | 368 | 439 | 114 | 99 | 215 | 225 | 61 | 203 | 290 | 330 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 4.2 | 4.2 | 3.8 | 3.8 | 3.9 | 3.7 | 3.2 | 3.7 | 3.5 | 3.3 | 3.4 | 3.4 | 5.0 | 5.1 | 4.7 | 4.4 | 5.4 | 5.4 | 4.9 | 5.0 | 5.5 |
Std Deviation (1e-4) | 1.0 | 0.8 | 0.9 | 0.7 | 1.1 | 1.3 | 1.3 | 1.5 | 1.1 | 1.4 | 1.3 | 1.8 | 1.0 | 4.9 | 1.2 | 0.8 | 1.7 | 0.8 | 1.4 | 1.7 | 2.0 |
Intensity Change (Log2) | – | – | – | -0.01 | 0.04 | -0.05 | -0.25 | -0.07 | -0.15 | -0.21 | -0.17 | -0.17 | 0.37 | 0.41 | 0.31 | 0.21 | 0.5 | 0.49 | 0.34 | 0.38 | 0.53 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Actin | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 |
Bud Neck | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 |
Bud Site | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 |
Cell Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 |
Cyto | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endoplasmic Reticulum | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 |
Endosome | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 |
Golgi | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 |
Mitochondria | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 |
Nuclear Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 |
Nuc | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleolus | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 |
Peroxisomes | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 |
SpindlePole | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 |
Vac | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cortical Patches | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 |
Cytoplasm | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 |
Nucleus | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 |
Vacuole | – | – | – | – | – | – | – | – | – | – | – | – | – | 2.6 | 1.5 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 1.9816 | 2.6974 | 2.1566 | 2.4569 | 2.2592 | 2.1816 | 2.034 | 1.2561 | 1.4762 | 0.9864 | 1.0838 | 1.634 | 3.2922 | 3.3261 | 3.6479 | 3.2772 | 2.7827 | 3.241 |
Actin | 0.0128 | 0.0004 | 0.0022 | 0.0246 | 0.034 | 0.0003 | 0.0576 | 0.0009 | 0.0092 | 0.0003 | 0.0048 | 0.0091 | 0.0251 | 0.0002 | 0.0175 | 0.0001 | 0.0337 | 0.0031 |
Bud | 0.0004 | 0.0002 | 0.0004 | 0.0032 | 0.0024 | 0.0001 | 0.0025 | 0.0005 | 0.0006 | 0.0001 | 0.0023 | 0.0003 | 0.0016 | 0.0002 | 0.0003 | 0.0001 | 0.0001 | 0.0002 |
Bud Neck | 0.0057 | 0.0005 | 0.0003 | 0.0084 | 0.0035 | 0.0024 | 0.0053 | 0.0007 | 0.0005 | 0.0004 | 0.0034 | 0.0023 | 0.0097 | 0.0003 | 0.0014 | 0.0003 | 0.0009 | 0.0035 |
Bud Periphery | 0.0012 | 0.0002 | 0.0005 | 0.005 | 0.0041 | 0.0002 | 0.0041 | 0.0003 | 0.0011 | 0 | 0.0031 | 0.0004 | 0.0032 | 0.0002 | 0.0003 | 0 | 0.0002 | 0.0003 |
Bud Site | 0.0005 | 0.0007 | 0.0029 | 0.0043 | 0.0051 | 0.0001 | 0.0094 | 0.0027 | 0.0016 | 0.0001 | 0.0202 | 0.0005 | 0.0034 | 0.0008 | 0.0022 | 0.0002 | 0.0002 | 0.0003 |
Cell Periphery | 0.0002 | 0.0001 | 0.0001 | 0.0004 | 0.0002 | 0 | 0.0016 | 0.0002 | 0.0002 | 0 | 0.0005 | 0.0001 | 0.0012 | 0.0001 | 0.0001 | 0 | 0.0001 | 0.0001 |
Cytoplasm | 0.02 | 0.0143 | 0.0186 | 0.0043 | 0.0118 | 0.0068 | 0.0131 | 0.0784 | 0.0253 | 0.0791 | 0.0041 | 0.0257 | 0.0095 | 0.0513 | 0.037 | 0.0499 | 0.0013 | 0.0221 |
Cytoplasmic Foci | 0.0203 | 0.0012 | 0.0037 | 0.0024 | 0.011 | 0.0001 | 0.0979 | 0.0017 | 0.0405 | 0.0004 | 0.0092 | 0.0039 | 0.0039 | 0.0012 | 0.0066 | 0.0003 | 0.0008 | 0.0005 |
Eisosomes | 0.0005 | 0 | 0 | 0.0004 | 0.0001 | 0 | 0.0016 | 0 | 0.0001 | 0 | 0.0002 | 0.0001 | 0.0023 | 0 | 0.0001 | 0 | 0.0003 | 0 |
Endoplasmic Reticulum | 0.0056 | 0.006 | 0.0011 | 0.0014 | 0.0022 | 0.0008 | 0.0068 | 0.0072 | 0.0028 | 0.0016 | 0.0008 | 0.0035 | 0.0089 | 0.0037 | 0.0036 | 0.0027 | 0.0004 | 0.002 |
Endosome | 0.0172 | 0.0122 | 0.0023 | 0.0086 | 0.0096 | 0.0008 | 0.0985 | 0.0141 | 0.0291 | 0.0015 | 0.0306 | 0.0145 | 0.0229 | 0.0036 | 0.0091 | 0.0016 | 0.0022 | 0.0036 |
Golgi | 0.0024 | 0.0005 | 0.0007 | 0.0049 | 0.007 | 0 | 0.0612 | 0.0003 | 0.0102 | 0 | 0.0615 | 0.0112 | 0.0047 | 0 | 0.0047 | 0 | 0.0034 | 0.0015 |
Lipid Particles | 0.007 | 0.0005 | 0.005 | 0.0039 | 0.0108 | 0 | 0.0419 | 0.0002 | 0.0196 | 0 | 0.0134 | 0.0015 | 0.0087 | 0.0001 | 0.003 | 0 | 0.0036 | 0.001 |
Mitochondria | 0.0037 | 0.0016 | 0.0014 | 0.0178 | 0.0067 | 0.0004 | 0.0676 | 0.0067 | 0.0424 | 0.0013 | 0.199 | 0.0114 | 0.0075 | 0.0012 | 0.0027 | 0.0008 | 0.0017 | 0.006 |
None | 0.0163 | 0.002 | 0.019 | 0.0011 | 0.0127 | 0.0004 | 0.0173 | 0.0028 | 0.0674 | 0.0386 | 0.0006 | 0.0582 | 0.0025 | 0.0109 | 0.0021 | 0.0018 | 0.0009 | 0.0023 |
Nuclear Periphery | 0.036 | 0.0306 | 0.0208 | 0.0133 | 0.0251 | 0.0074 | 0.0098 | 0.0397 | 0.0305 | 0.029 | 0.004 | 0.0154 | 0.04 | 0.0312 | 0.014 | 0.0151 | 0.0031 | 0.015 |
Nucleolus | 0.0084 | 0.0041 | 0.004 | 0.0066 | 0.0088 | 0.0098 | 0.0042 | 0.0049 | 0.0066 | 0.0035 | 0.0319 | 0.0072 | 0.0066 | 0.0041 | 0.0055 | 0.0025 | 0.0081 | 0.0069 |
Nucleus | 0.8021 | 0.9169 | 0.8993 | 0.8766 | 0.8076 | 0.9658 | 0.418 | 0.8198 | 0.6871 | 0.835 | 0.5903 | 0.809 | 0.8164 | 0.8543 | 0.8663 | 0.9209 | 0.9348 | 0.9136 |
Peroxisomes | 0.0032 | 0.0001 | 0.0047 | 0.0023 | 0.0087 | 0 | 0.0571 | 0.0003 | 0.0121 | 0 | 0.0111 | 0.0087 | 0.0044 | 0.0001 | 0.0145 | 0 | 0.0019 | 0.0003 |
Punctate Nuclear | 0.0301 | 0.0032 | 0.0113 | 0.0055 | 0.0257 | 0.0032 | 0.0112 | 0.003 | 0.007 | 0.0073 | 0.0026 | 0.0139 | 0.0048 | 0.0273 | 0.0054 | 0.0026 | 0.0015 | 0.0133 |
Vacuole | 0.0041 | 0.0034 | 0.0012 | 0.0038 | 0.0019 | 0.0011 | 0.0077 | 0.0126 | 0.0039 | 0.001 | 0.0046 | 0.0023 | 0.0097 | 0.0074 | 0.003 | 0.0008 | 0.0005 | 0.0031 |
Vacuole Periphery | 0.0024 | 0.0014 | 0.0005 | 0.0013 | 0.001 | 0.0003 | 0.0056 | 0.003 | 0.002 | 0.0005 | 0.0018 | 0.0008 | 0.0032 | 0.0017 | 0.0005 | 0.0003 | 0.0003 | 0.0009 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 13.5046 | 6.3912 | 14.7546 | 12.5966 | 13.3895 | 11.1153 | 11.3087 | 14.0538 | 12.6482 | 13.8104 |
Translational Efficiency | 0.6427 | 1.5471 | 0.7565 | 1.0426 | 0.8918 | 0.797 | 0.8898 | 0.6959 | 0.8474 | 0.8399 |
Dataset | Hit |
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Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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