Standard name
Human Ortholog
Description Mitochondrial protein that interacts with mitochondrial RNA polymerase; interacts with an N-terminal region of mitochondrial RNA polymerase (Rpo41p) and couples RNA processing and translation to transcription
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0.05 0 0 0 0 0 0 0 0 0 0 0.08 0.05 0 0.12 0.09 0.06 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.06 0.05 0.09 0.11 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.05 0.05 0.06 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0.05 0 0 0 0 0.08 0.21 0.14 0.1 0.16 0.18 0 0.08 0.06 0.06 0.06 0.05
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0.21 0.14 0.19 0.19 0.18 0.12 0 0.06 0 0 0.07 0.09
Mitochondria 0.98 0.89 0.91 0.96 0.9 0.91 0.91 0.82 0.94 0.94 0.97 0.96 0.76 0.55 0.48 0.13 0.1 0.11 0.81 0.75 0.74 0.74 0.73 0.69
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0.06 0 0 0 0 0.08 0.07 0.09 0.09 0 0.06 0.08 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0.07 0.12 0.08 0.06 0.09 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0.11 0.05 0 0 0 0 0 0 0 0 0.08 0.24 0.07 0.15 0.21 0.15 0.07 0 0.13 0.05 0 0
SpindlePole 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0.07 0.06 0.11 0.12 0.13 0 0 0 0 0 0
Vac/Vac Membrane 0.08 0.13 0 0 0.06 0 0 0.06 0 0 0.05 0 0.08 0.14 0.14 0.2 0.19 0.19 0 0 0 0.06 0.05 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 1 2 0 0 0 1 1 0 0 0 0 8 5 10 4 2 8 3 3 6 2 8 13
Bud 5 4 21 24 16 23 34 16 28 29 30 30 4 4 6 17 10 14 0 0 1 0 1 2
Bud Neck 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 1 0 1 3 3
Bud Site 1 1 1 1 3 10 16 10 12 12 15 10 0 0 0 2 1 1 – – – – – –
Cell Periphery 7 14 25 19 7 5 3 6 3 7 9 9 20 19 14 34 26 18 0 0 0 0 0 2
Cytoplasm 1 2 6 10 23 19 17 27 8 8 6 7 7 20 22 15 26 34 0 0 0 1 1 1
Endoplasmic Reticulum 4 4 0 5 5 3 0 1 0 3 0 3 7 8 17 14 15 18 0 1 1 0 1 0
Endosome 1 7 20 11 24 6 4 48 4 2 0 3 20 76 49 29 48 56 15 20 35 15 25 23
Golgi 1 8 4 0 1 1 0 0 0 0 0 0 55 53 66 53 54 38 12 15 22 9 30 47
Mitochondria 359 229 561 519 634 584 853 798 873 1089 714 986 198 202 170 38 29 34 292 203 471 184 324 351
Nucleus 2 1 2 0 4 18 25 19 13 20 2 7 2 4 0 6 8 12 0 0 0 1 2 1
Nuclear Periphery 22 9 21 17 18 49 65 87 86 48 46 78 2 2 2 9 5 6 3 2 3 2 1 3
Nucleolus 15 4 11 5 30 42 109 80 52 99 39 44 0 1 0 3 1 6 1 0 1 1 0 3
Peroxisomes 5 4 68 27 25 8 6 30 17 8 8 13 21 88 26 44 64 48 26 9 81 12 13 22
SpindlePole 3 3 17 17 12 14 28 45 30 18 14 12 2 26 20 32 37 42 0 0 1 0 1 2
Vac/Vac Membrane 30 34 23 10 41 21 14 56 36 35 34 38 22 51 49 56 57 59 3 11 1 15 22 22
Unique Cell Count 365 256 614 543 707 639 936 976 930 1155 738 1026 260 370 355 286 299 312 361 272 633 251 443 507
Labelled Cell Count 456 325 782 665 843 803 1176 1224 1162 1378 917 1240 368 559 451 356 383 394 361 272 633 251 443 507


Mitochondria

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.2 6.1 5.4 5.0 5.2 4.6 4.4 4.8 4.4 4.3 4.1 4.3 6.8 6.8 6.5 6.9 7.1 6.3 7.3 7.4 7.6
Std Deviation (1e-4) 0.8 1.4 0.9 1.3 1.4 1.6 1.2 1.2 1.1 1.1 1.0 1.1 1.0 1.2 1.1 1.2 1.4 1.3 1.1 1.2 1.4
Intensity Change (Log2) – – – -0.12 -0.05 -0.22 -0.28 -0.16 -0.29 -0.31 -0.38 -0.31 0.35 0.34 0.27 0.36 0.41 0.22 0.44 0.45 0.5


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0.9 -1.3 0 0 0 0 0 0 0 -1.5 -2.3 -1.6 1.8 -0.1 0.8
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery -0.5 -3.6 0 0 0 0 0 0 0 2.2 0.8 -0.1 4.4 2.9 1.2
Cytoplasm 1.3 2.8 2.5 1.3 2.4 -0.2 0 -0.3 0 0 4.2 4.7 3.9 6.0 7.0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 5.5 0 0 6.0
Endosome -1.3 0.1 0 0 0 0 0 0 0 2.9 8.9 6.2 4.2 6.9 7.7
Golgi 0 0 0 0 0 0 0 0 0 11.0 8.9 10.4 10.3 10.1 8.0
Mitochondria 2.9 -1.0 0 0 0 0 0 0 0 -6.1 -13.4 -15.1 -23.1 -24.2 -24.2
Nucleus 0 0 3.5 3.5 2.8 2.1 2.5 0 0 0 0 0 0 0 0
Nuclear Periphery -0.3 -0.9 0 0 0 0 0 0 0 -2.2 -2.9 -2.8 -0.2 -1.5 -1.3
Nucleolus -1.3 2.6 0 0 0 0 0 0 0 0 0 0 0 0 0.1
Peroxisomes -3.8 -5.3 0 0 0 0 0 0 0 -1.3 5.3 -1.9 1.8 4.2 1.9
SpindlePole 0.4 -1.3 0 0 0 0 0 0 0 -1.9 3.2 2.2 5.2 5.8 6.3
Vacuole -1.9 1.7 -0.4 -2.8 1.8 0.1 -0.8 0.8 0 2.9 5.8 5.8 7.8 7.7 7.7

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration – – – – – – – – – – – – – – – – – –
Actin – – – – – – 0.0367 0.0134 0.0237 0.0022 0.0141 0.0037 0.013 0.0199 0.0197 0.0048 0.007 0.0133
Bud – – – – – – 0.0005 0.0017 0.0009 0.0001 0.0012 0.0002 0.0004 0.0012 0.0027 0.0032 0.0003 0.0009
Bud Neck – – – – – – 0.0013 0.0006 0.0006 0.0001 0.0006 0.0003 0.0003 0.0014 0.0016 0.0006 0.0004 0.005
Bud Periphery – – – – – – 0.0008 0.0019 0.0012 0.0003 0.0019 0.0006 0.0008 0.0014 0.0038 0.0177 0.0007 0.0019
Bud Site – – – – – – 0.0043 0.0078 0.0029 0.0001 0.0022 0.0003 0.0009 0.027 0.0168 0.0024 0.0005 0.0019
Cell Periphery – – – – – – 0.0004 0.0004 0.0003 0.0001 0.0003 0.0002 0.0003 0.0006 0.0013 0.0036 0.0002 0.0002
Cytoplasm – – – – – – 0.0039 0.0036 0.011 0.0002 0.0011 0.0002 0.0019 0.0032 0.005 0.0017 0.0002 0.0085
Cytoplasmic Foci – – – – – – 0.026 0.0242 0.0191 0.0063 0.0102 0.0049 0.0204 0.0327 0.0281 0.0087 0.0126 0.0126
Eisosomes – – – – – – 0.0004 0.0007 0.0005 0.0001 0.0003 0.0002 0.0009 0.0019 0.0015 0.0003 0.0004 0.0002
Endoplasmic Reticulum – – – – – – 0.0007 0.0026 0.0004 0.0003 0.0023 0.0004 0.0013 0.0011 0.0017 0.0004 0.0002 0.0004
Endosome – – – – – – 0.0668 0.0722 0.0447 0.0822 0.0754 0.0286 0.0773 0.0872 0.0644 0.0311 0.0218 0.0261
Golgi – – – – – – 0.1046 0.0863 0.0614 0.0511 0.0592 0.0661 0.0508 0.0687 0.0386 0.0233 0.0437 0.0515
Lipid Particles – – – – – – 0.0607 0.0184 0.012 0.0034 0.0195 0.0193 0.077 0.033 0.0261 0.005 0.0295 0.0233
Mitochondria – – – – – – 0.6269 0.7209 0.7868 0.8354 0.7734 0.829 0.6228 0.5832 0.6872 0.7402 0.8307 0.7807
None – – – – – – 0.014 0.0019 0.0048 0 0.0006 0.0001 0.0469 0.0672 0.0306 0.1024 0.0015 0.0267
Nuclear Periphery – – – – – – 0.0008 0.0018 0.0003 0.0001 0.0064 0.0002 0.0074 0.001 0.0016 0.0003 0.0002 0.0011
Nucleolus – – – – – – 0.0059 0.0004 0.0003 0 0.0007 0.0002 0.0012 0.0026 0.0029 0.0014 0.0004 0.002
Nucleus – – – – – – 0.0007 0.0005 0.0002 0 0.0026 0.0002 0.002 0.0019 0.0017 0.001 0.0003 0.002
Peroxisomes – – – – – – 0.0292 0.0301 0.0164 0.0136 0.0085 0.0217 0.0587 0.0475 0.0423 0.0287 0.045 0.0318
Punctate Nuclear – – – – – – 0.0018 0.0002 0.0002 0 0.0009 0 0.0026 0.0009 0.0011 0.0015 0.0003 0.001
Vacuole – – – – – – 0.0054 0.005 0.0065 0.0018 0.0088 0.0076 0.0051 0.0094 0.0131 0.0126 0.0016 0.0052
Vacuole Periphery – – – – – – 0.0084 0.0054 0.0057 0.0027 0.0098 0.0159 0.0081 0.0074 0.0084 0.0091 0.0024 0.0035

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 5.0041 5.9645 7.7453 8.1673 7.5694 7.502 7.5384 10.9389 8.2331 10.287
Translational Efficiency 1.3543 0.6925 0.8583 1.1791 1.0698 1.2411 0.9251 0.78 1.2016 1.1418

Hit Data

Dataset Hit
Protein Concentration –
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
57 118 361 1275 836 2043 83 206 893 2161 444 1481

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 856.42 919.04 972.16 922.96 773.99 808.70 1026.52 997.06 779.25 814.73 982.32 933.27
Standard Deviation 113.47 121.15 109.45 132.29 107.40 114.29 120.70 134.39 109.66 117.38 113.63 135.04
Intensity Change Log 2 — 0.101809 0.182875 0.107950 — 0.063290 0.407375 0.365365 — 0.083651 0.293813 0.235887

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.005761 0.003281 0.022025 0.019565 0.002085 0.002654 0.008395 0.038643 0.002320 0.002688 0.019477 0.022218
Bud Neck 0.001559 0.006523 0.006461 0.003722 0.003358 0.000951 0.010306 0.008765 0.003243 0.001255 0.007180 0.004424
Bud Site 0.002881 0.012066 0.044095 0.055139 0.006423 0.004871 0.029086 0.082997 0.006197 0.005264 0.041289 0.059014
Cell Periphery 0.000228 0.000687 0.000855 0.000724 0.000452 0.000332 0.000325 0.001037 0.000438 0.000351 0.000756 0.000767
Cytoplasm 0.001951 0.001305 0.008351 0.012518 0.002225 0.001991 0.011647 0.010291 0.002207 0.001954 0.008967 0.012208
Cytoplasmic Foci 0.054465 0.047573 0.110727 0.065561 0.040938 0.029962 0.102047 0.091667 0.041801 0.030924 0.109104 0.069193
Eisosomes 0.000091 0.000093 0.000155 0.000141 0.000090 0.000069 0.000156 0.000212 0.000091 0.000070 0.000155 0.000151
Endoplasmic Reticulum 0.005473 0.000342 0.003845 0.001105 0.000352 0.000160 0.004564 0.001060 0.000679 0.000170 0.003980 0.001099
Endosome 0.051475 0.144893 0.110239 0.088643 0.073796 0.063080 0.133737 0.086743 0.072371 0.067547 0.114632 0.088378
Golgi 0.039643 0.082961 0.104309 0.130371* 0.073430 0.081206 0.070754 0.140719* 0.071273 0.081302 0.098037 0.131810*
Lipid Particles 0.026230 0.001120 0.002927 0.000996 0.010979 0.004145 0.012012 0.000592 0.011953 0.003980 0.004625 0.000940
Mitochondria 0.699865* 0.570956* 0.285656* 0.447139* 0.697802* 0.760335* 0.230261* 0.285195* 0.697934* 0.749994* 0.275300* 0.424614*
Mitotic Spindle 0.015995 0.013262 0.134948* 0.053484 0.008351 0.004318 0.110229* 0.066910 0.008839 0.004807 0.130327* 0.055351
None 0.000468 0.000156 0.000174 0.000210 0.000340 0.000684 0.000674 0.000157 0.000348 0.000656 0.000268 0.000202
Nuclear Periphery 0.001114 0.000075 0.000373 0.000275 0.000174 0.000053 0.000542 0.000144 0.000234 0.000054 0.000405 0.000257
Nuclear Periphery Foci 0.011679 0.000222 0.001405 0.000441 0.000428 0.000101 0.002197 0.000392 0.001146 0.000108 0.001553 0.000434
Nucleolus 0.001505 0.000428 0.000628 0.000428 0.000511 0.000066 0.003251 0.000676 0.000574 0.000086 0.001118 0.000462
Nucleus 0.002293 0.000641 0.002027 0.000977 0.000415 0.000437 0.016429 0.002432 0.000535 0.000449 0.004719 0.001179
Peroxisomes 0.064984 0.088704 0.112161* 0.052898 0.063763 0.031883 0.204180* 0.142157* 0.063841 0.034986 0.129363* 0.065314
Vacuole 0.008674 0.013421 0.035251 0.042551 0.006034 0.005476 0.034885 0.027953 0.006202 0.005910 0.035183 0.040520
Vacuole Periphery 0.003665 0.011292 0.013389 0.023114 0.008052 0.007224 0.014322 0.011256 0.007772 0.007446 0.013563 0.021465

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin 0.93 -3.50 -4.05 -6.15 0.54 -0.95 -2.44 -4.64 -4.57 -3.57 -0.63 -5.22 -8.39 -8.25 -0.65
Bud Neck -2.05 -5.75 -3.94 1.11 3.47 3.47 -2.44 -2.25 -3.32 0.36 3.00 -4.05 -1.71 -6.82 3.08
Bud Site -2.42 -7.22 -14.20 -8.50 -1.69 1.06 -2.80 -6.74 -6.90 -3.77 0.68 -7.10 -14.57 -15.19 -2.98
Cell Periphery -1.88 -1.39 -2.72 -0.11 0.27 0.79 1.11 -1.79 -2.09 -2.21 0.61 -0.84 -1.85 -2.12 -0.03
Cytoplasm 1.22 -3.81 -9.28 -10.56 -1.86 0.28 -1.42 -5.16 -5.55 0.36 0.33 -3.54 -9.04 -9.97 -1.22
Cytoplasmic Foci 0.50 -3.84 -0.84 -2.52 6.16 3.61 -5.34 -5.85 -7.41 0.82 3.65 -10.56 -7.18 -12.50 6.18
Eisosomes -0.05 -2.79 -2.71 -3.65 0.97 1.93 -2.60 -5.05 -6.63 -1.60 1.93 -3.78 -4.88 -10.88 0.52
Endoplasmic Reticulum 1.25 0.28 1.06 -4.80 3.95 4.24 -3.29 -3.38 -4.22 2.61 1.90 -4.99 -1.52 -7.45 4.62
Endosome -4.41 -4.41 -3.09 3.11 2.87 2.06 -3.42 -1.37 -2.82 2.53 0.95 -5.54 -2.92 -5.04 3.73
Golgi -2.97 -6.15 -9.89 -3.45 -3.19 -1.39 0.29 -4.81 -4.37 -4.04 -1.88 -3.36 -9.25 -8.70 -4.54
Lipid Particles 1.99 1.78 1.99 0.20 3.66 3.52 -0.33 5.64 4.97 2.82 4.01 3.07 5.77 4.45 4.40
Mitochondria 2.05 7.78 4.90 3.44 -8.17 -4.63 11.79 16.00 19.88 -1.26 -3.95 21.90 18.34 27.88 -8.24
Mitotic Spindle 0.44 -9.45 -5.38 -9.11 7.01 2.94 -4.21 -5.42 -5.85 1.62 2.99 -12.02 -12.87 -14.76 7.05
None 1.60 1.47 1.33 -2.15 -1.27 -0.93 -0.93 1.08 1.58 1.45 -0.88 0.39 0.98 1.46 1.08
Nuclear Periphery 1.61 1.09 1.29 -4.18 1.41 2.47 -1.90 0.21 -1.66 1.94 2.91 -2.13 -0.42 -5.02 2.06
Nuclear Periphery Foci 1.99 1.52 1.94 -2.16 1.87 2.37 -1.86 0.18 -1.35 1.88 2.65 -1.38 1.71 -3.95 2.19
Nucleolus 0.90 0.37 0.75 -0.40 0.65 2.30 -2.30 -0.71 -1.14 1.22 2.49 -1.53 -0.25 -1.85 1.24
Nucleus 1.03 -0.39 0.60 -0.96 1.60 -0.05 -1.48 -1.46 -1.45 1.04 0.39 -2.30 -1.82 -1.94 1.66
Peroxisomes -0.96 -2.18 0.62 2.17 5.40 5.72 -4.85 -4.68 -6.90 1.96 5.31 -5.82 -0.16 -6.48 5.93
Vacuole -0.88 -4.83 -7.36 -6.70 -0.74 0.43 -3.85 -3.81 -3.97 0.59 0.24 -7.17 -12.53 -13.66 -0.62
Vacuole Periphery -2.14 -4.04 -8.81 -3.14 -3.76 0.81 -1.26 -1.23 -1.59 0.55 0.33 -2.85 -7.89 -8.45 -3.36
Standard name
Human Ortholog
Description Mitochondrial protein that interacts with mitochondrial RNA polymerase; interacts with an N-terminal region of mitochondrial RNA polymerase (Rpo41p) and couples RNA processing and translation to transcription
Localization
Cell Percentages mitochondrion (94%)
Cell Cycle Regulation No
Subcompartmental Group mito-2

Mtf2

Mtf2


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Mtf2-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available