Standard name
Human Ortholog
Description Transcription factor; involved in regulation of cell cycle progression from G1 to S phase, forms a complex with Swi6p that binds to MluI cell cycle box regulatory element in promoters of DNA synthesis genes
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0.05 0.05 0.11 0.05 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0.05 0.05 0.05 0.05 0 0 0 0 0.1 0.1 0.12 0.11 0 0.12 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.08 0 0 0 0 0 0.05
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.11 0.16 0.05 0.05 0.05 0.24 0.29 0.17 0.5 0.57 0.56 0.29 0.05 0 0 0 0.05 0.2 0 0 0 0 0 0
Nucleus 0.94 0.88 0.95 0.94 0.89 0.84 0.82 0.83 0.78 0.74 0.8 0.79 0.86 0.85 0.8 0.77 0.86 0.72 0.83 0.83 0.81 0.57 0.55 0.49
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.08 0 0 0 0 0.06 0 0.05 0.12 0.12 0.21 0.12 0.09 0.05 0.08 0 0.12 0.08 0.08 0.06 0.06 0.21 0.19 0.17
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0.07 0 0.07 0 0 0.07 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0.05 0 0 0 0.06 0.1 0.1 0.09
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2 1 2
Bud 0 0 1 0 1 0 5 2 6 8 3 6 0 0 0 1 0 0 0 2 3 1 4 10
Bud Neck 0 0 2 1 2 3 2 5 1 3 3 4 0 0 0 0 0 0 0 0 1 1 2 4
Bud Site 0 0 1 5 2 16 18 6 18 22 25 14 1 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 3 9 12 7 19 20 26 23 11 10 7 9 31 25 37 8 1 3 0 2 2 1 2 2
Endoplasmic Reticulum 0 2 1 0 0 1 0 0 0 0 0 0 1 0 2 1 1 1 0 0 0 1 2 3
Endosome 0 1 1 0 1 0 1 0 1 0 0 0 0 4 3 0 1 2 2 3 2 3 11 13
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 2 3 0 0 1 2
Mitochondria 24 41 20 11 16 101 142 72 200 230 133 73 17 1 7 3 3 5 3 2 3 4 5 4
Nucleus 205 221 372 204 308 348 403 352 308 300 190 201 273 210 237 55 49 18 187 222 334 120 159 145
Nuclear Periphery 1 0 3 0 2 8 8 2 6 6 1 2 8 1 0 1 0 0 0 0 1 1 1 2
Nucleolus 18 5 3 4 6 23 21 22 48 49 50 30 29 12 23 1 7 2 18 16 25 44 55 51
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 3 0 0 0 0 0 0 0 0 0 1
SpindlePole 2 3 28 8 23 8 22 29 10 10 5 6 2 4 2 1 0 0 0 2 7 4 6 14
Vac/Vac Membrane 0 5 2 0 5 2 3 3 1 2 0 1 2 4 6 4 3 1 7 7 24 20 30 27
Unique Cell Count 219 252 392 217 348 413 492 422 397 407 237 255 318 247 297 71 57 25 226 267 411 212 290 299
Labelled Cell Count 253 287 446 240 385 530 651 516 610 640 417 346 365 265 317 75 65 32 226 267 411 212 290 299


Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.3 5.4 5.5 5.2 5.7 4.6 4.8 5.1 4.1 3.9 3.8 4.5 5.9 6.8 5.9 7.7 8.2 7.2 6.2 5.3 5.6
Std Deviation (1e-4) 0.8 0.8 0.8 1.3 1.2 1.3 1.2 1.3 0.7 0.7 1.0 1.1 2.3 4.1 2.0 2.2 1.5 1.7 4.4 2.0 1.7
Intensity Change (Log2) – – – -0.09 0.05 -0.26 -0.2 -0.13 -0.44 -0.5 -0.55 -0.31 0.09 0.29 0.09 0.46 0.56 0.37 0.17 -0.05 0.01


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.1 1.6 1.3 1.6 1.7 -0.2 -0.5 -0.1 0.3 3.7 3.7 4.8 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 -0.3 0 0 5.4 0 0 0 0 0.1 -3.2 -1.8 0 0 0
Nucleus -0.5 -3.2 -4.9 -5.8 -5.2 -7.0 -8.2 -5.8 -6.3 -4.2 -4.3 -6.1 -5.0 -2.6 -4.5
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 3.7 0 0 0 0 5.3 3.3 4.8 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole -1.7 -0.3 0 0 -0.2 0 0 0 0 -4.3 -3.1 -4.1 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 3.7391 3.6222 3.4762 2.8641 3.2457 3.4165 2.2983 1.8755 1.8643 1.732 1.6846 1.7059 1.7602 1.0499 0.7854 1.3447 0.873 1.0108
Actin 0.0365 0.0006 0.0063 0 0.0105 0.002 0.0287 0.0002 0.0119 0.0391 0.0052 0.0005 0.0489 0 0.0121 0.001 0.0051 0.0007
Bud 0.0007 0.0001 0.0002 0 0.0002 0.0002 0.0005 0.0001 0.0013 0.0003 0.0003 0 0.0005 0 0.002 0.0017 0.0004 0.0001
Bud Neck 0.0028 0.0002 0.0009 0.0001 0.0008 0.0017 0.0026 0.0002 0.001 0.0008 0.0019 0.0006 0.0012 0.0001 0.0002 0.0012 0.0002 0.0007
Bud Periphery 0.0019 0.0001 0.0006 0 0.0003 0.0001 0.0009 0.0001 0.002 0.0006 0.0021 0.0002 0.0008 0 0.0036 0.0015 0.0006 0.0001
Bud Site 0.0128 0.0033 0.0009 0 0.0008 0.0008 0.0098 0.0025 0.0114 0.0017 0.0007 0.0001 0.0032 0.0001 0.0031 0.0011 0.0007 0.0001
Cell Periphery 0.0017 0 0.0003 0 0.0001 0.0001 0.0003 0 0.0003 0.0002 0.0008 0 0.0002 0 0.0001 0.0002 0.0001 0
Cytoplasm 0.0027 0.0002 0.0058 0.0001 0.0005 0.0068 0.0024 0.0001 0.0028 0.0003 0.0003 0.0001 0.0148 0.0001 0.0002 0.0793 0.0005 0.0003
Cytoplasmic Foci 0.0164 0.0019 0.0036 0 0.0102 0.0018 0.0122 0.0002 0.0069 0.0032 0.0019 0.0008 0.0183 0 0.0035 0.0019 0.008 0.0041
Eisosomes 0.001 0 0.0002 0 0.0001 0 0.0003 0 0.0001 0.0002 0.0002 0 0.0005 0 0.0001 0 0.0001 0
Endoplasmic Reticulum 0.0077 0 0.0052 0 0.0004 0.0004 0.0034 0 0.001 0.0004 0.0026 0.0004 0.0032 0 0.0001 0.0005 0.0015 0.0001
Endosome 0.0188 0.0015 0.0082 0 0.014 0.0062 0.0204 0.0003 0.0102 0.0135 0.0173 0.0035 0.0178 0 0.0015 0.0016 0.0292 0.0043
Golgi 0.0084 0.0018 0.006 0 0.0065 0.0025 0.0124 0.0004 0.0049 0.0047 0.0159 0.0027 0.0125 0 0.0016 0.0012 0.01 0.001
Lipid Particles 0.0273 0.0008 0.0036 0 0.0273 0.005 0.0173 0 0.0126 0.0049 0.0091 0.0013 0.0191 0 0.0018 0 0.0235 0.0031
Mitochondria 0.0161 0.0009 0.0138 0.0001 0.0058 0.0007 0.0081 0.0075 0.0133 0.0124 0.0288 0.0037 0.0183 0.0001 0.0017 0.0016 0.011 0.0005
None 0.0022 0 0.01 0 0.0003 0.0017 0.0023 0 0.0005 0.0002 0.0005 0.0001 0.0071 0 0.0002 0.0003 0.0007 0.0003
Nuclear Periphery 0.0077 0.0008 0.0044 0.0016 0.0019 0.001 0.0187 0.0008 0.0052 0.0011 0.0314 0.0116 0.007 0.0008 0.0095 0.0005 0.0085 0.0009
Nucleolus 0.0321 0.0236 0.0201 0.0173 0.1135 0.0899 0.0167 0.0192 0.019 0.0084 0.133 0.064 0.0238 0.0255 0.0219 0.0658 0.0653 0.0577
Nucleus 0.7697 0.9623 0.896 0.9779 0.7896 0.8724 0.8271 0.9674 0.8856 0.9015 0.7183 0.9043 0.7796 0.9717 0.9244 0.8387 0.7663 0.9203
Peroxisomes 0.0213 0.0004 0.006 0 0.0029 0.0011 0.0074 0.0001 0.002 0.0014 0.0008 0.0003 0.0101 0 0.0107 0.0001 0.0631 0.0028
Punctate Nuclear 0.0078 0.0011 0.0054 0.0028 0.0137 0.0053 0.0041 0.0006 0.0046 0.0003 0.0168 0.0041 0.0108 0.0016 0.0006 0.0009 0.0024 0.0025
Vacuole 0.0033 0.0002 0.0016 0 0.0004 0.0003 0.0026 0.0002 0.0022 0.0034 0.0049 0.0005 0.0011 0 0.0007 0.0006 0.0012 0.0002
Vacuole Periphery 0.0012 0.0001 0.0009 0 0.0003 0.0001 0.0019 0.0001 0.0013 0.0014 0.007 0.0013 0.0009 0 0.0002 0.0002 0.0018 0.0002

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 13.6713 12.6155 8.7762 14.5606 16.2959 12.7822 17.0406 18.1599 13.1306 17.022
Translational Efficiency 1.0072 1.2706 1.192 0.7497 0.7357 1.0597 0.9383 0.7876 0.8334 0.7027

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✔

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1911 1743 106 1249 1571 1556 72 1692 3482 3299 178 2941

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 657.46 829.14 1030.84 947.11 684.41 791.79 1007.99 987.55 669.62 811.52 1021.60 970.38
Standard Deviation 79.11 120.95 126.64 150.23 89.94 109.51 127.86 141.07 85.23 117.19 127.63 146.40
Intensity Change Log 2 — 0.334713 0.648845 0.526629 — 0.210257 0.558549 0.528993 — 0.272577 0.603497 0.527835

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000151 0.000539 0.002055 0.002161 0.000294 0.000468 0.001835 0.001270 0.000215 0.000505 0.001966 0.001648
Bud Neck 0.002981 0.011222 0.014101 0.035964 0.004088 0.009285 0.007083 0.042505 0.003480 0.010309 0.011262 0.039727
Bud Site 0.001000 0.001768 0.002029 0.015425 0.001550 0.003671 0.001397 0.005676 0.001248 0.002665 0.001773 0.009816
Cell Periphery 0.000132 0.000077 0.000069 0.000234 0.000108 0.000093 0.000079 0.000113 0.000122 0.000085 0.000073 0.000165
Cytoplasm 0.017853 0.005294 0.000481 0.015553 0.021933 0.004438 0.000676 0.009089 0.019694 0.004890 0.000560 0.011834
Cytoplasmic Foci 0.003515 0.000546 0.000060 0.005421 0.003351 0.001880 0.000048 0.004231 0.003441 0.001175 0.000056 0.004736
Eisosomes 0.000027 0.000025 0.000072 0.000040 0.000023 0.000031 0.000057 0.000034 0.000025 0.000028 0.000066 0.000037
Endoplasmic Reticulum 0.001000 0.003046 0.006305 0.006471 0.001953 0.003013 0.007010 0.005788 0.001430 0.003030 0.006590 0.006078
Endosome 0.000859 0.000512 0.000324 0.010811 0.000502 0.002599 0.000276 0.006117 0.000698 0.001496 0.000305 0.008110
Golgi 0.000584 0.000380 0.000241 0.008328 0.000287 0.001119 0.000194 0.005161 0.000450 0.000728 0.000222 0.006506
Lipid Particles 0.000966 0.000255 0.000255 0.000627 0.000585 0.000568 0.000217 0.000354 0.000794 0.000403 0.000240 0.000470
Mitochondria 0.003293 0.002676 0.001730 0.006507 0.002671 0.006373 0.001649 0.008324 0.003012 0.004420 0.001697 0.007552
Mitotic Spindle 0.004169 0.007201 0.001492 0.075261 0.001877 0.006210 0.012550 0.059120 0.003135 0.006733 0.005965 0.065975
None 0.026068 0.006578 0.003446 0.008026 0.019276 0.005240 0.001826 0.004373 0.023004 0.005947 0.002791 0.005924
Nuclear Periphery 0.000626 0.000701 0.000372 0.002542 0.000503 0.000972 0.000485 0.004519 0.000570 0.000829 0.000418 0.003680
Nuclear Periphery Foci 0.001013 0.000811 0.000323 0.003222 0.001073 0.000921 0.000330 0.002910 0.001040 0.000863 0.000326 0.003043
Nucleolus 0.096679 0.069390 0.063912 0.022666 0.057770 0.071666 0.053212 0.024878 0.079124 0.070464 0.059584 0.023939
Nucleus 0.836971* 0.887060* 0.898843* 0.757012* 0.880091* 0.875117* 0.907953* 0.803739* 0.856426* 0.881427* 0.902528* 0.783895*
Peroxisomes 0.001360 0.000440 0.001552 0.001323 0.000626 0.000976 0.001077 0.000463 0.001029 0.000693 0.001360 0.000828
Vacuole 0.000525 0.001020 0.002067 0.017064 0.001140 0.003826 0.001862 0.007919 0.000802 0.002343 0.001984 0.011803
Vacuole Periphery 0.000229 0.000461 0.000269 0.005341 0.000299 0.001533 0.000184 0.003417 0.000260 0.000966 0.000235 0.004234

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -9.01 -11.98 -11.33 -9.13 -0.09 -3.71 -6.33 -18.67 -13.12 2.95 -9.02 -12.32 -17.96 -14.00 2.83
Bud Neck -9.90 -7.05 -21.68 -16.23 -8.15 -6.02 -4.28 -24.97 -20.21 -14.20 -11.41 -8.08 -33.16 -25.82 -14.32
Bud Site -1.84 -4.07 -7.86 -7.28 -5.93 -2.01 -1.35 -6.71 -2.44 -5.99 -2.61 -3.87 -10.64 -7.53 -7.88
Cell Periphery 2.57 2.80 -2.21 -6.07 -7.04 0.86 0.51 0.59 -0.56 -0.15 2.51 2.57 -1.04 -4.32 -4.73
Cytoplasm 6.35 10.61 2.70 -3.79 -8.41 7.21 9.22 4.93 -3.70 -7.88 9.62 13.94 5.66 -5.09 -11.60
Cytoplasmic Foci 5.47 6.58 3.53 -2.36 -4.05 2.22 4.94 2.58 0.48 -3.30 5.48 8.22 4.44 -1.15 -5.09
Eisosomes 2.37 -7.66 -9.13 -12.03 4.26 -2.20 -5.99 -8.94 -3.12 3.83 -0.41 -9.79 -12.23 -9.46 6.10
Endoplasmic Reticulum -9.90 -7.22 -19.74 -11.04 -1.13 -3.67 -5.61 -11.17 -6.16 2.41 -9.06 -8.87 -21.11 -11.46 1.34
Endosome 1.41 2.14 -3.65 -4.41 -4.69 -3.51 0.71 -4.49 -0.07 -4.67 -2.20 2.28 -5.34 -3.44 -6.62
Golgi 1.12 1.65 -2.89 -3.31 -3.46 -2.24 0.49 -3.16 -1.47 -3.22 -0.96 1.71 -4.11 -3.54 -4.71
Lipid Particles 5.25 5.28 4.18 -2.35 -2.41 0.31 1.85 3.28 2.75 0.76 4.49 5.51 5.46 0.85 -0.89
Mitochondria 0.73 1.68 -2.74 -4.45 -7.79 -2.70 0.81 -3.03 0.46 -5.17 -1.75 1.80 -3.93 -1.70 -8.01
Mitotic Spindle -1.56 -0.07 -10.57 -9.77 -10.26 -2.50 -1.25 -11.34 -9.95 -4.10 -2.70 -1.22 -15.37 -13.88 -9.88
None 9.73 10.74 9.56 -0.33 -3.18 8.10 10.73 8.65 0.45 -6.92 12.61 14.49 13.02 0.27 -4.68
Nuclear Periphery -0.57 1.24 -12.37 -10.31 -12.42 -3.11 -1.65 -6.49 -5.33 -5.48 -2.41 -0.38 -9.90 -8.59 -9.37
Nuclear Periphery Foci 1.62 4.34 -1.14 -2.60 -4.79 0.60 1.69 -0.92 -1.40 -2.30 1.54 4.28 -1.33 -2.63 -4.83
Nucleolus 7.05 2.62 17.19 11.21 4.39 -3.33 1.00 8.76 12.28 1.85 3.76 2.46 19.20 16.65 4.67
Nucleus -8.66 -3.78 4.45 11.42 6.36 0.36 -1.38 7.94 7.63 5.51 -6.27 -3.62 7.83 13.52 8.05
Peroxisomes 3.47 -0.50 2.04 -2.46 3.25 -1.42 -2.05 1.98 4.05 4.32 2.41 -1.38 2.92 1.07 5.35
Vacuole -1.79 -2.35 -8.94 -8.35 -6.35 -2.73 -1.46 -7.52 -3.88 -0.47 -3.23 -2.09 -11.86 -8.89 -3.35
Vacuole Periphery -2.05 -1.88 -3.94 -3.36 -3.70 -3.81 0.70 -4.17 0.06 -4.37 -4.30 -1.01 -5.74 -2.57 -5.58
Standard name
Human Ortholog
Description Transcription factor; involved in regulation of cell cycle progression from G1 to S phase, forms a complex with Swi6p that binds to MluI cell cycle box regulatory element in promoters of DNA synthesis genes
Localization
Cell Percentages nucleus (96%)
Cell Cycle Regulation No
Subcompartmental Group nucleus-3

Mbp1

Mbp1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Mbp1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available